148 results on '"Ruolan Li"'
Search Results
2. Economic benefit of ecological remediation of mercury pollution in southwest China 2007–2022
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Huifang Jiang, Junyao Yan, Ruolan Li, Shaochen Yang, Guopei Huang, Wenjuan Wang, Yanxu Zhang, Ping Li, and Xinbin Feng
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Ecological remediation ,Methylmercury ,Rice consumption ,Health risk ,Economic benefits ,Environmental sciences ,GE1-350 - Abstract
Methylmercury (MeHg) exposure via rice consumption poses health risk to residents in mercury contaminated areas, such as the Wanshan Hg mining area (WSMA) in southwest China. Making use of the published data for WSMA, this study developed a database of rice MeHg concentrations for different villages in this region for the years of 2007, 2012, 2017, and 2019. The temporal changes of human MeHg exposure, health effects, and economic benefits under different ecological remediation measures were then assessed. Results from this study revealed a decrease of 3.88 μg/kg in rice MeHg concentration and a corresponding reduction of 0.039 μg/kg/d in probable daily intake of MeHg in 2019 compared to 2007 on regional average in the WSMA. Ecological remediation measures in this region resulted in the accumulated economic benefits of $38.7 million during 2007–2022, of which 84 % was from pollution source treatment and 16 % from planting structure adjustment. However, a flooding event in 2016 led to an economic loss of $2.43 million (0.38 % of regional total Gross Domestic Product). Planting structure adjustment generates the greatest economic benefits in the short term, whereas pollution source treatment maximizes economic benefits in the long term and prevents the perturbations from flooding event. These findings demonstrate the importance of ecological remediation measures in Hg polluted areas and provide the foundation for risk assessment of human MeHg exposure via rice consumption.
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- 2024
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3. Zuojin Pill inhibits the proliferation of esophageal cancer cells by inducing ROS production and apoptosis via regulation of PI3K/Akt signalling
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Lingyu Wang, Ruolan Li, Meiyan Li, Jiayi Sun, Jia Liu, Min Xie, Yunhui Chen, and Songqi Tang
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Zuojin Pill ,Esophageal cancer ,ROS production ,apoptosis ,PI3K/Akt ,Science (General) ,Q1-390 - Abstract
This study aimed to investigate the inhibitory effect of Zuojin Pill (ZJP) on KYSE 150 cells and xenograft tumours of the digestive tract cancer – esophageal cancer (EC) and its underlying mechanism. Initially, in vivo research demonstrated that oral administration of ZJP effectively inhibited the growth of EC tumours, and in vitro studies revealed that ZJP suppressed the proliferation, migration, and invasion of cells. Then, network pharmacology prediction identified the core target, active ingredient, and involvement of the PI3K-Akt signalling pathway in ZJP treatment of EC. Cell studies validated that ZJP affected the expression of core targets, and its active ingredients inhibited the proliferation of cells. Additionally, ZJP was shown to induce cell apoptosis, decrease mitochondrial membrane potential, and increase the level of intracellular oxidative stress. Further experimental verifications suggested that the inhibitory effects of ZJP on EC might be attributed to the modulation of PI3 K/Akt signalling pathways.
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- 2024
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4. Constructing a Transient Ischemia Attack Model Utilizing Flexible Spatial Targeting Photothrombosis with Real-Time Blood Flow Imaging Feedback
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Xuan Zhu, Zichao Yi, Ruolan Li, Chen Wang, Wenting Zhu, Minghui Ma, Jinling Lu, and Pengcheng Li
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transient ischemic attack ,real-time blood flow imaging ,spatial targeting photothrombosis ,distal middle cerebral artery ,thrombus ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Transient ischemic attack (TIA) is an early warning sign of stroke and death, necessitating suitable animal models due to the associated clinical diagnostic challenges. In this study, we developed a TIA model using flexible spatially targeted photothrombosis combined with real-time blood flow imaging feedback. By modulating the excitation light using wavefront technology, we precisely created a square light spot (50 × 250 µm), targeted at the distal middle cerebral artery (dMCA). The use of laser speckle contrast imaging (LSCI) provided real-time feedback on the ischemia, while the excitation light was ceased upon reaching complete occlusion. Our results demonstrated that the photothrombus formed in the dMCA and spontaneously recanalized within 10 min (416.8 ± 96.4 s), with no sensorimotor deficits or infarction 24 h post-TIA. During the acute phase, ischemic spreading depression occurred in the ipsilateral dorsal cortex, leading to more severe ischemia and collateral circulation establishment synchronized with the onset of dMCA narrowing. Post-reperfusion, the thrombi were primarily in the sensorimotor and visual cortex, disappearing within 24 h. The blood flow changes in the dMCA were more indicative of cortical ischemic conditions than diameter changes. Our method successfully establishes a photochemical TIA model based on the dMCA, allowing for the dynamic observation and control of thrombus formation and recanalization and enabling real-time monitoring of the impacts on cerebral blood flow during the acute phase of TIA.
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- 2024
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5. Structural trade-off regulation of composite aerogels via 'island-bridge' design for advanced nickel-iron batteries
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Tieqi Huang, Wenjing Liu, Yi Liu, Qianqian Hou, Suyue Chen, Ruolan Li, and Hongtao Liu
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Composite aerogels ,Graphene oxide ,Nickel-iron batteries ,Three-dimensional frameworks ,Trade-off regulation ,Energy industries. Energy policy. Fuel trade ,HD9502-9502.5 ,Renewable energy sources ,TJ807-830 - Abstract
Nickel-iron batteries (NIBs) as typical aqueous batteries have ideal electrochemical performance in theory, making them potential candidates for power supply in specific conditions. However, they still face numerous thorny practical problems, including unsatisfactory durability and undesirable lifespan of anodes. Here, we put forward a new “island-bridge” design for building up rational anode networks by architecting active nanoparticle “islands” and conductive nanosheet “bridges” to realize trade-off strategy in electrochemistry. In comparison with normally-designed metal-graphene composites which are composed of dominate graphene substrates with metal-based additives, “island-bridge” design leads to optimized balance of abundant redox sites and enough conductive connections. As a result, the aerogels stemmed from “island-bridge” design display outstanding anode performance for NIBs with large capacity, high rate capability and long lifespan, which are far more superior than graphene-dominated composite anodes and neat metal oxides anodes. Additionally, theoretical calculations validate the mechanism of advantageous “island-bridge” design that it endows the anodes with numerous active sites, high-speed electron pathways and suitable ion-penetrable nanosized-pores. This work paves a way to take insights into micro-nano structured composite electrodes, providing a feasible way to prepare high-performance anodes for practical aqueous energy storage.
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- 2024
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6. A mulberry 9-cis-epoxycarotenoid dioxygenase gene MaNCED1 is involved in plant growth regulation and confers salt and drought tolerance in transgenic tobacco
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Panpan Zhu, Ruolan Li, Wei Fan, Zhongqiang Xia, Jun Li, Chuanhong Wang, and Aichun Zhao
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mulberry NCED ,plant hormones ,abiotic stress ,seed germination ,root elongation ,Plant culture ,SB1-1110 - Abstract
The phytohormone abscisic acid (ABA) is vital in regulating root elongation, seed germination, and abiotic stress responses in plants. Conversely, the mechanisms of ABA in mulberry root growth, seed germination, and abiotic stress responses are poorly understood. Here, we reported that exogenous ABA and drought treatment inhibited the growth of mulberry seedlings but significantly increased the ratio of root/stem. Inhibition of ABA synthesis by fluridone and sodium tungstate resulted in the decrease of root/stem ratio. We also showed that the expression of MaNCED1 in the root was strongly induced by drought and salt stress. Increasing the expression of MaNCED1 in tobacco using overexpression leads to increased root elongation and reduced seed germination. Compared with the wild type, the accumulation of H2O2 and MDA was reduced, while the POD activity and proline content was increased in the transgenic plants after drought and salt treatment. Further studies revealed increased resistance to drought and salt stress in MaNCED1 overexpressed tobaccos. Meanwhile, the auxin and ethylene signal pathway-related gene expression levels increased in MaNCED1 overexpressed tobaccos. This study demonstrated the roles of mulberry MaNCED1 in regulating plant development and abiotic stress responses. It gave further insights into the coordinated regulation of ABA, auxin, and ethylene in seed growth and germination.
