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9 results on '"Ryan C. Hutchinson"'

1. A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra

Author

Gervaise H. Henry, Alicia Malewska, Diya B. Joseph, Venkat S. Malladi, Jeon Lee, Jose Torrealba, Ryan J. Mauck, Jeffrey C. Gahan, Ganesh V. Raj, Claus G. Roehrborn, Gary C. Hon, Malcolm P. MacConmara, Jeffrey C. Reese, Ryan C. Hutchinson, Chad M. Vezina, and Douglas W. Strand

Subjects
Biology (General), QH301-705.5
Abstract

Summary: A comprehensive cellular anatomy of normal human prostate is essential for solving the cellular origins of benign prostatic hyperplasia and prostate cancer. The tools used to analyze the contribution of individual cell types are not robust. We provide a cellular atlas of the young adult human prostate and prostatic urethra using an iterative process of single-cell RNA sequencing (scRNA-seq) and flow cytometry on ∼98,000 cells taken from different anatomical regions. Immunohistochemistry with newly derived cell type-specific markers revealed the distribution of each epithelial and stromal cell type on whole mounts, revising our understanding of zonal anatomy. Based on discovered cell surface markers, flow cytometry antibody panels were designed to improve the purification of each cell type, with each gate confirmed by scRNA-seq. The molecular classification, anatomical distribution, and purification tools for each cell type in the human prostate create a powerful resource for experimental design in human prostate disease. : Using single-cell RNA sequencing, immunofluorescence, and flow cytometry, Henry et al. create a cellular anatomy of the normal human prostate and provide the tools to identify, isolate, and localize every cell type. They identify two additional epithelial cell types enriched in the prostatic urethra and proximal prostatic ducts. Keywords: human prostate, zonal anatomy, single-cell RNA sequencing, flow cytometry, prostate cancer, benign prostatic hyperplasia, human cell atlas, prostate epithelia, prostate stroma, GUDMAP

Published
2018
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2. Magnetic resonance imaging to detect vesico-symphyseal fistula following robotic prostatectomy

Author

Ryan C. Hutchinson, David D. Thiel, and Joseph M. Bestic

Subjects
Diseases of the genitourinary system. Urology, RC870-923
Abstract

Pubic complications following radical prostatectomy are rare. Osteitis pubis typically presents with symptoms related to irritation of the pubic rami including pain with ambulation and adduction of the leg. A 60-year-old male with prostatic adenocarcinoma underwent uneventful robotic assisted laparoscopic prostatectomy. The patient noted the onset of severe pubic pain exacerbated by ambulation approximately one month post-surgery. An abdominal/pelvic CT scan was negative for acute pathology. Due to continued discomfort, a multiplanar MRI of the pelvis was performed with and without intravenous contrast material (20 ml Omniscan). The MRI demonstrated irregularity of the bladder base and proximal urethra with a fistulous tract extending anteriorly in direct communication with the pubic symphysis joint space. Vague periarticular marrow edema-like signal and enhancement at the pubic symphysis was thought to represent osteitis pubis. The patient's symptoms resolved after one month of urethral catheter drainage, intravenous antibiotics, and anti-inflammatory therapy.

Published
2013

3. Erosion of inferior vena caval filter noted during robotic assisted laparoscopic partial nephrectomy

Author

Ryan C. Hutchinson, David D. Thiel, and Todd C. Igel

Subjects
Diseases of the genitourinary system. Urology, RC870-923
Abstract

Inferior Vena Cava (IVC) filters are mechanical devices implanted to provide prophylaxis against pulmonary emboli in patients for whom standard anticoagulation is either inadequate or contraindicated. A 67-year-old female with a 10-year-old indwelling IVC filter underwent robotic assisted laparoscopic partial nephrectomy for a right upper pole renal mass. Renal hilum dissection was complicated by adhesions secondary to eroded IVC filter struts. IVC filter erosion is a well-described phoenomena in both the radiologic and surgical literature. As many as 25% of filters are noted to be radiographically eroded; however, the incidence of clinically significant erosion is much less. Given the placement of endovascularly delivered IVC filters in close proximity to many urologic operative fields, it is important for urologists to be aware of the potential of eroded devices when pursuing para-caval dissections.

Published
2012
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4. Prostatic Stromal Hyperplasia with Atypia

Author

Ryan C. Hutchinson, Kevin J. Wu, John C. Cheville, and David D. Thiel

Subjects
Diseases of the genitourinary system. Urology, RC870-923
Abstract

Prostatic stromal hyperplasia with atypia (PSHA) is a rare histologic finding diagnosed incidentally on prostate biopsies, transurethral resection specimens, and radical prostatectomy specimens. PSHA has a bizarre histologic appearance and these lesions often raise concern for sarcoma; however, their clinical course is indolent and does not include extraprostatic progression. We discuss a case of PHSA discovered on prostate biopsy performed for an abnormal digital rectal examination and review the literature on this rare pathologic finding.

