73 results on '"Ryazanov AG"'
Search Results
2. PWE-021 Mtorc1 Mediated Translational Elongation Is Limiting For Intestinal Tumour Initiation And Growth
- Author
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Faller, WJ, primary, Jackson, TJ, additional, Ridgway, RA, additional, Knight, JR, additional, Jamieson, T, additional, Pende, M, additional, Ryazanov, AG, additional, Sonenberg, N, additional, Meyuhas, O, additional, Bushell, M, additional, Hall, MN, additional, Willis, AE, additional, and Sansom, OJ, additional
- Published
- 2014
- Full Text
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3. Suppression of eEF2 phosphorylation alleviates synaptic failure and cognitive deficits in mouse models of Down syndrome.
- Author
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Wang X, Yang Q, Zhou X, Keene CD, Ryazanov AG, and Ma T
- Subjects
- Animals, Mice, Phosphorylation, Humans, Male, Brain metabolism, Synapses metabolism, Synapses pathology, Female, Mice, Transgenic, Down Syndrome metabolism, Down Syndrome pathology, Disease Models, Animal, Elongation Factor 2 Kinase metabolism, Elongation Factor 2 Kinase genetics, Cognitive Dysfunction metabolism, Peptide Elongation Factor 2 metabolism
- Abstract
Introduction: Cognitive impairment is a core feature of Down syndrome (DS), and the underlying neurobiological mechanisms remain unclear. Translation dysregulation is linked to multiple neurological disorders characterized by cognitive impairments. Phosphorylation of the translational factor eukaryotic elongation factor 2 (eEF2) by its kinase eEF2K results in inhibition of general protein synthesis., Methods: We used genetic and pharmacological methods to suppress eEF2K in two lines of DS mouse models. We further applied multiple approaches to evaluate the effects of eEF2K inhibition on DS pathophysiology., Results: We found that eEF2K signaling was overactive in the brain of patients with DS and DS mouse models. Inhibition of eEF2 phosphorylation through suppression of eEF2K in DS model mice improved multiple aspects of DS-associated pathophysiology including de novo protein synthesis deficiency, synaptic morphological defects, long-term synaptic plasticity failure, and cognitive impairments., Discussion: Our data suggested that eEF2K signaling dysregulation mediates DS-associated synaptic and cognitive impairments., Highlights: Phosphorylation of the translational factor eukaryotic elongation factor 2 (eEF2) is increased in the Down syndrome (DS) brain. Suppression of the eEF2 kinase (eEF2K) alleviates cognitive deficits in DS models. Suppression of eEF2K improves synaptic dysregulation in DS models. Cognitive and synaptic impairments in DS models are rescued by eEF2K inhibitors., (© 2024 The Author(s). Alzheimer's & Dementia published by Wiley Periodicals LLC on behalf of Alzheimer's Association.)
- Published
- 2024
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4. Transcriptional-translational conflict is a barrier to cellular transformation and cancer progression.
- Author
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Jana S, Brahma S, Arora S, Wladyka CL, Hoang P, Blinka S, Hough R, Horn JL, Liu Y, Wang LJ, Depeille P, Smith E, Montgomery RB, Lee JK, Haffner MC, Vakar-Lopez F, Grivas P, Wright JL, Lam HM, Black PC, Roose JP, Ryazanov AG, Subramaniam AR, Henikoff S, and Hsieh AC
- Subjects
- Humans, Chromatin, Urinary Bladder Neoplasms genetics
- Abstract
We uncover a tumor-suppressive process in urothelium called transcriptional-translational conflict caused by deregulation of the central chromatin remodeling component ARID1A. Loss of Arid1a triggers an increase in a nexus of pro-proliferation transcripts, but a simultaneous inhibition of the eukaryotic elongation factor 2 (eEF2), which results in tumor suppression. Resolution of this conflict through enhancing translation elongation speed enables the efficient and precise synthesis of a network of poised mRNAs resulting in uncontrolled proliferation, clonogenic growth, and bladder cancer progression. We observe a similar phenomenon in patients with ARID1A-low tumors, which also exhibit increased translation elongation activity through eEF2. These findings have important clinical implications because ARID1A-deficient, but not ARID1A-proficient, tumors are sensitive to pharmacologic inhibition of protein synthesis. These discoveries reveal an oncogenic stress created by transcriptional-translational conflict and provide a unified gene expression model that unveils the importance of the crosstalk between transcription and translation in promoting cancer., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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5. An atlas of substrate specificities for the human serine/threonine kinome.
- Author
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Johnson JL, Yaron TM, Huntsman EM, Kerelsky A, Song J, Regev A, Lin TY, Liberatore K, Cizin DM, Cohen BM, Vasan N, Ma Y, Krismer K, Robles JT, van de Kooij B, van Vlimmeren AE, Andrée-Busch N, Käufer NF, Dorovkov MV, Ryazanov AG, Takagi Y, Kastenhuber ER, Goncalves MD, Hopkins BD, Elemento O, Taatjes DJ, Maucuer A, Yamashita A, Degterev A, Uduman M, Lu J, Landry SD, Zhang B, Cossentino I, Linding R, Blenis J, Hornbeck PV, Turk BE, Yaffe MB, and Cantley LC
- Subjects
- Humans, Phosphorylation, Substrate Specificity, Datasets as Topic, Cell Line, Phosphoserine metabolism, Phosphothreonine metabolism, Protein Serine-Threonine Kinases metabolism, Serine metabolism, Threonine metabolism, Proteome chemistry, Proteome metabolism, Phosphoproteins chemistry, Phosphoproteins metabolism
- Abstract
Protein phosphorylation is one of the most widespread post-translational modifications in biology
1,2 . With advances in mass-spectrometry-based phosphoproteomics, 90,000 sites of serine and threonine phosphorylation have so far been identified, and several thousand have been associated with human diseases and biological processes3,4 . For the vast majority of phosphorylation events, it is not yet known which of the more than 300 protein serine/threonine (Ser/Thr) kinases encoded in the human genome are responsible3 . Here we used synthetic peptide libraries to profile the substrate sequence specificity of 303 Ser/Thr kinases, comprising more than 84% of those predicted to be active in humans. Viewed in its entirety, the substrate specificity of the kinome was substantially more diverse than expected and was driven extensively by negative selectivity. We used our kinome-wide dataset to computationally annotate and identify the kinases capable of phosphorylating every reported phosphorylation site in the human Ser/Thr phosphoproteome. For the small minority of phosphosites for which the putative protein kinases involved have been previously reported, our predictions were in excellent agreement. When this approach was applied to examine the signalling response of tissues and cell lines to hormones, growth factors, targeted inhibitors and environmental or genetic perturbations, it revealed unexpected insights into pathway complexity and compensation. Overall, these studies reveal the intrinsic substrate specificity of the human Ser/Thr kinome, illuminate cellular signalling responses and provide a resource to link phosphorylation events to biological pathways., (© 2023. The Author(s).)- Published
- 2023
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6. Homozygous knockout of eEF2K alleviates cognitive deficits in APP/PS1 Alzheimer's disease model mice independent of brain amyloid β pathology.
- Author
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Kasica NP, Zhou X, Jester HM, Holland CE, Ryazanov AG, Forshaw TE, Furdui CM, and Ma T
- Abstract
Maintenance of memory and synaptic plasticity depends on de novo protein synthesis, and accumulating evidence implicates a role of dysregulated mRNA translation in cognitive impairments associated with Alzheimer's disease (AD). Accumulating evidence demonstrates hyper-phosphorylation of translation factor eukaryotic elongation factor 2 (eEF2) in the hippocampi of human AD patients as well as transgenic AD model mice. Phosphorylation of eEF2 (at the Thr 56 site) by its only known kinase, eEF2K, leads to inhibition of general protein synthesis. A recent study suggests that amyloid β (Aβ)-induced neurotoxicity could be associated with an interaction between eEF2 phosphorylation and the transcription factor nuclear erythroid 2-related factor (NRF2)-mediated antioxidant response. In this brief communication, we report that global homozygous knockout of the eEF2K gene alleviates deficits of long-term recognition and spatial learning in a mouse model of AD (APP/PS1). Moreover, eEF2K knockout does not alter brain Aβ pathology in APP/PS1 mice. The hippocampal NRF2 antioxidant response in the APP/PS1 mice, measured by expression levels of nicotinamide adenine dinucleotide plus hydrogen (NADPH) quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1), is ameliorated by suppression of eEF2K signaling. Together, the findings may contribute to our understanding of the molecular mechanisms underlying AD pathogenesis, indicating that suppression of eEF2K activity could be a beneficial therapeutic option for this devastating neurodegenerative disease., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Kasica, Zhou, Jester, Holland, Ryazanov, Forshaw, Furdui and Ma.)
- Published
- 2022
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7. TRPM7 deficiency exacerbates cardiovascular and renal damage induced by aldosterone-salt.
- Author
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Rios FJ, Zou ZG, Harvey AP, Harvey KY, Camargo LL, Neves KB, Nichol SEF, Alves-Lopes R, Cheah A, Zahraa M, Ryazanov AG, Ryazanova L, Gudermann T, Chubanov V, Montezano AC, and Touyz RM
- Subjects
- Aldosterone pharmacology, Animals, Fibrosis, Kidney metabolism, Magnesium metabolism, Mice, Protein Phosphatase 2C metabolism, Sodium Chloride, Hyperaldosteronism genetics, Hyperaldosteronism metabolism, TRPM Cation Channels deficiency, TRPM Cation Channels genetics, TRPM Cation Channels metabolism
- Abstract
Hyperaldosteronism causes cardiovascular disease as well as hypomagnesemia. Mechanisms are ill-defined but dysregulation of TRPM7, a Mg
2+ -permeable channel/α-kinase, may be important. We examined the role of TRPM7 in aldosterone-dependent cardiovascular and renal injury by studying aldosterone-salt treated TRPM7-deficient (TRPM7+/Δkinase ) mice. Plasma/tissue [Mg2+ ] and TRPM7 phosphorylation were reduced in vehicle-treated TRPM7+/Δkinase mice, effects recapitulated in aldosterone-salt-treated wild-type mice. Aldosterone-salt treatment exaggerated vascular dysfunction and amplified cardiovascular and renal fibrosis, with associated increased blood pressure in TRPM7+/Δkinase mice. Tissue expression of Mg2+ -regulated phosphatases (PPM1A, PTEN) was downregulated and phosphorylation of Smad3, ERK1/2, and Stat1 was upregulated in aldosterone-salt TRPM7-deficient mice. Aldosterone-induced phosphorylation of pro-fibrotic signaling was increased in TRPM7+/Δkinase fibroblasts, effects ameliorated by Mg2+ supplementation. TRPM7 deficiency amplifies aldosterone-salt-induced cardiovascular remodeling and damage. We identify TRPM7 downregulation and associated hypomagnesemia as putative molecular mechanisms underlying deleterious cardiovascular and renal effects of hyperaldosteronism., (© 2022. The Author(s).)- Published
- 2022
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8. Agonists in the Extended Conformation Stabilize the Active State of β-Adrenoceptors.
- Author
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Efimov AV, Meshcheryakova OV, and Ryazanov AG
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- Models, Molecular, Nitrogen, Oxygen, Protein Conformation, Protein Structure, Secondary, Receptors, Adrenergic, Adrenergic beta-Agonists, Carbon
- Abstract
In this study, we conducted a comparative analysis of the structure of agonists and antagonists of transmembrane (TM) β-adrenoceptors (β-ARs) and their interactions with the β-ARs and proposed the mechanism of receptor activation. A characteristic feature of agonist and antagonist molecules is the presence of a hydrophobic head (most often, one or two aromatic rings) and a tail with a positively charged amino group. All β-adrenergic agonists have two carbon atoms between the aromatic ring of the head and the nitrogen atom of the amino group. In antagonist molecules, this fragment can be either reduced or increased to four atoms due to the additional carbon and oxygen atoms. The agonist head, as a rule, has two H-bond donors or acceptors in the para- and meta-positions of the aromatic rings, while in the antagonist heads, these donors/acceptors are absent or located in other positions. Analysis of known three-dimensional structures of β-AR complexes with agonists showed that the agonist head forms two H-bonds with the TM5 helix, and the tail forms an ionic bond with the D3.32 residue of the TM3 helix and one or two H-bonds with the TM7 helix. The tail of the antagonist can form similar bonds, but the interaction between the head and the TM5 helix is much weaker. As a result of these interactions, the agonist molecule acquires an extended "strained string" conformation, in contrast to the antagonist molecule, which has a longer, bended, and flexible tail. The "strained string" of the agonist interacts with the TM6 helix (primarily with the W6.48 residue) and turns it, which leads to the opening of the G protein-binding site on the intracellular side of the receptor, while flexible and larger antagonist molecules do not have the same effect on the receptor.
