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Covid-19 posed little danger to children. Nevertheless, the South African government imposed lockdown measures that impeded children's education, play and food. Schools were closed, feeding schemes were halted and organised sports were banned. In this study of South African children's experience during the 2020–22 pandemic, we use the capabilities approach, particularly Nussbaum's ten capabilities, to assess how the lockdown measures affected their development. The one-dimensional nature of the government response was evident in its focus on physical health and safeguarding adults and the health system, at the expense of children's well-being. Children were masked and their voices were not heard. Despite being far less at risk of illness or death than adults, children and young people were disproportionately affected by government regulations. Children from low-income environments were particularly severely affected. [ABSTRACT FROM AUTHOR]

Violence remains a persistent challenge in South African schools, prompting investigations into underlying risk factors and mitigation strategies. However, an under-explored aspect of this violence is the potential link between the consumption of Internet porn via cellphones among girls and boys, and girls' risks to sexual violence inside the classroom. To address this gap, we used focus group discussions with 14–17-year-old South African girls to examine their experiences of porn access via cellphones and their accounts of sexual violence at school. The study illuminates the nuanced ways in which the girls experience risks and express agency. First, the girls illustrate a link between sexual harassment and boys accessing porn on their cellphones during class. Second, the findings show how girls negotiate their sexual agency and safety through contesting sexual violence. Third, there are conflicting views about porn: while some girls admitted liking and viewing porn, others objected to it as harmful and degrading. Finally, this study offers crucial insights into strategies to create safer school environments and gender equality by drawing attention to the intersections between cellphone porn consumption, sexuality, and girls' negotiations of sexual agency amidst sexual risk in the classroom. [ABSTRACT FROM AUTHOR]

Our ethnographic fieldwork with Congolese and Zimbabwean economic migrants, asylum seekers, refugees and educational migrants reveals a complex narrative of exclusion and inclusion in South Africa. Frontier Africans – South African citizens and African transmigrants alike – initiate and maintain connections that create a vexing and entangled narrative of hard and soft borders. A deeper consideration of how South African citizens and African transmigrants subvert and transcend state-centric categorisation of citizen and migrant other, through amity, conviviality and mutuality – a process of humaning – harasses the alignment of political categorisation with analytical elucidation. [ABSTRACT FROM AUTHOR]

Objective: To assess the unmet needs of South Africans with axial spondyloarthritis (axSpA) focusing on the patient journey, functional disability, and health-related quality of life. Methods: One hundred forty-six South African axSpA patients completed the International Map of Axial Spondyloarthritis (IMAS) online survey. Patient journey, functional disability, activities of daily living, and psychological stress were analyzed in relation to socio-demographic characteristics, disease activity, diagnostic delay, extra-musculoskeletal manifestations, and drug therapy. Results: Majority were female (82.2%) and Caucasian (89.7%) and the mean age of participants, age at onset of initial symptoms, and diagnostic delay were 44.7, 26.7, and 10.8 years, respectively. Participants reported a mean of 3.4 visits to healthcare professionals prior to a definitive diagnosis of axSpA, mostly made by rheumatologists (77.9%). Active disease (BASDAI ≥ 4) was reported by 87%, 69.9% suffered from psychological distress (general health questionnaire-12 score ≥ 3), and more than two-thirds suffered functional limitations in daily, personal, and social activities. Multivariable logistic analysis showed that active disease was more common in females [OR (95% CI) = 4.3 (1.2–15.2)] and was associated with greater functional limitation [OR (95% CI) = 1.1 (1.0–1.2)]. Conclusion: Of all the regions assessed in the IMAS (n = 5557 participants, 27 countries), South Africans reported the longest delay in diagnosis. The South African patient journey depicts a process burdened with diagnostic challenges and delays, coupled with patients experiencing significant personal and social limitations. These results emphasize the urgent need to establish local diagnostic and treatment guidelines for axSpA in South Africa, to reduce diagnostic delay, and to control disease activity associated with functional limitation in axSpA. Key Points • Axial spondyloarthritis (axSpA) in South Africans is associated with significant limitations in physical, mental, and social functioning. • First study to describe the unmet needs of South African patients with axSpA. [ABSTRACT FROM AUTHOR]

Continual re-evaluation of standards for forensic anthropological analyses are necessary, particularly as new methods are explored or as populations change. Indian South Africans are not a new addition to the South African population; however, a paucity of skeletal material is available for analysis from medical school collections, which has resulted in a lack of information on the sexual dimorphism in the crania. For comparable data, computed tomography scans of modern Black, Coloured and White South Africans were included in addition to Indian South Africans. Four cranial morphoscopic traits, were assessed on 408 modern South Africans (equal sex and population distribution). Frequencies, Chi-squared tests, binary logistic regression and random forest modelling were used to assess the data. Males were more robust than females for all populations, while White South African males were the most robust, and Black South African females were the most gracile. Population differences were noted among most groups for at least two variables, necessitating the creation of populations-specific binary logistic regression equations. Only White and Coloured South Africans were not significantly different. Indian South Africans obtained the highest correct classifications for binary logistic regression (94.1%) and random forest modelling (95.7%) and Coloured South Africans had the lowest correct classifications (88.8% and 88.0%, respectively). This study provides a description of the patterns of sexual dimorphism in four cranial morphoscopic traits in the current South African population, as well as binary logistic regression functions for sex estimation of Black, Coloured, Indian and White South Africans. [ABSTRACT FROM AUTHOR]

Critical thinking can be promoted through narratives, puzzles, and riddles. Literature has indicated a need to reactivate African narratives to preserve African legacies for future generations. This study argues that African riddles, puzzles, and narratives should be used in African-themed video games to promote critical thinking and awareness of African culture. It emphasizes the importance of African-centered puzzles as a culturally relevant pedagogy that fosters students' understanding of African cultural values. The study used a participatory culture framework to bring students together to develop an African-themed video game. The game, called Nolwazi, was investigated ethnographically to highlight critical aspects. The study presents the findings of the investigation and analysis. It concludes with a discussion of the importance of using African riddles, puzzles, and narratives in video games. Plain language summary: Critical thinking promoted through African Themed Video Games The study explores the utilization of the rich African narratives, puzzles and riddles as tools in teaching students through video games. This study focuses on video games developed by South African students and how the video games have helped to create cultural awareness and foster critical thinking. [ABSTRACT FROM AUTHOR]

Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder (NDD) characterized by significant impairments in social, communicative, and behavioral abilities. However, only a limited number of studies address the genetic basis of ASD in the African population. This study aims to document the genes associated with ASD in Africa and the techniques used to identify them. Additionally, genes identified elsewhere but not yet in Africa are also noted. Methods: Online databases such as Wiley Online Library, PubMed, and Africa Journal Online were used. The review was conducted using the keyword related to genetic and genomic ASD study in the African population. Result: In this scoping review, 40 genetic studies on ASD in Africa were reviewed. The Egyptian and South African populations were the most studied, with 25 and 5 studies, respectively. Countries with fewer studies included Tunisia (4), East African countries (3), Libya (1), Nigeria (1), and Morocco (1). Some 61 genes responsible for ASD were identified in the African population: 26 were identified using a polymerase chain reaction (PCR)-based method, 22 were identified using sequencing technologies, and 12 genes and one de novo chromosomal aberration were identified through other techniques. No African study identified any ASD gene with genome-wide association studies (GWAS). Notably, at least 20 ASD risk genes reported in non-African countries were yet to be confirmed in Africa's population. Conclusion: There are insufficient genetic studies on ASD in the African population, with sample size being a major limitation in most genetic association studies, leading to inconclusive results. Thus, there is a need to conduct more studies with large sample sizes to identify other genes associated with ASD in Africa's population using high-throughput sequencing technology. [ABSTRACT FROM AUTHOR]