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- 2023
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7. Attenuating lipid metabolism in atherosclerosis: The potential role of Anti-oxidative effects on low-density lipoprotein of herbal medicines
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Huxinyue Duan, Pan Song, Ruolan Li, Hong Su, and Lisha He
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atherosclerosis ,oxidative stress ,lipid metabolism ,low-density lipoprotein ,herbal medicines ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Atherosclerosis (AS) is a multifactorial chronic disease with great harm to the health of human being, which is a basic pathogenesis of many cardiovascular diseases and ultimately threatens human life. Abnormal blood lipid level is one of the most common diagnostic indicators of AS in clinic, and lipid metabolism disorder is often observed in patients with AS. Cholesterol is an important lipid in the human body, which is of great significance for maintaining normal life activities. Generally, cholesterol is transported to peripheral tissues by low-density lipoprotein (LDL), and then transported to the liver by high-density lipoprotein (HDL) via its cholesterol reverse transport function, and finally discharged. Under oxidative stress condition, LDL is commonly oxidized to the form ox-LDL, which is ingested by macrophages in large quantities and further forms foam cells, disrupting the normal metabolic process of cholesterol. Importantly, the foam cells are involved in forming atherosclerotic plaques, whose rupture may lead to ischemic heart disease or stroke. Furthermore, ox-LDL could also promote the development of AS by damaging vascular endothelium, promoting the migration and proliferation of smooth muscle cells, and activating platelets. Therefore, inhibiting LDL oxidation may be an effective way to improve lipid metabolism and prevent AS. In recent years, increasing studies have shown that herbal medicines have great potentiality in inhibiting LDL oxidation and reducing ox-LDL induced foam cell formation. Accordingly, this paper summarized current research on the inhibitory effects of herbal medicines against LDL oxidation and foam cell formation, and made a brief description of the role of cholesterol and LDL in lipid metabolism disorder and AS pathogenesis. Importantly, it is suggested that herbal medicines could inhibit LDL oxidation and regulate cholesterol homeostasis via downregulation of CD36 and SR-A, whereas upregulation of ABCA1 and ABCG1.
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- 2023
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8. Modeling and simulation of extended ant colony labor division for benefit distribution of the all-for-one tourism supply chain with front and back decoupling
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Rundong Liao, Shufang Li, Conghui Wu, Xiang Zhang, Changbing Jiang, and Ruolan Li
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all-for-one tourism ,division of labor in ants ,front-background decoupling ,extended ant colony labor division model ,benefit distribution ,Biotechnology ,TP248.13-248.65 - Abstract
This paper takes the supply chain alliance under the decoupling of the front and back of the all-for-one tourism as the research object. Considering the three behavior stimuli of self-benefit, altruism, and invariance, this article resets the attributes such as environmental stimuli and response threshold of ants based on the characteristics of the all-for-one tourism supply chain with shared services as the core under the decoupling of the front and back. Moreover, it introduces dual intervention factors to coordinate the benefit distribution process of different member companies, takes fairness as the main goal of benefit distribution, introduces relative deprivation as the measure index of fairness, and establishes a dynamic all-for-one tourism supply chain alliance benefit distribution model. The experimental results show that the extended model has good flexibility of benefit distribution and realizes the fair distribution of supply chain benefits.
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- 2023
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9. Pharmacokinetics, tissue distribution, and antitumor activity of a novel compound, NY-2, in non-small cell lung cancer
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Yingshi Zhang, Chang Xu, Xiangbo Xu, Lingxiang Ma, Ruolan Li, Zihua Xu, and Qingchun Zhao
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NY-2 ,pharmacokinetics ,tissue distribution ,antitumor activity ,non-small cell lung cancer ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Introduction: ZLDI-8, which has a relatively strong antitumor activity, is an inhibitor of ADAM-17 and acts on the Notch signaling pathway. To further optimize its structure and improve its activity, a series of derivatives of ZLDI-8 was synthesized. NY-2 was the most effective derivative based on preliminary activity screening in vitro, with no obvious toxicity after administration in vivo.Method: The study aimed to determine the pharmacokinetics, tissue distribution, hepatotoxicity, nephrotoxicity, and antitumor activity of compound NY-2 on non-small cell lung cancer (NSCLC) in vitro and in vivo.Results: The in vivo pharmacokinetics parameters of NY-2 were better than those of ZLDI-8. The tissue distribution analysis showed that tail vein injection of 6 mg/kg of NY-2 in rats resulted in the highest concentration in the lung, so we hypothesized that NY-2 might be effective in the treatment of non-small cell lung cancer. In vitro assays showed that NY-2 significantly inhibited tumor colony formation, invasion, and migration and increased LDH activity and apoptosis in a concentration-dependent manner in non-small cell lung cancer cells. NY-2 also inhibited the formation of lung metastases without significant toxicity to major organs in nude mice.Conclusion: Compared with the parent compound, ZLDI-8, the activity and safety of NY-2 were higher. NY-2 acts on ADAM17 and simultaneously affects the downstream Notch1 and integrinβ1 signaling pathways resulting in antitumor activity. Thus, NY-2 could be a potential antitumor agent, inhibiting the organization and development of non-small cell lung cancer.
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- 2023
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10. EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels
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Qingqing Gao, Xia Li, Chunfan Xiang, Ruolan Li, Hongchun Xie, Jia Liu, Xiaoning Li, Guanghui Zhang, Shengchao Yang, Yanli Liang, Chenxi Zhai, and Yan Zhao
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EbbHLH80 ,salt tolerance ,flavonoids ,ROS ,stress-responsive genes ,Erigeron breviscapus ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
bHLH transcription factors are involved in multiple aspects of plant biology, such as the response to abiotic stress. Erigeron breviscapus is a composite plant, and its rich flavonoids have strong preventive and therapeutic effects on cardio cerebral vascular disease. EbbHLH80, a gene from E. breviscapus that positively regulates flavonoid synthesis, was previously characterized. However, it is unclear whether EbbHLH80 increases flavonoid accumulation, which affects salt tolerance. The function of EbbHLH80 in transgenic tobacco seeds was identified by phylogenetic analysis and metabolome-transcriptome analysis. We investigated the role of EbbHLH80 in salt stress response. Our results showed that the expression of EbbHLH80 increased following salt treatment. Integrating the metabolome and transcriptome analysis of EbbHLH80-OE and Yunyan 87 (WT) seeds, we identified several genes and metabolites related to flavonoid biosynthesis and salt stress. Moreover, EbbHLH80-OE plants displayed higher salt tolerance than wild-type plants during seed germination and seedling growth. After salt treatment, transgenic tobacco had significantly lower levels of reactive oxygen species (ROS) than WT, with enhanced levels of antioxidant enzyme expression. Altogether, our results demonstrated that EbbHLH80 might be a positive regulator, promoting salt tolerance by modulating ROS scavenging and increasing stress-responsive genes.
