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350 results on '"SERGEANT, KJELL"'

8. The impact of dietary Laminaria digitata and alginate lyase supplementation on the weaned piglet liver: A comprehensive proteomics and metabolomics approach

Author

Ribeiro, David M., primary, Leclercqc, Celine C., additional, Charton, Sophie A.B., additional, Costa, Mónica M., additional, Carvalho, Daniela F.P., additional, Sergeant, Kjell, additional, Cocco, Emmanuelle, additional, Renaut, Jenny, additional, Freire, João P.B., additional, Prates, José A.M., additional, and de Almeida, André M., additional

Published
2023
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13. Isoprene-Emitting Tobacco Plants Are Less Affected by Moderate Water Deficit under Future Climate Change Scenario and Show Adjustments of Stress-Related Proteins in Actual Climate

Author

Pollastri, Susanna, primary, Velikova, Violeta, additional, Castaldini, Maurizio, additional, Fineschi, Silvia, additional, Ghirardo, Andrea, additional, Renaut, Jenny, additional, Schnitzler, Jörg-Peter, additional, Sergeant, Kjell, additional, Winkler, Jana Barbro, additional, Zorzan, Simone, additional, and Loreto, Francesco, additional

Published
2023
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22. Characterization of MdMYB68, a suberin master regulator in russeted apples.

Author

Xuan Xu, Guerriero, Gea, Domergue, Frederic, Beine-Golovchuk, Olga, Cocco, Emmanuelle, Berni, Roberto, Sergeant, Kjell, Hausman, Jean-Francois, and Legay, Sylvain

Subjects
GENE expression profiling, NICOTIANA benthamiana, GENE expression, FRUIT skins, TRANSCRIPTION factors, LIPID analysis
Abstract

Introduction: Apple russeting is mainly due to the accumulation of suberin in the cell wall in response to defects and damages in the cuticle layer. Over the last decades, massive efforts have been done to better understand the complex interplay between pathways involved in the suberization process in model plants. However, the regulation mechanisms which orchestrate this complex process are still under investigation. Our previous studies highlighted a number of transcription factor candidates from the Myeloblastosis (MYB) transcription factor family which might regulate suberization in russeted or suberized apple fruit skin. Among these, we identified MdMYB68, which was co-expressed with number of well-known key suberin biosynthesis genes. Method: To validate the MdMYB68 function, we conducted an heterologous transient expression in Nicotiana benthamiana combined with whole gene expression profiling analysis (RNA-Seq), quantification of lipids and cell wall monosaccharides, and microscopy. Results: MdMYB68 overexpression is able to trigger the expression of the whole suberin biosynthesis pathway. The lipid content analysis confirmed that MdMYB68 regulates the deposition of suberin in cell walls. Furthermore, we also investigated the alteration of the non-lipid cell wall components and showed that MdMYB68 triggers a massive modification of hemicelluloses and pectins. These results were finally supported by the microscopy. Discussion: Once again, we demonstrated that the heterologous transient expression in N. benthamiana coupled with RNA-seq is a powerful and efficient tool to investigate the function of suberin related transcription factors. Here, we suggest MdMYB68 as a new regulator of the aliphatic and aromatic suberin deposition in apple fruit, and further describe, for the first time, rearrangements occurring in the carbohydrate cell wall matrix, preparing this suberin deposition. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Dereper, LC-MS only once. When an identification is forever

Author

Zorzan, Simone, Sergeant, Kjell, and Charton, Sophie

Abstract

The untargeted knowledge on metabolites present in samples would provide essential information in running projects. However, standardization, throughput and depth of analysis for metabolites are low mainly because metabolite identification is slow and driven by manual interpretation of mass spectra. Although targeted metabolite analyses allow for higher throughput and improved standardization, they do not give an as exhaustive view on sample composition as untargeted metabolite analyses. Dereplication, the use of previous in-house data to identify compounds, would speed up this process, thereby liberating time to identify “new” compounds. The objective of DEREPer is to provide a workflow for the dereplication of metabolite identification in LC/MS-MS. This software implements a database for compounds memorization to store details such as name, formula, M/Z, protocol, project, organism, references, peak list etc. and to store data for the matches of an already known compound across experiments. A graphical user interface allows to input and edit a compound or a match to an existing one. Peak list can be visualized. When a new compound has the same formula as an existing one, the user can thus verify if the two compounds are the same or not. Data can be exported in binary format, or in the standard .msp text format, for compatibility with external software. DEREPer overcomes the “identification bottleneck”, and metabolite identification will be quicker. More and more diverse metabolites will be identified, making metabolomics a high-throughput approach. Compliance with internal and external demands for rapid responses will strengthen the metabolomics pipelines performances.