- Published
- 2022
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9. Antagonists targeting eEF2 kinase rescue multiple aspects of pathophysiology in Alzheimer's disease model mice.
- Author
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Kasica NP, Zhou X, Yang Q, Wang X, Yang W, Zimmermann HR, Holland CE, Koscielniak E, Wu H, Cox AO, Lee J, Ryazanov AG, Furdui CM, and Ma T
- Subjects
- Amyloid beta-Peptides metabolism, Animals, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase metabolism, Mice, Peptide Elongation Factor 2 metabolism, Phosphorylation, Syndrome, Alzheimer Disease metabolism
- Abstract
It is imperative to develop novel therapeutic strategies for Alzheimer's disease (AD) and related dementia syndromes based on solid mechanistic studies. Maintenance of memory and synaptic plasticity relies on de novo protein synthesis, which is partially regulated by phosphorylation of eukaryotic elongation factor 2 (eEF2) via its kinase eEF2K. Abnormally increased eEF2 phosphorylation and impaired mRNA translation have been linked to AD. We recently reported that prenatal genetic suppression of eEF2K is able to prevent aging-related cognitive deficits in AD model mice, suggesting the therapeutic potential of targeting eEF2K/eEF2 signaling in AD. Here, we tested two structurally distinct small-molecule eEF2K inhibitors in two different lines of AD model mice after the onset of cognitive impairments. Our data revealed that treatment with eEF2K inhibitors improved AD-associated synaptic plasticity impairments and cognitive dysfunction, without altering brain amyloid β (Aβ) and tau pathology. Furthermore, eEF2K inhibition alleviated AD-associated defects in dendritic spine morphology, post-synaptic density formation, protein synthesis, and dendritic polyribosome assembly. Our results may offer critical therapeutic implications for AD, and the proof-of-principle study indicates translational implication of inhibiting eEF2K for AD and related dementia syndromes. Cover Image for this issue: https://doi.org/10.1111/jnc.15392., (© 2021 International Society for Neurochemistry.)
- Published
- 2022
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10. Suppression of the kinase for elongation factor 2 alleviates mGluR-LTD impairments in a mouse model of Alzheimer's disease.
- Author
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Yang W, Zhou X, Ryazanov AG, and Ma T
- Subjects
- Alzheimer Disease genetics, Animals, Disease Models, Animal, Hippocampus metabolism, Imidazoles pharmacology, Mice, Transgenic, Neuronal Plasticity genetics, Peptide Elongation Factor 2 antagonists & inhibitors, Phosphorylation, Protein Biosynthesis, Receptor, Metabotropic Glutamate 5 metabolism, Alzheimer Disease etiology, Long-Term Potentiation genetics, Long-Term Potentiation physiology, Peptide Elongation Factor 2 genetics, Peptide Elongation Factor 2 metabolism, Receptor, Metabotropic Glutamate 5 genetics, Receptor, Metabotropic Glutamate 5 physiology
- Abstract
Impaired mRNA translation (protein synthesis) is linked to Alzheimer's disease (AD) pathophysiology. Recent studies revealed the role of increased phosphorylation of eukaryotic elongation factor 2 (eEF2) in AD-associated cognitive deficits. Phosphorylation of eEF2 (at the Thr56 site) by its only known kinase eEF2K leads to inhibition of general protein synthesis. AD is considered as a disease of "synaptic failure" characterized by impairments of synaptic plasticity, including long-term potentiation (LTP) and long-term depression (LTD). Deficiency of metabotropic glutamate receptor 5-dependent LTD (mGluR-LTD) is indicated in cognitive syndromes associated with various neurological disorders, including AD, but the molecular signaling mechanisms underlying the mGluR-LTD dysregulation in AD remain unclear. In this brief communication, we report genetic repression of eEF2K in aged APP/PS1 AD model mice prevented AD-associated hippocampal mGluR-LTD deficits. Using a pharmacological approach, we further observed that impairments of mGluR-LTD in APP/PS1 mice were rescued by treating hippocampal slices with a small molecule eEF2K antagonist NH125. Our findings, taken together, suggest a critical role of abnormal protein synthesis dysregulation at the elongation phase in AD-associated mGluR-LTD failure, thus providing insights into a mechanistic understanding of synaptic impairments in AD and other related dementia syndromes., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2021
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11. Repression of eEF2 kinase improves deficits in novel object recognition memory in aged mice.
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Gosrani SP, Jester HM, Zhou X, Ryazanov AG, and Ma T
- Subjects
- Aging pathology, Animals, Cognitive Dysfunction psychology, Disease Models, Animal, Female, Hippocampus metabolism, Hippocampus pathology, Male, Mice, Knockout, Phosphorylation, Aging psychology, Cognitive Aging, Cognitive Dysfunction etiology, Cognitive Dysfunction prevention & control, Elongation Factor 2 Kinase metabolism, Elongation Factor 2 Kinase physiology, Memory, Recognition, Psychology
- Abstract
The normal aging process is commonly associated with mild cognitive deficits including memory decline. Previous studies indicate a role of dysregulated messenger ribonucleic acid translation capacity in cognitive defects associated with aging and aging-related diseases, including hyperphosphorylation of eukaryotic elongation factor 2 (eEF2). Phosphorylation of eEF2 by the kinase eEF2K inhibits its activity, hindering general protein synthesis. Here, we sought to determine whether cognitive deficits in aged mice can be improved by genetically deleting eEF2K (eEF2K KO) and consequently reduction of eEF2 phosphorylation. We found that suppression of eEF2K prevented aging-related deficits in novel object recognition memory. Interestingly, deletion of eEF2K did not alter overall protein synthesis in the hippocampus. Ultrastructural analysis revealed increase size and larger active zone lengths of postsynaptic densities in the hippocampus of aged eEF2K KO mice. Biochemical assays showed hippocampal eIF2α hyperphosphorylation in aged eEF2K KO mice, indicating inhibition of translation initiation. Our findings may provide insight into mechanistic understanding and thus development of novel therapeutic strategies for aging-related cognitive decline., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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12. Chanzyme TRPM7 protects against cardiovascular inflammation and fibrosis.
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Rios FJ, Zou ZG, Harvey AP, Harvey KY, Nosalski R, Anyfanti P, Camargo LL, Lacchini S, Ryazanov AG, Ryazanova L, McGrath S, Guzik TJ, Goodyear CS, Montezano AC, and Touyz RM
- Subjects
- Animals, Cardiomegaly genetics, Cardiomegaly pathology, Cardiomegaly physiopathology, Cardiomyopathies genetics, Cardiomyopathies pathology, Cardiomyopathies physiopathology, Cell Proliferation, Cells, Cultured, Coculture Techniques, Fibroblasts metabolism, Fibroblasts pathology, Fibrosis, Inflammation genetics, Inflammation pathology, Inflammation physiopathology, Leukocyte Rolling, Macrophages metabolism, Macrophages pathology, Magnesium metabolism, Male, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Myocardium pathology, Signal Transduction, TRPM Cation Channels deficiency, TRPM Cation Channels genetics, Transendothelial and Transepithelial Migration, Cardiomegaly metabolism, Cardiomyopathies metabolism, Inflammation metabolism, Inflammation Mediators metabolism, Myocardium metabolism, TRPM Cation Channels metabolism, Ventricular Remodeling
- Abstract
Aims: Transient Receptor Potential Melastatin 7 (TRPM7) cation channel is a chanzyme (channel + kinase) that influences cellular Mg2+ homeostasis and vascular signalling. However, the pathophysiological significance of TRPM7 in the cardiovascular system is unclear. The aim of this study was to investigate the role of this chanzyme in the cardiovascular system focusing on inflammation and fibrosis., Methods and Results: TRPM7-deficient mice with deletion of the kinase domain (TRPM7+/Δkinase) were studied and molecular mechanisms investigated in TRPM7+/Δkinase bone marrow-derived macrophages (BMDM) and co-culture systems with cardiac fibroblasts. TRPM7-deficient mice had significant cardiac hypertrophy, fibrosis, and inflammation. Cardiac collagen and fibronectin content, expression of pro-inflammatory mediators (SMAD3, TGFβ) and cytokines [interleukin (IL)-6, IL-10, IL-12, tumour necrosis factor-α] and phosphorylation of the pro-inflammatory signalling molecule Stat1, were increased in TRPM7+/Δkinase mice. These processes were associated with infiltration of inflammatory cells (F4/80+CD206+ cardiac macrophages) and increased galectin-3 expression. Cardiac [Mg2+]i, but not [Ca2+]i, was reduced in TRPM7+/Δkinase mice. Calpain, a downstream TRPM7 target, was upregulated (increased expression and activation) in TRPM7+/Δkinase hearts. Vascular functional and inflammatory responses, assessed in vivo by intra-vital microscopy, demonstrated impaired neutrophil rolling, increased neutrophil: endothelial attachment and transmigration of leucocytes in TRPM7+/Δkinase mice. TRPM7+/Δkinase BMDMs had increased levels of galectin-3, IL-10, and IL-6. In co-culture systems, TRPM7+/Δkinase macrophages increased expression of fibronectin, proliferating cell nuclear antigen, and TGFβ in cardiac fibroblasts from wild-type mice, effects ameliorated by MgCl2 treatment., Conclusions: We identify a novel anti-inflammatory and anti-fibrotic role for TRPM7 and suggest that its protective effects are mediated, in part, through Mg2+-sensitive processes., (© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Cardiology.)
- Published
- 2020
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13. Glycine supplementation extends lifespan of male and female mice.
- Author
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Miller RA, Harrison DE, Astle CM, Bogue MA, Brind J, Fernandez E, Flurkey K, Javors M, Ladiges W, Leeuwenburgh C, Macchiarini F, Nelson J, Ryazanov AG, Snyder J, Stearns TM, Vaughan DE, and Strong R
- Subjects
- Adenomatosis, Pulmonary epidemiology, Aging drug effects, Animals, Aspirin pharmacology, Diet, Female, Inulin pharmacology, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Piperazines pharmacology, para-Aminobenzoates pharmacology, Aging metabolism, Dietary Supplements, Glycine pharmacology, Longevity drug effects
- Abstract
Diets low in methionine extend lifespan of rodents, though through unknown mechanisms. Glycine can mitigate methionine toxicity, and a small prior study has suggested that supplemental glycine could extend lifespan of Fischer 344 rats. We therefore evaluated the effects of an 8% glycine diet on lifespan and pathology of genetically heterogeneous mice in the context of the Interventions Testing Program. Elevated glycine led to a small (4%-6%) but statistically significant lifespan increase, as well as an increase in maximum lifespan, in both males (p = 0.002) and females (p < 0.001). Pooling across sex, glycine increased lifespan at each of the three independent sites, with significance at p = 0.01, 0.053, and 0.03, respectively. Glycine-supplemented females were lighter than controls, but there was no effect on weight in males. End-of-life necropsies suggested that glycine-treated mice were less likely than controls to die of pulmonary adenocarcinoma (p = 0.03). Of the 40 varieties of incidental pathology evaluated in these mice, none were increased to a significant degree by the glycine-supplemented diet. In parallel analyses of the same cohort, we found no benefits from TM5441 (an inhibitor of PAI-1, the primary inhibitor of tissue and urokinase plasminogen activators), inulin (a source of soluble fiber), or aspirin at either of two doses. Our glycine results strengthen the idea that modulation of dietary amino acid levels can increase healthy lifespan in mice, and provide a foundation for further investigation of dietary effects on aging and late-life diseases., (© 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.)
- Published
- 2019
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14. Genetic reduction of eEF2 kinase alleviates pathophysiology in Alzheimer's disease model mice.
