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Carbohydrate intolerance, commonly linked to the consumption of lactose, fructose, or sorbitol, affects up to 30% of the population in high-income countries. Although sorbitol intolerance is attributed to malabsorption, the underlying mechanism remains unresolved. Here, we show that a history of antibiotic exposure combined with high fat intake triggered long-lasting sorbitol intolerance in mice by reducing Clostridia abundance, which impaired microbial sorbitol catabolism. The restoration of sorbitol catabolism by inoculation with probiotic Escherichia coli protected mice against sorbitol intolerance but did not restore Clostridia abundance. Inoculation with the butyrate producer Anaerostipes caccae restored a normal Clostridia abundance, which protected mice against sorbitol-induced diarrhea even when the probiotic was cleared. Butyrate restored Clostridia abundance by stimulating epithelial peroxisome proliferator-activated receptor-gamma (PPAR-γ) signaling to restore epithelial hypoxia in the colon. Collectively, these mechanistic insights identify microbial sorbitol catabolism as a potential target for approaches for the diagnosis, treatment, and prevention of sorbitol intolerance.

Biallelic SORD mutations cause one of the most frequent forms of recessive hereditary neuropathy, estimated to affect ∼10 000 patients in North America and Europe alone. Pathogenic SORD loss-of-function changes in the encoded enzyme sorbitol dehydrogenase result in abnormally high sorbitol levels in cells and serum. How sorbitol accumulation leads to peripheral neuropathy remains to be elucidated. A reproducible animal model for SORD neuropathy is essential to illuminate the pathogenesis of SORD deficiency and for preclinical studies of potential therapies. Therefore, we have generated a Sord knockout (KO), Sord −/−, Sprague Dawley rat, to model the human disease and to investigate the pathophysiology underlying SORD deficiency. We have characterized the phenotype in these rats with a battery of behavioural tests as well as biochemical, physiological and comprehensive histological examinations. Sord −/− rats had remarkably increased levels of sorbitol in serum, CSF and peripheral nerve. Moreover, serum from Sord −/− rats contained significantly increased levels of neurofilament light chain, an established biomarker for axonal degeneration. Motor performance significantly declined in Sord −/− animals starting at ∼7 months of age. Gait analysis evaluated with video motion-tracking confirmed abnormal gait patterns in the hindlimbs. Motor nerve conduction velocities of the tibial nerves were slowed. Light and electron microscopy of the peripheral nervous system revealed degenerating myelinated axons, de- and remyelinated axons, and a likely pathognomonic finding—enlarged 'ballooned' myelin sheaths. These findings mainly affected myelinated motor axons; myelinated sensory axons were largely spared. In summary, Sord −/− rats develop a motor-predominant neuropathy that closely resembles the human phenotype. Our studies revealed novel significant aspects of SORD deficiency, and this model will lead to an improved understanding of the pathophysiology and the therapeutic options for SORD neuropathy. [ABSTRACT FROM AUTHOR]

Gluconic acid and sorbitol are among the value-added chemicals that can be derived from biomass. Nowadays, these compounds are typically produced through biotechnological processes, but electrochemical methods offer numerous advantages over alternative approaches. While studies have extensively explored metals like copper, palladium, gold, and platinum, nickel has received relatively limited attention in this context. Notably, nickel exhibits electrochemical activity suitable for organic electrosynthesis. This work has been achieved with 5-h long-term electrolysis, glucose as a reactant, utilizing modified nickel electrodes in a KOH solution. While these studies achieved substantial conversion rates, the selectivities and Faraday efficiencies toward gluconic acid and sorbitol remained comparatively low. The long-term electrolysis of glucose using modified nickel electrodes resulted in the identification of various side products. These include formic acid, oxalic acid, glycolic acid, tartronic acid, glyceric acid, and arabinose. [ABSTRACT FROM AUTHOR]

Consolidated bioprocessing candidate, Clostridium thermocellum, is a cellulose hydrolysis specialist, with the ability to ferment the released sugars to produce bioethanol. C. thermocellum is generally studied with model substrates Avicel and cellobiose to understand the metabolic pathway leading to ethanol. In the present study, adaptive laboratory evolution, allowing C. thermocellum DSM 1237 to adapt to growth on glucose, fructose, and sorbitol, with the prospect that some strains will adapt their metabolism to yield more ethanol. Adaptive growth on glucose and sorbitol resulted in an approximately 1 mM and 2 mM increase in ethanol yield per millimolar glucose equivalent, respectively, accompanied by a shift in the production of the other expected fermentation end products. The increase in ethanol yield observed for sorbitol adapted cells was due to the carbon source being more reduced compared to cellobiose. Glucose and cellobiose have similar oxidation states thus the increase in ethanol yield is due to the rerouting of electrons from other reduced metabolic products excluding H2 which did not decrease in yield. There was no increase in ethanol yield observed for fructose adapted cells, but there was an unanticipated elimination of formate production, also observed in sorbitol adapted cells suggesting that fructose has regulatory implications on formate production either at the transcription or protein level. [ABSTRACT FROM AUTHOR]

Jackfruit seeds have been explored as one interesting alternative starch source to be used in the production of sustainable bioplastics. In this context, this work aimed to investigate the effect of sorbitol concentration, one alternative plasticizer to glycerol, and the starch properties of the films. A complete factorial experimental study was done based on five different concentrations of starch (2, 3, 4, 5, and 6% w/w) and sorbitol (40, 50, 60, 70, 80 g per 100 g of starch). The films were produced by casting technique and were characterized about thickness, optical properties, moisture resistance, mechanical properties, and morphology. The optical properties revealed opaque films, with opacity values ranging from 16.04 to 21.26%. Regarding moisture resistance, the less hydrophilic films showed lower plasticizer content. The water solubility varied from 25.48 to 38.93%; the water vapor permeability ranged from 9.99 to 11.82 × 10−9 (g/m s Pa) and the free energy of hydrophobic interactions varied from − 28.16 to 40.13 mJ m−1. Concerning the mechanical properties, the tensile strength of the films ranged from 3.55 to 9.25 MPa, and increasing the sorbitol concentration decreased the tensile strength and increased the elongation at break. The morphology and structure analysis showed that starch granules were not fully gelatinized, with microbubbles and grooves in the film matrix. This study showed the great potential of jackfruit seed starch and sorbitol in the manufacture of biodegradable films as alternative sustainable packaging. [ABSTRACT FROM AUTHOR]

Poly[(butylene adipate)‐co‐terephthalate] (PBAT) is currently the most widely used and versatile petroleum‐based fully biodegradable polyester, drawing extensive attention from researchers. However, the high production cost of PBAT restricts its widespread application. Currently, incorporating fillers into PBAT materials is considered the most effective approach to reduce production costs, with thermoplastic starch recognized as the optimal filler for PBAT base materials. Nevertheless, the low mechanical strength of thermoplastic starch significantly compromises the performance of PBAT base materials. In this study, thermoplastic starch with higher mechanical strength was prepared by partially substituting commonly used glycerol with a higher molecular weight sorbitol as the plasticizer. The enhanced thermoplastic starch was then used as a filler for PBAT materials, leading to the fabrication of PBAT‐based blend films with high starch content. Mechanical property tests revealed a 52.2% and 65.3% increase of tensile strength in the transverse and longitudinal directions, respectively, when sorbitol partially replaced glycerol as the plasticizer for thermoplastic starch. Scanning electron microscopy results demonstrated improved dispersion of thermoplastic starch particles in PBAT when sorbitol and glycerol were used together. Meanwhile, the thermal performance and stability of PBAT were not significantly affected by the thermoplastic starch filling. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

