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1. Acute, Sub-Acute and Cell Toxicity of Verbascoside

Author

Nasser Vahdati-Mashhadian, Reza Zafari, S. Adel Moallem, Zahra Oskouei Shirvan, Hossein Hosseinzadeh, and Leila Etemad

Subjects
chemistry.chemical_classification, business.industry, medicine.medical_treatment, Intraperitoneal injection, Glycoside, Phenylethanoid, Pharmacology, Median lethal dose, chemistry.chemical_compound, Verbascoside, Complementary and alternative medicine, chemistry, Toxicity, medicine, MTT assay, business, Cytotoxicity
Abstract

Verbascoside or acteoside is the most abundant phenylethanoid glycoside that possesses health beneficial pharmacological activities, including anti-nociceptive, anti-inflammatory and anti-cancer. Due to the wide range of pharmacological activities of verbascoside and insufficient data on the safety profile, the acute, subacute and cellular toxicity of verbascoside was determined. The acute and subacute toxicity of verbascoside was evaluated in mice after single intraperitoneal injection at the dose range of 0, 1, 2 and 5 g kg-1b.wt. (acute model) and 21 days administration at the dose range of 0, 10, 30 and 60 mg kg-1b.wt. (subacute model). In MTT assay, HepG2 and NIH cells were exposed to different concentrations of verbascoside. According to result, the LD 50 value of verbascoside was found to be greater than 5 g kg-1. In subacute toxicity study, no statistically significant differences were observed in the values of hematological, biochemical and pathological parameters in comparison with control group. The cytotoxicity assay revealed that the viability in all groups were greater than the IC 50 value. In conclusion, the results from the present study elucidate that verbascoside is well tolerated for both single and chronic administration and does not produce any toxic effects or deaths in animals. © 2015 Academic Journals Inc.

Published
2015
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2. Transglutaminase and Clusterin Induction during Normal and Abnormal Limb Development in the Mouse1

Author

Barbara F. Hales and S. Adel Moallem

Subjects
Programmed cell death, Clusterin, Ratón, Tissue transglutaminase, Cell Biology, General Medicine, Immunogold labelling, Biology, Molecular biology, Reproductive Medicine, Apoptosis, Immunology, biology.protein, Immunohistochemistry, Limb development
Abstract

Apoptotic cell death is important in pattern formation in the limb. The purpose of this study was to investigate the relationship between the occurrence of apoptosis in the mouse limb during normal and abnormal development as well as the expression of tissue transglutaminase and clusterin, two proteins associated with apoptotic cell death. Mouse limb buds were cultured in vitro in the absence or presence of a potent teratogen, an activated analog of cyclophosphamide: 4-hydroperoxycyclophosphamide (1 or 10 micrograms/ml). Using whole-mount in situ DNA labeling and confocal microscopy, apoptotic cells were localized in the interdigital areas of control limbs after culture for 24 h. The number of apoptotic cells in the interdigital areas of the limbs was increased in the presence of 4-hydroperoxycyclophosphamide (1 microgram/ml). Exposure to a higher concentration (10 micrograms/ml) of 4-hydroperoxycyclophosphamide further increased the numbers of cells staining positively for apoptosis. The relative abundance of tissue transglutaminase increased 3-4-fold after 6 or 24 h of culture with either concentration of 4-hydroperoxycyclophosphamide; immunoreactive protein in drug-treated limbs decreased to control levels by 48 h. Transglutaminase immunoreactivity was localized in the interdigital areas of limbs 24 h after drug exposure. Clusterin immunoreactivity in the control limbs was weak. The abundance of clusterin was increased 3-4-fold in drug-treated limbs; this induction occurred only after 48 h of culture with 4-hydroperoxycyclophosphamide. Clusterin immunoreactivity in limbs after drug treatment for 48 h was localized to the interdigital areas; immunogold electron microscopy of clusterin expression showed a specific labeling in phagocytosed apoptotic bodies. Thus, the number of cells staining positively for apoptosis in the limb was greatly increased in the interdigital areas during abnormal limb development. The expression of both transglutaminase and clusterin was altered in areas of the limb undergoing apoptosis during abnormal limb development.

