90 results on '"Ruyong Yao"'
Search Results
2. Anti-herpes simplex virus activities and mechanisms of marine derived compounds
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Cui Hao, Zhongqiu Xu, Can Xu, and Ruyong Yao
- Subjects
marine compound ,herpes simplex virus ,antiviral activity ,molecular mechanism ,therapeutic application ,Microbiology ,QR1-502 - Abstract
Herpes simplex virus (HSV) is the most widely prevalent herpes virus worldwide, and the herpetic encephalitis and genital herpes caused by HSV infection have caused serious harm to human health all over the world. Although many anti-HSV drugs such as nucleoside analogues have been ap-proved for clinical use during the past few decades, important issues, such as drug resistance, toxicity, and high cost of drugs, remain unresolved. Recently, the studies on the anti-HSV activities of marine natural products, such as marine polysaccharides, marine peptides and microbial secondary metabolites are attracting more and more attention all over the world. This review discusses the recent progress in research on the anti-HSV activities of these natural compounds obtained from marine organisms, relating to their structural features and the structure-activity relationships. In addition, the recent findings on the different anti-HSV mechanisms and molecular targets of marine compounds and their potential for therapeutic application will also be summarized in detail.
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- 2024
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3. SIRT1-dependent mitochondrial biogenesis supports therapeutic effects of vidarabine against rotenone-induced neural cell injury
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Lanxin Li, Yang Zhang, Zhengqian Chen, Ruyong Yao, Zhongqiu Xu, Can Xu, Fujie He, Haitao Pei, and Cui Hao
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Parkinson's disease ,SH-SY5Y cells ,mitochondrial biogenesis ,oxidative stress ,SIRT1 ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Parkinson's disease (PD) is the second most common neurodegenerative disease in the world, which is distinguished by the loss of dopaminergic (DA) neurons in the substantia nigra and the formation of intraneuronal. Numerous studies showed that the damage and dysfunction of mitochondria may play key roles in DA neuronal loss. Thus, it is necessary to seek therapeutic measures for PD targeting mitochondrial function and biogenesis. In this study, through screening the purchased compound library, we found that marine derived vidarabine had significant neuroprotective effects against rotenone (ROT) induced SH-SY5Y cell injury. Further studies indicated that vidarabine pretreatment significantly protected ROT-treated SH-SY5Y cells from toxicity by preserving mitochondrial morphology, improving mitochondrial function, and reducing cell apoptosis. Vidarabine also reduced the oxidative stress and increased the expression levels of PGC-1α, NRF1, and TFAM proteins, which was accompanied by the increased mitochondrial biogenesis. However, the neuroprotective effects of vidarabine were counteracted in the presence of SIRT1-specific inhibitor Ex-527. Besides, vidarabine treatment attenuated the weight loss, alleviated the motor deficits and inhibited the neuronal injury in the MPTP induced mouse model. Thus, vidarabine may exert neuroprotective effects via a mechanism involving specific connections between the SIRT1-dependent mitochondrial biogenesis and its antioxidant capacity, suggesting that vidarabine has potential to be developed into a novel therapeutic agent for PD.
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- 2023
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4. A dynamics association study of gut barrier and microbiota in hyperuricemia
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Qiulan Lv, Jun Zhou, Changyao Wang, Xiaomin Yang, Yafei Han, Quan Zhou, Ruyong Yao, and Aihua Sui
- Subjects
hyperuricemia ,gut microbiota ,intestinal barrier ,dynamic changes ,dyslipidemia ,Microbiology ,QR1-502 - Abstract
IntroductionThe intricate interplay between gut microbiota and hyperuricemia remains a subject of growing interest. However, existing studies only provided snapshots of the gut microbiome at single time points, the temporal dynamics of gut microbiota alterations during hyperuricemia progression and the intricate interplay between the gut barrier and microbiota remain underexplored. Our investigation revealed compelling insights into the dynamic changes in both gut microbiota and intestinal barrier function throughout the course of hyperuricemia.MethodsThe hyperuricemia mice (HY) were given intragastric administration of adenine and potassium oxalate. Gut microbiota was analyzed by 16S rRNA sequencing at 3, 7, 14, and 21 days after the start of the modeling process. Intestinal permeability as well as LPS, TNF-α, and IL-1β levels were measured at 3, 7, 14, and 21 days.ResultsWe discovered that shifts in microbial community composition occur prior to the onset of hyperuricemia, key bacterial Bacteroidaceae, Bacteroides, and Blautia exhibited reduced levels, potentially fueling microbial dysbiosis as the disease progresses. During the course of hyperuricemia, the dynamic fluctuations in both uric acid levels and intestinal barrier function was accompanied with the depletion of key beneficial bacteria, including Prevotellaceae, Muribaculum, Parabacteroides, Akkermansia, and Bacteroides, and coincided with an increase in pathogenic bacteria such as Oscillibacter and Ruminiclostridium. This microbial community shift likely contributed to elevated lipopolysaccharide (LPS) and pro-inflammatory cytokine levels, ultimately promoting metabolic inflammation. The decline of Burkholderiaceae and Parasutterella was inversely related to uric acid levels, Conversely, key families Ruminococcaceae, Family_XIII, genera Anaeroplasma exhibited positive correlations with uric acid levels. Akkermansiaceae and Bacteroidaceae demonstrating negative correlations, while LPS-containing microbiota such as Desulfovibrio and Enterorhabdus exhibited positive correlations with intestinal permeability.ConclusionIn summary, this study offers a dynamic perspective on the complex interplay between gut microbiota, uric acid levels, and intestinal barrier function during hyperuricemia progression. Our study suggested that Ruminiclostridium, Bacteroides, Akkermansiaceae, Bilophila, Burkholderiaceae and Parasutterella were the key bacteria that play vital rols in the progress of hyperuricemia and compromised intestinal barrier, which provide a potential avenue for therapeutic interventions in hyperuricemia.
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- 2023
- Full Text
- View/download PDF
5. The role and mechanisms of gut microbiota in diabetic nephropathy, diabetic retinopathy and cardiovascular diseases
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Qiulan Lv, Zhiyuan Li, Aihua Sui, Xiaomin Yang, Yafei Han, and Ruyong Yao
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T2DM-related complications ,gut microbiota ,microbial metabolites ,intestinal barrier ,immunity ,microbiological therapy ,Microbiology ,QR1-502 - Abstract
Type 2 diabetes mellitus (T2DM) and T2DM-related complications [such as retinopathy, nephropathy, and cardiovascular diseases (CVDs)] are the most prevalent metabolic diseases. Intriguingly, overwhelming findings have shown a strong association of the gut microbiome with the etiology of these diseases, including the role of aberrant gut bacterial metabolites, increased intestinal permeability, and pathogenic immune function affecting host metabolism. Thus, deciphering the specific microbiota, metabolites, and the related mechanisms to T2DM-related complications by combined analyses of metagenomics and metabolomics data can lead to an innovative strategy for the treatment of these diseases. Accordingly, this review highlights the advanced knowledge about the characteristics of the gut microbiota in T2DM-related complications and how it can be associated with the pathogenesis of these diseases. Also, recent studies providing a new perspective on microbiota-targeted therapies are included.
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- 2022
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6. Prognostic value of eight immune gene signatures in pancreatic cancer patients
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Wenting Wang, Zhijian Xu, Ning Wang, Ruyong Yao, Tao Qin, Hao Lin, and Lu Yue
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Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Pancreatic cancer is one of the most common malignant tumors of the digestive tract, and it has a poor prognosis. Traditional methods are not effective to accurately assess the prognosis of patients with pancreatic cancer. Immunotherapy is a new promising approach for the treatment of pancreatic cancer; however, some patients do not respond well to immunotherapy, which may be related to tumor microenvironment regulation. In this study, we use gene expression database to mine important immune genes and establish a prognostic prediction model for pancreatic cancer patients. We hope to provide a feasible method to evaluate the prognosis of pancreatic cancer and provide valuable targets for pancreatic cancer immunotherapy. Results We used univariate COX proportional hazard regression analysis, the least absolute shrinkage and selection operator, and multivariate COX regression analysis to screen 8 genes related to prognosis from the 314 immune-related genes, and used them to construct a new clinical prediction model in the TCGA pancreatic cancer cohort. Subsequently, we evaluated the prognostic value of the model. The Kaplan–Meier cumulative curve showed that patients with low risk scores survived significantly longer than patients with high risk scores. The area under the ROC curve (AUC value) of the risk score was 0.755. The univariate COX analysis showed that the risk score was significantly related to overall survival (HR 1.406, 95% CI 1.237–1.598, P
- Published
- 2021
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7. Occupational exposure to carbon black nanoparticles increases inflammatory vascular disease risk: an implication of an ex vivo biosensor assay
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Jinglong Tang, Wenting Cheng, Jinling Gao, Yanting Li, Ruyong Yao, Nathaniel Rothman, Qing Lan, Matthew J. Campen, Yuxin Zheng, and Shuguang Leng
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Carbon black nanoparticles ,Biosensor ,Endothelial cell activation ,Mediation effect ,Toxicology. Poisons ,RA1190-1270 ,Industrial hygiene. Industrial welfare ,HD7260-7780.8 - Abstract
Abstract Background Among manufactured or engineered nanoparticles, carbon black (CB) has largest production worldwide and is also an occupational respiratory hazard commonly seen in rubber industry. Few studies have assessed the risk for cardiovascular disease in carbon black exposed populations. An endothelial biosensor assay was used to quantify the capacity of sera from 82 carbon black packers (CBP) and 106 non-CBPs to induce endothelial cell activation ex vivo. The mediation effect of circulatory proinflammatory factors on the association between carbon black exposure and endothelial cell activation was assessed and further validated using in vitro intervention experiments. Results The average elemental carbon level inside carbon black bagging facilities was 657.0 μg/m3, which was 164-fold higher than that seen in reference areas (4.0 μg/m3). A global index was extracted from mRNA expression of seven candidate biosensor genes using principal component analysis and used to quantify the magnitude of endothelial cell activation. This global index was found to be significantly altered in CBPs compared to non-CBPs (P
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- 2020
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8. Semiconductor laser irradiation improves root canal sealing during routine root canal therapy.
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Dandan Su, Xingxue Hu, Dashan Wang, Ting Cui, Ruyong Yao, and Huibin Sun
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Medicine ,Science - Abstract
To evaluate the effect of semiconductor laser irradiation on root canal sealing after routine root canal therapy (RCT).Sixty freshly extracted single-rooted human teeth were randomly divided into six groups (n = 10). The anatomic crowns were sectioned at the cementoenamel junction and the remaining roots were prepared endodontically with conventional RCT methods. Groups A and B were irradiated with semiconductor laser at 1W for 20 seconds; Groups C and D were ultrasonically rinsed for 60 seconds as positive control groups; Groups E and F without treatment of root canal prior to RCT as negative control groups. Root canal sealing of Groups A, C and E were evaluated by measurements of apical microleakage. The teeth from Groups B, D and F were sectioned, and the micro-structures were examined with scanning electron microscopy (SEM). One way ANOVA and LSD-t test were used for statistical analysis (α = .05).The apical sealing of both the laser irradiated group and the ultrasonic irrigated group were significantly different from the control group (p0.5). SEM observation showed that most of the dentinal tubules in the laser irradiation group melted, narrowed or closed, while most of the dentinal tubules in the ultrasonic irrigation group were filled with tooth paste.The application of semiconductor laser prior to root canal obturation increases the apical sealing of the roots treated.