Published
2013
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5. 5-alpha reductase inhibitors induce a prostate luminal to club cell transition in human benign prostatic hyperplasia

Author

Diya B Joseph, Gervaise H Henry, Alicia Malewska, Jeffrey C Reese, Ryan J Mauck, Jeffrey C Gahan, Ryan C Hutchinson, James L Mohler, Claus G Roehrborn, and Douglas W Strand

Subjects
Male, 5-alpha Reductase Inhibitors, Lower Urinary Tract Symptoms, Prostate, Prostatic Hyperplasia, Humans, Dihydrotestosterone, Atrophy, Article, Pathology and Forensic Medicine
Abstract

Benign prostatic hyperplasia (BPH) is a progressive expansion of peri-urethral prostate tissue common in aging men. Patients with enlarged prostates are treated with 5-alpha reductase inhibitors (5ARIs) to shrink prostate volume by blocking the conversion of testosterone to dihydrotestosterone (DHT). A reduction in DHT levels can elicit atrophy and apoptosis of prostate secretory luminal cells, which results in a favorable clinical response characterized by improved lower urinary tract symptoms. However, the histologic response to 5ARI treatment is often heterogeneous across prostate acini and lower urinary tract symptoms can persist to require surgical intervention. We used two spatial profiling approaches to characterize gene expression changes across histologically normal and atrophied regions in prostates from 5ARI-treated men. Objective transcriptomic profiling using the Visium spatial gene expression platform showed that 5ARI-induced atrophy of prostate luminal cells correlated with reduced androgen receptor signaling and increased expression of urethral club cell genes including LTF, PIGR, OLFM4, SCGB1A1, and SCGB3A1. Prostate luminal cells within atrophied acini adapted to decreased DHT conditions by increasing NF-κB signaling and anti-apoptotic BCL2 expression, which may explain their survival. Using GeoMx digital spatial profiling with a probe set to assess ~18 000 RNA targets, we confirmed that atrophied acini expressing SCGB3A1 displayed higher levels of club cell markers compared with histologically normal acini with NKX3-1 expression. In addition, club-like cells within regions of 5ARI-induced atrophy closely resembled true club cells from the prostatic urethra. A comparison of histologically normal regions from 5ARI-treated men and histologically normal regions from untreated men revealed few transcriptional differences. Taken together, our results describe a heterogeneous response to 5ARI treatment where cells in atrophied acini undergo an adaptation from a prostate secretory luminal to a club cell-like state in response to 5ARI treatment. © 2021 The Pathological Society of Great Britain and Ireland.

Published
2022

7. Urothelial cancer of the urinary bladder: can lessons learned be applied to the upper urinary tract?

Author

Laura M, Krabbe, Ryan C, Hutchinson, and Vitaly, Margulis

Subjects
Carcinoma, Transitional Cell, Urologic Neoplasms, Urinary Bladder Neoplasms, Humans, Combined Modality Therapy
Abstract

Even though urothelial cancer may occur anywhere in the urinary tract, it is most commonly found in the urinary bladder. Due to its higher incidence, this disease is studied in the bladder much more frequently than in the upper urinary tract. The question that arises is, to what extent can concepts and treatment paradigms derived from lower tract disease be applied to urothelial carcinoma of the upper urinary tract? This review aims at providing an overview of established care concepts in urothelial carcinoma of the bladder and applicability of these findings to tumors of the upper urinary tract.

Published
2016

8. Optimizing oncologic outcomes in upper tract urothelial carcinoma

Author

Ryan C, Hutchinson and Vitaly, Margulis

Subjects
Carcinoma, Transitional Cell, Urologic Neoplasms, Treatment Outcome, Humans, Urologic Surgical Procedures, Prognosis, Combined Modality Therapy, Neoadjuvant Therapy
Abstract

Upper tract urothelial carcinoma is a disease with rapidly changing management. Though rare, recent multi-institutional collaborations have allowed for study of its biology and treatment outcomes in greater detail than ever before. In coming years physicians treating ureteral cancers will have an expanded armamentarium of treatment options and better data on which to base treatment decisions. Currently there is exciting work underway both in terms of developmental therapies, including neoadjuvant chemotherapy, as well as improved prognostics allowing for better utilization of nephron-sparing approaches where applicable. We present a review of current management techniques and the data on which to base management choices for surgeons treating upper tract urothelial carcinoma. The ultimate goal is improving outcomes for patients; with recent developments and current work in the field, there is reason to be optimistic for the future in this rare, challenging disease.

