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The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand- and fingerprints, clothing, car interiors, computer accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology.

Often in the examination of bloodstained fingermarks discussion occurs around whether to prioritise the fingerprint evidence or focus on the biological evidence. Collecting a sample for genetic profiling may result in the loss of ridge detail that could have been used for fingerprint comparison. Fingermark enhancement and recovery methods along with sample collection methods could also compromise downstream genetic analysis. Previous forensic casework has highlighted circumstances where, after enhancement had been performed, it would have been extremely valuable to both identify the body fluid and generate a DNA profile from the same sample. We enhanced depletion series of fingermarks made in blood, using single treatments consisting of aqueous amido black, methanol-based amido black, acid yellow and leucocrystal violet, and exposure to long wave UV light. We then extracted the DNA and RNA for profiling, to assess the recovery and detection of genetic material from the enhanced fingermarks. We have shown that genetic profiling of bloodstained fingermarks can be successful after chemical enhancement; however it may still be necessary to prioritise evidence types in certain circumstances. From our results it appears that even with visible bloodstained fingermarks, leucocrystal violet can reduce the effectiveness of subsequent messenger RNA profiling. Aqueous amido black and acid yellow also have adverse effects on messenger RNA profiling of depleted fingermarks with low levels of cellular material. These results help with forensic decision-making by expanding knowledge of the extent of the detrimental effects of blood-enhancement reagents on both DNA profiling and body fluid identification using messenger RNA profiling.

Cannabis sativa is both an illegal drug and a legitimate crop. The differentiation of illegal drug Cannabis from non-drug forms of Cannabis is relevant in the context of the growth of fibre and seed oil varieties of Cannabis for commercial purposes. This differentiation is currently determined based on the levels of tetrahydrocannabinol (THC) in adult plants. DNA based methods have the potential to assay Cannabis material unsuitable for analysis using conventional means including seeds, pollen and severely degraded material. The purpose of this research was to develop a single nucleotide polymorphism (SNP) assay for the differentiation of ''drug'' and ''non-drug'' Cannabis plants. An assay was developed based on four polymorphisms within a 399 bp fragment of the tetrahydrocannabinolic acid (THCA) synthase gene, utilising the snapshot multiplex kit. This SNP assay was tested on 94 Cannabis plants, which included 10 blind samples, and was able to differentiate between ''drug'' and ''non-drug'' Cannabis in all cases, while also differentiating between Cannabis and other species. Non-drug plants were found to be homozygous at the four sites assayed while drug Cannabis plants were either homozygous or heterozygous.

In this paper, we discuss the DNA results obtained from the analysis of cellular material located beneath the fingernails of two women in separate cases of homicide. In both cases, the deceased females had been submerged in water, the first in bath water and the second in seawater, for 2 and 3 h, respectively. Mixed DNA profiles were obtained in each case indicating the presence of foreign DNA in these samples. These results indicate the valuable forensic evidence that can be obtained from samples that may be considered unlikely to provide DNA results and the high priority that should be placed on the routine DNA analysis of material from deceased's fingernails, regardless of the scenes that they are found in, including submersion in water.

Use of the product rule which implies the assumption of within and between locus independence, is still common, particularly in the United States of America. Whilst it may be considered by some to be an acceptable approximation it is not logical to suggest that independence testing somehow "validates" its use. This paper discusses the nature of this fallacy.

The New Zealand DNA Databank was established following the introduction of legislation in August 1996. Using the Second Generation Multiplex (SGM), DNA profiles from over 13,000 convicted offenders and volunteer donors have been completed to the National DNA Database. Since June 1998, DNA profiles from over 1,400 unsolved crimes have been entered onto the Crime Sample Database. Of all unsolved crimes analysed, 33% are linked to individuals and 21% are linked to other unsolved crimes. Several high profile types of case including homicides, sexual offenses and burglaries are amongst those regularly solved.

This paper extends the calculation of conditional probabilities from those given by Balding and Nichols to casework situations where a series of possible DNA types are possible. Such situations may occur when a sample is identified containing a mixture of DNA from two or more people or where extra information can be determined about the subpopulation under consideration by analysis of additional samples. Using this approach, the error in the estimated likelihood ratios is expected to reduce as the number of additional individuals typed from the subpopulation increases.

The DNA analysis technique tRFLP (terminal restriction fragment length polymorphism) was utilised to develop an alternative to the traditional microscopic examination of pollen in order to facilitate the use of this valuable forensic evidence type. The gene chosen for this study was the alcohol dehyrogenase 1 gene. Statistical analysis established that the tRFLP technique was a reliable and reproducible technique that could provide considerable discriminating power between both plant and pollen species. This indicates that the tRFLP could be a suitable technique for the analysis of pollen communities as forensic evidence.