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- 2017
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9. Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property
- Author
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Yanan Wang, Xuehong Chen, Xiaolin Bi, Yining Ren, Qi Han, Yu Zhou, Yantao Han, Ruyong Yao, and Shangyong Li
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Alginate lyase ,Thermo-tolerant ,pH-stability ,Endo-manner ,Vibrio sp. SY01 ,Biology (General) ,QH301-705.5 - Abstract
Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene, aly08, was cloned from the marine bacterium Vibrio sp. SY01 and expressed in Escherichia coli. The purified alginate lyase Aly08, with a molecular weight of 35 kDa, showed a specific activity of 841 U/mg at its optimal pH (pH 8.35) and temperature (45 °C). Aly08 showed good pH-stability, as it remained more than 80% of its initial activity in a wide pH range (4.0−10.0). Aly08 was also a thermo-tolerant enzyme that recovered 70.8% of its initial activity following heat shock treatment for 5 min. This study also demonstrated that Aly08 is a polyG-preferred enzyme. Furthermore, Aly08 degraded alginates into disaccharides and trisaccharides in an endo-manner. Its thermo-tolerance and pH-stable properties make Aly08 a good candidate for further applications.
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- 2019
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10. Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
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Yu Zhou, Xuehong Chen, Xiao Li, Yantao Han, Yanan Wang, Ruyong Yao, and Shangyong Li
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chitosanase ,cold-adaptation ,thermo-tolerance ,chitooligosaccharide ,Organic chemistry ,QD241-441 - Abstract
Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides.
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- 2019
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11. Polymorphisms of ERCC1, XPD, XRCC1 and XPG Predict Clinical Outcome in Advanced Gastric Cancer Patients Receiving Oxaliplatin-Based Chemotherapy in Chinese Population
- Author
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Jian JIANG, Jun LIANG, and Ruyong YAO
- Subjects
gastric cancer ,polymorphism ,oxaliplatin ,chemotherapy ,ERCC1 ,XPD ,XPG ,XRCC1. ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
OBJECTIVE To investigate whether polymorphisms in ERC1, XPD, XPG, XRCC1 genes are associated with clinical outcomes in advanced gastric cancer (AGC) patients treated with oxaliplatin-based chemotherapy. METHODS The genetic polymorphisms in ERCC1, XPD, XPG, XRCC1 were determined in 94 advanced gastric cancer patients treated with oxaliplatin-based chemotherapy, using TaqMan-MGB probes. The clinical response of 60 patients with stage IV disease, time to progression (TTP) and overall survival (OS) of 94 patients were evaluated.RESULTS The overall disease control rate (CR + PR + SD) of the 60 patients in stage IV was 70% (42/60). Patients with XRCC1 399 G/G, XPG 46 C/C genotypes showed enhanced response to the oxaliplatin-based chemotherapy compared to those with other genotypes (P < 0.05). The median OS and TTP of the patients were 5.5 months and 9.0 months, respectively. Among the 4 types of polymorphisms in the study, XRCC1 399 G/A + A/A, XPG 46 C/T + T/T genotypes were regarded to be associated with chemoresistance and poor survival ( P < 0.05). Combination analysis of the 2 polymorphisms using the Kaplan–Meier method revealed that the TTP and OS of the patients with a number of risk genotypes were signi fi cantly shortened ( P < 0.05). No significant association was found between the genotypes of the XPD codon 751, the ERCC1 codon 118 and the clinical outcome (P > 0.05). CONCLUSION Testing for XRCC1 399, XPG 46 polymorphisms may allow identification of the gastric cancer patients who will benefit from oxaliplatin-based chemotherapy. Specific polymorphisms may influence clinical outcomes of AGC patients. Selecting specific chemotherapy based on pretreatment genotyping represents an innovative strategy that warrants prospective studies.
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- 2009
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12. 7, 8-Dihydroxyflavone Protects an Endothelial Cell Line from H2O2 Damage.
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Bingxiang Wang, Qian Zhang, Ruyong Yao, Xiangping Liu, and Zhiqiang Qu
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Medicine ,Science - Abstract
7, 8-dihydroxyflavone (7, 8-DHF), a selective agonist for TrkB receptors, has been well studied for its neurotrophic functions. However, its roles outside the neural tissues have scarcely been studied as yet. In this study, we investigated the protecting roles of 7, 8-DHF in EA.hy926 cells, a human umbilic vein endothelial cell line which was exposed to hydrogen peroxide (H2O2). We found that 7, 8-DHF significantly protected the cells from being damaged by H2O2 through suppression of apoptosis, attenuation of inflammatory factor releasing and inhibition of reactive oxygen species generation. The potent biological effects of 7, 8-DHF were probably executed via its binding to TrkB receptors because the receptor specific antagonist ANA-12 significantly blocked its protecting effects. The protecting roles of 7, 8-DHF in EA.hy926 cells suggest that it will be a promising compound to be developed into a health product that definitely benefits endothelial functions and prevents cardiovascular diseases.
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- 2015
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13. Impact of NPM, TFF3 and TACC1 on the prognosis of patients with primary gastric cancer.
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Aiping Ding, Wenwen Zhao, Xiaoli Shi, Ruyong Yao, Fang Zhou, Lu Yue, Shihai Liu, and Wensheng Qiu
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Medicine ,Science - Abstract
NPM, TFF3 and TACC1 are molecular markers that play important roles in cell differentiation. Herein, we investigated their prognostic impact in patients with primary gastric cancer (GC) and determined whether they could be used as markers of more aggressive gastric carcinomas by detecting the extent of expression in human gastric carcinoma samples.Tumor tissue specimens from 142 GC patients were retrospectively retrieved and immunohistochemically evaluated. Correlations between NPM, TFF3 and TACC1 over-expression and clinicopathologic parameters, and their prognostic values were investigated with χ(2), Kaplan-Meier method, and Cox uni- and multivariate survival models. NPM, TFF3 and TACC1 expression was significantly higher in GC patients with poorly differentiated histologic type than that in patients with well differentiated histologic type. NPM expression was significantly higher in patients with hepatic metastasis or recurrence than that in patients without metastasis. TFF3 expression was significantly higher in patients with positive lymph node metastasis than that in patients with negative lymph node metastasis. Age, lymph node metastasis, and TFF3 and TACC1 over-expression were significantly correlated with low survival (P
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- 2013
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14. A dynamics association study of gut barrier and microbiota in hyperuricemia.
- Author
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Qiulan Lv, Jun Zhou, Changyao Wang, Xiaomin Yang, Yafei Han, Quan Zhou, Ruyong Yao, and Aihua Sui
- Subjects
GUT microbiome ,INTESTINAL barrier function ,HYPERURICEMIA ,URIC acid ,PATHOGENIC bacteria - Abstract
Introduction: The intricate interplay between gut microbiota and hyperuricemia remains a subject of growing interest. However, existing studies only provided snapshots of the gut microbiome at single time points, the temporal dynamics of gut microbiota alterations during hyperuricemia progression and the intricate interplay between the gut barrier and microbiota remain underexplored. Our investigation revealed compelling insights into the dynamic changes in both gut microbiota and intestinal barrier function throughout the course of hyperuricemia. Methods: The hyperuricemia mice (HY) were given intragastric administration of adenine and potassium oxalate. Gut microbiota was analyzed by 16S rRNA sequencing at 3, 7, 14, and 21 days after the start of the modeling process. Intestinal permeability as well as LPS, TNF-a, and IL-1ß levels were measured at 3, 7, 14, and 21 days. Results: We discovered that shifts in microbial community composition occur prior to the onset of hyperuricemia, key bacterial Bacteroidaceae, Bacteroides, and Blautia exhibited reduced levels, potentially fueling microbial dysbiosis as the disease progresses. During the course of hyperuricemia, the dynamic fluctuations in both uric acid levels and intestinal barrier function was accompanied with the depletion of key beneficial bacteria, including Prevotellaceae, Muribaculum, Parabacteroides, Akkermansia, and Bacteroides, and coincided with an increase in pathogenic bacteria such as Oscillibacter and Ruminiclostridium. This microbial community shift likely contributed to elevated lipopolysaccharide (LPS) and proinflammatory cytokine levels, ultimately promoting metabolic inflammation. The decline of Burkholderiaceae and Parasutterella was inversely related to uric acid levels, Conversely, key families Ruminococcaceae, Family_XIII, genera Anaeroplasma exhibited positive correlations with uric acid levels. Akkermansiaceae and Bacteroidaceae demonstrating negative correlations, while LPS-containing microbiota such as Desulfovibrio and Enterorhabdus exhibited positive correlations with intestinal permeability. Conclusion: In summary, this study offers a dynamic perspective on the complex interplay between gut microbiota, uric acid levels, and intestinal barrier function during hyperuricemia progression. Our study suggested that Ruminiclostridium, Bacteroides, Akkermansiaceae, Bilophila, Burkholderiaceae and Parasutterella were the key bacteria that play vital rols in the progress of hyperuricemia and compromised intestinal barrier, which provide a potential avenue for therapeutic interventions in hyperuricemia. [ABSTRACT FROM AUTHOR]
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- 2024
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15. NHE1 Mediates 5-Fu Resistance in Gastric Cancer via STAT3 Signaling Pathway
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Zhenni Sun, Luan Shufang, Ruyong Yao, Zan Shen, Tao Qin, Yasai Yao, Xiao-Mei Xu, and Lu Yue
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0301 basic medicine ,medicine.diagnostic_test ,biology ,Chemistry ,Transfection ,Stat3 Signaling Pathway ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,Western blot ,Cell culture ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer cell ,medicine ,biology.protein ,Cancer research ,Pharmacology (medical) ,STAT3 ,Transcription factor - Abstract
Background Several recent studies have addressed the role of Na+/H+ exchanger isoform 1 (NHE1) in tumor cell growth and apoptosis, including in gastric cancer. However, the role of NHE1 expression related to the 5-Fu resistance in gastric cancer has not been investigated. Methods The expression of NHE1 was examined by qPCR in the SGC7901/5-FU cell line and its parental cell line. pcDNA3.1-NHE1 and NHE1-siRNA were transfected to SGC7901/5-FU resistance cells and cell apoptosis was detected via TUNEL assay. The upstream activators in NHE1 mediated 5-Fu resistant gastric cancer cells were detected by Western blot and immunofluorescent. Results A significant increase of the expression of NHE1 was observed in SGC7901 5-FU resistance cells compared to the GES-1 and SGC7901 cell line. NHE1 can suppress the cell apoptosis of SGC7901 5-FU resistance cells and involved in cell cycle. Also, the migration and invasion of SGC7901 5-FU resistance cells were promoted by NHE1. NHE1 also increases the intracellular pH. The results of Western blot analysis showed that NHE1 overexpression induced an increase in the expression of phosphorylated activator transcription factor 3 (pSTAT3). The more obvious phosphorylated level was shown in the phosphorylated STAT3 at pSTAT3tyr705. Further investigations revealed that the constitutive activation of STAT3 may be induced by JAK1 and JAK2, and thus effect the 5-FU resistance by regulating NHE1. Discussion In summary, our findings provided evidence that NHE1 contributed to 5-Fu resistance in gastric cancer cells by regulating the JAK/STAT3 pathway. Therefore, NHE1 can be a useful marker for predicting and monitoring 5-Fu resistance.
