18 results on '"Rupesh Kumar, Mishra"'
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2. Design and Implementation of Brain Tumor Segmentation and Detection Using a Novel Woelfel Filter and Morphological Segmentation.
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M. Venu Gopalachari, Morarjee Kolla, Rupesh Kumar Mishra, and Zarin Tasneem
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- 2022
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3. Optical Biosensors for Diagnostics of Infectious Viral Disease: A Recent Update
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Atul Sharma, Rupesh Kumar Mishra, K. Yugender Goud, Mona A. Mohamed, Shekher Kummari, Swapnil Tiwari, Zhanhong Li, Roger Narayan, Lia A. Stanciu, and Jean Louis Marty
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viral disease ,optical biosensors ,diagnostics ,nanomaterial ,fluorescence ,SARS-CoV-2 ,Medicine (General) ,R5-920 - Abstract
The design and development of biosensors, analytical devices used to detect various analytes in different matrices, has emerged. Biosensors indicate a biorecognition element with a physicochemical analyzer or detector, i.e., a transducer. In the present scenario, various types of biosensors have been deployed in healthcare and clinical research, for instance, biosensors for blood glucose monitoring. Pathogenic microbes are contributing mediators of numerous infectious diseases that are becoming extremely serious worldwide. The recent outbreak of COVID-19 is one of the most recent examples of such communal and deadly diseases. In efforts to work towards the efficacious treatment of pathogenic viral contagions, a fast and precise detection method is of the utmost importance in biomedical and healthcare sectors for early diagnostics and timely countermeasures. Among various available sensor systems, optical biosensors offer easy-to-use, fast, portable, handy, multiplexed, direct, real-time, and inexpensive diagnosis with the added advantages of specificity and sensitivity. Many progressive concepts and extremely multidisciplinary approaches, including microelectronics, microelectromechanical systems (MEMSs), nanotechnologies, molecular biology, and biotechnology with chemistry, are used to operate optical biosensors. A portable and handheld optical biosensing device would provide fast and reliable results for the identification and quantitation of pathogenic virus particles in each sample. In the modern day, the integration of intelligent nanomaterials in the developed devices provides much more sensitive and highly advanced sensors that may produce the results in no time and eventually help clinicians and doctors enormously. This review accentuates the existing challenges engaged in converting laboratory research to real-world device applications and optical diagnostics methods for virus infections. The review’s background and progress are expected to be insightful to the researchers in the sensor field and facilitate the design and fabrication of optical sensors for life-threatening viruses with broader applicability to any desired pathogens.
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- 2021
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4. CNN-Based Brain Tumor Detection Model Using Local Binary Pattern and Multilayered SVM Classifier
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Morarjee Kolla, Rupesh Kumar Mishra, S Zahoor ul Huq, Y. Vijayalata, M Venu Gopalachari, and KazyNoor-e-Alam Siddiquee
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Benchmarking ,Engineering ,Support Vector Machine ,Article Subject ,General Computer Science ,Brain Neoplasms ,General Mathematics ,General Neuroscience ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Humans ,General Medicine ,Algorithms - Abstract
In this paper, an autonomous brain tumor segmentation and detection model is developed utilizing a convolutional neural network technique that included a local binary pattern and a multilayered support vector machine. The detection and classification of brain tumors are a key feature in order to aid physicians; an intelligent system must be designed with less manual work and more automated operations in mind. The collected images are then processed using image filtering techniques, followed by image intensity normalization, before proceeding to the patch extraction stage, which results in patch extracted images. During feature extraction, the RGB image is converted to a binary image by grayscale conversion via the colormap process, and this process is then completed by the local binary pattern (LBP). To extract feature information, a convolutional network can be utilized, while to detect objects, a multilayered support vector machine (ML-SVM) can be employed. CNN is a popular deep learning algorithm that is utilized in a wide variety of engineering applications. Finally, the classification approach used in this work aids in determining the presence or absence of a brain tumor. To conduct the comparison, the entire work is tested against existing procedures and the proposed approach using critical metrics such as dice similarity coefficient (DSC), Jaccard similarity index (JSI), sensitivity (SE), accuracy (ACC), specificity (SP), and precision (PR).
