34 results on '"Ruo Xiao"'
Search Results
2. Hydrogen sulfide functions as a micro-modulator bound at the copper active site of Cu/Zn-SOD to regulate the catalytic activity of the enzyme
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Dong-Dong Wu, Sheng Jin, Ruo-Xiao Cheng, Wen-Jie Cai, Wen-Long Xue, Qing-Qing Zhang, Le-Jie Yang, Qi Zhu, Meng-Yao Li, Ge Lin, Yi-Zhen Wang, Xue-Pan Mu, Yu Wang, Igor Ying Zhang, Qi Zhang, Ying Chen, Sheng-Yang Cai, Bo Tan, Ye Li, Yun-Qian Chen, Pu-Juan Zhang, Chen Sun, Yue Yin, Ming-Jie Wang, Yi-Zhun Zhu, Bei-Bei Tao, Jia-Hai Zhou, Wei-Xue Huang, and Yi-Chun Zhu
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CP: Molecular biology ,Biology (General) ,QH301-705.5 - Abstract
Summary: The present study examines whether there is a mechanism beyond the current concept of post-translational modifications to regulate the function of a protein. A small gas molecule, hydrogen sulfide (H2S), was found to bind at active-site copper of Cu/Zn-SOD using a series of methods including radiolabeled binding assay, X-ray absorption near-edge structure (XANES), and crystallography. Such an H2S binding enhanced the electrostatic forces to guide the negatively charged substrate superoxide radicals to the catalytic copper ion, changed the geometry and energy of the frontier molecular orbitals of the active site, and subsequently facilitated the transfer of an electron from the superoxide radical to the catalytic copper ion and the breakage of the copper-His61 bridge. The physiological relevance of such an H2S effect was also examined in both in vitro and in vivo models where the cardioprotective effects of H2S were dependent on Cu/Zn-SOD.
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- 2023
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3. Antioxidants Attenuate Acute and Chronic Itch: Peripheral and Central Mechanisms of Oxidative Stress in Pruritus
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Zhou, Feng-Ming, Cheng, Ruo-Xiao, Wang, Shuai, Huang, Ya, Gao, Yong-Jing, Zhou, Yan, Liu, Teng-Teng, Wang, Xue-Long, Chen, Li-Hua, and Liu, Tong
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- 2017
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4. [Case-control study on Scarf and double metatarsal osteotomy of the first metatarsal for the treatment of hallux valgus deformity]
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Run, Tang, Jie, Yang, Xiao-Jun, Liang, Yi, Li, Jun-Hu, Wang, Yi-Xiang, Hao, and Ruo-Xiao, Zhang
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Male ,Treatment Outcome ,Case-Control Studies ,Humans ,Hallux ,Female ,Middle Aged ,Hallux Valgus ,Metatarsalgia ,Bunion ,Metatarsal Bones ,Aged ,Osteotomy - Abstract
To compare clinical efficacy of scarf osteotomy and double metatarsal osteotomy(DMO) in treating moderate to severe hallux valgus.Fifty patients (81 feet) with moderate to severe hallux valgus deformity were treated from January 2017 to December 2019, and were divided into Scarf osteotomy (SO) group or DMO group according to different osteotomy methods. There were 26 patients (44 feet) in SO group, including 1 male and 25 females, aged from 48 to 65 years old with an average of (55.5±4.67) years old;18 patients (30 feet) with moderate and 8 patients (14 feet) with severe. There were 24 patients(37 feet) in DMO group, including 1 male and 23 females, aged from 45 to 62 years old with an average of (52.1±6.8) years old;there were 14 patients (24 feet) with moderate and 10 patients (13 feet) with severe. Hallux valgus angle (HVA), intermetatarsal angle (IMA), distal metatarsal articular angle (DMAA) and relative length of the first metatarsal(RLFM) on weight-bearing anteroposterior radiographs before and after operation were compared. American Orthopaedic Foot and Ankle Society (AOFAS) hallux, metatarsal, and interphalangeal joint scores was used for evaluate clinical effects. Weight bearing time and complications were observed.Fivty patients were followed up. The follow-up time in SO group ranged from 12 to 36 months with an average of(20.50±6.22) months and from 16 to 28 months with an avaerge of(19.80±2.44) months in DMO group while there was no significant difference between two groups(Both of Scarf osteotomy and the first metatarsal biplane osteotomy could effectively treat moderate to severe hallux valgus deformity, with similar imaging and clinical evaluation. However, the relative length of the first metatarsal bone in SO group was longer than that in DMO group after operation, and the time of weight bearing in Scarf osteotomy is earlier than that of double metatarsal osteotomy of the first metatarsal.