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- 2020
16. Prognostic value of eight immune gene signatures in pancreatic cancer patients
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Hao Lin, Tao Qin, Ning Wang, Wenting Wang, Ruyong Yao, Zhijian Xu, and Lu Yue
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Oncology ,lcsh:Internal medicine ,medicine.medical_specialty ,Multivariate analysis ,lcsh:QH426-470 ,medicine.medical_treatment ,Internal medicine ,Pancreatic cancer ,Biomarkers, Tumor ,Tumor Microenvironment ,Genetics ,medicine ,Humans ,lcsh:RC31-1245 ,Genetics (clinical) ,Tumor microenvironment ,Models, Statistical ,Framingham Risk Score ,Proportional hazards model ,business.industry ,Univariate ,Immunotherapy ,Prognosis ,medicine.disease ,Pancreatic Neoplasms ,lcsh:Genetics ,Cohort ,business ,Research Article - Abstract
Background Pancreatic cancer is one of the most common malignant tumors of the digestive tract, and it has a poor prognosis. Traditional methods are not effective to accurately assess the prognosis of patients with pancreatic cancer. Immunotherapy is a new promising approach for the treatment of pancreatic cancer; however, some patients do not respond well to immunotherapy, which may be related to tumor microenvironment regulation. In this study, we use gene expression database to mine important immune genes and establish a prognostic prediction model for pancreatic cancer patients. We hope to provide a feasible method to evaluate the prognosis of pancreatic cancer and provide valuable targets for pancreatic cancer immunotherapy. Results We used univariate COX proportional hazard regression analysis, the least absolute shrinkage and selection operator, and multivariate COX regression analysis to screen 8 genes related to prognosis from the 314 immune-related genes, and used them to construct a new clinical prediction model in the TCGA pancreatic cancer cohort. Subsequently, we evaluated the prognostic value of the model. The Kaplan–Meier cumulative curve showed that patients with low risk scores survived significantly longer than patients with high risk scores. The area under the ROC curve (AUC value) of the risk score was 0.755. The univariate COX analysis showed that the risk score was significantly related to overall survival (HR 1.406, 95% CI 1.237–1.598, P Conclusions Based on the TCGA-PAAD cohort, we identified immune-related markers with independent prognostic significance, validated, and analyzed their biological functions, to provide a feasible method for the prognosis of pancreatic cancer and provide potentially valuable targets for pancreatic cancer immunotherapy.
- Published
- 2021
17. Rapid and reliable distinguish of Morinda officinalis How by loop-mediated isothermal amplification (LAMP) assay
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Quan Zhou, Sui Aihua, Wang Shuhan, Hang Xu, Han Yafei, and Ruyong Yao
- Subjects
Chromatography ,biology ,Chemistry ,Loop-mediated isothermal amplification ,biology.organism_classification ,Morinda officinalis - Abstract
The medicinal plant Morinda officinalis How (MO), especially the root, has been frequently used in traditional medicines around the world as an herbal drug for treating variable human disorders and diseases. Various adulterations of MO were found for economic or production limitations. However, authentication of MO from its adulterants by LAMP has not yet been established. The present study introduces a commercially available nucleic acid amplification method, loop-mediated isothermal amplification (LAMP) assay for the distinguish of MO from its adulterants. In this method, we combined DNA barcodes technology to design 2 pairs independent LAMP primers, which based on the internal transcribed spacer 2 (ITS2) sequence of MO’s nuclear ribosomal DNA. Our results showed that the LAMP could amplify the samples as expected and successfully identify target MO, and the limit for DNA template preciseness was verified as 1 × 10− 1 pg/µl. All the visual or real-time turbidity detection was performed within 60 min at approximately 63 °C. The result showed that the LAMP assay and primers we designed have high accuracy and efficiency for the differentiation of MO and its adulterants. Our results illustrated that the proposed low-cost, fast and reliable LAMP assay without the need for expensive equipment or specialized techniques could be a good way for MO rapid authentication.
- Published
- 2020
18. Occupational exposure to carbon black nanoparticles increases inflammatory vascular disease risk: an implication of an ex vivo biosensor assay
- Author
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Matthew J. Campen, Nathaniel Rothman, Shuguang Leng, Jinglong Tang, Ruyong Yao, Qing Lan, Jinling Gao, Yuxin Zheng, Wenting Cheng, and Yanting Li
- Subjects
Health, Toxicology and Mutagenesis ,Interleukin-1beta ,lcsh:Industrial hygiene. Industrial welfare ,Vascular Cell Adhesion Molecule-1 ,Air Pollutants, Occupational ,Pharmacology ,Toxicology ,Proinflammatory cytokine ,03 medical and health sciences ,Soot ,lcsh:RA1190-1270 ,Occupational Exposure ,medicine ,Humans ,Interleukin 8 ,Vascular Diseases ,Respiratory system ,lcsh:Toxicology. Poisons ,030304 developmental biology ,Inflammation ,0303 health sciences ,Cell adhesion molecule ,Chemistry ,Vascular disease ,Tumor Necrosis Factor-alpha ,Research ,Endothelial cell activation ,030311 toxicology ,General Medicine ,Carbon black ,medicine.disease ,Endothelial stem cell ,Mediation effect ,Carbon black nanoparticles ,Cell Adhesion Molecules ,lcsh:HD7260-7780.8 ,Ex vivo ,Biosensor - Abstract
Background Among manufactured or engineered nanoparticles, carbon black (CB) has largest production worldwide and is also an occupational respiratory hazard commonly seen in rubber industry. Few studies have assessed the risk for cardiovascular disease in carbon black exposed populations. An endothelial biosensor assay was used to quantify the capacity of sera from 82 carbon black packers (CBP) and 106 non-CBPs to induce endothelial cell activation ex vivo. The mediation effect of circulatory proinflammatory factors on the association between carbon black exposure and endothelial cell activation was assessed and further validated using in vitro intervention experiments. Results The average elemental carbon level inside carbon black bagging facilities was 657.0 μg/m3, which was 164-fold higher than that seen in reference areas (4.0 μg/m3). A global index was extracted from mRNA expression of seven candidate biosensor genes using principal component analysis and used to quantify the magnitude of endothelial cell activation. This global index was found to be significantly altered in CBPs compared to non-CBPs (P P = 0.74). Individual gene analyses identified that de novo expression of key adhesion molecules (e.g., ICAM and VCAM) and chemotactic factors (e.g., CCL2, CCL5, and CXCL8) responsible for the recruitment of leukocytes was dramatically induced in CBPs with CXCL8 showing the highest fold of induction (relative quantification = 9.1, P Conclusions Inflammatory mediators in sera from CBPs may bridge carbon black exposure and endothelial cell activation response assessed ex vivo. CBPs may have elevated risk for cardiovascular diseases when comorbidity exists. Our study may serve as a benchmark for understanding health effects of engineered carbon based nanoparticles with environmental and occupational health relevance.
- Published
- 2020
19. Using a PCR instrument to hydrolyze polysaccharides for monosaccharide composition analyses
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Meng Zhang, Min Niu, Ruyong Yao, Xiangping Liu, Yiran Zhang, Lijuan Zhang, and Xuexiao Ma
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chemistry.chemical_classification ,Chromatography ,Polymers and Plastics ,Fucoidan ,Hydrolysis ,Organic Chemistry ,Monosaccharides ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Polysaccharide ,01 natural sciences ,High-performance liquid chromatography ,Polymerase Chain Reaction ,0104 chemical sciences ,Monosaccharide composition ,Chitosan ,chemistry.chemical_compound ,chemistry ,Polysaccharides ,Materials Chemistry ,Monosaccharide ,Acid hydrolysis ,0210 nano-technology - Abstract
Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides. To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min. Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments. The hydrolysis loss ratio for monosaccharide standards were also measured. Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis. We further confirmed the reliability of the assay by HPLC coupled MS analysis. In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.
- Published
- 2020
20. Additional file 1 of Occupational exposure to carbon black nanoparticles increases inflammatory vascular disease risk: an implication of an ex vivo biosensor assay
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Jinglong Tang, Wenting Cheng, Jinling Gao, Yanting Li, Ruyong Yao, Rothman, Nathaniel, Lan, Qing, Campen, Matthew J., Yuxin Zheng, and Leng, Shuguang
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Data_FILES - Abstract
Additional file 1.
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- 2020
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21. P53 prevent tumor invasion and metastasis by down-regulating IDO in lung cancer
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Wen Gao, Ruyong Yao, Dongfang Tang, Lu Yue, Yuqin Yang, Lin Zhou, and Liming Lu
- Subjects
p53 ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Pathology ,IDO signaling ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Internal medicine ,medicine ,metastasis ,Stage (cooking) ,Lung cancer ,Therapeutic strategy ,Clinical pathology ,business.industry ,medicine.disease ,respiratory tract diseases ,lung cancer ,030104 developmental biology ,Cardiothoracic surgery ,030220 oncology & carcinogenesis ,Non small cell ,business ,Research Paper - Abstract
// Dongfang Tang 1 , Lu Yue 2 , Ruyong Yao 3 , Lin Zhou 4 , Yuqin Yang 4 , Liming Lu 4 and Wen Gao 1 1 Department of Thoracic Surgery, Huadong Hospital Affiliated to FuDan University, Huadong, China 2 Department of Oncology of the Qingdao Municipal Hospital, Qingdao, China 3 Central Laboratory of the Affiliated Hospital of Medical College Qingdao University, Qingdao, China 4 Central Laboratory of Shanghai Chest Hospital Affiliated to Shanghai Jiaotong University, China Correspondence to: Wen Gao, email: gaowenchest@163.com Keywords: lung cancer, IDO signaling, p53, metastasis Received: February 10, 2017 Accepted: March 29, 2017 Published: April 25, 2017 ABSTRACT In present study, we are to clear demonstrate the genetic evidence of IDO signaling’s impact on invasion and metastasis in lung cancer. Here we examined IDO1 expression levels in non-small cell lung cancer (NSCLC) patients (64) tumor/normal pairs underwent RT-PCR and comprehensive histological, immunohistochemica and clinical analysis. The NSCLC cells stably expressing IDO1 was analyzed for migration and invasion assays and the regulatory mechanism in vitro and metastasis assays in vivo . As results, we reported that IDO1 expression increased by more than 3.2-fold in lung cancer compared with their corresponding non-tumor tissues, and the up-regulation of IDO1 is significantly correlated to TNM stage and lymph node-metastasis. The over-expression of IDO1 significantly encouraged the metastasis and invasion of lung cancer cells, and IDO1 could promote metastasis formation in vivo . Furthermore, we further found that p53 could attenuate IDO signaling in lung cancer cell migration partly. In conclusion, these results demonstrate that the IDO signaling’s impact on invasion and metastasis and the suppressive effect of p53 on IDO1 in lung cancer, present one novel therapeutic strategy for early metastatic lung cancer in clinical.
