11 results on '"Rushing JS"'
Search Results
2. The ART of cryopreservation and its changing landscape.
- Author
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Pomeroy KO, Comizzoli P, Rushing JS, Lersten IL, and Nel-Themaat L
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- Cryopreservation trends, Fertility Preservation trends, Germ Cells physiology, Germ Cells transplantation, Humans, Vitrification, Cryopreservation methods, Fertility Preservation methods, Reproductive Techniques, Assisted trends
- Abstract
The purpose of this review is to educate the reader on the role that cryopreservation has played and continues to play in the ever-evolving field of assisted reproductive technologies, specifically in clinical human fertility treatment. We discuss the science behind the cryopreservation methods and investigated some of the major considerations that any clinic or cryobank faces in terms of risks and liabilities, physical challenges that accompany the constantly growing collection of cryopreserved specimens, and what this means on the ethical and legal front. Finally, we take a glimpse in the future to explore what may be on the horizon for the preservation of gametes and reproductive tissues., (Copyright © 2022. Published by Elsevier Inc.)
- Published
- 2022
- Full Text
- View/download PDF
3. Pathophysiology and management of classic galactosemic primary ovarian insufficiency.
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Hagen-Lillevik S, Rushing JS, Appiah L, Longo N, Andrews A, Lai K, and Johnson J
- Subjects
- Animals, Disease Models, Animal, Female, Fertility, Galactose, Humans, Mice, Ovary, Galactosemias
- Abstract
Classic galactosemia is an inborn error of carbohydrate metabolism associated with early-onset primary ovarian insufficiency (POI) in young women. Our understanding of the consequences of galactosemia upon fertility and fecundity of affected women is expanding, but there are important remaining gaps in our knowledge and tools for its management, and a need for continued dialog so that the special features of the condition can be better managed. Here, we review galactosemic POI and its reproductive endocrinological clinical sequelae and summarize current best clinical practices for its management. Special consideration is given to the very early-onset nature of the condition in the pediatric/adolescent patient. Afterward, we summarize our current understanding of the reproductive pathophysiology of galactosemia, including the potential action of toxic galactose metabolites upon the ovary. Our work establishing that ovarian cellular stress reminiscent of endoplasmic reticulum (ER) stress is present in a mouse model of galactosemia, as well as work by other groups, are summarized., Lay Summary: Patients with the condition of classic galactosemia need to maintain a strict lifelong diet that excludes the sugar galactose. This is due to having mutations in enzymes that process galactose, resulting in the buildup of toxic metabolic by-products of the sugar. Young women with classic galactosemia often lose the function of their ovaries very early in life (termed 'primary ovarian insufficiency'), despite adherence to a galactose-restricted diet. This means that in addition to the consequences of the disease, these women also face infertility and the potential need for hormone replacement therapy. This article summarizes current strategies for managing the care of galactosemic girls and women and also what is known of how the condition leads to early primary ovarian insufficiency., (© The authors.)
- Published
- 2021
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4. Fertility Issues in Polycystic Ovarian Disease: A Systematic Approach.
- Author
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Rushing JS and Santoro N
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- Clomiphene, Female, Fertility, Fertility Agents, Female, Humans, Infertility, Female etiology, Polycystic Ovary Syndrome complications
- Abstract
The triad of hirsutism, amenorrhea, and enlarged polycystic ovaries first was described in 1935 and later become known as polycystic ovarian syndrome (PCOS). Women with PCOS are more likely to have cardiometabolic challenges that also have an indirect relationship to their fertility and fertility outcomes. Despite these challenges, their fertile life span appears to be longer. Ovulation induction is considered first-line management of infertility in women with PCOS, with letrozole superior to clomiphene. Women with PCOS undergoing in vitro fertilization are high risk for ovarian hyperstimulation syndrome but also have a higher live birth rate compared with controls., Competing Interests: Disclosure The authors have nothing to disclose., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
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5. Ovarian stimulation for fertility preservation in an oncology patient with etonogestrel implant in place.