Parenting has been found to be highly contextually and culturally determined and there have been calls to research parenting within culture as it is lived. Due to changing social and economic factors, middle-class South African mothers face unique challenges in relation to the navigation of culture and class in child-rearing. Foregrounding the complexity of acculturation, this paper uses social constructionist theory in the analysis of maternal narratives and responses to video-recordings of their interaction with their infants, of a group of middle-class South African mothers from various cultural and racial groups, with an aim to understanding how mothering is changing amongst middle-class South African mothers. The findings suggest that acculturation is complex and influenced by a combination of socioeconomic status, geographical location, contact with other cultural groups and personal emotional experiences of having been parented within a particular culture. [ABSTRACT FROM AUTHOR]

We investigated the incidence and duration of morbidity symptoms among infants aged 6 to 9 months from a low socioeconomic community receiving one egg daily for 6 months. This was a secondary outcome of a randomised controlled trial (RCT) of 500 infants conducted in Jouberton, South Africa. The primary outcome was linear growth. Morbidity data were collected weekly using a symptoms diary and qualitative data with focus group discussions at the endpoint. Ethical approval was obtained from the North-West University Health Research Ethics Committee. The intervention group had a ~ 5% higher incidence of gastrointestinal morbidity (17.0%) compared to the control group (11.9%). Gastrointestinal morbidity without fever tended to be 1.4 times higher in the intervention group (OR: 1.43, 95% CI: 1.03, 1.93; P = 0.058) and tended to be 4 times higher with fever (OR: 4.07, 95% CI: 0.86, 19.23; P = 0.077). The duration of total gastrointestinal and respiratory morbidity was 1.5 days longer in the intervention group (β: 1.491; 95% CI 0.064, 2.918; P = 0.041). Complementary feeding with eggs may have contributed towards an increased risk for gastrointestinal morbidity. [ABSTRACT FROM AUTHOR]

Engineers’ contribution to society tends to be located in rational, technical constructs devoid of social and political interests. This paradigm presents a challenge to engineering educators tasked with undergraduate engineering curriculum content that links to society in some way. This article reports on a study undertaken by engineering educators who were motivated to understand the role of the social and political on the work of engineers. It explores how engineers conceived of their role during apartheid, an oppressive political regime for the majority of South Africans that officially ended in 1994. Interviews were conducted with engineers and academic staff to examine how participants’ navigated engineering practice and academic work during that period. The thematic findings allow for a conceptualisation of the apartheid ‘system’ they encountered, depicts how they saw their role in this system and explores the underlying reasons for why they behaved in the ways that they did. [ABSTRACT FROM AUTHOR]

AbstractThis qualitative research investigates the career experiences of 14 female musicians in the city of Gqeberha in the Eastern Cape province of South Africa. Findings accord with research conducted in other countries, and like their international counterparts, these musicians find themselves in a complex and precarious industry. They develop their identity or brand, build networks, and pursue lifelong learning while working in non-music fields to sustain their employability. The findings also indicate that a music qualification is not a prerequisite for employment except in the field of music education, and that such formal qualifications are associated with greater job security in this field. These findings offer new insights and a nuanced understanding of how a specific group of music creatives navigate protean careers. [ABSTRACT FROM AUTHOR]

In poor-performing, resource-constrained education systems, such as South Africa, solutions to improve academic achievement have tended to focus on resources and cognitive factors. An area that requires more attention is the importance of non-cognitive, psychosocial factors and their relationship with academic performance. These factors are strongly associated with behaviour, playing a role in either facilitating or impeding the learning process. Understanding attitudes is therefore critical in interpreting achievement results with a view to improving them. This article used data from the 2019 Trends in International Mathematics and Science Study administered to 20,829 South African students in Grade 9 to investigate the confidence these students have in their mathematics ability (their self-efficacy) and the relationship with achievement. Linear regression analysis was employed to determine: (1) the relationship between Grade 9 students’ self-efficacy and mathematics achievement in South Africa; and (2) the contextual factors that were associated with the mathematics self-efficacy of the students. The results indicate a positive correlation between students’ self-efficacy and their performance in mathematics. In addition, good teaching practices and parental involvement in school activities were associated with higher levels of students’ mathematics self-efficacy. Being female was associated with lower levels of self-efficacy. These findings have important implications for shaping educational policy and practice in the resource constrained context of South Africa, to enhance students’ self-efficacy and performance in mathematics. [ABSTRACT FROM AUTHOR]

PurposeMethodsResultsConclusionAlthough fragility hip fractures (HF) in the South African (SA) population are among the lowest worldwide, the incidence of HF is expected to more than double over the next few decades. Little is known about the contributors to increased hip fracture risk, including low bone mineral density (BMD), in our unique population. In addition, the ability of the recently calibrated SA Fracture Risk Assessment Tool (FRAX) to identify high fracture risk in the SA population accurately has not been validated.A retrospective, descriptive, cohort study of SA postmenopausal women and men ≥ 50 years who presented with fragility HFs was conducted. The ability of clinical risk factors (CRFs) and BMD measured by dual-energy X-ray absorptiometry (DXA), as well as calculated FRAX probability scores, to identify the known high fracture risk in SA patients were evaluated. The SA FRAX tool was used, and a high fracture risk defined if the United States (US) fixed thresholds for major osteoporotic (MOF) and/or HF risk were exceeded (≥ 20% and ≥ 3% over 10 years respectively).A total of 163 patients were included. The most useful predictive CRFs were age and gender, recreational toxins in men, and a history of falls. Most were females (71%), who were older than males. DXA-BMD and FRAX-HF calculations best identified the known high fracture risk in the study cohort. FRAX-MOF calculations performed poorly.Fracture risk assessment tools did not identify the known high fracture risk in all of the elderly study cohort with HF. Clinicians must continue to appreciate the important role of a good clinical assessment to ensure optimal fracture risk prediction. [ABSTRACT FROM AUTHOR]

Beliefs about the malleability of intellectual ability—mindsets—shape achievement. Recent evidence suggests that even young children hold such mindsets; yet, no reliable and valid instruments exist for measuring individual differences in young children's mindsets. Given the potential relevance of mindsets to children's achievement-related behavior and learning, we developed and tested the psychometric properties of the Growth Mindset Scale for Children (GM-C). Among other psychometric properties, we assessed this instrument's (a) factor structure, (b) measurement invariance, (c) internal consistency, (d) temporal stability (test-retest reliability), (e) concurrent validity, and (f) cross-cultural robustness in samples of US children (Study 1; N = 220; ages 4 through 6; 50% girls; 39% White) and South African children (Study 2; predominantly grades 4 and 5; N = 331; 54% girls; 100% non-White). The GM-C scale exhibited four factors, representing beliefs about the instability of low ability, the malleability of low ability, the instability of high ability, and the malleability of high ability. The GM-C scale also demonstrated invariance across age, acceptable internal consistency (αs between.70 to.90), and moderate temporal stability over approximately one month (rs between.38 to.72). Concurrent validity was supported by significant relations between children's scores on the subscales about low ability and their goal orientations (Studies 1 and 2), challenge-seeking behavior, and achievement in math and English (Study 2). These findings suggest that the GM-C scale is a promising tool for measuring mindsets in young children. We offer practical recommendations for using this new scale and discuss theoretical implications. [ABSTRACT FROM AUTHOR]

Background/Objectives: Tobacco smoking is a cause of premature death and illness globally. This study examined the prevalence and factors associated with tobacco smoking among South African adults according to sex, education, and employment status among socio-demographic subgroups. Methods: Data were obtained from the 2021 South African Global Adult Tobacco Survey (a nationally representative household survey using a multi-stage stratified cluster random sampling design). The 6311 participants were aged 15+ years. Descriptive statistics, chi-square tests, and multiple logistic regression analysis were used to investigate relationships between current smoking and socio-demographic variables. Results: Smoking prevalence was 25.8% (n = 1573) (41.2% among men, 11.5% among women; 29.9% among working individuals, 23.1% among non-working individuals). The multiple logistic regression results showed that males aged 45–64 were twice as likely to be currently smoking than males aged 15–24. Coloured (of mixed race) males were twice as likely to smoke as Black African males. Males with secondary/post-secondary education were less likely to smoke compared to males with no education. Coloured females were seven times and White females were almost five times more likely to smoke than Black African females. Females with post-secondary education were less likely to smoke than those with no formal education. Females earning an income were more likely to smoke compared to those with no income. Non-working participants aged 25–44 and 45–64 were more likely to smoke compared to those aged 15–24 years. Working and non-working Coloured and working White individuals were three times more likely to smoke than working or non-working Black Africans. Conclusion: Groups with higher identified smoking prevalence may indicate where smoking cessation interventions should be targeted to reduce national smoking prevalence. [ABSTRACT FROM AUTHOR]

