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280 results on '"SENSEBÉ, LUC"'

1. Safety and preliminary efficacy of allogeneic bone marrow-derived multipotent mesenchymal stromal cells for systemic sclerosis: a single-centre, open-label, dose-escalation, proof-of-concept, phase 1/2 study

Author

Farge, Dominique, Loisel, Séverine, Resche-Rigon, Matthieu, Lansiaux, Pauline, Colmegna, Ines, Langlais, David, Charles, Catney, Pugnet, Grégory, Maria, Alexandre Thibault Jacques, Chatelus, Emmanuel, Martin, Thierry, Hachulla, Eric, Kheav, Vissal David, Lambert, Nathalie C, Wang, HanChen, Michonneau, David, Martinaud, Christophe, Sensebé, Luc, Cras, Audrey, and Tarte, Karin

Published
2022
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2. Safety, tolerability, and activity of mesenchymal stem cells versus placebo in multiple sclerosis (MESEMS): a phase 2, randomised, double-blind crossover trial

Author

Aghdami, Naser, Agüera-Morales, Eduardo, Ali, Rehiana, Allan, David, Arab, Leila, Battaglia, Mario, Berry, Isabelle, Blinkenberg, Morten, Bonetti, Bruno, Brundin, Lou, Capelli, Chiara, Castellan, Lucio, Cellerino, Maria, Cencioni, Maria Teresa, Clanet, Michel, Comi, Giancarlo, Courtman, David, Dazzi, Francesco, Fischer-Nielsen, Anne, Fernandez, Oscar, Fernandez, Victoria, Freedman, Mark S., Furlan, Roberto, Gimona, Mario, Gualandi, Francesca, Guan, Qingdong, Iacobaeus, Ellen, Inglese, Matilde, Introna, Martino, Izquierdo, Guillermo, Karimi, Shahedeh, Laroni, Alice, Le Blanc, Katarina, Loaiza, Sandra, Mallik, Shahrukh, Marley, Stephen, Marrie, Ruth Ann, Marriot, James, Martino, Gianvito, Nabavi, Seyed Massood, Miller, David, Muraro, Paolo A., Nicholas, Richard, Oliveri, Roberto S, Orengo, Giovanni, Palanicawande, Renuka, Pardini, Matteo, Radue, Ernst W, Ramo Tello, Cristina, Rush, Carolina, Schiavetti, Irene, Sellner, Johann, Sensebe, Luc, Soelberg Sorensen, Per, Sormani, Maria Pia, Strunk, Dirk, Szwajcer, David, Thalamas, Claire, Uccelli, Antonio, Wuerfel, Jens Thomas, Battaglia, Mario Alberto, Marriott, James, Muraro, Paolo, Radue, Ernst, Sorensen, Per Soelberg, and Freedman, Mark S

Published
2021
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3. In vitro bone metastasis dwelling in a 3D bioengineered niche

Author

Han, Weijing, El Botty, Rania, Montaudon, Elodie, Malaquin, Laurent, Deschaseaux, Frederic, Espagnolle, Nicolas, Marangoni, Elisabetta, Cottu, Paul, Zalcman, Gérard, Parrini, Maria Carla, Assayag, Franck, Sensebe, Luc, Silberzan, Pascal, Vincent-Salomon, Anne, Dutertre, Guillaume, Roman-Roman, Sergio, Descroix, Stephanie, and Camonis, Jacques

Published
2021
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4. Early efficacy evaluation of mesenchymal stromal cells (MSC) combined to biomaterials to treat long bone non-unions

Author

Gómez-Barrena, Enrique, Padilla-Eguiluz, Norma, Rosset, Philippe, Gebhard, Florian, Hernigou, Philippe, Baldini, Nicola, Rouard, Helène, Sensebé, Luc, Gonzalo-Daganzo, Rosa-María, Giordano, Rosaria, García-Rey, Eduardo, Cordero-Ampuero, José, Rubio-Suárez, Juan Carlos, García-Simón, Marta Dominguez, Stanovici, Julien, Ehrnthaller, Christian, Huber-Lang, Markus, Flouzat-Lachaniette, Charles Henri, Chevallier, Nathalie, Donati, Davide Maria, Spazzoli, Benedetta, Ciapetti, Gabriela, Fleury, Sandrine, Fernandez, Manuel-Nicolás, Cabrera, José-Rafael, Avendaño-Solá, Cristina, Montemurro, Tiziana, Panaitescu, Carmen, Veronesi, Elena, Rojewski, Markus Thomas, Lotfi, Ramin, Dominici, Massimo, Schrezenmeier, Hubert, and Layrolle, Pierre

