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2 results on '"Ryosuke Kohara"'

1. The non-synonymous mutation in bovine SPP1 gene influences carcass weight

Author

Hirokazu Matsumoto, Ryosuke Kohara, Makoto Sugi, Azumi Usui, Kenji Oyama, Hideyuki Mannen, and Shinji Sasazaki

Subjects
Genetics, Animal breeding, Cattle, DNA sequencing, Gene mutation, Promoter, Science (General), Q1-390, Social sciences (General), H1-99
Abstract

Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.

Published
2019
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2. The non-synonymous mutation in bovine SPP1 gene influences carcass weight

Author

Kenji Oyama, Ryosuke Kohara, Makoto Sugi, Hideyuki Mannen, Hirokazu Matsumoto, Shinji Sasazaki, and Azumi Usui

Subjects
0301 basic medicine, Candidate gene, Single-nucleotide polymorphism, Beef cattle, Biology, Gene mutation, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, medicine, Genetics, Coding region, DNA sequencing, Allele, lcsh:Social sciences (General), lcsh:Science (General), Gene, Animal breeding, Mutation, Multidisciplinary, food and beverages, Promoter, 030104 developmental biology, Cattle, lcsh:H1-99, 030217 neurology & neurosurgery, lcsh:Q1-390
Abstract

Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.

Published
2019

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