SUMMARY: In eggs, the albumen and yolk undergo physicochemical changes during storage, which will decrease egg quality. Several factors can contribute to the loss of egg quality (such as temperature, humidity, duration of storage, thin eggshell, age of the flock). Coatings are a tool that can help maintain egg quality during long storage periods. The standard composition of egg coatings includes polysaccharides, lipids, proteins and polyols that can be used as plasticising substances and incorporated to induce flexibility in films. Glycerol is the most commonly used plasticiser in egg coatings due to its abundance and relatively low cost. However, other substances have demonstrated better results when added to coatings to extend the shelf life of eggs. Few studies have investigated the relationship between polyol plasticisers and the different bases for preparing coatings. Furthermore, little discussion exists about which levels should be incorporated into the egg-coating solution. The real impact of using polyols in egg coatings still has some gaps. This review gives an overview of the current use of polyols and trends in developing new egg coatings. [ABSTRACT FROM AUTHOR]

BACKGROUND: The present study aimed to investigate the cryoprotective effect of epigallocatechin gallate (EGCG) replacing sucrose on surimi during frozen storage. Substitution or partial substitution of 0.1% EGCG for sucrose (1.5%) was added to surimi, and the surimi samples without and with commercial cryoprotectants (4% sucrose and 4% sorbitol) were used as the control group. RESULTS: The results obtained suggest that, with the increase in frozen storage time, the structural performance of surimi protein gradually weakened (e.g. the decrease in the surface hydrophobicity, the increase in the total sulfhydryl and solubility, and the protein myosin heavy chain bands became shallow) and surimi gel quality gradually deteriorated (e.g. the decrease in water‐holding capacity, gel strength and all texture profile attributes). However, compared with the other three group surimi samples during the frozen period, the surimi proteins with partial replacement of sucrose by EGCG had a higher total sulfhydryl group content and solubility of proteins, as well as lower surface hydrophobicity of protein, suggesting that the addition of EGCG as a partial substitute for sucrose can enhance the antifreeze ability of surimi. Meanwhile, the surimi gel with the partial replacement of sucrose by EGCG had a higher water retention capacity, gel strength and texture attributes (e.g. hardness, springiness, cohesiveness, chewiness, and resilience), indicating that the addition of EGCG as a partial substitute for sucrose can inhibit the deterioration of surimi gel quality. CONCLUSION: Overall, EGCG partially replacing sucrose can play an alternative cryoprotectant with a lower sweetness to prevent the quality of surimi from deteriorating. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The corrosion behavior of Q345qNH steel and Q420qNH steel in simulated industrial atmospheric environment medium was studied by periodic immersion+infrared aging corrosion experiment. The results show that the corrosion type of both samples is uneven comprehensive corrosion, and the rust layer formed in the later stage of corrosion is relatively dense. But average corrosion rate of Q345qNH steel is always lower than that of Q420qNH steel, and the ratio of Iα‐FeOOH/Iγ‐FeOOH in rust layer is always higher. Compared with Q420qNH steel, Q345qNH steel has fewer surface pits but deeper local pits. The self‐corrosion potential of Q345qNH steel increases obviously, the resistance of the rust layer is larger, and protection to the matrix is stronger. This is because the formation of a large number of corrosion microcells induced by fine lamellar sorbite tissue that uneven distributed in Q420qNH steel, which increases the corrosion rate and makes corrosion uneven, while the larger pearlitic group in Q345qNH steel increases the local corrosion rate. However, the higher chromium/carbon ratio in Q345qNH steel promotes the conversion of lepidocrocite to goethite and inhibits the cathode reaction in the infrared drying stage, improving the density and stability of the rust layer. [ABSTRACT FROM AUTHOR]

SUMMARY Pear is a widely cultivated fruit crop, but its distribution and sustainable production are significantly limited by salt stress. This study used RNA‐Seq time‐course analysis, WGCNA, and functional enrichment analysis to uncover the molecular mechanisms underlying salt stress tolerance in Pyrus ussuriensis. We identified an ABA‐related regulatory module, PbGBF3‐PbAPL2‐PbSDH1, as crucial in this response. PbGBF3, a bZIP transcription factor, enhances salt tolerance by upregulating PbAPL2 and PbSDH1. Overexpression of PbGBF3 improved salt tolerance in Pyrus communis calli and Arabidopsis, while silencing it reduced tolerance in Pyrus betulifolia. Functional assays showed that PbGBF3 binds to the promoters of PbAPL2 and PbSDH1, increasing their expression. PbAPL2 and PbSDH1, key enzymes in starch synthesis and the sorbitol pathway, respectively, enhance salt tolerance by increasing AGPase activity, soluble sugar content, and SDH activity, improving ROS scavenging and ion balance. Our findings suggest that the PbGBF3‐PbAPL2 and PbGBF3‐PbSDH1 modules positively regulate salt tolerance by enhancing ABA signaling and reducing ABA‐mediated growth inhibition. These insights provide a foundation for developing salt‐tolerant pear cultivars. [ABSTRACT FROM AUTHOR]

Ru/N-doped activated carbon/NbOPO4 material bearing unique surface chemistry (Brønsted acidic NbOPO4, Lewis basic N-groups and oxygen functional groups) is shown to exhibit exceptional catalytic activity upon hydrolytic hydrogenation of cellobiose into sorbitol under mild conditions. The multifunctional catalyst was synthesized mechanically grinding NbOPO4 with Ru/N-doped activated carbon under ambient conditions and characterized by standard techniques. The multifunctional catalyst outperformed the benchmark Ru catalysts viz. Ru/carbon, Ru/N-doped activated carbon, Ru/SBA-15, Ru/NbOPO4, Ru/Nb2O5, and Ru/NbOPO4-N-doped activated carbon (obtained by sequential deposition of NbOPO4 and Ru on N-doped carbon) with respect to desired product yield as well as carbon balance closure. Under optimized conditions (140 °C, 4 h, and 20 bar H2), sorbitol yields up to ~ 90% could be obtained with the Ru/N-doped carbon/NbOPO4 catalyst; analysis of reaction products in the temperature range of 130–150 °C revealed cellobitol as the main intermediate. Most importantly, the catalytic material demonstrated good operational stability and demonstrated excellent stability in a fixed-bed reactor under continuous flow conditions, maintaining constant activity, and sorbitol selectivity during ~ 110 h operation. The exceptional performance and stability of the multifunctional catalyst were attributed to the surface chemistry of carbon support that stabilized the Ru nanoparticles, and the acidic NbOPO4 phase promoting efficient and selective hydrolysis of cellobiose and cellobitol. [ABSTRACT FROM AUTHOR]

Sugar is vital for plant growth and determines fruit quality via its content and composition. This study explores the differential sugar accumulation in two plum varieties, 'Fengtangli (FTL)' and 'Siyueli (SYL)'. The result showed that 'FTL' fruit displayed higher soluble solids and sugar content at various development stages. Metabolomic analysis indicated increased sorbitol in 'FTL', linked to elevated sorbitol‐6‐phosphate‐dehydrogenase (S6PDH) activity. Transcriptome analysis identified a key gene for sorbitol synthesis, PsS6PDH4, which was significantly higher expressed in 'FTL' than in 'SYL'. The function of the PsS6PDH4 gene was verified in strawberry, apple, and plum fruits using transient overexpression and virus‐induced gene silencing techniques. The results showed that overexpression of the PsS6PDH4 gene in strawberry, apple, and plum fruits promoted the accumulation of soluble solids content and sorbitol, while inhibition of the gene reduced soluble solids content and sorbitol content. Meanwhile, analysis of the relationship between PsS6PDH4 gene expression, sorbitol, and soluble solids content in four different plum varieties revealed a significant correlation between PsS6PDH4 gene expression and soluble solids content as well as sorbitol content. This research discovered PsS6PDH4 as a crucial regulator of sugar metabolism in plum, with potential applications in improving fruit sweetness and nutritional value in various fruit species. Understanding these molecular pathways can lead to innovative approaches for enhancing fruit quality, benefiting sustainable agriculture and consumer preferences in the global fruit industry. [ABSTRACT FROM AUTHOR]