Background COVID-19 vaccine effectiveness (VE) studies leveraging systematic surveillance in sub-Saharan Africa are limited. We assessed the effectiveness of two vaccines (Pfizer BNT162b2 and Johnson & Johnson Ad26.COV2.S) against SARS-CoV-2-associated hospitalization in South African adults aged ≥18 years. Methods We conducted a test-negative case-control study using pneumonia surveillance data in South Africa. Inpatients with physician-diagnosed lower respiratory tract infection or suspected COVID-19, testing SARS-CoV-2 positive or negative from June 2021–March 2022, were cases or controls, respectively. Fully vaccinated individuals received one Ad26.COV2.S dose or two BNT162b2 doses ≥14-days before enrollment. VE was estimated using multivariable logistic regression for Delta- and Omicron BA.1/BA.2-predominant periods, stratified by age and HIV status. Results The study included 925 cases and 1890 controls; 38 (4%) cases and 186 (10%) controls were fully vaccinated with BNT162b2, and 30 (3%) cases and 94 (5%) controls with Ad26.COV2.S. The vaccine effectiveness of BNT162b2 against SARS-CoV-2-associated hospitalization over Delta and Omicron BA.1/BA.2 periods was 91% (95% CI: 52%, 98%) and 33% (-16%, 86%), respectively. The vaccine effectiveness of Ad26.COV2.S against hospitalization over Delta and Omicron BA.1/BA.2 periods was 72% (-36% ,94%), and -19% (-130%, 39%), respectively. The vaccine effectiveness of BNT162b2 against hospitalization over the Delta period was 94% (50%, 99%) and 89% (27%, 98%) among adults aged ≥60 years and HIV-uninfected, respectively. Conclusions The BNT162b2 vaccine was effective against SARS-CoV-2-associated hospitalization during the Delta period for adults aged ≥18 years, ≥60 years and those HIV-uninfected. VE for Ad26.COV2.S was inconclusive, potentially due to limited sample size or residual confounding. These findings highlight the utility of sentinel surveillance for estimating VE. [ABSTRACT FROM AUTHOR]

Biological mechanisms underlying anxiety proneness (AP), the tendency to react fearfully to stressors due to the belief that experiencing anxiety has harmful consequences, remain unclear. Epigenetic mechanisms, such as microRNAs (small, non‐coding RNAs 19–20 nucleotides long), may be contributory. This study investigated AP‐associated differences in microRNA expression among South African adolescents with variable exposure to childhood trauma (CT). AP was assessed using a composite score reflecting trait anxiety and anxiety sensitivity, while CT exposure was assessed with the Childhood Trauma Questionnaire. High‐quality total RNA (n = 88) extracted from whole blood underwent microRNA‐sequencing. Differential microRNA expression analysis was conducted with DESeq2 in R, messenger RNA target prediction analysis was performed using TargetScan and DIANA‐microT, and the DIANA mirPATH tool was used for KEGG pathway analysis. The majority of participants were female (75.86%) with an average age of 15 (±1.19) years. MicroRNA expression analysis identified upregulation of hsa‐miR‐28‐5p and downregulation of hsa‐miR‐502‐3p and hsa‐miR‐500a‐3p in high‐AP individuals, irrespective of CT. Four KEGG pathways, each with ≥10% of their constituent genes predicted to be targets of the differentially expressed microRNAs, were identified and were enriched for genes involved in calcineurin and glutamate signalling. These findings suggest that epigenetically mediated effects on neuronal function contribute to the molecular aetiology of AP. [ABSTRACT FROM AUTHOR]

Background:Aim:Methods:Results:Conclusion:Breastfeeding is widely acknowledged as the optimal method of infant feeding. However, the percentage of South African mothers who exclusively breastfeed remains alarmingly low. Healthcare workers (HCWs) are pivotal in promoting breastfeeding; however, a deficiency in their knowledge, negative attitudes, and unfavourable practices can impede the promotion and support of breastfeeding.To assess the knowledge, attitudes, and support practices related to breastfeeding promotion of HCWs in the Motheo district, Free State province, compared with the 2016 WHO Guideline updates on HIV and infant feeding and 2018 Mother-Baby Friendly initiative guidelines.A quantitative analytical cross-sectional study involving paediatricians, obstetricians, general practitioners (GPs), and midwives was conducted using a self-administered questionnaire.Over half of the HCWs (59%) were able to define exclusive breastfeeding (EBF), with a significant majority of GPs (76.1%) and midwives (78.6%) recommending EBF up to six months, compared with only 10% of paediatricians and 25% of obstetricians (p < 0.001). Some 11.1% of the total group of HCWs reported that breastfeeding is contraindicated for HIV-infected mothers, while 15.4% were unsure of the recommendation for breastfeeding among HIV-infected mothers. A higher proportion of midwives, compared with other HCWs, felt highly confident in providing breastfeeding advice (p < 0.001). Additionally, more than half (56%) of HCWs had not completed the 20-hour WHO Lactation Management Training, and 55% believed their prior breastfeeding training was not sufficient to support and educate breastfeeding mothers.Regular breastfeeding training of HCWs on the latest breastfeeding guidelines is necessary to equip HCWs with the knowledge and competence to successfully promote, protect, and support breastfeeding. [ABSTRACT FROM AUTHOR]

Aims: This study aims to investigate miR‐486‐5p and miR‐novel‐chr1_40444 expressions in dysglycemic individuals. Validating RNA‐sequencing findings in a larger sample via reverse transcription qPCR (RT‐qPCR), we aim to address global diagnostic and screening limitations, using an African cohort as an example. Materials and Methods: This cross‐sectional study involved 1,271 individuals [normoglycemic (n = 974), prediabetic (n = 206), screen‐detected type 2 diabetes (n = 91)] from the ongoing Vascular and Metabolic Health (VMH) study in Cape Town, South Africa. Whole blood miRNA expression was assessed using TaqMan‐based RT‐qPCR, with data normalized to an endogenous control (miR‐16‐5p). Results: Significant underexpression was observed in prediabetes vs normoglycemia for miR‐486‐5p (P = 0.038), whilst both miRNAs demonstrated significant upregulation in screen‐detected type 2 diabetes vs normoglycemia (miR‐486‐5p, P = 0.009; miR‐novel‐chr1_40444, P < 0.001), and screen‐detected type 2 diabetes in comparison with prediabetes (miR‐486‐5p, P < 0.001; miR‐novel‐chr1_40444, P < 0.001). Multivariable regression analyses revealed pronounced interrelations between miR‐novel‐chr1_40444 and screen‐detected type 2 diabetes in unadjusted and adjusted models (Model 1: P < 0.001, Model 2: P < 0.001, Model 3: P = 0.030). Moreover, receiver operating characteristic (ROC) curves revealed significantly enhanced diagnostic capabilities for screen‐detected type 2 diabetes vs either normoglycemia (AUC = 0.971, P < 0.001), non‐diabetes (AUC = 0.959, P < 0.001), or prediabetes (AUC = 0.902, P < 0.001) when combining the miRNAs with 2 h postprandial glucose. Conclusions: This study demonstrated the enhanced power of incorporating miRNAs with traditional markers in distinguishing screen‐detected type 2 diabetes, warranting further investigations on their unique role in the development of type 2 diabetes. [ABSTRACT FROM AUTHOR]

