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1. Genetic dynamics in untreated CLL patients with either stable or progressive disease: a longitudinal study

Author

Ramassone, Alice, D’Argenio, Andrea, Veronese, Angelo, Basti, Alessio, Soliman, Shimaa Hassan AbdelAziz, Volinia, Stefano, Bassi, Cristian, Pagotto, Sara, Ferracin, Manuela, Lupini, Laura, Saccenti, Elena, Balatti, Veronica, Pepe, Felice, Rassenti, Laura Z, Innocenti, Idanna, Autore, Francesco, Marzetti, Laura, Mariani-Costantini, Renato, Kipps, Thomas J, Negrini, Massimo, Laurenti, Luca, and Visone, Rosa

Subjects
Biomedical and Clinical Sciences, Cardiovascular Medicine and Haematology, Rare Diseases, Cancer, Hematology, Clinical Research, Lymphoma, Aetiology, 2.1 Biological and endogenous factors, Biomarkers, Tumor, Chromosome Aberrations, Clonal Evolution, Disease Progression, Humans, Leukemia, Lymphocytic, Chronic, B-Cell, Longitudinal Studies, Mutation, Prognosis, Survival Rate, Chronic lymphocytic leukemia, Copy number variation, Nucleotide variation, Clonal evolution, Cardiorespiratory Medicine and Haematology, Oncology and Carcinogenesis, Cardiovascular medicine and haematology, Oncology and carcinogenesis
Abstract

Clonal evolution of chronic lymphocytic leukemia (CLL) often follows chemotherapy and is associated with adverse outcome, but also occurs in untreated patients, in which case its predictive role is debated. We investigated whether the selection and expansion of CLL clone(s) precede an aggressive disease shift. We found that clonal evolution occurs in all CLL patients, irrespective of the clinical outcome, but is faster during disease progression. In particular, changes in the frequency of nucleotide variants (NVs) in specific CLL-related genes may represent an indicator of poor clinical outcome.

Published
2019

2. Supplementary Table 1 from microRNAome Expression in Chronic Lymphocytic Leukemia: Comparison with Normal B-cell Subsets and Correlations with Prognostic and Clinical Parameters

Author

Negrini, Massimo, primary, Cutrona, Giovanna, primary, Bassi, Cristian, primary, Fabris, Sonia, primary, Zagatti, Barbara, primary, Colombo, Monica, primary, Ferracin, Manuela, primary, D'Abundo, Lucilla, primary, Saccenti, Elena, primary, Matis, Serena, primary, Lionetti, Marta, primary, Agnelli, Luca, primary, Gentile, Massimo, primary, Recchia, Anna Grazia, primary, Bossio, Sabrina, primary, Reverberi, Daniele, primary, Rigolin, Gianmatteo, primary, Calin, George A., primary, Sabbioni, Silvia, primary, Russo, Giandomenico, primary, Tassone, Pierfrancesco, primary, Morabito, Fortunato, primary, Ferrarini, Manlio, primary, and Neri, Antonino, primary

Published
2023
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4. Supplementary Table 2 from microRNAome Expression in Chronic Lymphocytic Leukemia: Comparison with Normal B-cell Subsets and Correlations with Prognostic and Clinical Parameters

Author

Negrini, Massimo, primary, Cutrona, Giovanna, primary, Bassi, Cristian, primary, Fabris, Sonia, primary, Zagatti, Barbara, primary, Colombo, Monica, primary, Ferracin, Manuela, primary, D'Abundo, Lucilla, primary, Saccenti, Elena, primary, Matis, Serena, primary, Lionetti, Marta, primary, Agnelli, Luca, primary, Gentile, Massimo, primary, Recchia, Anna Grazia, primary, Bossio, Sabrina, primary, Reverberi, Daniele, primary, Rigolin, Gianmatteo, primary, Calin, George A., primary, Sabbioni, Silvia, primary, Russo, Giandomenico, primary, Tassone, Pierfrancesco, primary, Morabito, Fortunato, primary, Ferrarini, Manlio, primary, and Neri, Antonino, primary

Published
2023
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5. Supplementary Figures 1 - 6 from microRNAome Expression in Chronic Lymphocytic Leukemia: Comparison with Normal B-cell Subsets and Correlations with Prognostic and Clinical Parameters

Author

Negrini, Massimo, primary, Cutrona, Giovanna, primary, Bassi, Cristian, primary, Fabris, Sonia, primary, Zagatti, Barbara, primary, Colombo, Monica, primary, Ferracin, Manuela, primary, D'Abundo, Lucilla, primary, Saccenti, Elena, primary, Matis, Serena, primary, Lionetti, Marta, primary, Agnelli, Luca, primary, Gentile, Massimo, primary, Recchia, Anna Grazia, primary, Bossio, Sabrina, primary, Reverberi, Daniele, primary, Rigolin, Gianmatteo, primary, Calin, George A., primary, Sabbioni, Silvia, primary, Russo, Giandomenico, primary, Tassone, Pierfrancesco, primary, Morabito, Fortunato, primary, Ferrarini, Manlio, primary, and Neri, Antonino, primary

Published
2023
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6. Additional lesions identified by genomic microarrays are associated with an inferior outcome in low‐risk chronic lymphocytic leukaemia patients.

