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Here, we present the draft genome assembly of Escherichia coli DBS1, which was originally isolated from a urine sample from a male patient with urinary tract infections in Rabat, Morocco.

PURPOSE Incidence of pediatric urolithiasis is decreasing in most developing countries where endemic bladder stones are less prevalent than in the past years. In parallel, stone composition has changed. Only few data are available in North Africa, except for Tunisia. We report stone composition in the Moroccan pediatric population. MATERIAL AND METHODS Composition of 432 stones from children (302 boys, 130 girls) was determined by infrared spectroscopy. The samples were collected during the period 1999-2016. Stone morphology, which is an important aspect for etiology was determined by examination of each stone under a stereomicroscope. Stone composition was compared to patients' age and gender. RESULTS The global male-to-female ratio was 2.32. Regarding stone composition, calcium oxalate was the main component in 51.6% of the stones, followed by struvite (18.1%), ammonium urate (9.5%) and carbapatite (9%). Significant differences were found between males and females: calcium oxalate accounted for 72.3% of stones in girls and 42.7% in boys (P

International audience; Staphylococcus capitis is a coagulase-negative staphylococcus (CoNS) commonly found in the human microflora. Recently, a clonal population of Staphylococcus capitis (denominated NRCS-A) was found to be a major cause of late-onset sepsis (LOS) in several neonatal intensive care units in France. Here, we report the complete genome sequence and annotation of the prototype Staphylococcus capitis NCRS-A strain CR01. The 2,504,472 bp long genome (1 chromosome and no plasmids) exhibits a G+C content of 32.81%, and contains 2,468 protein-coding and 59 tRNA genes and 4 rRNA genes.

Here, we report the draft genome sequences of methicillin-susceptible Staphylococcus captis pulsotype NCRS-C (CR02 strain) and multiresistant Staphylococcus captis pulsotype NCRS-A (CR07 strain).

Staphylococcus capitis pulsotype NRCS-A was previously reported as a frequent cause of late-onset sepsis in neonatal intensive care units (NICUs) worldwide. Here, we report the whole-genome shotgun sequences of four S. capitis pulsotype NCRS-A strains, CR03, CR04, CR05, and CR09, isolated from Belgium, Australia, the United Kingdom, and France, respectively., SCOPUS: ar.j, info:eu-repo/semantics/published

cystine stones represent 1% of urinary calculi in adults and 10% in children and are especially recurrent and resistant to ESWL. Medical therapy is often efficient but often poorly tolerated. In Morocco, various plants, Herniaria hirsuta, Opuntia ficus-indica, Zea mays and Ammi visnaga are proposed against nephrolithiasis. We assessed the effect of plant extracts on the disolution of cystine stones in vitro.an extract of each plant was prepared by infusion of two grams of powdered plants during 30 minutes in 100ml of a boiled NaCl 9 g/L aqueous solution. Each extract was then filtered and thereafter set in a flask containing a cystine stone. The medium was maintained under stirring during 8 weeks. NaCl 9 g/L solution and sodium citrate 3 mmol/L solution were used as controls. At the end of each two weeks period, the stone was removed from the experimental medium and weighted after a 16h drying period at 40°C.after 8 weeks of experiment, stone dissolution was complete for all herbal extracts, and was earlier in the presence of Zea mays (4 weeks) or of Ammi visnaga (6 weeks) by comparison to only 18 ± 8 and 20 ± 1.5 % for citrate and NaCl solutions, respectively.the studied herbal extracts were efficient for dissolving cystine stones, probably resulting from the formation of complexes between cystine and polyhydroxylated molecules present in the extracts. These results, to be confirmed in vivo, underline the potential interest of the plant extracts to treat cystine stones.