- Published
- 2017
22. Upregulated microRNA-429 inhibits the migration of HCC cells by targeting TRAF6 through the NF-κB pathway
- Author
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Wang Liping, Peng Wang, Huazheng Pan, Ruyong Yao, Jia Cao, Jun Liang, Xiangping Liu, Shihai Liu, Aihua Sui, and Zimin Liu
- Subjects
0301 basic medicine ,Cancer Research ,Carcinoma, Hepatocellular ,Cell ,Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,Cell Line, Tumor ,microRNA ,medicine ,Animals ,Humans ,3' Untranslated Regions ,neoplasms ,Cell Proliferation ,TNF Receptor-Associated Factor 6 ,Oncogene ,Cell growth ,Liver Neoplasms ,Intracellular Signaling Peptides and Proteins ,NF-kappa B ,Hep G2 Cells ,General Medicine ,Cell cycle ,digestive system diseases ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Ectopic expression ,Neoplasm Transplantation ,Signal Transduction - Abstract
Increasing evidence indicates that miR-429 is involved in tumor suppression in various human cancers. however, its role in hepatocellular carcinoma (HCC) remains unclear. In the present study, we found that miR-429 was significantly downregulated in HCC tissue samples and cell lines. Upregulation of miR-429 markedly suppressed proliferation and migration of HCC cells. Moreover, we identified TRAF6 as a direct target of miR-429. Downregulation of TRAF6 partially attenuated the oncogenic effect of anti‑miR-429 on HCC cells. Ectopic expression of miR-429 in HCC cells inhibited TCF-4 activity as well as nuclear accumulation of P65 and expression of the NF-κB targets c-Myc and phosphorylation of TAK1. In a nude xenograft model, miR-429 upregulation significantly decreased HCC growth. In conclusion, by targeting TRAF6, miR-429 is downregulated in HCC and inhibits HCC cell proliferation and motility. Our data suggest that miR-429 may serve as a potential anticancer target for the treatment of HCC.
- Published
- 2017
23. lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA‑101
- Author
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Zhenni Sun, Lihua Deng, Lu Yue, Wenjun Yu, Han Yafei, Ling Yang, and Ruyong Yao
- Subjects
Male ,Cancer Research ,Lung Neoplasms ,Cell ,Apoptosis ,Biology ,NSCLC ,medicine.disease_cause ,Biochemistry ,cell migration and invasion ,Cell Movement ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,microRNA ,Genetics ,medicine ,Humans ,competing endogenous RNA ,Molecular Biology ,Cell Proliferation ,A549 cell ,Oncogene ,Cell growth ,Cell Cycle ,Articles ,Transfection ,Middle Aged ,Cell cycle ,miR-101 ,Immunohistochemistry ,respiratory tract diseases ,MicroRNAs ,medicine.anatomical_structure ,Oncology ,Cancer research ,Molecular Medicine ,Female ,RNA, Long Noncoding ,lncRNA PTAR ,Carcinogenesis - Abstract
MicroRNA (miR)-101 copy loss is an early event in the development of human lung cancer, and it occurs in 29% of all lung cancer incidences. In addition, miR-101 expression in non-small cell lung cancer (NSCLC) is known to be downregulated. The aim of the present study was to explore the roles and mechanisms of the long non-coding (lnc)-RNA pro-transition associated RNA (PTAR) on NSCLC cell proliferation, migration and invasion in association with miR-101. Reverse transcription-quantitative PCR analysis was performed to detect the expression of lncRNA PTAR in 30 paired human NSCLC tissues and the corresponding para-tumor tissues. PTAR was amplified and cloned into the expression vector pCDNA3.1. Then, PTAR-overexpression plasmids or small interfering (si)-RNA-PTAR was transfected into A549 cells for 48 h, after which cell proliferation and the cell cycle distribution were evaluated. In addition, Transwell chamber and cell scratch-wound assays were conducted to analyze A549 cell migration and invasion. A luciferase activity assay was evaluated to determine the interaction between PTAR and miR-101. Furthermore, our results demonstrated that in human NSCLC tissues and cell lines, lncRNA PTAR expression was upregulated compared with normal lung tissues and cell lines, respectively. Additionally, PTAR transfection was observed to promote A549 cell proliferation, migration and invasion; opposing effects were observed with siRNA-PTAR transfection. The luciferase activity assay revealed that PTAR could act as a sponge to bind miR-101. Thus, miR-101 plays a role in NSCLC tumorigenesis and progression. In conclusion, lncRNA PTAR was proposed to promote NSCLC cell growth through sponging and inactivating miR-101, which may be a possible mechanism underlying miR-101 copy loss in human NSCLC.
- Published
- 2019
24. Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property
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Xuehong Chen, Yining Ren, Yantao Han, Ruyong Yao, Yu Zhou, Xiaolin Bi, Yanan Wang, Shangyong Li, and Qi Han
- Subjects
pH-stability ,Aquatic Organisms ,Polysaccharide-Lyases ,Pharmaceutical Science ,Initial activity ,medicine.disease_cause ,Article ,Alginate lyase ,03 medical and health sciences ,Drug Discovery ,Enzyme Stability ,medicine ,Escherichia coli ,Vibrio sp. SY01 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,lcsh:QH301-705.5 ,030304 developmental biology ,Vibrio ,chemistry.chemical_classification ,0303 health sciences ,biology ,030306 microbiology ,Chemistry ,Temperature ,Hydrogen-Ion Concentration ,Thermo-tolerant ,biology.organism_classification ,Recombinant Proteins ,Enzyme ,Biochemistry ,lcsh:Biology (General) ,Specific activity ,Endo-manner ,Bacteria - Abstract
Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene, aly08, was cloned from the marine bacterium Vibrio sp. SY01 and expressed in Escherichia coli. The purified alginate lyase Aly08, with a molecular weight of 35 kDa, showed a specific activity of 841 U/mg at its optimal pH (pH 8.35) and temperature (45 °, C). Aly08 showed good pH-stability, as it remained more than 80% of its initial activity in a wide pH range (4.0&ndash, 10.0). Aly08 was also a thermo-tolerant enzyme that recovered 70.8% of its initial activity following heat shock treatment for 5 min. This study also demonstrated that Aly08 is a polyG-preferred enzyme. Furthermore, Aly08 degraded alginates into disaccharides and trisaccharides in an endo-manner. Its thermo-tolerance and pH-stable properties make Aly08 a good candidate for further applications.
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- 2019
25. Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium
- Author
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Yu, Zhou, Xuehong, Chen, Xiao, Li, Yantao, Han, Yanan, Wang, Ruyong, Yao, and Shangyong, Li
- Subjects
Molecular Weight ,Chitosan ,Pseudoalteromonas ,Bacterial Proteins ,Glycoside Hydrolases ,chitooligosaccharide ,Temperature ,Oligosaccharides ,thermo-tolerance ,Chitin ,chitosanase ,cold-adaptation ,Article - Abstract
Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides.
- Published
- 2018
26. Effect of oxaliplatin combined with polyenephosphatidylcholine on the proliferation of human gastric cancer SGC-7901 cells
- Author
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Ruyong Yao, Jianbin Li, Tao Jiang, Xiguang Liu, Yuanyuan Zhao, Hongjun Zhang, and Hao Song
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,Cell ,Biology ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,polyenephosphatidylcholine ,Internal medicine ,medicine ,MTT assay ,Oncogene ,Cell growth ,gastric cancer ,oxaliplatin ,Cancer ,Articles ,Cell cycle ,medicine.disease ,medicine.anatomical_structure ,Apoptosis ,SGC-7901 cells ,030220 oncology & carcinogenesis ,Cancer research ,030211 gastroenterology & hepatology ,Carcinogenesis - Abstract
Oxaliplatin (L-OHP) is a platinum compound that is widely used to treat certain solid tumors, including gastric tumors. L-OHP is an effective anti-cancer treatment; however, its usage increases the probability of patients developing hepatic injury with inflammation, referred to as chemotherapy-associated steatohepatitis. The present study aimed to evaluate the outcome of L-OHP treatment combined with polyenephosphatidylcholine (PPC), a major component of essential phospholipids used to treat steatohepatitis, on SGC-7901 gastric cancer cell proliferation. This would help to determine whether combination therapy with L-OHP and PPC is clinically beneficial for patients with gastric cancer. The viability of SGC-7901 cells was verified by an MTT assay; flow cytometry was used to analyze the cell cycle and rates of cell apoptosis; oxidation-related indicators were measured by spectrophotometry, and the expression of cell cycle- and apoptosis-related proteins was determined by western blotting. The results demonstrated that L-OHP significantly inhibited SGC-7901 cell growth in a dose- and time-dependent manner (F=194.193, P
- Published
- 2016
27. Apoptosis-inducing effect of 6,7-dimethoxy-4'-hydroxy-8- formylflavon from Nicotiana tabacum L leaf in human hepatoma HepG2 cells via activation of mitochondriamediated apoptotic pathway
- Author
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Liang Jun, Liu Zimin, Ruyong Yao, Wen-Sheng Qiu, Qiu-Jie Zhang, Zhuang Yu, Hong-Xia Cui, and Shihai Liu
- Subjects
biology ,medicine.diagnostic_test ,Chemistry ,Cytochrome c ,Nicotiana tabacum ,virus diseases ,Pharmaceutical Science ,biology.organism_classification ,Molecular biology ,In vitro ,Flow cytometry ,Western blot ,In vivo ,Apoptosis ,6,7-Dimethoxy-4'-hydroxy-8-formylflavon, HepG2 cells, Hepatoma, Mitochondria, Apoptosis, Bax, Cytochrome C, Survivin ,Survivin ,biology.protein ,medicine ,Pharmacology (medical) - Abstract
Purpose : To study the anti-proliferative and apoptotic influences of 6,7-dimethoxy-4'-hydroxy-8- formylflavon (DHF) from the leaves of Nicotiana tabacum L. in human hepatoma HepG2 cells, and the underlying mechanisms. Methods : The anti-proliferative effect of DHF (10 - 50 μg/mL) on HepG2 cells was assessed by CCK-8 assay. The pro-apoptotic effect of DHF (10, 20 and 30 μg/mL) on HepG2 cells was investigated via flow cytometry, while the mechanisms involved were studied using western blot. Xenograft assay was employed for determination of the in vivo effect of DHF (40 mg/kg/day) on HepG2 cell-induced tumor. Results : The proliferation of HepG2 cells was inhibited by DHF (IC 50 = 25.87 μg/mL) due to apoptosis. In addition, xenograft assay revealed that HepG2 cell-induced tumor growth was significantly suppressed by DHF (p < 0.05 or 0.01) without any effects on mice body weights. The expressions of Survivin and Bcl-2 proteins were significantly decreased, while those of Bax, c-caspase-9, and ccaspase- 3 proteins were significantly increased by DHF (p < 0.05 or 0.01), leading to increase in cytoplasmic levels of Smac and cytochrome c proteins. Conclusion : The underlying mechanism DHF-mediated apoptotic changes in HepG2 cells in vitro and in vivo involves induction of the mitochondrial pathway of apoptosis. Thus, DHF is a good drug candidate for the development of an effective therapy for liver cancer. Keywords : 6,7-Dimethoxy-4'-hydroxy-8-formylflavon, HepG2 cells, Hepatoma, Mitochondria, Apoptosis, Bax, Cytochrome C, Survivin
- Published
- 2018
28. Trichostatin A reverses the chemoresistance of lung cancer with high IGFBP2 expression through enhancing autophagy
- Author
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Yifeng Sun, Dandan Zhao, Lin Zhou, Dongfang Tang, Wen Gao, Yang Yang, Ruyong Yao, Liming Lu, and Yun Wu
- Subjects
Male ,0301 basic medicine ,Lung Neoplasms ,lcsh:Medicine ,Adenocarcinoma ,Hydroxamic Acids ,medicine.disease_cause ,Article ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Autophagy ,Tumor Cells, Cultured ,medicine ,Carcinoma ,Humans ,Neoplasm ,lcsh:Science ,Lung cancer ,Survival rate ,Retrospective Studies ,Multidisciplinary ,business.industry ,lcsh:R ,Middle Aged ,Prognosis ,medicine.disease ,Histone Deacetylase Inhibitors ,Survival Rate ,Insulin-Like Growth Factor Binding Protein 2 ,030104 developmental biology ,Trichostatin A ,Drug Resistance, Neoplasm ,Case-Control Studies ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Cancer research ,Female ,lcsh:Q ,business ,Carcinogenesis ,medicine.drug - Abstract
Insulin-like growth factor (IGF) signaling plays an important role in tumorigenesis and metastasis. Here, we analyzed insulin-like growth factor (IGF) binding protein-2 (IGFBP2) expression in 81 lung cancer patients and 36 controls consisting of healthy and benign pulmonary lesion participants for comparison, then validated the IGFBP2 expression in additional 84 lung cancer patients, and evaluated the prognostic and chemoresistant significance of IGFBP2 in two cohorts respectively. Next we detected the reversal effect of trichostatin A (TSA) on chemoresistance in cell lines with high IGFBP2 expression. As a result, the mean expression of IGFBP2 in lung cancer patients was significantly higher than that in controls and increased with lung cancer progressed to advanced stage. In addition, high IGFBP2 expression was independently predictive for chemoresistance; over-expressed IGFBP2 enhances cell activity and TSA can reverse the chemoresistance induced by high IGFBP2 expression through enhancing autophagy. Furthermore, multivariate analysis showed that lung cancer patients whose blood IGFBP2 was higher had a poor survival outcome, with a hazard ratio of 8.22 (95%CI 1.78–37.92, P = 0.007) after adjustment for stage, histopathology, EGFR mutation, age, smoking and surgery.
