81 results on '"Sáez-Martínez P"'
Search Results
2. Effectiveness evaluation of hyaluronic acid-based commercial eye drops to treat ophthalmic dry eye disease
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Jon Andrade del Olmo, Virginia Sáez Martínez, Nagore Martínez de Cestafe, José María Alonso, Cristina Olavarrieta, Miguel Ucelay López de Heredia, Sandra Benito Cid, and Raúl Pérez González
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Dry eye disease ,Hyaluronic acid molecular weight ,Gamma-polyglutamic acid ,Ex vivo ocular mucoadhesion properties ,DayDrop Advanced® eye drops ,Ophthalmic applications ,Biochemistry ,QD415-436 - Abstract
Nowadays, hyaluronic acid (HA) eye drops are widely used to combat ophthalmic dry eye disease (DED). Till the date, almost all commercialized HA eye drops are single-ingredient despite not displaying significantly different clinical effects (Artelac Splash®). Consequently, scientists are making great efforts to combine HA with other compounds to overcome more successfully DED (trometamol in Hyabak®, sorbitol in HyloComod®). Gamma-polyglutamic acid (γ-PGA) is another novel polymer that could induce a powerful synergistic effect on HA for DED treatment. To the best of our knowledge, eye drops combining HA and γ-PGA neither have been marketed nor scientific studies have been published so far. Fortunately, DayDrop Advanced® eye drop has been recently commercialized with HA + γ-PGA technology. In this work, DayDrop Advanced®, Hyabak®, HyloComod® and Artelac Splash® were compared evaluating their efficacy to treat DED and residence time in eye due to their distinct physicochemical and rheological properties. As key research point, DayDrop Advanced® demonstrated an improved immediate hydration, protection against corneal dehydration, anti-inflammatory, anti-hyaluronidase and ocular mucoadhesion activities than the rest of artificial tears. These phenomena highlight new generation DayDrop Advanced® combining HA + γ-PGA technology as the leading eye drops to treat more durably and effectively DED.
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- 2024
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3. Alternating Current Electrospinning of Polycaprolactone/Chitosan Nanofibers for Wound Healing Applications
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Jon Andrade del Olmo, Petr Mikeš, Nikifor Asatiani, José María Alonso, Virginia Sáez Martínez, and Raúl Pérez González
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electrospinning ,polycaprolactone ,chitosan ,nanofibers ,wound healing ,tissue regeneration ,Organic chemistry ,QD241-441 - Abstract
Traditional wound dressings have not been able to satisfy the needs of the regenerative medicine biomedical area. With the aim of improving tissue regeneration, nanofiber-based wound dressings fabricated by electrospinning (ES) processes have emerged as a powerful approach. Nowadays, nanofiber-based bioactive dressings are mainly developed with a combination of natural and synthetic polymers, such as polycaprolactone (PCL) and chitosan (CHI). Accordingly, herein, PCL/CHI nanofibers have been developed with varying PCL:CHI weight ratios (9:1, 8:2 and 7:3) or CHI viscosities (20, 100 and 600 mPa·s) using a novel alternating current ES (ACES) process. Such nanofibers were thoroughly characterized by determining physicochemical and nanomechanical properties, along with wettability, absorption capacity and hydrolytic plus enzymatic stability. Furthermore, PCL/CHI nanofiber biological safety was validated in terms of cytocompatibility and hemocompatibility (hemolysis < 2%), in addition to a notable antibacterial performance (bacterial reductions of 99.90% for S. aureus and 99.91% for P. aeruginosa). Lastly, the enhanced wound healing activity of PCL/CHI nanofibers was confirmed thanks to their ability to remarkably promote cell proliferation, which make them ideal candidates for long-term applications such as wound dressings.
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- 2024
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4. Resistance to enzymatic degradation and efficacy evaluation of crosslinked hyaluronic acid based commercial viscosupplements for knee osteoarthritis treatment
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Jon Andrade del Olmo, Virginia Sáez Martínez, Nagore Martínez de Cestafe, José María Alonso, Claudia Goenaga Ibeas, Miguel Ucelay López de Heredia, Sandra Benito Cid, and Raúl Pérez González
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Hyaluronic acid ,Commercial injectable hydrogels ,Viscosupplementation ,Knee osteoarthritis ,Traumatology ,Sports medicine ,Biochemistry ,QD415-436 - Abstract
A significant number of people worldwide suffers from knee osteoarthritis (KOA), a chronic and painful disease that is linked to aging, traumatological events and sports medicine. Clinical research indicates that KOA exhibit an altered synovial fluid (SF) with lower endogenous hyaluronic acid (HA) concentration and viscoelastic properties. Nowadays, viscosupplementation by intra-articular HA injections is the most common treatment to restore endogenous HA performance in osteoarthritic SF. In this work, TrHCROSS®, Durolane®, Regenflex®Bio-plus and Monovisc® commercial crosslinked HA viscosupplements were compared in vitro against enzymatic degradation. As key research point, TrHCROSS® demonstrated a considerably increased enzymatic stability compared to the rest of examined viscosupplements, which highlights TrHCROSS® prolonged residence time in joint space reducing more durably and effectively osteoarthritic knee pain. Subsequently, an in-depth physicochemical characterization was realized to support efficacy and stability results of viscosupplements. All in all, TrHCROSS®, developed by SARE® technology, was found to be more successful in treating more durably and effectively KOA based on the remarkable outcomes of conducted trials, which were appropriate findings for the current research investigation.
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- 2023
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5. University Entrepreneurship at the Service of Rural Society. The RuralYU Project as an Action Learning Process
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María Begoña Sáiz Mauleón, Irene Badía Madrigal, Rita Gorriz Salanova, Inés Mondragón Pons, Victoria Olcina Marcos, Pedro Sáez Martínez, Pablo Tortosa Juanes, Guillem García Martín, and María Cos Aznar
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university entrepreneurship ,project-based learning ,social innovation ,rural development ,yudesign ,Technology ,Technology (General) ,T1-995 ,Social Sciences ,Social sciences (General) ,H1-99 - Abstract
The article presents the RuralYU university entrepreneurship project, developed by the Youth University Design (YUDesign) Spontaneous Generation (GE) group of the School of Design Engineering (ETSID), at the Polytechnic University of Valencia (UPV). RuralYU aspires to reduce rural depopulation in collaboration with university students, who, by carrying out academic work, cover specific needs with the aim of strengthening rural development. The idea of an inter-university collaboration programme with the rural world arose after having won the Vodafone Foundation’s Project Lab 2021 national contest within the framework of GLOBAL CHANGE with the proposal “RuralLife4Good. Accommodation grant programme for the empowerment of rural environments”. The winning proposal, which was implemented as a pilot project in Ràfol de Salem during the 2021-2022 academic year, values the importance of the university at the service of society, in this case as an axis of urgent and necessary activation to avoid rural abandonment.
