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Abstract In Argentina, migratory activity in search of floral diversity has become a common approach to maximizing honey production. The Entre Ríos province possesses a floral diversity that allows beekeepers to perform migratory or stationary management. Beyond the impact caused by transhumance, migratory colonies in this province start and end the season in monoculture areas. To study the effect of these practices on viral infection, we assayed for the presence, abundance and genetic characterization of the Deformed Wing Virus (DWV) in honey bees from apiaries with both types of management. In migratory apiaries, DWV was detectable in 86.2% of the colonies at the beginning of the season (September 2018), and 66% at the end of the season (March 2019). On the other hand, DWV was detected in 44.11% and 53.12% of stationary samples, at the beginning and the end of the season, respectively. Sequence analysis from migratory and stationary colonies revealed that all samples belonged to DWV-A type. The highest viral loads were detected in migratory samples collected in September. Higher DWV presence and abundance were associated with migratory management and the sampling time. Based on our findings we propose that the benefit of migration to wild flowering areas can be dissipated when the bee colonies end the season with monoculture.

In the modern world, dams and the artificial reservoirs behind them serve the increasing demand for water across diverse needs such as agriculture, energy production, and drinking water. As dams continue to proliferate, monitoring water availability influenced by reservoir operations is now of paramount importance. The Reservoir Assessment Tool (RAT) is a data-driven software platform that integrates satellite remote sensing with hydrological models, enabling the estimation of key reservoir parameters such as inflow, outflow, surface area, evaporation, and storage changes. The earliest version of RAT (version 1.0) was set up for 1598 reservoirs around the world with limitations in functional robustness, updating frequency, and scalability. Some of these limitations on updating frequency and functional robustness were addressed in version 2.0 that was later made operational for the intergovernmental agency of the Mekong River Commission. Recognizing the need for scalability to mobilize the global water management community to benefit from satellite remote sensing, we hereby introduce RAT version 3.0. This version is optimized for accelerating open collaboration among users for continuous improvement and customization of RAT to enable reservoir management breakthroughs. RAT 3.0 represents a wholesale overhaul from the previous versions to empower the global community of users and developers in the spirit of the open-source movement. RAT 3.0 allows reservoir monitoring advancements and new functional developments that can be freely exchanged and seamlessly integrated for continuous evolution of the software. A centralized web application has also been established to facilitate the storage and dissemination of global reservoir monitoring information along with comprehensive training resources. RAT 3.0 aspires to bridge the traditional practices of water management community with the capabilities of satellite remote sensing. The global impact of the software can be expected to increase as uptake spreads, enabling a more sustainable and equitable utilization of our planet's water resources.

The global ocean's oxygen content has declined significantly over the past several decades and is expected to continue decreasing under global warming, with far-reaching impacts on marine ecosystems and biogeochemical cycling. Determining the oxygen trend, its spatial pattern, and uncertainties from observations is fundamental to our understanding of the changing ocean environment. This study uses a suite of CMIP6 Earth system models to evaluate the biases and uncertainties in oxygen distribution and trends due to sampling sparseness. Model outputs are sub-sampled according to the spatial and temporal distribution of the historical shipboard measurements, and the data gaps are filled by a simple optimal interpolation method using Gaussian covariance with a constant e-folding length scale. Sub-sampled results are compared to full model output, revealing the biases in global and basin-wise oxygen content trends. The simple optimal interpolation underestimates the modeled global deoxygenation trends, capturing approximately two-thirds of the full model trends. The North Atlantic and subpolar North Pacific are relatively well sampled, and the simple optimal interpolation is capable of reconstructing more than 80 % of the oxygen trend in the non-eddying CMIP models. In contrast, pronounced biases are found in the equatorial oceans and the Southern Ocean, where the sampling density is relatively low. The application of the simple optimal interpolation method to the historical dataset estimated the global oxygen loss to be 1.5 % over the past 50 years. However, the ratio of the global oxygen trend between the sub-sampled and full model output has increased the estimated loss rate in the range of 1.7 % to 3.1 % over the past 50 years, which partially overlaps with previous studies. The approach taken in this study can provide a framework for the intercomparison of different statistical gap-filling methods to estimate oxygen content trends and their uncertainties due to sampling sparseness.