Author

Rigolin, Gian Matteo, Traversa, Alice, Caputo, Viviana, Del Giudice, Ilaria, Bardi, Antonella, Saccenti, Elena, Raponi, Sara, Ilari, Caterina, Cafforio, Luciana, Giovannetti, Agnese, Pizzuti, Antonio, Guarini, Anna, Foà, Robin, and Cuneo, Antonio

Subjects
LYMPHOCYTIC leukemia, FLUORESCENCE in situ hybridization, CHRONIC leukemia, CHRONIC lymphocytic leukemia
Abstract

Summary: We explored the relevance of genomic microarrays (GM) in the refinement of prognosis in newly diagnosed low‐risk chronic lymphocytic leukaemia (CLL) patients as defined by isolated del(13q) or no lesions by a standard 4 probe fluorescence in situ hybridization (FISH) analysis. Compared to FISH, additional lesions were detected by GM in 27 of the 119 patients (22.7%). The concordance rate between FISH and GM was 87.4%. Discordant results between cytogenetic banding analysis (CBA) and GM were observed in 45/119 cases (37.8%) and were mainly due to the intrinsic characteristics of each technique. The presence of additional lesions by GM was associated with age > 65 years (p = 0.047), advanced Binet stage (p = 0.001), CLL‐IPI score (p < 0.001), a complex karyotype (p = 0.004) and a worse time‐to‐first treatment in multivariate analysis (p = 0.009). Additional lesions by GM were also significantly associated with a worse time‐to‐first treatment in the subset of patients with wild‐type TP53 and mutated IGHV (p = 0.025). In CLL patients with low‐risk features, the presence of additional lesions identified by GM helps to identify a subset of patients with a worse outcome that could be proposed for a risk‐adapted follow‐up and for early treatment including targeted agents within clinical trials. [ABSTRACT FROM AUTHOR]

Published
2023
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7. Monitoring Somatic Genetic Alterations in Circulating Cell-Free DNA/RNA of Patients with “Oncogene-Addicted” Advanced Lung Adenocarcinoma: A Real-World Clinical Study

Author

Lupini, Laura, primary, Roncarati, Roberta, additional, Belluomini, Lorenzo, additional, Lancia, Federica, additional, Bassi, Cristian, additional, D’Abundo, Lucilla, additional, Michilli, Angelo, additional, Guerriero, Paola, additional, Fasano, Alessandra, additional, Tiberi, Elisa, additional, Salamone, Andrea, additional, Cosi, Donato Michele, additional, Saccenti, Elena, additional, Tagliatti, Valentina, additional, Maestri, Iva, additional, Sabbioni, Silvia, additional, Volinia, Stefano, additional, Gafà, Roberta, additional, Lanza, Giovanni, additional, Frassoldati, Antonio, additional, and Negrini, Massimo, additional

Published
2022
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8. Diagnostic work-up for clinical and prognostic assessment of acute leukaemia

Author

Martinelli, Sara, Cibien, Francesca, Scarfò, Lydia, Ambrosio, Cristina, Formigaro, Luca, Daghia, Giulia, Sofritti, Olga, Rizzotto, Lara, Saccenti, Elena, Bardi, Antonella, Tammiso, Elisa, Volta, Eleonora, Ferrari, Luisa, Campioni, Diana, Dabusti, Melissa, Ciccone, Maria, Moretti, Sabrina, Tomasi, Paolo, Cavazzini, Francesco, Negrini, Massimo, Rigolin, Gian Matteo, and Cuneo, Antonio

Published
2012
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9. Molecular testing on bronchial washings for the diagnosis and predictive assessment of lung cancer

Author

Roncarati, Roberta, primary, Lupini, Laura, additional, Miotto, Elena, additional, Saccenti, Elena, additional, Mascetti, Susanna, additional, Morandi, Luca, additional, Bassi, Cristian, additional, Rasio, Debora, additional, Callegari, Elisa, additional, Conti, Valentina, additional, Rinaldi, Rosa, additional, Lanza, Giovanni, additional, Gafà, Roberta, additional, Papi, Alberto, additional, Frassoldati, Antonio, additional, Sabbioni, Silvia, additional, Ravenna, Franco, additional, Casoni, Gian L., additional, and Negrini, Massimo, additional

Published
2020
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10. In chronic lymphocytic leukaemia, SLAMF1 deregulation is associated with genomic complexity and independently predicts a worse outcome

Author

Rigolin, Gian Matteo, primary, Saccenti, Elena, additional, Melandri, Aurora, additional, Cavallari, Maurizio, additional, Urso, Antonio, additional, Rotondo, Francesco, additional, Betulla, Anita, additional, Tognolo, Lucia, additional, Bardi, Maria Antonella, additional, Rossini, Marika, additional, Tammiso, Elisa, additional, Bassi, Christian, additional, Cavazzini, Francesco, additional, Negrini, Massimo, additional, and Cuneo, Antonio, additional

Published
2020
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11. MOESM4 of Genetic dynamics in untreated CLL patients with either stable or progressive disease: a longitudinal study

Author

Ramassone, Alice, D’Argenio, Andrea, Veronese, Angelo, Basti, Alessio, Shimaa Soliman, Volinia, Stefano, Bassi, Cristian, Pagotto, Sara, Ferracin, Manuela, Lupini, Laura, Saccenti, Elena, Balatti, Veronica, Pepe, Felice, Rassenti, Laura, Idanna Innocenti, Autore, Francesco, Marzetti, Laura, Mariani-Costantini, Renato, Kipps, Thomas, Negrini, Massimo, Laurenti, Luca, and Visone, Rosa

Abstract

Additional file 4: Table S3 Genetic variants represented in Fig. 2.

