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There are some errors in edition descriptions of Ma Peizhi's《医略存真》《马氏医论》均为马培之著作，二书版本的著录现有书目皆存讹误，对二书关系的相关研究也显不足。《医略存真》版本系统貌似简单，实际存在早期刊刻和后期增订、补刻的区别。《马氏医论》于民国绍兴医药学报上连载，以此为基础，相关整理著作不断问世，然其刊本、抄本之间增删情况复杂。《医略存真》《马氏医论》在体例上有一致性，内容上有颇多相同之处，但差异亦为明显。诸多证据表明：《马氏医论》当为《医略存真》成书来源之一，而探讨二者之间的关系，可为进一步分析《医略存真》成书过程及马培之学术思想的变化提供依据。.

Antimony(III) nitrilotriacetate complexes with mixed Nta3− and HNta2− ligands of composition (NH4)2Sb(Nta)(HNta) · nH2O (I) and Na2Sb(Nta)(HNta) · 2H2O (II) were synthesized. The proposed structures were confirmed by chemical analysis, x-ray diffraction, IR spectroscopy, and thermogravimetry. The cytotoxic properties of antimony chelates in vitro and their antitumor activity with respect to Ehrlich adenocarcinoma (EAC) in mice were studied. Compounds I and II produced a significant (60 – 90%) increase in the survival rate of test mice with ascitic EAC, at an optimum therapeutic dose of 25 – 50 mg/kg, in the absence of significant toxicity in this dose range. The results show good prospects in the search for new antitumor agents among Sb(III) complexes with aminopolycarboxy ligands.

Based on Muskhelishvili's integral equation and the Schwarz-Christoffel integral, a numerical procedure is developed for solving the stress fields on plates with emphasis on the region around crack tips. The difficulties in the numerical determination of accessory parameters in the Schwarz-Christoffel transformation are overcome by separating the improper integral into two parts: a finite form of integral in a continuous region which can be integrated exactly; and, a definite integral free of singularity which can easily be computed numerically. Furthermore, the difficulties in the numerical computation of the kernel function in the boundary integral equation are resolved by finding the limit of the kernel function as the source point approaches the field point. Some numerical results of plane-stress problems are provided to demonstrate the accuracy of this approach.

To investigate the elastic compressive behavior of gusset plate connections, eight tests are performed with gusset plate thickness, gusset plate size, and out‐of‐plane restraint of the diagonal bracing member as test parameters. The effects of the framing members on the gusset plate are neglected in the testing program. It is found that the primary failure mode of gusset plate connections loaded in compression is gusset plate buckling. The buckling can be classified as sway buckling and local buckling depending on the out‐of‐plane restraint of the diagonal bracing member. The elastic buckling strength of the specimens is affected significantly by the gusset plate thickness and the out‐of‐plane restraint of the bracing member. The finite‐element analysis by the program ANSYS gives reasonable predictions of the buckling strength of the gusset plate connection. A parametric study was performed using ANSYS. It is found from the parametric study that splicing member should always be extended toward the beam an...

catena-Poly[bis(3-methyl-4-nitropyridine N-oxide-O)cadmium-di-μ-bromo], [Cd(C 6 H 6 N 2 O 3 ) 2 Br 2 ], which belongs to a group of potential nonlinear optical materials, crystallizes in the non-centrosymmetric space group Fdd2. The Cd atom is octahedrally coordinated to two O atoms from the trans 3-methyl-4-nitropyridine N-oxide ligands in the axial positions and four Br atoms lying in the equatorial plane. The coordination octahedra form one-dimensional chains along the short c axis by edge-sharing through the Br atoms, with each Cd atom located on a crystallographic diad axis

We present an end-pumped continuous-wave Raman laser at 1180 nm with a 60 mm long pure crystal SrWO4 and an Nd:GdAl3(BO3)4 crystal. The highest output power Raman laser at 1180 nm is 15.5 mW corresponding to the pump power of 1.75 W. Results indicate that the intense self-doubling frequency effect of GdAl3(BO3)4 reduces the 1180 nm Raman laser output.

Polymeric carboxylethylgermanium sesquioxide has an infinite sheet structure in which the basic unit of the network is a 12-membered ring made up of six Ge tetrahedra bridged by 0 atoms. The carboxylate chains are arranged alternately above and below the Ge-O network around the ring. Sheets are vertically linked by hydrogen-bond pairs between the carboxylate groups

[HgBr 2 (C 6 H 6 N 2 O 3 )] cristallise dans P2 1 /c avec a=13.338, b=11.599, c=7.096A, β=93.74°, Z=4; affinement jusqu'a R=0.061. La structure forme un polymere grâce a six atomes pontants. L'atome Hg est coordine a deux atomes O de la pyridine et quatre atomes Br. L'atome Hg possede une coordination octaedrique deformee.