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- 2023
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11. Pyracantha fortuneana (Maxim.) Li: A comprehensive review of its phytochemistry, pharmacological properties, and product development
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Lingyu Wang, Ruolan Li, Qing Zhang, Jia Liu, Ting Tao, Ting Zhang, Chunjie Wu, Qiang Ren, Xufeng Pu, and Wei Peng
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Pyracantha fortuneana (Maxim.) Li ,phytochemistry ,botany ,pharmacological properties ,product development ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Pyracantha fortuneana (Maxim.) Li has been used as a herbal medicine in China in its long history. Since ancient times, the fruits of P. fortuneana has been considered a functional food to improve various diseases. Many bioactive substances, including proanthocyanidins, phenols, polysaccharides, and dietary fibers, have been isolated and identified from the P. fortuneana, which possess diverse biological properties both in vitro and in vivo. Although the researches on the P. fortuneana have achieved extensive progress, the systematic study of its biological activities is still relatively lacking. In addition, accumulating researches focus on the landscape value of the P. fortuneana and the development of its by-products. The by-products of P. fortuneana, which show good development potentials in the field of agricultural production and environmental protection, are important for improving the economic value of P. fortuneana and its significance. After extensive reviewing and analyzing the existing published articles, books, and patents, this study aims to a systematic and summarized research trends of P. fortuneana and its phytochemical compositions, nutritional values, pharmacological effects and health benefits of its extracts/monomers, which would be beneficial for the future development of this medicinal plant as functional food or drugs.
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- 2022
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12. Alpiniae oxyphyllae fructus possesses neuroprotective effects on H2O2 stimulated PC12 cells via regulation of the PI3K/Akt signaling Pathway
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Ruolan Li, Lingyu Wang, Qing Zhang, Huxinyue Duan, Die Qian, Fei Yang, and Jun Xia
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alzheimer’s disease ,Alpiniae oxyphyllae fructus ,network analysis ,apoptosis ,PI3K ,akt ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Backgroud: Alzheimer’s disease (AD) is a typical neurodegenerative disease, which occurs in the elderly population. Alpiniae oxyphyllae Fructus (AOF) is a traditional Chinese medicine that has potential therapeutic effect on AD, but the mechanism behind it is unclear.Methods: Firstly, the main chemical components of AOF were identified by LC-MS, while the main active ingredients and targets were screened by TCMSP database. At the same time, AD-related target proteins were obtained using Genecards and OMIM databases. PPI was constructed by cross-linking AOF and AD targets, and GO enrichment analysis and KEGG pathway enrichment analysis were performed to identify the relevant biological processes and signaling pathways. Finally, based on the H2O2-stimulated PC12 cell, flow cytometry, WB and immunofluorescence experiments were performed to verify the protective effect of AOF on AD.Results: We identified 38 active ingredients with 662 non-repetitive targets in AOF, of which 49 were potential therapeutic AD targets of AOF. According to the GO and KEGG analysis, these potential targets are mainly related to oxidative stress and apoptosis. The role of AOF in the treatment of AD is mainly related to the PI3K/AKT signaling pathway. Protocatechuic acid and nootkatone might be the main active ingredients of AOF. In subsequent experiments, the results of CCK-8 showed that AOF mitigated PC12 cell damage induced by H2O2. Kits, flow cytometry, and laser confocal microscopy indicated that AOF could decrease ROS and increase the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), while AOF could also increase mitochondrial membrane potential (MMP), thereby inhibiting apoptosis. Finally, immunofluorescence and WB results showed that AOF inhibited the expression of BAX and caspase-3 in PC12 cells, and promoted the expression of Bcl-2. At the same time, the phosphorylation levels of PI3K and Akt proteins were also significantly increased.Conclusion: This study suggests that AOF had the potential to treat AD by suppressing apoptosis induced by oxidative stress via the PI3K/Akt pathway.
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- 2022
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13. Comprehensive evaluation method of the poultry house indoor environment based on gray relation analysis and analytic hierarchy process
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Peng Wen, Lihua Li, Hao Xue, Yuchen Jia, Liai Gao, Ruolan Li, and Limin Huo
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environmental comprehensive evaluation ,the gray relation degree ,the analytic hierarchy process ,the poultry house ,the weight ,Animal culture ,SF1-1100 - Abstract
ABSTRACT: Due to the antagonism and synergy among environmental factors in the poultry house, the influence process becomes extremely complex. As a result, it is difficult to predict and evaluate the degree of such influence accurately. In this paper, we study the poultry house environment factor and its relationship with poultry production performance, using the gray relation analysis (GRA) to filtrate the main factors that influence the evaluation of the poultry house environment. Put forward using the gray relation degree (GRD) to improve the method for structuring the judgment matrix, and weights are more objective and reasonable. The evaluation index system and evaluation model are constructed through the analytic hierarchy process (AHP). It is expected that the comprehensive evaluation of the indoor environment status of the poultry house can guide the optimization of the environmental control in the poultry house and obtain better production indicators of the poultry. In this study, the experimental broiler house was enclosed in autumn. Because of the ventilation system, the indoor environment is still affected by the outdoor environment. The top 3 in the calculation of weights were outdoor environment (0.4315), indoor temperature (0.2384), and indoor air quality (0.1687), which were consistent with experience. From October 24 to 27, the environmental evaluation values of the experimental broiler house were {2.4367, 2.8149, 2.3857, 2.5669}, that is, the evaluation results were {good, good, good, good}; consistent with the expert manual judgment. The correctness and practicability of the proposed method were verified. This paper provides a scientific basis for environmental evaluation and environmental control in the poultry house.
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- 2022
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14. Emerging Roles of m6A RNA Methylation Regulators in Gynecological Cancer
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Wanjun Huang, Fanhua Kong, Ruolan Li, Xiang Chen, and Kunpeng Wang
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gynecological cancer ,N6-methyladenosine (m6A) ,epigenetics ,cervical cancer ,endometrial cancer ,ovarian cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Gynecological cancers seriously affect the reproductive system of females; diseases include ovarian tumors, uterine tumors, endometrial cancers, cervical cancers, and vulva and vaginal tumors. At present, the diagnosis methods of gynecological cancer are insufficiently sensitive and specific, leading to failure of early disease detection. N6-methyladenosine (m6A) plays various biological functions in RNA modification and is currently studied extensively. m6A modification controls the fate of transcripts and regulates RNA metabolism and biological processes through the interaction of m6A methyltransferase (“writer”) and demethylase (“erasers”) and the binding protein decoding m6A methylation (“readers”). In the field of epigenetics, m6A modification is a dynamic process of reversible regulation of target RNA through its regulatory factors. It plays an important role in many diseases, especially cancer. However, its role in gynecologic cancers has not been fully investigated. Thus, we review the regulatory mechanism, biological functions, and therapeutic prospects of m6A RNA methylation regulators in gynecological cancers.
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- 2022
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15. Efficacy and tolerability of Guizhi-Shaoyao-Zhimu decoction in gout patients: a systematic review and Meta-analysis
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Qing Zhang, Ruolan Li, Jia Liu, Wei Peng, Wenxiang Fan, Yongxiang Gao, Wei Jin, and Chunjie Wu
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safety evaluation ,traditional chinese medicine ,inflammatory arthritis ,heterogeneity ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Context Guizhi-Shaoyao-Zhimu decoction (GSZD), a famous ancient oriental Chinese prescription, has been widely used for thousands of years to treat ‘arthromyodynia’. Objective The clinical studies of GSZD for the treatment of gout were systematically reviewed to evaluate its clinical efficacy and safety. Methods All randomized controlled trials (RCTs) related to GSZD and gout were collected starting from the database establishment until 29 February 2020, from the Embase, PubMed, Cochrane Library, Web of Knowledge, VIP and other databases. This systematic review and meta-analysis were performed in strict accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) statement, and all analysis of the test was completed using Stata (SE12.0) and Revman (5.3). Results A total of 535 studies were searched, and 13 studies were included in our meta-analysis (n = 1056 participants). Compared with the conventional western medicine treatments, GSZD treatment yielded a significantly increase in the number of clinically effective patients (OR = 3.67, 95%CI = 2.39–5.64, p = 0.57), an improved mean reduction in the level of uric acid (MD = −54.06; 95% CI = −69.95 to −38.17). Meanwhile, the levels of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and interleukin-6 (IL-6) were also significantly decreased after the GSZD treatment with no increased relative risk of side-effects. Conclusions Our present works suggested that GSZD could be considered as an effective alternative remedy for clinical treatment of gout. In addition, it also provides a scientific basis for GSZD to be better applied in clinic in the future.
