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Institutions have been slow to respond to calls from Indigenous nations, organizations, and scholars to require free, prior, and informed consent before authorizing use of their cultural heritage materials in publications, exhibition, and research. In the United States, the Native American Graves Protection and Repatriation Act of 1990 fundamentally changed the relationship between museums, archaeologists, and Indigenous nations, requiring institutions to inventory their collections and consult with descendant communities on repatriation of specific Indigenous collections. In response, institutions and their personnel have come to view Indigenous collections as those subject to NAGPRA and those that are not—NAGPRA/Not NAGPRA. Many Indigenous nations, however, do not accept this demarcation, resulting in continued frustration and trauma for those descendant communities. This case study follows the evolving relationship between the White Earth Band of the Minnesota Chippewa Tribe and the Robert S. Peabody Institute of Archaeology. Beginning with repatriation, the relationship has expanded to consider how the museum and Indigenous nation can collaborate on the care and curation of cultural heritage materials that remain at the Peabody Institute. Most recently, White Earth and the Peabody have executed an MOU that governs how the museum will handle new acquisitions, found-in-collections materials, and donor offers. The relationship with the White Earth also has influenced how the Peabody Institute approaches its holdings of Indigenous cultural heritage materials more broadly, blurring the line between NAGPRA and Not NAGPRA collections. The Peabody Institute is working to revise its collections policy to require free, prior, and informed consent prior to use of Indigenous cultural heritage materials in publications, exhibitions, and research.

Understanding the causes and consequences of previous climate changes is essential for testing present-day climate models and projections. Archaeological sites are paleoenvironmental archives containing unique ecological baselines with data on paleoclimate transformations at a human timescale. Anthropogenic and nonanthropogenic forces have destroyed many sites, and others are under immediate threat. In the face of this loss, previously excavated collections from these sites—referred to as legacy collections—offer a source of climate and other paleoenvironmental information that may no longer exist elsewhere. Here, we 1) review obstacles to systematically using data from legacy archaeological collections, such as inconsistent or unreported field methods, inadequate records, unsatisfactory curation, and insufficient public knowledge of relevant collections; 2) suggest best practices for integrating archaeological data into climate and environmental research; and 3) summarize several studies to demonstrate the benefits and challenges of using legacy collections as archives of local and regional environmental proxies. Data from archaeological legacy collections contribute regional ecological baselines as well as serve to correct shifting baselines. They also enable regional climate reconstructions at various timescales and corroborate or refine radiocarbon dates. Such uses of legacy collections raise ethical concerns regarding ownership of and responsibility for cultural resources and highlight the importance of Indigenous involvement in planning and executing fieldwork and stewardship of cultural heritage. Finally, we discuss methodologies, practices, and policies pertaining to archaeological legacy collections and support calls for discipline-wide shifts in collections management to ensure their long-term utility in multidisciplinary research and public engagement.

Most major cities worldwide are affected Urban Heat Islands – a condition of relatively higher temperatures being observed in one area compared to another that can be caused by a decrease in greens...

Natural products are produced by bacteria, plants, and fungi and have yielded some of the most clinically active and widely used drugs available. Several of the natural products that are produced by type II PKSs have novel scaffolds or unique rearrangements that are, in large part, due to the post PKS enzymes. Protein–protein interactions between post-PKS tailoring enzymes hamper the use of combinatorial biosynthesis in the development of novel natural product-derived drugs. Several co-dependent post-PKS enzymes have been characterized in nature, but their significance in regards to complex formation and co-dependence has been largely overlooked. Here, we report an in-depth analysis of two such post-PKS pathways, gilvocarcin and mithramycin, for which indications exist to postulate multienzyme complexes to facilitate substrate protection and channeling.

Incorporating career development into PhD course offerings can help biomedical students better understand themselves, as well as the job market, and to adopt a 'can-do' attitude in developing their own paths.

MtmOIV and MtmW catalyze the final two reactions in the mithramycin (MTM) biosynthetic pathway, the Baeyer-Villiger opening of the fourth ring of premithramycin B (PMB), creating the C3 pentyl side chain, strictly followed by reduction of the distal keto group on the new side chain. Unexpectedly this results in a C2 stereoisomer of mithramycin, iso-mithramycin (iso-MTM). Iso-MTM undergoes a non-enzymatic isomerization to MTM catalyzed by Mg(2+) ions. Crystal structures of MtmW and its complexes with co-substrate NADPH and PEG, suggest a catalytic mechanism of MtmW. The structures also show that a tetrameric assembly of this enzyme strikingly resembles of the ring-shaped β subunit of a vertebrate ion channel. We show that MtmW and MmOIV form a complex in the presence of PMB and NADPH, presumably to hand over the unstable MtmOIV product to MtmW, yielding iso-MTM, as a potential self-resistance mechanism against MTM toxicity.