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- 2023
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6. Dysregulation of the miRNome unveils a crosstalk between obesity and prostate cancer: miR-107 asa personalized diagnostic and therapeutic tool
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Vicente Herrero-Aguayo, Prudencio Sáez-Martínez, Juan M. Jiménez-Vacas, M. Trinidad Moreno-Montilla, Antonio J. Montero-Hidalgo, Jesús M. Pérez-Gómez, Juan L. López-Canovas, Francisco Porcel-Pastrana, Julia Carrasco-Valiente, Francisco J. Anglada, Enrique Gómez-Gómez, Elena M. Yubero-Serrano, Alejandro Ibañez-Costa, Aura D. Herrera-Martínez, André Sarmento-Cabral, Manuel D. Gahete, and Raúl M. Luque
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miRNome ,miR-107 ,prostate cancer ,obesity ,non-invasive biomarker ,PSA ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Prostate-specific antigen (PSA) is the gold-standard marker to screen prostate cancer (PCa) nowadays. Unfortunately, its lack of specificity and sensitivity makes the identification of novel tools to diagnose PCa an urgent medical need. In this context, microRNAs (miRNAs) have emerged as potential sources of non-invasive diagnostic biomarkers in several pathologies. Therefore, this study was aimed at assessing for the first time the dysregulation of the whole plasma miRNome in PCa patients and its putative implication in PCa from a personalized perspective (i.e., obesity condition). Plasma miRNome from a discovery cohort (18 controls and 19 PCa patients) was determined using an Affymetrix-miRNA array, showing that the expression of 104 miRNAs was significantly altered, wherein six exhibited a significant receiver operating characteristic (ROC) curve to distinguish between control and PCa patients (area under the curve [AUC] = 1). Then, a systematic validation using an independent cohort (135 controls and 160 PCa patients) demonstrated that miR-107 was the most profoundly altered miRNA in PCa (AUC = 0.75). Moreover, miR-107 levels significantly outperformed the ability of PSA to distinguish between control and PCa patients and correlated with relevant clinical parameters (i.e., PSA). These differences were more pronounced when considering only obese patients (BMI > 30). Interestingly, miR-107 levels were reduced in PCa tissues versus non-tumor tissues (n = 84) and in PCa cell lines versus non-tumor cells. In vitro miR-107 overexpression altered key aggressiveness features in PCa cells (i.e., proliferation, migration, and tumorospheres formation) and modulated the expression of important genes involved in PCa pathophysiology (i.e., lipid metabolism [i.e., FASN] and splicing process). Altogether, miR-107 might represent a novel and useful personalized diagnostic and prognostic biomarker and a potential therapeutic tool in PCa, especially in obese patients.
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- 2022
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7. Nanometric Hydroxyapatite Particles as Active Ingredient for Bioinks: A Review
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Edilberto Ojeda, África García-Barrientos, Nagore Martínez de Cestafe, José María Alonso, Raúl Pérez-González, and Virginia Sáez-Martínez
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additive manufacturing ,3D printing ,bioprinting ,bone cements ,hydroxyapatite ,biomimetic ,Chemical technology ,TP1-1185 ,Biochemistry ,QD415-436 - Abstract
Additive manufacturing (AM), frequently cited as three-dimensional (3D) printing, is a relatively new manufacturing technique for biofabrication, also called 3D manufacture with biomaterials and cells. Recent advances in this field will facilitate further improvement of personalized healthcare solutions. In this regard, tailoring several healthcare products such as implants, prosthetics, and in vitro models, would have been extraordinarily arduous beyond these technologies. Three-dimensional-printed structures with a multiscale porosity are very interesting manufacturing processes in order to boost the capability of composite scaffolds to generate bone tissue. The use of biomimetic hydroxyapatite as the main active ingredient for bioinks is a helpful approach to obtain these advanced materials. Thus, 3D-printed biomimetic composite designs may produce supplementary biological and physical benefits. Three-dimensional bioprinting may turn to be a bright solution for regeneration of bone tissue as it enables a proper spatio-temporal organization of cells in scaffolds. Different types of bioprinting technologies and essential parameters which rule the applicability of bioinks are discussed in this review. Special focus is made on hydroxyapatite as an active ingredient for bioinks design. The goal of such bioinks is to reduce the constraints of commonly applied treatments by enhancing osteoinduction and osteoconduction, which seems to be exceptionally promising for bone regeneration.
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- 2022
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8. The circularity of the business model and the performance of bioeconomy firms: An interactionist business-environment model
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Alberto Alcalde-Calonge, Pablo Ruiz-Palomino, and Francisco José Sáez-Martínez
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circular economy ,circularity level ,business/environment factors ,social capital ,entrepreneurial orientation ,Business ,HF5001-6182 ,Management. Industrial management ,HD28-70 - Abstract
Drawing on the Sustainable Development Goals of the UN 2030 Agenda, the implementation of circular economy business models (CEBMs) is key for reducing the impacts of business production modes on the environment and is especially suited to, and necessary in, the bioeconomy sector. The purpose of this literature review article is to analyse how firms in this sector can achieve a higher level of circularity in their business models, and how this circularity is positive for the triple bottom line. Building on the theories of social capital, new institutionalism and the triple bottom line (TBL), this article proposes a model to better understand how forest bioeconomy firms can develop high-circularity business models that lead them to excel on the TBL. This study combines internal aspects of the business (e.g., social capital, dynamic capabilities, entrepreneurial orientation) with situational variables (social/cultural, regulatory, technological, economic/financial drivers) to explain the level of circularity of business models in bioeconomy firms, as a prior step to enhancing their TBL. The interactionist model proposed is new to the literature and improves our understanding of how the level of circularity of a BM can be enhanced. The propositions derived will help to structure and design future research.
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- 2022
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9. Combining grazing incidence X-rays and micro-diffraction for qualitative phase identification in forensic powdered micro-samples
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Sáez-Martínez, P., Gárate-Lagos, J., Camargo, S., Torres-Roquer, F., Queralt, I., and Salazar-Kuri, U.
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- 2021
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10. Surgical Technique of a Cement-On-Cement Removal System for Hip and Knee Arthroplasty Revision Surgery
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Miguel Tovar-Bazaga, MD, David Sáez-Martínez, MD, Álvaro Auñón, MD, PhD, Felipe López-Oliva, MD, Belén Pardos-Mayo, MD, and Emilio Calvo, MD, PhD
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Total knee arthroplasty ,Total hip arthroplasty ,Revision ,Cement removal ,Infection ,Metal cemover ,Orthopedic surgery ,RD701-811 - Abstract
Cement removal during hip or knee arthroplasty revision surgery is technically demanding and prone to severe complications such as periprosthetic fractures, incomplete cement removal, or perforations. Several alternative techniques have been developed to enable complete, accurate, and safe removal of cement from bone, including osteotomies and cortical windows, endoscopic instruments, ultrasound devices, lithotripsy, and laser-assisted removal. We describe a cement-on-cement technique with a sterile, single-use tool for cement removal. The cement is removed piece by piece using a specifically designed device, without osteotomies or cortical windows.
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- 2021
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11. Granular Disulfide-Crosslinked Hyaluronic Hydrogels: A Systematic Study of Reaction Conditions on Thiol Substitution and Injectability Parameters
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Luis Andrés Pérez, Rebeca Hernández, José María Alonso, Raúl Pérez-González, and Virginia Sáez-Martínez
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injectable hydrogel ,hyaluronic acid ,thiol ,microgels ,granular hydrogels ,Organic chemistry ,QD241-441 - Abstract
Granular polymer hydrogels based on dynamic covalent bonds are attracting a great deal of interest for the design of injectable biomaterials. Such materials generally exhibit shear-thinning behavior and properties of self-healing/recovery after the extrusion that can be modulated through the interactions between gel microparticles. Herein, bulk macro-hydrogels based on thiolated-hyaluronic acid were produced by disulphide bond formation using oxygen as oxidant at physiological conditions and gelation kinetics were monitored. Three different thiol substitution degrees (SD%: 65%, 30% and 10%) were selected for hydrogel formation and fully characterized as to their stability in physiological medium and morphology. Then, extrusion fragmentation technique was applied to obtain hyaluronic acid microgels with dynamic disulphide bonds that were subsequently sterilized by autoclaving. The resulting granular hyaluronic hydrogels were able to form stable filaments when extruded through a syringe. Rheological characterization and cytotoxicity tests allowed to assess the potential of these materials as injectable biomaterials. The application of extrusion fragmentation for the formation of granular hyaluronic hydrogels and the understanding of the relation between the autoclaving processes and the resulting particle size and rheological properties should expand the development of injectable materials for biomedical applications.