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- 2020
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16. Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head
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Dan Wang, Yicheng Liu, Dandan Tang, Shujun Wei, Jiayi Sun, Lvqiang Ruan, Lin He, Ruolan Li, Qiang Ren, Xiaoping Tian, and Yunhui Chen
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buxue tongluo pill ,osteonecrosis of the femoral head ,GEO database ,apoptosis ,PI3K/AKT ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The Buxue Tongluo pill (BTP) is a self-made pill with the functions of nourishing blood, promoting blood circulation, dredging collaterals, and relieving pain. It consists of Angelica sinensis (Oliv.) Diels, Pheretima aspergillum (E.Perrier), Panax notoginseng (Burk.) F. H. Chen, Astragalus membranaceus (Fisch.) Bge, and Glycyrrhiza uralensis Fisch. Various clinical practices have confirmed the therapeutic effect of BTP on osteonecrosis of the femoral head (ONFH), but little attention has been paid to the study of its bioactive ingredients and related mechanisms of action. In this study, UPLC/MS-MS combined with GEO data mining was used to construct a bioactive ingredient library of BTP and a differentially expressed gene (DEG) library for ONFH. Subsequently, Cytoscape (3.7.2) software was used to analyze the protein–protein interaction between BTP and DEGs of ONFH to screen the key targets, and functional annotation analysis and pathway enrichment analysis were carried out. Finally, 34 bioactive compounds were screened, which acted on 1,232 targets. A total of 178 DEGs were collected, and 17 key genes were obtained after two screenings. By bioinformatics annotation on these key genes, a total of 354 gene ontology (GO) functional annotation analyses and 42 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were obtained. The present study found that GO and KEGG enrichment were mainly related to apoptosis, suggesting that BTP may exert an anti-ONFH effect by promoting osteoclast apoptosis. Experiments in vitro demonstrated that BTP could increase the mitochondrial membrane potential (MMP) and induce remarkable apoptosis in osteoclasts. Furthermore, we determined the apoptosis marker of cleaved(C)-caspase-3, bcl-2, and bax and found that BTP could upregulate the C-caspase-3 and bax expression in osteoclasts and decrease the expression of bcl-2, p-Akt, and p-PI3K in a dose-dependent manner, indicating that BTP could induce PI3K/Akt-mediated apoptosis in osteoclasts to treat ONFH. This study explored the pharmacodynamic basis and mechanism of BTP against ONFH from the perspective of systemic pharmacology, laying a foundation for further elucidating the therapeutic effects of BTP against ONFH.
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- 2021
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17. An APSES Transcription Factor Xbp1 Is Required for Sclerotial Development, Appressoria Formation, and Pathogenicity in Ciboria shiraiana
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Shuai Zhang, Panpan Zhu, Boning Cao, Shuyu Ma, Ruolan Li, Xie Wang, and Aichun Zhao
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APSES transcription factor ,sclerotia ,pathogenicity ,host-induced gene silencing ,Ciboria shiraiana ,Microbiology ,QR1-502 - Abstract
Sclerotinia diseases are important plant fungal diseases that, causes huge economic worldwide losses every year. Ciboria shiraiana is the main pathogen that results in mulberry sclerotia diseases. Sclerotia and appressoria play important roles in long-term pathogen survival and in host infection during life and disease cycles. However, the molecular mechanisms of sclerotial development and appressoria formation in C. shiraiana have not been well studied. Here, an Asm1p, Phd1p, Sok2p, Efg1p and StuAp (APSES)-type transcription factor in C. shiraiana, CsXbp1, involved in sclerotial development and appressoria formation was functionally characterized. Bioinformatics analyses showed that CsXbp1 contained an APSES-type DNA binding domain. The expression levels of CsXbp1 were higher in sclerotia and during later stages of infection. Compared with wild-type strains, hyphal growth was slower, the number and weight of sclerotia were reduced significantly, and appressoria formation was obviously delayed in CsXbp1 RNA interference (RNAi) strains. Moreover, the CsXbp1 RNAi strains showed weakened pathogenicity owing to compound appressoria defects. Tobacco rattle virus-mediated host-induced gene silencing enabled Nicotiana benthamiana to increase its resistance to C. shiraiana by reducing the CsXbp1 transcripts level. Thus, CsXbp1 plays vital roles in sclerotial formation, appressoria formation, and pathogenicity in C. shiraiana. This study provides new insights into the infection mechanisms of C. shiraiana and plant resistance breeding.
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- 2021
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18. Impact of Climate Change on Food Security in Kazakhstan
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Danmeng Wang, Ruolan Li, Guoxi Gao, Nueryia Jiakula, Shynggys Toktarbek, Shilin Li, Ping Ma, and Yongzhong Feng
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climate change ,food security ,Kazakhstan ,yield of staple crops ,planting management measures ,Agriculture (General) ,S1-972 - Abstract
Global food production faces immense pressure, much of which can be attributed to climate change. A detailed evaluation of the impact of climate change on the yield of staple crops in Kazakhstan, a major food exporter, is required for more scientific planting management. In this study, the Mann–Kendall test and Theil–Sen Median slope were used to determine climate trends and staple food yields over the past 30 years; random forest was used to analyze the importance of monthly climatic factors; states were classified according to climatic factors through systematic clustering method; and lastly, the influence of climate on yield was analyzed using panel regression models. The upward trend in wind speed and potato yield throughout Kazakhstan was apparent. Furthermore, barley and wheat yields had increased in the southeast. We determined that for wheat, frostbite should be prevented after the warmer winters in the high-latitude areas. Except for July–August in the low-latitude areas, irrigation water should be provided in the other growth periods and regions. As similar effects were reported for barley, the same preventive measures would apply. For potatoes, tuber rot, caused by frost or excessive precipitation in May, should be prevented in high-latitude areas; soil dryness should be alleviated during the germination and seedling stages in low-latitude areas; and irrigation and cooling should be maintained during tuber formation and maturation. Furthermore, hot dry air in March and April could damage the crops.
