401 results on '"S. Störkel"'
Search Results
2. Quantifying resection quality in radical prostatectomy through histopathologic scoring of positive surgical margins
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M.F. Möller, D. Gödde, R.F.M.S. Keller, and S. Störkel
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medicine.medical_specialty ,business.industry ,Prostatectomy ,Urology ,medicine.medical_treatment ,media_common.quotation_subject ,lcsh:Diseases of the genitourinary system. Urology ,lcsh:RC870-923 ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,Resection ,Medicine ,Quality (business) ,Radiology ,Positive Surgical Margin ,business ,media_common - Published
- 2020
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3. Günther Schubert
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S. Störkel
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media_common.quotation_subject ,Art history ,Art ,Pathology and Forensic Medicine ,media_common - Published
- 2018
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4. Calprotectin im Stuhl. Ein potentieller Biomarker für akute Appendizitis
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S Störkel, Peter C. Ambe, Daniel Gödde, Hubert Zirngibl, and S Jansen
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Gastroenterology - Published
- 2016
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5. Extranodale Metastasierung ist ein ungünstiger prognostischer Faktor bei Patienten mit Lymphknoten-positivem Darmkrebs
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S Störkel, Daniel Gödde, Peter C. Ambe, Hubert Zirngibl, and S Jansen
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Gastroenterology - Published
- 2016
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6. Nierenzelltumoren
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S. Störkel
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Pathology and Forensic Medicine - Abstract
Nierentumoren wurden in den letzten Jahren zunehmend genauer klassifiziert und haben neue Untergruppen erhalten, die aufgrund der differenten Entwicklungswege unterschiedliche Prognosen haben und spezifische Therapien erfordern. Zur immunhistologischen Differenzierung steht eine Reihe von Antikorpern zur Verfugung. Mit der Feststellung eines Gendefekts – Mutation, Deletion, Trisomien und Monosomien, Aktivierung von Onkogenen usw. – kann eine zielgerichtete Therapie u. a. mit Antikorpern oder Tyrosinkinaseinhibitoren in die Signalwege eingreifen. Die Nierentumordiagnostik durch den Pathologen nimmt dabei eine besonders wichtige Stellung ein.
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- 2010
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7. Das Amyloid der Langerhansschen Inseln und seine Beziehung zum Diabetes mellitus* 1
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H.-M. Schneider, S. Störkel, and W. Will
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Pathology ,medicine.medical_specialty ,medicine.anatomical_structure ,business.industry ,Amyloidosis ,Diabetes mellitus ,Medicine ,General Medicine ,business ,medicine.disease ,Grading (tumors) ,B cell - Abstract
After grading into 4 groups of severity the extent of amyloidosis of the islets of Langerhans was investigated in 60 patients with diabetes and in 60 without. Distinction of 3 stages of diabetes according to clinical parameters showed clear connections between the stage of diabetes and the extent of amyloidosis. Development of amyloid locally may be evidence for progressive B cell insufficiency in diabetes of adults.
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- 2008
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8. Epidemiologische und klinische Aspekte der fokalnodulären Hyperplasie der Leber: Eine Auswertung von 886 Fällen
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Manfred Thelen, Achim Heintz, S. Störkel, K F Kreitner, and Schild H
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medicine.medical_specialty ,Pathology ,business.industry ,Focal nodular hyperplasia ,Physiology ,Connective tissue ,General Medicine ,Hyperplasia ,medicine.disease ,medicine.anatomical_structure ,Family planning ,Liver tissue ,Pill ,Epidemiology ,medicine ,business ,Hormone - Abstract
The objective of this study was to check whether there is a link between focalnodular hepatic hyperplasia of the liver and use of birth control pills and if so what it is. Focalnodular hepatic hyperplasias consists of hepatocytes Kupffers cells proliferating hepatic ducts connective tissue and vessels. Characteristically on the cut surface of a focalnodular hyperplasia specimen is central star shaped scar tissue with radial branches. The nodular tissue is sharply delineated from the surrounding liver tissue without actually forming an anatomic capsule. Based on the literature and authors observations 886 cases were assembled for analysis. Results are as follows: Roughly 20% of tumors occur in children and adolescents barely 18% in males. Use of steroid hormones for contraceptive purposes was found in only 27.8% of patients. Tumor size does not correlate with length of use of birth control pills. In males and patients under 20 tumors are on the average larger than in women and patients over 19. On the other hand birth control pills are assumed to promote growth of an existing focalnodular hyperplasia for the following reason: Women using oral contraceptives on the average have larger tumors than non-users; oral contraceptive users are younger than the comparison group when the diagnosis is made; symptoms occur more frequently among pill user; however it is concluded that oral contraceptives can be precluded as a causal pathogenetic factor in focalnodular hyperplasia.
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- 2008
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9. Das metanephroide Adenom der Niere - eine neue Differentialdiagnose zum Nephroblastom und Nierenzellkarzinom im Erwachsenenalter
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C. Brinkschmidt and S. Störkel
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Gynecology ,medicine.medical_specialty ,Renal cell carcinoma ,business.industry ,Urology ,medicine ,Adult Nephroblastoma ,medicine.disease ,business - Published
- 2008
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10. Molekulargenetische Veränderungen in Nierenzellkarzinomen
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S. Störkel, Jens Atzpodien, and N. Buentig
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Gynecology ,medicine.medical_specialty ,business.industry ,Urology ,medicine ,business - Abstract
Im letzten Jahrzehnt wurden zahlreiche genetische Veranderungen identifiziert, die in die Pathogenese und Progression des Nierenzellkarzinoms involviert sind. Diese Erkenntnisse werden auf die Diagnose und Behandlung des Nierenzellkarzinoms einen wesentlichen Einfluss haben. Dieser Artikel gibt eine Ubersicht sowohl uber die allgemeinen Prinzipien der Tumorentwicklung als auch uber die spezifischen Mechanismen, die der Karzinogenese des Nierenzellkarzinoms zugrunde liegen.
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- 2002
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11. Genomic imbalances in 61 renal cancers from the proximal tubulus detected by comparative genomic hybridization
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W. Brenner, S. Störkel, Michael Mende, S. Naumann, J. Decker, Bernhard Zabel, and Dirk Reutzel
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Adult ,Male ,Lung Neoplasms ,Cell ,Bone Neoplasms ,Genomics ,Biology ,Kidney Tubules, Proximal ,Recurrence ,Genetics ,medicine ,Humans ,Molecular Biology ,Genetics (clinical) ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Chromosome Aberrations ,Genome, Human ,Nucleic Acid Hybridization ,Renal cancers ,Middle Aged ,Molecular biology ,Carcinoma, Papillary ,Kidney Neoplasms ,Survival Rate ,medicine.anatomical_structure ,Cancer research ,Female ,Neoplasm Recurrence, Local ,Adenocarcinoma, Clear Cell ,Comparative genomic hybridization - Abstract
Comparative genomic hybridization (CGH) has been applied to characterize 61 primary renal cell carcinomas derived histogenetically from the proximal tubulus. The tumor samples comprised 46 clear-cell renal cell carcinomas (ccRCCs) and 15 papillary renal cell carcinomas (pRCCs). Changes in the copy number of entire chromosomes or subregions were detected in 56 tumors (92%). In ccRCCs, losses of chromosome 3 or 3p (63%); 14q (30%); 9 (26%); 1 and 6 or 6q (17% each); 4 and 8 or 8p (15% each); 22 (11%); 2 or 2q and 19 (9% each); 7q, 10, 16, 17p, 18, and Y (7% each); and 5, 11, 13, 15, and 21 (4% each) were detected. Most frequent genomic gains in ccRCC were found on chromosome 5 (63%); 7 (35%); 1 or 1q (33%); 2q (24%); 8 or 8q, 12, and 20 (20% each); 3q (17%); 16 (15%); 19 (13%); 6 and 17 or 17q (11% each); and 4, 10, 11, 21, and Y (9% each). In pRCCs, gains in the copy number of chromosomes 7 and 17 (7/15, each) and 16 and 20 (6/15, each) were frequent. One pRCC showed amplification of subchromosome regions 2q22→q33, 16q, 17q and the entire X chromosome. In pRCC, losses were less frequently seen than gains. Losses of chromosomes 1, 14, 15, and Y (3/15 each) and 2, 4, 6, and 13 (2/15 each) were observed. In ccRCCs, statistical evaluation revealed significant correlations of chromosomal imbalances with tumor stage and grade, i.e., a gain in copy number of chromosome 5 correlated positively with low tumor grade, whereas a gain of chromosomes 10 and 17 correlated positively with high tumor grade. Furthermore, loss of chromosome 4 correlated positively with high tumor stage.