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- 2023
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12. Normalizing rings
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García-Pacheco, Francisco Javier and Sáez-Martínez, Sol
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- 2020
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13. Somatostatin, Cortistatin and Their Receptors Exert Antitumor Actions in Androgen-Independent Prostate Cancer Cells: Critical Role of Endogenous Cortistatin
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Prudencio Sáez-Martínez, Francisco Porcel-Pastrana, Jesús M. Pérez-Gómez, Sergio Pedraza-Arévalo, Enrique Gómez-Gómez, Juan M. Jiménez-Vacas, Manuel D. Gahete, and Raúl M. Luque
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somatostatin ,cortistatin ,prostate cancer ,somatostatin analogues ,therapeutic tool ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Somatostatin (SST), cortistatin (CORT), and their receptors (SSTR1-5/sst5TMD4-TMD5) comprise a multifactorial hormonal system involved in the regulation of numerous pathophysiological processes. Certain components of this system are dysregulated and play critical roles in the development/progression of different endocrine-related cancers. However, the presence and therapeutic role of this regulatory system in prostate cancer (PCa) remain poorly explored. Accordingly, we performed functional (proliferation/migration/colonies-formation) and mechanistic (Western-blot/qPCR/microfluidic-based qPCR-array) assays in response to SST and CORT treatments and CORT-silencing (using specific siRNA) in different PCa cell models [androgen-dependent (AD): LNCaP; androgen-independent (AI)/castration-resistant PCa (CRPC): 22Rv1 and PC-3], and/or in the normal-like prostate cell-line RWPE-1. Moreover, the expression of SST/CORT system components was analyzed in PCa samples from two different patient cohorts [internal (n = 69); external (Grasso, n = 88)]. SST and CORT treatment inhibited key functional/aggressiveness parameters only in AI-PCa cells. Mechanistically, antitumor capacity of SST/CORT was associated with the modulation of oncogenic signaling pathways (AKT/JNK), and with the significant down-regulation of critical genes involved in proliferation/migration and PCa-aggressiveness (e.g., MKI67/MMP9/EGF). Interestingly, CORT was highly expressed, while SST was not detected, in all prostate cell-lines analyzed. Consistently, endogenous CORT was overexpressed in PCa samples (compared with benign-prostatic-hyperplasia) and correlated with key clinical (i.e., metastasis) and molecular (i.e., SSTR2/SSTR5 expression) parameters. Remarkably, CORT-silencing drastically enhanced proliferation rate and blunted the antitumor activity of SST-analogues (octreotide/pasireotide) in AI-PCa cells. Altogether, we provide evidence that SST/CORT system and SST-analogues could represent a potential therapeutic option for PCa, especially for CRPC, and that endogenous CORT could act as an autocrine/paracrine regulator of PCa progression.
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- 2022
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14. Pleurectomía parietal y decorticación pulmonar por cirugía torácica asistida por video (VATS) monopuerto versus toracotomía
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Anuar Alonso Sáez-Martínez, Daniel José Jaller-Salleg, Enrique Carlos Ramos-Clason, and Paula Andrea Usta-Tirado
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cirugía torácica ,cirugía torácica asistida por video ,toracotomía ,lobectomía ,decorticación pleural ,evaluación de resultados de intervenciones terapéuticas ,Surgery ,RD1-811 - Abstract
Introducción. El avance de la cirugía torácica abierta a cirugía torácica asistida por vídeo por tres puertos, y sus posteriores efectos en la recuperación de los pacientes, conllevó al desarrollo de la técnica por un solo puerto, que ha mostrado beneficios en el postoperatorio. El objetivo de este estudio fue comparar los resultados postquirúrgicos de los pacientes sometidos a pleurectomía parietal y decorticación pulmonar toracoscópica asistida por video monopuerto y los obtenidos por toracotomía convencional, en una clínica de cuarto nivel, entre 2016 y 2019. Métodos. Estudio descriptivo, en el que se incluyeron 79 pacientes llevados a pleurectomía parietal y decorticación pulmonar por toracoscopia asistida por vídeo monopuerto y 25 pacientes operados por toracotomía convencional. Se evaluaron variables sociodemográficas, clínicas y postoperatorias. Se utilizaron las pruebas de Chi2 o de Fisher y las pruebas t de Student y Mann Whitney. Resultados. La mediana de edad fue menor en el grupo de pacientes operados por toracotomía convencional (28 años, RIC: 26–48, p=0,0005). No hubo diferencia en los tiempos quirúrgicos. Se encontró menor intensidad del dolor y disminución en los días con tubo de tórax, uso de antibióticos, días de UCI y días de estancia hospitalaria en el grupo de pacientes operados por toracoscopia asistida por vídeo monopuerto (p
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- 2021
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15. Cooperation With Universities in the Development of Eco-Innovations and Firms’ Performance
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Juan J. Arroyave, Francisco J. Sáez-Martínez, and Ángela González-Moreno
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value cocreation ,eco-innovation ,operational flexibility ,performance ,cooperation with universities ,Psychology ,BF1-990 - Abstract
In recent decades, the expansion of economic activity has been accompanied by negative environmental impacts. In response, there have been dramatic changes worldwide in terms of an increased demand for environmentally friendly products and services. To achieve these eco-innovations, firms have sought to acquire knowledge and implement operational flexibility by cooperating with different agents such as universities through a value cocreation system that is also expected to enhance firms’ performance. Using a sample of 250 companies, the present paper examines the role of cooperation with universities in the development of diverse environmental innovations and building operational flexibility and, through this, improving firm performance. Results show that firms that value cooperation with universities develop a wider range of environmental innovations and increase their sales and benefits.
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- 2020
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16. Drug Delivery from Hyaluronic Acid–BDDE Injectable Hydrogels for Antibacterial and Anti-Inflammatory Applications
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Jon Andrade del Olmo, Leyre Pérez-Álvarez, Virginia Sáez Martínez, Sandra Benito Cid, Raúl Pérez González, José Luis Vilas-Vilela, and José María Alonso
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hyaluronic acid ,injectable hydrogels ,biocompatibility ,drug delivery ,antibacterial ,anti-inflammatory ,Science ,Chemistry ,QD1-999 ,Inorganic chemistry ,QD146-197 ,General. Including alchemy ,QD1-65 - Abstract
Hyaluronic acid (HA) injectable biomaterials are currently applied in numerous biomedical areas, beyond their use as dermal fillers. However, bacterial infections and painful inflammations are associated with healthcare complications that can appear after injection, restricting their applicability. Fortunately, HA injectable hydrogels can also serve as drug delivery platforms for the controlled release of bioactive agents with a critical role in the control of certain diseases. Accordingly, herein, HA hydrogels were crosslinked with 1 4-butanediol diglycidyl ether (BDDE) loaded with cefuroxime (CFX), tetracycline (TCN), and amoxicillin (AMX) antibiotics and acetylsalicylic acid (ASA) anti-inflammatory agent in order to promote antibacterial and anti-inflammatory responses. The hydrogels were thoroughly characterized and a clear correlation between the crosslinking grade and the hydrogels’ physicochemical properties was found after rheology, scanning electron microscopy (SEM), thermogravimetry (TGA), and differential scanning calorimetry (DSC) analyses. The biological safety of the hydrogels, expected due to the lack of BDDE residues observed in 1H-NMR spectroscopy, was also corroborated by an exhaustive biocompatibility test. As expected, the in vitro antibacterial and anti-inflammatory activity of the drug-loaded HA-BDDE hydrogels was confirmed against Staphylococcus aureus by significantly decreasing the pro-inflammatory cytokine levels.