Purpose: The insula, a cortical structure buried deep within the sylvian fissure, has long posed a surgical challenge. Comprehensive knowledge of the insular anatomy is therefore integral to preoperative planning and safe interventional procedures. Since magnetic resonance imaging (MRI) is a favoured modality for the identification of cerebral structures, this study aimed to investigate the morphology and morphometry of the insula in a South African population, using MRI scans. Methods: One-hundred MRI studies of insulae (n = 200 hemispheres) were retrospectively analysed for morphological features and morphometric parameters. Results: The insulae were predominantly trapezoidal in shape (Laterality: Left: 82%; Right: 78%; Sex: Male: 84%, Female: 76%). The central insular sulcus was almost always "well seen" (Laterality: Left: 97%; Right: 99%; Sex: Male: 99%, Female: 97%). The middle short insular gyrus (MSG) was most variable in visibility, especially when compared across the sexes (p = 0.004). Insular gyri widths were comparable in both cerebral hemispheres; the posterior long gyrus (PLG) presented with the smallest mean widths. Anterior lobule (AL) widths were larger than those of the posterior lobule (PL). Widths of the insular gyri and lobules were generally larger in males than in females. The MSG and PLG widths in the left hemisphere, AL width in the right hemisphere, and the PL width in both hemispheres were significantly larger in males than in females (p = 0.001; p = 0.005; p = 0.041; p = 0.001, p = 0.015, respectively). Conclusion: MRI scans may be used to accurately interpret insular anatomy. The data obtained may aid neurosurgeons to perform safe insula-related surgical procedures. [ABSTRACT FROM AUTHOR]

• The pompom weed is likely pollinated by Apis mellifera and Hypolimnas misippus. • Gametophytic apomixis with autonomous endosperm development in triploids. • The co-occurrence of apomixis and polyploidy may be facilitating its invasiveness. • There is high pollen grain viability despite apomictic reproduction. • Low genetic diversity within and among the sampled triploid populations. Polyploidy and apomixis enable successful plant invasions into new regions. Campuloclinium macrocephalum (pompom weed) is a successful alien invasive species in South Africa that has been shown to comprise multiple ploidy levels. Despite the identification of triploid and tetraploid cytotypes in South African populations, the primary reproductive strategy was unknown. We investigated the reproductive strategy used by triploid populations of the pompom weed. We assessed whether insects contributed to pollen transfer using pollinator exclusion experiments, estimated genetic diversity, evaluated pollen tube growth in the styles, and estimated male fertility using pollen grain size and viability. We found evidence that strongly suggests that the pompom weed can reproduce via gametophytic apomixis with autonomous endosperm development. Pollinator exclusion experiments showed that while insects may positively influence reproductive success, the pompom weed can set lower quantities of seed in the absence of pollinators and that overall seed viability did not differ between open and excluded pollinator treatments. Further, while individuals are capable of uniparental reproduction, seed set appears to be independent of fertilization because we did not observe pollen tubes growing down the styles and/or reaching the ovules, despite high pollen viability. The sampled triploid populations also showed low genetic diversity, which may be a result low recombination frequencies. The low genetic variation should result in all of the sampled populations being equally susceptible to the biocontrol agents; however, this is not being observed in the sampled populations. Only one out of the four sampled populations showed a significant reduction in seed set and germination percentages due to the damage from Puccinia eupatorii (rust fungus) and Liothrips tractabilis (thrips). Nevertheless, the combination of an apomictic reproductive strategy and polyploidy is likely contributing to making pompom weed a formidable invader in South Africa. [ABSTRACT FROM AUTHOR]

Background: Temporomandibular disorders (TMD) affect a significant portion of the population, with profound psychological, behavioral, and social repercussions. Recent investigations have explored the genetic basis underlying pain perception in individuals with TMD, aiming to elucidate the role of specific genetic factors in modulating the condition. Notably, genetic variations have been implicated in the pathogenesis of TMD, particularly genes involved in pain perception pathways. One of the primary candidates is the Catechol-O-Methyltransferase (COMT) gene, which plays a crucial role in the catecholaminergic system and has been associated with the regulation of nociceptive processes. This study seeks to investigate the correlation between COMT gene activity and pain perception among South African patients diagnosed with varying forms of TMD. Methodology: In this study, a total of 196 participants were enrolled, comprising 97 patients diagnosed with TMD and 99 control participants. The control group was meticulously matched with the TMD group for age, gender, and ethnicity. Data collection involved clinical and radiological investigations, and saliva sampling. The English version of the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) Axis I was utilized to evaluate all TMD participants, focusing on standard diagnostic measures based on clinical signs and symptoms of TMD, which primarily describe common physical manifestations of the disorder. Genomic DNA was extracted from saliva samples, enabling the analysis of single-nucleotide polymorphisms (SNPs) in the COMT gene, specifically targeting polymorphisms rs165774, rs9332377, rs6269, rs4646310, rs165656, and rs4680. Results: The current study demonstrated a pronounced gender disparity, with 80.41% of the participants being female and 19.59% male, suggesting that women in South Africa either exhibit a higher susceptibility to TMD or are more likely to seek treatment for the condition compared to men. The highest prevalence of TMD was observed in the white population (58.76%). Additionally, over 65% of TMD patients were diagnosed with at least two Axis I diagnoses, a figure that increased to 89% for those diagnosed with three Axis I diagnoses. The findings further indicated significant associations between several single-nucleotide polymorphisms (SNPs) in the Catechol-O-Methyltransferase (COMT) gene—specifically rs165656, rs9332377, rs4646310, rs6269, and rs165774—and both TMD and TMD-related pain. Myofascial pain with referral and myalgia showed a strong association with the COMT SNPs rs9332377 and rs4646310. Furthermore, COMT SNP rs4646310 was also associated with disability related to TMD. Conclusions: This study substantiates the hypothesis that pain is prevalent in a considerable proportion of patients affected by TMD. Furthermore, the findings reveal a significant association between COMT gene activity and pain perception in South African patients diagnosed with TMD. [ABSTRACT FROM AUTHOR]

Fusarium circinatum is a globally important fungal pathogen that causes pitch canker on a wide range of Pinus species. In this study, we considered mycelial growth in culture and aggressiveness of a South African population of F. circinatum isolates. The specific aim was to determine how these phenotypes are correlated with one another and other isolate characteristics, including mating type, host species and geographic origin of the isolates. The study involved the selection of 102 isolates of F. circinatum from five provinces of South Africa based on genotype, mating type and original host from which they were isolated, after which pathogenicity tests were carried out on Pinus patula seedlings. Mycelial growth in culture was also determined for all the isolates at three different temperatures. In the pathogenicity tests, most of the isolates produced significant lesions. While seven had low levels of aggressiveness, other isolates tested were as or more aggressive than a standard set of isolates routinely used for resistance/ tolerance screening. Most F. circinatum isolates grew best at 25 °C, followed by growth at 20 °C and 30 °C. A notable correlation between aggressiveness and the geographical origin was identified. Moreover, the study highlighted a moderately positive relationship between the growth rate of F. circinatum isolates and their reported aggressiveness. The extensive data gathered on the aggressiveness of F. circinatum will offer a broad range of isolates for future comparative studies using other genotypes of F. circinatum and will also aid in the consideration of F. circinatum isolates used for tolerance screening studies. [ABSTRACT FROM AUTHOR]

Treatment of HIV in South Africa is informed by national guidelines, which are regularly updated. Ongoing training of healthcare workers is vital for optimal patient care, but is challenging, due to human, financial and infrastructural constraints. There is a need for innovative solutions to meet training and support needs. With 93.2% of South African adults using WhatsApp, it is an ideal platform for training, even in the most remote clinics. The primary aim of the study is to design, test and evaluate the effect of WhatsApp group-based HIV training on nurses' and community health workers' knowledge. The secondary aims are to assess uptake, acceptability, and feasibility of the intervention; and to explore and describe the changes in prescribing, comparing the intervention group to the control group. A pragmatic, mixed-methods, parallel-group cluster-randomised study design will be used. This paper details the design – and rationale behind it – of the study. A critical realist stance was chosen, with outcomes measured using questionnaires, pre- and post-intervention; WhatsApp group interaction; focus groups; and folder reviews. Inferential analysis will be conducted using Stata, descriptive analysis using Excel™ and qualitative data using template analysis with NVivo™. Rigor of the study will be ensured using relevant points from the CASP and COREQ checklists, considering the study's theoretical underpinning, design and aims. Reporting will be guided by the CONSORT and SRQR guidelines. [ABSTRACT FROM AUTHOR]