Published
2020
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5. Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for Controlled Ex-vivo Expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells

Author

Menneteau, Thomas, Fabre, Bertrand, Garrigues, Luc, Stella, Alexandre, Zivkovic, Dusan, Roux-Dalvai, Florence, Mouton-Barbosa, Emmanuelle, Beau, Mathilde, Renoud, Marie-Laure, Amalric, François, Sensébé, Luc, Gonzalez-de-Peredo, Anne, Ader, Isabelle, Burlet-Schiltz, Odile, and Bousquet, Marie-Pierre

Published
2019
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7. Feasibility and safety of treating non-unions in tibia, femur and humerus with autologous, expanded, bone marrow-derived mesenchymal stromal cells associated with biphasic calcium phosphate biomaterials in a multicentric, non-comparative trial

Author

Gómez-Barrena, Enrique, Rosset, Philippe, Gebhard, Florian, Hernigou, Philippe, Baldini, Nicola, Rouard, Helène, Sensebé, Luc, Gonzalo-Daganzo, Rosa M., Giordano, Rosaria, Padilla-Eguiluz, Norma, García-Rey, Eduardo, Cordero-Ampuero, José, Rubio-Suárez, Juan Carlos, Stanovici, Julien, Ehrnthaller, Christian, Huber-Lang, Markus, Flouzat-Lachaniette, Charles Henri, Chevallier, Nathalie, Donati, Davide Maria, Ciapetti, Gabriela, Fleury, Sandrine, Fernandez, Manuel-Nicolás, Cabrera, José-Rafael, Avendaño-Solá, Cristina, Montemurro, Tiziana, Panaitescu, Carmen, Veronesi, Elena, Rojewski, Markus Thomas, Lotfi, Ramin, Dominici, Massimo, Schrezenmeier, Hubert, and Layrolle, Pierre

Published
2019
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12. International Society for Cellular Therapy perspective on immune functional assays for mesenchymal stromal cells as potency release criterion for advanced phase clinical trials

Author

Galipeau, Jacques, Krampera, Mauro, Barrett, John, Dazzi, Francesco, Deans, Robert J., DeBruijn, Joost, Dominici, Massimo, Fibbe, Willem E., Gee, Adrian P., Gimble, Jeffery M., Hematti, Peiman, Koh, Mickey B.C., LeBlanc, Katarina, Martin, Ivan, McNiece, Ian K., Mendicino, Michael, Oh, Steve, Ortiz, Luis, Phinney, Donald G., Planat, Valerie, Shi, Yufang, Stroncek, David F., Viswanathan, Sowmya, Weiss, Daniel J., and Sensebe, Luc

Published
2016
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14. Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles

Author

Prel, Anne, Caval, Vincent, Gayon, Régis, Ravassard, Philippe, Duthoit, Christine, Payen, Emmanuel, Maouche-Chretien, Leila, Creneguy, Alison, Nguyen, Tuan Huy, Martin, Nicolas, Piver, Eric, Sevrain, Raphaël, Lamouroux, Lucille, Leboulch, Philippe, Deschaseaux, Frédéric, Bouillé, Pascale, Sensébé, Luc, and Pagès, Jean-Christophe

Published
2015
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17. Risk of tumorigenicity in mesenchymal stromal cell–based therapies—Bridging scientific observations and regulatory viewpoints

Author

Barkholt, Lisbeth, Flory, Egbert, Jekerle, Veronika, Lucas-Samuel, Sophie, Ahnert, Peter, Bisset, Louise, Büscher, Dirk, Fibbe, Willem, Foussat, Arnaud, Kwa, Marcel, Lantz, Olivier, Mačiulaitis, Romaldas, Palomäki, Tiina, Schneider, Christian K., Sensebé, Luc, Tachdjian, Gérard, Tarte, Karin, Tosca, Lucie, and Salmikangas, Paula

Published
2013
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21. Platelet lysate from whole blood-derived pooled platelet concentrates and apheresis-derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components

Author

Fekete, Natalie, Gadelorge, Mélanie, Fürst, Daniel, Maurer, Caroline, Dausend, Julia, Fleury-Cappellesso, Sandrine, Mailänder, Volker, Lotfi, Ramin, Ignatius, Anita, Sensebé, Luc, Bourin, Philippe, Schrezenmeier, Hubert, and Rojewski, Markus Thomas

Published
2012
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31. Culture and Use of Mesenchymal Stromal Cells in Phase I and II Clinical Trials

