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Studying the specific and intraspecific structure of populations of causative agents of Fusarium ear of grain crops. The results of experimental studies of samples of grain crops affected by microscopic fungi p.Fusarium. The analysis of the infection of grains, stems and roots of various types of cereals with mitospores fungi was carried out. The variability of pathogenic properties of Fusarium fungi depending on the part of the ear is shown. The results of the research are important for the targeted diagnosis of fusarium of grain crops and plants of the ear.

Paper presents the developed algorithm for multi-carrier simultaneous vertical ionospheric sounding by OFDM signal with BPSK modulation of subcarriers. There is presented a mathematical model, numerical and simulation modeling with the use of USRP platform. Research findings proved the fundamental feasibility of using orthogonal subcarriers for simultaneous sounding of channels over a wide band.

A comparative analysis of variations in the total electron content of the Earth’s ionosphere over the territory of ten experimental stations located in the Volga region from December 2015 to November 2016 was carried out, taking into account the influence of geographic latitude and level of ionospheric disturbance.

The number of infections caused by antibiotic-resistant strains of bacteria is growing by the year. The pathogenic bacterial species Enterococcus faecalis and Enterococcus faecium are among the high priority candidate targets for the development of new therapeutic antibacterial agents. One of the most promising antibacterial agents are bacteriophages. According to the WHO, two phage-based therapeutic cocktails and two medical drugs based on phage endolysins are currently undergoing clinical trials. In this paper, we describe the virulent bacteriophage iF6 and the properties of two of its endolysins. The chromosome of the iF6 phage is 156,592 bp long and contains two direct terminal repeats, each 2108 bp long. Phylogenetically, iF6 belongs to the Schiekvirus genus, whose representatives are described as phages with a high therapeutic potential. The phage demonstrated a high adsorption rate; about 90% of iF6 virions attached to the host cells within one minute after the phage was added. Two iF6 endolysins were able to lyse enterococci cultures in both logarithmic and stationary growth phases. Especially promising is the HU-Gp84 endolysin; it was active against 77% of enterococci strains tested and remained active even after 1 h incubation at 60 °C. Thus, iF6-like enterococci phages appear to be a promising platform for the selection and development of new candidates for phage therapy. [ABSTRACT FROM AUTHOR]

Cytotoxicity of amyloid fibrils has been shown to depend on their structure. However, specific features of toxic and non-toxic amyloids remain unclear. Here we focus on the relationship between structural characteristics of the fibrils and their cytotoxicity. Bovine carbonic anhydrase B (BCAB) serves as the object of this study because its amyloids reduce cell viability. Limited proteolysis and mass spectrometry were used to determine BCAB regions forming the core of amyloid fibrils. Four BCAB mutants with substitutions reducing hydrophobicity in the regions important for amyloid formation were obtained to study the kinetics of aggregation, structural features, and cytotoxicity of the amyloids. We demonstrate that fibrils of WT BCAB, L78A, L139A, and M239A variants display a pronounced toxic effect on eukaryotic cells, while I208A mutation significantly reduces the cell-damaging effect of amyloids. The data obtained conclude that cytotoxicity of BCAB fibrils does not depend on their length, secondary structure, and exposure of hydrophobic groups to the solvent. A distinctive feature of the low-toxic I208A fibrils is their specific morphology characterized by the lateral protofilaments association and formation of fibril-ribbons. • Amyloid fibrils of BCAB and its mutants decrease eukaryotic cells viability. • The fibrils cytotoxicity doesn't depend on their length, secondary structure, and ANS binding. • Specific feature of the low-toxic amyloids is the lateral protofilaments association. [ABSTRACT FROM AUTHOR]

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