- Published
- 2018
29. <scp>CUG</scp> ‐binding protein 1 ( <scp>CUGBP</scp> 1) expression and prognosis of brain metastases from non‐small cell lung cancer
- Author
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Xiaofei Wang, Ruyong Yao, Yongjie Wang, Yandong Zhao, Y Luo, Jinpeng Zhao, Wenjie Jiao, Liangdong Zhang, and M. Wang
- Subjects
non‐small cell lung cancer ,0301 basic medicine ,Pulmonary and Respiratory Medicine ,Oncology ,medicine.medical_specialty ,CUGBP1 ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,law ,Internal medicine ,medicine ,Lung cancer ,Ki‐67 ,Polymerase chain reaction ,Messenger RNA ,biology ,Proportional hazards model ,business.industry ,Brain metastases ,Original Articles ,General Medicine ,medicine.disease ,respiratory tract diseases ,030104 developmental biology ,030220 oncology & carcinogenesis ,Ki-67 ,biology.protein ,Immunohistochemistry ,Original Article ,Non small cell ,business - Abstract
Background The brain is a frequent site of metastases from non-small cell lung cancer (NSCLC). The purpose of this study was to detect the expression of CUG-binding protein 1 (CUGBP1) messenger ribonucleic acid (mRNA) and Ki-67 in metastasized brain tissue from NSCLC and determine the relationship between CUGBP1 and brain metastases. Methods The expression of CUGBP1 mRNA and Ki-67 in metastasized brain tissue from NSCLC was investigated by semiquantitative polymerase chain reaction and immunohistochemistry, respectively. The expression of CUGBP1 and Ki-67 in metastasized brain tissue from NSCLC was related to clinical characteristics, as assessed using the chi-square test. The prognostic significance was assessed by univariate and multivariate analyses using the Cox hazard model. Results The expression of CUGBP1 mRNA and Ki-67 was overexpressed in metastasized brain tissue from NSCLC and was correlated with differentiation. In addition, by both univariate and multivariate survival analyses, CUGBP1 expression, Ki-67 expression, and age were noted to be independent indicators of a shorter postsurgical survival. Conclusion The expression of CUGBP1 is an important factor in the development of brain metastases from NSCLC.
- Published
- 2015
30. miR-141 confers docetaxel chemoresistance of breast cancer cells via regulation of EIF4E expression
- Author
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Wensheng Qiu, Yasai Yao, Li‑Kun Zhuang, Ruyong Yao, Libin Sun, Qian Zhang, Fei Zhou, and Lu Yue
- Subjects
Nucleocytoplasmic Transport Proteins ,Cancer Research ,Cell ,Apoptosis ,Breast Neoplasms ,Docetaxel ,Drug resistance ,medicine.disease_cause ,Cell Line, Tumor ,medicine ,Humans ,RNA, Messenger ,Regulation of gene expression ,Oncogene ,Chemistry ,Cancer ,General Medicine ,medicine.disease ,Molecular medicine ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,medicine.anatomical_structure ,Oncology ,Drug Resistance, Neoplasm ,MCF-7 Cells ,Cancer research ,Female ,Taxoids ,Carcinogenesis ,medicine.drug - Abstract
Resistance to docetaxel, a chemotherapy drug for breast cancer (BC) treatment, occurs in ~50% of patients, and the underlying molecular mechanisms of drug resistance are not fully understood. Gene regulation through miR-141 has been proven to play an important role in cancer drug resistance. The present study investigated the role of miR-141 expression in BC cells of acquired docetaxel resistance. Inhibition of miR-141 enhanced the response to docetaxel in docetaxel-resistant cells (MCF-7/DTX and MDA-MB-231/DTX, respectively), whereas overexpression of miR-141 confered resistance in docetaxel-sensitive cells (MCF-7 and MDA-MB-231, respectively). By directly targeting the eukaryotic translation initiation factor 4E (EIF4E) mRNA, miR-141 acts on genes that are necessary for drug induced apoptosis rendering the cells drug resistant. Modulation of miR-141 expression was correlated with EIF4E expression changes and a direct interaction of miR-141 with EIF4E was shown by a luciferase assay. Thus, the present study is the first to show an increased expression of miR-141 in an acquired model of docetaxel resistance in BC. This serves as a mechanism of acquired docetaxel resistance in BC cells, possibly through direct interactions with EIF4E, therefore presenting a potential therapeutic target for the treatment of docetaxel resistant BC.
- Published
- 2015
31. MiR‐200b expression in breast cancer: a prognostic marker and act on cell proliferation and apoptosis by targeting Sp1
- Author
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Shihai Liu, Zan Shen, Xingang Wang, Wensheng Qiu, Lu Yue, Ruyong Yao, Yong Li, Jian Hu, Yasai Yao, and Hui Cong
- Subjects
Sp1 Transcription Factor ,Blotting, Western ,MiR-200b ,Apoptosis ,Breast Neoplasms ,Kaplan-Meier Estimate ,Biology ,Sp1 ,breast cancer ,Western blot ,Cell Line, Tumor ,microRNA ,Biomarkers, Tumor ,medicine ,cell growth ,Humans ,3' Untranslated Regions ,Cell Proliferation ,Neoplasm Staging ,Gene knockdown ,medicine.diagnostic_test ,Reverse Transcriptase Polymerase Chain Reaction ,Cell growth ,Cancer ,Original Articles ,Cell Biology ,Middle Aged ,Cell cycle ,Prognosis ,medicine.disease ,Molecular biology ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cell culture ,MCF-7 Cells ,Molecular Medicine ,Female ,RNA Interference - Abstract
MicroRNAs (miRNAs) have been identified as important post-transcriptional regulators involved in various biological and pathological processes of cells. In the present study, we investigated the roles and mechanisms of miR-200b in human breast cancer (BC). MiR-200b expression was carried out by qRT-PCR in human BC cell lines and clinical samples and the prognostic potential of miR-200b expression was further evaluated. In vitro, effects of miR-200b on BC cell proliferation, apoptosis and cell cycle distribution were tested by CCK-8 kit, flow cytometric analysis respectively. Luciferase assay and Western blot analysis were performed to validate the potential targets of miR-200b after the preliminary screening by employing open access software. We found that miR-200b was significantly down-regulated in both BC tissues and cell lines. The low expression of miR-200b was correlated with late TNM stage, negative oestrogen receptor and positive HER-2 status. Multivariate analysis showed that miR-200b expression was an independent prognostic predictor for BC patients. Integrated analysis identified Sp1 as a direct and functional target of miR-200b. Knockdown of Sp1 inhibited cell proliferation, induce apoptosis and act on cell cycle resembling that of miR-200b high expression. Our data demonstrates that miR-200b has potential to serve as prognostic biomarker and tumour suppressor for BC patients. As a direct and functional target of miR-200b, Sp1 and miR-200b both could be an exciting target for BC treatment strategy.
- Published
- 2015
32. MOR1 Expression in Gastric Cancer: A Biomarker Associated With Poor Outcome
- Author
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Lu Yue, Weiwei Qi, M M Wensheng Qiu, Fei Zhou, Ruyong Yao, M M Jing Lv, Li-kun Zhuang, and M M Ya-sai Yao
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,General Neuroscience ,Cancer ,General Medicine ,Lymph node metastasis ,medicine.disease ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Depth of invasion ,Tumor progression ,Internal medicine ,medicine ,Overall survival ,Biomarker (medicine) ,Immunohistochemistry ,General Pharmacology, Toxicology and Pharmaceutics ,business - Abstract
Background At present, the expression of MOR1 and its function in gastric cancer remains unclear with evidence suggesting that it is to be involved in tumor progression and metastasis. The study was to assess the clinicopathologic relevance and prognostic value of MOR1 expression in gastric cancer. Methods Real-time quantitative RT-PCR and immunohistochemical staining were used to detect MOR1 expression in primary gastric cancerous surgical specimens and adjacent nontumorous tissues. Results High MOR1 expression was detected in cancerous tumor compared with their adjacent nontumorous tissues. In addition, the chi-square test revealed that high MOR1 expression was significantly correlated with depth of invasion (p = 0.006), lymph node metastasis (p = 0.001), distant metastasis (p = 0.017), and TNM staging (p = 0.027). Moreover, Kaplan–Meier analysis revealed a significant association between MOR1 expression and overall survival. High expression of MOR1 was identified as an independent and significant predictor gene of reduced postoperative survival. Conclusion We conclude that MOR1 expression may be a useful biomarker for better prediction of the clinical outcome and management of gastric cancer patients.
- Published
- 2014
33. The tumor suppressor miR-124 inhibits cell proliferation by targeting STAT3 and functions as a prognostic marker for postoperative NSCLC patients
- Author
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Caihong Gao, Xiumei Li, Shihai Liu, Ruyong Yao, Yong Li, Xin Hou, Zhuang Yu, and Lianhua Cui
- Subjects
Adult ,Male ,STAT3 Transcription Factor ,Oncology ,Cancer Research ,medicine.medical_specialty ,Cell ,Apoptosis ,Biology ,Disease-Free Survival ,Carcinoma, Non-Small-Cell Lung ,Internal medicine ,Carcinoma ,medicine ,Humans ,Lung cancer ,Aged ,Cell Proliferation ,Neoplasm Staging ,Oncogene ,Cancer ,Middle Aged ,Cell cycle ,Prognosis ,medicine.disease ,Molecular medicine ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,medicine.anatomical_structure ,Cancer research ,Biomarker (medicine) ,Female ,Lymph Nodes - Abstract
The aim of the present study was to investigate the role of miR-124 in lung cancer and identify the potential predictive value of miR-124 in postoperative non-small cell lung cancer (NSCLC) patients. We detected miR-124 expression in A549, NCL-H460 and normal lung epithelial BEAS-2E cells and showed a significantly lower expression level of miR-124 in NSCLC cells than in BEAS-2E cells. Upregulation of miR-124 expression levels in both A549 and NCL-H460 cells by transfection with miR-124 mimics suppressed cell proliferation and induced apoptosis. Further investigation revealed that miR-124 bound directly to the 3' UTR region of STAT3, thereby inhibiting STAT3 expression. In addition, miR-124 levels detected in NSCLC tissues were lower than those in adjacent normal lung tissues, while the opposite was observed for STAT3. In NSCLC, the expression levels of miR-124 and STAT3 correlated significantly with the tumor node metastases (TNM) stage, differentiation grade and lymph node metastasis, while the levels of these molecules did not differ significantly by gender, age, location, smoking index, pleural invasion or pathological type. The expression level of miR-124 was significantly associated with disease-free survival (DFS) in both positive and negative lymph node groups. Furthermore, patients with low miR-124 or high STAT3 expression generally received a worse prognosis in terms of both overall survival (OS) and DFS. In conclusion, our findings suggest that miR-124 functions as a tumor suppressor by targeting STAT3, and that miR-124 may potentially serve as a useful biomarker for the prognosis of NSCLC patients.