Abstract Isotopic analyses of prehistoric diet have only recently reached the threshold of going beyond site-focused reports to provide regional syntheses showing larger trends. In this work we present the first regional analysis for Neolithic southeastern Italy as a whole, including both substantial original data and a review of the available published data. The results show that dietary isotopes can shed new light on a number of traditional and important questions about Neolithic foodways. First, we observe regional variations in the distribution of stable isotope values across the area, suggesting variability in the Neolithic diet. Secondly, we show that, although the plant food calorific intake was primary for these communities, animal products were also important, representing on average 40% of the total calories. Third, we note that marine fish was only minorly consumed, but that this could be an underestimation, and we observe some variability in the regions considered, suggesting differences in local human–environment interactions. People in different regions of southeastern Italy may have consumed different versions of a common Neolithic diet. Regional synthesis also allows us to take stock of gaps and new directions in the field, suggesting an agenda for Neolithic isotopic research for the 2020s.

No-tillage is often suggested as a strategy to reduce greenhouse gas emissions. Modeling tillage effects on nitrous oxide (N2O) emissions is challenging and subject to great uncertainties as the processes producing the emissions are complex and strongly nonlinear. Previous findings have shown deviations between the LPJmL5.0-tillage model (LPJmL: Lund–Potsdam–Jena managed Land) and results from meta-analysis on global estimates of tillage effects on N2O emissions. Here we tested LPJmL5.0-tillage at four different experimental sites across Europe and the USA to verify whether deviations in N2O emissions under different tillage regimes result from a lack of detailed information on agricultural management, the representation of soil water dynamics or both. Model results were compared to observational data and outputs from field-scale DayCent model simulations. DayCent has been successfully applied for the simulation of N2O emissions and provides a richer database for comparison than noncontinuous measurements at experimental sites. We found that adding information on agricultural management improved the simulation of tillage effects on N2O emissions in LPJmL. We also found that LPJmL overestimated N2O emissions and the effects of no-tillage on N2O emissions, whereas DayCent tended to underestimate the emissions of no-tillage treatments. LPJmL showed a general bias to overestimate soil moisture content. Modifications of hydraulic properties in LPJmL in order to match properties assumed in DayCent, as well as of the parameters related to residue cover, improved the overall simulation of soil water and N2O emissions simulated under tillage and no-tillage separately. However, the effects of no-tillage (shifting from tillage to no-tillage) did not improve. Advancing the current state of information on agricultural management and improvements in soil moisture highlights the potential to improve LPJmL5.0-tillage and global estimates of tillage effects on N2O emissions.

The improvement of the fracture toughness of adhesive joints is a key factor in many structural applications. The ability of nylon electrospun nanofibrous mat to act as an adhesive carrier and reinforcing web in adhesive bonding has been demonstrated by the Authors in previous works. It has been shown that the impregnation method developed and refined during the previous studies allow generating high-quality pre-preg nanomats out of a 2k unfilled epoxy resin. By applying this methodology, in the present work, rubbery nanofibrous mats have been adopted for the first time to reinforce and increase the fracture toughness of adhesive joints. Rubbery nanofibers were produced by electrospinning of nitrile butadiene rubber (NBR) and poly(ε-caprolactone) (PCL). The addition of the semi-crystalline polymer (PCL) is exploited to maintain the nanofibrous morphology, which the rubber alone (NBR) would not be able to ensure due to its low glass transition temperature (Tg). The nanofibers thus obtained have been integrated into a two-component high strength epoxy resin for structural applications. S235 steel adherends for Double Cantilever Beam (DCB) tests have been manufactured and sandblasted to improve adhesion. An optimization of the sandblasting parameters (distance, pressure, angle and time) has been carried out evaluating the shear strength and the fracture surfaces on S235 steel Single Lap Joints (SLJ). Finally, DCB tests have been performed to compare the mode I fracture toughness with and without the rubbery electrospun nanomats.