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- 2001
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12. Interleukin-7 or Interleukin-15 Enhances Survival ofMycobacterium tuberculosis-Infected Mice
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P. K. E. Trinder, Markus Maeurer, Wolfgang Walter, Kirsten Freitag, Gerhard Hommel, S. Störkel, and Derek Atkins
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Adoptive cell transfer ,medicine.medical_treatment ,Immunology ,Spleen ,Biology ,Microbiology ,Mice ,Immune system ,medicine ,Animals ,Tuberculosis ,Interleukin-15 ,Mice, Inbred BALB C ,Interleukin-7 ,Interleukin ,Mycobacterium tuberculosis ,T lymphocyte ,Adoptive Transfer ,Disease Models, Animal ,Infectious Diseases ,medicine.anatomical_structure ,Cytokine ,Interleukin 15 ,Microbial Immunity and Vaccines ,Cytokines ,Female ,Parasitology ,Tumor necrosis factor alpha - Abstract
Both antigen-presenting cells and immune effector cells are required to effectively eradicate or containMycobacterium tuberculosis-infected cells. A variety of cytokines are involved to ensure productive “cross talk” between macrophages and T lymphocytes. For instance, infection of macrophages with mycobacteria leads to effective interleukin-7 (IL-7) and IL-15 secretion, and both cytokines are able to maintain strong cellular immune responses of α/β and γ/δ T cells. Here we show that either cytokine is able to enhance survival ofM. tuberculosis-infected BALB/c mice significantly compared to application of IL-2, IL-4, or phosphate-buffered saline (as a control). Enhanced survival could be achieved only when IL-7 or IL-15 was delivered as a treatment (i.e., 3 weeks postinfection), not when it was administered at the time of infection. Increased survival ofM. tuberculosis-infected animals was observed following passive transfer of spleen cells harvested fromM. tuberculosis-infected, IL-7- or IL-15-treated animals, but not after transfer of spleen cells obtained from mice which received either cytokine alone. Histological examination revealed that IL-7 and IL-15 failed to significantly impact on the number and composition of granulomas formed or the bacterial load. Our data indicated that administration of IL-7 or IL-15 toM. tuberculosis-treated animals resulted in a qualitatively different cellular immune response in spleen cells as reflected by increased tumor necrosis factor alpha and decreased gamma interferon secretion in response toM. tuberculosis-infected antigen-presenting cells.
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- 2000
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13. Epithelial tumors of the kidney. Pathological subtyping and cytogenetic correlation
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S. Störkel
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Pathology ,medicine.medical_specialty ,Kidney ,Adenoma ,Urology ,Cell ,Chromophobe cell ,Biology ,medicine.disease ,Subtyping ,medicine.anatomical_structure ,medicine ,Carcinoma ,Pathological ,Clear cell - Abstract
The recent new morphological classification of epithelial renal tumors has overcome the former unspecified adenoma and carcinoma classification by introducing a specific subtyping. Today we divide in oncocytic and metanephrogenic renal cell adenomas, clear cell-, papillary-, chromophobe-, and collecting duct associated renal cell carcinomas, and transitional cell- and neuroendocrine renal carcinomas. These entities are characterized by a definite immunohistological marker spectrum and differing histogenetic pathways. New cytogenetic data have proven specific chromosomal aberrations for the different tumor types and have confirmed the morphological classification. Certain genetic changes are correlated to specific pathological phenotypes especially in oncocytic tumors. It is possible now to propose an in part hypothetical kidney tumor model which describes the development and progression of adenomas and carcinomas of the kidney in an adenoma-carcinoma sequence.
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- 1999
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14. Inverse regulation of vascular endothelial growth factor and VHL tumor suppressor gene in sporadic renal cell carcinomas is correlated with vascular growth: an in vivo study on 29 tumors
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Jürgen Brieger, C. Huber, H.J. Decker, S. Störkel, E.J. Weidt, and P Schirmacher
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Adult ,Male ,Vascular Endothelial Growth Factor A ,Pathology ,medicine.medical_specialty ,endocrine system diseases ,Tumor suppressor gene ,Angiogenesis ,Ubiquitin-Protein Ligases ,Endothelial Growth Factors ,Biology ,urologic and male genital diseases ,medicine.disease_cause ,Ligases ,chemistry.chemical_compound ,Drug Discovery ,medicine ,Humans ,Genes, Tumor Suppressor ,Carcinoma, Renal Cell ,Genetics (clinical) ,Aged ,Lymphokines ,Kidney ,Neovascularization, Pathologic ,Vascular Endothelial Growth Factors ,Tumor Suppressor Proteins ,Proteins ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,Gene Expression Regulation, Neoplastic ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,Clear cell renal cell carcinoma ,medicine.anatomical_structure ,chemistry ,Von Hippel-Lindau Tumor Suppressor Protein ,Molecular Medicine ,Female ,Carcinogenesis ,Clear cell - Abstract
Tumors associated with the VHL (von Hippel-Lindau) disease, such as hemangioblastomas and renal carcinomas and their sporadic counterparts, are cystic and well vascularized. Mutations of the VHL tumor-suppressor gene and elevated levels of vascular endothelial growth factor (VEGF) have been described in these tumors. The upregulation of VEGF has been shown in vitro as a consequence of alteration of the VHL gene. No comprehensive in vivo analysis has yet been carried out of the factors affecting tumor growth, vascularization, VEGF, and VHL expression. We performed immunohistochemistry and mRNA studies on primary sporadic renal carcinomas and matching normal renal tissue. We semiquantitatively analyzed 29 renal carcinomas (22 clear cell, 5 chromophilic, 2 chromophobic tumors) for VHL mRNA, and VEGF expression for morphology and tumor size. Immunohistochemistry was carried out for VEGF protein expression, vascularization, and macrophage infiltration. Vascularization of the chromophilic renal carcinomas was lower than that of the clear cell type of renal carcinoma. Low VEGF protein expression was seen in four of the five chromophilic renal carcinomas. We found two groups of clear cell renal cell carcinoma: one with reduced VHL mRNA and increased VEGF mRNA, and the other without significantly altered VHL or VEGF mRNAs. Tumor vascularization was correlated with VEGF protein and seemed to be independent of macrophage infiltration. Our in vivo findings support the inverse relationship between the regulation of VHL and that of VEGF. Our data also indicate that there may be an VHL-independent pathway for the induction of tumor vascularization.
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- 1999
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15. Das AFP-positive Nierenzellkarzinom
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Ulrich Witzsch, A. Kollias, S. Störkel, E. Becht, and V. Poulakis
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business.industry ,Renal cell carcinoma ,Urology ,Cancer research ,Medicine ,business ,medicine.disease - Published
- 1999
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16. Retroperitonealfibrose und Arthritis - Manifestation derselben Erkrankung
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H E Stierle, S Störkel, and A Thiele
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musculoskeletal diseases ,medicine.medical_specialty ,Pathology ,business.industry ,medicine.medical_treatment ,Rheumatoid nodule ,Arthritis ,Synovectomy ,medicine.disease ,Retroperitoneal fibrosis ,Rheumatology ,Surgery ,Fibrosis ,Internal medicine ,Rheumatoid arthritis ,medicine ,Rheumatoid factor ,medicine.symptom ,business - Abstract
Retroperitoneal fibrosis is a disorder in which the retroperitoneal fat is the site of a subacute and chronic inflammatory reaction and is subsequently replaced by dense fibrotic tissue. Rheumatoid nodules are chronic granulomata occurring at sites of pressure and movement, both near the body surface and internally. A 55-year-old sales-manager was admitted to radiation synovectomy after a 5 year history of excessive right and left knee effusions. There were no other clinical or laboratory abnormalities. The patient did not respond to either radioisotope synoviorthesis using radioactive Yttrium (90 Y), or to open synovectomy and prostetic surgery of the right knee. One year later, surgery of left ureter was necessary. Histological findings revealed the diagnosis of Ormond's disease. Comparative histological studies of synovial membrane of knee and retroperitoneal tissues showed local necrosis, fibrin deposition, lining cell proliferation, and infiltration by lymphocytes. Diagnosis of arthritis complicated by retroperitoneal rheumatoid nodules and retroperitoneal fibrosis was made. Serum rheumatoid factor has been negative. For the last 3 years, the patient has been on successful therapy with azathioprine. Rheumatoid nodules of the retroperitoneum have vanished completely and frequency of knee effusions decreased.