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- 2022
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17. Spontaneous Gelation of Adhesive Catechol Modified Hyaluronic Acid and Chitosan
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Guillermo Conejo-Cuevas, Leire Ruiz-Rubio, Virginia Sáez-Martínez, Raul Pérez-González, Oihane Gartziandia, Amaia Huguet-Casquero, and Leyre Pérez-Álvarez
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hyaluronic acid ,chitosan ,catechol ,tissue adhesive ,Organic chemistry ,QD241-441 - Abstract
Spontaneously formed hydrogels are attracting increasing interest as injectable or wound dressing materials because they do not require additional reactions or toxic crosslinking reagents. Highly valuable properties such as low viscosity before external application, adequate filmogenic capacity, rapid gelation and tissue adhesion are required in order to use them for those therapeutic applications. In addition, biocompatibility and biodegradability are also mandatory. Accordingly, biopolymers, such as hyaluronic acid (HA) and chitosan (CHI), that have shown great potential for wound healing applications are excellent candidates due to their unique physiochemical and biological properties, such as moisturizing and antimicrobial ability, respectively. In this study, both biopolymers were modified by covalent anchoring of catechol groups, and the obtained hydrogels were characterized by studying, in particular, their tissue adhesiveness and film forming capacity for potential skin wound healing applications. Tissue adhesiveness was related to o-quinone formation over time and monitored by visible spectroscopy. Consequently, an opposite effect was observed for both polysaccharides. As gelation advances for HA-CA, it becomes more adhesive, while competitive reactions of quinone in CHI-CA slow down tissue adhesiveness and induce a detriment of the filmogenic properties.
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- 2022
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18. Contaminated sites, waste management, and green chemistry: new challenges from monitoring to remediation
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Higueras, Pablo L., Sáez-Martínez, Francisco J., Lefebvre, Gilles, and Moilleron, Régis
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- 2019
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19. Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer
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Juan M. Jiménez-Vacas, Vicente Herrero-Aguayo, Antonio J. Montero-Hidalgo, Enrique Gómez-Gómez, Antonio C. Fuentes-Fayos, Antonio J. León-González, Prudencio Sáez-Martínez, Emilia Alors-Pérez, Sergio Pedraza-Arévalo, Teresa González-Serrano, Oscar Reyes, Ana Martínez-López, Rafael Sánchez-Sánchez, Sebastián Ventura, Elena M. Yubero-Serrano, María J. Requena-Tapia, Justo P. Castaño, Manuel D. Gahete, and Raúl M. Luque
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Medicine ,Medicine (General) ,R5-920 - Abstract
Background: Dysregulation of splicing variants (SVs) expression has recently emerged as a novel cancer hallmark. Although the generation of aberrant SVs (e.g. AR-v7/sst5TMD4/etc.) is associated to prostate-cancer (PCa) aggressiveness and/or castration-resistant PCa (CRPC) development, whether the molecular reason behind such phenomena might be linked to a dysregulation of the cellular machinery responsible for the splicing process [spliceosome-components (SCs) and splicing-factors (SFs)] has not been yet explored. Methods: Expression levels of 43 key SCs and SFs were measured in two cohorts of PCa-samples: 1) Clinically-localized formalin-fixed paraffin-embedded PCa-samples (n = 84), and 2) highly-aggressive freshly-obtained PCa-samples (n = 42). Findings: A profound dysregulation in the expression of multiple components of the splicing machinery (i.e. 7 SCs/19 SFs) were found in PCa compared to their non-tumor adjacent-regions. Notably, overexpression of SNRNP200, SRSF3 and SRRM1 (mRNA and/or protein) were associated with relevant clinical (e.g. Gleason score, T-Stage, metastasis, biochemical recurrence, etc.) and molecular (e.g. AR-v7 expression) parameters of aggressiveness in PCa-samples. Functional (cell-proliferation/migration) and mechanistic [gene-expression (qPCR) and protein-levels (western-blot)] assays were performed in normal prostate cells (PNT2) and PCa-cells (LNCaP/22Rv1/PC-3/DU145 cell-lines) in response to SNRNP200, SRSF3 and/or SRRM1 silencing (using specific siRNAs) revealed an overall decrease in proliferation/migration-rate in PCa-cells through the modulation of key oncogenic SVs expression levels (e.g. AR-v7/PKM2/XBP1s) and alteration of oncogenic signaling pathways (e.g. p-AKT/p-JNK). Interpretation: These results demonstrate that the spliceosome is drastically altered in PCa wherein SNRNP200, SRSF3 and SRRM1 could represent attractive novel diagnostic/prognostic and therapeutic targets for PCa and CRPC. Keywords: Prostate cancer, Splicing, Spliceosome, SNRNP200, SRSF3, SRRM1, Therapeutic target
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- 2020
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20. Comparative Cytotoxic Activity of Hydroxytyrosol and Its Semisynthetic Lipophilic Derivatives in Prostate Cancer Cells
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Antonio J. León-González, Prudencio Sáez-Martínez, Juan M. Jiménez-Vacas, Vicente Herrero-Aguayo, Antonio J. Montero-Hidalgo, Enrique Gómez-Gómez, Andrés Madrona, Justo P. Castaño, José L. Espartero, Manuel D. Gahete, and Raúl M. Luque
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anticancer ,extra virgin olive oil ,hydroxytyrosol ,prostate cancer ,semisynthetic derivatives ,Therapeutics. Pharmacology ,RM1-950 - Abstract
A high adherence to a Mediterranean diet has been related to numerous beneficial effects in human health, including a lower incidence and mortality of prostate cancer (PCa). Olive oil is an important source of phenolic bioactive compounds, mainly hydroxytyrosol (HT), of this diet. Because of the growing interest of this compound and its derivatives as a cancer chemopreventive agent, we aimed to compare the in vitro effect of HT isolated from olive mill wastewaters and five semisynthetic alkyl ether, ester, and nitro-derivatives against prostate cancer (PCa) cell lines. The effect in cell proliferation was determined in RWPE-1, LNCaP, 22Rv1, and PC-3 cells by resazurin assay, the effect in cell migration by wound healing assay, and tumorsphere and colony formation were evaluated. The changes in key signaling pathways involved in carcinogenesis were assessed by using a phosphorylation pathway profiling array and by Western blotting. Antiproliferative effects of HT and two lipophilic derivatives [hydroxytyrosyl acetate (HT-Ac)/ethyl hydroxytyrosyl ether (HT-Et)] were significantly higher in cancerous PC-3 and 22Rv1 cells than in non-malignant RWPE-1 cells. HT/HT-Ac/HT-Et significantly reduced migration capacity in RWPE-1 and PC-3 and prostatosphere size and colony formation in 22Rv1, whereas only HT-Ac and HT-Et reduced these functional parameters in PC-3. The cytotoxic effect in 22Rv1 cells was correlated with modifications in the phosphorylation pattern of key proteins, including ERK1/2 and AKT. Consistently, HT-Ac and HT-Et decreased p-AKT levels in PC-3. In sum, our results suggest that HT and its lipophilic derivatives could be considered as potential therapeutic tools in PCa.