Published
1996
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3. Poster presentations

Author

Funda Aksu, Hakan Topacoglu, Candan Arman, Aytul Atac, Suleyman Tetik, Aida Hasanovic, Amela Kulenovic, Zakira Mornjakovic, Branko Pikula, Aida Sarac-Hadzihalilovic, Alma Voljevica, Belgin Bamac, Tuncay Colak, Murat Alemdar, Gulmine Dundar, Macit Selekler, Ozgur Dincer, Enis Colak, Aydin Ozbek, Cenk Kilic, Kivanc Kamburoglu, Tuncer Ozen, Vatan Kavak, Yalcin Kirici, Emin Oztas, Handan Altinkaya Soysal, Erdogan Unur, Nihat Ekinci, Omur Karaca, Olga Malakhova, Murat Kocaoglu, Serdar Toker, Figen Taser, Volkan Kilincoglu, Mustafa Fahri Yurtgun, Cannur Dalcik, Ali Zeybek, Marc Baroncini, Johann Peltier, Patrice Jissendi, Jean-Pierre Pruvo, Jean-Paul Francke, Vincent Prevot, Rengin Kosif, Yasin Arifoglu, Murat Diramali, Mustafa Sarsilmaz, Evren Kose, Murat Ogeturk, Burhan Akpinar, Ilter Kus, Sedat Meydan, Alev Kara, Zeliha Kurtoglu, Ibrahim Tekdemir, Alaittin Elhan, Orhan Bas, Ersan Odaci, Hakan Mollaoglu, Kagan Ucok, Suleyman Kaplan, Mehmet Senoglu, Vedat Nacitarhan, Ergul Belge Kurutas, Nimet Senoglu, Idris Altun, Yalcin Atli, Davut Ozbag, Sacide Karakas, M. Dincer Bilgin, Ayfer Metin Tellioglu, Sercin Ozlem, Betul Akcanal, Yuksel Yildiz, Hakki Gunes, Hayrullah Kose, Ibrahim Uzum, Umit Naci Gundogmus, Cigdem Caglayan, Velichka Pavlova, Mashenka Dimitrova, Lilia Georgieva, Elena Nikolova, Deniz Uzmansel, Nail Can Ozturk, Canan Yurttas Saylam, Erkin Ozgiray, Mustafa Orhan, Sedat Cagli, Mehmet Zileli, Derya Ozkan, Taylan Akkaya, Ayhan Comert, Nilgun Balikci, Esra Ozdemir, Haluk Gumus, Zafer Ergul, Oskay Kaya, Serdar Altun, R. Erkin Unlu, Hakan Orbay, Deog-Im Kim, Seung-Ho Han, Yi-Suk Kim, Ho-Jeong Kim, Kyu-Seok Lee, Omur Elcioglu, Hilmi Ozden, Gul Guven, Nurcan Imre, Bulent Yalcin, Hasan Ozan, Pinar Akyer, Mustafa Guvencer, Vasfi Karatosun, Mandeep Gill Sagoo, Rachel Claire Aland, Derya Ustuner, M. Cengiz Ustuner, Jafar Ai, Seyed Reza Ghazi, Seyed Hadi Mansouri, Mehmet Cudi Tuncer, Mehmet Ufuk Aluclu, Ozlen Karabulut, Eyup Savas Hatipoglu, Hasan Nazaroglu, Cigdem Icke, Emrah Akbay, Turkan Gunay, Suleyman Icke, Selda Yildiz, Fatih Yazar, Barcin Orhan Barlas, Delia Elena Zahoi, Ahmet Kavakli, Ufuk Tas, Durrin Ozlem Dabak, Hilal Irmak Sapmaz, Necdet Kocabiyik, Cenk Murat Ozer, Ayhan Ozcan, Levent Elevli, Kadir Desdicioglu, Ibrahim Alanbay, Figen Govsa, Canan Y. Saylam, Ilgaz Akdogan, Yilmaz Kiroglu, Sule Onur, Emine Hilal Evcil, Neslihan Cankara, Mehmet Ali Malas, M. Tayyar Kalcioglu, Serdar Duman, Tufan Ulcay, Ahmet Uzun, Zulfu Karabulut, Cagatay Barut, Ozdemir Sevinc, Gamze