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Since cellulose is an important element for the production of tissue paper, many researchers used natural-based materials as an alternative in the manufacture of tissue paper so that it will be more environmentally friendly and safe to use for the community. Previous studies have found that cassava peel waste (57% cellulose) or durian peel (60-65%) each can be an alternative raw material for making tissues with quality does not differ much from tissue products in general. This study aims to determine the influence of durian peel and cassava peel mixture composition with a variation of mass ratio as 1:1; 1:1,5 ; 1:2 ; 1:2,5 ; 1:3 ; 1,5:1 ; 2:1 ; 2,5:1 ; 3:1. This research uses sodium hydroxide 10% for chemical pulping and bleached by 2% of hydrogen peroxide. Once the lignin has been rinsed out from the pulp, sheeting should be carried out by adding tapioca, chitosan and virgin coconut oil. Based on the test analysis and referring to SNI 0103:2008, the optimal composition of durian peel and cassava peel mixture is on the mass ratio of 3:1 which has a gramatur value of 41 g/m2, water absorption of 6.3cm, crushed resistance of 46 seconds and color does not fade, with a clean appearance, a little soft and not perforated so that it almost resembles tissues on the market, although it still has some stiffness to the surface.