Search

Your search keyword '"Ryschich, Eduard"' showing total 264 results
Start Over Author "Ryschich, Eduard"
264 results on '"Ryschich, Eduard"'

3. In Situ Immunofluorescence Imaging of Vital Human Pancreatic Tissue Using Fiber‐Optic Microscopy.

Author

Ackermann, Sophia, Herold, Maximilian, Rohrbacher, Vincent, Schäfer, Michael, Tóth, Marcell, Thomann, Stefan, Hackert, Thilo, Ryschich, Eduard, and Rao, Tejeshwar

Subjects
EPITHELIAL cells, TISSUES, PHENOMENOLOGICAL biology, RESEARCH funding, XENOGRAFTS, FLUORESCENT antibody technique, BIOCHEMISTRY, MANN Whitney U Test, DESCRIPTIVE statistics, PANCREATIC tumors, FIBER optics, CELL culture, MICROSCOPY, DATA analysis software
Abstract

Purpose. Surgical resection is the only curative option for pancreatic carcinoma, but disease‐free and overall survival times after surgery are limited due to early tumor recurrence, most often originating from local microscopic tumor residues (R1 resection). The intraoperative identification of microscopic tumor residues within the resection margin in situ could improve surgical performance. The aim of this study was to evaluate the effectiveness of fiber‐optic microscopy for detecting microscopic residues in vital pancreatic cancer tissues. Experimental Design. Fresh whole‐mount human pancreatic tissues, histological tissue slides, cell culture, and chorioallantoic membrane xenografts were analyzed. Specimens were stained with selected fluorophore‐conjugated antibodies and studied using conventional wide‐field and self‐designed multicolor fiber‐optic fluorescence microscopy instruments. Results. Whole‐mount vital human tissues and xenografts were stained and imaged using an in situ immunofluorescence protocol. Fiber‐optic microscopy enabled the detection of epitope‐based fluorescence in vital whole‐mount tissue using fluorophore‐conjugated antibodies and enabled visualization of microvascular, epithelial, and malignant tumor cells. Among the selected antigen‐antibody pairs, antibody clones WM59, AY13, and 9C4 were the most promising for fiber‐optic imaging in human tissue samples and for endothelial, tumor and epithelial cell detection. Conclusions. Fresh dissected whole‐mount tissue can be stained using direct exposure to selected antibody clones. Several antibody clones were identified that provided excellent immunofluorescence imaging of labeled structures, such as endothelial, epithelial, or EGFR‐expressing cells. The combination of in situ immunofluorescence staining and fiber‐optic microscopy visualizes structures in vital tissues and could be proposed as an useful tool for the in situ identification of residual tumor mass in patients with a high operative risk for incomplete resection. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

6. Supplementary Figure S4 from YAP Orchestrates Heterotypic Endothelial Cell Communication via HGF/c-MET Signaling in Liver Tumorigenesis

Author

Thomann, Stefan, primary, Weiler, Sofia M.E., primary, Marquard, Simone, primary, Rose, Fabian, primary, Ball, Claudia R., primary, Tóth, Marcell, primary, Wei, Teng, primary, Sticht, Carsten, primary, Fritzsche, Sarah, primary, Roessler, Stephanie, primary, De La Torre, Carolina, primary, Ryschich, Eduard, primary, Ermakova, Olga, primary, Mogler, Carolin, primary, Kazdal, Daniel, primary, Gretz, Norbert, primary, Glimm, Hanno, primary, Rempel, Eugen, primary, Schirmacher, Peter, primary, and Breuhahn, Kai, primary

Published
2023
Full Text
View/download PDF

7. Supplementary Materials & Methods from YAP Orchestrates Heterotypic Endothelial Cell Communication via HGF/c-MET Signaling in Liver Tumorigenesis

Author

Thomann, Stefan, primary, Weiler, Sofia M.E., primary, Marquard, Simone, primary, Rose, Fabian, primary, Ball, Claudia R., primary, Tóth, Marcell, primary, Wei, Teng, primary, Sticht, Carsten, primary, Fritzsche, Sarah, primary, Roessler, Stephanie, primary, De La Torre, Carolina, primary, Ryschich, Eduard, primary, Ermakova, Olga, primary, Mogler, Carolin, primary, Kazdal, Daniel, primary, Gretz, Norbert, primary, Glimm, Hanno, primary, Rempel, Eugen, primary, Schirmacher, Peter, primary, and Breuhahn, Kai, primary