With the growing global demand for biodegradable plastics, the hydrogenation of dimethyl maleate (DMM) to produce 1,4-butanediol (BDO), raw materials for poly(butylene succinate), has become one of the hot spots in chemical industry. In this work, we synthesized a series of Cu@C/ZnO–Sx catalysts by the method of sorbitol modification for the liquid-phase hydrogenation of DMM. The modification with appropriate sorbitol led to a decoration of carbon on catalyst surface, which improved the copper dispersion and Cu+/(Cu0 + Cu+) molar ratio in the Cu@C/ZnO–S0.08 catalyst. The carbon decoration not only promoted the formation of more copper species having stronger interaction with ZnO, but also enhanced the absorption of ester on catalyst surface. These distinctive properties contributed to the high performance of the Cu@C/ZnO–S0.08 catalyst in DMM hydrogenation, achieving an excellent BDO yield of 98.4% and superior reusability. The insights into the roles of sorbitol modification could give valuable guidance to the further design of high performance catalysts for esters hydrogenation. [ABSTRACT FROM AUTHOR]

Due to the presence of the boronic acid moieties, poly-3-thienylboronic acid has an affinity for saccharides and other diol-containing compounds. Thin films of this novel chemosensitive polymer were synthesized electrochemically on the gold surface. The adhesion of the polymer was enhanced by the deposition of a monomolecular layer of thiophenol. The technology was used to fabricate conductometric sensors for glucose and other diol-containing compounds. Simultaneous two- and four-electrode conductivity measurements were performed. The chemical sensitivity to sorbitol, fructose, glucose, and ethylene glycol was studied at different pH and electrode potentials, and the corresponding binding constants were obtained. Depending on the electrode potential, the reciprocal values of the binding constants of glucose to poly-3-thienylboronic acid at neutral pH are in the range of 0.2 mM–1.0 mM. The affinity for glucose has been studied in buffer solutions and in solutions containing the major components of human blood. It was shown that the presence of human serum albumin increases the affinity of poly-3-thienylboronic acid for diol-containing compounds. [ABSTRACT FROM AUTHOR]

BACKGROUND: Sorbitol as a sweetener is often thought to be unable to participate in the Maillard reaction causing browning. However, browning of a system was found to be significant when sorbitol was mixed with glycine and heated. The thiol compounds glutathione and cysteine were added to the system, and the inhibition mechanism of the two on the browning of the system was studied by combining the changes of precursor substances, intermediate products and browning degree. RESULTS: When the concentration of thiol compounds reached 25 mg mL−1, both could make the browning inhibition of the system more than 80%, and the accumulated glucose concentration was reduced to <35% of the control. The production of 3‐deoxyglucosone, a precursor of melanoidin, was significantly reduced. CONCLUSION: Glutathione and cysteine directly inhibited the production of substrates in the sorbitol/glycine system, reduced glucose accumulation through competitive consumption and captured highly active intermediates through sulfhydryl groups. This has implications for the browning control of food systems containing sugar alcohols. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Aqueous‐phase reforming (APR) is an interesting technique for generating hydrogen (H2) from biofeeds. In this work, APR of model compounds of wet biomass for H2 production was investigated. Glycerol, sorbitol, and glycine were the chosen model compounds. They represent polyols and amino acids in wet biomass such as waste sludge and microalgal biomass. The Pt/Al2O3 catalyst was preferred and it was characterized using nitrogen adsorption–desorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM), x‐ray diffraction (XRD), and x‐ray photoelectron spectroscopy (XPS) techniques. APR trials were performed in a continuous fixed‐bed reactor. The reaction conditions chosen for this work were: temperature (T) 453–498 K, pressure (P) 1.2–2.4 MPa, feed concentration 5–15 wt%, and weight hourly space velocity (WHSV) 0.15–0.6 g reactant/(g catalyst h). The best conditions for H2 production by the APR process were found to be T = 498 K, P = 2.4 MPa, and feed concentration = 15 wt%. Among the chosen model compounds, glycerol exhibited the highest H2 selectivity (82.7%) and H2 yield (21.6%) at 498 K. The analysis of kinetic data suggested first‐order reaction kinetics for all the model compounds. The values of activation energy for the reactions with glycerol (55.4 kJ/mol), sorbitol (51.6 kJ/mol), and glycine (45.7 kJ/mol) were determined. Thus, APR is a promising route for effectively producing H2‐bearing gaseous products with high heating value from wet biomass. [ABSTRACT FROM AUTHOR]

Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, the challenge of regeneration in its natural environment makes it a vulnerable species. In this context, various treatments for the in vitro conservation of explants were evaluated in the present study. Conservation by slow growth, the effects of osmotic substances such as sorbitol, mannitol, and sucrose at different concentrations were assessed. Different concentrations of MS and B5 culture media were also examined for their impact on the growth, budding, mortality, and rooting of explants over 12 months without subcultures. For long-term conservation by cryopreservation, two techniques were tested: vitrification and encapsulation-dehydration. Short-term preservation of explants in sorbitol resulted in low mortality, minimal growth, and limited development of new shoots compared to preservation in sucrose or mannitol, although tissues could be recovered successfully from all storage conditions. After cryopreservation and 45 days of recovery, explants with the lowest mortality (4%) were from the control treatment (without cryoprotection) cultivated in a medium with sucrose which proved useful as a cryoprotectant. In conclusion, it is possible to conserve C. officinalis tissues in the short-term using in vitro techniques, while further assays are needed for long-term conservation. [ABSTRACT FROM AUTHOR]

This field experiment was conducted to investigate the effects of zeolite amendment and foliar applications of copper, both in normal and nano forms, combined with sorbitol spray on the growth, yield, and grain quality of wheat (Triticum aestivum L.) under varying mineral nitrogen doses. The scientific aim was to evaluate the potential of these treatments to improve wheat performance and nutrient use efficiency while reducing nitrogen fertilizer inputs (ammonium sulphate, 20.6 % N). The experiment followed a split-split plot design, with nitrogen fertilizer rates (100% and 80% of the recommended dose, equivalent to 75 and 60 kg N per feddan, respectively) as the main plots, zeolite application ( applied at rate of 1.7 ton fed-1 or not), as the sub-plots, and foliar applications [control (without), copper sulphate (CuSO4.7H2O, 25 % Cu, 20 mg Cu L-1), sorbitol (500 mM L-1), copper oxide Nanoparticles (CuO NPs, 2.0 ng L-1), sorbitol + mineral Cu as combined treatment, sorbitol+ nano Cu as combined treatment) as sub-sub plots. Results showed that higher nitrogen doses generally increased growth parameters, grain yield, and grain quality attributes, but zeolite incorporation significantly enhanced these parameters even at lower nitrogen levels. Additionally, foliar applications of copper, especially in nano form, combined with sorbitol, further improved wheat performance, emphasizing the importance of integrated nutrient management strategies. Notably, the presence of zeolite mitigated the negative effects of reduced nitrogen doses, highlighting its role in enhancing nutrient use efficiency and crop resilience. The most significant findings included increased grain yield, nitrogen content, protein content, and total sugar content in wheat grains with zeolite application and foliar treatments. The interaction effects demonstrated the synergistic benefits of zeolite with foliar applications, particularly in improving nitrogen and protein contents. Also, the obtained results confirmed that zeolite possessed a vital role in improving soil fertility via raising the soil content of NPK as well as water holding capacity (WHC). Overall, the study underscores the potential of zeolite and foliar applications as effective tools for optimizing wheat production while reducing environmental impacts associated with excessive nitrogen fertilization. It is recommended to further explore these treatments under diverse agro-climatic conditions and crop systems to validate their efficacy and scalability. Additionally, future research should focus on elucidating the mechanisms underlying the observed improvements in nutrient uptake, utilization efficiency, and crop performance to optimize their application strategies for sustainable agriculture. [ABSTRACT FROM AUTHOR]

