7 results on '"SEGMENTED-FLOW"'
Search Results
2. Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples
- Author
-
Jialan Cao, Felix Richter, Michael Kastl, Jonny Erdmann, Christian Burgold, David Dittrich, Steffen Schneider, J. Michael Köhler, and G. Alexander Groß
- Subjects
dose–response ,droplet-based ,segmented-flow ,photo-fluorimetric ,flow sensor ,dynamic cultivation ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
Droplet-based microfluidics is a versatile tool to reveal the dose–response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose–response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose–response data for 1D and 2D dose–response screenings are presented.
- Published
- 2020
- Full Text
- View/download PDF
3. Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow.
- Author
-
Willrodt, Christian, Halan, Babu, Karthaus, Lisa, Rehdorf, Jessica, Julsing, Mattijs K., Buehler, Katja, and Schmid, Andreas
- Abstract
ABSTRACT The efficiency of biocatalytic reactions involving industrially interesting reactants is often constrained by toxification of the applied biocatalyst. Here, we evaluated the combination of biologically and technologically inspired strategies to overcome toxicity-related issues during the multistep oxyfunctionalization of ( R)-(+)-limonene to ( R)-(+)-perillic acid. Pseudomonas putida GS1 catalyzing selective limonene oxidation via the p-cymene degradation pathway and recombinant Pseudomonas taiwanensis VLB120 were evaluated for continuous perillic acid production. A tubular segmented-flow biofilm reactor was used in order to relieve oxygen limitations and to enable membrane mediated substrate supply as well as efficient in situ product removal. Both P. putida GS1 and P. taiwanensis VLB120 developed a catalytic biofilm in this system. The productivity of wild-type P. putida GS1 encoding the enzymes for limonene bioconversion was highly dependent on the carbon source and reached 34 g L
tube −1 day−1 when glycerol was supplied. More than 10-fold lower productivities were reached irrespective of the applied carbon source when the recombinant P. taiwanensis VLB120 harboring p-cymene monooxygenase and p-cumic alcohol dehydrogenase was used as biocatalyst. The technical applicability for preparative perillic acid synthesis in the applied system was verified by purification of perillic acid from the outlet stream using an anion exchanger resin. This concept enabled the multistep production of perillic acid and which might be transferred to other reactions involving volatile reactants and toxic end-products. Biotechnol. Bioeng. 2017;114: 281-290. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
4. Segmented continuous-flow multiplex polymerase chain reaction microfluidics for high-throughput and rapid foodborne pathogen detection.
- Author
-
Shu, Bowen, Zhang, Chunsun, and Xing, Da
- Subjects
- *
FOOD pathogens , *DIAGNOSTIC microbiology , *PATHOGENIC microorganisms , *DNA analysis , *POLYMERASE chain reaction , *MICROFLUIDICS , *ANALYTICAL chemistry - Abstract
Highlights: [•] A high-throughput and rapid microfluidic method for pathogen detection is proposed. [•] The method offers a simple and convenient way toward high-throughput DNA analysis. [•] Important parameters of segmented continuous-flow multiplex PCR were investigated. [•] The proposed method is suitable for high-throughput biomedical monitoring. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
5. Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples
- Author
-
Cao, Richter, Kastl, Erdmann, Burgold, Dittrich, Schneider, Köhler, and Groß
- Subjects
microbial ,droplet-based ,photo-fluorimetric ,flow sensor ,screening ,proliferation ,droplet generator ,bacterial ,dose–response ,dynamic cultivation ,segmented-flow - Abstract
Droplet-based microfluidics is a versatile tool to reveal the dose&ndash, response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose&ndash, response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose&ndash, response data for 1D and 2D dose&ndash, response screenings are presented.