- Author
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Beckelman BC, Yang W, Kasica NP, Zimmermann HR, Zhou X, Keene CD, Ryazanov AG, and Ma T
- Subjects
- Animals, Disease Models, Animal, Female, Humans, Male, Mice, Mice, Knockout, Peptide Elongation Factor 2 genetics, Peptide Elongation Factor 2 metabolism, Phosphorylation genetics, Alzheimer Disease genetics, Alzheimer Disease metabolism, Alzheimer Disease pathology, Dendritic Spines genetics, Dendritic Spines metabolism, Dendritic Spines pathology, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase metabolism, Long-Term Potentiation, Post-Synaptic Density genetics, Post-Synaptic Density metabolism, Post-Synaptic Density pathology, Signal Transduction genetics
- Abstract
Molecular signaling mechanisms underlying Alzheimer's disease (AD) remain unclear. Maintenance of memory and synaptic plasticity depend on de novo protein synthesis, dysregulation of which is implicated in AD. Recent studies showed AD-associated hyperphosphorylation of mRNA translation factor eukaryotic elongation factor 2 (eEF2), which results in inhibition of protein synthesis. We tested to determine whether suppression of eEF2 phosphorylation could improve protein synthesis capacity and AD-associated cognitive and synaptic impairments. Genetic reduction of the eEF2 kinase (eEF2K) in 2 AD mouse models suppressed AD-associated eEF2 hyperphosphorylation and improved memory deficits and hippocampal long-term potentiation (LTP) impairments without altering brain amyloid β (Aβ) pathology. Furthermore, eEF2K reduction alleviated AD-associated defects in dendritic spine morphology, postsynaptic density formation, de novo protein synthesis, and dendritic polyribosome assembly. Our results link eEF2K/eEF2 signaling dysregulation to AD pathophysiology and therefore offer a feasible therapeutic target.
- Published
- 2019
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15. Genetic removal of eIF2α kinase PERK in mice enables hippocampal L-LTP independent of mTORC1 activity.
- Author
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Zimmermann HR, Yang W, Beckelman BC, Kasica NP, Zhou X, Galli LD, Ryazanov AG, and Ma T
- Subjects
- Adenine analogs & derivatives, Adenine pharmacology, Animals, Anisomycin pharmacology, Biophysics, Electric Stimulation, Enzyme Inhibitors pharmacology, Female, Gene Expression Regulation drug effects, Gene Expression Regulation genetics, Hippocampus drug effects, Immunosuppressive Agents pharmacology, In Vitro Techniques, Indoles pharmacology, Long-Term Potentiation drug effects, Male, Mice, Mice, Knockout, Phosphorylation drug effects, Phosphorylation genetics, Protein Serine-Threonine Kinases genetics, Protein Synthesis Inhibitors pharmacology, Sirolimus pharmacology, Hippocampus metabolism, Long-Term Potentiation genetics, Mechanistic Target of Rapamycin Complex 1 metabolism, Protein Serine-Threonine Kinases deficiency
- Abstract
Characterization of the molecular signaling pathways underlying protein synthesis-dependent forms of synaptic plasticity, such as late long-term potentiation (L-LTP), can provide insights not only into memory expression/maintenance under physiological conditions but also potential mechanisms associated with the pathogenesis of memory disorders. Here, we report in mice that L-LTP failure induced by the mammalian (mechanistic) target of rapamycin complex 1 (mTORC1) inhibitor rapamycin is reversed by brain-specific genetic deletion of PKR-like ER kinase, PERK (PERK KO), a kinase for eukaryotic initiation factor 2α (eIF2α). In contrast, genetic removal of general control non-derepressible-2, GCN2 (GCN2 KO), another eIF2α kinase, or treatment of hippocampal slices with the PERK inhibitor GSK2606414, does not rescue rapamycin-induced L-LTP failure, suggesting mechanisms independent of eIF2α phosphorylation. Moreover, we demonstrate that phosphorylation of eukaryotic elongation factor 2 (eEF2) is significantly decreased in PERK KO mice but unaltered in GCN2 KO mice or slices treated with the PERK inhibitor. Reduction in eEF2 phosphorylation results in increased general protein synthesis, and thus could contribute to the mTORC1-independent L-LTP in PERK KO mice. We further performed experiments on mutant mice with genetic removal of eEF2K (eEF2K KO), the only known kinase for eEF2, and found that L-LTP in eEF2K KO mice is insensitive to rapamycin. These data, for the first time, connect reduction in PERK activity with the regulation of translation elongation in enabling L-LTP independent of mTORC1. Thus, our findings indicate previously unrecognized levels of complexity in the regulation of protein synthesis-dependent synaptic plasticity. Read the Editorial Highlight for this article on page 119. Cover Image for this issue: doi: 10.1111/jnc.14185., (© 2018 International Society for Neurochemistry.)
- Published
- 2018
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16. eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.
- Author
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Heise C, Taha E, Murru L, Ponzoni L, Cattaneo A, Guarnieri FC, Montani C, Mossa A, Vezzoli E, Ippolito G, Zapata J, Barrera I, Ryazanov AG, Cook J, Poe M, Stephen MR, Kopanitsa M, Benfante R, Rusconi F, Braida D, Francolini M, Proud CG, Valtorta F, Passafaro M, Sala M, Bachi A, Verpelli C, Rosenblum K, and Sala C
- Subjects
- Animals, Cells, Cultured, Cerebral Cortex drug effects, Cerebral Cortex enzymology, Cerebral Cortex pathology, Conditioning, Psychological physiology, Disease Models, Animal, Elongation Factor 2 Kinase antagonists & inhibitors, Elongation Factor 2 Kinase genetics, Epilepsy pathology, Fear physiology, Hippocampus drug effects, Hippocampus enzymology, Hippocampus pathology, Mice, Inbred C57BL, Mice, Knockout, Neural Inhibition drug effects, Neural Inhibition physiology, Neurons drug effects, Neurons pathology, Rats, Sprague-Dawley, Receptors, GABA-A metabolism, Synapsins genetics, Synapsins metabolism, Synaptic Transmission drug effects, gamma-Aminobutyric Acid metabolism, Elongation Factor 2 Kinase metabolism, Epilepsy enzymology, Neurons enzymology, Synaptic Transmission physiology
- Abstract
Alterations in the balance of inhibitory and excitatory synaptic transmission have been implicated in the pathogenesis of neurological disorders such as epilepsy. Eukaryotic elongation factor 2 kinase (eEF2K) is a highly regulated, ubiquitous kinase involved in the control of protein translation. Here, we show that eEF2K activity negatively regulates GABAergic synaptic transmission. Indeed, loss of eEF2K increases GABAergic synaptic transmission by upregulating the presynaptic protein Synapsin 2b and α5-containing GABAA receptors and thus interferes with the excitation/inhibition balance. This cellular phenotype is accompanied by an increased resistance to epilepsy and an impairment of only a specific hippocampal-dependent fear conditioning. From a clinical perspective, our results identify eEF2K as a potential novel target for antiepileptic drugs, since pharmacological and genetic inhibition of eEF2K can revert the epileptic phenotype in a mouse model of human epilepsy., (© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2017
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17. Epithelial magnesium transport by TRPM6 is essential for prenatal development and adult survival.
- Author
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Chubanov V, Ferioli S, Wisnowsky A, Simmons DG, Leitzinger C, Einer C, Jonas W, Shymkiv Y, Bartsch H, Braun A, Akdogan B, Mittermeier L, Sytik L, Torben F, Jurinovic V, van der Vorst EP, Weber C, Yildirim ÖA, Sotlar K, Schürmann A, Zierler S, Zischka H, Ryazanov AG, and Gudermann T
- Subjects
- Animals, Female, Gene Knockout Techniques, Mice, Placenta enzymology, Placenta metabolism, Pregnancy, Survival Analysis, TRPM Cation Channels genetics, Yolk Sac enzymology, Yolk Sac metabolism, Embryonic Development, Intestinal Mucosa enzymology, Intestinal Mucosa metabolism, Magnesium metabolism, TRPM Cation Channels metabolism
- Abstract
Mg
2+ regulates many physiological processes and signalling pathways. However, little is known about the mechanisms underlying the organismal balance of Mg2+ . Capitalizing on a set of newly generated mouse models, we provide an integrated mechanistic model of the regulation of organismal Mg2+ balance during prenatal development and in adult mice by the ion channel TRPM6. We show that TRPM6 activity in the placenta and yolk sac is essential for embryonic development. In adult mice, TRPM6 is required in the intestine to maintain organismal Mg2+ balance, but is dispensable in the kidney. Trpm6 inactivation in adult mice leads to a shortened lifespan, growth deficit and metabolic alterations indicative of impaired energy balance. Dietary Mg2+ supplementation not only rescues all phenotypes displayed by Trpm6 -deficient adult mice, but also may extend the lifespan of wildtype mice. Hence, maintenance of organismal Mg2+ balance by TRPM6 is crucial for prenatal development and survival to adulthood., Competing Interests: The authors declare that no competing interests exist.- Published
- 2016
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18. Pharmacological eEF2K activation promotes cell death and inhibits cancer progression.
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De Gassart A, Demaria O, Panes R, Zaffalon L, Ryazanov AG, Gilliet M, and Martinon F
- Subjects
- AMP-Activated Protein Kinases metabolism, Animals, Cell Death drug effects, Cell Death genetics, Cell Line, Cell Survival drug effects, Cell Survival genetics, Disease Models, Animal, Disease Progression, Dose-Response Relationship, Drug, Drug Resistance genetics, Elongation Factor 2 Kinase genetics, Female, Gene Expression, Humans, Mechanistic Target of Rapamycin Complex 1, Mice, Mice, Knockout, Multiprotein Complexes metabolism, Nelfinavir chemistry, Nelfinavir pharmacology, Neoplasms genetics, Peptide Elongation Factor 2 metabolism, Phosphorylation, Protein Biosynthesis, TOR Serine-Threonine Kinases metabolism, Tumor Burden, Elongation Factor 2 Kinase metabolism, Neoplasms metabolism, Neoplasms pathology
- Abstract
Activation of the elongation factor 2 kinase (eEF2K) leads to the phosphorylation and inhibition of the elongation factor eEF2, reducing mRNA translation rates. Emerging evidence indicates that the regulation of factors involved in protein synthesis may be critical for controlling diverse biological processes including cancer progression. Here we show that inhibitors of the HIV aspartyl protease (HIV-PIs), nelfinavir in particular, trigger a robust activation of eEF2K leading to the phosphorylation of eEF2. Beyond its anti-viral effects, nelfinavir has antitumoral activity and promotes cell death. We show that nelfinavir-resistant cells specifically evade eEF2 inhibition. Decreased cell viability induced by nelfinavir is impaired in cells lacking eEF2K. Moreover, nelfinavir-mediated anti-tumoral activity is severely compromised in eEF2K-deficient engrafted tumors in vivo Our findings imply that exacerbated activation of eEF2K is detrimental for tumor survival and describe a mechanism explaining the anti-tumoral properties of HIV-PIs., (© 2016 The Authors.)
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- 2016
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19. Paradoxical Roles of Elongation Factor-2 Kinase in Stem Cell Survival.
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Liao Y, Chu HP, Hu Z, Merkin JJ, Chen J, Liu Z, Degenhardt K, White E, and Ryazanov AG
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- Animals, Apoptosis genetics, Apoptosis radiation effects, Cell Survival genetics, Cell Survival radiation effects, Mice, Mice, Knockout, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase metabolism, Gamma Rays adverse effects, Intestine, Small metabolism, Intestine, Small pathology, Mitosis genetics, Mitosis radiation effects, Radiation Injuries, Experimental genetics, Radiation Injuries, Experimental metabolism, Radiation Injuries, Experimental pathology, Radiation Injuries, Experimental prevention & control, Radiation Tolerance genetics, Radiation Tolerance radiation effects, Stem Cells metabolism, Stem Cells pathology
- Abstract
Protein synthesis inhibition is an immediate response during stress to switch the composition of protein pool in order to adapt to the new environment. It was reported that this response could be either protective or deleterious. However, how cells choose to live or die upon protein synthesis inhibition is largely unknown. Previously, we have shown that elongation factor-2 kinase (eEF2K), a protein kinase that suppresses protein synthesis during elongation phase, is a positive regulator of apoptosis both in vivo and in vitro Consistently, here we report that knock-out of eEF2K protects mice from a lethal dose of whole-body ionizing radiation at 8 Gy by reducing apoptosis levels in both bone marrow and gastrointestinal tracts. Surprisingly, similar to the loss of p53, eEF2K deficiency results in more severe damage to the gastrointestinal tract at 20 Gy with the increased mitotic cell death in small intestinal stem cells. Furthermore, using epithelial cell lines, we showed that eEF2K is required for G2/M arrest induced by radiation to prevent mitotic catastrophe in a p53-independent manner. Specifically, we observed the elevation of Akt/ERK activity as well as the reduction of p21 expression in Eef2k(-/-) cells. Therefore, eEF2K also provides a protective strategy to maintain genomic integrity by arresting cell cycle in response to stress. Our results suggest that protective versus pro-apoptotic roles of eEF2K depend on the type of cells: eEF2K is protective in highly proliferative cells, such as small intestinal stem cells and cancer cells, which are more susceptible to mitotic catastrophe., (© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.)