- Author
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Rushing JS, Appiah L, Polotsky AJ, Murray S, Foust E, Hassell K, and Roeca C
- Subjects
- Adult, Anti-Mullerian Hormone administration & dosage, Cryopreservation, Female, Follicle Stimulating Hormone administration & dosage, Gonadotropin-Releasing Hormone administration & dosage, Humans, Infertility, Female etiology, Infertility, Female metabolism, Infertility, Female pathology, Luteinizing Hormone administration & dosage, Neoplasms pathology, Oocyte Retrieval methods, Oocytes drug effects, Oocytes growth & development, Oogenesis drug effects, Oogenesis genetics, Ovarian Hyperstimulation Syndrome, Ovulation Induction methods, Prostheses and Implants adverse effects, Vitrification, Desogestrel administration & dosage, Fertility Preservation, Infertility, Female drug therapy, Neoplasms complications
- Abstract
Purpose: To describe a case of a young woman who presented for fertility preservation and underwent ovarian stimulation with an etonogestrel implant in place., Methods: A 24-year old, gravida 0, with an etonogestrel implant and newly diagnosed lower extremity sarcoma and DVT desiring oocyte cryopreservation prior to adjuvant chemotherapy and radiation. To avoid delay in her oncologic care and allow for continued use of contraception post-retrieval, the patient underwent controlled ovarian hyperstimulation (COH) without removal of the etonogestrel implant., Results: Baseline labs included follicle-stimulating hormone 9 mIU/mL, luteinizing hormone 4.9 mIU/mL, estradiol 42 pg/mL, anti-Müllerian hormone 5.1 ng/mL, and antral follicle count greater than 40. The patient was placed on an antagonist protocol and stimulated with 125 IU Gonal-F and 75 IU Menopur. She received a total of 12 days of gonadotropin stimulation. On the day of trigger, her estradiol was 1472 pg/mL, lead follicle 21.5 mm with a total of 25 follicles measured > 12 mm. She was triggered with 5000 U hCG. She had a total of 23 oocytes retrieved, 17 of which were metaphase II and vitrified., Conclusions: COH and successful oocyte cryopreservation can be achieved in patients with an etonogestrel implant in situ without apparent detrimental effects to oocyte yield or maturity. Due to the etonogestrel implant's inhibitory effects on LH, it is recommended to use an hCG trigger for final oocyte maturation.
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- 2021
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6. A Mentorship Program for Academic Obstetrician Gynecologists that Improved Publication and Overall Confidence for Success.
- Author
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Nasab S, Rushing JS, Segars JH, Evers E, Handa VL, Lawson S, Miller C, Yenokyan G, Bienstock J, and Satin AJ
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- Curriculum, Female, Health Personnel, Humans, Leadership, Pregnancy, Mentors, Physicians
- Abstract
This article aims to evaluate the impact of a mentorship program to enhance the training of clinical and research scientists in obstetrics and gynecology (OBGYN). A departmental course was developed for junior faculty and fellows based on their areas of interest. The research was IRB-approved. The curriculum consisted of monthly interactive workshops for an interdisciplinary group of trainees in OBGYN. Themes included research, education, and leadership in academic OBGYN. There was a strong emphasis on participatory exercises. Examples of curriculum topics included manuscript publication and review, grant writing, working with an IRB, promotion, and time management. Pre- and post-course questionnaires assessed participants' confidence in skills related to the course topics. Generalized linear models were used to assess changes in post-course response, using each question as the dependent variable and an indicator for post-course as the predictor variable. The control group was composed of junior faculty and fellows before the course was initiated. Outcome measures included the number and impact factor of published manuscripts. A Wilcoxon rank-sum test was used to assess outcome measures. Of the 118 attendees, 26 (22.0%) were junior faculty, 35 (29.66%) were clinical fellows, and 28 (23.7%) were research fellows, other research staff, or students. For each 3-year course series, an average of 20 participants completed the post-course surveys, of which 72% were clinical fellows, 22% were assistant professors, and 5% were instructors. The data revealed a statistically significant change in the participant's overall confidence in skills related to research, education, and leadership when comparing the cumulative results from the pre-to-post course surveys ( p < 0.001). Specifically, participants noted improved confidence in their skills related to clinical and translation research ( p < 0.001) and leadership and academic career advancement ( p = 0.001). Additionally, junior faculty and clinical fellows who attended the course had a higher number of publications during the course period compared with controls ( p = 0.003 and p = 0.008, respectively). This subspecialty-tailored, departmental training program was effective in increasing junior faculty and clinical fellows' confidence in skills related to career advancement and research and in the number of peer-reviewed publications., Competing Interests: Disclosure The authors report no conflicts of interest in this work., (Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.)
- Published
- 2019
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7. Role of Shp2 in forebrain neurons in regulating metabolic and cardiovascular functions and responses to leptin.
