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When one walks toward a crowd of pedestrians, dealing with their biological motion while controlling one's own self-motion is a difficult perceptual task. Limb articulation of a walker is naturally coupled to the walker's translation through the scene and allows the separation of optic flow generated by self-motion from the biological motion of other pedestrians. Recent research has shown that if limb articulation and translation mismatch, such as for walking in place, self-motion perception becomes biased. This bias may reflect an illusory motion attributed to the pedestrian crowd from the articulation of their limbs. To investigate this hypothesis, we presented observers with a simulation of forward self-motion toward a laterally moving crowd of point-light walkers and asked them to report the perceived lateral speed of the crowd. To investigate the dependence of the crowd speed percept on biological motion, we also included conditions in which the points of the walker were spatially scrambled to destroy body form and limb articulation. We observed illusory crowd speed percepts that were related to the articulation rate of the biological motion. Scrambled walkers also produced illusory motion but it was not related to articulation rate. We conclude that limb articulation induces percepts of crowd motion that can be used for interpreting self-motion toward crowds., Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2024.)

Ciliates are microbial eukaryotes with distinct somatic and germline genomes. Postzygotic development involves extensive remodeling of the germline genome to form somatic chromosomes. Ciliates therefore offer a valuable model for studying the architecture and evolution of programed genome rearrangements. Current studies usually focus on a few model species, where rearrangement features are annotated by aligning reference germline and somatic genomes. Although many high-quality somatic genomes have been assembled, a high-quality germline genome assembly is difficult to obtain due to its smaller DNA content and abundance of repetitive sequences. To overcome these hurdles, we propose a new pipeline, SIGAR (S plit-read I nference of G enome A rchitecture and R earrangements) to infer germline genome architecture and rearrangement features without a germline genome assembly, requiring only short DNA sequencing reads. As a proof of principle, 93% of rearrangement junctions identified by SIGAR in the ciliate Oxytricha trifallax were validated by the existing germline assembly. We then applied SIGAR to six diverse ciliate species without germline genome assemblies, including Ichthyophthirius multifilii , a fish pathogen. Despite the high level of somatic DNA contamination in each sample, SIGAR successfully inferred rearrangement junctions, short eliminated sequences, and potential scrambled genes in each species. This pipeline enables pilot surveys or exploration of DNA rearrangements in species with limited DNA material access, thereby providing new insights into the evolution of chromosome rearrangements. [ABSTRACT FROM AUTHOR]

Since the size of the image file is not reduced after encryption, the flow rate of image file transmission cannot be reduced. Transmission time is wasted. To meet the requirements of security and real-time, wavelet transform and chaotic image encryption technology are combined. A cross-chaos map has been proposed based on Devaney's theory. The image pixels are scrambled under the control of high-dimensional chaotic sequence, which is generated by the cross chaotic map. The image pixels are substituted by the ciphertext feedback algorithm. The algorithm can relate to encryption parameters with plaintext, and can make one plaintext byte affect more ciphertext bytes. In the process of image wavelet transform, image encryption and image compression are combined. The low frequency region of the wavelet transform is encrypted. Security analysis and experimental validation illuminated that the algorithm has high security, good encryption speed, and compression effect. The algorithm has good practical value. [ABSTRACT FROM AUTHOR]

Abstract: In plants important questions relate to the mechanisms that control signaling between the histogenic cell layers of apical meristems and developing organs. The Arabidopsis putative atypical leucine-rich repeat receptor-like kinase STRUBBELIG (SUB) regulates amongst others floral organ shape, the plane of cell division in cells of the first subepidermal cell layer of floral meristems, ovule integument morphogenesis, and root hair patterning. Reporter assays using a functional translational fusion between SUB and EGFP indicate that SUB expression is largely confined to interior tissues in young flowers, ovules, and roots. In contrast, SUB mRNA expression can be monitored in all cell layers of those tissues. Specifically, SUB protein is not detectable in cells that show a sub mutant phenotype. Rather, SUB is detected in directly neighbouring cells in flower and ovule primordia, or in cells that are separated from mutant cells by two cell diameters in the root. Inhibitor studies corroborate a posttranscriptional regulation of SUB. Phenotypic analysis of sub-1 plants expressing a SUB:EGFP gene under the control of tissue and epidermis-specific promoters support the notion that SUB-dependent signal transduction relies on the production of secondary intercellular signals. The combined results indicate that SUB acts in a non-cell-autonomous fashion, functions in a radial inside-out signaling process, and mediates cell morphogenesis and cell fate across clonally distinct cell layers in floral primordia, developing ovules, and root meristems. [Copyright &y& Elsevier]