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Alloy steel 40Cr was treated by plasma oxynitriding after quenching and tempering. The microstructures, mechanical properties, and tribological performances of plasma oxidized–nitrided layer at tempering temperatures of 500, 520, and 540 °C were investigated. The typical tempered sorbite structure was present when the 40Cr alloy steel was treated by quenching at 900 °C and tempering. A special structure of mixed oxide–nitride layer along with the veinlike nitride phase was formed after plasma oxynitriding. The surface hardness of the quenched–tempered 40Cr alloy steel was significantly improved. The quenched 40Cr alloy steel tempered at 520 °C exhibited the highest hardness of approximately 973 HV0.2 and the lowest wear rate of about 1.72 × 10−14 m3 N−1 m−1 than the samples tempered at 500 and 540 °C. The oxide and nitride phases inside the plasma oxidized–nitrided layer played important roles in the improvement of the surface mechanical properties and the tribological performance of the quenched–tempered alloy steel. [ABSTRACT FROM AUTHOR]

Strawberry (Fragaria × ananassa Duch.) is one of the most widely consumed and cultivated fruits worldwide. Sorbitol plays a role in plant responses to many biotic and abiotic stresses. In this research, we intended to understand the effect of sorbitol spraying on the bioactive compounds of strawberry leaves. The application of sorbitol at different concentrations (0, 25, 50 mM and 75 mM) greatly improved strawberry characteristics such as total chlorophyll, chlorophyll a and b, carotenoids, and total phenolics. As sorbitol concentrations increased, chlorophyll a and chlorophyll b values increased in the samples taken during the fruiting period and higher values were obtained. The carotenoid content increased by approximately 189.49% and the total phenolic content increased by 30.85% in strawberry plants treated with sorbitol compared with the control. Supply of sorbitol decreased flavonoid content. The results indicate that sorbitol treatment has no inhibitory influence on the overall growth of strawberries. Among the biochemical parameters analyzed, chlorophyll, phenolic, and carotenoid contents increased, whereas flavonoid content decreased with sorbitol application.

Isosorbide, a bicyclic C6 diol, has considerable value as a precursor for the production of bio-derived polymers. Current production of isosorbide from sorbitol utilizes homogeneous acid, commonly H2SO4, creating harmful waste and complicating separation. Thus, a heterogeneous acid catalyst capable of producing isosorbide from sorbitol in high yield under mild conditions would be desirable. Reported here is a quantitative investigation of the liquid-phase dehydration of neat sorbitol over sulfated zirconia (SZ) solid acid catalysts produced via sol-gel synthesis. The catalyst preparation allows for precise surface area control (morphology) and tunable catalytic activity. The S/Zr ratio (0.1-2.0) and calcination temperature (425-625 °C) were varied to evaluate their effects on morphology, acidity, and reaction kinetics and, as a result, to optimize the catalytic system for this transformation. With the optimal SZ catalyst, complete conversion of sorbitol occurred in

BACKGROUND: Social support and social networks have long been postulated to impact health outcomes but their impact on life expectancy and disability in older adults remains poorly quantified. METHODS: As part of the Cardiovascular Health Study, we followed 5,749 adults aged 65 years and older from 4 US field centers for 25 years. We assessed the Lubben social network score [range 0-50] and a social support score [range 0-24] derived from the Interpersonal Support Evaluation List (ISEL-12) in two consecutive years starting at study recruitment. We used remaining years of life (YOL) from study enrollment to death to approximate life expectancy. We defined years of active life (YAL) as the number of study years in which participants lived without any difficulties in activities of daily living. We used compression of disability to reflect the proportion of life lived able (YAL/YOL). We used linear regression to adjust for socio-demographics and comorbidity. RESULTS: The mean (standard deviation [SD]) scores were 32.3 ± 6.8 points for social network score and 8.3 ± 2.4 points for social support score. For every 1-SD increase in social network score, adjusted participant life expectancy was 0.40 years higher (95% CI 0.22-0.58; p

An experiment was conducted in Polytunnel house at Longitude 43.30, latitude 33.410 the Department of Horticulture, College of Agriculture, University of Anbar, during the spring season of 2023. The experiment was designed to investigate the effects of spraying with potassium, a combination of alcohol sugar (sorbitol), and boron on the vegetative traits and chemical content of melon leaves under protected cultivation conditions. The study included two factors: the first factor was spraying with potassium at three concentrations: 0, 4, and 8 gm L-1, and it was coded as K0, K1, and K2, respectively. The second factor was spraying with a combination of sorbitol and boron at five concentrations: the first treatment was the comparison (0 boron and 0 sorbitol), the second (20 milligrams liter-1 boron + 25 grams liter-1 sorbitol), the third (20 milligrams/liter boron + 50 grams liter-1 sorbitol), the fourth (40 milligrams liter-1 boron + 25 grams liter-1 sorbitol), and the fifth (40 milligrams liter-1 boron + 50 grams liter-1 sorbitol To obtain the best growth and yield from it ), and it was coded where to T0, T1, T3, , and T4, respectively. Potassium was sprayed when 50% of the flowers appeared and until the fruit ripened at a rate of one spray per week. As for the combination of boron and sorbitol, it was sprayed after the appearance of 6-8 true leaves at a rate of three sprays between sprays every two weeks. The experiment was conducted as a factorial experiment within a complete randomized block design with three replicates. The study showed that the second potassium concentration (K1) achieved the highest vegetative growth traits (plant height, leaf area, number of nodes, and dry weight of the total vegetation, which were 311.98 cm, 95.05 dam2, 33.71 nodes plant-1, and 138.8 g plant-1,) compared to the lowest values achieved by K0 comparison plants, which were 297.34 cm, 82.64 cm2, 30.04 nodes plant-1, and 118.4 g plant-1, respectively. The combination of boron and sorbitol had a significant effect on vegetative growth traits, as treatment T4 significantly outperformed and achieved the highest plant height, leaf area, number of nodes, and dry weight of the total vegetation, which were 317.91 cm, 99.12 dam2 plant, 35.032 nodes -plant-1, and 146.7 g plant-1, respectively, compared to the lowest values for vegetative growth traits, which were given by the plants of treatment T0, which were 285.32 cm, 76.95 cm2, 28.60 nodes plant-1, and 107.6 g plant-1, respectively.