- Published
- 2014
34. The miR-3127-5p/p-STAT3 axis up-regulates PD-L1 inducing chemoresistance in non-small-cell lung cancer
- Author
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Ruyong Yao, Yifeng Sun, Dongfang Tang, Liming Lu, Yun Wu, Dandan Zhao, Wen Gao, and Lin Zhou
- Subjects
0301 basic medicine ,medicine.drug_class ,Monoclonal antibody ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,PD-L1 ,medicine ,STAT3 ,Lung cancer ,JAK/STAT3 ,biology ,medicine.diagnostic_test ,Chemistry ,Autophagy ,immune escape ,Cell Biology ,Original Articles ,medicine.disease ,microRNA‐3127‐5p ,lung cancer ,030104 developmental biology ,Real-time polymerase chain reaction ,PD‐L1 ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Molecular Medicine ,Phosphorylation ,Original Article - Abstract
It is less known about miRNA3127‐5p induced up‐regulation of PD‐L1, immune escape and drug resistance caused by increased PD‐L1 in lung cancer. In this study, lentivirus was transduced into lung cancer cells, and quantitative PCR and Western blot were used to detect the expression of PD‐L1. Then immunofluorescence assay was applied to detect autophagy, finally we explored the relationship between PD‐L1 expressions and chemoresistance in patients. As a result, we found that microRNA‐3127‐5p promotes pSTAT3 to induce the expression of PD‐L1; microRNA‐3127‐5p promotes STAT3 phosphorylation through suppressing autophagy, and autophagy could retaine pSTAT3 into the nucleus in miRNA‐3127‐5p knocked cells, and immune escape induced by elevated level of PD‐L1 results in chemoresistance of lung cancer. In conclusion, microRNA‐3127‐5p induces PD‐L1 elevation through regulating pSTAT3 expression. We also demonstrate that immune escape induced by PD‐L1 can be dismissed by corresponding monoclonal antibody.
- Published
- 2017
35. Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells
- Author
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Lu Yue, Yan Xu, Han Yafei, Ruyong Yao, Jisheng Zhang, Jingfang Zhang, and Zhenni Sun
- Subjects
0301 basic medicine ,Cancer Research ,Gene knockdown ,Oncogene ,Cancer ,Articles ,Cell cycle ,Biology ,medicine.disease ,medicine.disease_cause ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,medicine ,Viability assay ,Carcinogenesis ,A431 cells - Abstract
Rnf2 is a fundamental component of the polycomb repressive complex 1and acts as the really interesting new gene finger E3 ligase, which is responsible for histone 2A modification. Previous studies have shown that the ring finger protein 2 (Rnf2) is overexpressed in various types of tumor and has a close association with tumor development. However, few studies have been carried out into the expression and biological function of Rnf2 in gastric cancer cells. The present study measured the expression of Rnf2 in gastric cancer cells and normal epithelial gastric cells. The results demonstrate that Rnf2 is upregulated in gastric cancer cells. In addition, the knockdown of Rnf2 inhibited the cell viability and induced increased G1 phase followed by a substantial reduction of the G2/M phase. The expression levels of p21 and p27 were also significantly elevated by the knockdown of Rnf2. These results provide evidence of the oncogenic function of Rnf2 in gastric cancer, possibly through an inhibition of cellular proliferation and a delay of the G2/M phase. Therefore, Rnf2 may be a novel target for the prognosis and therapy of gastric cancer.
- Published
- 2017
36. Semiconductor laser irradiation improves root canal sealing during routine root canal therapy
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Xingxue Hu, Dandan Su, Dashan Wang, Huibin Sun, Ruyong Yao, and Ting Cui
- Subjects
Teeth ,Sanitization ,Root canal ,Positive control ,Dentistry ,lcsh:Medicine ,Transportation ,law.invention ,030207 dermatology & venereal diseases ,0302 clinical medicine ,law ,Medicine and Health Sciences ,Dentin ,Ultrasonics ,Electron Microscopy ,Public and Occupational Health ,Coloring Agents ,lcsh:Science ,Microscopy ,Multidisciplinary ,Hypochlorites ,Transportation Infrastructure ,Root Canal Therapy ,Chemistry ,Cementoenamel junction ,Infectious Diseases ,medicine.anatomical_structure ,Dentinal Tubule ,Optical Equipment ,Physical Sciences ,Engineering and Technology ,Scanning Electron Microscopy ,Lasers, Semiconductor ,Anatomy ,Research Article ,Materials science ,Infectious Disease Control ,Materials by Structure ,Materials Science ,Equipment ,Research and Analysis Methods ,Civil Engineering ,Root Canal Filling Materials ,03 medical and health sciences ,medicine ,Humans ,Irradiation ,Root Canal Obturation ,business.industry ,Lasers ,lcsh:R ,Chemical Compounds ,Biology and Life Sciences ,030206 dentistry ,Laser ,Health Care ,Disinfection ,Semiconductors ,Jaw ,Canals ,Salts ,lcsh:Q ,Preventive Medicine ,Dental Pulp Cavity ,Gutta-Percha ,business ,Digestive System ,Head - Abstract
Objective To evaluate the effect of semiconductor laser irradiation on root canal sealing after routine root canal therapy (RCT). Methods Sixty freshly extracted single-rooted human teeth were randomly divided into six groups (n = 10). The anatomic crowns were sectioned at the cementoenamel junction and the remaining roots were prepared endodontically with conventional RCT methods. Groups A and B were irradiated with semiconductor laser at 1W for 20 seconds; Groups C and D were ultrasonically rinsed for 60 seconds as positive control groups; Groups E and F without treatment of root canal prior to RCT as negative control groups. Root canal sealing of Groups A, C and E were evaluated by measurements of apical microleakage. The teeth from Groups B, D and F were sectioned, and the micro-structures were examined with scanning electron microscopy (SEM). One way ANOVA and LSD-t test were used for statistical analysis (α = .05). Results The apical sealing of both the laser irradiated group and the ultrasonic irrigated group were significantly different from the control group (p0.5). SEM observation showed that most of the dentinal tubules in the laser irradiation group melted, narrowed or closed, while most of the dentinal tubules in the ultrasonic irrigation group were filled with tooth paste. Conclusion The application of semiconductor laser prior to root canal obturation increases the apical sealing of the roots treated.
- Published
- 2017
37. <scp>FOXM</scp> 1 mediates resistance to docetaxel in gastric cancer via up‐regulating Stathmin
- Author
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Shihai Liu, Ruyong Yao, Yasai Yao, Jun Liang, Lu Yue, Wensheng Qiu, Weiwei Qi, and Xiaoxiao Li
- Subjects
Kinesins ,Apoptosis ,Stathmin ,Docetaxel ,Biology ,urologic and male genital diseases ,Microtubules ,Downregulation and upregulation ,Gastrectomy ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Humans ,Gene silencing ,Gene Silencing ,docetaxel resistance ,Promoter Regions, Genetic ,neoplasms ,Proportional Hazards Models ,gastric cancer ,Forkhead Box Protein M1 ,FOXM1 ,Cancer ,Forkhead Transcription Factors ,Original Articles ,Cell Biology ,Prognosis ,microtubule dynamics ,medicine.disease ,Thiostrepton ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Phenotype ,Drug Resistance, Neoplasm ,Multivariate Analysis ,Cancer cell ,biology.protein ,Cancer research ,Molecular Medicine ,Taxoids ,therapeutics ,MCAK ,medicine.drug - Abstract
Docetaxel is commonly used as an effective chemotherapeutic drug for gastric cancer patients recently. With the increasing emergence of docetaxel resistance nowadays, identification of suitable biomarkers for predicting chemosensitivity to docetaxel may be a key role for improving therapeutic effects for gastric cancer patients. In this study, we investigated the correlation between the expression of transcription factor forkhead box protein M1 (FOXM1) and chemotherapy response to docetaxel in gastric cancer, the possible mechanism for which was further explored. As a result, FOXM1 overexpression was shown to mediate resistance to docetaxel in gastric cancers. It altered microtubule dynamics to protect tumour cells from docetaxel-induced apoptosis. Mechanistic investigations revealed that tubulin-destabilizing protein Stathmin, which mediated docetaxel resistance in FOXM1-silenced gastric cancer cells, is a direct down-stream target of FOXM1, whereas another microtubule dynamics protein mitotic centromere–associated kinesin (MCAK), shown to be related to docetaxel resistance in gastric cancer cells, is not associated with FOXM1 expression significantly. These results were further provided by immunohistochemical analysis, indicating that FOXM1 and Stathmin expression levels were correlated in 103 post-operational gastric cancer specimens. Moreover, when we attenuated FOXM1 expression with FOXM1 inhibitor thiostrepton, docetaxel resistance in gastric cancers was found to be reversed, simultaneously with the down-regulation of FOXM1 and Stathmin. Therefore, FOXM1 can be a useful marker for predicting and monitoring docetaxel response. Through the inhibition of FOXM1, docetaxel resistance can be reversed, and thus FOXM1 could be a new therapeutic target in docetaxel-resistant gastric cancer.
- Published
- 2014
38. The Ca2+-activated Cl− channel, ANO1 (TMEM16A), is a double-edged sword in cell proliferation and tumorigenesis
- Author
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Criss Hartzell, Ruyong Yao, Xiangping Liu, Weicheng Yao, Zhiqiang Qu, and Kuai Yu
- Subjects
MAPK/ERK pathway ,Cancer Research ,Carcinogenesis ,Reviews ,ANO1 ,Biology ,medicine.disease_cause ,Malignant transformation ,Ca2+-activated Cl− channel ,Chloride Channels ,Neoplasms ,medicine ,cancer ,Humans ,Radiology, Nuclear Medicine and imaging ,Calcium Signaling ,Anoctamin-1 ,Calcium signaling ,Mitogen-Activated Protein Kinase Kinases ,Cell growth ,Cancer ,medicine.disease ,Cell biology ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,tumorigenesis ,cell proliferation ,Oncology ,biology.protein ,Calcium ,Signal transduction ,Signal Transduction - Abstract
Since anoctamin 1 ANO1 (TMEM16A) was found to be a molecular component of Ca(2+) -activated Cl(-) channels, its role in tumorigenesis has gained attention at a fast pace. ANO1 overexpression frequently occurs in the cancer tissues along with 11q13 chromosome amplification. Poor prognosis of many types of cancers has been closely correlated with ANO1 gene amplification and protein overexpression. ANO1 is now considered an excellent biomarker for certain cancers. Recent research suggests that it is the channel function of ANO1 that is involved in the tumorigenesis. However, how the overexpression of the functional ANO1 causes malignant transformation of tissues via signaling pathways, for example, MAPK remains to be investigated. Clarification of the reasons in future will avail to make ANO1 as a target for cancer treatment.