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- 2016
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20. A Critical Role of TRPM7 As an Ion Channel Protein in Mediating the Mineralization of the Craniofacial Hard Tissues.
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Nakano Y, Le MH, Abduweli D, Ho SP, Ryazanova LV, Hu Z, Ryazanov AG, Den Besten PK, and Zhang Y
- Abstract
Magnesium ion (Mg(2+)) is the fourth most common cation in the human body, and has a crucial role in many physiological functions. Mg(2+) homeostasis is an important contributor to bone development, however, its roles in the development of dental mineralized tissues have not yet been well known. We identified that transient receptor potential cation channel, subfamily M, member 7 (TRPM7), was significantly upregulated in the mature ameloblasts as compared to other ameloblasts through our whole transcript microarray analyses of the ameloblasts. TRPM7, an ion channel for divalent metal cations with an intrinsic serine/threonine protein kinase activity, has been characterized as a key regulator of whole body Mg(2+) homeostasis. Semi-quantitative PCR and immunostaining for TRMP7 confirmed its upregulation during the maturation stage of enamel formation, at which ameloblasts direct rapid mineralization of the enamel matrix. The significantly hypomineralized craniofacial structures, including incisors, molars, and cranial bones were demonstrated by microCT analysis, von Kossa and trichrome staining in Trpm7 (Δkinase∕+) mice. A previously generated heterozygous mouse model with the deletion of the TRPM7 kinase domain. Interestingly, the skeletal phenotype of Trpm7 (Δkinase∕+) mice resembled those found in the tissue-nonspecific alkaline phosphatase (Alpl) KO mice, thus we further examined whether ALPL protein content and alkaline phosphatase (ALPase) activity in ameloblasts, odontoblasts and osteoblasts were affected in those mice. While ALPL protein in Trpm7 (Δkinase∕+) mice remained at the similar level as that in wt mice, ALPase activities in the Trpm7 (Δkinase∕+) mice were almost nonexistent. Supplemented magnesium successfully rescued the activities of ALPase in ameloblasts, odontoblasts and osteoblasts of Trpm7 (Δkinase∕+) mice. These results suggested that TRPM7 is essential for mineralization of enamel as well as dentin and bone by providing sufficient Mg(2+) for the ALPL activity, underlining the key importance of ALPL for biomineralization.
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- 2016
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21. TRPM7 kinase activity regulates murine mast cell degranulation.
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Zierler S, Sumoza-Toledo A, Suzuki S, Dúill FÓ, Ryazanova LV, Penner R, Ryazanov AG, and Fleig A
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- Animals, Cells, Cultured, Enzyme Activation physiology, Female, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, TRPM Cation Channels genetics, Cell Degranulation physiology, Mast Cells metabolism, TRPM Cation Channels metabolism
- Abstract
Key Points: The Mg(2+) and Ca(2+) conducting transient receptor potential melastatin 7 (TRPM7) channel-enzyme (chanzyme) has been implicated in immune cell function. Mice heterozygous for a TRPM7 kinase deletion are hyperallergic, while mice with a single point mutation at amino acid 1648, silencing kinase activity, are not. As mast cell mediators trigger allergic reactions, we here determine the function of TRPM7 in mast cell degranulation and histamine release. Our data establish that TRPM7 kinase activity regulates mast cell degranulation and release of histamine independently of TRPM7 channel function. Our findings suggest a regulatory role of TRPM7 kinase activity on intracellular Ca(2+) and extracellular Mg(2+) sensitivity of mast cell degranulation., Abstract: Transient receptor potential melastatin 7 (TRPM7) is a divalent ion channel with a C-terminally located α-kinase. Mice heterozygous for a TRPM7 kinase deletion (TRPM7(+/∆K) ) are hypomagnesaemic and hyperallergic. In contrast, mice carrying a single point mutation at amino acid 1648, which silences TRPM7 kinase activity (TRPM7(KR) ), are not hyperallergic and are resistant to systemic magnesium (Mg(2+) ) deprivation. Since allergic reactions are triggered by mast cell-mediated histamine release, we investigated the function of TRPM7 on mast cell degranulation and histamine release using wild-type (TRPM7(+/+) ), TRPM7(+/∆K) and TRPM7(KR) mice. We found that degranulation and histamine release proceeded independently of TRPM7 channel function. Furthermore, extracellular Mg(2+) assured unperturbed IgE-DNP-dependent exocytosis, independently of TRPM7. However, impairment of TRPM7 kinase function suppressed IgE-DNP-dependent exocytosis, slowed the cellular degranulation rate, and diminished the sensitivity to intracellular calcium (Ca(2+) ) in G protein-induced exocytosis. In addition, G protein-coupled receptor (GPCR) stimulation revealed strong suppression of histamine release, whereas removal of extracellular Mg(2+) caused the phenotype to revert. We conclude that the TRPM7 kinase activity regulates murine mast cell degranulation by changing its sensitivity to intracellular Ca(2+) and affecting granular mobility and/or histamine contents., (© 2015 The Authors. The Journal of Physiology © 2015 The Physiological Society.)
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- 2016
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22. Transient Receptor Potential Melastatin 7 Cation Channel Kinase: New Player in Angiotensin II-Induced Hypertension.
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Antunes TT, Callera GE, He Y, Yogi A, Ryazanov AG, Ryazanova LV, Zhai A, Stewart DJ, Shrier A, and Touyz RM
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- Analysis of Variance, Animals, Cardiomegaly physiopathology, Disease Models, Animal, Disease Progression, Gene Expression Regulation, Hypertension chemically induced, Male, Mice, Mice, Inbred C57BL, Muscle, Smooth, Vascular metabolism, Random Allocation, Risk Assessment, TRPM Cation Channels metabolism, Up-Regulation, Ventricular Dysfunction, Left physiopathology, Angiotensin II pharmacology, Cardiomegaly metabolism, Hypertension physiopathology, Reactive Oxygen Species metabolism, TRPM Cation Channels genetics, Ventricular Dysfunction, Left metabolism
- Abstract
Transient receptor potential melastatin 7 (TRPM7) is a bifunctional protein comprising a magnesium (Mg(2+))/cation channel and a kinase domain. We previously demonstrated that vasoactive agents regulate vascular TRPM7. Whether TRPM7 plays a role in the pathophysiology of hypertension and associated cardiovascular dysfunction is unknown. We studied TRPM7 kinase-deficient mice (TRPM7Δkinase; heterozygous for TRPM7 kinase) and wild-type (WT) mice infused with angiotensin II (Ang II; 400 ng/kg per minute, 4 weeks). TRPM7 kinase expression was lower in heart and aorta from TRPM7Δkinase versus WT mice, effects that were further reduced by Ang II infusion. Plasma Mg(2+) was lower in TRPM7Δkinase versus WT mice in basal and stimulated conditions. Ang II increased blood pressure in both strains with exaggerated responses in TRPM7Δkinase versus WT groups (P<0.05). Acetylcholine-induced vasorelaxation was reduced in Ang II-infused TRPM7Δkinase mice, an effect associated with Akt and endothelial nitric oxide synthase downregulation. Vascular cell adhesion molecule-1 expression was increased in Ang II-infused TRPM7 kinase-deficient mice. TRPM7 kinase targets, calpain, and annexin-1, were activated by Ang II in WT but not in TRPM7Δkinase mice. Echocardiographic and histopathologic analysis demonstrated cardiac hypertrophy and left ventricular dysfunction in Ang II-treated groups. In TRPM7 kinase-deficient mice, Ang II-induced cardiac functional and structural effects were amplified compared with WT counterparts. Our data demonstrate that in TRPM7Δkinase mice, Ang II-induced hypertension is exaggerated, cardiac remodeling and left ventricular dysfunction are amplified, and endothelial function is impaired. These processes are associated with hypomagnesemia, blunted TRPM7 kinase expression/signaling, endothelial nitric oxide synthase downregulation, and proinflammatory vascular responses. Our findings identify TRPM7 kinase as a novel player in Ang II-induced hypertension and associated vascular and target organ damage., (© 2016 American Heart Association, Inc.)
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- 2016
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23. mTORC1-mediated translational elongation limits intestinal tumour initiation and growth.
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Faller WJ, Jackson TJ, Knight JR, Ridgway RA, Jamieson T, Karim SA, Jones C, Radulescu S, Huels DJ, Myant KB, Dudek KM, Casey HA, Scopelliti A, Cordero JB, Vidal M, Pende M, Ryazanov AG, Sonenberg N, Meyuhas O, Hall MN, Bushell M, Willis AE, and Sansom OJ
- Subjects
- Adenomatous Polyposis Coli Protein deficiency, Adenomatous Polyposis Coli Protein genetics, Animals, Cell Proliferation, Cell Transformation, Neoplastic metabolism, Elongation Factor 2 Kinase deficiency, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase metabolism, Enzyme Activation, Genes, APC, Intestinal Neoplasms genetics, Male, Mechanistic Target of Rapamycin Complex 1, Mice, Mice, Inbred C57BL, Oncogene Protein p55(v-myc) metabolism, Peptide Elongation Factor 2 metabolism, Ribosomal Protein S6 Kinases metabolism, Signal Transduction, Wnt Proteins metabolism, Cell Transformation, Neoplastic pathology, Intestinal Neoplasms metabolism, Intestinal Neoplasms pathology, Multiprotein Complexes metabolism, Peptide Chain Elongation, Translational, TOR Serine-Threonine Kinases metabolism
- Abstract
Inactivation of APC is a strongly predisposing event in the development of colorectal cancer, prompting the search for vulnerabilities specific to cells that have lost APC function. Signalling through the mTOR pathway is known to be required for epithelial cell proliferation and tumour growth, and the current paradigm suggests that a critical function of mTOR activity is to upregulate translational initiation through phosphorylation of 4EBP1 (refs 6, 7). This model predicts that the mTOR inhibitor rapamycin, which does not efficiently inhibit 4EBP1 (ref. 8), would be ineffective in limiting cancer progression in APC-deficient lesions. Here we show in mice that mTOR complex 1 (mTORC1) activity is absolutely required for the proliferation of Apc-deficient (but not wild-type) enterocytes, revealing an unexpected opportunity for therapeutic intervention. Although APC-deficient cells show the expected increases in protein synthesis, our study reveals that it is translation elongation, and not initiation, which is the rate-limiting component. Mechanistically, mTORC1-mediated inhibition of eEF2 kinase is required for the proliferation of APC-deficient cells. Importantly, treatment of established APC-deficient adenomas with rapamycin (which can target eEF2 through the mTORC1-S6K-eEF2K axis) causes tumour cells to undergo growth arrest and differentiation. Taken together, our data suggest that inhibition of translation elongation using existing, clinically approved drugs, such as the rapalogs, would provide clear therapeutic benefit for patients at high risk of developing colorectal cancer.
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- 2015
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24. Elucidating the role of the TRPM7 alpha-kinase: TRPM7 kinase inactivation leads to magnesium deprivation resistance phenotype in mice.
- Author
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Ryazanova LV, Hu Z, Suzuki S, Chubanov V, Fleig A, and Ryazanov AG
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- Animals, Embryo, Mammalian enzymology, Embryo, Mammalian pathology, Fibroblasts pathology, Magnesium metabolism, Magnesium Deficiency genetics, Magnesium Deficiency pathology, Mice, Mice, Mutant Strains, Protein Kinases genetics, Protein Structure, Tertiary, TRPM Cation Channels genetics, Fibroblasts enzymology, Magnesium Deficiency enzymology, Oxidative Stress, Protein Kinases metabolism, TRPM Cation Channels metabolism
- Abstract
TRPM7 is an unusual bi-functional protein containing an ion channel covalently linked to a protein kinase domain. TRPM7 is implicated in regulating cellular and systemic magnesium homeostasis. While the biophysical properties of TRPM7 ion channel and its function are relatively well characterized, the function of the TRPM7 enzymatically active kinase domain is not understood yet. To investigate the physiological role of TRPM7 kinase activity, we constructed mice carrying an inactive TRPM7 kinase. We found that these mice were resistant to dietary magnesium deprivation, surviving three times longer than wild type mice; also they displayed decreased chemically induced allergic reaction. Interestingly, mutant mice have lower magnesium bone content compared to wild type mice when fed regular diet; unlike wild type mice, mutant mice placed on magnesium-depleted diet did not alter their bone magnesium content. Furthermore, mouse embryonic fibroblasts isolated from TRPM7 kinase-dead animals exhibited increased resistance to magnesium deprivation and oxidative stress. Finally, electrophysiological data revealed that the activity of the kinase-dead TRPM7 channel was not significantly altered. Together, our results suggest that TRPM7 kinase is a sensor of magnesium status and provides coordination of cellular and systemic responses to magnesium deprivation.