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- 2021
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21. Hyaluronic Acid Hydrogels Crosslinked in Physiological Conditions: Synthesis and Biomedical Applications
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Luis Andrés Pérez, Rebeca Hernández, José María Alonso, Raúl Pérez-González, and Virginia Sáez-Martínez
- Subjects
hyaluronic acid ,cross-linking ,physiological conditions ,Biology (General) ,QH301-705.5 - Abstract
Hyaluronic acid (HA) hydrogels display a wide variety of biomedical applications ranging from tissue engineering to drug vehiculization and controlled release. To date, most of the commercially available hyaluronic acid hydrogel formulations are produced under conditions that are not compatible with physiological ones. This review compiles the currently used approaches for the development of hyaluronic acid hydrogels under physiological/mild conditions. These methods include dynamic covalent processes such as boronic ester and Schiff-base formation and click chemistry mediated reactions such as thiol chemistry processes, azide-alkyne, or Diels Alder cycloaddition. Thermoreversible gelation of HA hydrogels at physiological temperature is also discussed. Finally, the most outstanding biomedical applications are indicated for each of the HA hydrogel generation approaches.
- Published
- 2021
- Full Text
- View/download PDF
22. Normalizing rings
- Author
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García-Pacheco, Francisco Javier and Sáez-Martínez, Sol
- Abstract
In this manuscript, we transport the classical Operator Theory on complex Banach spaces to normed modules over absolutely valued rings. In some cases, we are able to extend classical results on complex Banach spaces to normed modules over normed rings. In order to make sure that bounded linear maps on normed modules coincide with the continuous linear maps, it is sufficient that the underlying ring be practical(a topological ring is practical if the invertibles approach zero). In order for other classical results to work on the scope of normed modules, it is sufficient that the underlying ring be normalizing. Normalizing rings is a new class of rings introduced in this manuscript. We provide a characterization of such rings as well as nontrivial examples.
- Published
- 2024
- Full Text
- View/download PDF
23. Effectiveness of interceptive treatment of class III malocclusions with skeletal anchorage: A systematic review and meta-analysis.
- Author
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Jorge Rodríguez de Guzmán-Barrera, Carla Sáez Martínez, Montserrat Boronat-Catalá, Jose María Montiel-Company, Vanessa Paredes-Gallardo, José Luís Gandía-Franco, José Manuel Almerich-Silla, and Carlos Bellot-Arcís
- Subjects
Medicine ,Science - Abstract
Recently, new strategies for treating class III malocclusions have appeared. Skeletal anchorage appears to reduce the dentoalveolar effects while maximising the orthopaedic effect in growing patients. The purpose of this systematic review and meta-analysis is to examine the effectiveness of bone anchorage devices for interceptive treatment of skeletal class III malocclusions. Searches were made in the Pubmed, Embase, Scopus and Cochrane databases, as well as in a grey literature database, and were complemented by hand-searching. The criteria for eligibility were: patients who had undergone orthodontic treatment with skeletal anchorage (miniplates and miniscrews). Patients with syndromes or craniofacial deformities or who had undergone maxillofacial surgery were excluded. The following variables were recorded for each article: author, year of publication, type of study, sample size, dropouts, demographic variables, treatment carried out, radiographic study (2D or 3D), follow-up time, and quality of the articles on the Newcastle-Ottawa Scale. The means and confidence intervals of the following variables were employed: Wits, overjet, ANB, SNA and SNB. Initially, 239 articles were identified. After removing the duplicates and applying the selection criteria, 9 were included in the qualitative synthesis and 7 in the quantitative synthesis (meta-analysis). It may be concluded that skeletal anchorage is an effective treatment for improving skeletal Class III malocclusion, but when compared with other traditional treatments such as disjunction and face mask, there is no clear evidence that skeletal anchorage improves the results.
- Published
- 2017
- Full Text
- View/download PDF
24. Short-term treatment outcome of pulpotomies in primary molars using mineral trioxide aggregate and Biodentine: a randomized clinical trial
- Author
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Cuadros-Fernández, C., Lorente Rodríguez, A. I., Sáez-Martínez, S., García-Binimelis, J., About, I., and Mercadé, M.
- Published
- 2016
- Full Text
- View/download PDF
25. Synthesis and Characterization of Covalently Crosslinked pH-Responsive Hyaluronic Acid Nanogels: Effect of Synthesis Parameters
- Author
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Sheila Maiz-Fernández, Leyre Pérez-Álvarez, Leire Ruiz-Rubio, Raúl Pérez González, Virginia Sáez-Martínez, Jesica Ruiz Pérez, and José Luis Vilas-Vilela
- Subjects
hyaluronic acid ,nanogels ,divinyl sulfone ,1,4-butanediol diglycidyl ether ,poly(ethylene glycol) bis(amine) ,Organic chemistry ,QD241-441 - Abstract
Stable hyaluronic acid nanogels were obtained following the water-in-oil microemulsion method by covalent crosslinking with three biocompatible crosslinking agents: Divinyl sulfone, 1,4-butanediol diglycidyl ether (BDDE), and poly(ethylene glycol) bis(amine). All nanoparticles showed a pH-sensitive swelling behavior, according to the pKa value of hyaluronic acid, as a consequence of the ionization of the carboxylic moieties, as it was corroborated by zeta potential measurements. QELS studies were carried out to study the influence of the chemical structure of the crosslinking agents on the particle size of the obtained nanogels. In addition, the effect of the molecular weight of the biopolymer and the degree of crosslinking on the nanogels dimensions was also evaluated for BDDE crosslinked nanoparticles, which showed the highest pH-responsive response.
- Published
- 2019
- Full Text
- View/download PDF
26. Tumor suppressor role of RBM22 in prostate cancer acting as a dual-factor regulating alternative splicing and transcription of key oncogenic genes.
- Author
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Jiménez-Vacas, Juan M., Montero-Hidalgo, Antonio J., Gómez-Gómez, Enrique, Sáez-Martínez, Prudencio, Fuentes-Fayos, Antonio C., Closa, Adrià, González-Serrano, Teresa, Martínez-López, Ana, Sánchez-Sánchez, Rafael, López-Casas, Pedro P., Sarmento-Cabral, André, Olmos, David, Eyras, Eduardo, Castaño, Justo P., Gahete, Manuel D., and Luque, Raul M.