A new triterpenoid saponin named clinopodiside was isolated from Clinopodum Chinense (Benth) O. Kuntze. The structure of clinopodiside A was elucidated by spectroscopic and X-ray diffraction analysis as 3-O-beta-D-glucopyranosyl (1----6)-[beta-D-glucopyranosyl (1----4)]-beta-D-glucopyranosyl-olean-11, 13 (18)-diene-3 beta, 16 beta, 23, 28-tetrol.

The Se atom in N-[α-(N-succimidyl)benzyl]selenamorpholine {alternative name: 1-[phenyl(1,4-selenazan-4-yl)methyl]pyrrolidine-2,5-dione}, C15H18N2O2Se, is two-coordinate in a bent C2Se skeleton [Se—C = 1.946 (4) and 1.948 (5) A; C—Se—C = 92.9 (2) A].

A very highly purified fraction of KB cell DNA polymerase-alpha, prepared with a monoclonal antibody, contains DNA primase activity. The primase synthesizes oligonucleotide chains initiated with ATP in a reaction that is resistant to alpha-amanitin and strictly dependent on added template and ribonucleoside triphosphates (rNTPs). In the presence of added dNTPs and M13 DNA template, the primase produces a uniform population of oligoribonucleotides, predominantly hexamers to decamers, that are extended by polymerase-alpha into DNA chains up to 3000 nucleotides long. There is no evidence for nucleotide preferences at RNA/DNA junctions. In the absence of added dNTPs, the oligomeric products are heterogeneous in size and composition and susceptible to cleavage by pancreatic DNase I due to their content of short oligodeoxynucleotide tracts synthesized by primase from trace contaminant dNTPs in the rNTP substrates. The primase and polymerase-alpha activities are distinguishable by several physical and chemical criteria, and the primase reaction is only partially sensitive to two potent, independent monoclonal antibodies that neutralize polymerase-alpha. Although the presence of both primase and polymerase-alpha activities in a highly purified immune complex prepared with a monoclonal antibody argues for their tight physical association, the chemical, physical, and immunological discriminations indicate the two catalytic entities are functionally and structurally distinct.

We have successfully established 16 stable murine hybridomas that secrete monoclonal antibodies specific for human DNA polymerase alpha. The results of immunocytochemical studies, using 4 of these monoclonal antibodies and immunoperoxidase detection methods, document the exclusively intranuclear localization of DNA polymerase alpha in three separate lines of cultured human cells. By light microscopy, the immunoperoxidase reaction product exhibits a diffuse pattern of distribution within the nucleoplasm, but nucleoli are clearly negative. In cultures of the transformed lines, KB nd BeWo, more than 955 of the cells are positive, suggesting that intranuclear DNA polymerase alpha antigens persist throughout the mitotic cycle. In striking contrast, in the normal diploid fibroblast line, WI-38, a smaller fraction of the cultured cells is positive, and there is no detectable polymerase alpha antigen in the closely apposed cells of microcolonies that are presumed to be contact-arrested and no longer mitotically cycling. In cells in mitosis that have dissolved their nuclear envelopes (and are thus transiently anucleate), the anti-polymerase alpha reaction continues to be strongly positive, and in this single circumstance the reaction product is diffusely distributed throughout the cell cytoplasm. Initial electron microscopic examination of KB cells confirms and extends these observations. The immunoperoxidase reaction product is essentially limited to the nuclear compartment and is predominantly distributed in the midzonal region of the nucleoplasm between centrally disposed nucleoli and peripherally located blocks of condensed chromatin.

We report the successful establishment of 16 stable murine hybridoma monoclones that produce homogeneous antibodies against KB cell DNA polymerase alpha. All of the antibodies exhibit specific binding of polymerase alpha activity, and 3 of them possess anti-polymerase alpha neutralizing activity. None of the antibodies interacts detectably with KB cell DNA polymerases beta or gamma. All of the 5 antibodies so far examined demonstrate linear Scatchard binding plots and very high binding affinities, with equilibrium dissociation constants (Kd) ranging between 3.2 x 10(-9) and 3.4 x 10(-10) M. These monoclonal antibodies comprise a set of powerful and specific reagents that should facilitate the development and application of novel approaches to the complex biochemical mechanisms of mammalian DNA replication.