Bioplastik merupakan material kemasan berbasis biomassa atau bahan terbarukan yang dapat digunakan untuk menanggulangi sampah plastik. Agar dan kitosan diketahui dapat disintesis menjadi bioplastik. Namun, karakteristik mekanik campuran agar dan kitosan masih belum sesuai dengan ekspektasi yakni memiliki nilai elongasi yang rendah. Plasticizer sangat dibutuhkan untuk memperbaiki karakteristik bioplastik. Penggunaan sorbitol sebagai plasticizer dalam pembuatan bioplastik berbasis agar dan kitosan hingga saat ini belum dilaporkan. Tujuan penelitian ini adalah menentukan konsentrasi sorbitol terbaik terhadap peningkatan karakteristik bioplastik berbasis agar dan kitosan. Metode yang dilakukan adalah mencampurkan sorbitol dengan konsentasi 0%, 1%, 1,25%, dan 1,5% (b/v) pada larutan utama bioplastik yaitu larutan agar 1% dan larutan kitosan 2%. Karakteristik bioplastik diketahui dengan melakukan uji ketebalan, kuat tarik, elongasi, daya serap air, gugus fungsi dengan FTIR, dan uji degradasi. Hasil penelitian ini menunjukkan bahwa konsentrasi sorbitol mempengaruhi karakteristik bioplastik. Formulasi bioplastik terpilih yaitu perlakuan penambahan sorbitol 1,25% dengan nilai ketebalan, daya serap air, kuat tarik, dan elongasi masing-masing sebesar 0,096±0,004 mm, 18,80±0,94%, 18,83±2,93 MPa, 95,89±12,62%. Bioplastik dengan 1,25% sorbitol membutuhkan waktu untuk terdegradasi sempurna selama 20 hari. Simpulan penelitian ini adalah karakteristik mekanis dari bioplastik berbasis kitosan dan agar dapat ditingkatkan dengan penambahan sorbitol 1,25% Abstract Bioplastic is a packaging material based on biomass or renewable materials that can be used to address plastic waste. Agar and chitosan are known to be able to be synthesized into bioplastic. However, the mechanical characteristics of agar and chitosan mixture are still not in line with expectations, particularly having low elongation values. Plasticizers are highly needed to improve the characteristics of bioplastics. The use of sorbitol as a plasticizer in the production of agar and chitosan-based bioplastics has not been reported to date. The aim of this research is to determine the optimal concentration of sorbitol for improving the characteristics of agar and chitosan-based bioplastics. The method involved mixing sorbitol at concentrations of 0%, 1%, 1.25%, and 1.5% (w/v) in the main bioplastic solution, which consists of 1% agar solution and 2% chitosan solution. The characteristics of the bioplastic were determined by conducting tests on thickness, tensile strength, elongation, water absorption, functional groups using FTIR, and degradation tests. The results of this study showed that the concentration of sorbitol affects the characteristics of bioplastics. The selected bioplastic formulation was the treatment with the addition of 1.25% sorbitol, with thickness, water absorption, tensile strength, and elongation values of 0.096±0.004 mm, 18.80±0.94%, 18.83±2.93 MPa, and 95.89±12.62%, respectively. Bioplastic with 1.25% sorbitol required 20 days to degrade completely. In conclusion, the mechanical characteristics of chitosan and agar-based bioplastics can be improved by the addition of 1.25% sorbitol.

Introduction: Carbohydrates, which make up 20 to 25% of tea beverages, are responsible for their flavor and bioactivity. Carbohydrates of pu-erh tea change during microbial fermentation and require further research. In this study, we examined the carbohydrate metabolism and expression of carbohydrateactive enzyme genes during the fermentation of tea leaves with Aspergillus luchuensis. Methods: Widely targeted metabolomics analysis, high-performance anionexchange chromatography measurements, and transcriptomics were used in this study. Results: After fermentation, the levels of soluble sugar, hemicellulose, lignin, eight monosaccharides, and seven sugar alcohols increased. Meanwhile, the relative contents of polysaccharides, D-sorbitol, D-glucose, and cellulose decreased. High expression of 40 genes encoding 16 carbohydrate enzymes was observed during fermentation (FPKM>10). These genes encode L-iditol 2-dehydrogenase, pectinesterase, polygalacturonase, α-amylase, glucoamylase, endoglucanase, β-glucosidase, β-galactosidase, α-galactosidase, α-glucosidase, and glucose-6-phosphate isomerase, among others. Discussion: These enzymes are known to break down polysaccharides and cell wall cellulose, increasing the content of monosaccharides and soluble sugars. [ABSTRACT FROM AUTHOR]

In this article, the synthesis of four novel bioelastomers known as poly (xylitol sebacate) (PXS), poly (xylitol adipate) (PXA), poly (sorbitol sebacate) (PSS), and poly (sorbitol adipate) (PSA) was carried out through bulk polymerization, followed by their blending using the solution method. Six blends were prepared namely PXS25/PSS75, PXS50/PSS50, PXS75/PSS25, PXS50/PXA50, PSA50/PXA50, and PSS50/PSA50. After blending, a thermal curing process was applied to the samples. The intrinsic viscosity, density, Tg, hydrophilicity, degradation, wound healing, and mechanical properties of polymers and blends were measured to investigate the effects of blending. Analyzing hardness, modulus, stress at breakpoint, degradation, and hydrophilicity data showed that the mechanical properties of bioelastomers are adjustable by blending for tissue engineering. Furthermore, the scratch assay proved the biocompatibility of pure polymers and indicated that blending does not have a negative impact on this matter. [ABSTRACT FROM AUTHOR]

Purpose: The present study aimed at evaluating possible synergistic effects between two risk factors for cognitive decline and neurodegenerative disorders, i.e. iron overload and exposure to a hypercaloric/hyperlipidic diet, on cognition, insulin resistance, and hippocampal GLUT1, GLUT3, Insr mRNA expression, and AKT phosporylation. Methods: Male Wistar rats were treated with iron (30 mg/kg carbonyl iron) or vehicle (5% sorbitol in water) from 12 to 14th post-natal days. Iron-treated rats received a standard laboratory diet or a high fat diet from weaning to adulthood (9 months of age). Recognition and emotional memory, peripheral blood glucose and insulin levels were evaluated. Glucose transporters (GLUT 1 and GLUT3) and insulin signaling were analyzed in the hippocampus of rats. Results: Both iron overload and exposure to a high fat diet induced memory deficits. Remarkably, the association of iron with the high fat diet induced more severe cognitive deficits. Iron overload in the neonatal period induced higher insulin levels associated with significantly higher HOMA-IR, an index of insulin resistance. Long-term exposure to a high fat diet resulted in higher fasting glucose levels. Iron treatment induced changes in Insr and GLUT1 expression in the hippocampus. At the level of intracellular signaling, both iron treatment and the high fat diet decreased AKT phosphorylation. Conclusion: The combination of iron overload with exposure to a high fat diet only led to synergistic deleterious effect on emotional memory, while the effects induced by iron and by the high fat diet on AKT phosphorylation were comparable. These findings indicate that there is, at least to some extent, an additive effect of iron combined with the diet. Further studies investigating the mechanisms associated to deleterious effects on cognition and susceptibility for the development of age-associated neurodegenerative disorders are warranted. [ABSTRACT FROM AUTHOR]

Pathogenic, biallelic variants in SORD were identified in 2020 as a novel cause for autosomal-recessive Charcot-Marie-Tooth disease (CMT) type 2, an inherited neuropathy. SORD codes for the enzyme sorbitol dehydrogenase. Loss of this enzyme's activity leads to an increase of sorbitol in serum. We retrospectively screened 166 patients with axonal neuropathy (predominantly CMT type 2, but including intermediate form of CMT and distal hereditary motor neuropathy (dHMN)) without identified genetic etiology for SORD mutations at a single large German neuromuscular center. Clinical and electrophysiology exam findings were analyzed for genotype–phenotype correlation. Five patients of the total cohort of 166 patients harbored pathogenic variants in SORD (3%). The homozygous frameshift variant c.757delG (p.Ala253Glnfs*27) was the most common (4/5). One additional case carried this variant on one allele only and an additional pathogenic missense variant c.458C > A (p.Ala153Asp) on the other allele. Age of onset ranged from early infancy to mid-twenties, and phenotypes comprised axonal CMT (4) and dHMN (1). Our findings strengthen the importance of screening for pathogenic variants in SORD, especially in patients with genetically unconfirmed axonal neuropathy, especially CMT type 2 and dHMN. [ABSTRACT FROM AUTHOR]