Abstract Background Non-invasive delivery of nebulized surfactant has been a neonatology long-pursued goal. Nevertheless, the clinical efficacy of nebulized surfactant remains inconclusive, in part, due to the great technical challenges of depositing nebulized drugs in the lungs of preterm infants. The aim of this study was to investigate the feasibility of delivering nebulized surfactant (poractant alfa) in vitro and in vivo with an adapted, neonate-tailored aerosol delivery strategy. Methods Particle size distribution of undiluted poractant alfa aerosols generated by a customized eFlow-Neos nebulizer system was determined by laser diffraction. The theoretical nebulized surfactant lung dose was estimated in vitro in a clinical setting replica including a neonatal continuous positive airway pressure (CPAP) circuit, a cast of the upper airways of a preterm neonate, and a breath simulator programmed with the tidal breathing pattern of an infant with mild respiratory distress syndrome (RDS). A dose-response study with nebulized surfactant covering the 100–600 mg/kg nominal dose-range was conducted in RDS-modelling, lung-lavaged spontaneously-breathing rabbits managed with nasal CPAP. The effects of nebulized poractant alfa on arterial gas exchange and lung mechanics were assessed. Exogenous alveolar disaturated-phosphatidylcholine (DSPC) in the lungs was measured as a proxy of surfactant deposition efficacy. Results Laser diffraction studies demonstrated suitable aerosol characteristics for inhalation (mass median diameter, MMD = 3 μm). The mean surfactant lung dose determined in vitro was 13.7% ± 4.0 of the 200 mg/kg nominal dose. Nebulized surfactant delivered to spontaneously-breathing rabbits during nasal CPAP significantly improved arterial oxygenation compared to animals receiving CPAP only. Particularly, the groups of animals treated with 200 mg/kg and 400 mg/kg of nebulized poractant alfa achieved an equivalent pulmonary response in terms of oxygenation and lung mechanics as the group of animals treated with instilled surfactant (200 mg/kg). Conclusions The customized eFlow-Neos vibrating-membrane nebulizer system efficiently generated respirable aerosols of undiluted poractant alfa. Nebulized surfactant delivered at doses of 200 mg/kg and 400 mg/kg elicited a pulmonary response equivalent to that observed after treatment with an intratracheal surfactant bolus of 200 mg/kg. This bench-characterized nebulized surfactant delivery strategy is now under evaluation in Phase II clinical trial (EUDRACT No.:2016–004547-36).

This letter provides the first report of 160-Gb/s optical time-division-multiplexed transmission with all-channel independent modulation and all-channel simultaneous demultiplexing. By using a multiplexer and a demultiplexer based on periodically poled lithium niobate and semiconductor optical amplifier hybrid integrated planar lightwave circuits, 160-km transmission is successfully demonstrated. [ABSTRACT FROM PUBLISHER]

A novel mesophilic bacterium, strain SS33 T , was isolated from a deep-sea hydrothermal vent chimney at Suiyo Seamount, Izu-Bonin Arc, Western Pacific Ocean. The cells of strain SS33 T were motile short rods with a single polar flagellum. The growth of strain SS33 T was observed at the temperature range between 33 and 55 °C (optimum growth at 45 °C), at the pH range between 5.0 and 7.1 (optimum growth at pH 6.0) and in the presence of between 2.0 and 4.5% (w/v) NaCl [optimum growth at 3.5% (w/v)]. Strain SS33 T was a facultative anaerobic chemolithoautotroph using molecular hydrogen and elemental sulphur as the sole electron donor. Nitrate, nitrous oxide, sulphate, elemental sulphur and molecular oxygen were capable of serving as the sole electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences placed strain SS33 T in the genus Hydrogenimonas belonging to the class Epsilonproteobacteria . The closely related species of strain SS33 T were Hydrogenimonas urashimensis SSM-Sur55 T (95.96%), Hydrogenimonas thermophila EP1-55-1% T (95.75%) and Hydrogenimonas cancrithermarum ISO32 T (95.24%). According to the taxonomic and physiological characteristics, it is proposed that strain SS33 T was classified into a novel species of genus Hydrogenimonas , Hydrogenimonas leucolamina sp. nov., with SS33 T (=JCM 39184 T =KCTC 25253 T ) as the type strain. Furthermore, the genome comparison of Epsilonproteobacteria revealed that their [NiFe] hydrogenase genes belonging to Group 1b could be divided into two phylogenetic lineages and suggested that the reverse gyrase gene has been lost after division to the genus Hydrogenimonas .

