Search

Major earthquakes cause serious damage to ecosystem structure and function through their huge destructive force and subsequent geohazards. Understanding the characteristics of post-earthquake land use change is of great interest to assess the effects of ecological restoration in earthquake-affected areas. However, little is known about the consequences of land use change on a small scale due to incomplete, non-comprehensive, and sparse research data. Here, we used remote-sensing images to study the land use change characteristics of the Longxi River before and after the Wenchuan earthquake by calculating the land use dynamics degree, transition matrix, and gravity center of the Longxi River Basin from 2005 to 2015. The Wenchuan earthquake disaster did not affect the main dominance of forests. Grassland, the second dominant land type, was replaced by geohazard-induced unutilized land after the earthquake. Compared with pre-earthquake in 2005, the areas of cultivated land, forest, grassland, and water area decreased, while those of construction land and unutilized land increased in 2015. The single land use dynamic degree and spatial dynamic change degree show the highest transition intensity of other land use types to unutilized land between 2005 and 2009. However, the degrees of all land use types decreased between 2009 and 2015. Both banks of the lower reach of the Longxi River had high and developing comprehensive indexes of land use degrees during the study period. The gravity centers of grassland, construction land, water, and cultivated land changed from north to south, while forest and unutilized land had the opposite pattern. Our results provide useful information for ecological restoration, ecological security, and soil-erosion control in earthquake-affected areas.

A nucleotide metabolic enzyme restricts viral lytic replication via protein deamidation., Protein nuclear translocation is highly regulated and crucial for diverse biological processes. However, our understanding concerning protein nuclear import is incomplete. Here we report that a cellular purine synthesis enzyme inhibits protein nuclear import via deamidation. Employing human Kaposi’s sarcoma-associated herpesvirus (KSHV) to probe the role of protein deamidation, we identified a purine synthesis enzyme, phosphoribosylformylglycinamidine synthetase (PFAS) that inhibits KSHV transcriptional activation. PFAS deamidates the replication transactivator (RTA), a transcription factor crucial for KSHV lytic replication. Mechanistically, deamidation of two asparagines flanking a positively charged nuclear localization signal impaired the binding of RTA to an importin β subunit, thus diminishing RTA nuclear localization and transcriptional activation. Finally, RTA proteins of all gamma herpesviruses appear to be regulated by PFAS-mediated deamidation. These findings uncover an unexpected function of a metabolic enzyme in restricting viral replication and a key role of deamidation in regulating protein nuclear import.

Kaposi’s sarcoma-associated herpesvirus (KSHV) has latent and lytic replication phases, both of which contribute to the development of KSHV-induced malignancies. Among the numerous factors identified to regulate the KSHV life cycle, oxidative stress, caused by imbalanced clearing and production of reactive oxygen species (ROS), has been shown to robustly disrupt KSHV latency and induce viral lytic replication. In this study, we identified an important role of the antioxidant defense factor forkhead box protein O1 (FoxO1) in the KSHV life cycle. Either chemical inhibition of the FoxO1 function or knockdown of FoxO1 expression led to an increase in the intracellular ROS level that was subsequently sufficient to disrupt KSHV latency and induce viral lytic reactivation. On the other hand, treatment with N-acetyl-l-cysteine (NAC), an oxygen free radical scavenger, led to a reduction in the FoxO1 inhibition-induced ROS level and, ultimately, the attenuation of KSHV lytic reactivation. These findings reveal that FoxO1 plays a critical role in keeping KSHV latency in check by maintaining the intracellular redox balance. IMPORTANCE Kaposi’s sarcoma-associated herpesvirus (KSHV) is associated with several cancers, including Kaposi’s sarcoma (KS). Both the KSHV latent and lytic replication phases are important for the development of KS. Identification of factors regulating the KSHV latent phase-to-lytic phase switch can provide insights into the pathogenesis of KSHV-induced malignancies. In this study, we show that the antioxidant defense factor forkhead box protein O1 (FoxO1) maintains KSHV latency by suppressing viral lytic replication. Inhibition of FoxO1 disrupts KSHV latency and induces viral lytic replication by increasing the intracellular ROS level. Significantly, treatment with an oxygen free radical scavenger, N-acetyl-l-cysteine (NAC), attenuated the FoxO1 inhibition-induced intracellular ROS level and KSHV lytic replication. Our works reveal a critical role of FoxO1 in suppressing KSHV lytic replication, which could be targeted for antiviral therapy.

Cell proliferation and inflammation are two metabolically demanding biological processes. How these competing processes are selectively executed in the same cell remains unknown. Here, we report that the enzyme carbamoyl-phosphate synthetase, aspartyl transcarbamoylase, and dihydroorotase (CAD) deamidates the RelA subunit of NF-κB in cancer cells to promote aerobic glycolysis and fuel cell proliferation in tumorigenesis. This post-translational modification switches RelA function from mediating the expression of NF-κB-responsive genes to that of glycolytic enzymes, thus shunting the cell's inflammatory response to aerobic glycolysis. Further, we profiled diverse human cancer cell lines and found that high CAD expression and a subset of RELA mutations correlated with RelA deamidation. And by use of inhibitors of key glycolytic enzymes, we validated the pivotal role of RelA deamidation in tumorigenesis of cancer cell lines. This work illuminates a mechanism by which protein deamidation selectively specifies gene expression and consequent biological processes.

In interest point based human action recognition, local descriptors are used to represent information in the neighbourhood around each extracted space-time interest point. The performance of the action recognition systems highly depends on the invariance and distinctiveness of the local spatiotemporal descriptor adopted. In this paper, we propose a new descriptor based on the Wavelet Transform taking advantage of its capability in compacting and discriminating data. We evaluate this descriptor on the extensively studied KTH action dataset, using the Bag-of Features framework. Results show the Wavelet Transform based descriptor achieves the recognition rate of 93.89%, which is better than most of the state-of-the-art methods.

We propose an algorithm for synthesizing natural images consisting of composite textures in this chapter. Existing texture synthesis techniques usually fail to preserve the structural layout of the input image. To overcome this drawback, a target image which contains the layout information of the input is used to control the synthesis process. With the guidance of this target image, the synthesized output resembles the input globally and is composed of the original textures. For images composed of textured background and a non-textured foreground object, segmentation is first applied on the main object and texture synthesis is used on the background texture. In comparison to other synthesis methods, the proposed solution yields significantly better results for natural images.