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- 1998
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17. Villöses Adenom des Nierenbeckens und Ureters
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K. Willenbrock, H. Frickmann, S. Jungblut, J. Bargon, S. Störkel, and P. Hanke
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Gynecology ,Villous adenoma ,medicine.medical_specialty ,medicine.anatomical_structure ,Ureter ,business.industry ,Urology ,medicine ,business ,medicine.disease ,Renal pelvis - Abstract
Villose Andenome des harnableitenden Systems sind extrem seltene Tumoren aus dem Formenkreis der adenoepithelialen Metaplasien, die mit anderen Neoplasien, insbesondere Karzinomen, vergesellschaftet sein konnen. Wir beschreiben den Fall einer 85 Jahre alten Patientin mit einem villosen Adenom des Nierenbeckens und Ureters.
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- 2006
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18. Choristom des Taschenbandes
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S. Störkel and H.-G. Kempf
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Gynecology ,medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,Head and neck surgery ,medicine ,business - Abstract
Ein 80-jahriger Patient stellte sich mit zunehmender Heiserkeit und Verschleimung vor. Lupenlaryngoskopisch zeigte sich eine deutliche Auftreibung des linken Taschenbandes mit Rotung ohne Epitheldefekt. Die Stroboskopie erbrachte eine unauffallige Stimmlippenfunktion bei geringer Glottisschlussinsuffizienz. Im MRT konnte die Gewebsformation ebenfalls dargestellt werden. Im Rahmen einer Panendoskopie wurde der Taschenbandbefund mikrochirurgisch abgetragen. Makroskopisch zeigte sich eine lipomatose Struktur. Pathohistologisch wurde das Gewebe als Choristom im Sinne einer Drusenheterotopie im Fett-/Bindegewebe des Taschenbandes eingestuft. Postoperativ ergaben sich keine Auffalligkeiten. Die Abklarung unklarer Larnyxbefunde sollte immer mikrolaryngoskopisch mit entsprechender Gewebegewinnung erfolgen. Der Befund einer benignen Gewebsheterotopie, die im vorliegenden Fall als Choristom eingestuft wurde, soll diese Empfehlung unterstutzen und die Differenzialdiagnose seltener Larynxtumoren erweitern.
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- 2005
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19. Retroperitoneal tumours: the pathologist's view
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S, Störkel
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Diagnosis, Differential ,Humans ,Sarcoma ,Retroperitoneal Neoplasms ,Retroperitoneal Space ,Neoplasm Grading ,Pathology, Molecular ,Neoplasm Staging - Abstract
Retroperitoneal lesions/sarcomas are rare. Diagnosis is difficult and needs special methods. Diagnosis and Treatment is an interdisciplinary process. Molecular Diagnostics will become more and more necessary in the future.
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- 2013
20. [T1 bladder cancer: role of documentation for bladder tumor findings and targeted second resection]
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D A, Lazica, S, Böttcher, S, Degener, F-C, von Rundstedt, A S, Brandt, S, Störkel, and S, Roth
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Male ,Reoperation ,Neoplasm, Residual ,Urology ,Documentation ,Middle Aged ,Medical Oncology ,Treatment Outcome ,Health Records, Personal ,Urinary Bladder Neoplasms ,Risk Factors ,Germany ,Practice Guidelines as Topic ,Prevalence ,Humans ,Female ,Aged - Abstract
For control resection of T1 bladder tumors an exact relocalization of the previously infiltrating tumor spread can be complicated by postreactive alterations, multiple scar tissue or change of surgeons. In this study the results of control transurethral resection of the bladder (TURB) after T1 high grade bladder tumors with the focus on localization and importance of standardized exact documentation were analyzed.From July to February 2012 a control resection was performed in 167 patients due to a T1 high grade bladder cancer. The rates of residual tumor tissue and localization were investigated with standardized tumor documentation.Out of 167 patients with T1 bladder cancer who underwent a control resection tumor tissue was found in 58.1 % (97 out of 167) and in 85.6 % (83 out of 97) the primary site was affected (41.2 % only at primary site and 44.3 % additionally at other locations). In 11 patients (11.3 %) residual tumor tissue at the initial site was only detected histologically.Our results indicate that T1 high grade bladder cancers show a relevant rate of residual tumor tissue at control resection which confirms the clinical guidelines of the European Association of Urology (EAU) on mandatory resection. In most cases the primary tumor site is affected. The standardized bladder tumor documetation allows well-directed control resection also in patients with multiple scars and post-TUR alterations, even when performed by a different surgeon.
- Published
- 2013
21. [Impact of operator experience on TURB of high-grade non-muscle-invasive bladder cancer--analysis of 254 second resections at a university teaching hospital]
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D A, Lazica, S, Degener, S, Böttcher, A S, Brandt, S, Störkel, and S, Roth
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Male ,Quality Control ,Urology ,Internship and Residency ,Cystoscopy ,Hospitals, University ,Physician Executives ,Physician Assistants ,Treatment Outcome ,Urinary Bladder Neoplasms ,Germany ,Humans ,Female ,Clinical Competence ,Guideline Adherence ,Neoplasm Grading ,Neoplasm Recurrence, Local ,Neoplasm Staging ,Retrospective Studies - Abstract
In departments with urological training of residents, part of the TURB procedures are performed as "teaching surgery". Does resection quality and early recurrence depend on the operator's experience?From July 2007 to February 2012 254 second resections (TURB) after Ta high-grade and T1 high-grade bladder tumours were performed at our institution. The surgeons were stratified into "junior residents" (first and second year of training), "experienced residents" (3rd-5th year of training), board certified urologists, consultants and chief surgeons. We analysed the risk of recurrence at second resection and characteristics of the initial TURB.87 patients presented with a Ta high-grade tumour (34.3%) and 167 had a T1 high-grade lesion (67.7%). Most TURBs were performed by "experienced residents" (3rd-5th year) and the chief of department. The recurrence rate at second resection was 52.4%. A significant association with the recurrence rate was shown for the number of initial tumours, size and T-stage. No association was found for the training level of the surgeon. Additionally, there was no different detrusor rate for the surgeons, as a parameter for a correct, muscle-deep TURB. A bias that surgeons in training had more favourable tumours (solitary, less than 3 cm) could be excluded.In our data detrusor rate and recurrence risk at second resection are independent of the surgeon's experience. The results of "teaching-TURBs" are not inferior compared to TURBs performed by board certified urologists or consultants under the conditions of undisturbed communication and personal supervision.
- Published
- 2013
22. [T1 high-grade bladder cancer - value of second operation with prognostuic parameters of first operation: analysis of 167 cases]
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D A, Lazica, S, Böttcher, S, Degener, F-C, von Rundstedt, A S, Brandt, S, Roth, S, Störkel, and M J, Mathers
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Male ,Neoplasms, Multiple Primary ,Reoperation ,Urinary Bladder Neoplasms ,Humans ,Female ,Cystoscopy ,Neoplasm Grading ,Neoplasm Recurrence, Local ,Cystectomy ,Prognosis ,Aged ,Neoplasm Staging - Abstract
We have evaluated the results of second transurethral resections of the bladder (TURB) after T1 high-grade bladder cancer over a 4.5-year period.From July 2007 to February 2012, 2172 TURB procedures were performed at our institution, of which 1130 were initial resections owing to primary tumour or relapse. Of these, 258 revealed T1 high-grade bladder cancer, and here we investigated tumour characteristics of the initial TURB and results of the second resection.The incidence of T1 high-grade tumours was 22.8% (N=258). Of 167 patients who underwent a second resection, tumour was found in 58.1% (97 of 167). Tumours were mostly multifocal (61.9%) and smaller than 3 cm (69.1%). Histology of the second resection revealed Ta low-grade in 8.4%, Ta high-grade in 16.2%, T1 high-grade in 19.8% and an upstaging to T2 and more in 6.6%. A significant association with the recurrence rate was found for the number of tumours at initial TURB: patients with multiple tumours at initial TURB had a recurrence rate of 69.0% compared with only 46.3% of patients with solitary tumour. For tumour-size and detrusor muscle in specimen a non-significant association was shown.T1 high-grade bladder cancers show a relevant rate of tumour at second TURB which confirms the clinical guidelines of the EAU. A significant association for a tumour-free second TURB in our data was shown for solitary tumours. A non-significant association was shown for tumour-size and when detrusor muscle was present in the specimen. Currently there is no data to determine the best time interval before second resection.