Published
2019
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12. AJMQ860590_Supplemental_Material_CLN – Supplemental material for Evaluation of Care Processes and Health Care Utilization in Newly Implemented Medical Homes in Italy: A Population-Based Cross-sectional Study

Author

Alcusky, Matthew, Singer, David, Keith, Scott W., Hegarty, Sarah E., Lombardi, Marco, Saccenti, Elena, and Maio, Vittorio

Subjects
111799 Public Health and Health Services not elsewhere classified, 160807 Sociological Methodology and Research Methods, FOS: Health sciences, FOS: Sociology
Abstract

Supplemental material, AJMQ860590_Supplemental_Material_CLN for Evaluation of Care Processes and Health Care Utilization in Newly Implemented Medical Homes in Italy: A Population-Based Cross-sectional Study by Matthew Alcusky, David Singer, Scott W. Keith, Sarah E. Hegarty, Marco Lombardi, Elena Saccenti and Vittorio Maio in American Journal of Medical Quality

Published
2019
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15. DNA methylation of shelf, shore and open sea CpG positions distinguish high microsatellite instability from low or stable microsatellite status colon cancer stem cells

Author

Visone, Rosa, primary, Bacalini, Maria Giulia, additional, Franco, Simone Di, additional, Ferracin, Manuela, additional, Colorito, Maria Luisa, additional, Pagotto, Sara, additional, Laprovitera, Noemi, additional, Licastro, Danilo, additional, Marco, Mirco Di, additional, Scavo, Emanuela, additional, Bassi, Cristian, additional, Saccenti, Elena, additional, Nicotra, Annalisa, additional, Grzes, Maria, additional, Garagnani, Paolo, additional, Laurenzi, Vincenzo De, additional, Valeri, Nicola, additional, Mariani-Costantini, Renato, additional, Negrini, Massimo, additional, Stassi, Giorgio, additional, and Veronese, Angelo, additional

Published
2019
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16. MicroRNAs involvement in fludarabine refractory chronic lymphocytic leukemia

Author

Lupini Laura, Saccenti Elena, Ciccone Maria, Veronese Angelo, Cavazzini Francesco, Rizzotto Lara, Zagatti Barbara, Ferracin Manuela, Grilli Andrea, De Angeli Cristiano, Negrini Massimo, and Cuneo Antonio

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Fludarabine, is one of the most active single agents in the treatment of chronic lymphocytic leukemia (CLL). Over time, however, virtually all CLL patients become fludarabine-refractory. To elucidate whether microRNAs are involved in the development of fludarabine resistance, we analyzed the expression of 723 human miRNAs before and 5-days after fludarabine mono-therapy in 17 CLL patients which were classified as responder or refractory to fludarabine treatment based on NCI criteria. Results By comparing the expression profiles of these two groups of patients, we identified a microRNA signature able to distinguish refractory from sensitive CLLs. The expression of some microRNAs was also able to predict fludarabine resistance of 12 independent CLL patients. Among the identified microRNAs, miR-148a, miR-222 and miR-21 exhibited a significantly higher expression in non-responder patients either before and after fludarabine treatment. After performing messenger RNA expression profile of the same patients, the activation of p53-responsive genes was detected in fludarabine responsive cases only, therefore suggesting a possible mechanism linked to microRNA deregulation in non-responder patients. Importantly, inhibition of miR-21 and miR-222 by anti-miRNA oligonucleotides induced a significant increase in caspase activity in fludarabine-treated p53-mutant MEG-01 cells, suggesting that miR-21 and miR-222 up-regulation may be involved in the establishment of fludarabine resistance. Conclusions This is the first report that reveals the existence of a microRNA profile that differentiate refractory and sensitive CLLs, either before and after fludarabine mono-therapy. A p53 dysfunctional pathway emerged in refractory CLLs and could contribute in explaining the observed miRNA profile. Moreover, this work indicates that specific microRNAs can be used to predict fludarabine resistance and may potentially be used as therapeutic targets, therefore establishing an important starting point for future studies.

Published
2010
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17. In CLL, comorbidities and the complex karyotype are associated with an inferior outcome independently of CLL-IPI

Author

Rigolin, Gian Matteo, Cavallari, Maurizio, Quaglia, Francesca Maria, Formigaro, Luca, Lista, Enrico, Urso, Antonio, Guardalben, Emanuele, Liberatore, Carmine, Faraci, Danilo, Saccenti, Elena, Bassi, Cristian, Lupini, Laura, Bardi, Maria Antonella, Volta, Eleonora, Tammiso, Elisa, Melandri, Aurora, Negrini, Massimo, Cavazzini, Francesco, and Cuneo, Antonio

Published
2017
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18. The implementation of a Community Health Centre-based primary care model in Italy. The experience of the Case della Salute in the Emilia-Romagna Region

Author

ODONE, Anna, SACCANI, Elisa, CHIESA, VALENTINA, Brambilla, Antonio, Brianti, Ettore, Fabi, Massimo, Curcetti, Clara, Donatini, Andrea, Balestrino, Antonio, LOMBARDI, MARCO, ROSSI, Giuseppina, SACCENTI, Elena, SIGNORELLI, Carlo, Odone, Anna, Saccani, Elisa, Chiesa, Valentina, Brambilla, Antonio, Brianti, Ettore, Fabi, Massimo, Curcetti, Clara, Donatini, Andrea, Balestrino, Antonio, Lombardi, Marco, Rossi, Giuseppina, Saccenti, Elena, and Signorelli, Carlo

