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Since the context on environmental issues is challenging, the Cognac protected denomination of origin (PDO) adopted life cycle assessment (LCA) to inform its environmental strategy, initially focusing on vineyard soil management. This study developed a participatory eco-design approach involving stakeholders to inform the PDOs’ strategy for transitioning to less impactful crop management practices. It did this by drawing on literature that emphasises the use of participatory design, serious games, and LCA to foster knowledge sharing, engagement, and sustainability assessment. The approach that was put to the test in the Cognac PDO involved 17 elected officials with varying levels of experience. The participants used the serious game known as Vitigame (a game done for participatory ecodesign in viticulture) to ecodesign a soil management and fertilisation pathway for 2030, with the process guided by the results of the LCA. Exchange dynamics, LCA results, and participant feedback were analysed. The study revealed diverse dynamics and efficient ecodesign strategies among the participant groups, which resulted in a reduction of environmental impacts by up to 51% and raised new questions for the territory. The diversity of participants presented challenges, highlighting the need to improve LCA knowledge sharing. The workshop’s results could inform the PDO’s strategic planning. The interest of this innovative process including a serious game was confirmed, suggesting potential applications to other viticultural PDOs and adaptability to diverse sectors.

Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches are hindered by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in the field of human pluripotent stem cells have fueled the prospect of generating melanocytes. Here, we have developed a well-characterized method to produce a pure and homogenous population of functional and proliferative melanocytes. The genetic stability and potential transformation of melanocytes from pluripotent stem cells have been evaluated over time during the in vitro culture process. Thanks to transcriptomic analysis, the molecular signatures all along the differentiation protocol have been characterized, providing a solid basis for standardizing the protocol. Altogether, our results promise meaningful, broadly applicable, and longer-lasting advances for pigmentation disorders and open perspectives for innovative biotherapies for pigment disorders.