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- 2022
5. In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii
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Maneesh MISHRA, Syed Uzma JALIL, Rupesh Kumar MISHRA, Swati KUMARI, and Brajesh Kumar PANDEY
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chitinase ,genetic transformation ,pathogen inhibition assay ,quantification of chitinase ,wilt disease ,Plant culture ,SB1-1110 - Abstract
Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed by cultural as well as PCR analysis using species-specific primers. The roots of transgenic plants were wounded to facilitate the entry of the pathogen. The histopathological analysis revealed the presence of mycelium in vascular bundles. However, none of the plants showed symptoms of wilt disease during the investigation. In vitro pathogen inhibition assay and subsequently spore germination assay revealed that the crude leaf extract of transformed plants inhibited the germination of fungal conidia. The leaf tissue studied for expression of endochitinase revealed that two transgenic plants showed very high activity of N-acetyl-d-glucosamine (0.741 and 0.738 µM/min/µg of protein, respectively) which clearly indicated that transgenic plants could not develop any symptoms of wilt disease due to overexpression of endochitinase.
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- 2016
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6. Electrochemical Wearable Sensor for Biomedical and Healthcare Applications
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Yugender Goud Kotagiri, Shekher Kummar, Roger Narayan, Vinay Sharma, and Rupesh Kumar Mishra
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- 2022
7. COMPARATIVE STUDY OF DEXMEDETOMIDINE, TRAMADOL AND PETHIDINE FOR THE TREATMENT OF SHIVERING IN POST-NEURAXIAL ANAESTHESIA
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Chandeshwar Choudhary, Rajeev Krishan, Debarshi Jana, and Rupesh Kumar Mishra
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business.industry ,Pethidine ,03 medical and health sciences ,0302 clinical medicine ,030202 anesthesiology ,Anesthesia ,medicine ,Shivering ,030212 general & internal medicine ,Tramadol ,Dexmedetomidine ,medicine.symptom ,business ,medicine.drug - Abstract
Objective: This study was done for comparative study of dexmedetomidine with that o tramadol and pethidine in the treatment of post-neuraxial anaesthesia shivering. Design: This was a prospective, randomised, double-blinded study. Setting And Subjects: Hundred patients of both genders, aged 18–70 years with American Society of Anesthesiologists physical status I and II undergoing neuraxial a( spinal or combined spinal and epidural)anaesthesia for elective surgery were enrolled in this study. Sixty of them developed shivering after an intrathecal injection of 0.5% hyperbaric bupivacaine 15 mg. They were then randomly allocated to receive either intravenous dexmedetomidine 0.5 μg/kg, pethidine 0.5 mg/kg or tramadol 0.5 mg/kg. Outcome measures: The response rate to treatment, the degree of sedation and the side-effects were recorded. Results: The response rate to treatment was highest in the dexmedetomidine group, and it was only signicant when compared to tramadol group (p = 0.0012). It was noted that the response rate was higher in the pethidine than in the tramadol group. This difference was not statistically signicant (p = 0.082). The sedation score post treatment was similar in all three groups, but more patients in the dexmedetomidine group developed hypotension and bradycardia (p< 0.05). Conclusion: Dexmedetomidine 0.5 μg/ml was more effective than tramadol 0.5 mg/ml and pethidine 0.5 mg/ml, and both tramadol and pethidine were found to have similar efcacy, in the treatment of post-neuraxial anaesthesia shivering. However, dexmedetomidine caused a higher incidence of hypotension and bradycardia.