Subjects
Health Facility Size, Italy, Primary Health Care, General Practitioner, Environmental and Occupational Health, Public Health, Community Health Center, Family Practice, Delivery of Health Care, Regional Health Planning, Human
Abstract

BACKGROUND: The Comunity Health Centre (CHC) primary care model is a team-based health care delivery model intended to provide comprehensive and continuous medical care to patients within a defined community. The CHC, Case della Salute in Italian, model was introduced in the Emilia-Romagna Region in 2010.METHODS: We present updated data on the implementation on the CHC Case della Salute primary care model in the Emilia-Romagna Region.RESULTS: There are 67 operating CHCs in Emilia-Romagna (update March 2015); 26 small (39%), 24 medium (36%) and 17 large (25%). Since 2011 the number of operating CHCs has increased by 60%, reaching 55% of the target planned CHCs (n. = 122). There is, on average, one running CHC per 66.524 inhabitants. 16% of total general practitioners (GPs) and 8.4% of total family paediatricians working in Emilia-Romagna have their practice in CHCs. CHCs offer primary and specialist integrated care, prevention services, health education and social care.DISCUSSION: Although preliminary results suggest CHCs have fostered primary care's quality and efficiency, more research is needed to assess their impact on improving clinical, social and economic outcomes.

Published
2016

19. In chronic lymphocytic leukaemia, SLAMF1 deregulation is associated with genomic complexity and independently predicts a worse outcome.

Author

Rigolin, Gian Matteo, Saccenti, Elena, Melandri, Aurora, Cavallari, Maurizio, Urso, Antonio, Rotondo, Francesco, Betulla, Anita, Tognolo, Lucia, Bardi, Maria Antonella, Rossini, Marika, Tammiso, Elisa, Bassi, Christian, Cavazzini, Francesco, Negrini, Massimo, and Cuneo, Antonio

Subjects
*CHRONIC leukemia, *LYMPHOCYTIC leukemia, *IMMUNOGLOBULIN heavy chains, *P53 protein
Abstract

Summary: In a series of 349 patients with chronic lymphocytic leukaemia (CLL), we found lower levels of signalling lymphocytic activation molecule family member 1 (SLAMF1) expression in cases with highly complex karyotypes, as defined by the presence of five or more chromosomal abnormalities (CK5; P < 0·001) and with major chromosomal structural abnormalities (P < 0·001). SLAMF1 downregulation was significantly associated with advanced Binet Stage (P = 0·001), CD38 positivity (P < 0·001), high β2‐microglobulin levels (P < 0·001), immunoglobulin heavy chain variable region gene (IGHV) unmutated status (P < 0·001), 11q deletion (P < 0·001), tumour protein p53 (TP53) disruption (P = 0·011) and higher risk CLL International Prognostic Index categories (P < 0·001). Multivariate analysis showed that downregulated SLAMF1 levels had independent negative prognostic impact on time‐to‐first treatment (P < 0·001) and overall survival (P < 0·001). [ABSTRACT FROM AUTHOR]

Published
2021
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20. Evaluation of Care Processes and Health Care Utilization in Newly Implemented Medical Homes in Italy: A Population-Based Cross-sectional Study.

Author

Alcusky, Matthew, Singer, David, Keith, Scott W., Hegarty, Sarah E., Lombardi, Marco, Saccenti, Elena, and Maio, Vittorio

Abstract

In the Local Health Authority (LHA) of Parma, Emilia Romagna, Italy, 16 medical homes were established between 2011 and 2014. The authors implemented a 1-year (January 1, 2015, to December 31, 2015) cross-sectional population-based design to compare utilization and processes of care between medical homes and comparison practices using the Parma LHA administrative health care database. Residents (n = 372 396) attributed to a primary care physician practicing in a medical home as of January 1, 2015, were considered exposed to medical homes. Adjusted rates of emergency department (ED) use (incidence rate ratio [IRR] = 0.86; 95% CI = 0.82-0.90), potentially avoidable ED use (IRR = 0.78; 95% CI = 0.72-0.84), and hospitalization for chronic ambulatory care sensitive conditions (ACSCs; IRR = 0.87, 95% CI = 0.78-0.97) were lower among patients in medical homes. Performance on process of care measures favored the medical home group; however, associations were generally weak. Receipt of care in medical homes in Parma LHA was associated with lower rates of avoidable ED visits and hospitalizations for chronic ACSCs. [ABSTRACT FROM AUTHOR]

Published
2020
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21. Biological significance and prognostic/predictive impact of complex karyotype in chronic lymphocytic leukemia

Author

Cavallari, Maurizio, primary, Cavazzini, Francesco, additional, Bardi, Antonella, additional, Volta, Eleonora, additional, Melandri, Aurora, additional, Tammiso, Elisa, additional, Saccenti, Elena, additional, Lista, Enrico, additional, Quaglia, Francesca Maria, additional, Urso, Antonio, additional, Laudisi, Michele, additional, Menotti, Elisa, additional, Formigaro, Luca, additional, Dabusti, Melissa, additional, Ciccone, Maria, additional, Tomasi, Paolo, additional, Negrini, Massimo, additional, Cuneo, Antonio, additional, and Rigolin, Gian Matteo, additional

Published
2018
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22. In chronic lymphocytic leukaemia with complex karyotype, major structural abnormalities identify a subset of patients with inferior outcome and distinct biological characteristics