Yurdakan, Dundar Kacar, Ali Riza Erdogan, Hulyam Kurt, Bunyamin Demir, Mustafa Saltan, Dilek Burukoglu, Mehmet Cengiz Ustuner, Irfan Degirmenci, Aliriza Erdogan, Ozlem Damar, Merih Is, Gokhan Bayramoglu, Sahin Kabay, Onur Uysal, Hakan Senturk, Aysegul Bayramoglu, Cansu Ozbayar, Ali Kutlu, Mediha Canbek, Salih Cenap Cevli, Oguz Hancerlioglu, Mustafa Koplay, Elif Aksakalli, Fatih Dikici, Aysin Kale, Ozcan Gayretli, Ilke Ali Gurses, Senem Turan Ozdemir, Ilker Ercan, Emel Bulbul Baskan, Mediha Yilmaz, Guven Ozkaya, Hayriye Saricaoglu, Mete Erturk, Gulgun Kayalioglu, Mehmet Uzel, Guler Kahraman, Ercan Tanyeli, Ali Ihsan Soyluoglu, Orhan Tacar, Ayda Demirant, Murat Bilgin, Aziz Karadede, Ayfer Aktas, E. Hilal Evcil, Esra Koyuncu, Osman Sulak, Soner Albay, Gulnur Ozguner, Ahmet Ozbek, Elvan Ozbek, A. Hakan Ozturk, Tuba Demirci, Engin Ciftcioglu, Mehmet Tevfik Demir, Cem Kopuz, Esra Eroglu, Semin Gedikli, Hamit Ozyurek, Mehmet Selim Nural, Lutfi Incesu, Gonul Ogur, Engin Kara, Baris Celebi, Altan Yildiz, B. Zuhal Altunkaynak, Samet Vasfi Kuvat, Suleyman Murat Tagil, Cumhur Ertekin, Hilmi Uysal, Fikret Bademkiran, Nural Albayrak, Ali Firat Esmer, Nigar Keles Coskun, Muzaffer Sindel, Ferah Kizilay, Sevket Yalin, Nevin Karapinar, Mehmet Tokdemir, Lokman Karakurt, Levent Tumkaya, Adnan Korkmaz, Bulent Ayas, Nusret Ciftci, Yuksel Terzi, Ozlem Baran, Yusuf Nergiz, Murat Akkus, Ufuk Aluclu, Askin Ender Topal, Dilek Yuksel, Halil Ibrahim Acar, Simel Kendir, Emre Hekimoglu, Deniz Basman, Sunay Duman, Baris Ozener, Can Pelin, Ragiba Zagyapan, Ayla Kurkcuoglu, Mustafa Koc, Meral Erdinc, Levent Erdinc, Ilker Kelle, Enver Sancakdar, Nil Cetin, Selcuk Tunik, Ayse Yildirim, Iskender Kaplanoglu, Ercan Ayaz, Necip Ilhan, Mehmet Okumus, Kasim Zafer Yuksel, Harun Ciralik, Zeki Yilmaz, Yakup Gumusalan, Mehmet Gamsizkan, Mustafa Kazkayasi, Nadire Unver Dogan, Ismihan Ilknur Uysal, Aylin Karalezli, Zeliha Fazliogullari, Mustafa Buyukmumcu, Mehmet Cem Bozkurt, Aynur Emine Cicekcibasi, Deniz Demiryurek, M. Hakan Ozsoy, Alp Bayramoglu, Eray Tuccar, Ozlem Pamukcu Baran, Sevda Soker, Selen Bahceci, Yasemin Nasir, Mehmet Tugrul Yilmaz, Emine Aynur Cicekcibasi, Mahinur Ulusoy, Pervin Gunaslan, Nuray Bilge, Muzaffer Akkaya, Abdurrahman Genc, Sezer Akcer, Yucel Gonul, Emine Cosar, Gulengul Koken, Ilknur Ari, Sinan Bakirci, Ilker Mustafa Kafa, Murat Uysal, Ahmet Kagan Karabulut, Bahar Keles, Dilek Emlik, Yavuz Uyar, Kayhan Ozturk, Neslihan Altuntas Yilmaz, Ahmet Salbacak, Burkay Kutluhan Kacira, Mehmet Arazi, Serafettin Demirci, Demet Kiresi, Serter Gumus, Muzaffer Seker, Mehmet Uyar, Mohammad Ebrahim Astaneh, Alireza