Using functional magnetic resonance imaging (fMRI), we investigated the implicit language processing of kanji and kana words (i.e., hiragana transcriptions of normally written kanji words) and non-words. Twelve right-handed native Japanese speakers performed size judgments for character stimuli (implicit language task for linguistic stimuli), size judgments for scrambled-character stimuli (implicit language task for non-linguistic stimuli), and lexical decisions (explicit language task). The size judgments for scrambled-kanji stimuli and scrambled-kana stimuli produced activations on the bilateral lingual gyri (BA 18), the bilateral occipitotemporal regions (BA 19/37), and the bilateral superior and inferior parietal cortices (BA 7/40). Interestingly, besides these areas, activations of the left inferior frontal region (Broca''s area, BA 44/45) and the left posterior inferior temporal cortex (PITC, BA 37), which have been considered as language areas, were additionally activated during size judgment for kanji character stimuli. Size judgment for kana character stimuli also activated Broca''s area, the left PITC, and the left supramarginal gyrus (SMG, BA 40). The activations of these language areas were replicated in the lexical decisions for both kanji and kana. These findings suggest that language processing of both kanji and kana scripts is obligatory to literate Japanese subjects. Moreover, comparison between the scrambled kanji and the scrambled kana showed no activation in the language areas, while greater activation in the bilateral fusiform gyri (left-side predominant) was found in kanji vs. kana comparison during the size judgment and the lexical decision. Kana minus kanji activated the left SMG during the size judgment, and Broca''s area and the left middle/superior temporal junction during the lexical decision. These results probably reflect that in implicit or explicit reading of kanji words and kana words (i.e., hiragana transcriptions of kanji words), although using largely overlapping cortical regions, there are still some differences. Kanji reading may involve more heavily visual orthographic retrieval and lexical–semantic system through the ventral route, while kana transcriptions of kanji words require phonological recoding to gain semantic access through the dorsal route. [Copyright &y& Elsevier]

There is currently much interest in understanding the mechanisms of normal and aberrant lung alveolarization, particularly in the context of bronchopulmonary dysplasia, a common complication of preterm birth where alveolarization is impeded. To this end, the parenteral administration of pharmacological agents that modulate biochemical pathways, or facilitate modulation of gene expression in transgenic animals, has facilitated the discovery and validation of mechanisms that direct lung development. Such studies include control interventions, where the solvent vehicle, perhaps containing an inactive form of the agent applied, is administered; thereby providing a well-controlled point of reference for the analysis of the partner experiment. In the present study, the impact of several widely used control interventions in developing C57Bl/6J mouse pups was examined for effects on lung structure and the lung transcriptome. Parenteral administration of scrambled microRNA inhibitors (called antagomiRs) that are used to control in vivo microRNA neutralization studies, impacted lung volume, septal thickness, and the transcriptome of developing mouse lungs; with some effects dependent upon nucleotide sequence. Repeated intraperitoneal isotonic saline injections altered lung volume, with limited impact on the transcriptome. Parenteral administration of the tamoxifen solvent Miglyol accelerated mouse pup growth, and changed the abundance of 73 mRNA transcripts in the lung. Tamoxifen applied in Miglyol-in the absence of Cre recombinase-decreased pup growth, lung volume, and lung alveolarization and changed the abundance of 298 mRNA transcripts in the lung. These data demonstrate that widely used control interventions can directly impact lung alveolarization and the lung transcriptome in studies on lung development. Anat Rec, 302:346-363, 2019. © 2018 Wiley Periodicals, Inc., (© 2018 Wiley Periodicals, Inc.)

The Arabidopsis root epidermal cells decide their fates (root-hair cell and non-hair cell) according to their position. SCRAMBLED (SCM), an atypical leucine-rich repeat receptor-like kinase (LRR RLK) mediates the positional information to the epidermal cells enabling them to adopt the proper fate. Via feedback regulation, the SCM protein accumulates preferentially in cells adopting the root-hair cell fate. In this study, we determine that TRY, but not the related factor CPC, is responsible for this preferential SCM accumulation. We observed severe reduction of SCM::GUS expression in the try-82 mutant root, but not in the cpc-1 mutant. Furthermore, the overexpression of TRY by CaMV35S promoter caused an increase in the expression of SCM::GUS in the root epidermis. Intriguingly, the overexpression of CPC by CaMV35S promoter repressed the expression of SCM::GUS. Together, these results suggest that TRY plays a unique role in generating the appropriate spatial expression of SCM.

Prostate cancer (CaP) is the second leading malignancy in men. The role of epithelial cell adhesion molecule (EpCAM), also known as CD326, in CaP progression and therapeutic resistance is still uncertain. Here, we aimed to investigate the roles of EpCAM in CaP metastasis and chemo/radioresistance. Expression of EpCAM in CaP cell lines and human CaP tissues was assessed using immunofluorescence and immunohistochemistry, respectively. EpCAM was knocked down (KD) in PC-3, DU145 and LNCaP-C4-2B cells using small interfering RNA (siRNA), and KD results were confirmed by confocal microscope, Western blotting and quantitative real time polymerase chain reaction (qRT-PCR). Cell growth was evaluated by proliferation and colony formation assays. The invasive potential was assessed using a matrigel chamber assay. Tumorigenesis potential was measured by a sphere formation assay. Chemo-/radiosensitivity were measured using a colony formation assay. Over-expression of EpCAM was found in primary CaP tissues and lymph node metastases including cancer cells and surrounding stromal cells. KD of EpCAM suppressed CaP proliferation and invasive ability, reduced sphere formation, enhanced chemo-/radiosensitivity, and down-regulated E-cadherin, p-Akt, p-mTOR, p-4EBP1 and p-S6K expression in CaP cells. Our findings suggest that EpCAM plays an important role in CaP proliferation, invasion, metastasis and chemo-/radioresistance associated with the activation of the PI3K/Akt/mTOR signaling pathway and is a novel therapeutic target to sensitize CaP cells to chemo-/radiotherapy., (Copyright © 2013 Elsevier Ltd. All rights reserved.)