This chapter explores the context of the three monumental animal and bird carvings found at the Hontoon/Thursby site on the St. Johns River: the owl, otter, and pelican. Archaeological and ethnographic information from Florida and surrounding areas is brought to play in interpreting the carvings and their placement at the site.

A suite of scientific approaches are applied to four pre- Columbian wood sculptures from east-central Florida, comprising new radiocarbon determinations, wood identification and strontium isotope analysis. The dates for three large zoomorphic carvings recovered from the St. Johns River at Hontoon Island place them between ca. AD 1300 and 1600, suggesting that they belonged to a tradition of erecting largescale pine carvings spanning at least some centuries. Two of the carvings have strontium isotope signals consistent with the immediate vicinity of the site, while the third differs significantly. Baseline data for biologically available strontium from sampled modern trees indicates considerable isotopic variability over short distances, making it difficult to determine the source of the wood used for this third carving. The only anthropomorphic sculpture, recovered from the vicinity of Tomoka State Park, dates to a similar time period, ca. AD 1440-1620. Our study confirms the wood's previous identification as belonging to the genus Peltophorum, a tropical hardwood thought not to be native to Florida. Its strontium isotope value is consistent with its find location, but equally may be found over much of southern Florida, where perhaps the species grew in the past. The results clarify the chronology for a stylistically distinctive carving tradition, as well as raising questions concerning the exchange of organic materials over varying distances. Highlights • 14C results for four east-central Florida carvings (Hontoon Island; Tomoka State Park) range ca. AD 1300-1600, spanning the proto-historic/historic periods • 87Sr/86Sr results for two of the three Hontoon carvings are consistent with the immediate locale, while the third suggests a different provenance • Pinus sp. was used at Hontoon, while Peltophorum sp., currently not native to Florida, was used at Tomoka

Halobacteria require high NaCl concentrations for growth and are the dominant inhabitants of hypersaline environments above 15% NaCl. They are well-documented to be highly recombinogenic, both in frequency and in the range of exchange partners. In this study, we examine the genetic and genomic variation of cultured, naturally co-occurring environmental populations of Halobacteria. Sequence data from multiple loci (~2500 bp) identified many closely and more distantly related strains belonging to the genera Halorubrum and Haloarcula. Genome fingerprinting using a random priming PCR amplification method to analyze these isolates revealed diverse banding patterns across each of the genera and surprisingly even for isolates that are identical at the nucleotide level for five protein coding sequenced loci. This variance in genome structure even between identical multilocus sequence analysis (MLSA) haplotypes indicates that accumulation of genomic variation is rapid: faster than the rate of third codon substitutions.

The Halobacteria are known to engage in frequent gene transfer and homologous recombination. For stably diverged lineages to persist some checks on the rate of between lineage recombination must exist. We surveyed a group of isolates from the Aran-Bidgol endorheic lake in Iran and sequenced a selection of them. Multilocus Sequence Analysis (MLSA) and Average Nucleotide Identity (ANI) revealed multiple clusters (phylogroups) of organisms present in the lake. Patterns of intein and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) presence/absence and their sequence similarity, GC usage along with the ANI and the identities of the genes used in the MLSA revealed that two of these clusters share an exchange bias toward others in their phylogroup while showing reduced rates of exchange with other organisms in the environment. However, a third cluster, composed in part of named species from other areas of central Asia, displayed many indications of variability in exchange partners, from within the lake as well as outside the lake. We conclude that barriers to gene exchange exist between the two purely Aran-Bidgol phylogroups, and that the third cluster with members from other regions is not a single population and likely reflects an amalgamation of several populations.

The past decade has witnessed the popularity of push-based data management systems, in which the query executor passively receives data from either remote data sources (e.g., sensors) or I/O processes that scan database tables/files from local storage. Unlike traditional relational database management system (RDBMS) architectures that are mostly I/O-bound, push-based database systems often become heavily computation-bound since the data arrival rate could be very high. In this paper, we argue that modern multi-core hardware, especially Graphics Processing Units (GPU), provide the most cost-effective computing platform to catch up with the large amount of data streamed into a push-based database system. Based on that, we will open discussions on how to design and implement a query processing engine for such systems that run on GPUs.