- Abstract
Prostate cancer (PCa) is one of the leading causes of cancer-related deaths among men. Consequently, the identification of novel molecular targets for treatment is urgently needed to improve patients' outcomes. Our group recently reported that some elements of the cellular machinery controlling alternative-splicing might be useful as potential novel therapeutic tools against advanced PCa. However, the presence and functional role of RBM22, a key spliceosome component, in PCa remains unknown. Therefore, RBM22 levels were firstly interrogated in 3 human cohorts and 2 preclinical mouse models (TRAMP/Pbsn-Myc). Results were validated in in silico using 2 additional cohorts. Then, functional effects in response to RBM22 overexpression (proliferation, migration, tumorspheres/colonies formation) were tested in PCa models in vitro (LNCaP, 22Rv1, and PC-3 cell-lines) and in vivo (xenograft). High throughput methods (ie, RNA-seq, nCounter PanCancer Pathways Panel) were performed in RBM22 overexpressing cells and xenograft tumors. We found that RBM22 levels were down-regulated (mRNA and protein) in PCa samples, and were inversely associated with key clinical aggressiveness features. Consistently, a gradual reduction of RBM22 from non-tumor to poorly differentiated PCa samples was observed in transgenic models (TRAMP/Pbsn-Myc). Notably, RBM22 overexpression decreased aggressiveness features in vitro , and in vivo. These actions were associated with the splicing dysregulation of numerous genes and to the downregulation of critical upstream regulators of cell-cycle (i.e., CDK1/CCND1/EPAS1). Altogether, our data demonstrate that RBM22 plays a critical pathophysiological role in PCa and invites to suggest that targeting negative regulators of RBM22 expression/activity could represent a novel therapeutic strategy to tackle this disease. RBM22 is downregulated in PCa, and its levels (RNA and protein) are inversely associated with tumor-aggressiveness features in human cohorts and transgenic mice models. RBM22 overexpression reduces aggressiveness features in PCa models in vitro and in vivo, through the dysregulation of the splicing process (i.e., increasing alternative first exon and exon skipping splicing events) and downregulation of CDK1, EPAS1 and ATR genes [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
27. Assessing the impact of the “Entrepreneurs” education programme on participants’ entrepreneurial intentions
- Author
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Díaz-García, Cristina, Sáez-Martínez, Francisco, and Jiménez-Moreno, Juan
- Published
- 2015
- Full Text
- View/download PDF
28. ILUMINAR LA PENUMBRA: DECADENTISMO Y HOMOEROTISMO EN EL MARTIRIO DE SAN SEBASTIÁN DE ANTONIO DE HOYOS Y VINENT.
- Author
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Sáez Martínez, Begoña
- Subjects
SEX work - Abstract
Copyright of Siglo Diecenueve: Literatura Hispánica is the property of Universitas Castellae and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
29. Understanding Pay Satisfaction: Effects of Supervisor Ethical Leadership on Job Motivating Potential Influence
- Author
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Ruiz-Palomino, Pablo, Sáez-Martínez, Francisco J., and Martínez-Cañas, Ricardo
- Published
- 2013
- Full Text
- View/download PDF
30. Reseñas De Libros
- Author
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Túa Blesa, José Antonio Llera, Luis Beltrán Almería, Ángel Luis Lujan, Joaquín Álvarez Barrientos, Francisco López Estrada, Josep María Sala Valldaura, Laura Aránzazu Ponce García, Lucía Díaz Marroquín, Armando Pego Puigbó, Petra García Cerro, Rafael Bonilla Cerezo, Marta Giné Janer, José Checa Beltrán, Pablo Ramos Glez. Del Rivero, María Ángulo Egea, Remedios Sánchez, Beatriz Ferrús Antón, Julia María Labrador Ben, Alberto Sánchez Álvarez-Insúa, Begoña Sáez Martínez, Rafael Alargón Sierra, María Jesús Moreno Sou's, Rosamna Pardellas Velay, Sofía Eiroa Rodríguez, and José Luis García Barrientos
- Subjects
French literature - Italian literature - Spanish literature - Portuguese literature ,PQ1-3999 - Published
- 2004
31. Reseñas de Libros
- Author
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M. Ángeles Sanz Manzano, Marcial Rubio Arquez, Óscar Cornago Bernal, Bernardo Antonio González, Ángel Luis Lujan, Roberto Castilla Pérez, Franco Quinziano, Miguel Á. Lozano Marco, Paloma Albalá, Ignacio Pérez Ibáñez, Armando Pego Puigbó, Rebeca Sanmartín Bastida, Carmen Castillejo Larrubia, José Checa Beltrán, Raquel García Pascual, Iñigo Sánchez Llama, Rosamna Pardellas Velay, José María Balcells, María Ángulo Egea, Marco Herrera Campos, Íñigo Sánchez Llama, Rafael Bonilla Cerezo, Beatriz Ferrús Antón, Begoña Sáez Martínez, José Antonio Llera, Joaquín Álvarez Barrientos, Luis Alburquerque García, M. Isabel, Esther Gómez Sierra, José Luis García Barrientos, Fernando Romo Feito, José A. Esgrig Aparicio, and Patrizia Garelli
- Subjects
French literature - Italian literature - Spanish literature - Portuguese literature ,PQ1-3999 - Published
- 2003
32. Why European Entrepreneurs in the Water and Waste Management Sector Are Willing to Go beyond Environmental Legislation
- Author
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Adrián Rabadán and Francisco J. Sáez-Martínez
- Subjects
water sector ,SMEs ,environmental responsibility ,drivers ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
Sustainability in the water sector in Europe is a major concern, and compliance with the current legislation alone does not seem to be enough to face major challenges like climate change or population growth and concentration. The greatest potential for improvement appears when companies decide to take a step forward and go beyond environmental legislation. This study focuses on the environmental responsibility (ER) of European small and medium-sized enterprises (SMEs) in the water and waste management sector and analyzes the drivers that lead these firms to the adoption of more sustainable practices. Our results show that up to 40% of European SMEs within this industry display environmental responsibility. Market pull has a low incidence in encouraging ER, while values and the strategic decisions of entrepreneurs seem decisive. Policy makers should prioritize subsidies over fiscal incentives because they show greater potential to promote the adoption of environmental responsibility among these firms.
- Published
- 2017
- Full Text
- View/download PDF
33. Combining grazing incidence X-rays and micro-diffraction for qualitative phase identification in forensic powdered micro-samples
- Author
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Ministerio de Ciencia e Innovación (España), Sáez-Martínez, P., Gàrate-Lagos, J., Camargo, S., Torres-Roquer, F., Queralt Mitjans, Ignacio, Salazar-Kuri, U., Ministerio de Ciencia e Innovación (España), Sáez-Martínez, P., Gàrate-Lagos, J., Camargo, S., Torres-Roquer, F., Queralt Mitjans, Ignacio, and Salazar-Kuri, U.
- Abstract
Capillary diffraction or microdiffraction are standard techniques for characterizing small samples when only a few milligrams are available. On the other hand, we have typical grazing incidence diffraction or different variations of grazing incidence (GI) diffraction used, such as in-plane grazing incidence (IP-GI) or GI using a micro focusing source, to study thin films. However, when few powder micrograms are available, the characterization task is complicated. In the present work, few micrograms of typical forensic samples are analyzed using standard Bragg-Brentano, X-ray powder diffraction geometry, and grazing incidence X-ray micro-diffraction (GIµXRD). Samples include soils, cosmetic eyeshadows, two different pyrotechnic materials, and a highly explosive mixture contained at primer cup of ammunition. The analysis was carried out from 1 to 5 degrees of the incident parallel beam with a shaking sample. Depending on the fixed incident angle, different small regions of the diffraction patterns showed an improvement in the intensity of the peaks with respect to the conventional Bragg-Brentano configuration. However, 3–5 degrees of the fixed incident beam showed the best results. This new data acquisition technique, based on the combination of two known diffraction methods, could be a powerful tool for studying samples outside of forensic sciences such as nanomaterials, medicine, or any other field where the sample quantity is extremely small, also, without the need to transport evidence and travel to external facilities with higher analytical performance such as synchrotron radiation installations or other large experimental facilities.
- Published
- 2021
34. Rivalry and strategic groups: what makes a company a rival?
- Author
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González-Moreno, Ángela and Sáez-Martínez, Francisco J.