Author

Bourin Philippe, Sensebé Luc, Planat-Bénard Valérie, Roncalli Jérôme, Bura-Rivière Alessandra, and Casteilla Louis

Subjects
Internal medicine, RC31-1245
Abstract

Present in numerous tissues, mesenchymal stem cells/multipotent stromal cells (MSCs) can differentiate into different cell types from a mesoderm origin. Their potential has been extended to pluripotency, by their possibility of differentiating into tissues and cells of nonmesodermic origin. Through the release of cytokines, growth factors and biologically active molecules, MSCs exert important paracrine effects during tissue repair and inflammation. Moreover, MSCs have immunosuppressive properties related to non-HLA restricted immunosuppressive capacities. All these features lead to an increasing range of possible applications of MSCs, from treating immunological diseases to tissue and organ repair, that should be tested in phase I and II clinical trials. The most widely used MSCs are cultured from bone marrow or adipose tissue. For clinical trial implementation, BM MSCs and ADSCs should be produced according to Good Manufacturing Practices. Safety remains the major concern and must be ensured during culture and validated with relevant controls. We describe some applications of MSCs in clinical trials.

Published
2010
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32. Cell immaturity and white/beige adipocyte potential of primary human adipose-derived stromal cells are restrained by culture-medium TGFβ1

Author

Leménager, Hélène, primary, Fiévet, Loïc M. A., additional, Guilloton, Fabien, additional, Naji, Abderrahim, additional, Descamps, Jean-Gérard, additional, Chaput, Benoît, additional, Suganuma, Narufumi, additional, Pagès, Jean-Christophe, additional, Sensebé, Luc, additional, Carrière, Audrey, additional, Casteilla, Louis, additional, and Deschaseaux, Frédéric, additional

Published
2020
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34. Integrated transcriptomic, phenotypic, and functional study reveals tissue-specific immune properties of mesenchymal stromal cells

Author

Ménard, Cédric, primary, Dulong, Joëlle, additional, Roulois, David, additional, Hébraud, Benjamin, additional, Verdière, Léa, additional, Pangault, Céline, additional, Sibut, Vonick, additional, Bezier, Isabelle, additional, Bescher, Nadège, additional, Monvoisin, Céline, additional, Gadelorge, Mélanie, additional, Bertheuil, Nicolas, additional, Flécher, Erwan, additional, Casteilla, Louis, additional, Collas, Philippe, additional, Sensebé, Luc, additional, Bourin, Philippe, additional, Espagnolle, Nicolas, additional, and Tarte, Karin, additional

Published
2019
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35. Transportation Conditions for Prompt Use of Ex-vivo Expanded and Freshly Harvested Clinical Grade Bone Marrow MSC For Bone Regeneration

Author

Veronesi, Elena, Murgia, Alba, Caselli, Anna, Grisendi, Giulia, Piccinno, Maria Serena, Rasini, Valeria, Giordano, Rosaria, Montemurro, Tiziana, Bourin, Philippe, Sensebé, Luc, Rojewski, Markus T., Schrezenmeier, Hubert, Layrolle, Pierre, Ginebra, Maria Pau, Panaitescu, Carmen Bunu, Gómez-Barrena, Enrique, Catani, Fabio, Paolucci, Paolo, Burns, Jorge S., and Dominici, Massimo

Published
2014
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36. Osteoblastic differentiation and potent osteogenicity of three-dimensional hBMSC-BCP particle constructs

Author

Cordonnier, Thomas, Langonné, Alain, Corre, Pierre, Renaud, Audrey, Sensebé, Luc, Rosset, Philippe, Layrolle, Pierre, Sohier, Jérôme, Jehan, Frederic, Regenerating Bone defects using New biomedical Engineering approaches - REBORNE - - EC:FP7:HEALTH2010-01-01 - 2015-06-30 - 241879 - VALID, Physiopathologie des Adaptations Nutritionnelles (PhAN), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Laboratoire d'hématopoïèse [Tours], Université Francois Rabelais [Tours], Etablissement Français du Sang Centre-Atlantique (EA3855), EFS, Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Département de Stomatologie et chirurgie maxillo-faciale [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Etablissement français du sang [Pyrénées-Méditerranée] (EFS Pyrénées-Méditerranée), Départements de chirurgie orthopédique [CHRU Tours], This work was supported by the French National Research Agency (ATOS project) and partially funded by the Directorate-General for Research of the European Commission (Grant No. 241879) through the REBORNE project., European Project: 241879,EC:FP7:HEALTH,FP7-HEALTH-2009-single-stage,REBORNE(2010), Micro et Nanomédecines Biomimétiques, Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Theraptosis Research Laboratory, Theraptosis S.A., Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), STROMALab, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Centre National de la Recherche Scientifique (CNRS), Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes] (INSERM U1238), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Equipe de recherche clinique en Odontologie (ERT1051), Université de Nantes (UN), Micro et Nanomédecines Biomimétiques (MINT), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)-Université Francois Rabelais [Tours], and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université Francois Rabelais [Tours]

