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42 results on '"SALERNO, Giuseppina"'

13. A serum fucose-binding lectin (D1FBL) from adult Didentrarchus labrax is expressed in larva and juvenile tissue and contained in eggs

Author

PARISI, Maria Giovanna, CAMMARATA, Matteo, BENENATI, Gigliola, SALERNO, Giuseppina, MANGANO, Valentina, VIZZINI, Aiti, PARRINELLO, Nicolo', Parisi, MG, Cammarata, M, Benenati, G, Salerno, G, Mangano, V, Vizzini, A, and Parrinello, N

Subjects
Settore BIO/05 - Zoologia, Dicentrarchus labrax
Abstract

The purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of Dicentrarchus labrax (DlFBL) have been previously reported. We now describe the distribution and expression of DlFBL during fish ontogeny. Immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days posthatching larvae to juveniles). Another fucose-binding lectin, similar to DlFBL in biochemical, immunochemical and agglutinating properties, was extracted and purified from eggs and appeared to be localized in the embryo yolk sack residual. DlFBL was found in columnar and goblet cells of the intestinal epithelium of larvae (from 20 days post-hatching) and juveniles and in parenchymal tissue of juveniles. DlFBL mRNA and protein were detected in the intestinal epithelium and in hepatocytes. An amplification product from degenerate primers indicates that lectin isotypes with DlFBL epitopes are expressed in eggs and embryos. Whether the lectin fraction isolated from eggs and embryos includes DlFBL of maternal origin remains unclear.

Published
2010

14. The Ciona intestinalis prophenoloxidase activating system during LPS inflammatory reaction

Author

CAMMARATA, Matteo, MANGANO, Valentina, ARIZZA, Vincenzo, CIANCIOLO COSENTINO, Chiara, PARRINELLO, Daniela, VAZZANA, Mirella, VIZZINI, Aiti, SALERNO, Giuseppina, PARRINELLO, Nicolo', Cammarata, M, Mangano, V, Arizza, V, Cianciolo Cosentino, C, Parrinello, D, Vazzana, M, Vizzini, A, Salerno, G, and Parrinello, N

Subjects
Ciona intestinalis, LPS
Published
2009

15. An Attempt to re-examine the immune role of Ciona intestinalis hemocytes

Author

PARRINELLO, Nicolo', ARIZZA, Vincenzo, CAMMARATA, Matteo, VIZZINI, Aiti, PARRINELLO, Daniela, VAZZANA, Mirella, GIARAMITA, Francesca Tiziana, SALERNO, Giuseppina, PERGOLIZZI, Margherita, SANFRATELLO, Maria Antonietta, Parrinello, N, Arizza, V, Cammarata, M, Vizzini, A, Parrinello, D, Vazzana, M, Giaramita, FT, Salerno, G, Pergolizzi, M, and Sanfratello, MA

Subjects
Ciona intestinalis, hemocytes
Published
2009

16. Enhanced CiTNFα expression is an inflammatory response of the ascidian Ciona intestinalis

Author

PARRINELLO, Nicolo', VIZZINI, Aiti, SALERNO, Giuseppina, GIARAMITA, Francesca Tiziana, PARRINELLO, Daniela, SANFRATELLO, Maria Antonietta, MANSUETO, Valentina, VAZZANA, Mirella, ARIZZA, Vincenzo, CAMMARATA, Matteo, Parrinello, N, Vizzini, A, Salerno, G, Giaramita, FT, Parrinello, D, Sanfratello, MA, Mansueto, V, Vazzana, M, Arizza, V, and Cammarata, M

Subjects
CiTNFα, inflammatory response, Ciona intestinalis
Published
2009

18. Effects of cadmium on expression of the hsp70 in sea bass (Dicentrarchus labrax L., Osteichthyes. Moronidae) blood cells

Author

CELI, Monica, VAZZANA, Mirella, SALERNO, Giuseppina, DI BELLA, Maria Luigia, ARIZZA, Vincenzo, PARRINELLO, Nicolo', Celi, M, Vazzana, M, Salerno, G, Di Bella, ML, Arizza, V, and Parrinello, N

Subjects
Stress protein, Settore BIO/05 - Zoologia, Fish blood cell, Cadmium
Abstract

To gain further insight into the role of cortisol in Wsh innate immune responses, we cloned and sequenced a 2592 bp cDNA from sea bass (Dicentrarchus labrax) peritoneal leukocytes (PCLs) encoding a glucocorticoid receptor (DlGR1). The deduced aminoacid sequence displayed that DlGR1 belong to a multigenic family of steroid hormone receptors, and exhibited high homology (80%) to the Burton’s mouth breeder (Haplochromis burtoni) HbGR1. The DlGR1 functional domains presented homologies with those of several vertebrate species. In situ hybridization assay revealed that DlGR1 was expressed in macrophages and neutrophils from the peritoneal cavity. Since in a previous paper, sea bass PCL chemiluminescence response (CL) has been related to increased respiratory burst of phagocytes stimulated with zymosan, PCLs, preincubated in vitro with cortisol at various concentrations, were assayed for their CL response. Dose-dependent cortisol inhibitory eVects, and signiWcant competitive activity of a low concentration of mifepristone (RU486), a glucocorticoid-receptor blocker, supported that cortisol–GR interaction was involved in modulating CL response via a genomic pathway. Results also indicated that cortisol could be eVective through an additional not-genomic way, and showed that high doses of RU486 exerted an inhibitory eVect on PCL chemiluminescence activity.

