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194 results on '"Sabatini, Stefania"'

21. The Impact of Spermidine on C2C12 Myoblasts Proliferation, Redox Status and Polyamines Metabolism under H 2 O 2 Exposure.

Author

Ceci, Roberta, Duranti, Guglielmo, Giuliani, Stefano, Rossi, Marianna Nicoletta, Dimauro, Ivan, Sabatini, Stefania, Mariottini, Paolo, and Cervelli, Manuela

Subjects
MYOBLASTS, SATELLITE cells, SPERMIDINE, AMINE oxidase, POLYAMINES, OXIDANT status, MUSCLE cells
Abstract

A central feature of the skeletal muscle is its ability to regenerate through the activation, by environmental signals, of satellite cells. Once activated, these cells proliferate as myoblasts, and defects in this process profoundly affect the subsequent process of regeneration. High levels of reactive oxygen species such as hydrogen peroxide (H2O2) with the consequent formation of oxidized macromolecules increase myoblasts' cell death and strongly contribute to the loss of myoblast function. Recently, particular interest has turned towards the beneficial effects on muscle of the naturally occurring polyamine spermidine (Spd). In this work, we tested the hypothesis that Spd, upon oxidative challenge, would restore the compromised myoblasts' viability and redox status. The effects of Spd in combination with aminoguanidine (Spd-AG), an inhibitor of bovine serum amine oxidase, on murine C2C12 myoblasts treated with a mild dose of H2O2 were evaluated by analyzing: (i) myoblast viability and recovery from wound scratch; (ii) redox status and (iii) polyamine (PAs) metabolism. The treatment of C2C12 myoblasts with Spd-AG increased cell number and accelerated scratch wound closure, while H2O2 exposure caused redox status imbalance and cell death. The combined treatment with Spd-AG showed an antioxidant effect on C2C12 myoblasts, partially restoring cellular total antioxidant capacity, reducing the oxidized glutathione (GSH/GSSG) ratio and increasing cell viability through a reduction in cell death. Moreover, Spd-AG administration counteracted the induction of polyamine catabolic genes and PA content decreased due to H2O2 challenges. In conclusion, our data suggest that Spd treatment has a protective role in skeletal muscle cells by restoring redox balance and promoting recovery from wound scratches, thus making myoblasts able to better cope with an oxidative insult. [ABSTRACT FROM AUTHOR]

Published
2022
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24. Can Physical Activity Influence Human Gut Microbiota Composition Independently of Diet? A Systematic Review

Author

Dorelli, Barbara, primary, Gallè, Francesca, additional, De Vito, Corrado, additional, Duranti, Guglielmo, additional, Iachini, Matteo, additional, Zaccarin, Matteo, additional, Preziosi Standoli, Jacopo, additional, Ceci, Roberta, additional, Romano, Ferdinando, additional, Liguori, Giorgio, additional, Romano Spica, Vincenzo, additional, Sabatini, Stefania, additional, Valeriani, Federica, additional, and Cattaruzza, Maria Sofia, additional

Published
2021
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25. Moringa oleifera Leaf Extract Protects C2C12 Myotubes against H2O2-Induced Oxidative Stress.

Author

Ceci, Roberta, Maldini, Mariateresa, Olson, Mark E., Crognale, Domenico, Horner, Katy, Dimauro, Ivan, Sabatini, Stefania, and Duranti, Guglielmo

Abstract

The imbalance between reactive oxygen species (ROS) production and antioxidant defense systems leads to macromolecule and tissue damage as a result of cellular oxidative stress. This phenomenon is considered a key factor in fatigue and muscle damage following chronic or highintensity physical exercise. In the present study, the antioxidant effect of Moringa oleifera leaf extract (MOLE) was evaluated in C2C12 myotubes exposed to an elevated hydrogen peroxide (H2O2 ) insult. The capacity of the extract to influence the myotube redox status was evaluated through an analysis of the total antioxidant capacity (TAC), glutathione homeostasis (GSH and GSSG), total free thiols (TFT), and thioredoxin (Trx) activity, as well as the enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and transferase (GST). Moreover, the ability of MOLE to mitigate the stress-induced peroxidation of lipids and oxidative damage (TBARS and protein carbonyls) was also evaluated. Our data demonstrate that MOLE pre-treatment mitigates the highly stressful effects of H2O2 in myotubes (1 mM) by restoring the redox status (TFT, Trx, and GSH/GSSG ratio) and increasing the antioxidant enzymatic system (CAT, SOD, GPx, GST), thereby significantly reducing the TBARs and PrCAR levels. Our study provides evidence that MOLE supplementation has antioxidant potential, allowing myotubes better able to cope with an oxidative insult and, therefore, could represent a useful nutritional strategy for the preservation of muscle well-being. [ABSTRACT FROM AUTHOR]

Published
2022
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27. Architectural Competition for Akama National Forest Park

Author

Departament Forestal del Ministeri d'Agricultura, Desenvolupament Rural i Medi Ambient, Universitat Politècnica de Catalunya. Departament de Representació Arquitectònica, Christodoulou, Marilena, Bilurrina Camps, Marcel, Sabatini, Stefania, Franceschini, Roberto, Departament Forestal del Ministeri d'Agricultura, Desenvolupament Rural i Medi Ambient, Universitat Politècnica de Catalunya. Departament de Representació Arquitectònica, Christodoulou, Marilena, Bilurrina Camps, Marcel, Sabatini, Stefania, and Franceschini, Roberto