Published
2016

9. Contributors

Author

Ross A. Abrams, David J. Adelstein, Kaled M. Alektiar, Brian Alexander, Jan Alsner, Ersan Altun, Bethany Anderson, K. Kian Ang, Douglas W. Arthur, Jonathan B. Ashman, Matthew T. Ballo, Christopher Andrew Barker, Beth M. Beadle, Phillipe Bedard, Jonathan J. Beitler, Michael W. Bishop, A. William Blackstock, Jeffrey A. Bogart, James A. Bonner, J. Daniel Bourland, Joseph Bovi, John Breneman, Juan P. Brito, Paul D. Brown, Michael D. Brundage, Thomas A. Buchholz, Bryan Henry Burmeister, Stuart K. Calderwood, Matthew D. Callister, Felipe A. Calvo, George M. Cannon, Bruce A. Chabner, Michael D. Chan, Sam T. Chao, Anne-Marie Charpentier, Christine H. Chung, Peter W.M. Chung, Louis S. Constine, Benjamin W. Corn, Allan Covens, Oana I. Craciunescu, Christopher H. Crane, Carien L. Creutzberg, Juanita M. Crook, Walter J. Curran, Brian G. Czito, Bouthaina S. Dabaja, Shiva Das, Marc David, Laura A. Dawson, Thomas F. DeLaney, Phillip M. Devlin, Mark Dewhirst, Don S. Dizon, Jeffrey S. Dome, John H. Donohue, Thierry P. Duprez, Jason A. Efstathiou, Avraham Eisbruch, David W. Eisele, Mary Feng, Rui P. Fernandes, Julia R. Fielding, Gini F. Fleming, Robert L. Foote, Benedick A. Fraass, Carolyn R. Freeman, Adam S. Garden, Lindell R. Gentry, Lilian T. Gien, Mary K. Gospodarowicz, Cai Grau, Vincent Grégoire, Craig M. Greven, Kathryn McConnell Greven, Leonard L. Gunderson, Michael G. Haddock, Michele Halyard, Marc Hamoir, Timothy Paul Hanna, Paul M. Harari, Ian D. Hay, Joseph M. Herman, Caroline L. Holloway, Theodore Sunki Hong, Neil S. Horowitz, Michael R. Horseman, Julie Howle, Brian A. Hrycushko, David Hsu, Patricia A. Hudgins, Ryan C. Hutchinson, Christine Iacobuzio-Donahue, Benjamin Izar, Valerie L. Jewells, Joseph Gerard Jurcic, John A. Kalapurakal, Brian D. Kavanagh, Kara M. Kelly, Amir H. Khandani, Deepak Khuntia, Ana Ponce Kiess, Susan J. Knox, Wui-Jin Koh, Matthew J. Krasin, Larry E. Kun, Nadia Issa Laack, Ann S. LaCasce, Corey Jay Langer, George E. Laramore, Andrew B. Lassman, Colleen A.F. Lawton, Nancy Lee, Benoît Lengelé, William P. Levin, Jacob C. Lindegaard, John T. Lucas, Shannon M. MacDonald, William J. Mackillop, Anuj Mahindra, Anthony A. Mancuso, Karen Jean Marcus, Lawrence B. Marks, Diana Matceyevsky, Jean-Jacques Mazeron, Mark W. McDonald, Paul M. Medin, Minesh P. Mehta, William M. Mendenhall, Ruby F. Meredith, Jeff M. Michalski, Michael T. Milano, Bruce D. Minsky, William H. Morrison, Erin S. Murphy, Rashmi K. Murthy, Andrea K. Ng, Marianne Nordsmark, Desmond A. O'Farrell, Paul Okunieff, Roger Ove, Jens Overgaard, Manisha Palta, Alexander S. Parker, Luke E. Pater, Jennifer L. Peterson, Thomas M. Pisansky, Louis Potters, Harry Quon, David Raben, Abram Recht, Ramesh Rengan, Marsha, Laufer Reyngold, Nadeem Riaz, Stephen S. Roberts, Kenneth B. Roberts, Jason K. Rockhill, Claus M. Rödel, Carlos Rodriguez-Galindo, C. Leland Rogers, Todd L. Rosenblat, William G. Rule, Anthony Henryk Russell, Suzanne Russo, David P. Ryan, John Torsten Sandlund, Pamela L. Sandow, Daniel J. Sargent, Steven E. Schild, Michael Heinrich Seegenschmiedt, Chirag Shah, Edward G. Shaw, Jason P. Sheehan, Arif Sheikh, Qian Shi, Malika L. Siker, William Small, Benjamin D. Smith, Grace L. Smith, Timothy D. Solberg, Paul R. Stauffer, Mary Ann Stevenson, Alexandra J. Stewart, John H. Suh, Winston W. Tan, Joel E. Tepper, Charles R. Thomas, Gillian M. Thomas, Robert D. Timmerman, Richard W. Tsang, Kenneth Y. Usuki, Vincenzo Valentini, Vicente Valero, Martin J. van den Bent, Michael J. Veness, Frank A. Vicini, Danielle Vicus, Akila N. Viswanathan, Zeljko Vujaskovic, J. Trad Wadsworth, Henry Wagner, Daniel R. Wahl, Padraig R. Warde, Timothy V. Waxweiler, Michael J. Wehle, Robert J. Weil, Lawrence M. Weiss, John W. Werning, Christopher G. Willett, Christopher Douglas Willey, Lynn D. Wilson, Karen M. Winkfield, Jennifer Yon-Li, Suzanne L. Wolden, Terence Z. Wong, Jeffrey Y.C. Wong, William W. Wong, Wenting Wu, Joachim Yahalom, Eddy Shih-Hsin Yang, Y. Nancy You, Elaine M. Zeman, Jing Zeng, and Anthony L. Zietman

Published
2016
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