- Published
- 2014
39. Spermine attenuates the preconditioning of diazoxide against transient focal cerebral ischemia in rats
- Author
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Yu Li, Lin Zhang, Ruyong Yao, Shilei Wang, Peng Wang, Yunliang Guo, Shuhong Li, and Huan-li Dong
- Subjects
Male ,Potassium Channels ,Adult male ,Ischemia ,chemistry.chemical_element ,Spermine ,Apoptosis ,Calcium ,Mitochondrion ,Pharmacology ,Severity of Illness Index ,Random Allocation ,chemistry.chemical_compound ,medicine ,Diazoxide ,Animals ,Rats, Wistar ,bcl-2-Associated X Protein ,Neurons ,business.industry ,Brain ,Cytochromes c ,Infarction, Middle Cerebral Artery ,General Medicine ,medicine.disease ,Potassium channel ,Neuroprotective Agents ,Neurology ,chemistry ,Reperfusion Injury ,Anesthesia ,Calcium Channels ,Neurology (clinical) ,business ,medicine.drug - Abstract
It is known that mitochondrial ATP-sensitive potassium channels (mitoKATP) play a significant role in protecting cerebral function from ischemia-reperfusion injury, which is related with a decrease in the mitochondrial matrix calcium. However, the effect of mitochondrial calcium uniporter (MCU) on diazoxide-induced cerebral protection is still indistinct. The purpose of the present paper is to further observe the relationship between mitoKATP and MCU, and to probe the mechanism. Adult male Wistar rats were randomly divided into five groups: the Sham group, the I-R group, the Dzx+I-R group, the Dzx+Sper+I-R group, and the Sper+I-R group. Rats not in the Sham group were exposed to 2-hour ischemia followed by 24-hour reperfusion. Diazoxide and spermine were administrated 30 minutes before ischemia or 10 minutes before reperfusion, respectively. After 24-hour reperfusion, animals were given neurological performance tests, overdosed with general anesthesia, and then their brains were excised for infarct volume, pathological changes, and apoptosis analysis. The beneficial effects of diazoxide (improved neurological deficits, decreased infarct volume, and apoptosis, evidenced by the decreased expression of cytochrome c and Bax) were significantly neutralized by spermine. The results of the present work suggest that diazoxide-induced cerebral protection against ischemia-reperfusion injury is mediated by spermine through apoptotic pathway.
- Published
- 2013
40. Lentivirus-mediated LIGHT overexpression inhibits human colorectal carcinoma cell growth in vitro and in vivo
- Author
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Xiang-Ping Liu, Shihai Liu, Haibo Wang, Ruyong Yao, Ai-hua Sui, Chuanzhi Li, Zheng Luo, and Zhuang Yu
- Subjects
Cancer Research ,Herpesvirus entry mediator ,colorectal carcinoma cell ,Oncogene ,Cell growth ,lentiviral vector ,Cell ,Articles ,Cell cycle ,Biology ,Molecular medicine ,Viral vector ,medicine.anatomical_structure ,xenografted tumor ,Oncology ,LIGHT/TNFSF14 ,Immunology ,medicine ,Cancer research ,Tumor necrosis factor alpha - Abstract
Human LIGHT (lymphotoxin-related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells) is the 14th member of the tumor necrosis factor (TNF) superfamily and is therefore also known as TNFSF14. LIGHT has been proven to be a multifunctional molecule affecting cell proliferation, differentiation and a number of other biological processes, in particular, cell growth inhibition. However, the expression and molecular mechanisms of the LIGHT gene in human colorectal carcinoma cells remain largely unclear. In the present study, the LIGHT gene was overexpressed using a lentiviral expression vector in HCT116 human colorectal carcinoma cells in vitro and in vivo, in order to explore the mechanism by which the LIGHT gene inhibits cell growth and suppresses tumor formation. The results showed that the recombinant lentivirus with LIGHT overexpression inhibited the proliferative capacity of the HCT116 cells and significantly decreased the xenografted tumor volumes in nude mice. Furthermore, LIGHT treatment effectively initiated increased caspase-3 and decreased Bcl-2 activities in the HCT116 cells. This study provides a basis for the improved understanding of the role and molecular mechanisms of the LIGHT gene in human colorectal carcinoma cells and may facilitate further functional studies of LIGHT.
- Published
- 2013
41. Overexpressed transcription factor FOXM1 is a potential diagnostic and adverse prognostic factor in postoperational gastric cancer patients
- Author
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Dongfang Tang, Ruyong Yao, Weiwei Qi, Xiaoxiao Li, and Jun Liang
- Subjects
Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Pathology ,Prognostic factor ,Blotting, Western ,Kaplan-Meier Estimate ,Adenocarcinoma ,Gastrectomy ,Stomach Neoplasms ,Transcription (biology) ,Internal medicine ,Biomarkers, Tumor ,medicine ,Forkhead Box ,Humans ,In patient ,Transcription factor ,Proportional Hazards Models ,business.industry ,Forkhead Box Protein M1 ,Forkhead Transcription Factors ,General Medicine ,Middle Aged ,Prognosis ,Immunohistochemistry ,FOXM1 ,Female ,business - Abstract
In the present study, we intend to detect the expression of Forkhead box transcription (FOXM1) in gastric cancer tissues and cell lines, and analyze the correlation between FOXM1 expression and clinic-pathological features as well as their association with clinic outcomes in patients with resectable gastric cancers.We examined the expression of FOXM1 in 103 cancer tissues from patients who underwent gastrectomy during Jan 2007 to Nov 2007 and 68 randomly selected para-cancer tissues by immunohistochemistry. The expression of FOXM1 protein in the benign and malignant human gastric cell lines was simultaneously detected using Western blot analysis. Data on clinic-pathological features and relevant prognostic factors in these patients were then analyzed.FOXM1 expression was absolutely higher in gastric cancer than para-cancer tissues (P 0.001) and normal gastric epithelium cell lines (P = 0.022). No significant association was found between FOXM1 expression and any clinic-pathological parameters (P 0.1). FOXM1 amplification was showed to be independently associated with prognosis in gastric cancer patients (P = 0.001), and its affection is more significant in patients with tumor size larger than 5 cm (P = 0.004), pT3-4 (P = 0.003) or pIII-IV (P = 0.001) as a result of stage-stratified analysis.Overexpressed FOXM1 is a potential diagnostic and poor prognostic biomarker in postoperational gastric cancer patients.
- Published
- 2013
42. Small Interfering RNA (siRNA)-Mediated Silencing of the M2 Subunit of Ribonucleotide Reductase
- Author
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RuYong Yao, Meng Zhang, LiMing Wang, and Jing Wang
- Subjects
Small interfering RNA ,Ribonucleoside Diphosphate Reductase ,endocrine system diseases ,Apoptosis ,RNA interference ,Cell Line, Tumor ,Humans ,Gene silencing ,Medicine ,Gene Silencing ,RNA, Small Interfering ,Ovarian Neoplasms ,Messenger RNA ,business.industry ,Carcinoma ,Cell Cycle ,Obstetrics and Gynecology ,RNA ,Genetic Therapy ,Transfection ,Molecular biology ,RNA silencing ,Oncology ,Drug Resistance, Neoplasm ,Lipofectamine ,Female ,Cisplatin ,business - Abstract
Objective To investigate the effects of small interfering RNA (siRNA)-mediated silencing of the ribonucleotide reductase M2 subunit (RRM2) on the apoptosis and the drug sensitivity of cisplatin-resistant SKOV3/DDP cells. Methods Small interfering RNA transfection was mediated by lipofectamine 2000 to silence RRM2 gene. Messenger RNA (mRNA), and protein expression levels of RRM2 were evaluated by real-time polymerase chain reaction and Western blot after transfection. The cell growth inhibition rate was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The cellular apoptosis and cycling was identified by flow cytometry (FCM). Results The messenger RNA and protein expression levels of RRM2 markedly decreased after the RRM2 siRNA transfection. The half inhibition concentration of cisplatin in RRM2-RNA interference cells (interference group) was lower than that in RRM2-negative cells (noninterference group) and the SKOV3/DDP cells (blank control group) (P = 0.032). Small interfering RNA–mediated inhibition of RRM2 effectively induced G1/S-phase cell cycle arrest and increased drug (gemcitabine and cisplatin)-induced apoptotic fraction at 72 hours ( (96% ± 3.0)%) after transfection (P Conclusion Small interfering RNA–mediated RRM2 knockdown significantly reversed SKOV3/DDP cell resistance to cisplatin. RNA interference technology combined with gemcitabine and cisplatin can effectively improve the apoptosis rate of the cisplatin-resistant ovarian cancer cell, which is expected to become the first-line treatment options for the cisplatin-resistant ovarian cancer.
- Published
- 2013
43. Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene
- Author
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Ruyong Yao, Xiangping Liu, Zhen Wang, Ai-Hua Sui, Shihai Liu, Wensheng Qiu, Hongsheng Yu, and Lu Yue
- Subjects
Cancer Research ,Epidemiology ,IER3 ,Genetic Vectors ,Green Fluorescent Proteins ,Gene Expression ,Biology ,Transfection ,medicine.disease_cause ,Polymerase Chain Reaction ,Green fluorescent protein ,Plasmid ,Genes, Reporter ,Cell Line, Tumor ,Gene expression ,medicine ,Humans ,Gene ,Expression vector ,Public Health, Environmental and Occupational Health ,Membrane Proteins ,DNA Restriction Enzymes ,Molecular biology ,Oncology ,Apoptosis Regulatory Proteins ,Carcinogenesis ,Plasmids - Abstract
Background: More and more research indicate that the immediately early response gene 3 (IER3) is involved inmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression. Methods: Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Westernblotting. Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.
- Published
- 2013
44. Differential expression of the Na+/I− symporter protein in thyroid cancer and adjacent normal and nodular goiter tissues
- Author
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Yansong Lin, Jun Liang, Ruyong Yao, and Shasha Wang
- Subjects
endocrine system ,Cancer Research ,medicine.medical_specialty ,Pathology ,Goiter ,endocrine system diseases ,Oncogene ,business.industry ,Cancer ,Articles ,medicine.disease ,Epithelium ,Thyroid carcinoma ,Endocrinology ,medicine.anatomical_structure ,Oncology ,Internal medicine ,Symporter ,medicine ,Immunohistochemistry ,business ,Thyroid cancer ,health care economics and organizations - Abstract
The ability of differentiated thyroid cancer and adjacent thyroid cells to concentrate iodine is dependent on their expression of a functional NA(+)/I(-) symporter (NIS). Thyroid cancer is insensitive to (131)I treatment if the thyroid cells lack the ability to concentrate iodide. Thus, in this study, we aimed to determine whether the NIS protein was differentially expressed in thyroid cancer and various surrounding tissues. We recruited 114 cases of papillary thyroid carcinoma (PTC) and divided them into two groups: 60 patients of 9 males and 51 females with a mean age of 49.55 years who had PTC with surrounding nodular goiter tissue (simplified as G(NG)), and 54 patients of 8 males and 46 females with a mean age of 45.78 years who had PTC with surrounding normal tissue (G(normal)) after total or near total thyroidectomy. Formalin-fixed and paraffin-embedded tissue sections were prepared for immunohistochemical staining of the NIS protein and semi-quantitative analysis. The NIS protein was expressed in the basolateral membrane of the normal epithelium, while PTC and nodular goiter cells expressed NIS in the cytoplasm and basolateral membrane. The expression levels of the NIS protein were higher in the adjacent normal tissues compared with those of the surrounding nodular goiter tissues (P=0.002) and expression levels of the NIS protein were higher in PTC tissues compared with the surrounding nodular goiter tissues (P=0.008). The data from this study indicate that cancer-surrounding tissues may play a significant role in mediating the sensitivity of PTC patients to radioactive iodine treatment.