- Published
- 2020
- Full Text
- View/download PDF
6. Infrared thermo-spectroscopic imaging of styrene radical polymerization in microfluidics
- Author
-
Meguya Ryu, Christophe Pradere, T. Sato, Ryuichi Nakatani, James A. Kimber, Sergei G. Kazarian, Teruaki Hayakawa, A.A. Hovhannisyan, M. Romano, Junko Morikawa, Department of Materials Science and Engineering, Nagoya Institute of Technology, Institut de Mécanique et d'Ingénierie de Bordeaux (I2M), École Nationale Supérieure d'Arts et Métiers (ENSAM), Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Institut Polytechnique de Bordeaux-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-École Nationale Supérieure d'Arts et Métiers (ENSAM), and HESAM Université (HESAM)-HESAM Université (HESAM)-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Technology ,Engineering, Chemical ,Materials science ,Infrared ,General Chemical Engineering ,Microfluidics ,Radical polymerization ,Analytical chemistry ,FABRICATION ,0904 Chemical Engineering ,02 engineering and technology ,01 natural sciences ,Chemical reaction ,Industrial and Manufacturing Engineering ,0905 Civil Engineering ,Styrene ,Polymerization ,chemistry.chemical_compound ,[SPI]Engineering Sciences [physics] ,Engineering ,Environmental Chemistry ,WATER ,Microscale thermal imaging ,Infrared spectroscopy ,TEMPERATURE ,Microscale chemistry ,KINETICS ,ComputingMilieux_MISCELLANEOUS ,Science & Technology ,010401 analytical chemistry ,SEGMENTED-FLOW ,Engineering, Environmental ,General Chemistry ,Chemical Engineering ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,IR spectroscopic imaging ,Chemical species ,0907 Environmental Engineering ,chemistry ,CHIP CALORIMETRY ,0210 nano-technology ,MICRODEVICES - Abstract
A novel infrared (IR) thermo-spectroscopic imaging technique is applied to the simultaneous measurements of IR transmittance spectroscopic images and thermal emission images of heat and mass transfers of styrene monomer polymerization in microfluidics. The heat released is observed in the mixing layer between two laminar flows of styrene monomer and toluene containing an initiator for polymerization. The concentration of chemical species and the independently measured temperature distribution within the mixing layer are determined at the microscale. This is the first report, of such a non-invasive method, to determine both the chemical composition and temperature distribution in microfluidic chemical reactions.
- Published
- 2017
7. Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow
- Author
-
Willrodt, Christian, Halan, Babu, Karthaus, L., Rehdorf, J., Julsing, M.K., Buehler, Katja, Schmid, Andreas, Willrodt, Christian, Halan, Babu, Karthaus, L., Rehdorf, J., Julsing, M.K., Buehler, Katja, and Schmid, Andreas
- Abstract
The efficiency of biocatalytic reactions involving industrially interesting reactants is often constrained by toxification of the applied biocatalyst. Here, we evaluated the combination of biologically and technologically inspired strategies to overcome toxicity-related issues during the multistep oxyfunctionalization of (R)-(+)-limonene to (R)-(+)-perillic acid. Pseudomonas putida GS1 catalyzing selective limonene oxidation via the p-cymene degradation pathway and recombinant Pseudomonas taiwanensis VLB120 were evaluated for continuous perillic acid production. A tubular segmented-flow biofilm reactor was used in order to relieve oxygen limitations and to enable membrane mediated substrate supply as well as efficient in situ product removal. Both P. putida GS1 and P. taiwanensis VLB120 developed a catalytic biofilm in this system. The productivity of wild-type P. putida GS1 encoding the enzymes for limonene bioconversion was highly dependent on the carbon source and reached 34 g Ltube−1 day−1 when glycerol was supplied. More than 10-fold lower productivities were reached irrespective of the applied carbon source when the recombinant P. taiwanensis VLB120 harboring p-cymene monooxygenase and p-cumic alcohol dehydrogenase was used as biocatalyst. The technical applicability for preparative perillic acid synthesis in the applied system was verified by purification of perillic acid from the outlet stream using an anion exchanger resin. This concept enabled the multistep production of perillic acid and which might be transferred to other reactions involving volatile reactants and toxic end-products.
- Published
- 2016
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.