Introduction: Bacterial meningitis is a major cause of death, with an approximate case fatality rate of 37% across all age groups in South Africa. This study aimed to describe the demographic and pathogen characteristics of incident meningitis in children aged <1 year in South Africa from 2014 through 2018, during a period when Haemophilus influenzae type b vaccine and pneumococcal conjugate vaccines (PCV) were both included in the expanded program on immunization (EPI). Methods: We conducted a cohort study of routine laboratory data in the National Health Laboratory Service Corporate Data Warehouse, which covers approximately 80% of the South African population. We defined a case of laboratory-confirmed bacterial meningitis as any person aged <1 year with meningitis diagnosed by culture and identification of a pathogen documented as being a common cause of meningitis in CSF. The cause-specific incidence risks were calculated by dividing the number of positive specimens in each age group and year by the corresponding mid-year population for children under 1 year old and those in the post-neonatal period (≥ 28 days to 365 days old). For children under 28 days old, the annual numbers of registered livebirths were used. We used Poisson regression to compare the incidence of meningitis by year. Results: We identified 3575 (1.5%) cases of culture-confirmed bacterial meningitis from the 232,016 cerebrospinal fluid (CSF) specimens tested from 2014–2018. The highest number of cases were recorded in children aged <28 days (1873, 52.4%), male children (1800, 50.4%) as well as in the Gauteng Province (2014, 56.3%). Acinetobacter baumannii (14.9%), followed by Klebsiella pneumoniae (13.5%), and group B streptococcus (GBS) (10.7%), were the most common pathogens detected. Overall, A. baumannii had the highest incidence risk, occurring at 9.8 per 100,000 persons in children aged <1 year in 2018. Among neonates, A. baumannii peaked at 14.9 per 100,000 livebirths in 2018, while Streptococcus pneumoniae was most common in the post-neonatal period (≥ 28 days to 365 days old), peaking at 9.8 per 100,000 persons in 2014. There was an increase in the annual incidence of most pathogens over the study period. Conclusion: There was an increasing trend in the annual incidence of bacterial meningitis in infants caused by most pathogens, particularly A. baumannii, K. pneumoniae and GBS. In addition to increased uptake of vaccination, prevention measures to reduce nosocomial and mother-to-child transmission of bacteria could include antenatal screening for GBS in pregnant women, rigorous hygiene in the hospital environment as well as rational antibiotic use. [ABSTRACT FROM AUTHOR]

External HIV-related stigma remains pervasive, and its effect debilitating among PLHIV in South Africa, even though the country has made many advances against HIV. External HIV-related stigma impedes both HIV prevention and access to health care and reduces the quality of treatment and care received. This study examined the prevalence of and factors associated with higher levels of HIV-related stigma among youth and adults 15 years and older in South Africa. The analysis used a nationally representative population-based household survey data collected using a multistage cluster random sampling design. Exploratory factor analysis was used to calculate the primary outcome (higher and lower HIV stigma index scores above and below the mean, respectively), based on the total number of factors retained from the 10 item self-reported questions relating to attitudes and beliefs against PLHIV. Bivariate and multivariate generalised linear models with a log link and binomial distribution were fitted to estimate crude and adjusted risk ratios (ARR) with 95% confidence intervals (CI) for factors associated with external HIV-related stigma. Of 38 919 respondents, 49% (49.8%; 95% CI: 48.6–51.1) were categorised as having higher levels of external HIV-related stigma. Higher levels of HIV-related stigma were significantly associated with those who had secondary level education than those with no education/primary education [ARR = 1.14 (95% CI: 1.05–1.24), p = 0.002], those employed than unemployed [ARR = 1.08 (95% CI: 1.02–1.14), p = 0.006], those in rural areas than urban areas [ARR = 1.15 (95% CI: 1.07–1.23), p<0.001], those who were aware of their HIV status than not aware [ARR = 1.34 (95% CI: 1.12–1.61), p<0.001], those who were HIV positive than HIV negative [ARR = 1.09 (95% CI: 1.02–1.17), p = 0.018], and those with no correct HIV knowledge and myth rejection than their counterparts [ARR = 1.09 (95% CI: 1.03–1.15), p = 0.002]. The findings highlight the need for peer-facilitated HIV-stigma reduction interventions targeting all types of educational institutions and the strengthening of work-based interventions. The findings emphasise the prioritisation of rural informal settings/tribal areas when developing and implementing HIV stigma reduction interventions. The study suggests that stigma reduction should be considered an important component of HIV testing and awareness. Addressing public misconceptions about HIV can mitigate externalised stigma. [ABSTRACT FROM AUTHOR]

Cefazolin is an antibiotic used to prevent surgical site infections. During cardiac surgery with cardiopulmonary bypass (CPB), its efficacy target could be underachieved. We aimed to develop a population pharmacokinetic model for cefazolin in children and optimize the prophylactic dosing regimen. Children under 25 kg undergoing cardiac surgery with CPB and receiving cefazolin at standard doses (50 mg/kg IV every 4–6 h) were included in this analysis. A population pharmacokinetic model and Monte Carlo simulations were used to evaluate the probability of target attainment (PTA) for efficacy and toxicity with the standard regimen and an alternative regimen of continuous infusion, where loading and maintenance doses were calculated from model‐derived individual parameters. Twenty‐two patients were included, with median (range) age, body weight, and eGFR of 19.5 (1–94) months, 8.7 (2–21) kg, and 116 (48–159) mL/min, respectively. Six patients received an additional dose in the CPB circuit. A two‐compartment disposition model with an additional compartment for the CPB was developed, including weight‐based allometric scaling and eGFR. For a 10 kg patient with eGFR of 120 mL/min/1.73 m2, clearance was estimated as 0.856 L/h. Simulations indicated that the standard dosing regimen fell short of achieving the efficacy target >40% of the time within a dosing duration and in patients with good renal function, PTA ranged from <20% to 70% for the smallest to the largest patients, respectively, at high MICs. In contrast, the alternative regimen consistently maintained target concentrations throughout the procedure for all patients while using a lower overall dose. [ABSTRACT FROM AUTHOR]

Background: The increase in the prevalence of obesity in South Africa is a problem, and weight management plays an important role in the treatment of the high prevalence. For this purpose, a quantitative study was carried out using a structured questionnaire to assess the predictors of attempts and failures to lose weight. Methods: One thousand and fifty adults were enrolled in the study, and 54% (n = 562) were overweight and obese (BMI ≥ 25 kg/m2). Descriptive statistics were analysed. Inferential statistical tests (chi-square tests, logistic regression, and Pearson's correlation coefficients) were used to determine the relationship between variables. Results: The results revealed that age, sex, marital status, educational level, employment status, income level, area of residence, ethnic groups, and self-perceived BMI (normal weight, overweight, and obese) were predictors of the attempt and failure to lose weight (p < 0.05). There was a positive correlation between self-perceived BMI and the attempt and failure to lose weight, r = 0.2015 (p < 0.0001). Conclusion: Weight management is important in the realm of public health and can be regarded as an investment in the population, as it leads people toward weight control strategies grounded in empirical evidence. In addition, the findings can inform public health policies and interventions to improve weight management strategies at the population level. [ABSTRACT FROM AUTHOR]