Khorshid, Ramazan Uygur, Ahmet Songur, Osman Fikret Sonmez, Kamil Hakan Dogan, Giray Kolcu, Madalina Iliescu, Petru Bordei, Dan Iliescu, Camelia Ciobotaru, Viorel Lucescu, Anatoli Covaleov, Constantin Ionescu, Miguel Guirao, E. Páramo, R. Mutuberria, I. Sánchez-Montesinos, O. Roda, F. Girón, Miguel Lopez-Soler, Olga Roda, Raúl Campos-López, Miguel Guirao-Piñeiro, Maria Teresa Pascual-Morenilla, Indalecio Sanchez-Montesinos, Maria Teresa Pascual, I. Garzon, D. Serrato, R. Nieto-Aguilar, I. Sanchez-Montesinos, M. Sanchez-Quevedo, M. Bulent Ozdemir, R. Hakan Ozean, Dilek Bagdatli, Esat Adiguzel, Zumrut Dogan, Ozlem Aycan, Nigar Vardi, Haldun Sukru Erkal, Hakan Ozturk, S. Mocanu, C. Stefanescu, A. Ionescu, Raluca Talpes, Elena Sapte, Constantin Dina, Loredana Surdu, Ionut Bulbuc, M. T. Medina, J. Medina, M. López-Soler, Carlos Martin-Oviedo, Alejandro Lowy-Benoliel, Eva Maranillo, Tomas Martinez-Guirado, Jose Sañudo, Bartolome Scola, Teresa Vazquez, L. A. Arráez-Aybar, J. L. Conejo-Menor, C. C. Gonzáles-Gómez, A. J. Torres-García, Hisayo Nasu, Shoji Chiba, M. Gutierrez-Semillera, Yahya Paksoy, Ahmet Kalaycioglu, Mehmet Yildirim, Ali Ozyasar, Omer Ozdogmus, Yusuf Ozgur Cakmak, Ural Verimli, Safiye Cavdar, Begum Yildizhan, Z. Asli Aktan Ikiz, Hulya Ucerler, Zuhal Ozgur, Seher Yilmaz, Abdullah Demirtas, Ertugrul Mavili, Mehtap Hacialiogullari, Hatice Susar, Seda Arslan, Kenan Aycan, Vecihi Ozkaya, Mara Pilmane, Sarmite Boka, Gursel Ortug, Carlos Ramirez, Aran Pascual-Font, Francisco Valderrama-Canales, Abdulah Kucukalic, Eldan Kapur, Elvira Talovic, Vaclav Baca, Robert Grill, Zdenek Horak, David Kachlik, Valer Dzupa, Marek Konarik, Jakub Knize, Petr Veleminsky, Tereza Smrzova, Michal Otcenasek, Jana Chmelova, Michal Kheck, Tomas Cupka, Lukas Hnatek, Floris van der Meijs, Pavel Cech, Vladimir Musil, H. Mustafa Ozkan, S. Kivanc Muratli, Hamid Tayefi, Ipek Ergur, Amac Kiray, Muhsin Toktas, Ozan Alkoc, Tolgahan Acar, Ibrahim Uzun, Oguz Asian Ozen, Abdullah Aycicek, Ozan Alper Alkoc, Mehmet Unlu, Ufuk Corumlu, Ihsaniye Coskun Ikiz, I. Hakan Oygucu, Erdogan Sendemir, Tuncay Kaner, Veli Caglar, Olcay Eser, Mehmet T. Demir, Omer Iyigun, Gokhan Pirzirenli, Ahmet Hilmi Kaya, Mennan Ece Aydin, Fahrettin Celik, Hakan True, Sevket Ozkaya, Bekir Ugur Ergur, Gulsah Zeybek, Kadir Bacakoglu, Mina Tadjalli, Aghdas Poostpasand, Seid Hadi Mansouiri, Ozra Allahvaisi, Jafar Soleimanirad, Bahram Nikkhoo, Yasukazu Nagato, Yasuo Haruki, Komazo Yazawa, Tutomu Okazaki, Munetaka Haida, Yutaka Imai, Thmineh Peirouvi, Mehrzad Mahzad-Sadaghiani, Farahnaz Noroozinia, Salami Siamak, Gholamhosseine Farjah, Sima