Objectives We aimed to analyze in children suffering from chronic haemophilic arthropathy relation between there functional impact and there quality of life. Methods Twenty- three children (mean age of 10.9±3.5 years [4-18]) suffering from severe haemophilia (median disease duration of 12 months (6; 18),median diagnosis delay of 0 months (0, 7)) were included. Functional impairment and quality of life were respectively assessed by the Moroccan versions of Children Health Assessment Questionnaire (CHAQ) and the European Quality of Life (the EUROQOL). A statistical analysis was conducted to determine the correlation between these two parameters using the r of Spearman Results Chronic haemophilic arthropathy affected knee, elbow, ankle and wrist in respectively 50, 27.5, 20 and 2.5%. Median CHAQ was 0.37 (0, 1.25). EUROQOL domains were affected as following: Mobility (69.6%),usual activity (65.2%), self-care (65.2%), anxiety/depression (43.4%) and pain (34.7%) Table 1 summarize correlation between the CHAQ and the 5 domains of EUROQOL. Conclusions This study suggests that the functional impact of chronic haemophilic arthropathy in children affected by severe hemophilia seems mainly related to the domains of mobility, self-care and usual activity of the EUROQOL. Further studies are required to verify those results. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.4867

Background Research on patients with chronic illness has demonstrated that self-esteem is related to the reported level of physical restrictions, functional status and perceiption of symptom severity. Previous literature suggests that low self-esteem causes more negative affect for chronic disease patients than healthy populations. Objectives This study aims to evaluate the frequency of low self esteem in patients with rheumatoid arthritis (RA) and to identify factors influencing it. Methods It is a cross-sectional study including 103 patients with RA. The demographic characteristics of patients and the characteristics of RA were collected. Pain and fatigue were assessed using the analogic visual scale (0-100 mm), the disease's activity was evaluated by DAS-28 ESR, the functionnal disability by the arabic version of the health assessment questionnaire (HAQ), and the the psychological state by the arabic version of the questionnaire Hospital Anxiety and Depression (HAD). The level of self-esteem was measured using the Rosenberg Self-esteem Scale (RSES). Participants have reported their agreement, on a five-point Likert scale (0 = strongly disagree, 4 = strongly agree) on 10 statements concerning self-perceived aspects of self-esteem. Total scores range from 10-40. A low self esteem rate was defined by a general scroe less than 311. Results 103 patients were included with a mean age of 49.7±11.4 years and a female predominance (90 (87.4%)). The median of RA duration was 8.16 years [3.25 to 14.16]. The average DAS28 - ESR was 4.28±1.75, with a median HAQ 0.5 [0 -1.37]. 58 patients (56.3%) have low self esteem. Low self-esteem was significantly associated to a high activity of the RA (OR=1.652, IC95% [1.254-2.176], p

Objectives There have been few studies on the impact of rheumatoid arthritis (RA) on the work of the household. Our study aims to evaluate the impact of rheumatoid arthritis (RA) on women in Moroccan home. Methods Demographic data, clinical and biological characteristics of the disease were collected. Depression was assessed by HAD questionnaire (Hospital Anxiety and Depression Scale). Working from home has been assessed by a multiple choice questionnaire [1] (no problems, some problems, many difficulties and disabilities) on the following items: the ability to do household chores, cleaning the house, washing floor, washing the dishes, cooking, and caring for children and husband. Women who failed to achieve at least one household activity were considered unable to domestic work and they were compared to those who have no such disability. Univariate and multivariate logistic regression was performed to identify factors associated with this disability for domestic work. Results 90 women (mean age 49.7±11.4 years) with Rheumatoid arthritis were included. The disease9s median duration was 8 years [4,14], the median of DAS28-ESR was 4.27±1.75 and median of HAQ was 0.5 [0, 1, 37]. The inability to domestic work was found in 31 patients (34.5%), this inability related to the following items: do household activities (23.3%), clean the house (26.7%), wash the floor (34.4%), caring for children and husband (14.4%), washing dishes (26.7%) and cook ( 17.8%). In univariate analysis the inability of domestic work was associated with high disease activity (OR =2.23, p Conclusions We conclude that RA is highly predictive of homemaking disability in moroccan women, especially those with active RA and significant functional impairment. References Household work disability of Arab houseswives with rheumatoid arthritis. Clin Rheumatol. 2007. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.5812