Nitrosophilus labii HRV44 T is a thermophilic chemolithoautotroph possessing clade II type nitrous oxide (N 2 O) reductase (NosZ) that has an outstanding activity in reducing N 2 O to dinitrogen gas. Here, we attempt to understand molecular responses of HRV44 T to N 2 O. Time course transcriptome and proteomic mass spectrometry analyses under anaerobic conditions revealed that most of transcripts and peptides related to denitrification were constitutively detected, even in the absence of any nitrogen oxides as electron acceptors. Gene expressions involved in electron transport to NosZ were upregulated within 3 h in response to N 2 O, rather than upregulation of nos genes. Two genes encoding Crp/Fnr transcriptional regulators observed upstream of nap and nor gene clusters had significant negative correlations with nosZ expression. Statistical path analysis further inferred a significant causal relationship between the gene expression of nosZ and that of one Crp/Fnr regulators. Our findings contribute to understanding the transcriptional regulation in clade II type N 2 O-reducers., Competing Interests: The authors have no competing interests., (© 2024 The Author(s).)

Background: Histological mucosal healing has become a paramount target goal to achieve in the treatment of inflammatory bowel diseases. However, there is still a lack of agreement on the best way to reach this goal, since numerous histological scores are available worldwide., Aims: We investigated whether claudin-2, a member of claudin family involved in the regulation of intestinal tight junctions, might be useful to assess the presence of active disease in patients with inflammatory bowel diseases., Methods: Biopsies from 123 patients with ulcerative colitis, Crohn's disease, infectious colitides and irritable bowel syndrome patients where tested with immunohistochemistry for claudin-2., Results: Claudin-2 appeared to be a very sensitive marker of disease activity in inflammatory bowel diseases, but was negative in the other kinds of patients. In addition, immunohistochemistry for claudin-2 showed good reproducibility by different pathologists., Conclusions: Should these findings be confirmed in more numerous cohorts of patients, and especially in those with minimal or focal residual disease activity, this simple assessment could be useful in the routine daily practice to facilitate the task of pathologists and clinicians in the diagnosis and management of patients with inflammatory bowel diseases., Competing Interests: Conflict of interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Competing Interests: Conflict of interest All the authors declare no conflicts of interest.

Three novel bacterial strains, FE4 T , FE10 T , and LA51 T , which are phylogenetically affiliated to the genera Pseudoalteromonas, Vibrio, or Marinobacter, respectively, isolated from fertilized eggs and juveniles of sea cucumber Apostichopus japonicus were characterized by a genome-based taxonomical approach including multilocus sequence analysis (MLSA) combined with classical phenotypic and chemotaxonomic characterizations. A molecular network reconstructed on the basis of nucleotide sequences of four phylogenetic maker protein genes revealed that the strains FE4 T , FE10 T , and LA51 T were closely related to Pseudoalteromonas shioyasakiensis, Vibrio lentus, and Marinobacter similis, respectively. Average nucleotide identity (ANI) comparisons against phylogenetically related species to FE4 T , FE10 T , and LA51 T demonstrated that each newly described strain could not be identified as any previously described species within each genus showing < 95% ANI: 91.3% of FE4 T against P. shioyasakiensis JCM 18891  T , 92.6% of FE10 T against "V. bathopelagicus" Sal10, and 92.6% of LA51 T against M. similis A3d10 T , in maximum, respectively. Here, we show molecular phylogenetic, genomic, phenotypic, and chemotaxonomic features of the newly described species FE4 T , FE10 T , and LA51 T . We also propose Pseudoalteromonas apostichopi sp. nov. with FE4 T (JCM 36173  T  = LMG 33143  T ) as the type strain, Vibrio apostichopi sp. nov. with FE10 T (JCM 36174  T  = LMG 33144  T ) as the type strain, and Marinobacter apostichopi sp. nov. with LA51 T (JCM 36175  T  = LMG 33145  T ) as the type strain., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Pyruvate (Pyr) is the end product of the glycolysis pathway. Pyr is also renewable and is further metabolized to produce formate, which is the precursor of H 2 , via pyruvate formate lyase (PFL) under anaerobic conditions. The formate is excluded and re-imported via the formate channel and is then converted to H 2 via the formate hydrogenlyase (FHL) complex. In H 2 producing marine vibrios, such as Vibrio tritonius and Vibrio porteresiae in the Porteresiae clade of the family Vibrionaceae, apparent but inefficient H 2 production from Pyr has been observed. To elucidate the molecular mechanism of why this inefficient H 2 production is observed in Pry-metabolized marine vibrio cells and how glycolysis affects those H 2 productions of marine vibrios, the "Core Transcriptome" approach to find common gene expressions of those two major H 2 producing Vibrio species in Pyr metabolism was first applied. In the Pyr-metabolized vibrio cells, genes for the "Phosphoenolpyruvate (PEP)-Pyruvate-Oxalate (PPO)" node, due to energy saving, and PhoB-, RhaR-, and DeoR-regulons were regulated. Interestingly, a gene responsible for oxalate/formate family antiporter was up-regulated in Pyr-metabolized cells compared to those of Glc-metabolized cells, which provides new insights into the uses of alternative formate exclusion mechanics due to energy deficiencies in Pyr-metabolized marine vibrios cells. We further discuss the contribution of the Embden-Meyerhof-Parnas (EMP) pathway to efficient H 2 production in marine vibrios., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Here we provide the complete genome sequences of two chemoautotrophic isolates from the Thioglobaceae family of marine gamma-proteobacteria. The genomes were obtained from pure cultures that were initially isolated from Effingham Inlet in 2013 and revived from freezer stocks for whole genome sequencing in 2023., Competing Interests: The authors declare no conflict of interest.