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- 2021
8. Electronic-free and low-cost wireless sensor tag for monitoring fish freshness
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Sarath Gopalakrishnan, Sina Nejati, Sotoudeh Sedaghat, Krish Gupta, Rupesh Kumar Mishra, and Rahim Rahimi
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Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
9. Printed graphene-based electrochemical sensor with integrated paper microfluidics for rapid lidocaine detection in blood
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Akshay Krishnakumar, Rupesh Kumar Mishra, Sachin Kadian, Amin Zareei, Ulisses Heredia Rivera, and Rahim Rahimi
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Microfluidics ,Environmental Chemistry ,Humans ,Lidocaine ,Pain ,Graphite ,Biosensing Techniques ,Electrochemical Techniques ,Biochemistry ,Electrodes ,Spectroscopy ,Analytical Chemistry - Abstract
Topical lidocaine patches are commonly used to relieve pain and suffering in various clinical and household settings. Despite its extensive use, excessive skin absorption during numbing or pain reduction procedures can cause systemic toxicity, which can lead to life-threatening conditions. Rapid and reliable monitoring of escalating levels of lidocaine in the blood could help management/prevention of lidocaine overdose and its associated complications. To address this need, here we have developed a disposable point-of-care (POC) diagnostic platform composed of an integrated graphene-based electrochemical sensor with paper-based microfluidics for rapid detection of lidocaine levels in serum and blood samples. The fabrication process takes advantage of advanced, scalable manufacturing techniques, including printing, laser processing, and nondestructive near infrared (NIR) drying. The sensitivity tests of the platform revealed a sensitivity of ∼0.2 μA μM
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- 2022
10. Electrochemical sensor for rapid detection of fentanyl using laser-induced porous carbon-electrodes
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Rupesh Kumar, Mishra, Akshay, Krishnakumar, Amin, Zareei, Ulisses, Heredia-Rivera, and Rahim, Rahimi
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Fentanyl ,Lasers ,Reproducibility of Results ,Electrodes ,Porosity ,Carbon ,Analytical Chemistry - Abstract
The growing pervasiveness of opioid-based drugs such as fentanyl and its analogs represent a foremost hazard to the civilian population and burden on the first responders and clinicians. Thus, to enable a rapid and low-cost surveillance system to detect fentanyl in a non-ideal environment, we demonstrate the use of laser-induced nano-porous carbon structures directly onto commercially available polyimide sheets for rapid and cost-effective manufacturing of electrochemical sensors for fentanyl detection. The porous carbon surface instigated by various laser energy densities was analyzed towards morphological, vibrational, and fentanyl sensing properties. The results showed that laser carbonized electrode (LCE) prepared with 31 J/cm
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- 2022
11. Knowledge and Compliance of Iron and Folic Acid Supplementation among Pregnant & Postnatal Women in a Hospital of Kathmandu
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Kalpana Tiwari and Rupesh Kumar Mishra
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Compliance (physiology) ,medicine.medical_specialty ,business.industry ,Obstetrics ,Medicine ,business ,Folic acid supplementation - Abstract
The high prevalence of iron deficiency anemia and low compliance of iron and folic acid supplementation was reported among pregnant women in Nepal. This study aimed to determine the knowledge and compliance of iron and folic acid supplementation among pregnant and postnatal women in Kathmandu. A descriptive cross-sectional study design was adopted for the study. Sample size was 103 and purposive sampling technique was used to collect the data. All Pregnant women of having above 7 month pregnancy and postnatal’s up to 6 months women were included. Data was collected by using self-administered questionnaire .All the obtained data were analyzed with the help of SPSS version 20. Assessment of knowledge revealed that 85.6 % (88) of the participants have adequate knowledge regarding iron and folic acid supplementation .The findings showed 78.6% (81) of the respondents had iron and folic acid compliance as standard of Nepal government that is to be taken minimum 180 tablet before delivery. It concludes that the level of knowledge and compliance rate of iron and folic acid supplementation in Kathmandu is satisfactory in comparisons to national status i.e. 42% according to Nepal Demographic health survey 2016. Pearson chi-square test shows that level of knowledge on iron and folic acid tablet is not associated with the compliance of iron and folic acid tablet (χ²= 0.019, p< 0.889). The main reason of respondents having compliance is due to having proper counseling from health worker and non compliance is due to forgetting to have tablet. Therefore improving knowledge level only can’t improve compliance level, various factors like counseling, accessibility and availability of iron and folic acid tablet, side effect of tablet and family support also affects the compliance of iron and folic acid supplementation.