Published
2023
Full Text
View/download PDF

8. Supplementary Figure 3 from New Gene-Immunotherapy Combining TRAIL-Lymphocytes and EpCAMxCD3 Bispecific Antibody for Tumor Targeting

Author

Groth, Ariane, primary, Salnikov, Alexei V., primary, Ottinger, Sabine, primary, Gladkich, Jury, primary, Liu, Li, primary, Kallifatidis, Georgios, primary, Salnikova, Olga, primary, Ryschich, Eduard, primary, Giese, Nathalia, primary, Giese, Thomas, primary, Momburg, Frank, primary, Büchler, Markus W., primary, Moldenhauer, Gerhard, primary, and Herr, Ingrid, primary

Published
2023
Full Text
View/download PDF

9. Supplementary Methods, Figure 1 from New Gene-Immunotherapy Combining TRAIL-Lymphocytes and EpCAMxCD3 Bispecific Antibody for Tumor Targeting

Author

Groth, Ariane, primary, Salnikov, Alexei V., primary, Ottinger, Sabine, primary, Gladkich, Jury, primary, Liu, Li, primary, Kallifatidis, Georgios, primary, Salnikova, Olga, primary, Ryschich, Eduard, primary, Giese, Nathalia, primary, Giese, Thomas, primary, Momburg, Frank, primary, Büchler, Markus W., primary, Moldenhauer, Gerhard, primary, and Herr, Ingrid, primary

Published
2023
Full Text
View/download PDF

11. Supplementary Table 2 from New Gene-Immunotherapy Combining TRAIL-Lymphocytes and EpCAMxCD3 Bispecific Antibody for Tumor Targeting

Author

Groth, Ariane, primary, Salnikov, Alexei V., primary, Ottinger, Sabine, primary, Gladkich, Jury, primary, Liu, Li, primary, Kallifatidis, Georgios, primary, Salnikova, Olga, primary, Ryschich, Eduard, primary, Giese, Nathalia, primary, Giese, Thomas, primary, Momburg, Frank, primary, Büchler, Markus W., primary, Moldenhauer, Gerhard, primary, and Herr, Ingrid, primary

Published
2023
Full Text
View/download PDF

12. Supplementary Methods, Figure 2 from New Gene-Immunotherapy Combining TRAIL-Lymphocytes and EpCAMxCD3 Bispecific Antibody for Tumor Targeting

Author

Groth, Ariane, primary, Salnikov, Alexei V., primary, Ottinger, Sabine, primary, Gladkich, Jury, primary, Liu, Li, primary, Kallifatidis, Georgios, primary, Salnikova, Olga, primary, Ryschich, Eduard, primary, Giese, Nathalia, primary, Giese, Thomas, primary, Momburg, Frank, primary, Büchler, Markus W., primary, Moldenhauer, Gerhard, primary, and Herr, Ingrid, primary

Published
2023
Full Text
View/download PDF

13. Supplementary Table 1 from New Gene-Immunotherapy Combining TRAIL-Lymphocytes and EpCAMxCD3 Bispecific Antibody for Tumor Targeting

Author

Groth, Ariane, primary, Salnikov, Alexei V., primary, Ottinger, Sabine, primary, Gladkich, Jury, primary, Liu, Li, primary, Kallifatidis, Georgios, primary, Salnikova, Olga, primary, Ryschich, Eduard, primary, Giese, Nathalia, primary, Giese, Thomas, primary, Momburg, Frank, primary, Büchler, Markus W., primary, Moldenhauer, Gerhard, primary, and Herr, Ingrid, primary

Published
2023
Full Text
View/download PDF

14. Supplementary Figure Legends from YAP Orchestrates Heterotypic Endothelial Cell Communication via HGF/c-MET Signaling in Liver Tumorigenesis