SUMMARY: Watercore is a common physiological disease of Rosaceae plants, such as apples (Malus domestica), usually occurring during fruit ripening. Apple fruit with watercore symptoms is prone to browning and rotting, thus losing commercial viability. Sorbitol and calcium ions are considered key factors affecting watercore occurrence in apples. However, the mechanism by which they affect the occurrence of watercore remains unclear. Here, we identified that the transcription factor MdWRKY9 directly binds to the promoter of MdSOT2, positively regulates the transcription of MdSOT2, increases sorbitol content in fruit, and promotes watercore occurrence. Additionally, MdCRF4 can directly bind to MdWRKY9 and MdSOT2 promoters, positively regulating their expression. Since calcium ions can induce the ubiquitination and degradation of the transcription factor MdCRF4, they can inhibit the transcription of MdWRKY9 and MdSOT2 by degrading MdCRF4, thereby reducing the sorbitol content in fruit and inhibiting the occurrence of fruit watercore disease. Our data sheds light on how calcium ions mitigate watercore in fruit, providing molecular‐level insights to enhance fruit quality artificially. [ABSTRACT FROM AUTHOR]

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A fully biobased waterborne flame‐retarded epoxy system was prepared using sorbitol polyglycidyl ether epoxy resin (SPE) and phytic acid (PA) as a reactive flame retardant (FR). The flame‐retardant efficiency was evaluated by comparing the reference SPE‐PA system with solventborne and waterborne SPE systems. Additional enhancement of intumescence and reduction of flammability was achieved by incorporating ammonium polyphosphate (APP) and melamine (MEL) into the SPE–PA system. PA, serving as a curing agent, contributed approximately 1% phosphorous content, resulting in an increased limiting oxygen index (LOI). UL‐94 flammability tests demonstrated improved FR properties with PA, and the addition of 2% phosphorous from APP to SPE‐PA achieved a self‐extinguishing V0 UL‐94 rating. Thermogravimetric analysis (TGA) revealed enhanced thermal stability and higher char yield with PA compared with other curing agents. Mass loss calorimetry (MLC) confirmed the superior charring effect of PA compared with other curing agents. The thermal insulation properties of the residual char were assessed by measuring the temperature on the back surface (Tb) of coated steel plates exposed to a 25 kW/m2 heat flux for 1 h. The PA sample containing 3%P of APP exhibited a Tb decrease of 130°C compared with the solventborne reference sample. Scanning electron microscopy analysis of the char morphology supported these findings, indicating the effectiveness of the intumescent FR system. Infrared spectra of the char residues and pyrolysis gaseous products were obtained to gain insights into the flame‐retardant mechanism of the different systems. [ABSTRACT FROM AUTHOR]

As people became more conscious of the risks associated with pollution in the early 21st century, eco‐friendly adhesives made from plant oil began to be used. Bio‐based adhesives are widely used due to their low price, easy accessibility, and high biodegradability; however, their low adhesive strength is an issue. This work incorporates three natural crosslinkers: tannic acid (TA), sorbitol (SR), and cellulose (CL) to address the drawbacks of bio‐based adhesives, such as low adhesive strength, inferior output in humidity and longer curing time that result from excess water. Methylene diphenyl diisocyanate (MDI) was used to react with soybean oil‐based polyol (SOP) plus the crosslinkers to make bio‐based polyurethane adhesives. The maximum bonding strength of the soybean‐based adhesive containing 10 wt.% of SR (SR‐10%) was measured at 6.19 MPa which is about 44% higher than the sample without SR. The higher strength is due to the crosslinking ability of SR. The effect of crosslinking is also observed in the study of polar protic and polar aprotic solvents used to dissolve the adhesive. As compared with the polar aprotic solvent tetrahydrofuran, TA‐containing adhesive samples showed a maximum tensile strength of 3.69 MPa in methanol; a polar protic solvent that can participate in further hydrogen bonding to form a higher crosslinked complex. The glass transition temperature (Tg) increases with the increase in crosslinker amount. The observed increase in Tg may be attributable to an increase in crosslinking density, which reduces polymer chain flexibility and causes a stronger material. Highlights: Bio‐adhesives based on soybean oil and diisocyanate were synthesized.Natural crosslinking agents such as tannic acid, sorbitol, and cellulose were used.The effect of crosslinking using polar protic and aprotic solvents was studied.The glass transition temperature increases with the increase in crosslinker amount. [ABSTRACT FROM AUTHOR]

Objective: To obtain protocols for clonal micropropagation, crop planning, and in vitro conservation of Rhycholaelia digbyana (Lindley) Schltr. Methodology: The effects of the Kundson C basal medium and benzylaminopurine concentration were evaluated for clonal micropropagation. The treatment with the greatest number of shoots formed per apex was selected for crop planning. Experiments were conducted to determine the effect of basal medium Murashige and Skoog concentration at 2.2 gL-1 and 4.4 gL-1; sorbitol, mannitol, and sucrose at 1, 2, and 3% on slow growth. Results: The best treatment for clonal micropropagation and crop planning was identified as 21.60 gL-1 Knudson C with 8.80 µM benzylaminopurine. This treatment resulted in uniform-sized shoots produced. The multiplication process can yield 10,240 seedlings in 12 months. Slow growth was achieved using Murashige and Skoog basal media at 2.2 gL-1 with 1% mannitol. Implications: More experiments must be conducted to determine the best shoot induction conditions and improve resource efficiency. Conclusions: These findings represent the first report on micropropagation and ex-situ conservation to preserve germplasm for this species as an important resource for the floriculture industry. [ABSTRACT FROM AUTHOR]

The polyol pathway is a primary mechanism for the formation of sorbitol, and its accumulation is a major factor in many diseases' complications. Importantly, there have previously been some indications that there is a relationship between polyol intermediates and thyroid hormones in hyperthyroidism state. Hence, this study aimed to shed light on the therapeutic impact of nano-quercetin (QT-NPs) on hyperthyroidism via inhibiting the polyol pathway. Besides control groups, hyperthyroidism was triggered in the rats by 100 μg/kg of l-thyroxin and further treated with quercetin (QT) and nano-quercetin (10 mg/kg body weight). Additionally, levels of thyroid hormones (T3 and T4), oxidative markers, and the antioxidant system were monitored. Besides histological investigation of thyroid gland, the level of aldose reductase was estimated by qPCR. The obtained findings indicated an elevation of T3 and T4 which indicated hyperthyroidism. This hyperthyroid state was accompanied by an elevation in oxidative stress and a decline in antioxidant status. Importantly, the expression level of aldose reductase was elevated, as was the level of sorbitol. The treatment with QT and QT-NPs potentially reduced the expression level of aldose reductase and the concentration of sorbitol, indicating the inhibition capability of QT and QT-NPs on the polyol pathway key enzyme. Furthermore, rat groups treated with QT and QT-NPs showed an elevation in the antioxidant system (GSH, GPx) with a notable decline in the oxidative stress marker MDA. Furthermore, histopathological examination of the thyroid tissue of hyperthyroidism group treated with QT-NPs confirms the potential effect of QT-NPs on the thyroid tissue. In conclusion, QT-NPs showed therapeutic superiority over QT in hyperthyroidism treatment through inhibition of aldose reductase, which is the key enzyme in the polyol pathway. [ABSTRACT FROM AUTHOR]