Polybutylene succinate (PBS) is an eco-friendly green plastic. However, PBS was shown as being non-biodegradable in marine environments, and up until now, only a limited number of PBS-degrading marine microbes have been discovered. We first set up in vitro PBS- and PBSA (polybutylene succinate adipate)-plastispheres to characterize novel PBS-degrading marine microbes. Microbial growth and oxygen consumption were observed in both PBS- and PBSA-plastispheres enriched with natural seawater collected from Usujiri, Hokkaido, Japan, and Vibrionaceae and Pseudoalteromonadaceae were significantly enriched on these films. Further gene identification indicated that vibrios belonging to the Gazogenes clade possess genes related to a PBS degrading enzyme (PBSase). The PBS degradation assay for six Gazogenes clade vibrios identified Vibrio ruber, Vibrio rhizosphaerae, and Vibrio spartinae as being capable of degrading PBS. We further identified the gene responsible for PBSase from the type strain of V. ruber, and the purified recombinant vibrio PBSase was found to have low-temperature adaptation and was active under high NaCl concentrations. We also provided docking models between the vibrio PBSase and PBS and PBSA units to show how vibrio PBSase interacts with each substrate compared to the Acidovorax PBSase. These results could contribute to a more sustainable society through further utilization of PBS in marine environments and plastic recycling., (© 2023 Applied Microbiology International and John Wiley & Sons Ltd.)

In some sporting events (e.g., long-distance running), the ability to maintain joint stiffness is considered an essential physical ability. However, the determinants of joint stiffness endurance remain unclear. This study aimed to examine the effects of muscular endurance and tendon extensibility on joint stiffness endurance. Thirteen males performed the fatigue task (5 sets of 50 hopping). Ankle joint stiffness during drop jump was measured before and after fatigue task. The maximum number of repetitions at 30% of one repetition maximum for plantar flexion was measured as muscular endurance. Maximal elongation of the Achilles tendon was measured during ramp (with a low strain rate of tendon) and ballistic (with a high strain rate of tendon) contractions as tendon extensibility. Joint stiffness significantly decreased by 7.5% after the fatigue task ( p  = 0.033). The maximum number of repetitions at 30% of 1RM (79.6 ± 48.7 repetitions) was not significantly correlated with the relative change in joint stiffness ( r  = 0.283, p  = 0.348). The maximal elongation of the Achilles tendon measured during ramp and ballistic contractions were not significantly associated with the relative change in joint stiffness ( r  = 0.326, p  = 0.277 for ramp contraction; r  = 0.438, p  = 0.135 for ballistic contraction). These results suggest that muscular endurance and tendon extensibility were unrelated to joint stiffness endurance.