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To explore the feasibility of the Internet + nursing service mode in family rehabilitation of elderly patients with osteoarthritic diseases.The control group (n=50) received routine rehabilitation treatment procedures and discharge guidance. For the observation group (n=50), extended nursing rehabilitation service was conducted through the Internet + nursing service platform based on the routine treatment in the control group.(1) The compliance with follow-up of the patients in the observation group was significantly higher than that in the control group; (2) The total satisfaction of patients in the observation group was significantly higher than that in the control group; (3) The VAS (1 month: 4.36±1.15 vs. 5.86±1.61, p0.05; 3 months 4.36±1.15 vs. 5.86±1.61, p0.05), SAS (1 month: 37.21±14.16 vs. 49.31±13.45, p0.05; 3 months 26.73±8.25 vs. 40.33±9.50, p0.05), SDS (1 month: 32.36±10.15 vs. 46.32±12.61, p0.05; 3 months 27.11±8.08 vs. 40.62±11.40, p0.05) and PSQI (1 month: 13.64 ± 1.13 vs. 16.31 ± 3.45, p0.05; 3 months 11.54 ± 1.87 vs. 15.74 ± 1.36, p0.05) scores in the observational group were significantly lower than that in control group at one month and three months after discharge. The ADL (1 month: 86.86 ± 4.13 vs. 74.33 ± 3.44, p0.05; 3 months 90.34 ± 7.87 vs. 78.52 ± 6.36, p0.05) scores in the observational group were significantly higher than that in control group at one month and three months after discharge.The extended rehabilitation nursing management for family rehabilitation of elderly patients with osteoarthritic diseases through the Internet + nursing service is a family rehabilitation model suitable for elderly patients with osteoarthritic diseases in China and has positive significance in developing a diversified medical nursing model.

The standardized training for public health physicians plays an important role in exploring the cultivation public health professionals and strengthening the construction of public health service providers. In 2018, the National Health Commission of China launched a pilot program of standardized training for public health physicians in 10 provinces. This paper clarifies the definition of the standardized training for public health physicians, systematically analyzes the status quo of the training in China and other countries, articulates the design and progress of the training in the perspective of Centers for Disease Control and Prevention, and makes some suggestions for the priorities of the pilot training program, so as to provide reference and basis for the better development of the standardized training for public health physicians in China in the future.公共卫生医师规范化培训是探索公共卫生人才培养、加强公共卫生人才队伍建设的重要途径，国家卫生健康委员会于2018年开始在全国10个省份启动公共卫生医师规范化培训试点。本文从公共卫生医师规范化培训的定义出发，系统分析了国际、国内公共卫生医师规范化培训的现状，解读了疾控机构公共卫生医师规范化培训的设计与进展，并提出试点中重点工作内容的思考与建议，为下一步公共卫生人才培养方向决策提供参考和依据。.

To elucidate the influence of microRNA-125a on the biological behaviors of acute myeloid leukemia (AML) cells.MicroRNA-125a mimic and negative control (NC) were constructed and transfected into AML cell line HL60, respectively. Cell viability of HL60 cells transfected with microRNA-125a mimic or NC was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. Regulatory effects of microRNA-125a on enzyme activities of B-cell lymphoma-2 (Bcl-2), Bcl-xl, caspase-3, and caspase-9 in HL60 cells were quantified by a spectrophotometry. Changes in apoptosis and invasion of HL60 cells overexpressing microRNA-125a were detected by flow cytometry and transwell assay, respectively. Protein levels of cell cycle genes (cyclin B, cdc-2, mdm-2), pro-apoptotic gene p53 and anti-apoptotic gene Bcl-2 in HL60 cells transfected with microRNA-125a mimic or NC were assessed by Western blot. Finally, the mRNA levels of Bax, caspase-8, nuclear factor-κB (NF-κB), and c-myc in HL60 cells with microRNA-125a overexpression were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR).MicroRNA-125a expression remarkably increased by transfection of microRNA-125a mimic into HL60 cells, suggesting its sufficient transfection efficacy. MTT assay revealed an inhibited viability after microRNA-125a overexpression. Transfection of microRNA-125a mimic markedly enhanced enzyme activities of caspase-3 and caspase-9, but reduced activities of Bcl-2 and Bcl-xl in HL60 cells than controls (p0.05). Moreover, microRNA-125a overexpression elevated apoptotic rate as FCM data indicated. Transwell assay demonstrated a decrease in the invasive rate of HL60 cells overexpressing microRNA-125a. Western blot analyses revealed that cell cycle genes all downregulated by transfection of microRNA-125a mimic in HL60 cells. The protein level of p53 upregulated and Bcl-2 downregulated in HL60 cells overexpressing microRNA-125a (p0.05). Furthermore, mRNA levels of pro-apoptotic genes Bax and caspase-8 were enhanced after microRNA-125a overexpression, while mRNA levels of NF-κB and c-myc were reduced (p0.05).MicroRNA-125a inhibits proliferative and invasive potentials, arrests the cell cycle in the G2/M phase of AML cells by regulating the NF-κB pathway.