Abstract

Menció, Postprint (published version)

Published
2020

29. Emerging Role for Linear and Circular Spermine Oxidase RNAs in Skeletal Muscle Physiopathology

Author

Reinoso-Sánchez, Jonathan Fernando, primary, Baroli, Giulia, additional, Duranti, Guglielmo, additional, Scaricamazza, Silvia, additional, Sabatini, Stefania, additional, Valle, Cristiana, additional, Morlando, Mariangela, additional, Casero, Robert Anthony Casero, additional, Bozzoni, Irene, additional, Mariottini, Paolo, additional, Ceci, Roberta, additional, and Cervelli, Manuela, additional

Published
2020
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31. The Phosphodiesterase Type 5 Inhibitor Sildenafil Improves DNA Stability and Redox Homeostasis in Systemic Sclerosis Fibroblasts Exposed to Reactive Oxygen Species

Author

Di Luigi, Luigi, primary, Duranti, Guglielmo, additional, Antonioni, Ambra, additional, Sgrò, Paolo, additional, Ceci, Roberta, additional, Crescioli, Clara, additional, Sabatini, Stefania, additional, Lenzi, Andrea, additional, Caporossi, Daniela, additional, Del Galdo, Francesco, additional, Dimauro, Ivan, additional, and Antinozzi, Cristina, additional

Published
2020
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33. Corrigendum to “Endurance training improves plasma superoxide dismutase activity in healthy elderly” [Mechanisms of Ageing and Development 185 (2020) 111190]

Author

Ceci, Roberta, primary, Duranti, Guglielmo, additional, Di Filippo, Ester Sara, additional, Bondi, Danilo, additional, Verratti, Vittore, additional, Doria, Christian, additional, Caporossi, Daniela, additional, Sabatini, Stefania, additional, Dimauro, Ivan, additional, and Pietrangelo, Tiziana, additional

Published
2020
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44. Resistance training and redox homeostasis: Correlation with age-associated genomic changes

Author

Dimauro, Ivan, Scalabrin, Mattia, Fantini, Cristina, Graziolo, Elisa, Beltran Valls, Maria Reyes, Mercatelli, Neri, Parisi, Attilio, Sabatini, Stefania, and Di Luigi, Luigi

Subjects
MnSOD, Telomeres, DNA methylation, Myeloperoxidase, TrxR1
Abstract

Regular physical activity is effective as prevention and treatment for different chronic conditions related to the ageing processes. In fact, a sedentary lifestyle has been linked to a worsening of cellular ageing biomarkers such as telomere length (TL) and/or specific epigenetic changes (e.g. DNA methylation), with increase of the propensity to aging-related diseases and premature death. Extending our previous findings, we aimed to test the hypothesis that 12 weeks of low frequency, moderate intensity, explosive-type resistance training (EMRT) may attenuate age-associated genomic changes. To this aim, TL, global DNA methylation, TRF2, Ku80, SIRT1, SIRT2 and global protein acetylation, as well as other proteins involved in apoptotic pathway (Bcl-2, Bax and Caspase-3), antioxidant response (TrxR1 and MnSOD) and oxidative damage (myeloperoxidase) were evaluated before and after EMRT in whole blood or peripheral mononuclear cells (PBMCs) of elderly subjects. Our findings confirm the potential of EMRT to induce an adaptive change in the antioxidant protein systems at systemic level and suggest a putative role of resistance training in the reduction of global DNA methylation. Moreover, we observed that EMRT counteracts the telomeres’ shortening in a manner that proved to be directly correlated with the amelioration of redox homeostasis and efficacy of training regime, evaluated as improvement of both muscle's power/strength and functional parameters. This work was supported by grants from MIUR (PRIN2012) to DC and from University of Rome “Foro Italico” (Research Grant 2013) to LDL. Further, we thank the “Veronesi Foundation” for the fellowships given to Dr. Ivan Dimauro

Published
2016

49. Physical exercise, nutrition and hormones: three pillars to fight sarcopenia.

Author

Sgrò, Paolo, Sansone, Massimiliano, Sansone, Andrea, Sabatini, Stefania, Borrione, Paolo, Romanelli, Francesco, and Di Luigi, Luigi

Subjects
EXERCISE, SCIENTIFIC literature, NUTRITION, LONGEVITY
Abstract

Background: Sarcopenia is a pathophysiological condition diffused in elderly people; it represents a social issue due to the longer life expectancy and the growing aging population. It affects negatively quality of life and it represents a risk factor for other pathologies, such as diabetes, cardiovascular disease, and obesity. No silver bullet exists to hinder sarcopenia, but it may be counteracted by physical exercise, nutrition, and a proper endocrine milieu. Indeed, we aim to analyze the scientific literature to give to clinician effective advices to counteract sarcopenia. Main text: Physical exercise, proper nutrition, optimized hormonal homeostasis represent the three pillars to fight sarcopenia. Physical exercise represents the most effective remedy to face sarcopenia, in particular if it is combined with a proper diet and with an adequate endocrine milieu. Consistency in training, adequate daily protein intake and eugonadism seems to be the keys to fight sarcopenia. The combination of these three pillars might act synergistically.Conclusions: Optimization of these factors may increase their efficiency; however, scientific data may be sometimes confusing so far. Therefore, we aim to give practical advices to clinician to identify and to highlight the most important aspects in each of these three factors that should be addressed. [ABSTRACT FROM AUTHOR]

Published
2019
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