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2 results on '"SUBSP. PARATUBERCULOSIS"'

1. Histopathological and Serological Studies on Paratuberculosis in Cattle and Buffaloes

Author

Arbab Sikandar*, AH Cheema1, M Younus2, A Aslam1, MA Zaman2 and T Rehman

Subjects
Buffalo, Cattle, ELISA, Histopathology, Mycobacterium avium, subsp. Paratuberculosis, Paratuberculosis, Animal culture, SF1-1100, Veterinary medicine, SF600-1100
Abstract

Paratuberculosis (Johne’s diseases) is responsible for massive economic losses to dairy industry, both in the industrially advanced as well as in the developing countries. To detect its occurrence in cattle and buffaloes locally, blood and tissue samples from clinically weak and grossly suspected slaughtered animals were collected from two abattoirs of Jhang, municipal area, Pakistan. Acid-fast smear staining, gross/histopathology and indirect ELISA were done for the detection of Mycobacterium avium subsp. paratuberculosis (MAP). Total 134 samples illustrating gross pathological lesions were collected, only 11.19% (cattle: 6.67%, buffaloes: 12.5%) showed acid fast bacilli through smear staining and were taken as confirmed cases. Thickening of intestines alone was not a reliable indicator of Johne’s disease. Tissue sections from intestines and mesenteric lymph nodes from these acid fast positive animals were stained with hematoxylin & eosin (H&E) and Ziehl Neelsen (ZN) methods. Sum of (15/134) impression smear staining as well as (15/15) tissue sections of the intestines were found ZN positive, and only 6.7% of impression smears and 100% of tissue sections of mesenteric lymph nodes showed acid fast bacilli. Through ELISA, two cattle and five buffaloes (07/134) gave positive optical densities, while one cattle and seven buffaloes (08/134) were judged as doubtful. It is concluded that infection of MAP can be identified by histopathology and ELISA. The present study was the first record of paratuberculosis among the dairy animals slaughtered at Jhang abattoirs. The objective was to compare different methods for the diagnosis of Johne’s disease.

Published
2012

2. Fecal Detection of Mycobacterium avium Paratuberculosis Using the IS900 DNA Sequence in Crohn’s Disease and Ulcerative Colitis Patients and Healthy Subjects

Author

Alessandra Caponi, Giulia Roda, Giampaolo Ugolini, Lucia Castellani, Franco Bazzoli, Alessandra Munarini, Lorenzo Fuccio, Michele Scagliarini, Margherita Marocchi, Andrea Belluzzi, Anna Tuci, Alessandro Sartini, F. Tonon, Giancarlo Rosati, Tuci, A., Tonon, F., Castellani, L., Sartini, A., Roda, G., Marocchi, M., Caponi, A., Munarini, A., Rosati, G, Ugolini, G., Fuccio, L., Scagliarini, M, Bazzoli, F., and Belluzzi, A.

Subjects
Adult, DNA, Bacterial, Male, MYCOBACTERIUM AVIUM, Adolescent, Physiology, Population, Asymptomatic, Pathogenesis, Feces, Young Adult, Crohn Disease, medicine, Humans, education, Aged, Human feces, Crohn's disease, education.field_of_study, Chi-Square Distribution, Base Sequence, business.industry, Gastroenterology, Reproducibility of Results, Middle Aged, medicine.disease, Ulcerative colitis, DNA extraction, Mycobacterium avium subsp. paratuberculosis, SUBSP. PARATUBERCULOSIS, PCR, Case-Control Studies, Data Interpretation, Statistical, Immunology, Colitis, Ulcerative, Female, medicine.symptom, business, CROHN’S DISEASE, IS900
Abstract

BACKGROUND AND AIM: Despite the increasing evidence of MAP/DNA isolation in Crohn's disease (CD), its potential pathogenetic role remains unclear. To further clarify the possible relationship between MAP and CD, we investigated the presence of IS900 DNA fragment in feces from Crohn's disease and ulcerative colitis (UC) patients and from healthy controls (HC). METHODS: Stool samples were collected from 31 CD, 20 UC, and 23 HC and stored at -20°C in 200-mg aliquots. DNA was extracted. MAP presence was detected with a specific PCR amplifying a 409-bp fragment from IS900. The specificity of PCR for IS900 was confirmed sequencing three positive products. Statistical analysis was performed using the Chi-square test. RESULTS: Twenty-one of 31 CD (68%), 13 of 20 UC (65%) and 11 of 23 HC (48%) were MAP-positive (CD vs. HC: p = ns; UC vs. HC: p = ns). With the limits of a small sample size, the IS900-positive percentage in CD and UC was higher than HC, although the difference was not statistically significant. CONCLUSIONS: The possibility to track the MAP presence in human feces represents a new approach to the "MAP hypothesis". Detection of MAP DNA in feces is very common, reaching very high prevalence both in CD and in UC and even in HC. Our findings seem consistent with a high prevalence of MAP asymptomatic infection among the general population and so the possible involvement of MAP in CD pathogenesis could be linked to a specific immune defective response.

Published
2011

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