From June to November 2016, 5 patients with severe burns were admitted to our unit. Broad-spectrum antibiotic and fluconazole were used on patients as earlier empirical anti-infection therapy of bacteria and fungi. Seven to twenty-one days after injury, 5 patients developed fungal infection. Antifungal agents of caspofungin, voriconazole, and amphotericin B liposomewere were used according to the results of fungal culture, and the infected wounds were also treated with repeated debridement and dressing change. Multiple autologous skin grafts were performed after infection control of wounds. With the above antifungal infection treatment for 5 to 11 days, 2 patients' condition tended to be stable, and no fungus was found in wound secretion after cultured for many times. The patients were discharged with wounds healed after 52 to 54 days' hospital stay. Due to severe burns degree and or elder age, fungal infection aggravated and expanded to the trunk in the other 3 patients, then developed into burn sepsis, resulting in patients died of multiple organ failure secondary to sepsis.2016年6—11月，笔者单位收治5例特重度烧伤患者，入院早期经验性应用广谱抗生素抗细菌感染和氟康唑抗真菌感染。伤后7～21 d，5例患者均发生真菌感染，根据真菌培养结果选用卡泊芬净、伏立康唑或两性霉素B脂质体，并对感染创面行多次清创及换药处理，待创面感染控制后，多次行自体皮移植术。经5～11 d积极的抗真菌感染治疗及创面处理后，2例患者病情趋于稳定，创面分泌物多次培养未见真菌，入院后52～54 d创面愈合出院；3例患者因烧伤程度重和/或年龄偏大，真菌感染由四肢创面迅速扩大至躯干，后续发展成烧伤脓毒症导致多脏器功能衰竭而死亡。.

This study was primarily undertaken to test the hypothesis that mitochondrial DNA (mtDNA) mutations may be associated with aplastic anemia (AA). We analyzed mtDNA sequences from 15 patients with AA. The samples were obtained from bone marrow, and patients' oral epithelial cells were collected for normal tissue comparison. Total DNA was amplified by PCR after extraction, and these segments were then sent for sequencing. The results were compared with those of oral epithelial tissues as well as mtDNA sequences in the revised Cambridge Reference Sequence (rCRS) database. We detected 61 heteroplasmic mutations in 11 genes, including those encoding NADH dehydrogenase (ND)1-2 and 4-6, tRNA glutamic acid (TRNE), ribosomal RNA (RNR) 1 and 2, cytochrome c oxidase (COX1), cytochrome b (CYTB), and tRNA glycine (TRNG); mutation rates were particularly high in ND2 (34.4%) and ND4 (21.3%) in the patients' mtDNA genomes. The products of these genes are involved in oxidation in the respiratory chain, and a large number of homoplasmic mutations were found. Interestingly, these 162 polymorphisms were mostly in the D-loop DNA structure (54.3%), in which numerous mutations associated with leukemia and myelodysplastic syndromes are found. We conclude that functional impairment of the mitochondrial respiratory chain induced by mutation may be an important reason for hematopoietic failure in AA patients.

A method was developed to estimate the relative contribution of increased biomass, harvest index and yield components to seed yield gain of soybean. Data from four field experiments conducted in three soybean production regions in China were used to measure the role of different traits (such as harvest index) in seed yield improvement by applying this method. The results indicated that harvest index was a larger contributor to the progress of soybean yield improvements than biomass in China. However, the estimates of improvements of yield components suggested that there could be several strategies for obtaining high yield. High yielding would be achieved by improving all of the three yield components (pods per plant, seeds per pod and seed weight) simultaneously, or significantly improving one or two of the three components.

A capillary zone electrophoresis (CZE) method has been developed for the simultaneous determination of four anthraquinones in Xanthophytum Attopvensis Pierre. The effects of several factors such as the pH, concentration of the running buffer and the separation voltage were investigated to find the optimum conditions. The optimum conditions were an electrolyte containing 15 mmol L−1 borate, at pH 9.64 and 17.5 kV applied voltage. The four analytes were completely separated within 5 min. Regression equations gave linear relationships between the peak area of each analyte and the concentration. The recoveries ranged from 96.5% to 106.2%. The method was successfully used in the analysis of extracts from Xanthophytum Attopvensis Pierre with a relatively simple extraction procedure, and the assay results were satisfactory.

Forty cases of small cell lung carcinoma (SCLC) treated with surgical and chemotherapeutic combined therapy were reported. There were 28 males and 12 females in the group, most with history of more than 2 months, ranging in age of 27-66 years old. Cough, bloody sputum, low fever and chest distress are the main clinical manifestation. The small cell undifferentiated carcinoma was confirmed by fiberbronchoscope examination and pathology in all patients. Single lobectomy was performed in 20 cases, lobectomy of the upper and middle lobe in 9 cases, (sleeve resection of the lobarbronchus in 3 cases), and total pneumonectomy in 7 cases. Two cases were of stage I, 18 were of stage II, and 20 were of stage IIIa. Twelve patients received chemotherapy after operation, and 28 patients underwent the "chemotherapy-operation-chemotherapy" treatment model. Adriamycin (or CDDP), cytoxan, vincristin, and dexomathasone were used for the chemotherapy procedure. The 1, 3, 5-year survival rate of chemotherapy after operation and chemotherapy-operation-chemotherapy group were 70%, 45%, 30.5% and 54%, 30% and 22%, respectively. It is demonstrated that the long-term survival rate could be elevated in SCLC patients treated with chemotherapy after surgical operation, and the chance of operation also could be elevated by preoperative chemotherapy. The resection rate was 93% in the preoperative chemotherapy group.