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Objective: This paper discusses the feasibility of using mineral element fingerprinting technology of to identify tobacco producing areas, screens out the effective indicators, and constructs the discrimination model of tobacco producing areas. Methods: The contents of 20 mineral elements in tobacco leaves from 11 producing areas were simultaneously determined by ICP-MS, and the data were analyzed by variance analysis, cluster analysis, principal component analysis and discriminant analysis. Results: The contents of 16 elements were significantly different among producing areas, and principal component analysis resulted in 6 principal components, with the cumulative variance contribution of 89.253%. Using linear discriminant analysis, four elementsmineral, K, Mn, Se and Ba, were screened as the effective indicators to discriminate the geographical origin of tobacco leaves. The established discriminant model could accurately distinguish tobacco leaf samples from different tobacco producing areas. Conclusion: Significant differences were showed in the contents of mineral elements in tobacco leaves from 11 producing areas. Four elements, K, Mn, Se and Ba, are important indicators for distinguishing tobacco producing areas. Mineral element fingerprinting technology can be used for distinguishing tobacco producing areas.

With 12 varieties of flue-cured tobacco as a template, the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized. Multiplex PCR fingerprints and identification routes of 12 flue-cured tobacco varieties were established by multiplex PCR. The results showed that multiplex PCR had the advantages of high efficiency, high speed, time saving and low cost compared with ordinary PCR. Six pairs of SCAR primers had good stability and specific band specificity, which could distinguish 12 flue-cured tobacco varieties accurately. [ABSTRACT FROM AUTHOR]

Using the single factor and orthogonal experiments with the flue-cured tobacco varieties of zhongyan 100 as raw material and two SCAR primers to study the multiple PCR reaction system in the inter action between the main ingredients of flue-cured tobacco leaves, the best suitable multiple PCR reaction system was established for roast tobacco including DNA template 45 ng, Mg2+ concentration 2.50 mmol/L, dNTP concentration 0.40 mmol/L and Taq enzyme dosage 4.00 U on the 20 µL system. The stripe of the system is clear and it can shorten the identification time, laying a foundation for using multiple PCR technology to identify flue-cured tobacco varieties. [ABSTRACT FROM AUTHOR]