- Published
- 2014
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25. Identification of a Mg2+-sensitive ORF in the 5'-leader of TRPM7 magnesium channel mRNA.
- Author
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Nikonorova IA, Kornakov NV, Dmitriev SE, Vassilenko KS, and Ryazanov AG
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- Base Sequence, Conserved Sequence, HEK293 Cells, Humans, 5' Untranslated Regions, Gene Expression Regulation, Magnesium pharmacology, Open Reading Frames, Protein Biosynthesis drug effects, TRPM Cation Channels genetics
- Abstract
TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg2+. Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that act together to drastically inhibit translation of the TRPM7 reading frame at high magnesium levels and ensure its optimal translation at low magnesium levels, when the activity of the channel-kinase is most required. The study provides the first example of magnesium channel synthesis being controlled by Mg2+ in higher eukaryotes., (© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2014
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26. Germline quality control: eEF2K stands guard to eliminate defective oocytes.
- Author
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Chu HP, Liao Y, Novak JS, Hu Z, Merkin JJ, Shymkiv Y, Braeckman BP, Dorovkov MV, Nguyen A, Clifford PM, Nagele RG, Harrison DE, Ellis RE, and Ryazanov AG
- Subjects
- Animals, Blotting, Western, Caenorhabditis elegans cytology, Caspases metabolism, Cells, Cultured, Embryo, Mammalian cytology, Embryo, Mammalian metabolism, Female, Fibroblasts cytology, Fibroblasts metabolism, Immunoenzyme Techniques, In Situ Nick-End Labeling, Male, Mice, Mice, Knockout, NIH 3T3 Cells, Oocytes cytology, Ovary cytology, Ovary physiology, Phosphorylation, Apoptosis, Caenorhabditis elegans physiology, Elongation Factor 2 Kinase physiology, Germ Cells pathology, Oocytes physiology, Quality Control
- Abstract
The control of germline quality is critical to reproductive success and survival of a species; however, the mechanisms underlying this process remain unknown. Here, we demonstrate that elongation factor 2 kinase (eEF2K), an evolutionarily conserved regulator of protein synthesis, functions to maintain germline quality and eliminate defective oocytes. We show that disruption of eEF2K in mice reduces ovarian apoptosis and results in the accumulation of aberrant follicles and defective oocytes at advanced reproductive age. Furthermore, the loss of eEF2K in Caenorhabditis elegans results in a reduction of germ cell death and significant decline in oocyte quality and embryonic viability. Examination of the mechanisms by which eEF2K regulates apoptosis shows that eEF2K senses oxidative stress and quickly downregulates short-lived antiapoptotic proteins, XIAP and c-FLIPL by inhibiting global protein synthesis. These results suggest that eEF2K-mediated inhibition of protein synthesis renders cells susceptible to apoptosis and functions to eliminate suboptimal germ cells., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
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27. Acute suppression of spontaneous neurotransmission drives synaptic potentiation.
- Author
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Nosyreva E, Szabla K, Autry AE, Ryazanov AG, Monteggia LM, and Kavalali ET
- Subjects
- Analysis of Variance, Animals, Animals, Newborn, Biophysics, Brain-Derived Neurotrophic Factor deficiency, Electric Stimulation, Elongation Factor 2 Kinase deficiency, Enzyme Inhibitors pharmacology, Evoked Potentials genetics, Evoked Potentials physiology, Excitatory Amino Acid Agonists pharmacology, Excitatory Amino Acid Antagonists pharmacology, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials genetics, Exploratory Behavior drug effects, Exploratory Behavior physiology, Feeding Behavior drug effects, Feeding Behavior physiology, GABA Antagonists pharmacology, Gene Expression Regulation drug effects, Gene Expression Regulation genetics, Glutamic Acid metabolism, Hippocampus physiology, In Vitro Techniques, Ketamine pharmacology, Locomotion drug effects, Locomotion genetics, Mice, Mice, Knockout, Neural Inhibition drug effects, Patch-Clamp Techniques, Picrotoxin pharmacology, Rats, Rats, Sprague-Dawley, Receptors, AMPA metabolism, Sodium Channel Blockers pharmacology, Swimming physiology, Tetrodotoxin pharmacology, Time Factors, Hippocampus cytology, Inhibition, Psychological, Neural Inhibition physiology, Neuronal Plasticity physiology, Synapses physiology, Synaptic Transmission physiology
- Abstract
The impact of spontaneous neurotransmission on neuronal plasticity remains poorly understood. Here, we show that acute suppression of spontaneous NMDA receptor-mediated (NMDAR-mediated) neurotransmission potentiates synaptic responses in the CA1 regions of rat and mouse hippocampus. This potentiation requires protein synthesis, brain-derived neurotrophic factor expression, eukaryotic elongation factor-2 kinase function, and increased surface expression of AMPA receptors. Our behavioral studies link this same synaptic signaling pathway to the fast-acting antidepressant responses elicited by ketamine. We also show that selective neurotransmitter depletion from spontaneously recycling vesicles triggers synaptic potentiation via the same pathway as NMDAR blockade, demonstrating that presynaptic impairment of spontaneous release, without manipulation of evoked neurotransmission, is sufficient to elicit postsynaptic plasticity. These findings uncover an unexpectedly dynamic impact of spontaneous glutamate release on synaptic efficacy and provide new insight into a key synaptic substrate for rapid antidepressant action.
- Published
- 2013
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28. Phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase.
- Author
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Hizli AA, Chi Y, Swanger J, Carter JH, Liao Y, Welcker M, Ryazanov AG, and Clurman BE
- Subjects
- Amino Acid Sequence, Cell Line, Humans, Mitosis, Molecular Sequence Data, Peptide Elongation Factor 2 chemistry, Peptide Elongation Factor 2 genetics, Phosphorylation, Point Mutation, Serine chemistry, Serine genetics, Threonine chemistry, Threonine metabolism, Cyclin A metabolism, Cyclin-Dependent Kinase 2 metabolism, Elongation Factor 2 Kinase metabolism, Peptide Elongation Factor 2 metabolism, Serine metabolism
- Abstract
Protein synthesis is highly regulated via both initiation and elongation. One mechanism that inhibits elongation is phosphorylation of eukaryotic elongation factor 2 (eEF2) on threonine 56 (T56) by eEF2 kinase (eEF2K). T56 phosphorylation inactivates eEF2 and is the only known normal eEF2 functional modification. In contrast, eEF2K undergoes extensive regulatory phosphorylations that allow diverse pathways to impact elongation. We describe a new mode of eEF2 regulation and show that its phosphorylation by cyclin A-cyclin-dependent kinase 2 (CDK2) on a novel site, serine 595 (S595), directly regulates T56 phosphorylation by eEF2K. S595 phosphorylation varies during the cell cycle and is required for efficient T56 phosphorylation in vivo. Importantly, S595 phosphorylation by cyclin A-CDK2 directly stimulates eEF2 T56 phosphorylation by eEF2K in vitro, and we suggest that S595 phosphorylation facilitates T56 phosphorylation by recruiting eEF2K to eEF2. S595 phosphorylation is thus the first known eEF2 modification that regulates its inhibition by eEF2K and provides a novel mechanism linking the cell cycle machinery to translational control. Because all known eEF2 regulation is exerted via eEF2K, S595 phosphorylation may globally couple the cell cycle machinery to regulatory pathways that impact eEF2K activity.
- Published
- 2013
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29. Construction of human activity-based phosphorylation networks.
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Newman RH, Hu J, Rho HS, Xie Z, Woodard C, Neiswinger J, Cooper C, Shirley M, Clark HM, Hu S, Hwang W, Jeong JS, Wu G, Lin J, Gao X, Ni Q, Goel R, Xia S, Ji H, Dalby KN, Birnbaum MJ, Cole PA, Knapp S, Ryazanov AG, Zack DJ, Blackshaw S, Pawson T, Gingras AC, Desiderio S, Pandey A, Turk BE, Zhang J, Zhu H, and Qian J
- Subjects
- Agammaglobulinaemia Tyrosine Kinase, Algorithms, Amino Acid Sequence, B-Lymphocytes cytology, Bayes Theorem, Cyclic AMP-Dependent Protein Kinases genetics, Humans, Molecular Sequence Data, Phosphorylation, Protein Array Analysis, Protein Interaction Maps, Protein-Tyrosine Kinases genetics, Receptors, Antigen, B-Cell genetics, Tyrosine metabolism, B-Lymphocytes enzymology, Cyclic AMP-Dependent Protein Kinases metabolism, Protein-Tyrosine Kinases metabolism, Receptors, Antigen, B-Cell metabolism, Signal Transduction genetics
- Abstract
The landscape of human phosphorylation networks has not been systematically explored, representing vast, unchartered territories within cellular signaling networks. Although a large number of in vivo phosphorylated residues have been identified by mass spectrometry (MS)-based approaches, assigning the upstream kinases to these residues requires biochemical analysis of kinase-substrate relationships (KSRs). Here, we developed a new strategy, called CEASAR, based on functional protein microarrays and bioinformatics to experimentally identify substrates for 289 unique kinases, resulting in 3656 high-quality KSRs. We then generated consensus phosphorylation motifs for each of the kinases and integrated this information, along with information about in vivo phosphorylation sites determined by MS, to construct a high-resolution map of phosphorylation networks that connects 230 kinases to 2591 in vivo phosphorylation sites in 652 substrates. The value of this data set is demonstrated through the discovery of a new role for PKA downstream of Btk (Bruton's tyrosine kinase) during B-cell receptor signaling. Overall, these studies provide global insights into kinase-mediated signaling pathways and promise to advance our understanding of cellular signaling processes in humans.
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- 2013
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30. Purification and characterization of tagless recombinant human elongation factor 2 kinase (eEF-2K) expressed in Escherichia coli.
- Author
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Abramczyk O, Tavares CD, Devkota AK, Ryazanov AG, Turk BE, Riggs AF, Ozpolat B, and Dalby KN
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- Amino Acid Sequence, Calcium metabolism, Calmodulin genetics, Calmodulin metabolism, Chromatography, Affinity, Chromatography, Gel, Chromatography, Ion Exchange, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase isolation & purification, Endopeptidases metabolism, Escherichia coli, Histidine metabolism, Humans, Kinetics, Molecular Sequence Data, Oligopeptides metabolism, Phosphorylation, Plasmids chemistry, Plasmids metabolism, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Thioredoxins metabolism, Transformation, Bacterial, Cloning, Molecular methods, Elongation Factor 2 Kinase metabolism, Peptide Elongation Factor 2 metabolism, Plasmids genetics, Protein Biosynthesis genetics, Recombinant Proteins metabolism
- Abstract
The eukaryotic elongation factor 2 kinase (eEF-2K) modulates the rate of protein synthesis by impeding the elongation phase of translation by inactivating the eukaryotic elongation factor 2 (eEF-2) via phosphorylation. eEF-2K is known to be activated by calcium and calmodulin, whereas the mTOR and MAPK pathways are suggested to negatively regulate kinase activity. Despite its pivotal role in translation regulation and potential role in tumor survival, the structure, function, and regulation of eEF-2K have not been described in detail. This deficiency may result from the difficulty of obtaining the recombinant kinase in a form suitable for biochemical analysis. Here we report the purification and characterization of recombinant human eEF-2K expressed in the Escherichia coli strain Rosetta-gami 2(DE3). Successive chromatography steps utilizing Ni-NTA affinity, anion-exchange, and gel filtration columns accomplished purification. Cleavage of the thioredoxin-His(6)-tag from the N-terminus of the expressed kinase with TEV protease yielded 9 mg of recombinant (G-D-I)-eEF-2K per liter of culture. Light scattering shows that eEF-2K is a monomer of ∼85 kDa. In vitro kinetic analysis confirmed that recombinant human eEF-2K is able to phosphorylate wheat germ eEF-2 with kinetic parameters comparable to the mammalian enzyme., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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31. Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae.