- Author
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do Carmo JM, da Silva AA, Sessums PO, Ebaady SH, Pace BR, Rushing JS, Davis MT, and Hall JE
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- Animals, Blood Pressure, Body Temperature, Calorimetry, Indirect, Eating, Heart Rate, Intracellular Signaling Peptides and Proteins, Male, Mice, Mice, Transgenic, Neurons, Obesity, Oxygen Consumption, Signal Transduction, Energy Metabolism, Leptin metabolism, Prosencephalon pathology, Protein Tyrosine Phosphatase, Non-Receptor Type 11 deficiency, Receptors, Leptin metabolism
- Abstract
Objective: We examined whether deficiency of Src homology 2 containing phosphatase (Shp2) signaling in forebrain neurons alters metabolic and cardiovascular regulation under various conditions and if it attenuates the anorexic and cardiovascular effects of leptin. We also tested whether forebrain Shp2 deficiency alters blood pressure (BP) and heart rate (HR) responses to acute stress., Design: Forebrain Shp2(-/-) mice were generated by crossing Shp2(flox/flox) mice with CamKIIα-cre mice. At 22-24 weeks of age, the mice were instrumented for telemetry for measurement of BP, HR and body temperature (BT). Oxygen consumption (VO2), energy expenditure and motor activity were monitored by indirect calorimetry., Results: Shp2/CamKIIα-cre mice were heavier (46±3 vs 32±1 g), hyperglycemic, hyperleptinemic, hyperinsulinemic and hyperphagic compared to Shp2(flox/flox) control mice. Shp2/CamKIIα-cre mice exhibited reduced food intake responses to fasting/refeeding and impaired regulation of BT when exposed to 15 and 30 °C ambient temperatures. Despite being obese and having many features of metabolic syndrome, Shp2/CamKIIα-cre mice had similar daily average BP and HR compared to Shp2(flox/flox) mice (112±2 vs 113±1 mm Hg and 595±34 vs 650±40 b.p.m.), but exhibited increased BP and HR responses to cold exposure and acute air-jet stress test. Leptin's ability to reduce food intake and to raise BP were markedly attenuated in Shp2/CamKIIα-cre mice., Conclusion: These results suggest that forebrain Shp2 signaling regulates food intake, appetite responses to caloric deprivation and thermogenic control of body temperature during variations in ambient temperature. Deficiency of Shp2 signaling in the forebrain is associated with augmented cardiovascular responses to cold and acute stress but attenuated BP responses to leptin.
- Published
- 2014
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8. Differential control of metabolic and cardiovascular functions by melanocortin-4 receptors in proopiomelanocortin neurons.
- Author
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do Carmo JM, da Silva AA, Rushing JS, Pace B, and Hall JE
- Subjects
- Adipose Tissue, Brown metabolism, Adiposity drug effects, Animals, Blood Glucose metabolism, Blood Pressure, Body Temperature, Brain drug effects, Eating, Glucose Tolerance Test, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Heart Rate, Infusions, Intraventricular, Integrases genetics, Integrases metabolism, Intra-Abdominal Fat metabolism, Ion Channels metabolism, Male, Melanocyte-Stimulating Hormones administration & dosage, Mice, Mice, Knockout, Mice, Transgenic, Mitochondrial Proteins metabolism, Monitoring, Ambulatory methods, Neurons drug effects, Oxygen Consumption, Peptides, Cyclic administration & dosage, Receptor, Melanocortin, Type 4 agonists, Receptor, Melanocortin, Type 4 deficiency, Receptor, Melanocortin, Type 4 genetics, Recombinant Fusion Proteins metabolism, Telemetry, Time Factors, Uncoupling Protein 1, Weight Gain, alpha-MSH administration & dosage, alpha-MSH analogs & derivatives, Brain metabolism, Energy Metabolism drug effects, Hemodynamics drug effects, Neurons metabolism, Pro-Opiomelanocortin metabolism, Receptor, Melanocortin, Type 4 metabolism, Stress, Physiological
- Abstract
We examined the role of melanocortin-4 receptors (MC4R) in proopiomelanocortin (Pomc) neurons in regulating metabolic and cardiovascular functions. Using Cre-loxP technology, we selectively rescued MC4R in Pomc neurons of mice with whole body MC4R deficiency (MC4R-Pomc-Cre mice). Body weight, food intake, and whole body oxygen consumption (Vo2) were determined daily, and blood pressure (BP), heart rate (HR), and body temperature were measured 24 h/day by telemetry. An intracerebroventricular cannula was placed in the right lateral ventricle for intracerebroventricular infusions. Littermate MC4R-deficient (LoxTB-MC4R) mice were used as controls. After control measurements, the MC4R antagonist (SHU-9119; 1 nmol/h) was infused intracerebroventricularly for 7 days. Compared with LoxTB-MC4R mice, MC4R-Pomc-Cre mice were less obese (47 ± 2 vs. 52 ± 2 g) and had increased energy expenditure (2,174 ± 98 vs. 1,990 ± 68 ml·kg⁻¹·min⁻¹), but food intake (4.4 ± 0.2 vs. 4.3 ± 0.3 g/day), BP (112 ± 1 vs. 109 ± 3 mmHg), and HR [557 ± 9 vs. 551 ± 14 beats per minute (bpm)] were similar between groups. Chronic SHU-9119 infusion increased food intake (4.2 ± 0.2 to 6.1 ± 0.5 g/day) and body weight (47 ± 2 to 52 ± 2 g) in MC4R-Pomc-Cre mice, while no changes were observed in LoxTB-MC4R mice. Chronic SHU-9119 infusion also increased BP and HR by 5 ± 1 mmHg and 60 ± 8 bpm in MC4R-Pomc-Cre mice without altering BP or HR in LoxTB-MC4R mice. These results indicate that MC4Rs in Pomc neurons are important for regulation of energy balance. In contrast, while activation of MC4R in Pomc neurons facilitates the BP response to acute stress, our data do not support a major role of MC4R in Pomc neurons in regulating baseline BP and HR.