Author

Rigolin, Gian Matteo, primary, Saccenti, Elena, additional, Guardalben, Emanuele, additional, Cavallari, Maurizio, additional, Formigaro, Luca, additional, Zagatti, Barbara, additional, Visentin, Andrea, additional, Mauro, Francesca R., additional, Lista, Enrico, additional, Bassi, Cristian, additional, Lupini, Laura, additional, Quaglia, Francesca Maria, additional, Urso, Antonio, additional, Bardi, Maria Antonella, additional, Bonaldi, Laura, additional, Volta, Eleonora, additional, Tammiso, Elisa, additional, Ilari, Caterina, additional, Cafforio, Luciana, additional, Melandri, Aurora, additional, Cavazzini, Francesco, additional, Negrini, Massimo, additional, Semenzato, Gianpietro, additional, Trentin, Livio, additional, Foà, Robin, additional, and Cuneo, Antonio, additional

Published
2018
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23. Erratum to: Extensive next-generation sequencing analysis in chronic lymphocytic leukemia at diagnosis: clinical and biological correlations (J Hematol Oncol. (2016) 9 (88) DOI:10.1186/s13045-016-0320-z)

Author

Rigolin, Gian Matteo, Saccenti, Elena, Bassi, Cristian, Lupini, Laura, Quaglia, Francesca Maria, Cavallari, Maurizio, Martinelli, Sara, Formigaro, Luca, Lista, Enrico, Bardi, Maria Antonella, Volta, Eleonora, Tammiso, Elisa, Melandri, Aurora, Urso, Antonio, Cavazzini, Francesco, Negrini, Massimo, and Cuneo, Antonio

Subjects
Hematology, Molecular Biology, Oncology, Cancer Research, NO
Published
2016

24. Sensitive and specific detection of lung cancer DNA in bronchial washing by a novel methylation-specific droplet digital PCR

Author

Casoni, Gian Luca, primary, Miotto, Elena, additional, Saccenti, Elena, additional, Mascetti, Susanna, additional, Rasio, Debora, additional, Marchi, Irene, additional, Rinaldi, Rosa, additional, Lanza, Giovanni, additional, Papi, Alberto, additional, Sabbioni, Silvia, additional, Ravenna, Franco, additional, and Negrini, Massimo, additional

Published
2017
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25. An extensive molecular cytogenetic characterization in high-risk chronic lymphocytic leukemia identifies karyotype aberrations and TP53 disruption as predictors of outcome and chemorefractoriness

Author

Rigolin, Gian Matteo, primary, Formigaro, Luca, additional, Cavallari, Maurizio, additional, Quaglia, Francesca Maria, additional, Lista, Enrico, additional, Urso, Antonio, additional, Guardalben, Emanuele, additional, Martinelli, Sara, additional, Saccenti, Elena, additional, Bassi, Cristian, additional, Lupini, Laura, additional, Bardi, Maria Antonella, additional, Volta, Eleonora, additional, Tammiso, Elisa, additional, Melandri, Aurora, additional, Negrini, Massimo, additional, Cavazzini, Francesco, additional, and Cuneo, Antonio, additional

Published
2017
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27. Chronic lymphocytic leukemia: assessing pathogenesis and prognosis by modern molecular cytogenetic studies and microRNAs expression

Author

Saccenti, Elena

Subjects
chronic, lymphocytic, immune system diseases, hemic and lymphatic diseases, MED/15 Malattie del sangue, leukemia, Settore MED/15 - Malattie del Sangue
Abstract

Chronic lymphocytic leukemia (CLL) is a B-cell clonal lymphoprolipherative disorder characterized by the accumulation of small lymphocytes in the peripheral blood, bone marrow and lymph nodes deriving from the transformation of CD5+ B-cell. Despite a homogeneous immunophenotype consisting of CD19+, CD20+, CD5+ and CD23+, CLL is clinically heterogeneous. Several adverse prognostic features have been identified including stage, CD38 positivity, the unmutated configuration of the variable region of the immunoglobulin heavy chain gene (IGHV), ZAP70 positivity, chromosome aberrations and molecular abnormalities. Detailed immunophenotypic and genetic analysis allowed for the identification of a number of markers of activation and genetic instability, some of which are gaining relevance in clinical practice to predict outcome. Cell surface CD38 is one of these markers since it is an indicator of cell activation and proliferation that may prelude clonal evolution and worse clinical outcome. We therefore studied the biological and clinical significance of the presence of genetic heterogeneity in the minor CD38+ leukemic population, in a cohort of untreated low-risk CD38-negative CLL patients, defined by the presence of

Published
2014

28. Erratum to: Extensive next-generation sequencing analysis in chronic lymphocytic leukemia at diagnosis: clinical and biological correlations

Author

Rigolin, Gian Matteo, primary, Saccenti, Elena, additional, Bassi, Cristian, additional, Lupini, Laura, additional, Quaglia, Francesca Maria, additional, Cavallari, Maurizio, additional, Martinelli, Sara, additional, Formigaro, Luca, additional, Lista, Enrico, additional, Bardi, Maria Antonella, additional, Volta, Eleonora, additional, Tammiso, Elisa, additional, Melandri, Aurora, additional, Urso, Antonio, additional, Cavazzini, Francesco, additional, Negrini, Massimo, additional, and Cuneo, Antonio, additional

Published
2016
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29. Extensive next-generation sequencing analysis in chronic lymphocytic leukemia at diagnosis: clinical and biological correlations