Published
2022
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24. Multiplicity of aspartic proteinases from Cynara cardunculus L.

Author

Sergeant, Kuell, Sarmento, Ana Cristina, Lopes, Henrique, Oliveira, Cláudia S., Vitorino, Rui, Samyn, Bart, Sergeant, Kjell, Debyser, Griet, Van Beeumen, Jozef, Domingues, Pedro, Amado, Francisco, Pires, Euclides, Domingues, M. Rosário M., and Barros, Marlene T.

Published
2009

31. The Roots of Plant Frost Hardiness and Tolerance

Author

AMBROISE, Valentin, Legay, Sylvain, Guerriero, Gea, Hausman, Jean-Francois, CUYPERS, Ann, Sergeant, Kjell, Sergeant, Kjell, Guerriero, Gea, Hausman, Jean-Francois, CUYPERS, Ann, Legay, Sylvain, and AMBROISE, Valentin

Subjects
Frost, Hardiness, Cold acclimation, Abiotic stress, Roots, Tolerance, eye diseases
Abstract

Frost stress severely affects agriculture and agroforestry worldwide. Although many studies about frost hardening and resistance have been published, most of them focused on the aboveground organs and only a minority specifically targets the roots. However, roots and aboveground tissues have different physiologies and stress response mechanisms. Climate models predict an increase in the magnitude and frequency of late-frost events, which, together with an observed loss of soil insulation, will greatly decrease plant primary production due to damage at the root level. Molecular and metabolic responses inducing root cold hardiness are complex. They involve a variety of processes related to modifications in cell wall composition, maintenance of the cellular homeostasis and the synthesis of primary and secondary metabolites. After a summary of the current climatic models, this review details the specificity of freezing stress at the root level and explores the strategies roots developed to cope with freezing stress. We then describe the level to which roots can be frost hardy, depending on their age, size category and species. After that, we compare the environmental signals inducing cold acclimation and frost hardening in the roots and aboveground organs. Subsequently, we discuss how roots sense cold at a cellular level and briefly describe the following signal transduction pathway, which leads to molecular and metabolic responses associated with frost hardening. Finally, the current options available to increase root frost tolerance are explored and promising lines of future research are discussed. Luxembourg National Research Fund (FNR) [project X-press AFR PhD/17/SR 11634190]

Published
2020

35. MdMYB52 regulates lignin biosynthesis upon the suberization process in apple.

Author

Xuan Xu, Guerriero, Gea, Berni, Roberto, Sergeant, Kjell, Guignard, Cedric, Lenouvel, Audrey, Hausman, Jean-Francois, and Legay, Sylvain

Subjects
LIGNINS, BIOSYNTHESIS, ANALYTICAL chemistry, METABOLISM, ARABIDOPSIS thaliana, NICOTIANA benthamiana
Abstract