- Published
- 2013
23. Chromophilic renal cell carcinoma: cytomorphological and cytogenetic characterisation of four permanent cell lines
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Mario Sarbia, S. Störkel, Helmut E. Gabbert, Uwe Ramp, P. Koldovsky, Claus-Dieter Gerharz, B. Hildebrandt, and Roland Moll
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Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Cell ,Mice, Nude ,Vimentin ,Biology ,Mice ,Cytokeratin ,Renal cell carcinoma ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,Carcinoma, Renal Cell ,Aged ,Cell Cycle ,Cytogenetics ,DNA, Neoplasm ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,Clone Cells ,medicine.anatomical_structure ,Oncology ,Chromosome 3 ,Karyotyping ,Tetrasomy ,biology.protein ,Female ,Cell Division ,Clear cell ,Research Article - Abstract
Chromophilic renal cell carcinoma is a distinct type of human renal cancer, only recently recognised and defined by its characteristic histomorphological aspect and cytogenetic aberrations. We are the first to report on the establishment and cytogenetic characterisation of a panel of four permanent cell lines, i.e. chromphi-1, -2, -3 and -4, derived from strictly defined renal cell carcinomas (RCCs) of the chromophilic type and kept in continuous culture for up to 5 years. Immunohistochemistry revealed coexpression of vimentin and cytokeratins in all cell lines the cytokeratin polypeptide patterns, however, varying between the different cell lines. By light and transmission electron microscopy, various amounts of cytoplasmatic glycogen deposition were observed, being most pronounced in chromphi-3 and -4. The mean population doubling time ranged from 24 h (chromphi-1) to 51 h (chromphi-4). Chromphi-1 tumour cells produced slowly growing tumours in nude mice using the subrenal capsule assay. In all cell lines, cytogenetic analysis revealed numerical chromosomal aberrations known to be characteristic for chromophilic RCCs, i.e. loss of the Y chromosome, tri- or tetrasomy of chromosomes 7 and 17 as well as various combinations of additional structural and numerical chromosomal aberrations. Karyological aberrations were least pronounced in chromphi-2 and most complex in chromphi-1. Chromosomal aberrations typically affecting the short arm of chromosome 3 in clear cell RCCs were not observed in any of our cell lines. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5
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- 1996
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24. Standardisierte pathoanatomische Befunddokumentation auf den ADT-Tumorb�gen f�r Malignome des Mundes, der Kiefer und des Gesichts (Version III)
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H P Howaldt, G Herrmann, and S Störkel
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Lymphatic metastasis ,medicine.medical_specialty ,Pathology ,business.industry ,General surgery ,MEDLINE ,Cancer ,medicine.disease ,language.human_language ,Pathology and Forensic Medicine ,German ,Documentation ,language ,Prognostic model ,Medicine ,Neoplasm staging ,Observational study ,business - Abstract
The documentation form for pathohistologic findings (version III) for tumors of the maxillofacial region is presented. It is part of the site-specific documentation of the German Association of Tumor Centers (ADT). Its handling is explained by instructions which are based on the ICD-O classification and the TNM system. The prognostic relevance of the standardized documentation has already been proven by various histomorphologic investigations concerning oral and oropharyngeal cancer. By means of a multicentric observational study the German Austrian Swiss co-operative group DOSAK will develop a new prognostic model for oral and oropharyngeal cancer.
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- 1995
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25. Carcinoid in a horseshoe kidney
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Jw Oosterhuis, S. Störkel, E. van den Berg, B. de Jong, Trijnie Dijkhuizen, H.J.A. Mensink, and A.S.H. Gouw
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endocrine system ,Cancer Research ,Pathology ,medicine.medical_specialty ,Kidney ,Cytogenetics ,Horseshoe kidney ,Karyotype ,Dna index ,Wilms' tumor ,Anatomy ,Biology ,medicine.disease ,medicine.anatomical_structure ,Genetics ,medicine ,Immunohistochemistry ,neoplasms ,Molecular Biology ,Chromosome 13 - Abstract
Renal carcinoids are very rare neoplasms. We were able to culture and subsequently karyotype a carcinoid located in the isthmus of a horseshoe kidney, which revealed the following chromosomal pattern: 47,XX,+13[8]/46,XX,t(13;14)(q31;q11.2)[5]/46,XX[2]. The DNA index was 1. Our results, compared with the sparse data from the literature, suggest that carcinoid of the kidney has no cytogenetic aberrations in common with carcinoids from other anatomical sites reported. On the other hand, numerical and structural aberrations of chromosome 13 seem to play a crucial role in the development of metanephric-derived renal tumors.
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- 1995
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26. Chromosomal changes in renal oncocytomas Evidence that t(5;11)(q35;q13) may characterize a second subgroup of oncocytomas
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S. Störkel, Jw Oosterhuis, Trijnie Dijkhuizen, H.J.A. Mensink, E. van den Berg, B. de Jong, A. Dam, and G. Brutel de la Rivière
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Adenoma ,Male ,congenital, hereditary, and neonatal diseases and abnormalities ,Cancer Research ,Monosomy ,Pathology ,medicine.medical_specialty ,Chromosomal translocation ,Biology ,urologic and male genital diseases ,Y chromosome ,Translocation, Genetic ,Genetics ,medicine ,Humans ,Oncocytoma ,Molecular Biology ,Aged ,Chromosome Aberrations ,Chromosome 7 (human) ,Kidney ,Chromosomes, Human, Pair 11 ,Chromosome ,Cancer ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,medicine.anatomical_structure ,Karyotyping ,Chromosomes, Human, Pair 5 ,Female - Abstract
Many of the reported oncocytomas have different chromosome abnormalities, indicating that they comprise a cytogenetically heterogenous group of tumors consisting of potentially cytogenetic subgroups. We have performed cytogenetic studies on nine renal oncocytomas. Clonal abnormalities were present in eight tumors. The findings most observed were the loss of the Y chromosome, and abnormalities of chromosomes 1 and 22. We also observed telomeric associations (tas) in two tumors and structural aberrations of chromosomes 9p and 19q, as well as monosomy 10. In two cases we found a similar reciprocal t(5;11)(q35;q13) in two cases. Review of the literature disclosed one other oncocytoma with a t(5;11) (q35;q13). This suggests that t(5;11)(q35;q13) defines a (second) subset of oncocytomas apart from the subgroup specifically associated with the loss of chromosomes 1 and Y.
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- 1995
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27. EGFR expression as a predictor of survival for first-line chemotherapy plus cetuximab in patients with advanced non-small-cell lung cancer : analysis of data from the phase 3 FLEX study
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Rodryg Ramlau, Filippo de Marinis, Keunchil Park, José Rodrigues Pereira, Luis Paz-Ares, Joachim von Pawel, Maciej Krzakowski, Karl Maria Schumacher, Anja von Heydebreck, Ilhan Celik, Robert Pirker, Wilfried Eberhardt, Kenneth J. O'Byrne, and S. Störkel
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Oncology ,Male ,Lung Neoplasms ,Time Factors ,Medizin ,Cetuximab ,Kaplan-Meier Estimate ,Risk Factors ,Carcinoma, Non-Small-Cell Lung ,Antineoplastic Combined Chemotherapy Protocols ,Odds Ratio ,Medicine ,Prospective Studies ,Aged, 80 and over ,education.field_of_study ,Hazard ratio ,Antibodies, Monoclonal ,Vinorelbine ,Middle Aged ,Immunohistochemistry ,Up-Regulation ,ErbB Receptors ,Europe ,Survival Rate ,Treatment Outcome ,Biomarker (medicine) ,Female ,Brazil ,medicine.drug ,Adult ,medicine.medical_specialty ,Adolescent ,Population ,Antibodies, Monoclonal, Humanized ,Vinblastine ,Risk Assessment ,Disease-Free Survival ,Young Adult ,Internal medicine ,Republic of Korea ,Biomarkers, Tumor ,Humans ,Progression-free survival ,education ,Lung cancer ,Survival rate ,Protein Kinase Inhibitors ,Aged ,Proportional Hazards Models ,business.industry ,Patient Selection ,medicine.disease ,Cisplatin ,business - Abstract
Findings from the phase 3 First-Line ErbituX in lung cancer (FLEX) study showed that the addition of cetuximab to first-line chemotherapy significantly improved overall survival compared with chemotherapy alone (hazard ratio [HR] 0·871, 95% CI 0·762-0·996; p=0·044) in patients with advanced non-small-cell lung cancer (NSCLC). To define patients benefiting most from cetuximab, we studied the association of tumour EGFR expression level with clinical outcome in FLEX study patients.We used prospectively collected tumour EGFR expression data to generate an immunohistochemistry score for FLEX study patients on a continuous scale of 0-300. We used response data to select an outcome-based discriminatory threshold immunohistochemistry score for EGFR expression of 200. Treatment outcome was analysed in patients with low (immunohistochemistry score200) and high (≥200) tumour EGFR expression. The primary endpoint in the FLEX study was overall survival. We analysed patients from the FLEX intention-to-treat (ITT) population. The FLEX study is registered with ClinicalTrials.gov, number NCT00148798.Tumour EGFR immunohistochemistry data were available for 1121 of 1125 (99·6%) patients from the FLEX study ITT population. High EGFR expression was scored for 345 (31%) evaluable patients and low for 776 (69%) patients. For patients in the high EGFR expression group, overall survival was longer in the chemotherapy plus cetuximab group than in the chemotherapy alone group (median 12·0 months [95% CI 10·2-15·2] vs 9·6 months [7·6-10·6]; HR 0·73, 0·58-0·93; p=0·011), with no meaningful increase in side-effects. We recorded no corresponding survival benefit for patients in the low EGFR expression group (median 9·8 months [8·9-12·2] vs 10·3 months [9·2-11·5]; HR 0·99, 0·84-1·16; p=0·88). A treatment interaction test assessing the difference in the HRs for overall survival between the EGFR expression groups suggested a predictive value for EGFR expression (p=0·044).High EGFR expression is a tumour biomarker that can predict survival benefit from the addition of cetuximab to first-line chemotherapy in patients with advanced NSCLC. Assessment of EGFR expression could offer a personalised treatment approach in this setting.Merck KGaA.