Author

Rigolin, Gian Matteo, primary, Saccenti, Elena, additional, Bassi, Cristian, additional, Lupini, Laura, additional, Quaglia, Francesca Maria, additional, Cavallari, Maurizio, additional, Martinelli, Sara, additional, Formigaro, Luca, additional, Lista, Enrico, additional, Bardi, Maria Antonella, additional, Volta, Eleonora, additional, Tammiso, Elisa, additional, Melandri, Aurora, additional, Urso, Antonio, additional, Cavazzini, Francesco, additional, Negrini, Massimo, additional, and Cuneo, Antonio, additional

Published
2016
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30. Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters

Author

Rigolin, Gian Matteo, Cibien, Francesca, Martinelli, Sara, Formigaro, Luca, Rizzotto, Lara, Tammiso, Elisa, Saccenti, Elena, Bardi, Antonella, Cavazzini, Francesco, Ciccone, Maria, Nichele, Ilaria, Pizzolo, Giovanni, Zaja, Francesco, Fanin, Renato, Galieni, Piero, Dalsass, Alessia, Mestichelli, Francesca, Testa, Nicoletta, Negrini, Massimo, and Cuneo, Antonio

Published
2012
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31. Diagnostic workup for clinical and prognostic assessment of acute leukemia

Author

Martinelli, A, Cibien, Francesca, Scrfò, L, Ambrosio, Cristina, Formigaro, Luca, Daghia, Giulia, Sofritti, Olga, Rizzotto, Lara, Saccenti, Elena, Bardi, A, Tammiso, Elisa, Ferrari, L, Campioni, Diana, Dabusti, M, Ciccone, M, Moretti, S, Tomasi, P, Cavazzini, Francesco, Negrini, Massimo, Rigolin, Gian Matteo, and Cuneo, Antonio

Subjects
acute leukaemia, diagnosis, NO
Published
2011

33. Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia: Clinical and biologic correlations

Author

Rigolin, Gian Matteo, primary, del Giudice, Ilaria, additional, Formigaro, Luca, additional, Saccenti, Elena, additional, Martinelli, Sara, additional, Cavallari, Maurizio, additional, Lista, Enrico, additional, Tammiso, Elisa, additional, Volta, Eleonora, additional, Lupini, Laura, additional, Bassi, Cristian, additional, Bardi, Antonella, additional, Sofritti, Olga, additional, Daghia, Giulia, additional, Cavazzini, Francesco, additional, Marinelli, Marilisa, additional, Tavolaro, Simona, additional, Guarini, Anna, additional, Negrini, Massimo, additional, Foà, Robin, additional, and Cuneo, Antonio, additional

Published
2015
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34. Abstract 3964: How to fish a good micro-marker out from a worthless lake: The case of cell-free miR-181a-5p and breast cancer

Author

Ferracin, Manuela, primary, Lupini, Laura, additional, Salamon, Irene, additional, Saccenti, Elena, additional, Zagatti, Barabara, additional, Mangolini, Alessandra, additional, Zanzi, Maria Vittoria, additional, Carcoforo, Paolo, additional, Rocchi, Andrea, additional, Cavallesco, Giorgio, additional, Frassoldati, Antonio, additional, Hollingsworth, Alan B., additional, and Negrini, Massimo, additional

Published
2015
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35. Diagnostic and prognostic microRNAs in the serum of breast cancer patients measured by droplet digital PCR

Author

Mangolini, Alessandra, primary, Ferracin, Manuela, additional, Zanzi, Maria Vittoria, additional, Saccenti, Elena, additional, Ebnaof, Sayda Omer, additional, Poma, Valentina Vultaggio, additional, Sanz, Juana M., additional, Passaro, Angela, additional, Pedriali, Massimo, additional, Frassoldati, Antonio, additional, Querzoli, Patrizia, additional, Sabbioni, Silvia, additional, Carcoforo, Paolo, additional, Hollingsworth, Alan, additional, and Negrini, Massimo, additional

Published
2015
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36. Absolute quantification of cell-free microRNAs in cancer patients

Author

Ferracin, Manuela, primary, Lupini, Laura, additional, Salamon, Irene, additional, Saccenti, Elena, additional, Zanzi, Maria Vittoria, additional, Rocchi, Andrea, additional, Da Ros, Lucia, additional, Zagatti, Barbara, additional, Musa, Gentian, additional, Bassi, Cristian, additional, Mangolini, Alessandra, additional, Cavallesco, Giorgio, additional, Frassoldati, Antonio, additional, Volpato, Stefano, additional, Carcoforo, Paolo, additional, Hollingsworth, Alan B., additional, and Negrini, Massimo, additional

Published
2015
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38. LATE APPEARANCE of 14q32/IGH TRANSLOCATION In CHRONIC LYMPHOCYTIC LEUKEMIA

Author

Cavazzini, Francesco, Rigolin, Gian Matteo, Rizzotto, Lara, Antonella, Bardi, Tammiso, Elisa, Elisa, Pezzolo, Eleonora, Volta, Dalila De Pasquale, Dabusti, Melissa, Saccenti, Elena, Ciccone, Maria, Lydia, Scarfò, Francesca, Cibien, Sofritti, Olga, Daghia, Giulia, Martinelli, Sara, and Cuneo, Antonio

Published
2010

40. microRNAome Expression in Chronic Lymphocytic Leukemia: Comparison with Normal B-cell Subsets and Correlations with Prognostic and Clinical Parameters