Author

Thomann, Stefan, primary, Weiler, Sofia M.E., primary, Marquard, Simone, primary, Rose, Fabian, primary, Ball, Claudia R., primary, Tóth, Marcell, primary, Wei, Teng, primary, Sticht, Carsten, primary, Fritzsche, Sarah, primary, Roessler, Stephanie, primary, De La Torre, Carolina, primary, Ryschich, Eduard, primary, Ermakova, Olga, primary, Mogler, Carolin, primary, Kazdal, Daniel, primary, Gretz, Norbert, primary, Glimm, Hanno, primary, Rempel, Eugen, primary, Schirmacher, Peter, primary, and Breuhahn, Kai, primary

Published
2023
Full Text
View/download PDF

17. YAP‐induced Ccl2 expression is associated with a switch in hepatic macrophage identity and vascular remodelling in liver cancer

Author

Thomann, Stefan, primary, Weiler, Sofia M. E., additional, Wei, Teng, additional, Sticht, Carsten, additional, De La Torre, Carolina, additional, Tóth, Marcell, additional, Rose, Fabian, additional, Tang, Yingyue, additional, Ritz, Thomas, additional, Ball, Claudia, additional, Glimm, Hanno, additional, Ryschich, Eduard, additional, Schirmacher, Peter, additional, and Breuhahn, Kai, additional

Published
2021
Full Text
View/download PDF

22. Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery

Author

Qian, Baifeng, Kyuno, Daisuke, Schäfer, Michael, Gross, Wolfgang, Mehrabi, Arianeb, and Ryschich, Eduard

Subjects
IRDye 800CW, Indoles, Staining and Labeling, Benzenesulfonates, Liver Neoplasms, Intraoperative navigation, intravascular sequestration, Intercellular Adhesion Molecule-1, Monocytes, Mice, Inbred C57BL, Mice, Liver, Microvessels, Animals, Hepatectomy, Humans, Research Paper, Fluorescent Dyes
Abstract

Background: The accurate determination of liver segment anatomy is essential to perform liver resection without complications and to ensure long-term outcomes after this operation. There are several perioperative methods for segment identification and surgical navigation, such as intraoperative ultrasound, indigo carmine and near-infrared imaging with indocyanine green. The present study experimentally analyzed the usefulness of monocyte sequestration for liver segment labeling and imaging. Methods: Human monocytes were isolated from peripheral blood and directly or indirectly labeled with calcein or IRDye 800CW. Potential toxicity, labeling stability, and adhesion to ICAM-1 were analyzed in vitro. Monocyte sequestration in the liver microvasculature and liver segment labeling and boundary demarcation were studied using isolated mouse and pig liver perfusion and via intraportal injection in mouse liver tumor models. Results: The highest monocyte labeling efficiency was achieved using direct labeling with IRDye 800CW. Labeling was stable and did not influence cell viability. The labeled monocytes were highly sequestrated in the liver microvasculature, both after ex vivo perfusion and after injection in vivo , resulting in excellent labeling of selected liver segments and strong segment boundary demarcation. In contrast to results to a normal liver, monocyte sequestration was very low in tumor-associated blood vessels. Conclusions: The present experimental study shows that sequestration of labeled monocytes after superselective application demarcates the selected liver segment. These results illustrate potential of this technique for surgical navigation during liver surgery.

Published
2018

25. YAP Orchestrates Heterotypic Endothelial Cell Communication via HGF/c-MET Signaling in Liver Tumorigenesis

Author

Thomann, Stefan, primary, Weiler, Sofia M.E., additional, Marquard, Simone, additional, Rose, Fabian, additional, Ball, Claudia R., additional, Tóth, Marcell, additional, Wei, Teng, additional, Sticht, Carsten, additional, Fritzsche, Sarah, additional, Roessler, Stephanie, additional, De La Torre, Carolina, additional, Ryschich, Eduard, additional, Ermakova, Olga, additional, Mogler, Carolin, additional, Kazdal, Daniel, additional, Gretz, Norbert, additional, Glimm, Hanno, additional, Rempel, Eugen, additional, Schirmacher, Peter, additional, and Breuhahn, Kai, additional

Published
2020
Full Text
View/download PDF

40. PS-041-Intense angiocrine crosstalk between liver endothelial cell subtypes leads to liver capillarization and recruitment of myeloid progenitors