In their efforts to dissuade Africans from engaging fruitfully on matters of design, including design anthropology, colonialists dismissed the indigenes as only capable of designing witchcraft and sorcery for which they were sadly famed in colonial anthropology. Arguing that twenty-first century African universities need to include design anthropology in the curriculum, this paper contends that the future of anthropology, and of Africa, lies in design as is evident in discourses and practices on designer babies, designer humanoid sex robots, industrial robots, designer robotic spouses, synthetic biology, Artificial Intelligence, human enhancements, nanofabrication, biohacking, gene and genome editing, reverse engineering and rewiring humans, gene and genome deletion, social designs and so on. Drawing on autoethnography and extensive literature review, the paper argues that design anthropology is increasingly becoming relevant in a world that is rethinking modernist designs which are at the core of the Anthropocene. Put differently, design anthropology enables [African] graduates to engage with contemporary, empirical issues of design in a twenty-first-century world where the discipline can only survive by shifting focus from an obsession with sterile discourses about, inter alia, the past and present of African witchcraft, culture, society and sorcery. [ABSTRACT FROM AUTHOR]

Global and South African populations continue to increase exponentially, with large flows of persons into urban centres. Urban air quality and bioaerosol are therefore becoming an increasingly important consideration in the face of a growing burden of allergic respiratory diseases. More than 20% of South Africans have asthma, with morbidity being disproportionately high compared to high-income countries (HICs). Air pollutants (particularly particulate matter and ozone), pollen and fungal spores are known triggers and exacerbating factors in asthma and, alarmingly, the levels of air pollutants remain high in hotspot areas despite robust air-quality legislation in South Africa. The concern is that genetically and environmentally vulnerable children in low- and middle-income countries (LMICs) such as South Africa will have asthma triggered and exacerbated disproportionately by poor air quality. Urban green areas are increasingly being recognised by many stakeholders as important possible mitigation tools. However, multiple factors must be considered for having an optimal green space design. In this review, we bring together experts across the fields of health, air quality and aerobiology to outline the intersection between asthma and air quality and/or aerobiology in South Africa. We highlight the importance of careful multidisciplinary green space design as a possible healing tonic. We conclude that detailed studies combining air quality, pollen dispersal and health issues are needed to derive more precise exposure--response functions of the green spaces in South Africa and other LMICs. [ABSTRACT FROM AUTHOR]

Background: South Africa has the highest adolescent asthma prevalence in Africa. Previous studies have found that the prevalence, risk factors and outcomes of asthma in adolescents vary with location and socio-economic factors. Methods: A narrative review of the literature was carried out on the prevalence, risk factors and outcomes of asthmatic adolescents in South Africa, with a focus on research gaps and priorities. Results: A variety of epidemiological asthma research has been conducted in South Africa; however, generalisable and comparable data have been generated by studies using the Global Asthma Network (GAN) survey and these show a high prevalence of asthma in adolescents (ranging from 13.7% in Durban to 20.3% in Cape Town). As in other sub-Saharan countries, a large proportion of adolescents have severe asthma without a diagnosis and no access to appropriate medication in the form of inhaled corticosteroids. Risk factors for asthma in South African adolescents are modifiable and related mainly to lifestyle and environmental exposures. Research on indoor and outdoor air pollution shows that these risk factors play a significant role in asthma outcomes, although there are only a few of these studies. Barriers to asthma control are patient-, healthcare- and disease-related, and qualitative research highlighting the perceptions and attitudes of those affected by asthma may assist in the co-development of tailored interventions to improve outcomes. Conclusion: Reducing the burden of asthma in South Africa will require a concerted effort towards prevention through a reduction in exposure, education about asthma, improved access to diagnosis and quality-assured medicines, and improved capacity in asthma diagnostics and clinical trials in children. [ABSTRACT FROM AUTHOR]

• Euphorbia bupleurifolia is exploited extensively for traditional medicine. • Harvesting is unsustainable as all plants are wild-sourced. • Whole plant is used for muthi resulting in destructive harvesting practices. • Participants harvest for personal use, treating patients and to sell. • Plant part substitution, use of fast-growing species and cultivation is recommended. Euphorbia bupleurifolia is a slow-growing dwarf succulent used for its medicinal properties by the Zulu and Xhosa people of South Africa. The latex reportedly cures several skin ailments, and it has been observed in traditional medicine (muthi) markets in KwaZulu-Natal and the Eastern Cape. To assess the impact of traditional medicine use and trade on E. bupleurifolia and gain insight into its demand and availability, surveys were conducted with traditional health practitioners (THPs) and traders in the KwaZulu-Natal, Eastern Cape, and Gauteng provinces using semi-structured questionnaires. These took place between November 2018 and January 2019. According to the participants (n = 23), an estimated 1721 E. bupleurifolia plants were harvested in a year for medicinal use. Approximately 6 % of participants harvested strictly for trade at muthi markets. Only 25 % of participants claimed to have noted a decline in wild populations, while 44 % believe the species is still abundant in the wild. However, only four E. bupleurifolia plants were encountered at a street market in King Williams Town (Eastern Cape province) while conducting surveys, suggesting that it has indeed become harder to find and hence scarcer in the wild. Common names of 10 other plant species which can be used as alternatives for E. bupleurifolia in healing were supplied by THPs, with uchithibhunga (Rhoicissus spp.) the most frequently cited. The high demand and noted declines in E. bupleurifolia in field surveys signifies the need for increased efforts directed at educating stakeholders on sustainable harvesting practices and promoting artificial propagation. [ABSTRACT FROM AUTHOR]

To date South African forensic anthropologists are only able to successfully apply a metric approach to estimate population affinity when constructing a biological profile from skeletal remains. While a non-metric, or macromorphoscopic approach exists, limited research has been conducted to explore its use in a South African population. This study aimed to explore 17 cranial macromorphoscopic traits to develop improved methodology for the estimation of population affinity among black, white and coloured South Africans and for the method to be compliant with standards of best practice. The trait frequency distributions revealed substantial group variation and overlap, and not a single trait can be considered characteristic of any one population group. Kruskal-Wallis and Dunn's tests demonstrated significant population differences for 13 of the 17 traits. Random forest modelling was used to develop classification models to assess the reliability and accuracy of the traits in identifying population affinity. Overall, the model including all traits obtained a classification accuracy of 79% when assessing population affinity, which is comparable to current craniometric methods. The variable importance indicates that all the traits contributed some information to the model, with the inferior nasal margin, nasal bone contour, and nasal aperture shape ranked the most useful for classification. Thus, this study validates the use of macromorphoscopic traits in a South African sample, and the population-specific data from this study can potentially be incorporated into forensic casework and skeletal analyses in South Africa to improve population affinity estimates. [ABSTRACT FROM AUTHOR]

In South Africa, many children with extensive support needs—including children who require AAC—are accommodated in care centers rather than the public schooling system. Caregivers employed at these centers need training in order to support children's communication using augmentative and alternative methods. A total of 29 center-based caregivers took part in this study. A single group pretest-posttest design was used to evaluate the effect of a 5-day training workshop on caregivers' ability to demonstrate and implement five AAC strategies. The effect of the workshop on caregivers' perceptions of their own skills as well as the social validity of the training were also evaluated. Results suggest that the workshop effectively taught caregivers to demonstrate the skills in a simulated roleplay situation. A proportion of caregivers also supplied video footage after the workshop that suggests the ability to implement the strategies in the care center contexts. Caregivers also perceived their own skills to have improved post workshop, and evaluated the training positively. [ABSTRACT FROM AUTHOR]

We demonstrate the value of a rapprochement between psychoanalytic work aimed at supporting marginalized mothers and discursive accounts of low-income mothers, providing a psychosocial analysis of data from an interview study with six low-income South African mothers. Employing discourse analysis, we show how instrumental mothering is a dominant and precarious construction in these mothers' talk. We use the concept of mentalization to track the affective work that accompanies the interactional emergence of this instrumental mothering discourse in a particular interview encounter. The implications of the research are discussed in the light of increasing demands for sociocultural responsive research and clinical practice. [ABSTRACT FROM AUTHOR]