Subjects
Male, three dimensional constructs, biphasic calcium phosphate, Mice, Nude, Real-Time Polymerase Chain Reaction, Mice, Implants, Experimental, Osteogenesis, Animals, Humans, RNA, Messenger, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, bone tissue engineering, ComputingMilieux_MISCELLANEOUS, Aged, Cell Proliferation, Aged, 80 and over, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Osteoblasts, Tissue Scaffolds, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, glass beads, Cell Differentiation, Mesenchymal Stem Cells, Middle Aged, Microspheres, osteogenicity in nude mice, human bone marrow stromal cells, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Female, Hydroxyapatites, osteoblastic differentiation
Abstract

International audience; Bone tissue engineering usually consists of associating osteoprogenitor cells and macroporous scaffolds. This study investigated the in vitro osteoblastic differentiation and resulting in vivo bone formation induced by a different approach that uses particles as substrate for human bone marrow stromal cells (hBMSCs), in order to provide cells with a higher degree of freedom and allow them to synthesize a three-dimensional (3D) environment. Biphasic calcium phosphate (BCP) particles (35 mg, ~175 µm in diameter) were therefore associated with 4 X 10[5] hBMSCs. To discriminate the roles of BCP properties and cell-synthesized 3D environments, inert glass beads (GBs) of similar size were used under the same conditions. In both cases, high cell proliferation and extensive extracellular matrix (ECM) production resulted in the rapid formation of thick cell-synthesized 3D constructs. In vitro, spontaneous osteoblastic differentiation was observed in the 3D constructs at the mRNA and protein levels by monitoring the expression of Runx2, BMP2, ColI, BSP and OCN. The hBMSC–BCP particle constructs implanted in the subcutis of nude mice induced abundant ectopic bone formation after 8 weeks (~35%, n = 5/5). In comparison, only fibrous tissue without bone was observed in the implanted hBMSC–GB constructs (n = 0/5). Furthermore, little bone formation (~3%, n = 5/5) was found in hBMSC–macroporous BCP discs (diameter 8 X 3 mm). This study underlines the lack of correspondence between bone formation and in vitro differentiation assays. Furthermore, these results highlight the importance of using BCP as well as a 3D environment for achieving high bone yield of interest for bone engineering.

Published
2014
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37. Comparative study of the osteogenic ability of four different ceramic constructs in an ectopic large animal model

Author

Viateau, Véronique, MANASSERO, Mathieu, Sensebé, Luc, Langonné, Alain, Marchat, David, Logeart-Avramoglou, Delphine, Petite, Hervé, Bensidhoum, Morad, École nationale vétérinaire d'Alfort (ENVA), STROMALab, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Centre National de la Recherche Scientifique (CNRS), Etablissement Français du Sang Centre-Atlantique (EA3855), EFS, Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Bioingénierie et Bioimagerie Ostéo-articulaires, Biomécanique et Biomatériaux Ostéo-Articulaires (B2OA (UMR_7052)), École nationale vétérinaire d'Alfort (ENVA)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Etablissement Français du Sang-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016
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38. Inferior In Vivo Osteogenesis and Superior Angiogeneis of Human Adipose-Derived Stem Cells Compared with Bone Marrow-Derived Stem Cells Cultured in Xeno-Free Conditions

Author

Brennan, Meadhbh A., primary, Renaud, Audrey, additional, Guilloton, Fabien, additional, Mebarki, Miryam, additional, Trichet, Valerie, additional, Sensebé, Luc, additional, Deschaseaux, Frederic, additional, Chevallier, Nathalie, additional, and Layrolle, Pierre, additional

Published
2017
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40. Brief Report: Proteasomal Indoleamine 2,3-Dioxygenase Degradation Reduces the Immunosuppressive Potential of Clinical Grade-Mesenchymal Stromal Cells Undergoing Replicative Senescence