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- 2020
12. Electronic-Free Low-Cost Wireless Sensor Tag for Monitoring Fish Freshness
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Sarath Gopalakrishnan, Sina Nejati, Sotoudeh Sedaghat, Krish Gupta, Rupesh Kumar Mishra, and Rahim Rahimi
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
13. Food Safety Analysis Using Electrochemical Biosensors
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Geetesh Kumar Mishra, Abbas Barfidokht, Farshad Tehrani, and Rupesh Kumar Mishra
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food safety ,biosensors ,electrochemical technique ,food analysis ,Chemical technology ,TP1-1185 - Abstract
Rapid and precise analytical tools are essential for monitoring food safety and screening of any undesirable contaminants, allergens, or pathogens, which may cause significant health risks upon consumption. Substantial developments in analytical techniques have empowered the analyses and quantitation of these contaminants. However, conventional techniques are limited by delayed analysis times, expensive and laborious sample preparation, and the necessity for highly-trained workers. Therefore, prompt advances in electrochemical biosensors have supported significant gains in quantitative detection and screening of food contaminants and showed incredible potential as a means of defying such limitations. Apart from indicating high specificity towards the target analytes, these biosensors have also addressed the challenge of food industry by providing high analytical accuracy within complex food matrices. Here, we discuss some of the recent advances in this area and analyze the role and contributions made by electrochemical biosensors in the food industry. This article also reviews the key challenges we believe biosensors need to overcome to become the industry standard.
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- 2018
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14. Identification of Potential Vaccine Candidates Against SARS-CoV-2 to Fight COVID-19: Reverse Vaccinology Approach (Preprint)
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Ekta Gupta, Rupesh Kumar Mishra, and Ravi Ranjan Kumar Niraj
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BACKGROUND The recent emergence of COVID-19 has caused an immense global public health crisis. The etiological agent of COVID-19 is the novel coronavirus SARS-CoV-2. More research in the field of developing effective vaccines against this emergent viral disease is indeed a need of the hour. OBJECTIVE The aim of this study was to identify effective vaccine candidates that can offer a new milestone in the battle against COVID-19. METHODS We used a reverse vaccinology approach to explore the SARS-CoV-2 genome among strains prominent in India. Epitopes were predicted and then molecular docking and simulation were used to verify the molecular interaction of the candidate antigenic peptide with corresponding amino acid residues of the host protein. RESULTS A promising antigenic peptide, GVYFASTEK, from the surface glycoprotein of SARS-CoV-2 (protein accession number QIA98583.1) was predicted to interact with the human major histocompatibility complex (MHC) class I human leukocyte antigen (HLA)-A*11-01 allele, showing up to 90% conservancy and a high antigenicity value. After vigorous analysis, this peptide was predicted to be a suitable epitope capable of inducing a strong cell-mediated immune response against SARS-CoV-2. CONCLUSIONS These results could facilitate selecting SARS-CoV-2 epitopes for vaccine production pipelines in the immediate future. This novel research will certainly pave the way for a fast, reliable, and effective platform to provide a timely countermeasure against this dangerous virus responsible for the COVID-19 pandemic.
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- 2021
15. Identification of Potential Vaccine Candidates Against SARS-CoV-2 to Fight COVID-19: Reverse Vaccinology Approach
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Ekta Gupta, Rupesh Kumar Mishra, and Ravi Ranjan Kumar Niraj
- Abstract
Background The recent emergence of COVID-19 has caused an immense global public health crisis. The etiological agent of COVID-19 is the novel coronavirus SARS-CoV-2. More research in the field of developing effective vaccines against this emergent viral disease is indeed a need of the hour. Objective The aim of this study was to identify effective vaccine candidates that can offer a new milestone in the battle against COVID-19. Methods We used a reverse vaccinology approach to explore the SARS-CoV-2 genome among strains prominent in India. Epitopes were predicted and then molecular docking and simulation were used to verify the molecular interaction of the candidate antigenic peptide with corresponding amino acid residues of the host protein. Results A promising antigenic peptide, GVYFASTEK, from the surface glycoprotein of SARS-CoV-2 (protein accession number QIA98583.1) was predicted to interact with the human major histocompatibility complex (MHC) class I human leukocyte antigen (HLA)-A*11-01 allele, showing up to 90% conservancy and a high antigenicity value. After vigorous analysis, this peptide was predicted to be a suitable epitope capable of inducing a strong cell-mediated immune response against SARS-CoV-2. Conclusions These results could facilitate selecting SARS-CoV-2 epitopes for vaccine production pipelines in the immediate future. This novel research will certainly pave the way for a fast, reliable, and effective platform to provide a timely countermeasure against this dangerous virus responsible for the COVID-19 pandemic.