Author

Negrini, Massimo, primary, Cutrona, Giovanna, additional, Bassi, Cristian, additional, Fabris, Sonia, additional, Zagatti, Barbara, additional, Colombo, Monica, additional, Ferracin, Manuela, additional, D'Abundo, Lucilla, additional, Saccenti, Elena, additional, Matis, Serena, additional, Lionetti, Marta, additional, Agnelli, Luca, additional, Gentile, Massimo, additional, Recchia, Anna Grazia, additional, Bossio, Sabrina, additional, Reverberi, Daniele, additional, Rigolin, Gianmatteo, additional, Calin, George A., additional, Sabbioni, Silvia, additional, Russo, Giandomenico, additional, Tassone, Pierfrancesco, additional, Morabito, Fortunato, additional, Ferrarini, Manlio, additional, and Neri, Antonino, additional

Published
2014
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42. Genetic subclonal complexity and miR125a-5p down-regulation identify a subset of patients with inferior outcome in low-risk CLL patients

Author

Rigolin, Gian Matteo, primary, Saccenti, Elena, additional, Rizzotto, Lara, additional, Ferracin, Manuela, additional, Martinelli, Sara, additional, Formigaro, Luca, additional, Cibien, Francesca, additional, Cavallari, Maurizio, additional, Lista, Enrico, additional, Daghia, Giulia, additional, Sofritti, Olga, additional, Ciccone, Maria, additional, Cavazzini, Francesco, additional, Lupini, Laura, additional, Bassi, Cristian, additional, Zagatti, Barbara, additional, Negrini, Massimo, additional, and Cuneo, Antonio, additional

Published
2013
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43. BCR/ABL1-positive acute lymphoblastic leukemia relapsing asBCR/ABL1-negative acute lymphoblastic leukemia

Author

Rizzotto, Lara, primary, Saccenti, Elena, additional, Sofritti, Olga, additional, Daghia, Giulia, additional, Volta, Eleonora, additional, Caprini, Elisabetta, additional, Lupini, Laura, additional, Tammiso, Elisa, additional, Bardi, Antonella, additional, Lista, Enrico, additional, Ciccone, Maria, additional, Russo, Giandomenico, additional, Negrini, Massimo, additional, Cuneo, Antonio, additional, and Rigolin, Gian Matteo, additional

Published
2013
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44. Chromosome Aberrations by Conventional Karyotyping in Chronic Lymphocytic Leukemia Carrying No Aberration by Fluorescence in Situ Hybridization: Correlation with Prognostic Parameters and Clinical Features

Author

Rigolin, Gian Matteo, primary, Cibien, Francesca, additional, Martinelli, Sara, additional, Luca, Formigaro, additional, Bardi, Antonella, additional, Rizzotto, Lara, additional, Tammiso, Elisa, additional, Saccenti, Elena, additional, Cavazzini, Francesco, additional, Ciccone, Maria, additional, Nichele, Ilaria, additional, Pizzolo, Giovanni, additional, Zaja, Francesco, additional, Fanin, Renato, additional, Galieni, Piero, additional, Dalsass, Alessia, additional, Mestichelli, Francesca, additional, Testa, Nicoletta, additional, Negrini, Massimo, additional, and Cuneo, Antonio, additional

Published
2011
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45. Clonal evolution including 14q32/IGHtranslocations in chronic lymphocytic leukemia: analysis of clinicobiologic correlations in 105 patients

Author

Cavazzini, Francesco, primary, Rizzotto, Lara, additional, Sofritti, Olga, additional, Daghia, Giulia, additional, Cibien, Francesca, additional, Martinelli, Sara, additional, Ciccone, Maria, additional, Saccenti, Elena, additional, Dabusti, Melissa, additional, Elkareem, Abbas Awad, additional, Bardi, Antonella, additional, Tammiso, Elisa, additional, Cuneo, Antonio, additional, and Rigolin, Gian Matteo, additional

Published
2011
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46. Late Appearance of 14q32/IGH Translocation in Chronic Lumphocytic Leukemia

Author

Cavazzini, Francesco, primary, Rigolin, Gian Matteo, additional, Rizzotto, Lara, additional, Bardi, Antonella, additional, Tammiso, Elisa, additional, Pezzolo, Elisa, additional, Volta, Eleonora, additional, De Pasquale, Dalila, additional, Dabusti, Melissa, additional, Saccenti, Elena, additional, Ciccone, Maria, additional, Scarfò, Lydia, additional, Cibien, Francesca, additional, Sofritti, Olga, additional, Daghia, Giulia, additional, Martinelli, Sara, additional, and Cuneo, Antonio, additional

Published
2010
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47. MicroRNAs involvement in fludarabine refractory chronic lymphocytic leukemia

Author

Ferracin, Manuela, primary, Zagatti, Barbara, additional, Rizzotto, Lara, additional, Cavazzini, Francesco, additional, Veronese, Angelo, additional, Ciccone, Maria, additional, Saccenti, Elena, additional, Lupini, Laura, additional, Grilli, Andrea, additional, De Angeli, Cristiano, additional, Negrini, Massimo, additional, and Cuneo, Antonio, additional

Published
2010
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48. Clonal evolution including 14q32/ IGH translocations in chronic lymphocytic leukemia: analysis of clinicobiologic correlations in 105 patients.