Identification of Meloidogyne species is difficult using classical techniques alone, as some species have similar characteristics. Meloidogyne incognita is a plant-parasitic nematode that is one of the four most common species worldwide and has numerous hosts, including tomato (Solanum lycopersicum L.). This nematode is regarded as the most significant contributing factor to the reduction in tomato yield. Therefore, the present investigation was conducted in 2024 at the University of Limpopo to identify M. incognita. The COI mtDNA was sequenced and amplified from female nematodes, following which the blast result showed that the South African population exhibited a high similarity (100% identity) to those populations studied in China (MH743221 and MH743220). Utilizing the Maximum Likelihood method for phylogenetic analysis, it was demonstrated that our population and the other populations of M. incognita form a robust clade (87% bootstrap values). [ABSTRACT FROM AUTHOR]

The decline in the yield of tomato (Solanum lycopersicum L.) is largely attributed to the prevalence of nematodes belonging to the Meloidogyne species, commonly known as root-knot nematodes. The Meloidogyne species of nematodes are regarded as the most significant contributing factor to the reduction in tomato yield. Therefore, the present investigation was conducted in 2023 at the University of Limpopo to reveal that Meloidogyne javanica (Treub) Chitwood was the identified cause of the nematode. The ITS rDNA was sequenced and amplified from female nematodes, following which the blast result showed that the South African population exhibited a high similarity (99% identity) to those populations studied in the United States (AF387094 and U96305) and China (AY438555). A comparison to the USA population (AF387094), revealed four nucleotide differences. Utilizing the maximum likelihood method for phylogenetic analysis, it was demonstrated that our population and the other populations of M. javanica form a robust clade (91% bootstrap values). [ABSTRACT FROM AUTHOR]

Oral PrEP is highly effective against the acquisition of HIV but is underutilised by young women. New options, like the monthly dapivirine vaginal ring (DVR) and injectable long-acting cabotegravir (CAB-LA), are emerging. However, little is known about young women's perceptions of these alternatives. This qualitative study explored perceptions of the attributes of PrEP technologies in South Africa. Young women accessing sexual health services were purposively selected to participate in 22 in-depth interviews, five focus group discussions and two workshops using the nominal group technique, between August 2022 and March 2023. A thematic approach guided by the diffusion of innovation attributes, including relative advantage, compatibility with the student's lives, complexity of the technology, and trialability, was used for data analysis. The DVR was the least preferred because of lower efficacy, the perceived complexity of inserting it in the vagina and some safety concerns. Oral PrEP, which some had tried and discontinued, was least compatible with students' busy schedules. Integrating PrEP and contraceptives with similar return visit patterns could enhance service delivery. Intensive demand creation campaigns will be needed to increase PrEP utilisation and dispel myths about the vaginal ring. [ABSTRACT FROM AUTHOR]

Objective: Research documents effects of skin features on assessments of age, health and attractiveness of female faces. Ethnic variation also has been reported for the impact of age‐related changes in skin features on face assessments. Here, we investigate women's self‐ratings across age cohorts and ethnic groups and discrepancies with (non‐expert) assessor ratings of facial appearance together with age‐dependent changes in skin features. Methods: Faces of women 20–65 years from five ethnic groups (each n = 36) were imaged. Participants provided self‐ratings of age, health and attractiveness, and were judged on these attributes by members of the same ethnic group (each n = 120). Digital image analysis was used to quantify skin gloss, tone evenness, wrinkling and sagging. Age‐dependent changes in ratings and skin features within and between ethnic groups were assessed by comparing information from 10‐year cohorts. We also tested whether menopausal status could be predicted by self‐ratings, assessor ratings and image‐based skin features. Results: Women of all ethnic groups judged themselves younger and higher in attractiveness and health compared to third‐party assessors, with the largest discrepancies for age in French women and for attractiveness and health in South African women. In Indian and South African women, specular gloss and skin tone evenness were lower compared to other participants, and sagging was higher in Indian, Japanese and South African women compared to Chinese and French women. Women's menopausal status could be predicted from assessor ratings and image‐based skin features but not from self‐ratings. Conclusion: There are differences between women's self‐ratings and assessor ratings of facial appearance. These discrepancies vary with female age and ethnicity. Age and ethnicity effects also are evident in age‐dependent changes in skin features within and across ethnic groups, which together with assessor (but not self‐) ratings of facial appearance predict menopausal status. [ABSTRACT FROM AUTHOR]

On the occasion of 30 years of South African democracy, we reflect on the current state of Psychology research in South Africa. We conducted a situational analysis of all papers appearing in the South African Journal of Psychology (SAJP) and abstracts in PsycINFO with the keyword 'South Africa' over the last 5 years and compared the results with a previous review that used the same methodology. Findings show an increase in papers using 'hard' science approaches and a decrease in systems-oriented theories. Assessment remains a major topic. While COVID-19 and climate change featured, there remains a lack of or low focus on several key psycho-social issues experienced by South Africans. People living in poorer provinces and young and older people are under-represented in knowledge production. Collaborations or comparisons with other African or South American countries have decreased. Positively, production is being spearheaded by South African scholars or people affiliated with South African institutions. Using a decolonising lens that foregrounds epistemic justice, we conclude that substantial work remains to be done for knowledge production in South African Psychology to fulfil the decolonising imperative of distributive epistemic justice. [ABSTRACT FROM AUTHOR]

Visual and written accounts of rage in popular media tend to associate rage with violent masculinities.1 Filicide brings women’s rage into sharp relief. By reading South African women in relation to their ancestral peer, Sila van den Kaap and through a ‘black deformative’ analysis of media accounts and an interview of filicide in South Africa, this paper asserts that black women have ample cause for rage. Filicide brings the purportedly personal into public light and compels a response. Although the response tends to pathologise filicidal women, it calls attention to the conditions of impoverishment that black women live under. The paper suggests that filicidal women’s refusal of the conditions of mothering, may also be an enactment of a strike against gendered scripts of care. Their own subsequent suicide might simultaneously be read as an opting out and as reaching for freedom beyond the interdiction of impoverishment. The findings suggest that rage is a crucial action-oriented affect in the expression and striving for black freedoms of which women are always core protagonists. [ABSTRACT FROM AUTHOR]

Resilience, the ability to adapt well to adversity, is a critical factor in the well-being and success of young individuals. This study explored the multifaceted nature of resilience among South African youth challenged by COVID-related stressors and structural disadvantage. Specifically, this paper reports a follow-up qualitative leg of a sequential mixed-method design. Thirty-eight young adults (average age 24), all of whom reported high risk exposure but low depression symptoms, took part in semi-structured interviews to learn more about their resilience journey. Qualitative content analysis was employed to identify prominent themes. The study's findings emphasize the dynamic nature of youth resilience, with individuals being buoyed by social network support (especially family); personal agency, structural support, and resource combinations. The qualitative findings contribute to the growing attention to the need for comprehensive policies and programs that recognize and enhance multisystemic sources of support, enabling young individuals to navigate challenges and thrive in their transition into adulthood. [ABSTRACT FROM AUTHOR]