Author

Thomann, Stefan, primary, Weiler, Sofia, additional, Marquard, Simone, additional, Dittmer, Martin, additional, Kazdal, Daniel, additional, Tóth, Marcell, additional, Wei, Teng, additional, Roessler, Stephanie, additional, Sticht, Carsten, additional, Gretz, Norbert, additional, Mogler, Carolin, additional, Ball, Claudia, additional, Glimm, Hanno, additional, Ryschich, Eduard, additional, Schirmacher, Peter, additional, and Breuhahn, Kai, additional

Published
2019
Full Text
View/download PDF

43. Blockade of leukocyte haptokinesis and haptotaxis by ketoprofen, diclofenac and SC-560

Author

Paskauskas Saulius, Parseliunas Audrius, Kerkadze Vachtang, Nobiling Rainer, Schmidt Jan, and Ryschich Eduard

Subjects
Immunologic diseases. Allergy, RC581-607
Abstract

Abstract Background Nonsteroidal anti-inflammatory drugs (NSAID) represent a one of the most widely used anti-inflammatory substances. Their anti-inflammatory effects are mainly based on inhibition of cyclooxygenase. The potential direct effect of NSAID on leukocyte migration was poorly investigated. Using time-lapse microscopy and 96-well fluorescence-based assay, we studied the effect of three different NSAID, ketoprofen, diclofenac and SC-560, on leukocyte haptokinesis and haptotaxis in vivo and in vitro. Results NSAID induced an immediate inhibiting effect on leukocyte migration both in vitro and in vivo. This effect was dose-dependent and was not restricted to a specific type of leukocytes. The inhibition of leukocyte migration by NSAID was partially re-stored after removal of inhibiting agent. Only complete blockade of leukocyte migration was accompanied by a strong reduction of [Ca2+]i. Conclusions NSAID strongly supress leukocyte migration. The results of the present study may have important clinical implications since blockade of leukocyte migration can be achieved after topical application of NSAID.

Published
2011
Full Text
View/download PDF

45. Isovolemic hemodilution with dextran prevents contrast medium induced impairment of pancreatic microcirculation in necrotizing pancreatitis of the rat

Author

Hotz, Hubert G., Schmidt, Jan, Ryschich, Eduard W., Foitzik, Thomas, Buhr, Heinz J., Warshaw, Andrew L., Herfarth, Christian, and Klar, Ernst

Subjects
Dextran -- Usage, Contrast media -- Physiological aspects, Pancreatitis -- Complications, Microcirculation -- Physiological aspects, Health
Abstract

BACKGROUND: Previous studies demonstrated that intravenous contrast medium (CM), as used in contrast enhanced computed tomography, aggravates the impairment of pancreatic microcirculation (PM) characteristic of severe pancreatitis and increases necrosis and mortality in necrotizing pancreatitis (NP) in rats. This study evaluates the use of isovolemic hemodilution, which can enhance the microcirculation in severe pancreatitis, for preventing CM-induced injury. METHODS: NP was induced in 30 dextran-tolerant Wistar rats by intraductal glycodeoxycholic acid and intravenous cerulin for 6 hours. PM was quantified by intravital microscopy using fluorescein isothiocyanate labeled erythrocytes. Based on previous results, areas with low blood flow (

Published
1995

47. Abstract 2050: Tumor stage-dependent transcriptomic signatures of endothelial cells reveal an intense paracrine crosstalk in liver capillarization

Author

Thomann, Stefan, primary, Weiler, Sofia, additional, Dittmer, Martin, additional, Kazdal, Daniel, additional, Marquard, Simone, additional, Roessler, Stephanie, additional, Sticht, Carsten, additional, Gretz, Norbert, additional, Mogler, Carolin, additional, Ball, Claudia, additional, Glimm, Hanno, additional, Ryschich, Eduard, additional, Schirmacher, Peter, additional, and Breuhahn, Kai, additional

Published
2018
Full Text
View/download PDF

50. Aspirin counteracts cancer stem cell features, desmoplasia and gemcitabine resistance in pancreatic cancer

Author

Zhang, Yiyao, primary, Liu, Li, additional, Fan, Pei, additional, Bauer, Nathalie, additional, Gladkich, Jury, additional, Ryschich, Eduard, additional, Bazhin, Alexandr V., additional, Giese, Nathalia A., additional, Strobel, Oliver, additional, Hackert, Thilo, additional, Hinz, Ulf, additional, Gross, Wolfgang, additional, Fortunato, Franco, additional, and Herr, Ingrid, additional

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results