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- 2012
28. Cellular immune response to human renal-cell carcinomas: Definition of a common antigen recognized by HLA-A2-restricted cytotoxic T-Lymphocyte (CTL) clones
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Christoph Huber, Karl-Hermann Meyer zum Büschenfelde, S. Störkel, Helga Bernhard, Thomas Wölfel, Catherine Wölfel, Alexander Knuth, Michael Stöckle, Julia Karbach, Petra Busch, and Barbara Seliger
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Cancer Research ,Lymphocyte ,Cross Reactions ,Biology ,Kidney ,Immune system ,Antigen ,Antigens, Neoplasm ,HLA-A2 Antigen ,Tumor Cells, Cultured ,medicine ,Humans ,Cytotoxic T cell ,Carcinoma, Renal Cell ,Melanoma ,Immunity, Cellular ,Histocompatibility Antigens Class I ,Antibodies, Monoclonal ,T lymphocyte ,Antigens, Differentiation ,Autologous tumor cell ,Kidney Neoplasms ,CTL ,medicine.anatomical_structure ,Oncology ,Immunology ,Lymphocyte Culture Test, Mixed ,Clone (B-cell biology) ,T-Lymphocytes, Cytotoxic - Abstract
Cytotoxic T lymphocyte (CTL) clones directed against autologous renal-cell carcinoma (RCC) cell lines were generated by mixed lymphocyte/tumor-cell culture (MLTC) using peripheral blood lymphocytes (PBL). A CD8+, CD4- CTL clone MZ1257-CTL 5/30 with high cytolytic activity for the autologous tumor cell line MZ1257-RCC was established. No lysis of the autologous EBV-transformed B lymphocytes (EBV-B) or K562 cells was observed. A panel of HLA-A2-matched allogeneic RCC lines was recognized by CTL 5/30. Further specificity analysis showed a cross-reactivity with HLA-A2-matched allogeneic tumor cells of various origins, especially melanoma. CTL 5/30 was also cross-reactive with several HLA-A2-positive allogeneic normal kidney cells in culture. The restriction element identified for CTL 5/30 was HLA-A2, as shown by blocking of cytotoxicity using an anti-HLA-A2 monoclonal antibody (MAb) and by resistance of an HLA-A2-negative melanoma variant SK29-MEL. 1.22 against lysis by CTL 5/30. In this report we demonstrate HLA-A2-restricted recognition of a T-cell-defined antigen on autologous renal-cancer cells. This antigen is also expressed and recognized in association with HLA-A2 on normal kidney cells in culture and other HLA-A2-positive tumor cells. It may therefore be a normal differentiation antigen to which tolerance is incomplete in the renal-cell cancer system investigated.
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- 1994
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29. Gegenüberstellung der Wertigkeit von Angioskopie, Angiographie und Sonographie zur Erfassung pathologischer Gefäßwandprozesse
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L. Happel, S. Störkel, Manfred Thelen, and Elke M. Voges
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medicine.medical_specialty ,medicine.diagnostic_test ,Vascular disease ,business.industry ,Angioscopy ,Interventional radiology ,medicine.disease ,Acoustic shadow ,Endoscopy ,Stenosis ,Angiography ,medicine ,Radiology, Nuclear Medicine and imaging ,Radiology ,business ,Vascular Stenosis - Abstract
AIMS The ability of angioscopy, angiography and sonography to evaluate arteriosclerotic vascular lesions were compared to determine if angioscopy is a valuable addition in the diagnostic armamentarium. METHODS An in-vitro study was selected to enable microscopic assessment of the arteries. Angiography was followed by sonography and endoscopy and finally the vessels were evaluated macro- and microscopically. RESULTS The configuration of the vascular stenosis (plaque-shape, semicircular-eccentric, circular-concentric) was more reliably defined with angioscopy as compared to angiography and sonography. Intense calcification of plaque was detected sonographically with a high sensitivity (90%) using the acoustic shadowing and high amplitude criteria. Angioscopy was superior in differentiating between simple and complicated plaques and the assessment of ulcerations, while the visualization of the entire vascular tree with potential collaterals remained the domain of angiography. CONCLUSIONS Angioscopy seems to be valuable addition in the evaluation of arterial vascular disease. It can be used in the quality control after interventional radiology and surgery.
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- 1994
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30. Membranes Activate Tumor- and Virus-Specific Precursor Cytotoxic T Lymphocytes In Vivo and Stimulate Tumor-Specific T Lymphocytes In Vitro
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Pramod K. Srivastava, T. Wölfel, S. Störkel, Michael Heike, K.-H. Meyer Zum Büschenfelde, and Nathalie E. Blachere
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Cancer Research ,Cellular immunity ,Immunology ,chemical and pharmacologic phenomena ,Lymphocyte Activation ,Major histocompatibility complex ,Cell Line ,Mice ,Antigen ,Neoplasms ,MHC class I ,Animals ,Immunology and Allergy ,Cytotoxic T cell ,Pharmacology ,Mice, Inbred C3H ,biology ,Cell Membrane ,Histocompatibility Antigens Class I ,Vaccination ,T lymphocyte ,Hematopoietic Stem Cells ,Virology ,Cell biology ,CTL ,Membrane ,Viruses ,biology.protein ,T-Lymphocytes, Cytotoxic - Abstract
Plasma membranes contain the entire antigenic repertoire of a cell in the form of processed antigens presented as peptides by major histocompatibility complex (MHC) class I molecules. We report here that plasma membranes but not internal membranes of cognate tumors stimulate murine fibrosarcoma and human melanoma-specific cytotoxic T lymphocyte (CTL) clones in vitro in an antigen-specific. MHC class I-restricted manner. Although stimulation of CTLs by class I-peptide complexes on reconstituted membranes has been documented before, this is the first demonstration of stimulation of cloned CTLs by natural, endogenously processed MHC class I-peptide complexes on plasma membranes. In addition to their ability to stimulate CTLs in vitro, immunization of syngeneic mice with membranes derived from ultraviolet-induced tumor cells, SV40 transformed fibroblasts, or influenza-infected fibroblasts elicits an antigen-specific, MHC class I restricted primary CTL response. To the best of our knowledge, this is also the first demonstration of the ability of cellular membranes to prime an MHC class I-restricted CTL responses in vivo. The ability of membranes to prime a CTL response in vivo suggests that they may be used as T-cell vaccines against tumors or infectious viruses. This approach circumvents the difficulties in generation of human tumor cell lines and identification of CTL-recognized determinants for vaccination and avoids some of the risks associated with whole-cell vaccination such as inoculation of patients with immunosuppressive factors, transforming DNA, or infectious viruses.