- Author
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Bewley MA, Pham TK, Marriott HM, Noirel J, Chu HP, Ow SY, Ryazanov AG, Read RC, Whyte MK, Chain B, Wright PC, and Dockrell DH
- Subjects
- Animals, Apoptosis Regulatory Proteins metabolism, Cathepsin D genetics, Cathepsin D metabolism, Cell Cycle Proteins metabolism, Cell Line, Colony Count, Microbial, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase metabolism, Endoplasmic Reticulum physiology, Endoplasmic Reticulum Chaperone BiP, Enzyme Assays, Female, Gelsolin genetics, Gelsolin metabolism, Gene Expression Regulation, Heat-Shock Proteins metabolism, Humans, Lung microbiology, Macrophages immunology, Macrophages microbiology, Membrane Potential, Mitochondrial, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondrial Membrane Transport Proteins metabolism, Oxidative Stress, Pepstatins pharmacology, Protease Inhibitors pharmacology, Reactive Oxygen Species metabolism, S100 Calcium Binding Protein A6, S100 Proteins metabolism, Streptococcus pneumoniae immunology, Superoxide Dismutase genetics, Superoxide Dismutase metabolism, Cathepsin D antagonists & inhibitors, Macrophages physiology, Proteome metabolism, Streptococcus pneumoniae physiology
- Abstract
Macrophages are central effectors of innate immune responses to bacteria. We have investigated how activation of the abundant macrophage lysosomal protease, cathepsin D, regulates the macrophage proteome during killing of Streptococcus pneumoniae. Using the cathepsin D inhibitor pepstatin A, we demonstrate that cathepsin D differentially regulates multiple targets out of 679 proteins identified and quantified by eight-plex isobaric tag for relative and absolute quantitation. Our statistical analysis identified 18 differentially expressed proteins that passed all paired t-tests (α = 0.05). This dataset was enriched for proteins regulating the mitochondrial pathway of apoptosis or inhibiting competing death programs. Five proteins were selected for further analysis. Western blotting, followed by pharmacological inhibition or genetic manipulation of cathepsin D, verified cathepsin D-dependent regulation of these proteins, after exposure to S. pneumoniae. Superoxide dismutase-2 up-regulation was temporally related to increased reactive oxygen species generation. Gelsolin, a known regulator of mitochondrial outer membrane permeabilization, was down-regulated in association with cytochrome c release from mitochondria. Eukaryotic elongation factor (eEF2), a regulator of protein translation, was also down-regulated by cathepsin D. Using absence of the negative regulator of eEF2, eEF2 kinase, we confirm that eEF2 function is required to maintain expression of the anti-apoptotic protein Mcl-1, delaying macrophage apoptosis and confirm using a murine model that maintaining eEF2 function is associated with impaired macrophage apoptosis-associated killing of Streptococcus pneumoniae. These findings demonstrate that cathepsin D regulates multiple proteins controlling the mitochondrial pathway of macrophage apoptosis or competing death processes, facilitating intracellular bacterial killing.
- Published
- 2011
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32. Phosphorylation of annexin A1 by TRPM7 kinase: a switch regulating the induction of an α-helix.
- Author
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Dorovkov MV, Kostyukova AS, and Ryazanov AG
- Subjects
- Amino Acid Sequence, Animals, Cell Membrane metabolism, Molecular Sequence Data, Peptide Fragments chemistry, Peptide Fragments metabolism, Phosphorylation, Protein Structure, Secondary, S100 Proteins metabolism, Serine, Annexin A1 chemistry, Annexin A1 metabolism, TRPM Cation Channels metabolism
- Abstract
TRPM7 is an unusual bifunctional protein consisting of an α-kinase domain fused to a TRP ion channel. Previously, we have identified annexin A1 as a substrate for TRPM7 kinase and found that TRPM7 phosphorylates annexin A1 at Ser5 within the N-terminal α-helix. Annexin A1 is a Ca(2+)-dependent membrane binding protein, which has been implicated in membrane trafficking and reorganization. The N-terminal tail of annexin A1 can interact with either membranes or S100A11 protein, and it adopts the conformation of an amphipathic α-helix upon these interactions. Moreover, the existing evidence indicates that the formation of an α-helix is essential for these interactions. Here we show that phosphorylation at Ser5 prevents the N-terminal peptide of annexin A1 from adopting an α-helical conformation in the presence of membrane-mimetic micelles as well as phospholipid vesicles. We also show that phosphorylation at Ser5 dramatically weakens the binding of the peptide to S100A11. Our data suggest that phosphorylation at Ser5 regulates the interaction of annexin A1 with membranes as well as S100A11 protein.
- Published
- 2011
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33. The channel-kinase TRPM7 regulates phosphorylation of the translational factor eEF2 via eEF2-k.
- Author
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Perraud AL, Zhao X, Ryazanov AG, and Schmitz C
- Subjects
- Animals, B-Lymphocytes enzymology, Cell Line, Chickens, Humans, Magnesium metabolism, Mice, Peptide Chain Elongation, Translational, Phosphorylation, Protein Biosynthesis, Protein Serine-Threonine Kinases, Recombinant Proteins biosynthesis, TRPM Cation Channels biosynthesis, Elongation Factor 2 Kinase metabolism, Peptide Elongation Factor 2 metabolism, TRPM Cation Channels physiology
- Abstract
Protein translation is an essential but energetically expensive process, which is carefully regulated in accordance to the cellular nutritional and energy status. Eukaryotic elongation factor 2 (eEF2) is a central regulation point since it mediates ribosomal translocation and can be inhibited by phosphorylation at Thr56. TRPM7 is the unique fusion of an ion channel with a functional Ser/Thr-kinase. While TRPM7's channel function has been implicated in regulating vertebrate Mg(2+) uptake required for cell growth, the function of its kinase domain remains unclear. Here, we show that under conditions where cell growth is limited by Mg(2+) availability, TRPM7 via its kinase mediates enhanced Thr56 phosphorylation of eEF2. TRPM7-kinase does not appear to directly phosphorylate eEF2, but rather to influence the amount of eEF2's cognate kinase eEF2-k, involving its phosphorylation at Ser77. These findings suggest that TRPM7's structural duality ensures ideal positioning of its kinase in close proximity to channel-mediated Mg(2+) uptake, allowing for the adjustment of protein translational rates to the availability of Mg(2+)., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
34. TRPM7 is essential for Mg(2+) homeostasis in mammals.
- Author
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Ryazanova LV, Rondon LJ, Zierler S, Hu Z, Galli J, Yamaguchi TP, Mazur A, Fleig A, and Ryazanov AG
- Abstract
Mg(2+) is the second-most abundant cation in animal cells and is an essential cofactor in numerous enzymatic reactions. The molecular mechanisms controlling Mg(2+) balance in the organism are not well understood. In this study, we report identification of TRPM7, a bifunctional protein containing a protein kinase fused to an ion channel, as a key regulator of whole body Mg(2+) homeostasis in mammals. We generated TRPM7-deficient mice with the deletion of the kinase domain. Homozygous TRPM7(Δkinase) mice demonstrated early embryonic lethality, whereas heterozygous mice were viable, but developed signs of hypomagnesaemia and revealed a defect in intestinal Mg(2+) absorption. Cells derived from heterozygous TRPM7(Δkinase) mice demonstrated reduced TRPM7 currents that had increased sensitivity to the inhibition by Mg(2+). Embryonic stem cells lacking TRPM7 kinase domain displayed a proliferation arrest phenotype that can be rescued by Mg(2+) supplementation. Our results demonstrate that TRPM7 is essential for the control of cellular and whole body Mg(2+) homeostasis.
- Published
- 2010
- Full Text
- View/download PDF
35. Resveratrol regulates pathologic angiogenesis by a eukaryotic elongation factor-2 kinase-regulated pathway.
- Author
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Khan AA, Dace DS, Ryazanov AG, Kelly J, and Apte RS
- Subjects
- Adenylate Kinase metabolism, Animals, Cell Movement drug effects, Cell Proliferation drug effects, Cells, Cultured, Elongation Factor 2 Kinase genetics, Endothelial Cells cytology, Endothelial Cells physiology, Endothelial Cells radiation effects, Eye blood supply, Eye metabolism, Eye pathology, Lasers, Mice, Mice, Inbred C57BL, Mice, Knockout, Resveratrol, Sirtuin 1 metabolism, Antineoplastic Agents, Phytogenic pharmacology, Elongation Factor 2 Kinase metabolism, Endothelial Cells drug effects, Neovascularization, Pathologic, Signal Transduction drug effects, Stilbenes pharmacology
- Abstract
Abnormal angiogenesis is central to the pathophysiology of diverse disease processes including cancers, ischemic and atherosclerotic heart disease, and visually debilitating eye disease. Resveratrol is a naturally occurring phytoalexin that has been demonstrated to ameliorate and decelerate the aging process as well as blunt end organ damage from obesity. These effects of resveratrol are largely mediated by members of the sirtuin family of proteins. We demonstrate that resveratrol can inhibit pathological angiogenesis in vivo and in vitro by a sirtuin-independent pathway. Resveratrol inhibits the proliferation and migration of vascular endothelial cells by activating eukaryotic elongation factor-2 kinase. The active kinase in turn phosphorylates and inactivates elongation factor-2, a key mediator of ribosomal transfer and protein translation. Functional inhibition of the kinase by gene deletion in vivo or RNA as well as pharmacological inhibition in vitro is able to completely reverse the effects of resveratrol on blood vessel growth. These studies have identified a novel and critical pathway that promotes aberrant vascular proliferation and one that is amenable to modulation by pharmacological means. In addition, these results have uncovered a sirtuin-independent pathway by which resveratrol regulates angiogenesis.
- Published
- 2010
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- View/download PDF
36. The alpha-kinases TRPM6 and TRPM7, but not eEF-2 kinase, phosphorylate the assembly domain of myosin IIA, IIB and IIC.
- Author
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Clark K, Middelbeek J, Dorovkov MV, Figdor CG, Ryazanov AG, Lasonder E, and van Leeuwen FN
- Subjects
- Amino Acid Sequence, Cell Line, Elongation Factor 2 Kinase genetics, Elongation Factor 2 Kinase metabolism, Humans, Molecular Sequence Data, Phosphorylation, Protein Serine-Threonine Kinases, Protein Structure, Tertiary, TRPM Cation Channels genetics, Myosin Heavy Chains metabolism, Myosin Type II metabolism, Nonmuscle Myosin Type IIA metabolism, Nonmuscle Myosin Type IIB metabolism, TRPM Cation Channels metabolism
- Abstract
TRPM6 and TRPM7 encode channel-kinases. While these channels share electrophysiological properties and cellular functions, TRPM6 and TRPM7 are non-redundant genes raising the possibility that the kinases have distinct substrates. Here, we demonstrate that TRPM6 and TRPM7 phosphorylate the assembly domain of myosin IIA, IIB and IIC on identical residues. Whereas phosphorylation of myosin IIA is restricted to the coiled-coil domain, TRPM6 and TRPM7 also phosphorylate the non-helical tails of myosin IIB and IIC. TRPM7 does not phosphorylate eukaryotic elongation factor-2 (eEF-2) and myosin II is a poor substrate for eEF-2 kinase. In conclusion, TRPM6 and TRPM7 share exogenous substrates among themselves but not with functionally distant alpha-kinases.
- Published
- 2008
- Full Text
- View/download PDF
37. Elongation factor 2 and fragile X mental retardation protein control the dynamic translation of Arc/Arg3.1 essential for mGluR-LTD.