Objectives Score distributions and associations between the RAID score and RA demographic and disease related variables were examined in rheumatoid arthritis (RA) patients. Methods 103 patients diagnosed were enrolled in the study. Disease activity was assessed through the Disease Activity Score (DAS) 28 scale. quality of life was evaluated with The EQ-5D, and Health Assessment Questionnaire (HAQ) was completed by all patients. Pain and fatigue were evaluated with pain Visual analogic scale (VAS) and fatigue VAS respectively. The Rheumatoid Arthritis Impact disease (RAID) was used for the evaluation of the impact of rheumatoid arthritis. Results The mean RAID was 3,78±2,15. The distribution of the RAID: 32% had a score between 0 and 2, and 13.5% between 7 and 10. In univariate analysis the RAID score correlated with pain (r=0,79; IC95% (0,05-0,06); p Conclusions This study suggested that RAID associated with disease activity, functional capacity, Pain, fatigue and EQ-5D. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.4863

Objectives This study aimed to evaluate the frequency of sleep disorders in patients with rheumatoid arthritis (RA) and to explore the determinants of these disorders. Methods It is a cross-sectional study including patients with RA. Patients with a known psychiatric disorder were excluded from the study. The demographic characteristics of patients and the characteristics of RA were collected. Pain and fatigue were assessed by a visual analogue scale (0-100 mm), the disease activity by DAS28 - ESR (Disease Activity Score), the Functional Disability by the Arabic validated version of the Health Assessment Questionnaire (HAQ), the quality of life by Euroqol 5D and psychological state by the Arabic validated version of the questionnaire Hospital Anxiety and Depression (HAD) with its two items anxiety and depression. Participants completed the self-rated questionnaire “Pittsburgh Sleep Quality Index (PSQI)” that assesses sleep quality and disturbances over a 1-month time interval. Nineteen individual items generate seven “component” scores: subjective sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbances, use of sleeping medication, and daytime dysfunction. The total score ranges from 0 (no disorder) and 21 (major problems) 1 . Univariate and multivariate linear regression was performed to determine factors associated with poor sleep quality. Results 103 patients were included with a mean age of 49.7±11.4 years and a female predominance (90 (87.4%)). The median of RA duration was 8.16 years [3.25 to 14.16]. The median of global score PSQI was 5 [2-9]. Poorer Sleep quality was significantly associated with high disease activity (r =0.381, 95% CI [0.465, 1.320], p Conclusions Our study suggests that sleep disorders, in rheumatoid arthritis, are more frequently found in patients with associated anxiety disorders. References Sleep Quality and Functional Disability in Patients with Rheumatoid Arthritis; FS Luyster, ER Chasens, MCM Wasko et al; Journal of Clinical Sleep Medicine, Vol. 7, No. 1, 2011 Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.4772

Objectives The purpose of this study was to identify physical activity behaviors of Moroccan patients with RA and her associations with RA demographic and disease related variables. Methods A cross sectional study was conducted in 103 RA. Socio-demographic characteristics and Disease specific variables were documented. Disease activity was measured with Disease Activity Score 28 (DAS28), quality of life was assessed with rheumatoid arthritis impact disease (RAID), fatigue was evaluated by fatigue Visual Analogic scale and Health Assessment Questionnaire (HAQ) was completed by all patients. Physical activity was evaluated with the Arab version of the International Physical Activity Questionnaire (IPAQ) short version. Results A total of 90 women and 13 men with RA participated in this study. Mean age of participants was 49.7±11.4.disease duration was a median of 8.16 years [3,25-14,16]. Total amount of physical activity was a median of 1873 (346-4053) MET-minute/week. However in 30.8% the physical activity was low. In univariate analysis lower physical activity (IPAQ) correlated with higher disease activity (OR=-3.9; CI95%>899–300; p 2146–845; p= 43.4–3.3; p=0,02) and low quality of life (RAID) (OR=-2.8; CI95%>610–107p=0,006). No relationship was found between PA, age, disease duration and BMI. After multivariate analysis HAQ (OR=-3.1; CI95%>991–85; p=0.002) and DAS28 (OR=-3.1;CI95%>2587–606) still strongly associated with PA. Conclusions This study suggested that lower physical activity associated with high disease activity and poor functional capacity. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.5826