Author

Cavazzini, Francesco, Rizzotto, Lara, Sofritti, Olga, Daghia, Giulia, Cibien, Francesca, Martinelli, Sara, Ciccone, Maria, Saccenti, Elena, Dabusti, Melissa, Elkareem, Abbas Awad, Bardi, Antonella, Tammiso, Elisa, Cuneo, Antonio, and Rigolin, Gian Matteo

Subjects
CHROMOSOMAL translocation, LYMPHOCYTIC leukemia, IMMUNOGLOBULINS, FLUORESCENCE in situ hybridization, BONE marrow, LYMPH nodes, GENETICS
Abstract

To better define the significance of clonal evolution (CE) including 14q32 translocations involving the immunoglobulin heavy chain gene ( IGH) in chronic lymphocytic leukemia (CLL), 105 patients were analyzed sequentially by fluorescence in situ hybridization (FISH) with the following panel of probes: 13q14/D13S25, 11q22/ ATM, 17p13/ TP53, ##12-centromere and 14q32/ IGH break-apart probe. CE was observed in 15/105 patients after 24-170 months (median 64). Recurring aberrations at CE were 14q32/ IGH translocation in seven patients; other aberrations were 17p −, 11q −, biallelic 13q − and 14q32 deletion. CE was detected in 15/58 pre-treated patients; in contrast, none of 47 untreated patients developed CE ( p < 0.0001). In two cases the appearance of 14q32/ IGH translocation was first detected in the bone marrow (BM) or in the lymph node (LN) and 13-58 months later in the peripheral blood (PB). ZAP70 ++ and high-risk cytogenetics predicted for the occurrence of CE with borderline statistical significance ( p == 0.055 and 0.07, respectively). Shorter time to first treatment (TTT) and time to chemorefractoriness (TTCR) were noted in 15 patients with CE when compared to patients without CE (TTT: 35 vs. 71 months, p == 0.0033 and TTCR: 34 vs. 86 months, p == 0.0046, respectively). Survival after the development of CE was 32 months (standard error 8.5). We arrived at the following conclusions: (i) 14q32/ IGH translocation may represent one of the most frequent aberrations acquired during the natural history of CLL and (ii) it may be detected earlier in BM or LN samples; (iii) CE including 14q32/ IGH translocation occurs in pre-treated patients with short TTT and TTCR; (iii) survival after CE is relatively short. [ABSTRACT FROM AUTHOR]

Published
2012
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49. MicroRNAs involvement in fludarabine refractorychronic lymphocytic leukemia.

Author

Ferracin, Manuela, Zagatti, Barbara, Rizzotto, Lara, Cavazzini, Francesco, Veronese, Angelo, Ciccone, Maria, Saccenti, Elena, Lupini, Laura, Grilli, Andrea, De Angeli, Cristiano, Negrini, Massimo, and Cuneo, Antonio

Subjects
CHRONIC lymphocytic leukemia treatment, LEUKEMIA, FLUDARABINE, DRUG resistance in cancer cells, CANCER patients, GENE expression
Abstract

Background: Fludarabine, is one of the most active single agents in the treatment of chronic lymphocytic leukemia (CLL). Over time, however, virtually all CLL patients become fludarabine-refractory. To elucidate whether microRNAs are involved in the development of fludarabine resistance, we analyzed the expression of 723 human miRNAs before and 5-days after fludarabine mono-therapy in 17 CLL patients which were classified as responder or refractory to fludarabine treatment based on NCI criteria. Results: By comparing the expression profiles of these two groups of patients, we identified a microRNA signature able to distinguish refractory from sensitive CLLs. The expression of some microRNAs was also able to predict fludarabine resistance of 12 independent CLL patients. Among the identified microRNAs, miR-148a, miR-222 and miR-21 exhibited a significantly higher expression in non-responder patients either before and after fludarabine treatment. After performing messenger RNA expression profile of the same patients, the activation of p53- responsive genes was detected in fludarabine responsive cases only, therefore suggesting a possible mechanism linked to microRNA deregulation in non-responder patients. Importantly, inhibition of miR-21 and miR-222 by antimiRNA oligonucleotides induced a significant increase in caspase activity in fludarabine-treated p53-mutant MEG-01 cells, suggesting that miR-21 and miR-222 up-regulation may be involved in the establishment of fludarabine resistance. Conclusions: This is the first report that reveals the existence of a microRNA profile that differentiate refractory and sensitive CLLs, either before and after fludarabine mono-therapy. A p53 dysfunctional pathway emerged in refractory CLLs and could contribute in explaining the observed miRNA profile. Moreover, this work indicates that specific microRNAs can be used to predict fludarabine resistance and may potentially be used as therapeutic targets, therefore establishing an important starting point for future studies. [ABSTRACT FROM AUTHOR]

Published
2010
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50. BCR/ABL1-positive acute lymphoblastic leukemia relapsing as BCR/ABL1-negative acute lymphoblastic leukemia.

Author

Rizzotto, Lara, Saccenti, Elena, Sofritti, Olga, Daghia, Giulia, Volta, Eleonora, Caprini, Elisabetta, Lupini, Laura, Tammiso, Elisa, Bardi, Antonella, Lista, Enrico, Ciccone, Maria, Russo, Giandomenico, Negrini, Massimo, Cuneo, Antonio, and Rigolin, Gian Matteo

Subjects
*LYMPHOBLASTIC leukemia, *CHROMOSOME abnormalities, *PATIENTS
Abstract

A letter to the editor is presented which is concerned with the case of a patient with BCR/ABL1-positive acute leukemia who relapsed with BCR/ABL1-negative acute lymphoblastic leukemia.

Published
2013
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