- Author
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Park S, Park JM, Kim S, Kim JA, Shepherd JD, Smith-Hicks CL, Chowdhury S, Kaufmann W, Kuhl D, Ryazanov AG, Huganir RL, Linden DJ, and Worley PF
- Subjects
- Animals, Animals, Newborn, Cells, Cultured, Cycloheximide pharmacology, Dose-Response Relationship, Radiation, Electric Stimulation methods, Excitatory Amino Acid Agents pharmacology, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials physiology, Excitatory Postsynaptic Potentials radiation effects, Fragile X Mental Retardation Protein genetics, Gene Expression Regulation drug effects, Gene Expression Regulation radiation effects, Hippocampus cytology, In Vitro Techniques, Male, Mice, Mice, Knockout, Models, Biological, Neurons physiology, Patch-Clamp Techniques, Protein Biosynthesis drug effects, Protein Biosynthesis radiation effects, Protein Synthesis Inhibitors pharmacology, Protein Transport drug effects, Protein Transport physiology, Cytoskeletal Proteins metabolism, Fragile X Mental Retardation Protein physiology, Long-Term Potentiation physiology, Nerve Tissue Proteins metabolism, Peptide Elongation Factor 2 physiology, Protein Biosynthesis physiology, Receptors, AMPA physiology
- Abstract
Group I metabotropic glutamate receptors (mGluR) induce long-term depression (LTD) that requires protein synthesis. Here, we demonstrate that Arc/Arg3.1 is translationally induced within 5 min of mGluR activation, and this response is essential for mGluR-dependent LTD. The increase in Arc/Arg3.1 translation requires eEF2K, a Ca(2+)/calmodulin-dependent kinase that binds mGluR and dissociates upon mGluR activation, whereupon it phosphorylates eEF2. Phospho-eEF2 acts to slow the elongation step of translation and inhibits general protein synthesis but simultaneously increases Arc/Arg3.1 translation. Genetic deletion of eEF2K results in a selective deficit of rapid mGluR-dependent Arc/Arg3.1 translation and mGluR-LTD. This rapid translational mechanism is disrupted in the fragile X disease mouse (Fmr1 KO) in which mGluR-LTD does not require de novo protein synthesis but does require Arc/Arg3.1. We propose a model in which eEF2K-eEF2 and FMRP coordinately control the dynamic translation of Arc/Arg3.1 mRNA in dendrites that is critical for synapse-specific LTD.
- Published
- 2008
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38. TRPM7 regulates myosin IIA filament stability and protein localization by heavy chain phosphorylation.
- Author
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Clark K, Middelbeek J, Lasonder E, Dulyaninova NG, Morrice NA, Ryazanov AG, Bresnick AR, Figdor CG, and van Leeuwen FN
- Subjects
- Amino Acid Sequence, Animals, Cell Line, Chlorocebus aethiops, Conserved Sequence, Humans, Kinetics, Mice, Molecular Sequence Data, Mutation genetics, Nonmuscle Myosin Type IIA chemistry, Nonmuscle Myosin Type IIA genetics, Phosphorylation, Phosphoserine metabolism, Phosphothreonine metabolism, Sequence Alignment, TRPM Cation Channels genetics, Myosin Heavy Chains metabolism, Nonmuscle Myosin Type IIA metabolism, TRPM Cation Channels metabolism
- Abstract
Deregulation of myosin II-based contractility contributes to the pathogenesis of human diseases, such as cancer, which underscores the necessity for tight spatial and temporal control of myosin II activity. Recently, we demonstrated that activation of the mammalian alpha-kinase TRPM7 inhibits myosin II-based contractility in a Ca(2+)- and kinase-dependent manner. However, the molecular mechanism is poorly defined. Here, we demonstrate that TRPM7 phosphorylates the COOH-termini of both mouse and human myosin IIA heavy chains--the COOH-terminus being a region that is critical for filament stability. Phosphorylated residues were mapped to Thr1800, Ser1803 and Ser1808. Mutation of these residues to alanine and that to aspartic acid lead to an increase and a decrease, respectively, in myosin IIA incorporation into the actomyosin cytoskeleton and accordingly affect subcellular localization. In conclusion, our data demonstrate that TRPM7 regulates myosin IIA filament stability and localization by phosphorylating a short stretch of amino acids within the alpha-helical tail of the myosin IIA heavy chain.
- Published
- 2008
- Full Text
- View/download PDF
39. A pharmacoproteomic approach implicates eukaryotic elongation factor 2 kinase in ER stress-induced cell death.
- Author
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Boyce M, Py BF, Ryazanov AG, Minden JS, Long K, Ma D, and Yuan J
- Subjects
- Animals, Cells, Cultured, Eukaryotic Initiation Factor-2 metabolism, Mice, PC12 Cells, Proteomics, Rats, Signal Transduction, Thiourea pharmacology, Apoptosis, Cinnamates pharmacology, Elongation Factor 2 Kinase metabolism, Endoplasmic Reticulum metabolism, Peptide Elongation Factor 2 metabolism, Thiourea analogs & derivatives
- Abstract
Apoptosis triggered by endoplasmic reticulum (ER) stress has been implicated in many diseases but its cellular regulation remains poorly understood. Previously, we identified salubrinal (sal), a small molecule that protects cells from ER stress-induced apoptosis by selectively activating a subset of endogenous ER stress-signaling events. Here, we use sal as a probe in a proteomic approach to discover new information about the endogenous cellular response to ER stress. We show that sal induces phosphorylation of the translation elongation factor eukaryotic translation elongation factor 2 (eEF-2), an event that depends on eEF-2 kinase (eEF-2K). ER stress itself also induces eEF-2K-dependent eEF-2 phosphorylation, and this pathway promotes translational arrest and cell death in this context, identifying eEF-2K as a hitherto unknown regulator of ER stress-induced apoptosis. Finally, we use both sal and ER stress models to show that eEF-2 phosphorylation can be activated by at least two signaling mechanisms. Our work identifies eEF-2K as a new component of the ER stress response and underlines the utility of novel small molecules in discovering new cell biology.
- Published
- 2008
- Full Text
- View/download PDF
40. Autophagy regulates ageing in C. elegans.
- Author
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Hars ES, Qi H, Ryazanov AG, Jin S, Cai L, Hu C, and Liu LF
- Subjects
- Animals, Caenorhabditis elegans Proteins genetics, Caenorhabditis elegans Proteins metabolism, Proportional Hazards Models, RNA Interference, Survival Analysis, Aging physiology, Autophagy physiology, Caenorhabditis elegans physiology, Longevity physiology
- Abstract
The role of autophagy in ageing regulation has been suggested based on studies in C. elegans, in which knockdown of the expression of bec-1 (ortholog of the yeast and mammalian autophagy genes ATG6/VPS30 and beclin 1, respectively) shortens lifespan of the daf-2(e1370) mutant C. elegans. However, Beclin1/ATG6 is also known to be involved in other cellular functions in addition to autophagy. In the current study, we knocked down two other autophagy genes, atg-7 and atg-12, in C. elegans using RNAi. We showed that RNAi shortened the lifespan of both wild type and daf-2 mutant C. elegans, providing strong support for a role of autophagy in ageing regulation.
- Published
- 2007
- Full Text
- View/download PDF
41. TRPM7, a novel regulator of actomyosin contractility and cell adhesion.
- Author
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Clark K, Langeslag M, van Leeuwen B, Ran L, Ryazanov AG, Figdor CG, Moolenaar WH, Jalink K, and van Leeuwen FN
- Subjects
- Actomyosin metabolism, Animals, Calcium metabolism, Cell Line, Cytoskeleton metabolism, Humans, Immunoprecipitation, Mice, Microscopy, Fluorescence, Nonmuscle Myosin Type IIA metabolism, Phosphorylation, Phosphotransferases metabolism, Protein Serine-Threonine Kinases, Actomyosin physiology, Cell Adhesion physiology, TRPM Cation Channels metabolism
- Abstract
Actomyosin contractility regulates various cell biological processes including cytokinesis, adhesion and migration. While in lower eukaryotes, alpha-kinases control actomyosin relaxation, a similar role for mammalian alpha-kinases has yet to be established. Here, we examined whether TRPM7, a cation channel fused to an alpha-kinase, can affect actomyosin function. We demonstrate that activation of TRPM7 by bradykinin leads to a Ca(2+)- and kinase-dependent interaction with the actomyosin cytoskeleton. Moreover, TRPM7 phosphorylates the myosin IIA heavy chain. Accordingly, low overexpression of TRPM7 increases intracellular Ca2+ levels accompanied by cell spreading, adhesion and the formation of focal adhesions. Activation of TRPM7 induces the transformation of these focal adhesions into podosomes by a kinase-dependent mechanism, an effect that can be mimicked by pharmacological inhibition of myosin II. Collectively, our results demonstrate that regulation of cell adhesion by TRPM7 is the combined effect of kinase-dependent and -independent pathways on actomyosin contractility.
- Published
- 2006
- Full Text
- View/download PDF
42. The channel kinases TRPM6 and TRPM7 are functionally nonredundant.
- Author
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Schmitz C, Dorovkov MV, Zhao X, Davenport BJ, Ryazanov AG, and Perraud AL
- Subjects
- Cell Division, Cell Line, Cell Membrane metabolism, Gene Deletion, Gene Expression Regulation, Genetic Complementation Test, Homeostasis, Humans, Magnesium metabolism, Phosphorylation, Protein Serine-Threonine Kinases, TRPM Cation Channels genetics, TRPM Cation Channels metabolism
- Abstract
TRPM7 and its closest homologue, TRPM6, are the only known fusions of an ion channel pore with a kinase domain. Deletion of TRPM7 in DT40 B-lymphocytes causes growth arrest, Mg(2+) deficiency, and cell death within 24-48 h. Amazingly, in analogy to TRPM6-deficient patients who can live a normal life if provided with a Mg(2+)-rich diet, TRPM7-deficient DT40 B-lymphocytes show wild type cell growth if supplied with 5-10 mm Mg(2+) concentrations in their extracellular medium. Here we have investigated the functional relationship between TRPM6 and TRPM7. We show that TRPM7 deficiency in DT40 cells cannot be complemented by heterologously expressed TRPM6. Nevertheless, both channels can influence each other's biological activity. Our data demonstrate that TRPM6 requires TRPM7 for surface expression in HEK-293 cells and also that TRPM6 is capable of cross-phosphorylating TRPM7 as assessed using a phosphothreonine-specific antibody but not vice versa. TRPM6 and TRPM7 coexpression studies in DT40 B-cells indicate that TRPM6 can modulate TRPM7 function. In conclusion, although TRPM6 and TRPM7 are closely related and deficiency in either one of these molecules severely affects Mg(2+) homeostasis regulation, TRPM6 and TRPM7 do not appear to be functionally redundant but rather two unique and essential components of vertebrate ion homeostasis regulation.
- Published
- 2005
- Full Text
- View/download PDF
43. A TRPM7 variant shows altered sensitivity to magnesium that may contribute to the pathogenesis of two Guamanian neurodegenerative disorders.
- Author
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Hermosura MC, Nayakanti H, Dorovkov MV, Calderon FR, Ryazanov AG, Haymer DS, and Garruto RM
- Subjects
- Amino Acid Sequence, Amyotrophic Lateral Sclerosis genetics, Base Sequence, Cell Line, Electrophysiology, Fluorescent Antibody Technique, Guam, Humans, Molecular Sequence Data, Mutation, Missense genetics, Parkinsonian Disorders genetics, Protein Serine-Threonine Kinases, Sequence Analysis, DNA, Amyotrophic Lateral Sclerosis metabolism, Environment, Genetic Predisposition to Disease genetics, Magnesium metabolism, Parkinsonian Disorders metabolism, TRPM Cation Channels genetics, TRPM Cation Channels metabolism
- Abstract
Guamanian amyotrophic lateral sclerosis (ALS-G) and parkinsonism dementia (PD-G) have been epidemiologically linked to an environment severely deficient in calcium (Ca2+) and magnesium (Mg2+). Transient receptor potential melastatin 7 (TRPM7) is a bifunctional protein containing both channel and kinase domains that has been proposed to be involved in the homeostatic regulation of intracellular Ca2+, Mg2+, and trace metal ion concentration. There is evidence that TRPM7 is constitutively active and that the number of available channels is dependent on intracellular free Mg2+ levels. We found a TRPM7 variant in a subset of ALS-G and PD-G patients that produces a protein with a missense mutation, T1482I. Recombinant T1482I TRPM7 exhibits the same kinase catalytic activity as WT TRPM7. However, heterologously expressed T1482I TRPM7 produces functional channels that show an increased sensitivity to inhibition by intracellular Mg2+. Because the incidence of ALS-G and PD-G has been associated with prolonged exposure to an environment severely deficient in Ca2+ and Mg2+, we propose that this variant TRPM7 allele confers a susceptibility genotype in such an environment. This study represents an initial attempt to address the important issue of gene-environment interactions in the etiology of these diseases.