Introduction: Inflammation-induced remodelling of gestational tissues that underpins spontaneous preterm birth (sPTB, delivery < 37 weeks' gestation) may vary by race and context. To explore relationships between markers of these pathological processes, we (a) characterised the cervicovaginal fluid (CVF) cytokine profiles of pregnant South African women at risk of PTB; (b) determined CVF matrix-metalloproteinase-9 (MMP-9) and its regulator tissue inhibitor of metalloproteinase-1 (TIMP-1); and (c) explored the predictive potential of these markers for sPTB. Method of study: The concentrations of 10 inflammatory cytokines and MMP-9 and TIMP-1 were determined by ELISA in CVF samples from 47 non-labouring women at high risk of PTB. We studied CVF sampled at three gestational time points (GTPs): GTP1 (20-22 weeks, n = 37), GTP2 (26-28 weeks, n = 40), and GTP3 (34-36 weeks, n = 29) and analysed for changes in protein concentrations and predictive capacities (area under the ROC curve (AUC) and 95% confidence interval (CI)) for sPTB. Results: There were 11 (GTP1), 13 (GTP2), and 6 (GTP3) women who delivered preterm within 85.3 ± 25.9, 51.3 ± 15.3, and 11.8 ± 7.5 (mean ± SD) days after assessment, respectively. At GTP1, IL-8 was higher (4-fold, p = 0.02), whereas GM-CSF was lower (~1.4-fold, p = 0.03) in the preterm compared with term women with an average AUC = 0.73. At GTP2, IL-1β (18-fold, p < 0.0001), IL-8 (4-fold, p = 0.03), MMP-9 (17-fold, p = 0.0007), MMP-9/TIMP-1 ratio (9-fold, p = 0.004), and MMP-9/GM-CSF ratio (87-fold, p = 0.005) were higher in preterm compared with term women with an average AUC = 0.80. By contrast, IL-10 was associated with term delivery with an AUC (95% CI) = 0.75 (0.55-0.90). At GTP3, IL-1β (58-fold, p = 0.0003), IL-8 (12-fold, p = 0.002), MMP-9 (296-fold, p = 0.03), and TIMP-1 (35-fold, p = 0.01) were higher in preterm compared with term women with an average AUC = 0.85. Elevated IL-1β was associated with delivery within 14 days of assessment with AUC = 0.85 (0.67-0.96). Overall, elevated MMP-9 at GTP3 had the highest (13.3) positive likelihood ratio for distinguishing women at risk of sPTB. Lastly, a positive correlation between MMP-9 and TIMP-1 at all GTPs (r = 0.61, p < 0.01) for women delivering at term was only observed at GTP1 for those who delivered preterm (r = 0.70, p < 0.03). Conclusions: In this cohort, sPTB is associated with gestation-dependent increase in pro-inflammatory cytokines, decreased IL-10 and GM-CSF, and dysregulated MMP-9-TIMP-1 interaction. Levels of cytokine (especially IL-1β) and ECM remodelling proteins rise significantly in the final 2 weeks before the onset of labour when sPTB is imminent. The signalling mechanisms for these ECM remodelling observations remain to be elucidated. [ABSTRACT FROM AUTHOR]

AbstractPurposeMaterials and methodsResultsConclusionsThis study aimed to investigate the relationship between cardiometabolic disease risk and time spent in device-measured activity behaviours in a cohort of people with advanced osteoarthritis (OA) awaiting joint replacement surgery.Cardiometabolic risk biomarkers were assessed in people with OA (n = 96; hip n = 38, knee n = 58; mean (SD) age = 64.3 (9.8) years; 71% female). Physical activity (PA) and sedentary behaviour (SB) were measured by accelerometer over seven days (24 h/day).There were similar patterns of PA and SB between the hip and knee OA participants except for total number of steps (hip = 3365 (2926) vs knee 4344 (2836) steps/day; p = 0.018) and total stepping time (hip = 50.8 (38.2) vs knee = 67.2 (38.5) min/day; p = 0.005). Each additional cardiometabolic risk factor accumulated was associated with a 26.3 min/day increase in sedentary behaviour (p = 0.032; 95% CI: 2.3, 50.2), a 26.3 min/day decrease in upright time (p = 0.032; −50.2, –2.3) and a 23.6 min/day decrease in standing time (p = 0.032; −45.1, –2.1).In people with hip or knee OA, increased cardiometabolic disease risk was associated with more sitting and less upright and standing time. Findings support targeting reductions in sedentary behaviour for improvements in cardiometabolic health in people with osteoarthritis.IMPLICATIONS FOR REHABILITATIONKnee and hip osteoarthritis is a condition which is associated with an increased risk of cardiometabolic disease but also due to the low levels of physical activity and high levels of sedentary behaviour.Offsetting sedentary behaviour with light physical activity offers a feasible interventional target to reduce the risk of cardiometabolic disease in people with hip and knee osteoarthritis.Knee and hip osteoarthritis is a condition which is associated with an increased risk of cardiometabolic disease but also due to the low levels of physical activity and high levels of sedentary behaviour.Offsetting sedentary behaviour with light physical activity offers a feasible interventional target to reduce the risk of cardiometabolic disease in people with hip and knee osteoarthritis. [ABSTRACT FROM AUTHOR]

Critically endangered Hooded Vultures (Necrosyrtes monachus Temminck, 1823), like many vulture species globally, are experiencing rapid population declines due to anthropogenic factors such as poisonings, human persecution, trading for belief-based use, and habitat loss/degradation. The Hooded Vulture is widespread across sub-Saharan Africa. Although it is considered one of the most abundant vultures in West Africa, this vulture species is less common in East and southern Africa, with the population at the southern-most edge of the distribution (in South Africa and Eswatini) estimated at only 100–200 mature individuals. The distribution of Hooded Vultures has contracted dramatically in southern Africa, with breeding populations largely confined to protected areas such as the Greater Kruger National Park. This study aimed to investigate the genetic diversity of the southern African range-edge population and assess if the recent contraction in the distribution has resulted in the population experiencing a genetic bottleneck. Sixteen microsatellite loci were amplified for samples collected along the Olifants River in the Greater Kruger National Park (n = 30). The genetic diversity in the South African population was compared to samples (n = 30) collected in Ghana, where Hooded Vultures are more abundant. Contrary to expectations, the South African peripheral Hooded Vulture population showed higher levels of heterozygosity (HO = 0.495) than the Ghanaian population (HO = 0.315). Neither population showed signs of recent bottleneck events when tested using demographic modelling and Approximate Bayesian computation (ABC). However, both populations showed high levels of inbreeding and relatedness. Our results suggest that despite being a small peripheral population, the South African Hooded Vulture population showed a similar level of genetic diversity as individuals sampled from a core population within the species distribution (in Ghana). This study supports the need for Hooded Vulture conservation efforts in the southern African region and highlights the evolutionary importance of range-edge populations. [ABSTRACT FROM AUTHOR]

With the study reported on here we aimed to determine and compare teacher perceptions of the concept of virtual reality between Turkish and South African teachers using metaphors. We adopted a phenomenological approach, and a questionnaire was administered to a total of 100 teachers in both countries, using random sampling. The study included 4 schools - 2 secondary schools selected from each country. The data obtained were analysed and categorised into themes using the metaphor mismatch technique. The following themes were identified and interpreted, learning, entertainment, technology, art, travel, and imagination. Teachers presented metaphors based on relevance to their area of practice and perceived potential use of virtual reality in teaching and learning. Based on the number of metaphors identified, teachers held a narrow perception in the categories, entertainment, technology, and travel, while more metaphors, with varied sample explanation responses were identified in the categories of learning, art, and imagination. The findings of this study can be used to develop a user road map for teacher professional development and to inform policy on the use of virtual reality in teaching and learning. [ABSTRACT FROM AUTHOR]

The inclusion of culturally and linguistically diverse learners in schools remains a global challenge and South African schools are no exception. The increasing number of national and international immigrants and refugees contributes to cultural and linguistic diversity (CLD) in classrooms. Teachers thus grapple with the inclusion of CLD learners. In this study we explored the perspectives of teachers on strategies that could enhance the inclusion of CLD learners in secondary schools. We contend that the inclusion of CLD learners has not been fully addressed in South African schools. A qualitative phenomenological design located within the interpretivism paradigm was used. We employed culturally responsive pedagogy as theoretical framework for the study. From a population of South African teachers in Gauteng, South Africa, 12 secondary school teachers were sampled through critical case sampling. Data were generated through interviews. Research findings identify the need for amendment of government policies, in-service training, and workshops for teachers, incorporating ethnically and culturally diverse content during instruction. Within this study, we extend awareness of the strategies that teachers in CLD classrooms can use to enhance inclusive education and propose further strategies that can also be considered in teaching in CLD classrooms. It is concluded that there is a great need to incorporate inclusive education in the higher education curriculum to capacitate pre-service teachers on the inclusion of learners - CLD learners included. [ABSTRACT FROM AUTHOR]