The oligonucleotide primers synthesized by a highly purified KB fraction containing DNA primase and DNA polymerase-alpha activities display reproducible alterations of structure and composition in response to dNTPs. These observations are sufficiently explained by a novel model of primase catalysis that defines the primase as a complex enzyme with distinct catalytic properties that are regulated by mechanisms exquisitely sensitive to dNTP concentration. The enzyme performs the template-directed, de novo synthesis of 1 unit of oligoribonucleotide (canonical priming reaction) and then elongates that moiety to a limited extent by several cycles of addition of short tracts of homogeneous oligoribonucleotide or oligodeoxynucleotide. The oligomeric units have modal lengths of approximately 11- to 14- nucleotides that are postulated to reflect the inherent processivity of the catalytic mechanisms. Elongation is accomplished by two catalytic centers, or conformers of a single center, that are synchronously coupled, mutually exclusive, and extremely stringent for respective rNTP and dNTP substrates. Transitions between these two catalytic modes are regulated by dNTPs and demonstrable at dNTP:rNTP concentrations of less than or equal to 10(-4). In the absence of dNTPs, the primase produces a family of oligoribonucleotides, approximately 24- to 36- nucleotides long; at dNTP levels between 0.08 and 0.80 micron, the primase synthesizes mixed oligomers composed of strictly alternating tandem arrays of oligoribo- and oligodeoxynucleotide units; and at dNTP levels greater than or equal to 4.8 micron, the primase becomes stabilized in the deoxy mode after the initial oligoribo leads to oligodeoxy transition and products contain only a single 5' -terminal unit of oligoribonucleotide. The model predicts that the physiologically significant primer for DNA polymerase-alpha is a mixed 5' -oligoribo-3' -oligodeoxynucleotide and the signal which governs the switch from RNA leads to DNA synthesis is intrinsic in the primase mechanism and is generated by ambient dNTPs.

We report the successful establishment of 16 stable murine hybridoma monoclones that produce homogeneous antibodies against KB cell DNA polymerase alpha. All of the antibodies exhibit specific binding of polymerase alpha activity, and 3 of them possess anti-polymerase alpha neutralizing activity. None of the antibodies interacts detectably with KB cell DNA polymerases beta or gamma. All of the 5 antibodies so far examined demonstrate linear Scatchard binding plots and very high binding affinities, with equilibrium dissociation constants (Kd) ranging between 3.2 x 10(-9) and 3.4 x 10(-10) M. These monoclonal antibodies comprise a set of powerful and specific reagents that should facilitate the development and application of novel approaches to the complex biochemical mechanisms of mammalian DNA replication.

We have successfully established 16 stable murine hybridomas that secrete monoclonal antibodies specific for human DNA polymerase alpha. The results of immunocytochemical studies, using 4 of these monoclonal antibodies and immunoperoxidase detection methods, document the exclusively intranuclear localization of DNA polymerase alpha in three separate lines of cultured human cells. By light microscopy, the immunoperoxidase reaction product exhibits a diffuse pattern of distribution within the nucleoplasm, but nucleoli are clearly negative. In cultures of the transformed lines, KB nd BeWo, more than 955 of the cells are positive, suggesting that intranuclear DNA polymerase alpha antigens persist throughout the mitotic cycle. In striking contrast, in the normal diploid fibroblast line, WI-38, a smaller fraction of the cultured cells is positive, and there is no detectable polymerase alpha antigen in the closely apposed cells of microcolonies that are presumed to be contact-arrested and no longer mitotically cycling. In cells in mitosis that have dissolved their nuclear envelopes (and are thus transiently anucleate), the anti-polymerase alpha reaction continues to be strongly positive, and in this single circumstance the reaction product is diffusely distributed throughout the cell cytoplasm. Initial electron microscopic examination of KB cells confirms and extends these observations. The immunoperoxidase reaction product is essentially limited to the nuclear compartment and is predominantly distributed in the midzonal region of the nucleoplasm between centrally disposed nucleoli and peripherally located blocks of condensed chromatin.

A very highly purified fraction of KB cell DNA polymerase-alpha, prepared with a monoclonal antibody, contains DNA primase activity. The primase synthesizes oligonucleotide chains initiated with ATP in a reaction that is resistant to alpha-amanitin and strictly dependent on added template and ribonucleoside triphosphates (rNTPs). In the presence of added dNTPs and M13 DNA template, the primase produces a uniform population of oligoribonucleotides, predominantly hexamers to decamers, that are extended by polymerase-alpha into DNA chains up to 3000 nucleotides long. There is no evidence for nucleotide preferences at RNA/DNA junctions. In the absence of added dNTPs, the oligomeric products are heterogeneous in size and composition and susceptible to cleavage by pancreatic DNase I due to their content of short oligodeoxynucleotide tracts synthesized by primase from trace contaminant dNTPs in the rNTP substrates. The primase and polymerase-alpha activities are distinguishable by several physical and chemical criteria, and the primase reaction is only partially sensitive to two potent, independent monoclonal antibodies that neutralize polymerase-alpha. Although the presence of both primase and polymerase-alpha activities in a highly purified immune complex prepared with a monoclonal antibody argues for their tight physical association, the chemical, physical, and immunological discriminations indicate the two catalytic entities are functionally and structurally distinct.