Mola, Ewa Biegaj, Tymon Skadorwa, Konrad Pawlewicz, Robert Kapolka, Agata Chachulska, Joanna Zabicka, Aleksandra Krasowska, Alicja Prusik, Grzegorz Jaczewski, Adam Kolesnik, M. Mohsen Taghavi, S. Hasan Alavi, S. Adel Moallem, Zahed Safikhani, Marzieh Panahi, Shahriar Dabiri, Majid Asadi Shekaari, Rafael Latorre, Federico Soria, Octavio Lopez-Albors, Ricardo Sarria, Inacio Ayala, Inma Serrano, Enrique Perez-Cuadrado, Vladimir Musienko, Dmitry Tkachenko, Neriman Colakoglu, Murat Abdulgani Kus, Mahdi Jalali, Mohammad Reza Nikravesh, Abbas Ali Moeen, Mohammad Hassan Karimfar, Houshang Rafighdoost, Shabnam Mohammadi, Marina Korneeva, Houshang Rafighdoust, Kvetuse Lovasova, Adriana Bolekova, Darina Kluchova, Igor Sulla, Marina Yurievna Kapitonova, Syed Baharom Syed Ahmad Fuad, Flossie Jayakaran, Ali Reza Shams, Fereshteh Aghaee, Zohreh Baqer, Mohamad Faroki, Srijit Das, Normadiah Kassim, Azian Latiff, Frihah Suhaimi, Norzana Ghafar, Khin Pa Pa Hlaing, Israa Maatoq, Faizah Othman, Muge Kiray, Husnu Alper Bagriyanik, Cetin Pekcetin, Candan Ozogul, Mustafa Fidan, Farihah Suhaimi, Fei Sun, Francisco Sanchez-Margallo, Francisco Gil, Verónica Crisostomo, Jesus Uson, Gegorio Ramirez, Ozan Turamanlar, Oguz Kirpiko, Alpay Haktanir, Salvador Climent, Sergio Losilla, Maria Climent, Levent Sarikcioglu, Yesim Senol, Fatos B. Yildirim, Arzu Utuk, Jacek Kunicki, Parichehr Pasbakhsh, Negar Omidi, Hamed Omidi, Fatemeh Dehghani Nazhvani, Seyed Razi Ghalebi, Nima Javan, Akrami Mohagery, Ali Reza Ebrahimzadeh Bideskan, Mohammad Mehdi Hassanzadeh Taheri, Ali Reza Fazel, Cesare Tiengo, Veronica Macchi, Carla Stecco, Andrea Porzionato, Franco Mazzoleni, Raffaele De Caro, Alberto Clemente, Aldo Morra, Pietro Greco, Piero Pavan, Arturo Natali, Mehmet Demir, Mehmet Dokur, Niyazi Acer, Ayfer Mavi, Niki Matveeva, Dobrila Lazarova, Kostandina Korneti, Svetlana Jovevska, Dragica Jurkovik, Meri Papazova, Masoumeh Havasi, Naeim Alboghobeish, Ahmad Savari, Negin Salamat, Mozafar Sharifi, Hyun-Ho Kwak, Kyung-Seok Hu, Gyoo-Cheon Kim, Bong-Soo Park, Hee-Jin Kim, Ahmet Sinav, Adarsh K. Gulati, Nidhi K. Gulati, Hussien Alshammary, Seifollah Dehghani Nazhvani, Amir Vafafar, Tahereh Esmaeilpour, Soghra Bahmanpour, Leila Elyasi, Ahmad Monabbati, M. Ghanadi, Mohammad Reza Paryani, Hassan Gilanpour, Banino Amirsam, Rodrigo Elizondo Omaña, Santos Guzmán López, Oscar De la Garza Castro, Edgar Urrutia Vega, Santos Guzman Lopez, Freshteh Talebpour, Rahim Golmohammadi, Golamreza Dashti, Mohammad Ali Atlasi, Mehdi Mehdizadeh, Mohammad Hadi Bahadori, Mohammad Taghi Joghataei, Leili Hatami, Mandana Beigi Boroujeni, Jasem