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- 2022
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19. Alpinia oxyphylla oil induces apoptosis of hepatocellular carcinoma cells via PI3K/Akt pathway in vitro and in vivo
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Fuhai Hui, Xiaochun Qin, Qiao Zhang, Ruolan Li, Mingyue Liu, Tianshu Ren, Mingyi Zhao, and Qingchun Zhao
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Alpinia oxyplylla ,Petroleum ether fraction ,Hepatocellular carcinoma ,Apoptosis ,PI3K/Akt pathway ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: The anti-tumor properties of Alpinia oxyphylla Miquel (A. oxyphylla) extracts and their petroleum ether (PE) fractions have long attracted scientific attention. These extracts’ anti-tumor activity and mechanisms in vivo are still unclear. This study was designed to investigate the anti-tumor activity and the underlying mechanism of PE’s effect on hepatocellular carcinoma (HCC) in vitro and in vivo. Materials and method: The anti-tumor activity of PE was evaluated by MTT assay and xenograft study. Mechanistic studies of PE were analyzed by Hoechst 33342 staining, Annexin V-FITC/PI double-staining assay, immunohistochemical staining and western blot assay. The toxicity of the PE treatment was verified by the levels of liver and kidney function in nude mice and the H&E staining of their liver and kidney tissues. Result: PE significantly inhibited the growth of HepG2, BEL-7402, SMMC-7721 and Hep3B cells in a concentration- and time-dependent manner. Specifically, PE inhibited the growth of Hep3B cells by inducing apoptosis. PE treatment at the doses of 0.25, 0.5 and 1 g/kg for 21 days caused a respective 35.7 percent, 49.3 percent and 58.8 percent inhibition of the tumor volume, and a 14.8 percent, 40.2 percent and 55.6 percent decrease in the tumor weight, respectively, as compared with the vehicle group in tumor-loaded mice in vivo. PE promoted the release of cytochrome c from mitochondria to cytosol in a concentration-dependent manner. The expression levels of BAX (p
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- 2019
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20. Spatial Evolution, Driving Mechanism, and Patch Prediction of Grain-Producing Cultivated Land in China
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Zhiyuan Zhu, Jiajia Duan, Ruolan Li, and Yongzhong Feng
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China ,cultivated land ,grain production ,spatial evolution ,driving mechanism ,Agriculture (General) ,S1-972 - Abstract
China has implemented strict policies for protecting cultivated land, and the Chinese government has focused on the non-grain production (NGP) of cultivated land. This study aimed to analyze the spatial evolution law of grain-producing cultivated land (GPCL) in China between 2000 and 2018, explore the mechanism of GPCL, and simulate the spatial characteristics of GPCL in 2036. We used the Geographic Information System (GIS) and a patch-generating land-use simulation model, a new model that proposes a land expansion analysis strategy by improving previous rule-mining methods. China’s grain production rate (GPrate) shows a gradual upward trend between 2000 (36.98%) and 2018 (47.18%). The mutual conversion of GPCL and non-grain-producing cultivated land (NGPCL) are the primary transfer types. The evolution of GPCL is driven by climatic, economic, and social factors, of which population density is the most important factor. GPCL expansion patches are distributed in densely populated, economically developed, and warm and humid plain areas. Further, the simulation results showed that the GPrate in 2036 is estimated to be 41.39%, with GPCL transfer-in significantly exceeding the amount transferred out. Our results further cultivated land evolution-associated research and provide a basis for formulating scientific land-use policies for cultivated land protection for other countries.
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- 2022
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21. Research Progress on Heavy Metals Pollution in the Soil of Smelting Sites in China
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Muhammad Adnan, Baohua Xiao, Peiwen Xiao, Peng Zhao, Ruolan Li, and Shaheen Bibi
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heavy metal ,pollution ,soil ,smelting site ,hydrometallurgy ,resource recovery ,Chemical technology ,TP1-1185 - Abstract
Contamination by heavy metals is a significant issue worldwide. In recent decades, soil heavy metals pollutants in China had adverse impacts on soil quality and threatened food security and human health. Anthropogenic inputs mainly generate heavy metal contamination in China. In this review, the approaches were used in these investigations, focusing on geochemical strategies and metal isotope methods, particularly useful for determining the pathway of mining and smelting derived pollution in the soil. Our findings indicate that heavy metal distribution substantially impacts topsoils around mining and smelting sites, which release massive amounts of heavy metals into the environment. Furthermore, heavy metal contamination and related hazards posed by Pb, Cd, As, and Hg are more severe to plants, soil organisms, and humans. It’s worth observing that kids are particularly vulnerable to Pb toxicity. And this review also provides novel approaches to control and reduce the impacts of heavy metal pollution. Hydrometallurgy offers a potential method for extracting metals and removing potentially harmful heavy metals from waste to reduce pollution. However, environmentally friendly remediation of contaminated sites is a significant challenge. This paper also evaluates current technological advancements in the remediation of polluted soil, such as stabilization/solidification, natural attenuation, electrokinetic remediation, soil washing, and phytoremediation. The ability of biological approaches, especially phytoremediation, is cost-effective and favorable to the environment.
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- 2022
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22. A Network Pharmacology Approach to Investigate the Anticancer Mechanism and Potential Active Ingredients of Rheum palmatum L. Against Lung Cancer via Induction of Apoptosis
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Qing Zhang, Jia Liu, Ruolan Li, Rong Zhao, Mengmeng Zhang, Shujun Wei, Dong Ran, Wei Jin, and Chunjie Wu
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network pharmacology ,traditional Chinese medicine ,lung cancer ,apoptosis ,Rheum palmatum L. (Dahuang) ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Rheum palmatum L. (RPL) is a known traditional herbal medicine with the functions of “heat-clearing and damp-drying” in traditional Chinese medicine. Its anti-cancer effect against lung cancer has been confirmed previously, but the related mechanisms and active substances for its action has been little studied. This study adopted the network pharmacology, built the network map of drug ingredients and disease targets (DDN), and discussed the effective components of RPL and its possible mechanisms. All constituents of RPL were collected through database search and literature mining, and the potential active constituents were screened. The inverse pharmacophore matching model was used to predict the targets of active ingredients, and the method was supplemented by database retrieval and literature mining. Compounds-target data were inputted into Cytoscape software to build the DDN of RPL, and functional annotation analysis and pathway enrichment analysis were carried out. Finally, 20 active compounds were screened, which acted on 817 targets. A total of 22,418 lung cancer-related targets were collected, and 761 overlapped with drug targets. By bioinformatics annotation of these overlapping genes, a total of 235 gene ontology (GO) functional annotation analyses and 46 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were obtained. It was found that the enrichment of GO and KEGG was associated with apoptosis, suggesting RPL plays an anti-lung cancer role via inducing cell apoptosis. Subsequent cell experiment results showed RPL and its active constituents inhibited the proliferation of A549 cells and reduced clone formation rate of A549 cells via induction of apoptosis. In this study, the pharmacodynamic basis and mechanism of RPL against lung cancer were studied from the perspective of systematic pharmacology, which would be beneficial for further elucidating the anticancer effect of RPL on lung cancer.
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- 2020
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23. Guizhi-Shaoyao-Zhimu decoction possesses anti-arthritic effects on type II collagen-induced arthritis in rats via suppression of inflammatory reactions, inhibition of invasion & migration and induction of apoptosis in synovial fibroblasts
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Qing Zhang, Wei Peng, Shujun Wei, Daneng Wei, Ruolan Li, Jia Liu, Lanyu Peng, Shuang Yang, Yongxiang Gao, Chunjie Wu, and Xufeng Pu
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Guizhi-Shaoyao-Zhimu decoction ,Rheumatoid arthritis ,Apoptosis ,Synovial fibroblasts ,Anti-arthritic effect ,MH7A ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Rheumatoid arthritis (RA) is a known intractable chronic inflammatory disease of synovial joints characterized by hyperplasia and consecutive inflammation with a high prevalence.Guizhi-Shaoyao-Zhimu (GSZD) is the first choice for clinical treatment of RA in Chinese traditional medicine. This study is aimed to explore the possible pharmacological mechanisms of anti-arthritic effect of GSZD. Methods: Type II collagen-induced arthritis (CIA) rat model was used to study the anti-arthritic activity of GSZDin vivo, and toe swelling & arthritis score, serum levels of cytokines, and pathological examinations were carried out. In vitro, TNF-α induced MH7A cells were used to study the possible mechanisms of GSZD. The anti-proliferative effects of GSZD were determined by MMT assay, and pro-apoptotic activity of GSZD in MH7A cells was determined by flow cytometry analysis & DAPI staining. Furthermore, the adhesive and invasive abilities of MH7A cells were determined using cell adhesion and transwell assays. MMPs levels were determined by ELISA assays, and mRNA expressions of Caspase-3, -9, Bax, SOCS1, Bcl-2, JAK2, STAT-3 and -5 were determined using qRT-PCR analysis. Besides, the major chemical components in GSZD were analyzed by HPLC-QqQ-MS analysis. Results: Our results showed GSZD reduced the toe swelling & arthritis score, and serum levels of TNF-α, IL-1β, IL-6 & IL-17a in CIA rats; pathological examination results indicated GSZD improved ankle joint injury in CIA rats.In vitro, GSZD showed significant anti-proliferative and pro-apoptotic effects on TNF-α stimulated MH7A cells. After GSZD treatment, the adhesive and invasive abilities of MH7A cells were reduced, and secretions of MMPs, IL-6 and IL-8 were also reduced. GSZD decreased the releases of TNF-α and IL-1β in LPS stimulated RAW 264.7 cells. Further studies showed GSZD up-regulated mRNA expressions of Caspase-3, -9, Bax, and SOCS1, whereas down-regulated mRNA expressions of Bcl-2, JAK2, STAT3 and STAT5. Besides, 13 major chemical components were identified in GSZD extracts through HPLC-QqQ-MS analysis. Conclusion: Our results suggested GSZD possesses an anti-rheumatic effect on CIA rats, and the possible mechanism is related to inhibiting inflammatory response, inhibiting invasion and migration of synovial fibroblasts, and inducing apoptosis in synovial fibroblasts.