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- 1994
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31. Factors influencing survival after resection of pancreatic cancer. A DNA analysis and a histomorphologic study
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S. Störkel, Michael Stöckle, Theo Junginger, Stefan Wellek, and Thomas C. Böttger
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Male ,Cancer Research ,medicine.medical_specialty ,Pathology ,Pancreatic disease ,Multivariate analysis ,Gastroenterology ,Polyploidy ,Pancreatectomy ,Text mining ,Pancreatic cancer ,Internal medicine ,medicine ,Humans ,Stomach cancer ,Lymph node ,Neoplasm Staging ,Cell Nucleus ,Univariate analysis ,business.industry ,DNA, Neoplasm ,Prognosis ,medicine.disease ,Diploidy ,Pancreatic Neoplasms ,Survival Rate ,medicine.anatomical_structure ,Oncology ,Lymphatic Metastasis ,Multivariate Analysis ,Lymph Node Excision ,Female ,Pancreas ,business ,Follow-Up Studies - Abstract
BACKGROUND The influence of DNA content on prognosis in stomach cancer has been investigated rarely, and the results are controversial. METHOD The prognostic relevance of the DNA content and histomorphologic parameters was evaluated in 41 patients after resection of pancreatic cancer. RESULTS In the univariate analysis, the DNA content, tumor size, lymph node status, tumor stage, nuclear grade, and type of resection had a statistically significant influence on the prognosis. No association was found between the DNA content and the histomorphologic features. Apart from the operative procedure, the DNA content was the strongest indicator of prognosis in the multivariate analysis. CONCLUSIONS Further investigations are necessary to find out if DNA analysis can be performed preoperatively on material obtained by fine-needle aspiration.
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- 1994
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32. Cytogenetic analysis of epithelial renal-cell tumors: Relationship with a new histopathological classification
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A. Dam, Trijnie Dijkhuizen, H. J. A. Mensink, E. van den Berg, Jw Oosterhuis, Charles H.C.M. Buys, H. M. M. Zweers, S. Störkel, AH van der Hout, B. de Jong, and Faculteit Medische Wetenschappen/UMCG
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Cancer Research ,Pathology ,medicine.medical_specialty ,Monosomy ,Cell type ,CARCINOMA ,Chromosome Disorders ,Histogenesis ,Biology ,Polysomy 7 ,Loss of heterozygosity ,medicine ,Humans ,Carcinoma, Renal Cell ,Chromosome Aberrations ,Chromosome 7 (human) ,Polysomy ,Ploidies ,ABNORMALITIES ,Cytogenetics ,DNA, Neoplasm ,medicine.disease ,Kidney Neoplasms ,ONCOCYTOMAS ,Oncology ,TISSUE ,Karyotyping - Abstract
Renal-cell carcinomas (RCC) are clinically, histologically and cytogenetically very heterogeneous. The present histological WHO classification shows no clear correlation between histologic subtypes and specific chromosomal abnormalities. In 1986, a new classification was proposed by Thoenes and Storkel based on the cell type from which the tumor arises. They distinguish S cell types: clear-cell, chromophilic, chromophobic, ductus Bellini and oncocytic. Results of 105 primary tumors show that, in this new classification, there is a correlation between different subtypes of renal-cell tumor and specific chromosomal abnormalities at a microscopic and/or molecular level. The clear-cell compact type shows structural aberrations of chromosomes 1, 3, 4, 5q, 6, 10q, 11q and 12q, together with polysomy of chromosomes X, 4, 5, 7, 10, 12, 15, 16, 19, 20, 21 and 22, monosomy of chromosomes 3, 8, 9, 13, 14, and loss of Y. The main characteristics of the chromophilic tubulo-papillary type are trisomies 7 and 17, and loss of the Y-chromosome. Chromophobic carcinoma seems to be correlated with, inter alia, polysomy 7, trisomies 12, 16, 18, 19, structural abnormalities of 11q, and telomeric associations. Oncocytomas do not reveal any specific chromosomal anomaly, except for trisomy 7. Loss of heterozygosity on 3p is only found in the clear-cell compact type. Some specific chromosomal abnormalities correlate with a particular grade of the tumor. These correlations support the hypothesis that specific chromosomal abnormalities play a role in the histogenesis and oncogenesis of RCC. They may be important for tumor diagnosis and clinical prognosis. (C) 1993 Wiley-Liss, Inc.
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- 1993
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33. Loss of heterozygosity at the short arm of chromosome 3 in renal‐cell cancer correlates with the cytological tumour type
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S. Störkel, Jw Oosterhuis, B. de Jong, Trijnie Dijkhuizen, P. van der Vlies, AH van der Hout, E. van den Berg, and Chcm Buys
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Heterozygote ,Cancer Research ,medicine.medical_specialty ,Pathology ,Mitotic crossover ,CARCINOMA ,Chromosome Disorders ,Biology ,MOLECULAR ANALYSIS ,Loss of heterozygosity ,Gene duplication ,medicine ,Humans ,Carcinoma, Renal Cell ,Sequence Deletion ,Chromosome Aberrations ,DELETION ,Breakpoint ,Cytogenetics ,Chromosome ,CYTOGENETICS ,Kidney Neoplasms ,Oncology ,Chromosome 3 ,Clear cell carcinoma ,Chromosomes, Human, Pair 3 ,Polymorphism, Restriction Fragment Length - Abstract
A majority of renal-cell tumours retain heterozygosity at the short arm of chromosome 3. To investigate possible histopathological differences between tumours with and without such losses, we compared loss of heterozygosity data from 51 tumours with 1 histological and 2 different cytological classifications of renal-cell tumour. Using the cytological classification of Thoenes et al., we only found tumours with loss of heterozygosity in these authors' clear-cell category. Possibly, only these tumours arise by a mechanism of double loss of a tumour-suppressor gene on 3p, non-clear-cell renal tumours having a different genetic background. Alternatively, deletions may occur in all subtypes, in which case those subtypes in which no LOH is found may also contain deletions too small to be detected with the set of 3p probes we used. A cytogenetic analysis was carried out on 30 of the tumours. Results of molecular and microscopic cytogenetic analyses did not seem to be in agreement in 12 cases. In 6 of these we found allelic losses in tumours showing morphologically normal copies of chromosome 3. Mitotic recombination or loss of one chromosome 3 homologue followed by duplication of the remaining homologue is a likely explanation. The other 6 cases showed microscopic abnormalities of chromosome 3 which were not reflected, or only partly reflected, as allelic losses. These discrepancies are caused either by the limitations of microscopic analysis in exactly determining a breakpoint or tracing a translocated part of a chromosome, or by the failure of molecular analysis to demonstrate LOH if this occurs in only a minority of cells.