- Published
- 2012
45. Induction of non-small cell lung carcinoma apoptosis using soluble RGD-TRAIL by targeting the integrin receptor of tumor cells
- Author
-
Xiangping Liu, Haiping Zhang, Shihai Liu, Ruyong Yao, Aihua Sui, Zhenli Wang, and Quan Zhou
- Subjects
Cancer Research ,Lung Neoplasms ,CD30 ,Cell Survival ,Recombinant Fusion Proteins ,Cell ,Antineoplastic Agents ,Apoptosis ,Biology ,Biochemistry ,TNF-Related Apoptosis-Inducing Ligand ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Escherichia coli ,Genetics ,medicine ,Humans ,Receptors, Vitronectin ,Molecular Biology ,Oncogene ,Cell growth ,Cell cycle ,Integrin alphaVbeta3 ,medicine.anatomical_structure ,Oncology ,Cell culture ,Cancer research ,Molecular Medicine ,Tumor necrosis factor alpha ,Poly(ADP-ribose) Polymerases ,Oligopeptides ,A431 cells - Abstract
The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for cancer therapeutics that exhibits the ability to preferentially induce apoptosis in malignant cells. RGD peptides bind to the integrins, ανβ3 and ανβ5, which are selectively expressed in tumor neovasculature and at the surface of certain tumor cells. To enhance the antitumor effect, an RGD-TRAIL protein, in which TRAIL was fused with the RGD motif-containing cell adhesive sequence, GRGDNP (gly-arg-gly-asp-asn-pro), was constructed and evaluated for bioactivity. The soluble TRAIL and RGD-TRAIL proteins were expressed in Escherichia coli BL21 (DE3) and purified using a non-denaturing method. The antitumor effect of the purified RGD-TRAIL on cell proliferation was evaluated in vitro using MTT and wound healing assays and cell apoptosis was assessed by Hoechst 33342 staining and PARP expression analysis. The results revealed that RGD-TRAIL inhibited the proliferation of multiple tumor cell lines (A549, NCI-H1299 and HCC827). Western blot analysis demonstrated that the treatment of tumor cells with RGD-TRAIL activated the apoptotic pathway by the cleavage of PARP, in the same way as wild-type TRAIL (wtTRAIL). These results demonstrate that RGD-TRAIL possesses more potent antitumor effects than wtTRAIL and, therefore, merits further investigation in preclinical and clinical studies.
- Published
- 2012
46. Effects of transfecting Bcl-2 shRNA and celecoxb on the SGC-7901 cell line
- Author
-
Minggang Lin, Wenjing Xiao, Aiqin Wang, Ruyong Yao, Fangzhen Shen, and Weiwei Liu
- Subjects
musculoskeletal diseases ,organic chemicals ,Biology ,Molecular biology ,In vitro ,Small hairpin RNA ,Oncology ,Surgical oncology ,Cell culture ,Celecoxib ,medicine ,lipids (amino acids, peptides, and proteins) ,heterocyclic compounds ,skin and connective tissue diseases ,medicine.drug - Abstract
Objective The aim of this study was to study the changes of SGC-7901 cells transfecting small hairpin RNA (shRNA) targeted Bcl-2 and celecoxib in vitro. (shRNA) targeted Bcl-2 and celecoxib in vitro.
- Published
- 2012
47. Mechanism of RNA interference targeting at survivin gene on apoptosis of hepatoma-cellular carcinoma cell line HepG2
- Author
-
Wei Lei, Shanai Song, Weiwei Qi, Jian Jiang, Hongsheng Yu, Yali Miao, Ruyong Yao, and Dongyu Hu
- Subjects
Mechanism (biology) ,Biology ,medicine.disease ,Molecular biology ,digestive system diseases ,Carcinoma cell line ,Oncology ,Surgical oncology ,Apoptosis ,RNA interference ,Hepatocellular carcinoma ,Survivin ,medicine ,Cancer research ,Gene - Abstract
Objective The aim of this study was to investigate the molecular mechanism of anti-apoptotic action of survivin to the hepatoma-cellular carcinoma cell line HepG2.
- Published
- 2011
48. The combination of ERCC1 and XRCC1 gene polymorphisms better predicts clinical outcome to oxaliplatin-based chemotherapy in metastatic colorectal cancer
- Author
-
Jun Liang, Weiwei Qi, Hongying Lv, Zimin Liu, Ruyong Yao, and Tao Jiang
- Subjects
Adult ,Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Genotype ,Organoplatinum Compounds ,Colorectal cancer ,medicine.medical_treatment ,Toxicology ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Metastasis ,XRCC1 ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Pharmacology (medical) ,Lymphocytes ,Neoplasm Metastasis ,Codon ,Aged ,Retrospective Studies ,XRCC1 Gene ,Pharmacology ,Chemotherapy ,business.industry ,Cancer ,Middle Aged ,Endonucleases ,medicine.disease ,Oxaliplatin ,Surgery ,DNA-Binding Proteins ,Survival Rate ,Treatment Outcome ,X-ray Repair Cross Complementing Protein 1 ,Disease Progression ,Female ,ERCC1 ,Colorectal Neoplasms ,business ,medicine.drug - Abstract
To evaluate the effect of excision repair cross-complementing group 1 (ERCC1) and X-ray cross-complementing group 1 (XRCC1) gene polymorphisms on treatment outcome in patients receiving oxaliplatin-based regimens for metastatic colorectal cancer.Hundred and thirteen patients with a diagnosis of metastatic colorectal cancer were treated with oxaliplatin-based chemotherapy. ERCC1 codon 118C/T and XRCC1 codon 399A/G polymorphisms were tested by real-time polymerase chain reaction (RT-PCR) method in peripheral blood lymphocytes of these patients. Disease control rates and survivals were compared by types of genotypes.Analyses of the patterns of the polymorphism located at ERCC1 codon 118 showed that 55 (48.67%) patients were homozygous for C/C genotype, 15 (13.27%) were homozygous for the T/T genotype, and 43 (38.06%) were heterozygous for C/T genotype. Analyses of the polymorphism located at XRCC1 codon 399 showed that 61 (53.98%) patients were homozygous for A/A genotype, 13 (11.50%) were homozygous for the G/G genotype, and 39 (34.52%) were heterozygous for A/G genotype. After two cycles of chemotherapy, there was complete response (CR) in 1 patient, partial response (PR) in 24 patients, and stable disease (SD) in 56 patients. Altogether in 81 (71.68%) patients the disease was controlled after chemotherapy. Thirty-two (28.32%) patients showed disease progression. After adjusting for some clinical factors, both the ERCC1 polymorphism and the XRCC1 polymorphism lost their roles in predicting DCR (P = 0.662, P = 0.631) and MST (P = 0.692, P = 0.572). But the combination of ERCC1 and XRCC1 polymorphisms was significantly associated with DCR (P = 0.01) and MST (P = 0.000) independently.This is the first study which showed that polymorphisms of ERCC1 and XRCC1, in combination not individually, were independent predictors for DCR and OS. This may contribute to the selection of patients who would benefit from oxaliplatin-based chemotherapy for metastatic colorectal cancer.
- Published
- 2009
49. ERCC1 Asn118Asn polymorphism as predictor for cancer response to oxaliplatin-based chemotherapy in patients with advanced colorectal cancer
- Author
-
Gang Wu, Jun Liang, Ruyong Yao, Hua Liang, and Hongying Lv
- Subjects
Oncology ,medicine.medical_specialty ,Chemotherapy ,business.industry ,medicine.medical_treatment ,Chemotherapy regimen ,Oxaliplatin ,Surgical oncology ,Internal medicine ,Genotype ,medicine ,ERCC1 ,business ,Allele frequency ,Genotyping ,medicine.drug - Abstract
To assess whether the polymorphism of ERCC1 Asn118Asn (C → T) had effects on cancer response to chemotherapy and outcome in Chinese patients treated with oxaliplatin as first-line chemotherapy regimen for advanced colorectal cancer. ERCC1 Asn118Asn polymorphism was analyzed in 99 patients with stages III and IV advanced colorectal cancer treated with oxaliplatin-based chemotherapy. For all of the patients, ERCC1 Asn118Asn genotype was analyzed for associations with treatment response and time to disease progress (TTP). The allele frequencies of the ERCC1 gene codon 118 were C/C 50.51% (50/99), C/T 41.41% (41/99), T/T 8.08% (8/99), respectively. Patients with C/C genotype showed higher response rate than those with C/T + T/T (OR = 3.764, 95% CI: 1.310–10.813). The median TTP of all patients was 7 months (95% CI: 5.569–8.431). Patients with C/C genotype showed a median TTP of 10 months (95% CI: 8.924–11.076), which was longer than 5 months (95% CI: 4.424–5.576) in patients with C/T + T/T genotypes. Our results showed a link between ERCC1 Asn118Asn genetic polymorphism and cancer response to oxaliplatin-based chemotherapy and time to disease progress in Chinese patients with advanced colorectal cancer. ERCC1 Asn118Asn genotyping may be of predictive benefit in selecting treatment regimen for advanced colorectal cancer.
- Published
- 2008
50. Polymorphisms of ERCC1 and XRCC1 predict the overall survival of advanced gastric cancer patients receiving oxaliplatin-based chemotherapy
- Author
-
Lijian, Zhang, Ruyong, Yao, Shibao, Fang, Xiuwen, Wang, and Xin, Li
- Subjects
Original Article - Abstract
The aim of the present study was to evaluate the clinical outcome of excision repair cross-complementing protein 1 (ERCC1) and X-ray repair cross-complementing protein 1 (XRCC1) gene polymorphisms in 89 patients receiving oxaliplatin/5-fluorouracil-based chemotherapy as a first-line treatment regimen for advanced gastric cancer. ERCC1 codon 118C/T and XRCC1 codon 399A/G polymorphisms were identified using quantitative polymerase chain reactions, and the associations between disease control rate (DCR), median overall survival (mOS) and gene polymorphisms were analyzed. Following two cycles of chemotherapy, a complete response was observed in two patients, a partial response in 18 patients, stable disease in 38 patients and progressive disease in 31 patients. It was determined that ERCC1 and XRCC1 polymorphisms are not associated with DCR (P=0.662 and P=0.631, respectively). The mOS of patients exhibiting ERCC1 and XRCC1 polymorphisms was eight months, and although no significant association was identified between ERCC1 codon 118 genotypes and mOS (P>0.05), the combination of ERCC1 and XRCC1 polymorphisms, as well as the specific presence of the XRCC1 codon 399A/G polymorphism, was associated with mOS (P
- Published
- 2015
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