Published
2008

19. Tunicate immunocytes can be cytotoxic toward foreign cells

Author

ARIZZA, Vincenzo, GIARAMITA, Francesca Tiziana, PARRINELLO, Daniela, VAZZANA, Mirella, VIZZINI, Aiti, SALERNO, Giuseppina, CAMMARATA, Matteo, PARRINELLO, Nicolo', ARIZZA, V, GIARAMITA, FT, PARRINELLO, D, VAZZANA M, VIZZINI, A, SALERNO, G, CAMMARATA, M, and PARRINELLO, N

Subjects
Settore BIO/05 - Zoologia, Tunicate, cytotoxic toward
Published
2008

22. Expression of a glucocorticoid receptor (D1GR1) in several tissues of the teleost fish Dicentrarchus labrax

Author

VAZZANA, Mirella, VIZZINI, Aiti, SALERNO, Giuseppina, DI BELLA M. L, CELI, Monica, PARRINELLO, Nicolo', VAZZANA M, VIZZINI A, SALERNO G, DI BELLA M L, CELI M, and PARRINELLO N

Subjects
Stre, Dicentrarchus labrax, Glucocorticoid receptor, Glucocorticoid receptors, In situ hybridization, Immunohistochemistry, Stress
Abstract

Since glucocorticoids have a role in maintaining the homeostatic status in fish, in the present paper mRNA expression (in situ hybridization) and tissue immunohistochemical localization of a glucocorticoid receptor (DlGR1) in several Dicentrarchus labrax organs are reported. Riboprobe and specific antibodies were prepared by using the DlGR1 that has been previously cloned and sequenced from peritoneal cavity leukocytes. Both mRNA and receptor were identified in head kidney, spleen, gills, intestine, heart and liver tissues. The functional roles of DlGR1 localization are discussed.

Published
2008

25. Comparative analysis of fucose binding lectins isolated and characterized from different teleost species, and distribution of a F-Lectin during Dicentrarchus labrax ontogenesis

Author

CAMMARATA, Matteo, PARISI, Maria Giovanna, BENENATI, Gigliola, MANGANO, Valentina, VIZZINI, Aiti, SALERNO, Giuseppina, PARRINELLO, Daniela, VAZZANA, Mirella, PARRINELLO, Nicolo', CAMMARATA M, PARISI MG, BENENATI G, MANGANO V, VIZZINI A, SALERNO G, PARRINELLO D, VAZZANA M, and PARRINELLO N

Subjects
teleost, lectin, Dicentrarchus labrax, Fucose
Published
2008

33. Serum lectins in fish innate immunity: molecular and functional aspects

Author

CAMMARATA, Matteo, BENENATI, Gigliola, PARISI, Maria Giovanna, PARRINELLO, Daniela, SALERNO, Giuseppina, VAZZANA, Mirella, VIZZINI, Aiti, PARRINELLO, Nicolo', Vasta, G, CAMMARATA M, G BENENATI, G PARISI, D PARRINELLO, G SALERNO, M VAZZANA, A VIZZINI, GR VASTA, and N PARRINELLO

Subjects
Fucose-binding lectins, Dicentrarchus labrax, Sparus aurata
Abstract

Fucose-binding lectins (FBL) are present in tissues and fluids from invertebrates and vertebrates. The lectin repertoires in teleost fish are highly diversified and recently has been described the structure of the fucose-binding agglutinin that revealed a novel lectin fold (the “F-type” eel (Anguilla anguilla) fold), which shared a unique fucose-binding sequence motif contained both in carbohydrate-binding proteins and unrelated proteins. In this report, we describe serum FBL from sea bass Dicentrarchus labrax and sea bream Sparus aurata. These lectins were purified, characterized, cloned and sequenced. Studies on structural aspects, biological activity, tissue distribution as well as ontogenetic aspects were carried out. In addition, results on inflammatory response and opsonic activity against bacteria suggested that D. labrax FBL is involved in innate immunity. Finally a new galactose binding lectin with agglutinating activity against bacteria purified from Dicentrarchus labrax serum was also described and compared with FBL.

Published
2007

35. Localizzazione di una lectina di 'tipo F' nell’adulto e nell’ontogenesi di Dicentrarchus labrax

Author

PARISI, Maria Giovanna, CAMMARATA, Matteo, SALERNO, Giuseppina, BENENATI, Gigliola, VIZZINI, Aiti, VAZZANA, Mirella, PARRINELLO, Nicolo', PARRINELLO, Daniela, PARINELLO D, PARISI MG, CAMMARATA M, SALERNO G, BENENATI G, VIZZINI A, VAZZANA M, PARINELLO D, PARRINELLO N, and Parrinello, D.

Abstract

The purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of Dicentrarchus labrax (DlFBL) have been previously reported. We now describe the distribution and expression of DlFBL during fish ontogeny. Immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days posthatching larvae to juveniles). Another fucose-binding lectin, similar to DlFBL in biochemical, immunochemical and agglutinating properties, was extracted and purified from eggs and appeared to be localized in the embryo yolk sack residual. DlFBL was found in columnar and goblet cells of the intestinal epithelium of larvae (from 20 days post-hatching) and juveniles and in parenchymal tissue of juveniles. DlFBL mRNA and protein were detected in the intestinal epithelium and in hepatocytes. An amplification product from degenerate primers indicates that lectin isotypes with DlFBL epitopes are expressed in eggs and embryos. Whether the lectin fraction isolated from eggs and embryos includes DlFBL of maternal origin remains unclear.

Published
2007

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