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- 1993
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34. Fournier-Gangrän als seltene Komplikation nach Staplerhämorrhoidektomie Kasuistik und Literaturübersicht
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P. Prohm, Ch. Bönner, and S. Störkel
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Gynecology ,medicine.medical_specialty ,business.industry ,medicine ,Surgery ,business - Published
- 2001
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35. Contents Vol. 93, 2001
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T. Brueckmann, W. Brenner, M. Steinemann, W. Vogel, S. Schlaubitz, C. Zühlke, M. Lombard, F. Boán, K. Benirschke, S. Naumann, S.W. Bremer, C. Steinlein, S. Steinemann, F. Richard, P.D. Thomsen, M. Yerle, K.D. Zang, Z. Docherty, C. Amid, K. Mrasek, I. Schubert, M. Mende, I. Nanda, T. Paiss, C. Genêt, L.J. Peelman, I. Chudoba, M. Hughes, R.-D. Wegner, U. Claussen, L. Sánchez, B. Seipel, F. Grützner, F.J. García-Cozar, D. Prawitt, B.U. Zabel, J.L. Wright, A. Van Zeveren, K. Stout, V. Kalscheuer, M. Stumm, R.V. Rambau, N. Reissmann, D.S. Gallagher, B. Zabel, A. Ishikawa, C. Messaoudi, A.T. Kumamoto, E.C. Akeson, A. Mujica, A. Dalski, P. Kaiser, T. Liehr, J.G. Scammell, S. Bremer, C. Pfeifer, S. Munsche, M.M. Valdivia, M. Van Poucke, M. Schmid, C.M. Tuck-Muller, H. Starke, F. Domínguez, Y. Matsuda, S. Störkel, C.G. Mathew, F.F.B. Elder, S. Narayanswami, H. Scherthan, J. Decker, E. Schwinger, A. Niveleau, V. Trifonov, H. Mayrhofer, J. Gómez-Márquez, J.P. Lambert, S.-E. Bikar, E. Zend-Ajusch, L.J. Bechtel, T. Haaf, Y.A. Wang, A. Viñas, C. Iglesias, C. Mackie Ogilvie, A. Bahr, T. Nagase, A. Dufke, H.H.Q. Heng, A. Winterpacht, W. Lu, T.J. Robinson, C. Maier, K. Matsubara, A. Heller, A. Kuroiwa, M. Rocchi, B. Dutrillaux, C.J. Ye, N. Nomura, N. Rubtsov, E.R. Schmidt, T. Namikawa, M.T. Davisson, C. Tuggle, K. Gardiner, H. Enders, G. Liu, M. Buceta, H. Hanson, H. Hauser, N. Sampson, H. Neitzel, P.D. Waters, T. Hankeln, H. Tönnies, C. Pendón, J. Bolívar, M.L. Houck, D. Reutzel, M. Leipoldt, A. Cichutek, P. Moens, J.A.M. Graves, P.J. Kirby, B. Maurer, A. Astola, S.A. Krawetz, and F. Piumi
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Botany ,Genetics ,Biology ,Molecular Biology ,Genetics (clinical) - Published
- 2001
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36. Alpha-Fetoprotein-Producing Renal Cell Carcinoma
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Ulrich Witzsch, R.R. de Vries, V. Poulakis, Hans-Michael Altmannsberger, S. Störkel, and E. Becht
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Urology ,CA 15-3 ,urologic and male genital diseases ,Renal cell carcinoma ,medicine ,Carcinoma ,Humans ,Carcinoma, Renal Cell ,neoplasms ,Tumor marker ,business.industry ,digestive, oral, and skin physiology ,Bone metastasis ,medicine.disease ,Kidney Neoplasms ,digestive system diseases ,embryonic structures ,alpha-Fetoproteins ,business ,Alpha-fetoprotein ,Liver cancer ,Kidney disease - Abstract
Alpha-fetoprotein (AFP) is recognized as a tumor marker of yolk sac tumors, liver cancer and some other cancers of the digestive organs. Renal cell carcinoma (RCC) producing AFP is a rare entity. A case of AFP-producing RCC with solitary bone metastasis, but without liver involvement, is reported. The stain specific to AFP proved the presence of AFP in the cytoplasms of more cells of the renal tumors. Additionally, the other published cases are reviewed. These cases indicate that mesoderm-originating malignant tumors such as RCCs can produce AFP in some situations. So, AFP is probably more universal than believed, although it is generally a popular and useful tumor marker for hepatocellular carcinomas and yolk sac tumors.
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- 2001
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37. [Renal cell carcinomas. Pathomorphologic diagnosis in the light of modern pharmacotherapy]
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S, Störkel
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Drug Delivery Systems ,Molecular Diagnostic Techniques ,DNA Mutational Analysis ,Biomarkers, Tumor ,Antibodies, Monoclonal ,Humans ,Antineoplastic Agents ,Protein-Tyrosine Kinases ,Kidney ,Prognosis ,Carcinoma, Renal Cell ,Kidney Neoplasms ,Signal Transduction - Abstract
Renal carcinomas have been classified with increasing accuracy in recent years and new sub-groups have been assigned, which, due to their distinct development pathways, carry varying prognoses and require specific treatment approaches. A range of antibodies are available for their immunohistochemical classification. Identifying a gene defect--mutation, deletion, trisomies and monosomies, oncogene activation--enables the application of targeted therapy, e.g. using antibodies or tyrosine kinase inhibitors in signaling pathways, among others. Renal carcinoma diagnosis by the pathologist is taking on a particularly important role.
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- 2010
38. Autosomal dominant polycystic kidney disease—in vitro culture of cyst-lining epithelial cells
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K H Meyer zum Büschenfelde, Hans Köhler, Reinhard Klingel, H. J. Rumpelt, S. Störkel, Wolfgang Dippold, and Roland Moll
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Genetic Markers ,Pathology ,medicine.medical_specialty ,Autosomal dominant polycystic kidney disease ,HLA-C Antigens ,Biology ,Epithelium ,Genetic linkage ,medicine ,Humans ,Northern blot ,Gene ,Cells, Cultured ,HLA-A Antigens ,PKD1 ,urogenital system ,Antibodies, Monoclonal ,General Medicine ,Middle Aged ,Blotting, Northern ,Polycystic Kidney, Autosomal Dominant ,medicine.disease ,Immunohistochemistry ,Molecular biology ,Phenotype ,Pedigree ,Blot ,Microscopy, Electron ,HLA-B Antigens ,Cell culture ,Female ,Chromosomes, Human, Pair 16 - Abstract
The major form of autosomal dominant polycystic kidney disease (ADPKD) in humans is linked to the PKD1 gene on chromosome 16p. The identity of the gene and the underlying pathogenetic mechanisms are not yet defined. Cyst-lining epithelial cells derived from a polycystic kidney were successfully grown in culture and designated MZ-PKD-1 cells. By linkage analysis, the related pedigree of the nephrectomized patient could be linked to the PKD1 gene on chromosome 16p. Thus, these cells exhibit the genotype of a mutated PKD1 gene and represent an in vitro culture model for ADPKD involving chromosome 16p. The antigenic phenotype was characterized immunohistologically by epithelial differentiation antigens and markers of individual nephron segments. An essentially identical antigenic pattern of proximal tubular cells was observed both in vitro and in fresh frozen tissue. Electron microscopy showed the formation of a microvillous-like coating. During growth phases in vitro successive changes in the cell shape were observed. MZ-PKD-1 cells exhibited a limited lifespan ending in replicative senescence. Northern blot analysis of kidney-growth-related genes, c-myc, TGF-alpha, TGF-beta 1, and EGF receptor revealed abundant expression of all of these genes in MZ-PKD-1 cells.
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- 1992
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39. The role of osteonectin in human tooth development: An immunohistological study
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Larry W. Fisher, S. Störkel, T. Reichert, and K. Becker
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Adult ,Pathology ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,Dentistry ,Stratum intermedium ,Fetus ,Endocrinology ,stomatognathic system ,Amelogenesis ,Human tooth development ,medicine ,Humans ,Osteonectin ,Orthopedics and Sports Medicine ,Child ,Dental Cementum ,biology ,business.industry ,Chemistry ,Infant, Newborn ,Infant ,Dentinogenesis ,Fibroblasts ,musculoskeletal system ,Immunohistochemistry ,Cementogenesis ,stomatognathic diseases ,Odontoblast ,Child, Preschool ,biology.protein ,business ,Ameloblast ,Tooth - Abstract
We investigated immunohistologically 160 teeth and dental germs in various stages of tooth development taken from human individuals (13th week of pregnancy to the 24th year of life) to study the osteonectin expression in dental hard tissue. In the course of dentinogenesis, the predentin, the odontoblasts, and their cell processes show a positive osteonectin staining reaction. During cementogenesis, osteonectin is synthesized by cement-producing fibroblasts, cementoblasts, and cementocytes. The expression of osteonectin during dentinogenesis and cementogenesis is closely related to the development of the respective calcified tissue. All cells of the inner and outer enamel epithelium, the cells of the stratum reticulare and stratum intermedium, the ameloblasts, and the enamel substance are osteonectin negative, just as dentin and cement are. The results of this study indicate one important physiological role of osteonectin as a protein associated with the formation of collagen containing mineralizing tissues like human bone, as well as human dentin and cement.
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- 1992
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40. Characterization of conservatively resected renal tumors using automated image analysis DNA cytometry
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R. Mielke, H. El-Damanhoury, R. Hohenfellner, S. Störkel, Michael Stöckle, G. Voges, and F. Steinbach
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Cancer Research ,Pathology ,medicine.medical_specialty ,Angiomyolipoma ,Analysis dna ,Cell ,Renal cell adenoma ,Biology ,medicine.disease ,chemistry.chemical_compound ,medicine.anatomical_structure ,Oncology ,chemistry ,medicine ,Stem cell line ,Cytometry ,DNA - Abstract
The DNA histograms of 57 conservatively resected renal tumors were studied using automated image analysis DNA cytometry (Leytas II). Forty-nine of the analyzed tumors were renal cell carcinomas, six were oncocytomas, one was an angiomyolipoma, and one was a renal cell adenoma. On the basis of their DNA histograms, diploid, tetraploid, and aneuploid tumors could be distinguished. Aneuploid tumors could be subtyped further according to the DNA content of the stem cell line as hyperdiploid, hypertriploid, or hypertetraploid. Eight of the tumors were characterized by a combination of diploid and hypertriploid stem cell lines. During a mean follow-up of 5 years, only the two patients with a pure hypertriploid tumor died of distant metastases. These results indicate that automated DNA image analysis cytometry is able to differentiate among several types of renal tumors with obviously different prognoses.
