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Recent studies have provided strong evidence that long-term ethanol consumption leads to activation the mechanisms of neuroimmune signaling. Recently, much attention has been focused on the study of toll-like receptors (Toll-like receptors, TLRs), which play one of the key roles in the mechanisms of activation of the innate immune system in brain structures subsequently ethanol consumption. It is known that the activation of TLRs leads to the release of many proinflammatory cytokines with the resulting neuroinflammatory process. There are suggestions that TLRs may also be involved in the modulation of neurotransmitter systems of the brain, thereby contributing to the formation of pathological dependence on ethanol. The goal of our work was to study the level of expression the genes of TLRs (TLR3, TLR4, TLR7) and pro-inflammatory cytokine genes (IL-1β, CCL2) in the rat brain (amygdala, hippocampus, medial entorhinal cortex, striatum) under conditions of prolonged alcoholization and on different periods of alcohol withdrawal, which was previously not studied by researchers. Prolonged alcoholization of rats with ethanol did not lead to changes in levels mRNA of TLRs in the studied structures of the rat brain, with the exception of a small increase in the level of TLR3 mRNA in the hippocampus of prolonged alcoholized rats and a slight increase in the level of TLR3 mRNA in mEC. However, gene expression of TLRs undergoes changes in all the structures of the rat brain studied by us at different periods of alcohol withdrawal. The increased level of expression of both TLRs and proinflammatory genes in the period of alcohol withdrawal in the rat brain hippocampus deserves special attention, which indicates the presence of a persistent neuroinflammatory process in this brain structure in the period of alcohol withdrawal, which is probably supported with the participation of TLR-dependent signaling. The study of the mechanisms of inflammatory process activation by TLR-dependent signaling in different brain structures can open new targets for drug exposure. Such drugs can be used in the treatment of alcoholism.

Background. Vaccination is considered by the world community as the main measure of the fight against pneumococcal infection [1–3]. Immunization of children has been carried out since the end of 2014. Goals. To study the compliance of the vaccination scheme against pneumococcal infection in young children and its influence on vaccination efficacy.Methodology. Evaluation of the compliance of the vaccination schedule against pneumococcal infection and the impact on morbidity in young children was carried out under the conditions of epidemiological observational descriptive and analytical («cohort») researches. Expert assessment is made of medical records of 52 patients, and 200 electronic medical cards and 200 cards of prophylactic vaccination in children were born in of 2015 and 2016.Results. The high importance of adherence to the vaccination scheme against pneumococcal infection was established not only in the prevention of disease and also in reducing carriage of S. pneumoniae. The greatest effect of pneumococcal immunization was observed in children who received the first vaccination before the age of 6 months and with a completed course of vaccination.Conclusion. Timeliness of immunization of young children against pneumococcal infection is a significant factor in ensuring preventive efficacy of immunization.