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- 2022
16. Efficacy of Fungicides for Control of White Mold (Sclerotinia sclerotiorum Lib.) De Bary in Lima Bean
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Ashutosh Pandey, Amita J Mathew, Madhu Kamle, Rupesh Kumar Mishra, and Pradeep Kumar
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Sclerotinia sclerotiorum ,White Mold ,Phaseolous lunatus ,Lima Bean ,Fungicides ,Plant culture ,SB1-1110 - Abstract
White mold of lima bean (Phaseolous lunatus) caused by Sclerotinia sclerotiorum is a major disease in India. Isolates of the pathogen from different region of Uttar Pradesh were assayed both in vitro and in the greenhouse (in vivo) for their sensitivity to eight commercially available fungicides, viz., dithiocarbamic acid, carbendazim, ziram, phenylthiourea, carboxin + dithiocarbamic acid, difenoconazole, hydrogen sulphide, and mancozeb. Phenylthiourea and difenoconazole were found to be most effective and these inhibited radial growth of the test organism a level of to 71.5% and 70.5%, respectively. These two fungicides were also found as most promising in controlling the disease under greenhouse conditions, reducing disease severity to 0.14% and 0.22%, respectively compared to the control where it was 18.9%.
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- 2012
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17. Runoff Prediction Using Artificial Neural Networks
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Varnica Kapoor, Prachi Arya, and Rupesh Kumar Mishra
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Artificial Neural Network, Rainfall Runoff, Neurons - Abstract
The concept of rainfall-runoff variation is a non-linear event and highly tedious and continuously changing process. It involves different parameters which include i.e. mainly rainfall, soil, morphology and vegetation. The modelling for runoff prediction requires many engineering applications. The biggest obstacles in prediction of runoff are the estimation of extreme values. Unless models are not able to get the dynamics of rainfall-runoff process accurately, accurate prediction of these extremes is not possible. Various approaches have been adopted to represent rainfall-runoff process. The best among those approaches is using Artificial Neural Network techniques for the development of long -term and short-term forecasting models. Comparision is held between various approaches for runoff forecasting using ANNs., {"references":["Singh, P. V., Kumar Akhilesh, Rawat J. S., & Kumar Devendra, Artificial Neural Networks based daily rainfall-runoff model for an agricultural hilly watershed, International Journal of Engineering and Management Sciences","Christian W. Dawson & Robert Wilby, An Artificial Neural Network approach to rainfall-runoff modelling, Hydrological Sciences Journal","Satanand Mishra, Prince Gupta, S. K. Pandey & J. P. Shukla, An Efficient approach of Artificial Neural Network in runoff forecasting, International Journal of Computer Applications","Yonas B. Dibike & Dimitri P. Solomatine, Riverflow forecasting using Artificial Neural Networks, EGS Journal of Physics and Chemistry of the Earth","Gallant, A. R., and H. White, On learning the derivatives of an unknown mapping with multilayer feedforward networks, Neural Networks, 5, 129-138, 1992","Yojna Arora, Abhishek Singhal, Abhay Bansal, A Study of Applications of RBF Network, International Journal of Computer Applications","Yogesh Shirke, Prof. Dr. Rameshwar Kawitkar, Mr. Selva , Artificial Neural Network based Runoff Prediction Model for a Reservoir,International Journal of Engineering Research & Technology (IJERT)"]}
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- 2019
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18. Editorial: Patents on Decontamination of Heavy Metals from Environment
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Vinay Sharma and Rupesh Kumar Mishra
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Materials science ,Waste management ,010405 organic chemistry ,Bioengineering ,Heavy metals ,Nanotechnology ,Human decontamination ,010402 general chemistry ,01 natural sciences ,Applied Microbiology and Biotechnology ,0104 chemical sciences ,Biotechnology - Published
- 2017
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