- Published
- 2008
- Full Text
- View/download PDF
35. Para un homenaje a Miguel Hernández (1910-2010) en E/LE: poesía y tecnologías educativas
- Author
-
Begoña Sáez Martínez
- Subjects
Education ,Philology. Linguistics ,P1-1091 - Abstract
La poesía siempre ha estado presente en la enseñanza de español para extranjeros. Unas veces como pretexto para enseñar lengua, otras como texto literario en sí mismo, muchas otras para ilustrar aspectos culturales. El problema es saber cómo poder integrarla en nuestros cursos mediante tareas motivadoras para los estudiantes. Una manera atractiva de incitar a los alumnos es formar parte de un proyecto global y real presentado como concurso.
- Published
- 2012
- Full Text
- View/download PDF
36. Para un homenaje a Miguel Hernández (1910-2010) en E/LE: poesía y tecnologías educativas
- Author
-
Begoña Sáez Martínez
- Subjects
Education ,Philology. Linguistics ,P1-1091 - Abstract
La poesía siempre ha estado presente en la enseñanza de español para extranjeros. Unas veces como pretexto para enseñar lengua, otras como texto literario en sí mismo, muchas otras para ilustrar aspectos culturales. El problema es saber cómo poder integrarla en nuestros cursos mediante tareas motivadoras para los estudiantes. Una manera atractiva de incitar a los alumnos es formar parte de un proyecto global y real presentado como concurso.
- Published
- 2011
37. Serum 25-OH vitamin D after bariatric surgery is correlated with changes in the molecular profile of inflammasome components
- Author
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Alcántara-Laguna, M., primary, León-Idougourram, S., additional, Herrero-Aguayo, V., additional, Sáez-Martínez, P., additional, Molina-Puertas, M., additional, Calañas-Continente, A., additional, Muñoz-Jiménez, C., additional, Membrives, A., additional, Gahete, M., additional, Luque, R., additional, Gálvez-Moreno, M., additional, and Herrera-Martínez, A., additional
- Published
- 2020
- Full Text
- View/download PDF
38. Pneumatosis cystoides, CT colonoscopy and endoscopic correlation
- Author
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Daniel Rodríguez-Sánchez, María Elena Sáez-Martínez, Regina María Sánchez-Jiménez, Juan de-Dios Berná-Mestre, and Florentina Guzmán-Aroca
- Subjects
Diseases of the digestive system. Gastroenterology ,RC799-869 - Published
- 2013
- Full Text
- View/download PDF
39. Evolución del paciente diagnosticado de sindrome respiratorio agudo severo por coronavirus tipo 2 en función de la comorbilidad de la población adscrita al Centro de Salud Zona VI de Albacete
- Author
-
Cantero-Quintero, Susana, Sáez-Martínez, Marta, and Castellanos-Garrido, Ana Belén
- Abstract
La bibliografía consultada, en su mayoría estudios descriptivos, establece asociación entre factores de riesgo para la salud, en su mayoría cardiovasculares, y la mala evolución de la COVID-19. Este estudio pretende mostrar dicha asociación entre la población perteneciente al área de salud zona vide Albacete (España)
- Published
- 2022
- Full Text
- View/download PDF
40. El manejo del asma como enfermedad inflamatoria crónica y problema sanitario global: documento de posicionamiento de las sociedades científicas
- Author
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Domínguez-Ortega, J., Sáez-Martínez, F.J., Gómez-Sáenz, J.T., Molina-París, J., Álvarez-Gutiérrez, F.J., Álvarez Gutiérrez, Francisco, Bárcena Caamaño, Mario, Blanco Aparicio, Marina, Delgado Romero, Julio, Domínguez Ortega, Javier, Ginel Mendoza, Leovigildo, Gómez Saenz, José Tomás, Hidalgo Requena, Antonio, Molina París, Jesús, Ocaña Rodríguez, Daniel, Plaza Moral, Vicente, Rodríguez Rodríguez, Mercedes, Román Rodríguez, Miguel, Sáez Martínez, Francisco José, and Trigueros Carrero, Juan Antonio
- Abstract
El asma es la enfermedad respiratoria más prevalente en el mundo; puede afectar a personas de todas las edades y es potencialmente mortal. En la actualidad, contamos con tratamientos de mantenimiento que son efectivos en la mayoría de los pacientes con asma y, sin embargo, una proporción importante no tiene bien controlada su enfermedad a pesar de los medios disponibles. En este documento, con el respaldo de las sociedades de los médicos que tratan el asma en España, se quiere llamar la atención de la sociedad y los profesionales sobre este problema en nuestro país. Se pone el foco sobre los aspectos clínicos, diagnósticos y terapéuticos del asma y se plantean algunas acciones de mejora en el ámbito de los pacientes y en el profesional sanitario que, en vista de los resultados actuales derivados de la falta de control del asma, podrían ser beneficiosas tanto en los resultados clínicos para los pacientes como en los de salud pública.
- Published
- 2020
- Full Text
- View/download PDF
41. Spliceosome components and splicing factors as novel therapeutic targets for prostate cancer
- Author
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Jiménez-Vacas, J.M., primary, Herrero-Aguayo, V., additional, Gómez-Gómez, E., additional, León-González, A.J., additional, Sáez-Martínez, P., additional, Montero-Hidalgo, A.J., additional, González-Serrano, T., additional, Requena-Tapia, M.J., additional, Castaño, J.P., additional, Gahete, M.D., additional, and Luque, R.M., additional
- Published
- 2019
- Full Text
- View/download PDF
42. Dysregulation of Components of the Inflammasome Machinery After Bariatric Surgery: Novel Targets for a Chronic Disease
- Author
-
Herrero-Aguayo, Vicente, Sáez-Martínez, Prudencio, López-Cánovas, Juan L, Prados-Carmona, Juan J, Alcántara-Laguna, María D, López, Fernando L, Molina-Puerta, María J, Calañas-Continente, Alfonso, Membrives, Antonio, Castilla, Juan, Ruiz-Ravelo, Juan, Alonso-Echague, Rosario, Yubero-Serrano, Elena M, Castaño, Justo P, Gahete, Manuel D, Gálvez-Moreno, María A, Luque, Raúl M, and Herrera-Martínez, Aura D
- Published
- 2021
- Full Text
- View/download PDF
43. Lirismos negros, novelas canallas: Las lobas de arrabal, por Antonio de Hoyos y Vinent
- Author
-
Sáez Martínez, Begoña
- Subjects
French literature - Italian literature - Spanish literature - Portuguese literature ,PQ1-3999 - Abstract
A partir de Las lobas de arrabal (1920) y su relación con el decadentismo se reflexiona sobre los cambios efectuados en la narrativa del cambio de siglo. La novela modernista se impregna de artificiosidad en busca de un efecto lírico, que a menudo se logra mediante la tensión entre lo bello y lo feo, lo sublime y lo canalla, lo elevado y lo abyecto. Surge de este modo un lirismo negro que constituye una pieza clave del modernismo español y que, sin embargo, en muchas ocasiones ha sido desatendido y hasta despreciado por el discurso crítico.