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- 2019
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24. Host-Induced Gene Silencing of a G Protein α Subunit Gene CsGpa1 Involved in Pathogen Appressoria Formation and Virulence Improves Tobacco Resistance to Ciboria shiraiana
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Panpan Zhu, Shuai Zhang, Ruolan Li, Changying Liu, Wei Fan, Tingzhang Hu, and Aichun Zhao
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appressorium formation ,Ciboria shiraiana ,G protein α subunit ,host-induced gene silencing (HIGS) ,mulberry ,pathogenicity ,Biology (General) ,QH301-705.5 - Abstract
Hypertrophy sorosis scleroteniosis caused by Ciboria shiraiana is the most devastating disease of mulberry fruit. However, few mulberry lines show any resistance to C. shiraiana. An increasing amount of research has shown that host-induced gene silencing (HIGS) is an effective strategy for enhancing plant tolerance to pathogens by silencing genes required for their pathogenicity. In this study, two G protein α subunit genes, CsGPA1 and CsGPA2, were identified from C. shiraiana. Silencing CsGPA1 and CsGPA2 had no effect on hyphal growth but reduced the number of sclerotia and increased the single sclerotium weight. Moreover, silencing CsGpa1 resulted in increased fungal resistance to osmotic and oxidative stresses. Compared with wild-type and empty vector strains, the number of appressoria was clearly lower in CsGPA1-silenced strains. Importantly, infection assays revealed that the virulence of CsGPA1-silenced strains was significantly reduced, which was accompanied by formation of fewer appressoria and decreased expression of several cAMP/PKA- or mitogen-activated protein-kinase-related genes. Additionally, transgenic Nicotiana benthamiana expressing double-stranded RNA targeted to CsGpa1 through the HIGS method significantly improved resistance to C. shiraiana. Our results indicate that CsGpa1 is an important regulator in appressoria formation and the pathogenicity of C. shiraiana. CsGpa1 is an efficient target to improve tolerance to C. shiraiana using HIGS technology.
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- 2021
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25. Simulating hyperelastic materials with anisotropic stiffness models in a particle-based framework.
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Tiancheng Wang, Yanrui Xu, Ruolan Li, Haoping Wang, Yuege Xiong, and Xiaokun Wang
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- 2023
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26. Design of a Robot for Automatically Hanging Ground Wires in Substations.
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Qingyu Pan, Xiaoyi Sun, Danyong Li, Ruolan Li, and Li Fan
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- 2022
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27. Substation work ticket identification and extraction algorithm based on multi-task model.
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Renjie Sui, Danyong Li, Ruolan Li, Li Fan, and Xiaoyi Sun
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- 2022
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28. Modelling the green supply chain of hotels based on front-back stage decoupling: perspective of ant colony labour division.
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Changbing Jiang, Ruolan Li, Jue Chen, Shufang Li, Tinggui Chen, and Chonghuan Xu
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- 2021
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29. A two-lane mixed traffic flow model with drivers' intention to change lane based on cellular automata.
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Changbing Jiang, Ruolan Li, Tinggui Chen, Chonghuan Xu, Liang Li, and Shufang Li
- Published
- 2020
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30. Input matters in the modeling of early phonetic learning.
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Ruolan Li, Thomas Schatz, Yevgen Matusevych, Sharon Goldwater, and Naomi Feldman
- Published
- 2020
31. FPGA-based real-time simulation of LCC-HVDC systems with C-NAM method
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Ruolan Li, Danyong Li, Yang Gao, Chen Gu, Xiaoyi Sun, and Li Fan
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Control and Systems Engineering ,Electrical and Electronic Engineering - Published
- 2023
32. Ellagic Acid–Cyclodextrin Inclusion Complex-Loaded Thiol–Ene Hydrogel with Antioxidant, Antibacterial, and Anti-inflammatory Properties for Wound Healing
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Tingyue Zhang, Liwei Guo, Ruolan Li, Jiang Shao, Lei Lu, Ping Yang, Ansha Zhao, and Yanqiu Liu
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General Materials Science - Published
- 2023
33. CoDiscard: A revenue model based cross-layer cooperative discarding mechanism for flash memory devices.
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Xiaoliu Feng, Xianzhang Chen, Ruolan Li, Jiali Li, Chunlin Song, Duo Liu, Yujuan Tan, and Lei Qiao
- Published
- 2022
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34. A Method to Identify Personal Desktop Activities.
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Ruolan Li, Huan Liao, Huili Su, Yukun Li, and Yongxuan Lai
- Published
- 2017
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35. Construction of extended ant colony labor division model for traffic signal timing and its application in mixed traffic flow model of single intersection.
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Changbing Jiang, Tinggui Chen, Ruolan Li, Liang Li, Gongfa Li, Chonghuan Xu, and Shufang Li
- Published
- 2020
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36. EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels
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Zhao, Qingqing Gao, Xia Li, Chunfan Xiang, Ruolan Li, Hongchun Xie, Jia Liu, Xiaoning Li, Guanghui Zhang, Shengchao Yang, Yanli Liang, Chenxi Zhai, and Yan
- Subjects
EbbHLH80 ,salt tolerance ,flavonoids ,ROS ,stress-responsive genes ,Erigeron breviscapus - Abstract
bHLH transcription factors are involved in multiple aspects of plant biology, such as the response to abiotic stress. Erigeron breviscapus is a composite plant, and its rich flavonoids have strong preventive and therapeutic effects on cardio cerebral vascular disease. EbbHLH80, a gene from E. breviscapus that positively regulates flavonoid synthesis, was previously characterized. However, it is unclear whether EbbHLH80 increases flavonoid accumulation, which affects salt tolerance. The function of EbbHLH80 in transgenic tobacco seeds was identified by phylogenetic analysis and metabolome-transcriptome analysis. We investigated the role of EbbHLH80 in salt stress response. Our results showed that the expression of EbbHLH80 increased following salt treatment. Integrating the metabolome and transcriptome analysis of EbbHLH80-OE and Yunyan 87 (WT) seeds, we identified several genes and metabolites related to flavonoid biosynthesis and salt stress. Moreover, EbbHLH80-OE plants displayed higher salt tolerance than wild-type plants during seed germination and seedling growth. After salt treatment, transgenic tobacco had significantly lower levels of reactive oxygen species (ROS) than WT, with enhanced levels of antioxidant enzyme expression. Altogether, our results demonstrated that EbbHLH80 might be a positive regulator, promoting salt tolerance by modulating ROS scavenging and increasing stress-responsive genes.