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- 1991
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41. Computertomographie nach extrakorporaler Stoßwellenlithotripsie (ESWL) der Nieren
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M. Stadtbäumer, S. Störkel, T. Schaub, H. El-Damanhoury, H. J. Hennes, M. Kunisch, H. H. Schild, and Manfred Thelen
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Kidney ,medicine.medical_specialty ,Renal stone ,medicine.diagnostic_test ,business.industry ,Computed tomography ,Pig model ,Extracorporeal ,Lesion ,Extracorporeal shockwave lithotripsy ,medicine.anatomical_structure ,Positron emission tomography ,medicine ,Ct technique ,Radiology, Nuclear Medicine and imaging ,Radionuclide imaging ,Ct findings ,Radiology ,medicine.symptom ,business - Abstract
In an experimental study on pigs, 28 stone-free kidneys were treated with a second generation lithotripter. Histologically, acute bleeding was seen after ESWL in 23 (82%) of the 28 treated kidneys. CT studies before and after contrast were correct in 20/28 (74%) cases. It was shown that extracorporeal shock waves cause renal bleeding that is only partially detected by computed tomography. It can be concluded that the CT findings seen in a high percentage of renal stone patients after ESWL represent bleeding. The incidence of renal bleeding after ESWL and thus the possible risk of hypertension has obviously been underestimated so far in clinical studies.
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- 1991
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42. Long-Term Experience with Conservative Surgery of Renal Tumors
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F. Steinbach, M. Stöckle, J.W. Thüroff, S. Störkel, S.W. Melchior, R. Kiewel, S.C. Müller, and R. Hohenfellner
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Nephrology ,Urology - Published
- 1991
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43. Parameters Influencing Renal Damage in Extracorporeal Shock Wave Lithotripsy: An Experimental Study in Pigs
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M. Kunisch, T. Schaub, R. Hohenfellner, S. Störkel, H. Schild, M. Thelen, H. El-Damanhoury, and M. Stadtbäumer
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Shock wave ,medicine.medical_specialty ,Kidney ,Renal damage ,business.industry ,Urology ,medicine.medical_treatment ,Lithotripsy ,Extracorporeal shock wave lithotripsy ,Surgery ,Animal model ,medicine.anatomical_structure ,medicine ,Complication ,business - Abstract
Domestic pigs were used as an animai model in experiments involving detailed pathologic examination immediately after extracorporeal shock wave lithotripsy (SWL) in-order to study the effects of sh...
- Published
- 1991
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44. Acute and Chronic Renal Damage in Extracorporeal Shock Wave Lithotripsy: An Experimental Study in Pigs Correlating Ultrasound with Histopathology*
- Author
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R. Hohenfellner, M. Stadtbäumer, T. Schaub, H. El-Damanhoury, H. Schild, S. Störkel, M. Thelen, M. Kunisch, and K. Pfitzner
- Subjects
Kidney ,medicine.medical_specialty ,business.industry ,Urology ,medicine.medical_treatment ,Ultrasound ,Renal Hemorrhage ,Lithotripsy ,medicine.disease ,Extracorporeal shock wave lithotripsy ,Surgery ,medicine.anatomical_structure ,Hematoma ,Medicine ,Histopathology ,Radiology ,business ,Complication - Abstract
Using 18 domestic pigs, we investigated the value of real-time sonography for the determination of renal lesions immediately and 3 weeks after extracorporeal shock wave lithotripsy (SWL) on a lithotripter with electromagnetic shock wave generation. All animals were treated under general anesthesia with 2000 shock waves at 40 MPa. Eight animals (Group 1) were sacrificed after the ultrasound examination. Ten animals (Group 2) had a sonographic reexamination after 3 weeks and were sacrificed thereafter. Axial slices of the kidney specimens were prepared, and the presence and location of hematoma were noted. In Group 1 (acute damage), ultrasound showed renal damage in six kidneys. According to the histopathologic findings, six ultrasound studies were correct, one was false-positive, and one was false-negative. In Group 2, nine kidneys showed pathologic findings by ultrasound immediately after SWL. At 3-week reexamination, only one was still abnormal, but this kidney showed no renal hemorrhage on histopatholog...
- Published
- 1991
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45. Klassifikation der Nierenzellkarzinome/Tumoren und ihre Beziehung zum Nephron-Sammelrohrsystem
- Author
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S. Störkel, W. Thoenes, and Rumpelt Hj
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Cell type ,Pathology ,medicine.medical_specialty ,General Medicine ,Chromophobe cell ,Biology ,urologic and male genital diseases ,medicine.disease ,Drug Discovery ,Carcinoma ,medicine ,Molecular Medicine ,Intercalated Cell ,Oncocytoma ,Genetics (clinical) - Abstract
After a controversial phase of nomenclature (including--among others--the terms "hypernephroma" and "hypernephroid carcinoma") a cytomorphologically defined subtyping of renal cell tumours (adenomas, carcinomas, oncocytomas) is offered, based on new electron microscopical and histochemical observations. These data are in part supported by cytogenetical findings reported in the literature. Phenotypical/histogenetical relations to different parts or cell types, respectively, of the nephron-collecting duct system could be demonstrated. Chromophobe cell carcinoma and oncocytoma exhibit features of the intercalated cells.
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- 1990
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46. [Pathology in Wuppertal]
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S, Störkel
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Germany ,Pathology ,History, 20th Century - Published
- 2007
47. ['Retroperitoneal Fibrosis' (RPF) Urologic Cooperation and Research Project]
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A S, Brandt, S B, Soares, A, Fehr, S, Kukuk, M J, Mathers, S, Störkel, H, Lerch, A, Bockisch, P, Haage, R, Vollmann, P, Thürmann, and S, Roth
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Diagnostic Imaging ,Dose-Response Relationship, Drug ,Prednisolone ,Retroperitoneal Fibrosis ,Comorbidity ,Drug Administration Schedule ,Autoimmune Diseases ,Tamoxifen ,Germany ,Humans ,Interdisciplinary Communication ,Prospective Studies ,Registries ,Randomized Controlled Trials as Topic - Published
- 2007
48. [Villous adenoma of the renal pelvis and ureter]
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H, Frickmann, S, Jungblut, J, Bargon, K, Willenbrock, S, Störkel, and P, Hanke
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Aged, 80 and over ,Ureteral Neoplasms ,Nephrectomy ,Kidney Neoplasms ,Diagnosis, Differential ,Neoplasms, Multiple Primary ,Adenoma, Villous ,Humans ,Female ,Kidney Pelvis ,Ureter ,Tomography, X-Ray Computed ,Carcinoma in Situ ,Ultrasonography - Abstract
Villous adenomas of the urinary tract are extremely rare tumours belonging to the adenoepithelial metaplasias. They can be associated with other neoplasias, especially with carcinomas. We describe the case of an 85-year-old female patient suffering from a villous adenoma of the renal pelvis and ureter.
- Published
- 2006
49. Kasuistik: Villöses Adenom des Nierenbeckens und Ureters
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S. Störkel, S. Jungblut, H. Frickmann, P. Hanke, J. Bargon, and K. Willenbrock
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Urology - Published
- 2006
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50. Typing, Grading und Staging beim Harnblasenkarzinom: Pathomorphologische Parameter und deren Bedeutung für die Klinik
- Author
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S. Störkel
- Abstract
Typing, Staging und Grading stellen essentielle Parameter der pathologisch-anatomischen Begutachtung von Urothelkarzinomen dar, die ihrerseits als Grundlage fur nachfolgende klinische Entscheidungen und Therapieoptionen dienen. Mit der neuen WHO-Klassifikation der Harnblasenkarzinome (Eble et al. 2004) wurden erstmals nichtinvasive und invasive Urothelkarzinome unterschieden, weiterhin neue Entitaten eingefuhrt und die Nomenklatur vereinheitlicht. Auch das Grading von Urothelkarzinomen wurde neu gestaltet und auf nichtinvasive Tumoren beschrankt. Beim Staging erbringen Substaging-Vorschlage und neue mikroskopische Analyseansatze mehr Information fur den Urologen. Die nachfolgenden Ausfuhrungen sollen deshalb ein kurzgefasstes Update der sich wandelnden pathomorphologischen Befundung von Harnblasenkarzinomen geben.
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- 2005
- Full Text
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