Our previous studies, comparing russeted vs. waxy apple skin, highlighted a MYeloBlastosys (Myb) transcription factor (MdMYB52), which displayed a correlation with genes associated to the suberization process. The present article aims to assess its role and function in the suberization process. Phylogenetic analyses and research against Arabidopsis thaliana MYBs database were first performed and the tissue specific expression of MdMYB52 was investigated using RT-qPCR. The function of MdMYB52 was further investigated using Agrobacterium-mediated transient overexpression in Nicotiana benthamiana leaves. An RNA-Seq analysis was performed to highlight differentially regulated genes in response MdMYB52. Transcriptomic data were supported by analytical chemistry and microscopy. A massive decreased expression of photosynthetic and primary metabolism pathways was observed with a concomitant increased expression of genes associated with phenylpropanoid and lignin biosynthesis, cell wall modification and senescence. Interestingly key genes involved in the synthesis of suberin phenolic components were observed. The analytical chemistry displayed a strong increase in the lignin content in the cell walls during MdMYB52 expression. More specifically, an enrichment in G-Unit lignin residues was observed, supporting transcriptomic data as well as previous work describing the suberin phenolic domain as a G-unit enriched lignin-like polymer. The time-course qPCR analysis revealed that the observed stress response, might be explain by this lignin biosynthesis and by a possible programmed senescence triggered by MdMYB52. The present work supports a crucial regulatory role for MdMYB52 in the biosynthesis of the suberin phenolic domain and possibly in the fate of suberized cells in russeted apple skins. [ABSTRACT FROM AUTHOR]

Published
2022
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36. A comparative proteomic analysis of human umbilical vein endothelial cells after infection with the rodentborne hemorrhagic fevers puumala hantavirus and Leptospira interrogans serovar Copenhageni

Author

Goeijenbier, Marco, primary, Martina, Byron E.E., additional, Goris, Marga G.A., additional, Ahmed, Ahmed, additional, Hartskeerl, Rudy, additional, Planchon, Sebastién, additional, Sergeant, Kjell, additional, Renaut, Jenny, additional, van Gorp, Eric C.M., additional, Nally, Jarlath, additional, and Schuller, Simone, additional

Published
2014
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39. Molecular insights into plant desiccation tolerance: transcriptomics, proteomics and targeted metabolite profiling in Craterostigma plantagineum

Author

Xu, Xuan, primary, Legay, Sylvain, additional, Sergeant, Kjell, additional, Zorzan, Simone, additional, Leclercq, Céline C., additional, Charton, Sophie, additional, Giarola, Valentino, additional, Liu, Xun, additional, Challabathula, Dinakar, additional, Renaut, Jenny, additional, Hausman, Jean‐Francois, additional, Bartels, Dorothea, additional, and Guerriero, Gea, additional

Published
2021
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41. Long-Term Cd Exposure Alters the Metabolite Profile in Stem Tissue of Medicago sativa

Author

UCL - SST/ELI/ELIA - Agronomy, Gutsch, Annelie, Hendrix, Sophie, Guerriero, Gea, Renaut, Jenny, Lutts, Stanley, Alseekh, Saleh, Fernie, Alisdair R., Hausman, Jean-Francois, Vangronsveld, Jaco, Cuypers, Ann, Sergeant, Kjell, UCL - SST/ELI/ELIA - Agronomy, Gutsch, Annelie, Hendrix, Sophie, Guerriero, Gea, Renaut, Jenny, Lutts, Stanley, Alseekh, Saleh, Fernie, Alisdair R., Hausman, Jean-Francois, Vangronsveld, Jaco, Cuypers, Ann, and Sergeant, Kjell

Abstract

As a common pollutant, cadmium (Cd) is one of the most toxic heavy metals accumulating in agricultural soils through anthropogenic activities. The uptake of Cd by plants is the main entry route into the human food chain, whilst in plants it elicits oxidative stress by unbalancing the cellular redox status. Medicago sativa was subjected to chronic Cd stress for five months. Targeted and untargeted metabolic analyses were performed. Long-term Cd exposure altered the amino acid composition with levels of asparagine, histidine and proline decreasing in stems but increasing in leaves. This suggests tissue-specific metabolic stress responses, which are often not considered in environmental studies focused on leaves. In stem tissue, profiles of secondary metabolites were clearly separated between control and Cd-exposed plants. Fifty-one secondary metabolites were identified that changed significantly upon Cd exposure, of which the majority are (iso)flavonoid conjugates. Cadmium exposure stimulated the phenylpropanoid pathway that led to the accumulation of secondary metabolites in stems rather than cell wall lignification. Those metabolites are antioxidants mitigating oxidative stress and preventing cellular damage. By an adequate adjustment of its metabolic composition, M. sativa reaches a new steady state, which enables the plant to acclimate under chronic Cd stress.

Published
2020

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