1. Lack of association of C-C chemokine receptor 5 Delta32 deletion status with rheumatoid arthritis, systemic lupus erythematosus, lupus nephritis, and disease severity
- Author
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Stefan P Berger, Gerrit van der Steege, Alexandre E. Voskuyl, Elisabeth Brouwer, Marc Bijl, Cees G. M. Kallenberg, Ruud G. L. de Sévaux, Sacha Gross, Gerjan Navis, Henk A. Martens, Ronald H. W. M. Derksen, Jo H. M. Berden, Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Translational Immunology Groningen (TRIGR), Lifestyle Medicine (LM), Groningen Kidney Center (GKC), Vascular Ageing Programme (VAP), Rheumatology, and CCA - Innovative therapy
- Subjects
Male ,Systemic disease ,Lupus nephritis ,Polymerase Chain Reaction ,Severity of Illness Index ,Arthritis, Rheumatoid ,Gene Frequency ,Lupus Erythematosus, Systemic ,Immunology and Allergy ,Renal disorder [IGMD 9] ,Sequence Deletion ,RISK ,education.field_of_study ,REVISED CRITERIA ,Middle Aged ,Connective tissue disease ,Rheumatoid arthritis ,Female ,Adult ,EXPRESSION ,medicine.medical_specialty ,Genotype ,Receptors, CCR5 ,Immunology ,Population ,Auto-immunity, transplantation and immunotherapy [N4i 4] ,Statistics, Nonparametric ,CLASSIFICATION ,Rheumatology ,Internal medicine ,medicine ,Humans ,education ,RHEUMATOID ARTHRITIS ,Alleles ,Genetic Association Studies ,Aged ,Autoimmune disease ,Chi-Square Distribution ,Base Sequence ,business.industry ,Delta 32 BASE-PAIR DELETION ,medicine.disease ,GENE ,LUPUS NEPHRITIS ,POLYMORPHISM ,Genotype frequency ,SYSTEMIC LUPUS ERYTHEMATOSUS ,C-C CHEMOKINE RECEPTOR 5 ,T-CELLS ,business ,CCR5 - Abstract
Objective.C-C chemokine receptor 5 (CCR5) plays an important role in inflammation. A 32 base-pair (Δ32) deletion in the CCR5 gene leads to a nonfunctional receptor. This deletion has been reported to have a protective effect on the development and progression of several autoimmune diseases. We investigated whether the Δ32 deletion is associated with disease susceptibility in a population of patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and lupus nephritis (LN); and whether it is associated with disease severity.MethodsDNA samples from 405 RA patients, 97 SLE patients, 113 LN patients, and 431 healthy controls were genotyped for the CCR5 Δ32 deletion. Differences in genotype frequencies were tested between patients and controls. Association of genotypes with disease severity was analyzed.ResultsGenotype frequencies of each group were in Hardy-Weinberg equilibrium. The genotype frequencies of patients did not differ significantly from controls (CCR5/Δ32, Δ32/Δ32: RA 18.3% and 1.2%, respectively; SLE 17.5% and 2.1%; LN 13.3% and 1.8%; controls 20.0% and 2.8%). However, there was a trend for lower Δ32 deletion allele frequency in LN patients compared to controls (p = 0.08). There was no significant association between the CCR5 status and disease severity in RA, SLE, or LN.Conclusion.Although an association with LN cannot be excluded, the CCR5 Δ32 deletion does not seem to be a disease susceptibility genotype for RA, SLE, or LN. No significant effect of the Δ32 deletion on disease severity was demonstrated.
- Published
- 2010