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Lymphocytes characterized by in vitro rosette formation with sheep erythrocytes were enumerated in blood of normal and measles virus-vaccinated monkeys. Rosetting lymphocyte counts increased significantly as a result of vaccination. Concomitantly, HAI-antibody, undetectable before vaccination, became detectable, suggesting that the simple and inexpensive technique of enumerating rosetting lymphocytes can be used as an alternative to other more cumbersome and expensive methods for evaluation of development of cell-mediated immunity following measles virus vaccination.

Sera of 173 patients with various forms of liver disease along with serum precipitates produced by polyethylene glycol were screened for the presence of a microsomal antigen referred to as ubiquitous tissue antigen (UTA) and its antibody by double diffusion precipitation in agarose gel. UTA was detected in 7 of 26 patients with chronic active hepatitis, 1 of 5 with alcoholic hepatitis, 2 of 14 with alcoholic cirrhosis and 18 of 98 with hepatoma. Antibodies to UTA were found only in 2 patients with chronic active hepatitis, 1 with alcoholic cirrhosis and 1 with hepatoma. No UTA or its antibody were noted in sera of 5 patients with alcoholic fatty liver, 10 patients with hepatitis B, and 15 asymptomatic carriers of HBsAg. Positivity for the UTA or its antibody was restricted to severe, chronic cases irrespective of diagnosis, indicating that persistent tissue destruction might be necessary for antigen release or antibody formation.