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In this work, we studied the biochemical characteristics of the diatom Cylindrotheca closterium in the cumulative mode of cultivation in different phases of growth. According to our data, in the exponential growth phase, C. closterium biomass is characterized by a minimum lipid content of 3% of dry biomass and a maximum carbohydrates content of 23% of dry weight. During the transition of C. closterium culture to the stationary growth phase, as in the case of a representative of diatoms, a rather high accumulation of total lipids is observed – to 28% dry weight, while the carbohydrates content decreases to 7%. At the end of the stationary phase (21st day of cultivation), the content of fatty acids is 50% of total lipids or 12.5% of dry weight. A certain relationship between the concentrations of fucoxanthin, total lipids and polyunsaturated fatty acids (PUFA) in the biomass of C. сlosterium diatom was recorded. During active growth in the exponential phase, the concentration of fucoxanthin was 0.5–1 mg∙g-1 dry weight. During the tranition of the culture to the stationary phase of growth, the concentration of fucoxanthin reached 7±0.2 mg∙g-1 dry biomass. At the end of the stationary phase, the concentration of fucoxanthin reached its maximum value of 20 mg∙g-1 dry weight.

This work described toxicity of composition of fucoxanthin with particles of porous aluminum oxide with polydimethylsiloxane (Fx@Al/PDMS) in mice lymphocytes. Effects of Fx@Al/PDMS on Balb/c mice splenocytes and thymocytes viability, and proliferation, and intracellular myeloperoxidase activity, and nitric oxide (NO) production, and apoptosis were studied. It was shown that short-term (24 h) and long-term (129 h) incubation lymphocytes with Fx@Al/PDMS did not have any adverse effect on functional properties of splenocytes and thymocytes.

Growth characteristics of a diatom microalgе Cylindrotheca closterium are investigated. Cylindrotheca closterium cultivated on nutrient mediums which were received by proportional increase all biogenous elements concentration of the environment F. It is shown that culture efficiency increases due to proportional increase of the biogenous elements up to 20 F in a nutrient medium. The maximum efficiency of this microalga is received at cultivation on a nutrient medium 20 F and made 1.74 g/l.