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- 2023
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37. Study on the Cone Angle of Sliding Sleeve Ball Seat in Open Hole Staged Ball Throwing Fracturing
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Ruolan Li, Dongsheng He, and Lang Xiong
- Abstract
In the ball type fracturing sliding sleeve, the contact surface between the ball socket and the fracturing ball is the most vulnerable part to failure. Once the contact surface fails, the sealing of the fracturing sliding sleeve will fail, the fracturing fluid will leak, and finally the fracturing will fail. The contact condition between the ball socket and the fracturing ball is mainly determined by the cone angle of the ball socket. In order to improve the contact condition between the ball socket and the fracturing ball as much as possible, and extend the service life of the sliding sleeve, based on fluid erosion and finite element structural analysis, this paper has carried out the analysis of the cone angle of the fracturing sliding sleeve. Firstly, the erosion of the ball seat is analyzed, and the erosion rate of the ball seat under different cone angles is obtained. The conclusion is that the erosion rate of the ball seat is proportional to the cone angle of the ball seat. Combined with the working conditions of the ball socket fracturing, the finite element analysis of the ball socket structure is carried out, and the stress conditions of different cone angles are compared. Finally, combined with the erosion rule and force analysis of the ball socket, the optimal cone angle of the ball socket is 20 °. When the cone angle of the ball socket is 20 °, the force of the ball socket meets the strength requirements of the ball socket, and can obtain a higher service life.
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- 2022
38. Advances on single Li-ion conducting polymer solid-state electrolytes
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Jiaxing Liu, Zhihao Yang, Weijie Liu, Zhengfei Yang, Shuyue Chen, Ruolan Li, Tieqi Huang, and Hongtao Liu
- Subjects
General Chemical Engineering ,Materials Chemistry ,General Chemistry ,Biochemistry - Published
- 2023
39. A machine learning assisted data placement mechanism for hybrid storage systems.
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Jinting Ren, Xianzhang Chen, Duo Liu, Yujuan Tan, Moming Duan, Ruolan Li, and Liang Liang 0002
- Published
- 2021
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40. Research on the Bridging Teaching Mode of College English and ESP of Flight Attendant Specialty based on SPOC
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Ruolan Li and Guangyong Liu
- Published
- 2023
41. Heavy metals in fish, rice, and human hair and health risk assessment in Wuhan city, central China
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Muhammad Ubaid Ali, Chuan Wang, Yuan Li, Ruolan Li, Shaochen Yang, Li Ding, Lin Feng, Bo Wang, Ping Li, and Ming Hung Wong
- Subjects
Health, Toxicology and Mutagenesis ,General Medicine ,Toxicology ,Pollution - Published
- 2023
42. A mulberry 9-cisepoxycarotenoid dioxygenase gene MaNCED1 is involved in plant growth regulation and confers salt and drought tolerance in transgenic tobacco.
- Author
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Panpan Zhu, Ruolan Li, Wei Fan, Zhongqiang Xia, Jun Li, Chuanhong Wang, and Aichun Zhao
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PLANT growth regulation ,MULBERRY ,DROUGHT tolerance ,GERMINATION ,ABSCISIC acid ,SODIUM tungstate ,ABIOTIC stress ,GENE expression - Abstract
The phytohormone abscisic acid (ABA) is vital in regulating root elongation, seed germination, and abiotic stress responses in plants. Conversely, the mechanisms of ABA in mulberry root growth, seed germination, and abiotic stress responses are poorly understood. Here, we reported that exogenous ABA and drought treatment inhibited the growth of mulberry seedlings but significantly increased the ratio of root/stem. Inhibition of ABA synthesis by fluridone and sodium tungstate resulted in the decrease of root/stem ratio. We also showed that the expression of MaNCED1 in the root was strongly induced by drought and salt stress. Increasing the expression of MaNCED1 in tobacco using overexpression leads to increased root elongation and reduced seed germination. Compared with the wild type, the accumulation of H
2 O2 and MDA was reduced, while the POD activity and proline content was increased in the transgenic plants after drought and salt treatment. Further studies revealed increased resistance to drought and salt stress in MaNCED1 overexpressed tobaccos. Meanwhile, the auxin and ethylene signal pathway-related gene expression levels increased in MaNCED1 overexpressed tobaccos. This study demonstrated the roles of mulberry MaNCED1 in regulating plant development and abiotic stress responses. It gave further insights into the coordinated regulation of ABA, auxin, and ethylene in seed growth and germination. [ABSTRACT FROM AUTHOR]- Published
- 2023
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43. Activation of Nrf2/HO-1 signaling: An important molecular mechanism of herbal medicine in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress
- Author
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Qing Zhang, Hu-Xinyue Duan, Wei Peng, Chunjie Wu, Jia Liu, and Ruolan Li
- Subjects
Multidisciplinary ,Future studies ,business.industry ,Pharmacology ,medicine.disease_cause ,Endothelial stem cell ,Apoptosis ,Nrf2 ho 1 ,Molecular mechanism ,Medicine ,Regulatory Pathway ,business ,Intracellular ,Oxidative stress - Abstract
Introduction Recently, Nrf2/HO-1 has received extensive attention as the main regulatory pathway of intracellular defense against oxidative stress and is considered an ideal target for alleviating endothelial cell (EC) injury. Objectives This paper aimed to summarized the natural monomers/extracts that potentially exert protective effects against oxidative stress in ECs. Methods A literature search was carried out regarding our topic with the keywords of “atherosclerosis” or “Nrf2/HO-1” or “vascular endothelial cells” or “oxidative stress” or “Herbal medicine” or “natural products” or “natural extracts” or “natural compounds” or “traditional Chinese medicines” based on classic books of herbal medicine and scientific databases including Pubmed, SciFinder, Scopus, the Web of Science, GoogleScholar, BaiduScholar, and others. Then, we analyzed the possible molecular mechanisms for different types of natural compounds in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress. In addition, perspectives for possible future studies are discussed. Results These agents with protective effects against oxidative stress in ECs mainly include phenylpropanoids, flavonoids, terpenoids, and alkaloids. Most of these agents alleviate cell apoptosis in ECs due to oxidative stress, and the mechanisms are related to Nrf2/HO-1 signaling activation. However, despite continued progress in research on various aspects of natural agents exerting protective effects against EC injury by activating Nrf2/HO-1 signaling, the development of new drugs for the treatment of atherosclerosis (AS) and other CVDs based on these agents will require more detailed preclinical and clinical studies. Conclusion Our present paper provides updated information of natural agents with protective activities on ECs against oxidative stress by activating Nrf2/HO-1. We hope this review will provide some directions for the further development of novel candidate drugs from natural agents for the treatment of AS and other CVDs.