- Published
- 2005
- Full Text
- View/download PDF
44. Alpha-kinase 1, a new component in apical protein transport.
- Author
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Heine M, Cramm-Behrens CI, Ansari A, Chu HP, Ryazanov AG, Naim HY, and Jacob R
- Subjects
- Animals, COS Cells, Caco-2 Cells, Cell Membrane enzymology, Chlorocebus aethiops, Dogs, Exocytosis physiology, Humans, Isomaltose metabolism, Kidney cytology, Myosin Type I metabolism, Protein Kinase Inhibitors pharmacology, Protein Kinases genetics, Protein Transport physiology, Staurosporine pharmacology, trans-Golgi Network enzymology, Cell Polarity physiology, Epithelial Cells enzymology, Membrane Microdomains enzymology, Protein Kinases metabolism
- Abstract
A key aspect in the structure of epithelial cells is the maintenance of a polarized organization based on a highly specific sorting machinery for cargo destined for the apical or the basolateral membrane domain at the exit site of the trans-Golgi network. We could recently identify two distinct post-trans-Golgi network vesicle populations that travel along separate routes to the plasma membrane, a lipid raft-dependent and a lipid raft-independent pathway. A new component of raft-carrying apical vesicles is alpha-kinase 1 (ALPK1), which was identified in immunoisolated vesicles carrying raft-associated sucrase-isomaltase (SI). This kinase was absent from vesicles carrying raft-non-associated lactase-phlorizin hydrolase. The expression of ALPK1 increases by the time of epithelial cell differentiation, whereas the intracellular localization of ALPK1 on apical transport vesicles was confirmed by confocal analysis. A phosphorylation assay on isolated SI-carrying vesicles revealed the phosphorylation of a protein band of about 105 kDa, which could be identified as the motor protein myosin I. Finally, a specific reduction of ALPK1-expression by RNA interference results in a significant decrease in the apical delivery of SI. Taken together, our data suggest that the phosphorylation of myosin I by ALPK1 is an essential process in the apical trafficking of raft-associated SI.
- Published
- 2005
- Full Text
- View/download PDF
45. Phosphorylation of annexin I by TRPM7 channel-kinase.
- Author
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Dorovkov MV and Ryazanov AG
- Subjects
- Amino Acid Sequence, Animals, Binding Sites, Calcium chemistry, Cathepsin D pharmacology, Cell Line, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Humans, Ion Channels metabolism, Ions, Magnesium chemistry, Membrane Proteins metabolism, Mice, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Peptides chemistry, Phosphorylation, Polymerase Chain Reaction, Protein Binding, Protein Kinases metabolism, Protein Structure, Tertiary, Recombinant Proteins chemistry, Sequence Homology, Amino Acid, Serine chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, TRPM Cation Channels, Trypsin pharmacology, Annexin A1 chemistry, Ion Channels physiology, Membrane Proteins physiology, Protein Kinases physiology
- Abstract
TRPM7 is an unusual bifunctional molecule consisting of a TRP ion channel fused to a protein kinase domain. It has been shown that TRPM7 plays a key role in the regulation of intracellular magnesium homeostasis as well as in anoxic neuronal death. TRPM7 channel has been characterized using electrophysiological techniques; however, the function of the kinase domain is not known and endogenous substrates for the kinase have not been reported previously. Here we have identified annexin 1 as a substrate for TRPM7 kinase. Phosphorylation of annexin 1 by TRPM7 kinase is stimulated by Ca2+ and is dramatically increased in extracts from cells overexpressing TRPM7. Phosphorylation of annexin 1 by TRPM7 kinase occurs at a conserved serine residue (Ser5) located within the N-terminal amphipathic alpha-helix of annexin 1. The N-terminal region plays a crucial role in interaction of annexin 1 with other proteins and membranes, and therefore, phosphorylation of annexin 1 at Ser5 by TRPM7 kinase may modulate function of annexin 1.
- Published
- 2004
- Full Text
- View/download PDF
46. Alpha-kinases: analysis of the family and comparison with conventional protein kinases.
- Author
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Drennan D and Ryazanov AG
- Subjects
- Amino Acid Sequence, Animals, Binding Sites, Calcium-Calmodulin-Dependent Protein Kinases chemistry, Calcium-Calmodulin-Dependent Protein Kinases genetics, Catalytic Domain, Chimera genetics, Consensus Sequence, Humans, Ion Channels chemistry, Ion Channels genetics, Ion Channels metabolism, Membrane Proteins chemistry, Membrane Proteins genetics, Membrane Proteins metabolism, Models, Molecular, Molecular Sequence Data, Protein Kinases chemistry, Protein Kinases genetics, Protein Kinases metabolism, Protein Serine-Threonine Kinases, Protozoan Proteins, Sequence Alignment, TRPM Cation Channels, eIF-2 Kinase chemistry, eIF-2 Kinase genetics, Ion Channels classification, Membrane Proteins classification, Protein Kinases classification
- Abstract
Alpha-kinases are a recently discovered family of protein kinases that have no detectable sequence homology to conventional protein kinases (CPKs). They include elongation factor 2 kinase, Dictyostelium myosin heavy chain kinases and many other protein kinases from diverse organisms, as revealed by various genome sequencing projects. Mammals have six alpha-kinases, including two channel-kinases-novel signaling molecules that contain an alpha-kinase domain fused to an ion-channel. Analysis of all known alpha-kinase sequences reveals the presence of several highly conserved motifs. Despite the fact that alpha-kinases have no detectable sequence identity with CPKs, the recently determined three-dimensional structure of the channel-kinase TRPM7/ChaK1 kinase domain reveals that alpha-kinases have a fold very similar to CPKs. Using the structural alignment of channel-kinase TRPM7/ChaK1 with cyclic-AMP dependent kinase, the consensus motifs of alpha-kinases and CPKs were aligned and compared. Remarkably, the majority of structural elements, sequence motifs, and the position of key amino acid residues important for catalysis appear to be very similar in alpha-kinases and CPKs. Differences between alpha-kinases and CPKs, and their possible impact on substrate recognition are discussed.
- Published
- 2004
- Full Text
- View/download PDF
47. Characterization of the protein kinase activity of TRPM7/ChaK1, a protein kinase fused to the transient receptor potential ion channel.
- Author
-
Ryazanova LV, Dorovkov MV, Ansari A, and Ryazanov AG
- Subjects
- Acetophenones pharmacology, Benzopyrans pharmacology, Calcium chemistry, Calmodulin chemistry, Catalytic Domain, Cations, Chromatography, Gel, Cobalt chemistry, DNA chemistry, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Electrophysiology, Enzyme Inhibitors pharmacology, Escherichia coli metabolism, HeLa Cells, Histones chemistry, Humans, Ion Channels chemistry, Ions, Kinetics, Magnesium chemistry, Magnesium pharmacology, Membrane Proteins chemistry, Molecular Sequence Data, Myelin Basic Protein physiology, Phosphoamino Acids chemistry, Phosphorylation, Protein Kinases chemistry, Protein Serine-Threonine Kinases, Protein Structure, Tertiary, Serine chemistry, Staurosporine pharmacology, TRPM Cation Channels, Threonine chemistry, Time Factors, Zinc chemistry, Ion Channels physiology, Membrane Proteins physiology, Protein Kinases physiology
- Abstract
Channel-kinase TRPM7/ChaK1 is a member of a recently discovered family of protein kinases called alpha-kinases that display no sequence homology to conventional protein kinases. It is an unusual bifunctional protein that contains an alpha-kinase domain fused to an ion channel. The TRPM7/ChaK1 channel has been characterized using electrophysiological techniques, and recent evidence suggests that it may play a key role in the regulation of magnesium homeostasis. However, little is known about its protein kinase activity. To characterize the kinase activity of TRPM7/ChaK1, we expressed the kinase catalytic domain in bacteria. ChaK1-cat is able to undergo autophosphorylation and to phosphorylate myelin basic protein and histone H3 on serine and threonine residues. The kinase is specific for ATP and cannot use GTP as a substrate. ChaK1-cat is insensitive to staurosporine (up to 0.1 mM) but can be inhibited by rottlerin. Because the kinase domain is physically linked to an ion channel, we investigated the effect of ions on ChaK1-cat activity. The kinase requires Mg(2+) (optimum at 4-10 mM) or Mn(2+) (optimum at 3-5 mM), with activity in the presence of Mn(2+) being 2 orders of magnitude higher than in the presence of Mg(2+). Zn(2+) and Co(2+) inhibited ChaK1-cat kinase activity. Ca(2+) at concentrations up to 1 mM did not affect kinase activity. Considering intracellular ion concentrations, our results suggest that, among divalent metal ions, only Mg(2+) can directly modulate TRPM7/ChaK1 kinase activity in vivo.
- Published
- 2004
- Full Text
- View/download PDF
48. Regulation of elongation factor-2 kinase by pH.
- Author
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Dorovkov MV, Pavur KS, Petrov AN, and Ryazanov AG
- Subjects
- 3T3 Cells, Amino Acid Sequence, Animals, Calcium-Calmodulin-Dependent Protein Kinases biosynthesis, Calcium-Calmodulin-Dependent Protein Kinases genetics, Calmodulin physiology, Dictyostelium, Elongation Factor 2 Kinase, Enzyme Activation genetics, Enzyme Activation physiology, Fibroblasts enzymology, Fibroblasts metabolism, Hydrogen-Ion Concentration, Intracellular Fluid enzymology, Kinetics, Liver enzymology, Liver metabolism, Mice, Molecular Sequence Data, Peptide Elongation Factor 2 metabolism, Peptide Fragments chemical synthesis, Peptide Fragments metabolism, Phosphorylation, Protein Biosynthesis, Rabbits, Recombinant Fusion Proteins metabolism, Transfection, Calcium-Calmodulin-Dependent Protein Kinases metabolism, Intracellular Fluid physiology
- Abstract
Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that phosphorylates and inactivates eEF-2 and that can regulate the rate of protein synthesis at the elongation stage. Here we report that a slight decrease in pH, within the range observed in vivo, leads to a dramatic activation of eEF-2K. The activity of eEF-2K in mouse liver extracts, as well as the activity of purified recombinant human eEF-2K, is low at pH 7.2-7.4 and is increased by severalfold when the pH drops to 6.6-6.8. eEF-2K requires calmodulin for activity at neutral as well as acidic pH. Kinetic studies demonstrate that the pH does not affect the K(M) for ATP or eEF-2 and activation of eEF-2K at acidic pH is due to an increase in V(max). To analyze the potential role of eEF-2K in regulating protein synthesis by pH, we constructed a mouse fibroblast cell line that expresses eEF-2K in a tetracycline-regulated manner. Overexpression of eEF-2K led to a decreased rate of protein synthesis at acidic pH, but not at neutral pH. Our results suggest that pH-dependent activation of eEF-2K may play a role in the global inhibition of protein synthesis during tissue acidosis, which accompanies such processes as hypoxia and ischemia.
- Published
- 2002
- Full Text
- View/download PDF
49. Elongation factor-2 kinase and its newly discovered relatives.
- Author
-
Ryazanov AG
- Subjects
- Animals, Calcium-Calmodulin-Dependent Protein Kinases classification, Elongation Factor 2 Kinase, Hydrogen-Ion Concentration, Mammals, Peptide Elongation Factor 2 metabolism, Phosphorylation, Phylogeny, Calcium-Calmodulin-Dependent Protein Kinases metabolism
- Abstract
Phosphorylation of elongation factor-2 (eEF-2) by the highly specific eEF-2 kinase results in eEF-2 inactivation and, therefore, may regulate the global rate of protein synthesis in animal cells. Cloning and sequencing of eEF-2 kinase led to the discovery of a new family of protein kinases, named alpha-kinases, whose catalytic domains display no sequence homology to conventional eukaryotic protein kinases. Several mammalian alpha-kinases have recently been cloned. Two of these alpha-kinases, named channel-kinases 1 and 2 (ChaK1 and ChaK2) represent a new type of signaling molecules that are protein kinases fused to ion channels.
- Published
- 2002
- Full Text
- View/download PDF
50. Protein turnover plays a key role in aging.
- Author
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Ryazanov AG and Nefsky BS
- Subjects
- Animals, Humans, Aging metabolism, Proteins metabolism
- Abstract
Although the molecular mechanism of aging is unknown, a progressive increase with age in the concentration of damaged macromolecules, especially proteins, is likely to play a central role in senescent decline. In this paper, we discuss evidence that the progressive decrease in protein synthesis and turnover can be the primary cause of the increase in the concentration of damaged proteins with age. Conversely, protein damage itself is likely to be the cause of the decrease in protein turnover. This could establish a positive feedback loop where the increase in protein damage decreases the protein turnover rate, leading to a further increase in the concentration of damaged proteins. The establishment of such a feedback loop should result in an exponential increase in the amount of protein damage-a protein damage catastrophe-that could be the basis of the general deterioration observed in senescent organisms.
- Published
- 2002
- Full Text
- View/download PDF
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