Contaminated lake water and fish can be sources of bacterial pathogens of public health concern, including pathogenic E. coli. Within Ethiopia, specifically, Central Oromia, raw fish consumption is a common practice. Although there are few reports on occurrence of E. coli O157 in fish destined for human consumption and children under five years, information on the transmission pathways of E. coli O157 and other sorbitol non-fermenting (SN-F) E. coli from water-to-fish-to-human, and their virulence factors and antimicrobial resistant determinants along the fish supply chain is lacking. The study aimed to investigate the occurrence, molecular characteristics, and antimicrobial susceptibility of E. coli O157 and other SN-F E. coli strains in fish, lake water and humans in central Oromia, Ethiopia. A total of 750 samples (450 fish samples, 150 water samples, 150 human stool samples) were collected from five lakes and three health facilities. The samples were processed following the standard protocol recommended by European Food Safety Authority and Kirby–Bauer disc diffusion method for detection of the bacteria, and antimicrobial susceptibility tests, respectively. Molecular characterization of presumptive isolates was performed using Whole-Genome Sequencing (WGS) for serotyping, determination of virulence factors, antimicrobial resistance traits, and genetic linkage of the isolates. Overall, 3.9% (29/750) of the samples had SN-F E. coli; of which 6.7% (n = 10), 1.8% (n = 8) and 7.3% (n = 11) were retrieved from water, fish, and diarrheic human patients, respectively. The WGS confirmed that all the isolates were SN-F non-O157: H7 E. coli strains. We reported two new E. coli strains with unknown O-antigen from fish and human samples. All the strains have multiple virulence factors and one or more genes encoding for them. Genetic relatedness was observed among strains from the same sources (water, fish, and humans). Most isolates were resistant to ampicillin (100%), tetracycline (100%), cefotaxime (100%), ceftazidime (100%), meropenem (100%), nalidixic acid (93.1%) and sulfamethoxazole/trimethoprim (79.3%). Majority of the strains were resistant to chloramphenicol (58.6%) and ciprofloxacin (48.3%), while small fraction showed resistance to azithromycin (3.45%). Isolates had an overall MDR profile of 87.5%. Majority, (62.1%; n = 18) of the strains had acquired MDR traits. Genes encoding for mutational resistance and Extended-spectrum beta-lactamases (ESBL) were also detected. In conclusion, our study revealed the occurrence of virulent and MDR SN-F E. coli strains in water, fish, and humans. Although no genetic relatedness was observed among strains from various sources, the genomic clustering among strains from the same sources strongly suggests the potential risk of transmission along the supply chain at the human–fish-environment interface if strict hygienic fish production is not in place. Further robust genetic study of the new strains with unknown O-antigens, and the epidemiology of SN-F E. coli is required to elucidate the molecular profile and public health implications of the pathogens. [ABSTRACT FROM AUTHOR]

This study prepares biodegradable biofilms for packaging applications from biomass to help reduce plastic waste while also adding value to biomass. Furthermore, the effects of plasticization with 30 wt% plasticizer (relative to the mass of cassava starch) by glycerol (G), sorbitol (S), and a mixture of glycerol and sorbitol (GS) plasticizers are investigated, and the effects of bio‐SiO2 particles extracted from sugarcane leave via the sol–gel method are evaluated in terms of the mechanical and biodegradability properties of the cassava starch‐based biofilms. Biofilms used for packing applications must be insoluble and exhibit good elongation properties. In this context, the results indicate that the GS mixture produces a biofilm that exhibits these desirable properties. Thus, cassava starch‐based biofilms are prepared using GS and varying concentrations of the bio‐SiO2 particles (i.e., 0, 0.5, 1, and 1.5 wt%). The films containing bio‐SiO2 exhibit significantly lower water solubilities and moisture contents than the corresponding bio‐SiO2‐free films. Additionally, the inclusion of bio‐SiO2 particles increases the water resistance properties of the biofilms. Overall, the results suggest that cassava starch films prepared using a mixture of plasticizers and reinforced with bio‐SiO2 particles can be considered a safe material for application in biodegradable food packaging. [ABSTRACT FROM AUTHOR]

Glycols, such as 1,2-propanediol (1,2-PDO) and ethylene glycol (EG), are considered as a kind of essential intermediates for producing high-value fine chemicals. The direct conversion of biomass derivatives into glycols is still a great challenge for the chemical industry. In this study, we developed an efficient and green process for synthesizing bimetallic Ru/WOx modified biomass-derived N-doped carbon catalysts (Ru/WNC) by tandem hydrothermal carbonization of bamboo shoot, thermal pyrolysis and hydrothermal reduction process. The structure, surface properties, and catalytic activity of Ru/WNC catalyst for sorbitol hydrogenolysis to 1,2-PDO and EG in an aqueous alkaline solution with Ca(OH)2 as base promoter were studied in detail. Results displayed that N incorporation would lead to ligand effects by combining with the active metal Ru and WOx. The introduction of WOx would promote Ru nanoparticles with small size (~ 2.4 nm) well distributed on the carbon surface, as well as improve the capability of hydrogen spillover. Meanwhile, Ca(OH)2 and rich basic W5+ sites on Ru/WNC would promote the cleavage of the C–C bonds by the retro-aldol condensation. As a result, the bimetallic catalyst has demonstrated superior hydrogenolysis activity for 1,2-PDO and EG formation in a basic medium. The highest sorbitol conversion (75.4%) and the total selectivity of 1,2-PDO and EG (84.6%) were achieved by the cooperative action of metallic Ru and WOx species. The possible reaction mechanism of Ru/WNC in the basic medium was also discussed. This study provides informative principles for the design of efficient hydrogenolysis catalysts for biomass-derived glycols production. [ABSTRACT FROM AUTHOR]

In this work, soluble and low-molecular-weight phenolic resol was cross-linking solidified by a solvothermal method using hexamethylenetetramine as a curing agent, which was modified with hydrophobic functional groups and sulfonated by chlorosulfonic acid. Modified and sulfonated phenolic resins were used as catalysts for the dehydration of sorbitol to isosorbide, and the effects of surface hydrophobicity, acid type and acid amount on sorbitol conversion and isosorbide yield were investigated systematically. The results showed that the introduction of 3-MPTMS by coupling with phenolic hydroxyl groups of phenolic resins can significantly enhance the surface hydrophobicity of sulfonated phenolic resins and increase the surface acid amount by the oxidation of thiol groups in 3-MPTMS using H2O2 to sulfonic acid groups. As a result, the catalytic performance of sulfonated phenolic resins with surface hydrophobicity modification was improved obviously; about 100% sorbitol conversion and 75.44% isosorbide yield could be achieved under optimal reaction conditions. Compared to commercial acid resins, sulfonated phenolic resins offer the advantages of lower cost and a more convenient preparation process. [ABSTRACT FROM AUTHOR]

A series of nickel hydroxyapatite catalysts were synthesized by the co‐precipitation method followed by calcination and reduction. These catalysts were employed for the aqueous phase hydrogenation of glucose to sorbitol. The Ni‐HAP catalyst with comparatively high surface area and acid‐base strength gave high sorbitol selectivity in 1 h. Ni‐HAP‐4 catalyst with moderate Ni (3.5 wt. %) content having smaller and highly dispersed nickel particles gives an excellent yield of sorbitol, 97 % in 1 h. The Ni‐HAP‐4 catalyst works well with other polar protic solvents. Different characterization techniques like XRD, TEM, SEM‐EDS, BET, NH3‐TPD, and CO2‐TPD were employed to analyze the Ni‐HAP‐4 catalyst. [ABSTRACT FROM AUTHOR]