- Published
- 2021
44. Antiepileptic Effects of Cicadae Periostracum on Mice and Its Antiapoptotic Effects in H2O2-Stimulated PC12 Cells via Regulation of PI3K/Akt/Nrf2 Signaling Pathways
- Author
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Ting Zhang, Ruolan Li, Jia Liu, Wei Peng, Jia-Yi Sun, Ting Tao, Chunjie Wu, and Qing Zhang
- Subjects
0301 basic medicine ,Aging ,Article Subject ,Cell ,Pharmacology ,Biochemistry ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Viability assay ,Protein kinase B ,PI3K/AKT/mTOR pathway ,chemistry.chemical_classification ,Reactive oxygen species ,QH573-671 ,biology ,Chemistry ,Glutathione peroxidase ,Cell Biology ,General Medicine ,030104 developmental biology ,medicine.anatomical_structure ,Apoptosis ,biology.protein ,Cytology ,human activities ,030217 neurology & neurosurgery - Abstract
Cicadae Periostracum (CPM), a commonly used animal traditional Chinese medicine (TCM), possesses antifebrile, spasmolytic, antiasthmatic, and antiphlogistic effects. In our present paper, we aimed to systemically investigate the antiepileptic effects of CPM in epileptic mice and explore the related molecular mechanism. Pentylenetetrazole- (PTZ) and strychnine-induced convulsion mice were established, and the results showed CPM could prolong the latency of convulsion and death and improve the neuronal damage in the hippocampus of PTZ-induced mice. Furthermore, the H2O2-treated PC12 cells were prepared to explore the possible mechanisms for the antiepileptic effects of CPM. CCK-8 results showed that CPM significantly improved the cell viability of H2O2-treated PC12 cells. Results of the acridine orange- (AO-) ethidium bromide (EB) staining, cell mitochondrial membrane potential (MOMP) analysis, and flow cytometry analysis showed that CPM significantly suppressed the H2O2-induced apoptosis in PC12 cells. In addition, CPM also downregulated the proapoptosis proteins, including Bax, cleaved- (C-) caspase-3, and C-caspase-9, and upregulated Bcl-2. Furthermore, CPM reduced the reactive oxygen species (ROS) levels via increasing antioxidative enzyme activities, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). Importantly, CPM could increase the phosphorylation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) in H2O2-induced PC12 cells and can promote the nuclear transfer of the nuclear factor E2-related factor 2 (Nrf2) and increase the expression of heme oxygenase-1 (HO-1) in the cytoplasm. In conclusion, our present study suggested CPM possessed antiepileptic effects through antiapoptosis of neuron cells via regulation of the PI3K/Akt/Nrf2 signaling pathway.
- Published
- 2021
45. In silico screening of anti-inflammatory constituents with good drug-like properties from twigs of Cinnamomum cassia based on molecular docking and network pharmacology
- Author
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Chunjie Wu, Jia Liu, Wei Peng, Xufeng Pu, Qing Zhang, Ruolan Li, and Yongxiang Gao
- Subjects
biology ,Chemistry ,In silico ,Pharmaceutical Science ,Computational biology ,biology.organism_classification ,Ligand (biochemistry) ,Cassia ,OMIM : Online Mendelian Inheritance in Man ,Pharmacology (medical) ,Target protein ,DrugBank ,Cinnamomum ,Discovery Studio - Abstract
Purpose: To investigate by in silico screening the anti-inflammatory constituents of Cinnamomum cassia twigs. Methods: Information on the constituents of C. cassia twigs was retrieved from the online Traditional Chinese Medicines (TCM) database and literature. Inflammation-related target proteins were identified from DrugBank, Online Mendelian Inheritance in Man (OMIM), Therapeutic Target Database (TTD), Genetic Association Database (GAD), and PharmGKB. The identified compounds were filtered by Lipinski’s rules with Discovery Studio software. The “Libdock” module was used to perform molecular docking; LibdockScores and default cutoff values for hydrogen bonds and van der Waals interactions were recorded. LibdockScores between the prototype ligand and target protein were set as the threshold; compounds with higher LibdockScores than threshold were regarded as active compounds. Cytoscape software was used to construct active constituent-target protein interaction networks. Results: Sixty-nine potential inflammatory constituents with good drug-like properties in C. cassia twigs were screened in silico based on molecular docking and network pharmacology analysis. JAK2, mPEGS-1, COX-2, IL-1β, and PPARγ were considered the five most important target proteins. Compounds such as methyl dihydromelilotoside, hierochin B, dihydromelilotoside, dehydrodiconiferyl alcohol, balanophonin, phenethyl (E)-3-[4-methoxyphenyl]-2-propenoate, quercetin, and luteolin each interacted with more than six of the selected target proteins. Conclusion: C. cassia twigs possess active compounds with good drug-like properties that can potentially be developed to treat inflammation with multi-components on multi-targets.
- Published
- 2021
46. Revealing and quantifying the effect of cattiite coprecipitation on the purity of K-struvite in aqueous solution
- Author
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Lin Wei, Tingting Zhang, Tianqiu Hong, Yugang Dong, Dongdong Ji, Lei Luo, Ruolan Li, Ziyue Li, and Yiming Tang
- Subjects
Process Chemistry and Technology ,Chemical Engineering (miscellaneous) ,Pollution ,Waste Management and Disposal - Published
- 2023
47. New protein tyrosine phosphatase inhibitors from fungus Aspergillus gorakhpurensis F07ZB1707
- Author
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Li Yeying, Tian-Hui Zhu, Yu-Cheng Gu, Ruolan Li, Mei Dong, Xinhua Lu, Yan-Nan Ji, Yufang Wang, Qing-Wen Shi, Xuexia Zhang, Guosheng Liu, Yan Fu, Qiqi Zhou, Francoise Sauriol, and Zhiyu Ni
- Subjects
biology ,010405 organic chemistry ,General Chemical Engineering ,General Chemistry ,Aspergillus gorakhpurensis ,Fungus ,Protein tyrosine phosphatase ,biology.organism_classification ,01 natural sciences ,In vitro ,0104 chemical sciences ,HeLa ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Xanthone ,Ic50 values ,Cytotoxicity - Abstract
Twelve new compounds, aspergorakhins A–L (1–12) coupled with one known xanthone leptosphaerin D (13), were isolated from the extract of soil-derived fungus Aspergillus gorakhpurensis F07ZB1707. Their structures were elucidated by spectroscopic data analysis including UV, IR, NMR, and HRESIMS. The absolute configurations of 5 and 8–11 were identified using ECD and OR calculations. All compounds were tested by enzyme inhibitory activity assay in vitro. Aspergorakhin A (1) showed selective activities against PTP1B and SHP1 over TCPTP with IC50 values 0.57, 1.19, and 22.97 μM, respectively. Compounds 1 and 2 exhibited modest cytotoxicity against tumor cell lines A549, HeLa, Bel-7402, and SMMC-7721 with IC50 values in the range of 6.75–83.4 μM.
- Published
- 2021
48. Boosting the photocatalytic hydrogen evolution and NO removal via synergistic effects of in-situ Pt NPs formation and titanium integration in Zr-Porphyrin MOFs
- Author
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Ruolan Li, Zongqian Wu, Ya Chen, Xingyan Liu, Weiwei Guo, Yumin Huang, Min Fu, and Youzhou He
- Subjects
Fuel Technology ,General Chemical Engineering ,Organic Chemistry ,Energy Engineering and Power Technology - Published
- 2023
49. Mercury migration to surface water from remediated mine waste and impacts of rainfall in a karst area – Evidence from Hg isotopes
- Author
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Junyao Yan, Ruolan Li, Muhammad Ubaid Ali, Chuan Wang, Bo Wang, Xingang Jin, Mingyu Shao, Ping Li, Leiming Zhang, and Xinbin Feng
- Subjects
Environmental Engineering ,Ecological Modeling ,Pollution ,Waste Management and Disposal ,Water Science and Technology ,Civil and Structural Engineering - Published
- 2023
50. Encapsulation of in-situ generated g-CNQDs with up-conversion effect in Zr/Ti-based porphyrin MOFs for efficient photocatalytic hydrogen production and NO removal
- Author
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Ruolan Li, Xingyan Liu, Yacen Tang, Jianning Yu, Ya Chen, Min Fu, Siqi Li, and Youzhou He
- Subjects
Filtration and Separation ,Analytical Chemistry - Published
- 2023
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