- Published
- 2013
- Full Text
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9. Activation of the central melanocortin system contributes to the increased arterial pressure in obese Zucker rats.
- Author
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do Carmo JM, da Silva AA, Rushing JS, and Hall JE
- Subjects
- Animals, Blood Glucose metabolism, Disease Models, Animal, Heart Rate physiology, Infusions, Intraventricular, Insulin blood, Male, Melanocyte-Stimulating Hormones administration & dosage, Melanocyte-Stimulating Hormones pharmacology, Obesity blood, Rats, Rats, Zucker, Receptor, Melanocortin, Type 3 antagonists & inhibitors, Receptor, Melanocortin, Type 3 drug effects, Receptor, Melanocortin, Type 4 antagonists & inhibitors, Receptor, Melanocortin, Type 4 drug effects, Receptors, Leptin physiology, Blood Pressure physiology, Central Nervous System physiology, Hypertension physiopathology, Melanocortins physiology, Obesity physiopathology
- Abstract
We have previously demonstrated that leptin-mediated activation of the central nervous system (CNS) melanocortin system reduces appetite and increases sympathetic activity and blood pressure (BP). In the present study we examined whether endogenous melanocortin system activation, independent of leptin's actions, contributes to the regulation of BP and metabolic functions in obese Zucker rats, which have mutated leptin receptors. The long-term cardiovascular and metabolic effects of central melanocortin-3/4 receptor (MC3/4R) antagonism with SHU-9119 were assessed in lean (n = 6) and obese (n = 8) Zucker rats. BP and heart rate (HR) were measured 24-h/day by telemetry and an intracerebroventricular cannula was placed in the brain lateral ventricle. After stable control measurements, SHU-9119 was infused intracerebroventricularlly (1 nmol/h) for 10 days followed by a 10-day recovery period. Chronic CNS MC3/4R antagonism significantly increased food intake and body weight in lean (20 ± 1 to 45 ± 2 g and 373 ± 11 to 432 ± 14 g) and obese (25 ± 2 to 35 ± 2 g and 547 ± 10 to 604 ± 11 g) rats. No significant changes were observed in plasma glucose levels in lean or obese rats, whereas plasma leptin and insulin levels markedly increased in lean Zucker rats during CNS MC3/4R antagonism. Chronic SHU-9119 infusion in obese Zucker rats reduced mean arterial pressure (MAP) and HR by 6 ± 1 mmHg and 24 ± 5 beats/min, whereas in lean rats SHU-9119 infusion reduced HR by 31 ± 9 beats/min while causing only a transient decrease in MAP. These results suggest that in obese Zucker rats the CNS melanocortin system contributes to elevated BP independent of leptin receptor activation.
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- 2012
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10. The role of the Internet in systems integration.
- Author
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Rushing JS, Lafrance L, Daou G, Nutkis DS, Weitzel R, Shay M, Patton P, McCord JR, Demetriades JT, and Morrison T
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- Software, United States, Computer Communication Networks organization & administration, Systems Integration
- Published
- 1996
11. Reform demands strategic action at the regional level.
- Author
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Rushing JS
- Subjects
- Community Participation, Data Collection, Europe, Health Care Costs statistics & numerical data, Health Care Costs trends, Information Systems, Managed Care Programs, Outcome Assessment, Health Care organization & administration, Outcome Assessment, Health Care statistics & numerical data, United States, Health Care Reform trends, Regional Health Planning organization & administration
- Abstract
Those who understand the primary forces driving reform are in a better position to survive the transformation underway. To succeed, organizations must use this knowledge and act strategically at the regional level.
- Published
- 1994
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