- Published
- 2010
- Full Text
- View/download PDF
44. Influence of Obesity in the miRNome: miR-4454, a Key Regulator of Insulin Response Via Splicing Modulation in Prostate
- Author
-
Herrero-Aguayo, Vicente, Jiménez-Vacas, Juan M, Sáez-Martínez, Prudencio, Gómez-Gómez, Enrique, López-Cánovas, Juan L, Garrido-Sánchez, Lourdes, Herrera-Martínez, Aura D, García-Bermejo, Laura, Macías-González, Manuel, López-Miranda, José, Castaño, Justo P, Gahete, Manuel D, and Luque, Raúl M
- Published
- 2021
- Full Text
- View/download PDF
45. Clinical, Cellular, and Molecular Evidence of the Additive Antitumor Effects of Biguanides and Statins in Prostate Cancer
- Author
-
Jiménez-Vacas, Juan M, Herrero-Aguayo, Vicente, Montero-Hidalgo, Antonio J, Sáez-Martínez, Prudencio, Gómez-Gómez, Enrique, León-González, Antonio J, Fuentes-Fayos, Antonio C, Yubero-Serrano, Elena M, Requena-Tapia, María J, López, Miguel, Castaño, Justo P, Gahete, Manuel D, and Luque, Raúl M
- Published
- 2021
- Full Text
- View/download PDF
46. Many or trusted partners for eco-innovation? The influence of breadth and depth of firms' knowledge network in the food sector.
- Author
-
González-Moreno, Ángela, Triguero, Ángela, and Sáez-Martínez, Francisco José
- Subjects
TECHNOLOGICAL innovations ,EMPIRICAL research ,FOOD industry ,STAKEHOLDERS ,INSTRUMENTAL variables (Statistics) - Abstract
Although literature on open eco-innovation has recently increased, empirical research on this topic in traditional low-tech sectors is still relatively scarce. The present paper examines how open innovation activities can promote eco-innovations in the food industry, an industry that is sometimes considered a technological laggard with traditionally low cooperation. The paper focuses on the relationship between a firm's interactions with stakeholders, particularly the breadth and the depth of the firm's knowledge network and the firm's propensity to develop different types of eco-innovations. Using a novel and more accurate measure of breadth and depth and addressing endogeneity with instrumental variables, the paper studies their influence on technological eco-innovation in a random sample of 279 food firms in Spain. The results show that coordination difficulties and bounded rationality explain an inverted U shape in the relationship of breadth of external knowledge sources and the propensity to eco-innovate both in product and process eco-innovations. Additionally, our findings confirm that it is important to rely on deep, frequent and intense relationships with stakeholders in order to create the required atmosphere to foster fluent knowledge sharing among partners specially to develop eco-process innovations, but a learning effect appears. Future research should extend the analysis to other countries and sectors to address the limitations of this study. • External search breadth is positively related to eco-innovation propensity. • Coordination with partners diminishes eco-innovation propensity. • Deep relationships improve knowledge flow among partners. • Deep, frequent and intense relationships foster eco-process innovations. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
47. Spliceosome component SF3B1 as novel prognostic biomarker and therapeutic target for prostate cancer.
- Author
-
Jiménez-Vacas, Juan M., Herrero-Aguayo, Vicente, Gómez-Gómez, Enrique, León-González, Antonio J., Sáez-Martínez, Prudencio, Alors-Pérez, Emilia, Fuentes-Fayos, Antonio C., Martínez-López, Ana, Sánchez-Sánchez, Rafael, González-Serrano, Teresa, López-Ruiz, Daniel J., Requena-Tapia, María J., Castaño, Justo P., Gahete, Manuel D., and Luque, Raúl M.
- Abstract
Prostate cancer (PCa) is one of the most common cancers types among men. Development and progression of PCa is associated with aberrant expression of oncogenic splicing-variants (eg, AR-v7), suggesting that dysregulation of the splicing process might represent a potential actionable target for PCa. Expression levels (mRNA and protein) of SF3B1, one of the main components of the splicing machinery, were analyzed in different cohorts of PCa patients (clinically localized [n = 84], highly aggressive PCa [n = 42], and TCGA dataset [n = 497]). Functional and mechanistic assays were performed in response to pladienolide-B in nontumor and tumor-derived prostate cells. Our results revealed that SF3B1 was overexpressed in PCa tissues and its levels were associated with clinically relevant PCa-aggressive features (eg, metastasis/AR-v7 expression). Moreover, inhibition of SF3B1 activity by pladienolide-B reduced functional parameters of aggressiveness (proliferation/migration/tumorspheres-formation/apoptosis) in PCa cell lines, irrespective of AR-v7 expression, and reduced viability of primary PCa cells. Antitumor actions of pladienolide-B involved: (1) inhibition of PI3K/AKT and JNK signaling pathways, (2) modulation of tumor markers and splicing variants (AR-v7/In1-ghrelin), and (3) regulation of key components of mRNA homeostasis-associated machineries (spliceosome/SURF/EJC). Altogether, our results demonstrated that SF3B1 is overexpressed and associated with malignant features in PCa, and its inhibition reduces PCa aggressiveness, suggesting that SF3B1 could represent a novel prognostic biomarker and a therapeutic target in PCa. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
48. 998 - Potential therapeutic role of the combination of biguanides and statins in prostate cancer: Association with alterations in key genes and miRNAs levels
- Author
-
Herrero-Aguayo, V., Jiménez-Vacas, J.M., Saéz-Martínez, P., Gómez-Gómez, E., León-González, A.J., Montero-Hidalgo, A.J., Requena-Tapia, M.J., Castaño, J.P., Gahete, M.D., and Luque, R.M.
- Published
- 2019
- Full Text
- View/download PDF
49. R&D team composition and product innovation: gender diversity makes a difference
- Author
-
González-Moreno, Ángela, Díaz-García, Cristina, and Sáez-Martínez, Francisco José
- Abstract
Literature presents conflicting views on the relationship between gender diversity and performance and we know very little about the impact of R&D team gender diversity on innovation. This study aims to fill this gap by drawing on social cognitive theory. In this paper, we investigate how innovation in R&D teams is fostered by a combination of the specific context of R&D tasks and the participation of mixed gender teams. Drawing on data from 3540 manufacturing firms, our results show that gender diversity has a non-linear impact on product innovation, supporting the argument that diversity is a 'double-edged sword'. This finding indicates that gender diversity fosters innovation only to a certain level. That is, moderate levels of gender diversity are more likely to be related to innovation, whereas lower or higher levels of gender diversity lead to less optimal results.
- Published
- 2018
- Full Text
- View/download PDF
50. OPEN AND GREEN INNOVATION IN THE HOSPITALITY INDUSTRY.
- Author
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Sáez-Martínez, Francisco José, Avellaneda-Rivera, Laura, and González-Moreno, Ángela
- Abstract
Hospitality is the main industry in the service sector in Spain, contributing to more than 10% of the gross domestic product and approximately 12% of all employees. Firms in this industry are beginning to adopt eco-innovations to reduce their environmental impact and optimize the use of natural resources. However, most companies do not have the necessary resources to tackle ecoinnovation on their own and need to cooperate with different agents. The purpose of this paper is to examine how open innovation activities can be a source of green innovation in the hospitality industry in Spain. This study contributes to the literature on both open innovation and eco-innovation by focusing on the relationship between a firm's green orientation and its interactions with stakeholders. We develop a model to assess how a firm's relationships with its clients, suppliers, competitors, and research institutions influence its environmental orientation. We propose that the depth and breadth of relationships with stakeholders positively influence the development of eco-innovations. We test our model with a sample of 189 firms from the Technological Innovation Panel (PITEC) database. The empirical analysis reveals that the benefits derived from these relationships follow an inverted U-shaped curve. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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