Author

Loisel, Séverine, primary, Dulong, Joëlle, additional, Ménard, Cédric, additional, Renoud, Marie-Laure, additional, Meziere, Nadine, additional, Isabelle, Bezier, additional, Latour, Maëlle, additional, Bescher, Nadège, additional, Pedeux, Rémy, additional, Bertheuil, Nicolas, additional, Flecher, Erwan, additional, Sensebé, Luc, additional, and Tarte, Karin, additional

Published
2017
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41. Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Author

Chabot, Valérie, Dromard, Cécile, Rico, Angélique, Langonné, Alain, Gaillard, Julien, Guilloton, Fabien, Casteilla, Louis, and Sensebé, Luc

Subjects
Platelet-Derived Growth Factor, Cell Movement, Research, Integrin beta1, Humans, Mesenchymal Stem Cells, Vitronectin, Cells, Cultured, Collagen Type I, Fibronectins, Receptors, Urokinase Plasminogen Activator, Signal Transduction
Abstract

Introduction Mesenchymal stem cells (MSC) are well described for their role in tissue regeneration following injury. Migratory properties of endogenous or administrated MSC are critical for tissue repair processes. Platelet-derived growth factor (PDGF) is a chemotactic growth factor that elicits mesenchymal cell migration. However, it is yet to be elucidated if signaling pathways other than direct activation of PDGF receptor (PDGF-R) are involved in PDGF-induced cell migration. Methods Knocking down and co-immunoprecipitation approaches were used to evaluate urokinase-type plasminogen activator receptor (uPAR) requirement and its interactions with proteins involved in migration mechanisms, in human MSC induced to migrate under PDGF-AB effect. Results We demonstrated that uPAR activation and its association with β1-integrin are required for PDGF-AB-induced migration. This phenomenon takes place in MSC derived from bone marrow and from adipose tissue. Conclusions We showed that PDGF-AB downstream signaling requires other effector molecules in MSC such as the uPA/uPAR system and β1 integrin signaling pathway known for their role in migration. These findings provide new insights in molecular mechanisms of PDGF-AB-induced migration of human MSC that may be relevant to control MSC function and tissue remodeling after injury. Electronic supplementary material The online version of this article (doi:10.1186/s13287-015-0163-5) contains supplementary material, which is available to authorized users.

Published
2015

42. Additional file 3: Figure S2. of Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Author

Chabot, Valérie, Dromard, Cécile, Rico, Angélique, Langonné, Alain, Gaillard, Julien, Guilloton, Fabien, Casteilla, Louis, and Sensebé, Luc

Subjects
body regions, nervous system, fungi
Abstract

Showing PDGF-AB enhances BM-MSC migration but not proliferation. (PDF 130 kb)

Published
2015
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43. Additional file 8: Figure S7. of Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Author

Chabot, Valérie, Dromard, Cécile, Rico, Angélique, Langonné, Alain, Gaillard, Julien, Guilloton, Fabien, Casteilla, Louis, and Sensebé, Luc

Subjects
body regions, nervous system, fungi, neoplasms, biological factors
Abstract

Showing PDGF enhanced uPAR/β1-integrin subunit interactions in migrating BM-MSC. (PDF 299 kb)

Published
2015
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44. Additional file 4: Figure S3. of Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Author

Chabot, Valérie, Dromard, Cécile, Rico, Angélique, Langonné, Alain, Gaillard, Julien, Guilloton, Fabien, Casteilla, Louis, and Sensebé, Luc

Subjects
body regions, nervous system, fungi, neoplasms, biological factors
Abstract

Showing regulation of uPAR and uPA expression in BM-MSC by inflammatory cytokines. (PDF 54 kb)

Published
2015
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49. Additional file 5: Figure S4. of Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Author

Chabot, Valérie, Dromard, Cécile, Rico, Angélique, Langonné, Alain, Gaillard, Julien, Guilloton, Fabien, Casteilla, Louis, and Sensebé, Luc

Subjects
body regions, nervous system, fungi, biological phenomena, cell phenomena, and immunity, biological factors
Abstract

Showing uPA/uPAR mediate PDGF-AB-induced BM-MSC migration on fibronectin and vitronectin. (PDF 45 kb)

Published
2015
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50. Additional file 7: Figure S6. of Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Author

Chabot, Valérie, Dromard, Cécile, Rico, Angélique, Langonné, Alain, Gaillard, Julien, Guilloton, Fabien, Casteilla, Louis, and Sensebé, Luc

Subjects
body regions, nervous system, fungi, neoplasms, biological factors
Abstract

Showing PDGF-AB effects on cell morphology and uPAR distribution. (PDF 51 kb)

Published
2015
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