Objectives To compare the disease activity score (DAS)28- ESR versus DAS28 -CRP, and to determine the factors that might influence their difference. Methods Patients with RA were included in a cross-sectional study. We have collected the demographic characteristics and the characteristics of the RA: duration of evolution, morning stiffness in minutes, functional impact of the disease assessed by the HAQ (Health Assessment Questionnaire), and current corticosteroid dose. The disease activity was assessed by the DAS28-ESR and DAS28-CRP. A simple correlation between DAS28-ESR and DAS28-CRP was performed. We considered a difference between DAS28-ESR and DAS28-CRP as significant when it is greater than or equal to 0.6 [1]. Factors influencing this difference were tested by univariate then multivariate logistic regression. Results 103 patients were included with a female predominance (87.4%). Mean age was 49.7±11.4 years. Median disease duration was 8 years [3-14]. There was a strong positive correlation between the two indexes of 0.91 (p Conclusions Our study shows a strong positive correlation between the DAS28-ESR and DAS28 -CRP. But DAS VS 28 would be higher than the DAS28 CRP in patients with high pain intensity and significant functional impairment. References Are the C-reactive protein values and erythrocyte sedimentation rate equivalent when estimating the 28-joint disease activity score in rheumatoid arthritis? Clin Exp Rheumatol. 2008 Sep-Oct;26(5):769-75. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.5807

Objectives The psychological aspect of rheumatoid arthritis (RA) is often overlooked in the clinical monitoring of the disease. The aim of our study was to determine the prevalence of depression and anxiety in rheumatoid arthritis (RA) and to identify factors that influence them. Methods Patients with RA were included in a cross-sectional study. We have collected the demographic characteristics and the characteristics of the RA: duration of evolution, morning stiffness in minutes, functional impact of the disease assessed by the HAQ (Health Assessment Questionnaire) and disease activity estimated by the DAS28-ESR. The psychiatric assessment was made by the DASS -21 (Depression, Anxiety and Stress Scale). Factors influencing depression and anxiety were tested by univariate and multivariate logistic regression. Results 103 patients were included with a mean age of 49.7±11.4 years and a female predominance (n=90 (87.4%)). the median of disease progression duration was 8.16 years [3.25-14.16]. The diagnosis of depression was found in 23.3% of patients and the anxiety in 30.1% of them. In the univariate analysis, depression and anxiety were associated with a high disease activity, a significant pain intensity and a significant functional impairment (p Conclusions Our study shows a high frequency of both anxiety and depression in the RA. It seems that anxiety is more frequent than depression. Both events would be more marked in patients with significant functional impairment. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.5817

Objectives Assessment of fatigue in Moroccan patients with rheumatoid arthritis (RA) and its relationship with disease parameters. Methods 1089 Moroccan patients with rheumatoid Arthritis (RA) were included in the multicenter cross-sectional study QUEST-RA (Quantitative Patient Questionnaires in Standard Monitoring of Patients with Rheumatoid Arthritis). The presence of fatigue was researched and its degree was assessed by a visual analogue scale (VAS) ranging from 0 to 100 mm. The presence and intensity of fatigue were correlated to the RA patient and disease parameters (sex, DAS 28, score of Health assessment Questionnaire (HAQ), corticosteroids intake, presence or absence of sleep disorders or psychological problems). Results The mean age of our patients was 49 ± 12.6 years with a female predominance of 80.3%. The average of disease duration was 6 (3, 12) years. The mean DAS28 was 4.9 ± 1.68. The average of HAQ was 1 (0.4, 1.6). Fatigue was present in 97.1% patients and the average of VAS fatigue was 43.2 ± 26.6 mm. A high level of fatigue was associated with female gender (44% versus 39%, p = 0.05), an important level of disease activity (r = 0.544, p Conclusions Fatigue is a major problem in our patients with RA and must be included in their routine assessment. In our sample, fatigue appears to be related to female sex, high level of disease activity and greater functional disability. Disclosure of Interest None Declared