The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were used for the identification of vaginal secretion: Vag triplex (MYOZ1, CYP2B7P1 and MUC4) and a Lactobacillus-specific Lacto triplex (Ljen, Lcris, Lgas). The laboratories used different chemistries and instrumentation and all were able to successfully isolate and detect mRNA in dried stains. The simultaneous extraction of RNA and DNA allowed for positive identification of the tissue/fluid source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling, also from old and compromised casework samples. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid identification method that can easily be combined with current STR typing technology.

Over the past fifty years, radiation protection legislation in the UK has developed from a narrow industry-specific base to a comprehensive package of regulations and supporting Approved Code of Practice, with additional provisions for nuclear installations. Development of this legislation mirrors progress in international understanding about the risks from exposure to ionising radiation. The current Ionising Radiations Regulations 1985 largely implement the Euratom 1980 Basic Safety Standards Directive and place particular emphasis on the need to keep exposures as low as reasonably practicable. The regulations have been underpinned by the development of the concept of the Tolerability of Risk and the application of the ALARP/ALARA principle, particularly at nuclear installations. Analysis of dose data on HSE's Central Index of Dose Information has shown the general success of this approach in the UK; the data have also allowed targeting of inspection effort. Currently, the Health and Safety Commission and Executive are developing plans for implementing the revised EU Basic Safety Standards Directive.

While most potentially hazardous industrial undertakings have safety objectives of some kind, the nuclear power industry is relatively unique in the extent to which it has attempted to refine and quantify its particular ones. This paper discusses the reasons for this and reviews the range and variety of nuclear safety objectives by reference to studies carried out within the European Community and the International Atomic Energy Agency. It refers to recent developments in the UK which illustrate the advantages and disadvantages of attempting to use quantitative safety objectives in the public arena and concludes with some thoughts about the likely future development of nuclear safety objectives.

Allele frequencies for the 10 STR loci included in the AMP Fl STR ® SGM Plus™ multiplex system have been determined for the four major sub-populations of New Zealand.

A coherent method is offered to estimate likelihood ratios Es wird eine verstandliche Methode zur Abschatzung der for DNA match probabilities from mixed racial populations Likelihood Ratios von DNA Match Wahrscheinlichkeiten in that avoids the approach of reporting separate estimates for rassisch gernischten Populationen vorgestellt, rnit der es each race. The method is demonstrated for some cases vermieden wird, separate Abschatzungen fur jede einzelne involving profiles derived from several individuals and Rasse vornehmen zu miissen. Die Methode wird an einigen incorporates a correction for 'subpopulation' effects. Falle demonstriert, die von verschiedenen Individuen abgeleitete Profile beinhalten. Eine Korrektur fur Subpopulationseffekte findet Beriicksichtigung.

The polarization in p-3He elastic scattering at 30 MeV in the angular range 33° to 160° c.m. has been measured. The magnitude of the polarization is very much greater than that predicted from an optical-model analysis of cross section data, but is consistent with the values found at lower energies.

States of 9 B and 8 B were studied by measuring differential cross sections for the reactions (p, d) and (p, t) from a 10 B target at E p = 49.5 MeV. Strongly excited states were observed at 0.0, 2.33 and 11.66 MeV in 9 B and at 0.0 and 2.29 MeV in 8 B. The angular distribution of elastic scattering of 49.5 MeV protons from 10 B was also measured and fitted using an optical-model calculation. The distributions of (p, d) and (p, t) cross sections were compared with DWBA predictions and a spin assignment was made for the 2.29 MeV state in 8 B.

The energy spectra of deuterons, tritons and 3 He particles from the reactions 3 He(p, d)2p, 4 He(p, t)2p and 4 He(p, 3 He)pn have been measured at angles between 6° and 60° lab. The 3 He(p, d)2p reaction was studied at both 30.5 and 49.5 MeV incident proton energies, while the other two reactions were studied at 49.5 MeV only. The energy spectra are compared with calculations based on the Watson-Migdal model of final-state interactions.

The angular distribution of the reaction 4 He(p, d) 3 He at 49.5 MeV has been measured over the c.m. angular range of 11°–165°. This angular distribution, together with the angular distributions of the reactions 4 He(p, t)2p, 4 He(p, 3 He)pn, 3 He(p, d)2p at 49.5 MeV and 3 He(p, d)2p at 30.5 MeV reported previously, have been analysed in terms of the plane wave Born approximation, DWBA and diffraction theories.

Differential cross-section and polarization measurements on the p- 3 He system at 30 and 50 MeV have been analysed in terms of real phase shifts. It is shown that there is not sufficient data at either energy to define a unique solution.

Cross sections have been measured for the transitions to the ground state and first excited state of 8Be and to the two 2+ levels at 16.63 MeV and 16.93 MeV excitation in the reaction 10B(p, 3He) at Ep = 49.5 MeV. The angular distributions of the cross section were compared with DWBA predictions.

No evidence has been found for the excitation of states of 3 He through the reaction 3 He(p, p′) 3 He ∗ with 30 MeV protons.