1. Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405 : comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation
- Author
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Duncan J. Maskell, Thomas J. Inzana, Sonia Lacouture, Marc Stegger, Marcelo Gottschalk, Alexander W. Tucker, Liancheng Lei, Janine T. Bossé, Miriam Koene, Øystein Angen, David Harris, Paul R. Langford, Aloka B. Bandara, Yanwen Li, Matthew T. G. Holden, Liza Miriam Cohen, Olusegun Oshota, Peter Kuhnert, Brendan W. Wren, Andrew N. Rycroft, University of St Andrews. School of Medicine, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. St Andrews Bioinformatics Unit, University of St Andrews. Infection and Global Health Division, Biotechnology and Biological Sciences Research Council (BBSRC), and Biotechnology and Biological Sciences Research Council
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Swine ,BRa ,Genome ,Actinobacillus Infections ,Plasmid ,serovar 8 reference genome ,AA, amino acid [SNPs. Abbreviations] ,Swine Diseases ,Genetics ,biology ,630 Agriculture ,Strain (biology) ,Bacteriologie ,Actinobacillus pleuropneumoniae ,High-Throughput Nucleotide Sequencing ,Bacteriology, Host Pathogen Interaction & Diagnostics ,QR Microbiology ,General Medicine ,ATCC ,BHI ,amino acid ,0605 Microbiology ,Brain Heart Infusion ,SNPs ,Short Communications ,QH426 Genetics ,Pathogens and Epidemiology ,Serogroup ,Serovar 8 reference genome ,American Type Culture Collection ,Genetic variation ,Animals ,QH426 ,Gene ,Illumina dye sequencing ,Host Pathogen Interaction & Diagnostics ,Whole genome sequencing ,0604 Genetics ,ATCC, American Type Culture Collection ,Genetic Variation ,DAS ,Bacteriology ,BHI, Brain Heart Infusion ,SNPs. Abbreviations: AA ,biology.organism_classification ,Host Pathogen Interactie & Diagnostiek ,SNPs. Abbreviations: AA, amino acid ,QR ,Bacteriologie, Host Pathogen Interactie & Diagnostiek ,570 Life sciences ,Genome, Bacterial - Abstract
This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, BB/G018553/1, BB/S002103/1, and BB/S005897/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. Funding for LL provided by the ‘National Natural Science Foundation of China’ (No.31520103917). MTGH and DH were supported by the Wellcome Trust (grant number 098051). We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain. Publisher PDF
- Published
- 2021
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