Background Regular physical activity is associated with decreased morbidity and mortality. Traditionally, patients with rheumatoid arthritis (RA) have been advised to limit physical exercise. Objectives to Assess the impact of rheumatoid arthritis on physical activity. Methods The Questionnaires in Standard Monitoring of Patients with Rheumatoid Arthritis (QUEST-RA) is a cross-sectional study that includes a self-report questionnaire and clinical assessment of non selected consecutive outpatients with RA who are receiving usual clinical care. Frequency of physical exercise (>or=30 minutes with at least some shortness of breath, sweating) is queried with 4 response options: >or=3 times weekly, 1-2 times weekly, 1-2 times monthly, and no exercise. Results The total of 1129 patients were enrolled: 87,3% were women, the mean age was 48 years, and the mean disease duration was 6 years. Only 2,8% of all patients reported physical exercise>or=3 times weekly. The majority of the patients were physically inactive with no regular weekly exercise: >80. Physical inactivity was associated with disease duration, higher levels of disease activity, pain, morning stiffness and fatigue. Conclusions This study provided the evidence that the physical inactivity rates remain high among people with RA during this millennium. The rheumatologist should motivate patients to increase physical activity levels. Disclosure of Interest None Declared

Background Counter to the course of physical function and structural damage, few studies have addressed psychological health status as the primary outcomes in rheumatoid arthritis (RA) researches. Depression and its predictors among RA patients appear to be unrecognized or under-treated. In African and Arabic region, there are limited data outlining depression in RA. Objectives We aimed to determine the prevalence of depression in Moroccan patients with RA and to evaluate the disease-related parameters influencing it. Methods 250 consecutive patients with RA were recruited. Patients with previous psychological disorders and significant comorbidities were excluded. The Patient Health Questionnaire depression scale (PHQ-9) was used to measure depressive symptoms. For all patients were collected: socio-demographic characteristics; disease duration; joint pain intensity (visual analogue scale); disease activity (measured clinically using physical examination, biologically and by the disease activity scores (DAS28)); structural damage (by radiographs scored using the Sharp’s method as modified by Van der Heijde); functional disability (by the Health Assessment Questionnaire (HAQ)); extra-articular manifestations; immunological status and treatments. Fatigue was assessed on a 0-100 visual analogue scale (VAS fatigue) and the Arabic version of the generic instrument SF-36 was applied to assess quality of life (QoL). Results There was high prevalence of depressive syndrome among or RA patients: 164 (65.6%) patients have major depressive syndrome and 64 (25.6%) have minor depressive syndrome with a mean total score of PHQ-9 =17.3±8.1indicating a moderate-severe depression. Severe depressive syndrome and higher scores of PHQ-9 were associated with female gender, disease duration, pain intensity, clinical and biological disease activity (DAS28, ESR and CRP), functional disability, structural damage, high levels of RF and ACPAs, higher VAS fatigue scores and impaired physical and mental domains of QoL (for all p≤0.01). Also, patients with decreased levels of education and low socioeconomic status had significantly higher scores of depression (for all p Conclusions Depression is a major issue among our RA which influenced negatively different aspects of QoL. In our data, depression was associated with low socioeconomic and educational status, pain intensity, disease activity, fatigue, functional disability, ACPAs and structural damage. Depression must be recognized and included in the assessment and management of our patients. The advent of new treatments may improve depressive symptoms through their action on inflammation, pain, fatigue and physical disability. Disclosure of Interest None Declared

Depending on the basis of the technique used, an estimation of 2 to 6 lecithin molecules are required for reactivation of purified D-beta-hydroxybutyrate apodehydrogenase (apoBDH). 60 to 70 mitochondrial phospholipid molecules are necessary for insertion of one BDH polypeptide chain where 16 molecules would be in direct interaction with the enzyme. Results show off the cofactor role of lecithin and also the requirement of an amphiphilic environment, essential for the enzyme function.