Estakhr, Ebrahim Esfandiary, Mohsen Marzban, Mehrdad Bakhtiary, Navid Modiry, Mokhtar Jafarpur, Hassan Mofidpur, S. Hassan Alavi, Alareza Mahmoudian, Mohmmad Mohsen Taghavi, Mokhtar Jafarpour, Ali Reza Mahmoudian, Nasrin Sanjarmousavi, Ines Doassans, Natalia Sorrenti, German Decuadro, Andres Saibene, Marie Poumayrac, Sebastian Laza, Carina Almiron, Maria Elena Vergara, Victor Soria, Sebastian Lasa, Adolfo Perez, Gabriela Castro, Ana Santa Maria, Mansoureh Soleimani, Majid Katebi, Masoomeh Bakhshayesh, Mithat Oner, Mehmet Halici, Ali Yikilmaz, Ahmet Guney, Yildirim Turk, Mete Edizer, Umit Beden, Nihal Icten, Mohammad Afshar, Mohammad Mehdi Hasanzadeh Taheri, Adel Moalem, Mohammad Jafar Golalipour, Azadeh Tamizi, Mohammad Ahi, Shahram Mohammadpour, Ardeshir Maiery, Cengiz Acikel, Ersin Ulkur, Huseyin Karagoz, Bahattin Celikoz, Kuldip Bedi, Partadiredja Ginus, Mohammad Jafar Golalipoor, Mohammad Reza Mohammadi, Poya Jhand, Azad Reza Mansourian, Kanizreza Hosseinpoor, Abbas Ali Keshtkar, Raith Alsaffar, Babak Kabiri Balajadeh, Soraya Ghafari, Ramin Azarhosh, Seyyed Amirhossein Fazeli, Mehrdad Jahanshahi, Annen Mohammad Gharravi, Banu Alicioglu, Hakki Muammer Karakas, Ahmet Harma, Hun-Mu Yang, Sung-Yoon Won, Jae-Gi Lee, Ju-Young Lee, Jeong-Yong Lee, Yoo-Ri Kim, Wu-Chul Song, Ki-Seok Koh, Eu-Na Hwang, Hyun-Gon Choi, Soon-Heum Kim, Soo-Young Kim, Mi-Sun Hur, Enis Ulucam, Osman Celbis, Da-Hye Kim, Hee-Suk Hong, Hyun-Joo Kim, Jong-Hoon Choi, Jong-Tae Park, Hyeon-Cheol Kim, Hamed Abbasi, Seyed Mohammad Hosseinipanah, Mohammad Hosseini, A. Amani, H. R. Ashrafi, Mohsen Sadeghimehr, Hyun-Ju Kim, Vadim Sheverdin, Zahra Amani, Alireza Ashrafi, Ali Reza Ashrafi, Hami Javad, Mokhtar Jafarpoor Kachap, Sebastián Laza, Marie Catherine Poumayrac, Inés Doassans, María Elena Vergara, Carina Almirón, Víctor Soria, Alvaro Rivara, Angela Sirilo, Diego Freire, Angela Cirillo, Maria Elena Veragara, Vlado Krmek, Nikola Krmek, Ana Jo-Osvatic, Vasilije Nikolic, Radivoje Radic, R. Shane Tubbs, Marios Loukas, Quentin Fogg, Neil Ashwood, Serpil Cilingiroglu, Cemal Ozbakir, Tahereh Mazoochi, Vedat Sabanciogullari, Cesur Gumus, F. Hayat Erdil, Mehmet Cimen, Hesam Moodi, Fateme Ghiasi, Asghar Akbari, Javad Hami, Majid Khazei, Elham Haghparast, Ioannis Mitsakis, Aikaterini Anastasiou, Menelaos Mitsakis, Kyriaki Sianou, Roxani Hainoglou, Margarida Francisco, Charikleia Mitsaki, Maria Konstantinidi, Stamatia Prapa, Igor Leksan, Tomislav Mrcela, Robert Selthofer, Fatemeh Kermanian, Alireza Mahmoudian, Mahmood Erfanian Ahmadpoor, Naser Dalili, Amir Hossein Elian, Ardesheer Moaiery, Zahra Jamalpour, Mohammad Reza