The possible production of fructo-oligosaccharides from sucrose was evaluated in the Base Business Unit (UEB) "Ignacio Agramonte'", which currently produces sorbitol, with low exploitation of the installed capacity, due to lack of glucose. There was an opportunity in infrastructure, time and labor to convert the entity into a multipurpose plant. A simulation model of the technology for the production of fructo-oligosaccharide syrup from sucrose was developed, based on the enzymatic transformation of sucrose into fructo-oligosaccharides, using the recombinant enzyme 1-SST (1-sucrose: sucrose fructosyltransferase) expressed in Pichia pastoris, obtained at the Center for Genetic Engineering and Biotechnology at Sancti Spíritus. Model validation yielded relative errors lesser than 5 %. Two improvement alternatives were evaluated based on increasing the production of FOS by using the actual installed capacity in the sorbitol factory (alternative 1) and also increasing the initial concentration of the sucrose solution (alternative 2). Feasibility of the alternatives was evaluated by comparing the annual net cash income (Anci) and expenses. Alternative 2 was the best, producing 841.5 t of fructo-oligosaccharides in 90 working days, with a benefit/cost ratio of 1,2. [ABSTRACT FROM AUTHOR]

The systemic effects of the artificial sweetener sorbitol on older adult individuals have not been elucidated. We assessed the effects of sorbitol consumption on cognitive and gingival health in a mouse model. Aged mice were fed 5% sorbitol for 3 months before their behavior was assessed, and brain and gingival tissues were collected. Long‐term sorbitol consumption inhibited gingival tissue aging in aged mice. However, it caused cognitive decline and decreased brain‐derived neurotrophic factor (BDNF) in the hippocampus. Sorbitol consumption did not affect homeostatic function; however, it may exert effects within the brain, particularly in the hippocampus. [ABSTRACT FROM AUTHOR]

Objective. Microfold cells (M cells) are specific intestinal epithelial cells for monitoring and transcytosis of antigens, microorganisms, and pathogens in the intestine. However, the mechanism for M-cell development remained elusive. Materials and Methods. Real-time polymerase chain reaction, immunofluorescence, and western blotting were performed to analyze the effect of sorbitol-regulated M-cell differentiation in vivo and in vitro, and luciferase and chromatin Immunoprecipitation were used to reveal the mechanism through which sorbitol-modulated M-cell differentiation. Results. Herein, in comparison to the mannitol group (control group), we found that intestinal M-cell development was inhibited in response to sorbitol treatment as evidenced by impaired enteroids accompanying with decreased early differentiation marker Annexin 5, Marcksl1, Spib, sox8, and mature M-cell marker glycoprotein 2 expression, which was attributed to downregulation of receptor activator of nuclear factor kappa-В ligand (RANKL) expression in vivo and in vitro. Mechanically, in the M-cell model, sorbitol stimulation caused a significant upregulation of phosphodiesterase 4 (PDE4) phosphorylation, leading to decreased protein kinase A (PKA)/cAMP-response element binding protein (CREB) activation, which further resulted in CREB retention in cytosolic and attenuated CREB binds to RANKL promoter to inhibit RANKL expression. Interestingly, endogenous PKA interacted with CREB, and this interaction was destroyed by sorbitol stimulation. Most importantly, inhibition of PDE4 by dipyridamole could rescue the inhibitory effect of sorbitol on intestinal enteroids and M-cell differentiation and mature in vivo and in vitro. Conclusion. These findings suggested that sorbitol suppressed intestinal enteroids and M-cell differentiation and matured through PDE4-mediated RANKL expression; targeting to inhibit PDE4 was sufficient to induce M-cell development. [ABSTRACT FROM AUTHOR]

Processes of deformation and fracture of a natural ferrite-martensite composite under a local high-speed impact are considered. Targets fabricated from steel 14G2 with an isotropic structure of tempered sorbite and from the composite are studied after impact tests. It is shown that the target from steel 14G2 with sorbite structure is pierced through under the concentrated high-power impact of a bullet. When the target is made of the composite, the bullet ricochets. The mechanisms of the processes occurring in the targets under the impact of a bullet are analyzed. [ABSTRACT FROM AUTHOR]

The stability of monoclonal antibodies (mAbs) is vital for their therapeutic success. Sorbitol, a common mAb stabilizer used to prevent aggregation, was evaluated for any potential adverse effects on the chemical stability of mAb X. An LC-MS/MS based analysis focusing on the post-translational modifications (PTMs) of mAb X was conducted on samples that had undergone accelerated aging at 40°C. Along with PTMs that are known to affect mAbs' structure function and stability (such as deamidation and oxidation), a novel mAb PTM was discovered, the esterification of glutamic acid by sorbitol. Incubation of mAb X with a 1:1 ratio of unlabeled sorbitol and isotopically labeled sorbitol (13C6) further corroborated that the modification was the consequence of the esterification of glutamic acid by sorbitol. Levels of esterification varied across glutamic acid residues and correlated with incubation time and sorbitol concentration. After 4 weeks of accelerated stability with isotopically labeled sorbitol, it was found that 16% of the total mAb possesses an esterified glutamic acid. No esterification was observed at aspartic acid sites despite the free carboxylic acid side chain. This study unveils a unique modification of mAbs, emphasizing its potential significance for formulation and drug development. [ABSTRACT FROM AUTHOR]

The co-administration of curcumin and hesperetin might be beneficial in terms of neuroprotective activity; therefore, in this study, we attempted to develop a fixed-dose formulation comprising these two compounds in an amorphous state. The aim of obtaining an amorphous state was to overcome the limitations of the low solubility of the active compounds. First, we assessed the possibility of using popular sweeteners (erythritol, xylitol, and sorbitol) as plasticizers to reduce the glass transition temperature of PVP K30 to prepare the polymer–excipient blends, which allowed the preparation of amorphous solid dispersions via hot-melt extrusion at a temperature below the original glass transition of PVP K30. Erythritol proved to be the superior plasticizer. Then, we focused on the development of fixed-dose amorphous solid dispersions of curcumin and hesperetin. Powder X-ray diffraction and thermal analysis confirmed the amorphous character of dispersions, whereas infrared spectroscopy helped to assess the presence of intermolecular interactions. The amorphous state of the produced dispersions was maintained for 6 months, as shown in a stability study. Pharmaceutical parameters such as dissolution rate, solubility, and in vitro permeability through artificial membranes were evaluated. The best improvement in these features was noted for the dispersion, which contained 15% of the total content of the active compounds with erythritol used as the plasticizer. [ABSTRACT FROM AUTHOR]

Microalgal biomass can be utilized for the production of value-added chemicals and fuels. Within this research, Chlorella vulgaris biomass left behind after the extraction of lipids and proteins was converted to valuable sugars, organic acids and furanic compounds via hydrolysis/dehydration using dilute aqueous sulfuric acid as a homogeneous catalyst. Under mild conditions, i.e., low temperature and low sulfuric acid concentration, the main products of hydrolysis/dehydration were monomeric sugars (glucose and xylose) and furanic compounds (HMF, furfural) while under more intense conditions (i.e., higher temperature and higher acid concentration), organic acids (propionic, formic, acetic, succinic, lactic, levulinic) were also produced either directly from sugar conversion or via intermediate furans. As a second valorization approach, the residual microalgal biomass was converted to value-added sugar alcohols (sorbitol, glycerol) via hydrogenation/hydrogenolysis reactions over metallic ruthenium catalysts supported on activated carbons (5%Ru/C). It was also shown that a low concentration of sulfuric acid facilitated the conversion of biomass to sugar alcohols by initiating the hydrolysis of carbohydrates to monomeric sugars. Overall, this work aims to propose valorization pathways for a rarely utilized residual biomass towards useful compounds utilized as platform chemicals and precursors for the production of a wide variety of solvents, polymers, fuels, food ingredients, pharmaceuticals and others. [ABSTRACT FROM AUTHOR]