Nourani, Alireza Asgari, Alireza Ebrahimzadeh, Seyed Hasan Eftekharvaghefi, Abbas Mohammadi, Vahid Sheibani, Seyed Noureddin Nematollahi-Mahani, Mastafa Latifpour, Masood Deilami, Behzad Soroure-Azimzadeh, Fatemeh Nabipour, Hamid Najafipour, Nouzar Nakhaee, Mohammad Yaghoobi, Rana Eftekharvaghefi, Parvin Salehinejad, Hasan Azizi, Hamid Reza Riasi, Maliheh Nobakht, Sara Asalgoo, Roshanak Rahbar, Norooz Najafzadeh, Kazem Moosavizadeh, Massood Ezzatabadypour, Masoud Majidi, Reza Malekpor-Afshar, Fariba Karimzade, Mahmood Hoseini, Mohamad Bayat, Ali Gorgi, Akram Nezhadi, Mehrdad Bakhtiari, Homa Rasooli Jazi, Maryam Jafaryan, Hosein Haghir, Mahmood Hosseini, Sadegh Rahimi, Fatemeh Behnam Rassouli, Ali Gorji, Aliasghar Habibi, Fatemeh Pouya, Shahryar Dabiri, A. Mousavi, Saeed Rajabalian, A. Abolidokht, Neda Khanlarkhani, Homayoun Naderian, Nezamedin Berjis, Mohamad Reza Namavar, Tahereh Talaei, Zohreh Mazaheri, Ahmad Monabati, Mehmet Ilkay Kosar, Kezban Karacan, Hamidreza Chegini, Hossein Nikzad, Egemen Ayhan, Sinan Ustundag, Salih Murat Akkin, Tahir Ogut, Parviz Rayegan, Mohamad Ali Emami Meibodi, Reza Montazer Ghaem, Rosa Zargarpoor, Seyd Hasan Eftekhar Vaghefi, Ghazale Moshkdanian, Fateme Poya, Hamid Kohestani, Roozbeh Rayegan Abarghoeai, Parviz Rayegan Abarghoeai, Seyed Hasan Eftekhar Vaghefi, Abolghasem Amir Mahmodi, Ali Poraboli, Hamid Reza Kohestani, Raena Eftekhar Vaghefi, Seyed Hasan Eftekhar Vaghefy, Raena Eftekhar Vaghefy, Parviz Raygan Abarghoeai, Mohamad Saba, Anneh Mohammad Gharravi, Fatemeh Javadnia, Mohsen Zhaleh, Dariush Bijan Nezhad, Mohammad Reza Gholami, Maria Piagkou, Vassiliki Kouki Aikaterini, Giannoulis Piagkos, Stergios Douvetzemis, Panagiotis Skandalakis, Sophia Anagnostopoulou, Nikolaos Papadopoulos, H. Hamdi Celik, Ilkan Tatar, Emel Cadalli Tatar, Burce Ozgen Mocan, Mustafa F. Sargon, C. Cem Denk, Homa Rasoolijazi, Mohammad Taghi Joghataie, Mehrdad Roghani, Salin Murat Akkin, Gulten Dinc, Mustafa Kurklu, Sener Ozboluk, Mahmut Komurcu, Jürgen Koebke, Mehmet Bulent Balioglu, Mehmet Akif Kaygusuz, Ferdi Sefa Bozkus, Ozgur Korkmaz, Sule Biyik Bayram, Mehmet Ali Can, Ebrahim Nasiri, Koroush Jafar-Kazemi, Melina Hosseini, Shahin Maghoul, Mansooreh Soleimani, Abdollah Amini, Mohamad Mahdi Hassanzade, Mohammad Hossein Davari, Tom Van Hoof, Germano T. Gomes, Emmanuel Audenaert, Koenraad Verstraete, Ingrid Kerckaert, Katharina D’Herde, Brion Benninger, Gil Hedley, Florin Mihail Filipoiu, Eugen Tarta, Mihali Enyedi, Cosmin Pantu, Razvan Stanciulescu, Cezary Skobowiat, Jaroslaw Calka, Mariusz Majewski, Maryam Rezaian, Akbar Yaghoobfar, Somayeh Hamedi, and T. Shomali

Subjects
Radiology, Nuclear Medicine and imaging, Surgery, Anatomy, Pathology and Forensic Medicine
Published
2009

4. Transglutaminase and clusterin induction during normal and abnormal limb development in the mouse

Author

S, Adel Moallem and B F, Hales

Subjects
Microscopy, Confocal, Transglutaminases, Apoptosis, Extremities, DNA, Immunohistochemistry, Mice, Clusterin, Teratogens, Pregnancy, Culture Techniques, Animals, Female, Microscopy, Immunoelectron, Cyclophosphamide, Glycoproteins, Molecular Chaperones
Abstract

Apoptotic cell death is important in pattern formation in the limb. The purpose of this study was to investigate the relationship between the occurrence of apoptosis in the mouse limb during normal and abnormal development as well as the expression of tissue transglutaminase and clusterin, two proteins associated with apoptotic cell death. Mouse limb buds were cultured in vitro in the absence or presence of a potent teratogen, an activated analog of cyclophosphamide: 4-hydroperoxycyclophosphamide (1 or 10 micrograms/ml). Using whole-mount in situ DNA labeling and confocal microscopy, apoptotic cells were localized in the interdigital areas of control limbs after culture for 24 h. The number of apoptotic cells in the interdigital areas of the limbs was increased in the presence of 4-hydroperoxycyclophosphamide (1 microgram/ml). Exposure to a higher concentration (10 micrograms/ml) of 4-hydroperoxycyclophosphamide further increased the numbers of cells staining positively for apoptosis. The relative abundance of tissue transglutaminase increased 3-4-fold after 6 or 24 h of culture with either concentration of 4-hydroperoxycyclophosphamide; immunoreactive protein in drug-treated limbs decreased to control levels by 48 h. Transglutaminase immunoreactivity was localized in the interdigital areas of limbs 24 h after drug exposure. Clusterin immunoreactivity in the control limbs was weak. The abundance of clusterin was increased 3-4-fold in drug-treated limbs; this induction occurred only after 48 h of culture with 4-hydroperoxycyclophosphamide. Clusterin immunoreactivity in limbs after drug treatment for 48 h was localized to the interdigital areas; immunogold electron microscopy of clusterin expression showed a specific labeling in phagocytosed apoptotic bodies. Thus, the number of cells staining positively for apoptosis in the limb was greatly increased in the interdigital areas during abnormal limb development. The expression of both transglutaminase and clusterin was altered in areas of the limb undergoing apoptosis during abnormal limb development.

Published
1996

5. On the track of mesothelial progenitor cells from the peritoneal cavity transplanted to immunodeficient mice.

Author

Adel Moallem S and Jahangiri L

Subjects
Animals, Cyclosporine pharmacology, Epithelial Cells physiology, Female, Flow Cytometry methods, Immunologic Deficiency Syndromes metabolism, Immunologic Deficiency Syndromes pathology, In Situ Hybridization, Fluorescence, Male, Mice, Mice, Inbred BALB C, Stem Cell Transplantation, Stem Cells physiology, Time Factors, Cell Culture Techniques, Epithelial Cells cytology, Peritoneal Cavity pathology, Stem Cells cytology
Abstract

Mesothelial progenitor cells have been reported to reside in either the monolayer of mesothelium, submesothelium or within the peritoneal cavity as free floating cells. As a putative plasticity has been reported for the mesothelial progenitor cells and considering the potential implications of the establishment of a novel resource of stem/progenitor cells in gene and cell therapeutics and tissue engineering, we conducted an in vivo tracking of transplanted mesothelial cells. In order to induce immunodeficiency, the recipient mice were treated with 32 mg kg(-1) of daily Cyclosporine. On days 14, 30 and 60 post transplantation, brain, heart, skeletal muscle and lung tissues were screened by a modified FISH method directed to the Y chromosome of donor cells. Fluorescence harboring cells were analyzed by flow cytometry and fluorescent microscopy. The data confirmed by PCR, demonstrated the existence morphology alteration of the donor cells in various organs of the recipient mice, notably in the skeletal muscle and lung and less in the heart and brain. Immunostaining of recovered cells from the nervous recipient tissues suggests differentiation of mesothelial cells in the new microenvironment.

Published
2007
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