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- 2022
5. The Phonation Factor in the Categorical Perception of Mandarin Tones.
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Ruo-Xiao Yang
- Published
- 2011
6. The influence of language experience on categorical perception of pitch contours.
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Gang Peng 0001, Hongying Zheng, Tao Gong 0001, Ruo-Xiao Yang, Jiangping Kong, and William S.-Y. Wang
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- 2010
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7. Restoring Spinal Noradrenergic Inhibitory Tone Attenuates Pain Hypersensitivity in a Rat Model of Parkinson’s Disease
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Lei-Fang Cao, Xiao-Yan Peng, Ya Huang, Bing Wang, Feng-Ming Zhou, Ruo-Xiao Cheng, Li-Hua Chen, Wei-Feng Luo, and Tong Liu
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Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
In the present study, we investigated whether restoring descending noradrenergic inhibitory tone can attenuate pain in a PD rat model, which was established by stereotaxic infusion of 6-hydroxydopamine (6-OHDA) into the bilateral striatum (CPu). PD rats developed thermal and mechanical hypersensitivity at the 4th week after surgery. HPLC analysis showed that NE content, but not dopamine or 5-HT, significantly decreased in lumbar spinal cord in PD rats. Additional noradrenergic depletion by injection of N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4) aggravated pain hypersensitivity in PD rats. At the 5th week after injection of 6-OHDA, systemic treatment with pharmacological norepinephrine (NE) precursor droxidopa (L-DOPS) or α2 adrenoceptor agonist clonidine significantly attenuated thermal and mechanical pain hypersensitivity in PD rats. Furthermore, application of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) reuptake inhibitors duloxetine, but not 5-HT selective reuptake inhibitors sertraline, significantly inhibited thermal and mechanical pain hypersensitivity in PD rats. Systemic administration of Madopar (L-DOPA) or the D2/D3 agonist pramipexole slightly inhibited the thermal, but not mechanical, hypersensitivity in PD rats. Thus, our study revealed that impairment of descending noradrenergic system may play a key role in PD-associated pain and restoring spinal noradrenergic inhibitory tone may serve as a novel strategy to manage PD-associated pain.
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- 2016
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8. Peripheral and spinal 5-HT receptors participate in cholestatic itch and antinociception induced by bile duct ligation in rats
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Tian, Bin, Wang, Xue-Long, Huang, Ya, Chen, Li-Hua, Cheng, Ruo-Xiao, Zhou, Feng-Ming, Guo, Ran, Li, Jun-Cheng, and Liu, Tong
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- 2016
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9. The influence of language experience on categorical perception of pitch contours
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Peng, Gang, Zheng, Hong-Ying, Gong, Tao, Yang, Ruo-Xiao, Kong, Jiang-Ping, and Wang, William S.-Y.
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- 2010
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10. The role of Nav1.7 and methylglyoxal-mediated activation of TRPA1 in itch and hypoalgesia in a murine model of type 1 diabetes
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Ya Huang, Li-Hua Chen, Di Liu, Ji Hu, Ruo-Xiao Cheng, Zhi-Hong Wang, Jiang-Tao Zhang, Tong Liu, Bing Wang, Cun-Jin Su, Ru-Rong Ji, and Yu Feng
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0301 basic medicine ,Genetically modified mouse ,Hypoalgesia ,Diabetic neuropathy ,Methylglyoxal ,Medicine (miscellaneous) ,Pharmacology ,Streptozotocin ,medicine.disease ,03 medical and health sciences ,chemistry.chemical_compound ,Transient receptor potential channel ,030104 developmental biology ,0302 clinical medicine ,chemistry ,Diabetes mellitus ,medicine ,Protein kinase A ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Methylglyoxal (MGO), an endogenous reactive carbonyl compound, plays a key role in the pathogenesis of diabetic neuropathy. The aim of this study is to investigate the role of MGO in diabetic itch and hypoalgesia, two common symptoms associated with diabetic neuropathy. Methods: Scratching behavior, mechanical itch (alloknesis), and thermal hypoalgesia were quantified after intradermal (i.d.) injection of MGO in naive mice or in diabetic mice induced by intraperitoneal (i.p.) injection of streptozotocin (STZ). Behavioral testing, patch-clamp recording, transgenic mice, and gene expression analysis were used to investigate the mechanisms underlying diabetic itch and hypoalgesia in mice. Results: I.d. injection of MGO evoked dose-dependent scratching in normal mice. Addition of MGO directly activated transient receptor potential ankyrin 1 (TRPA1) to induce inward currents and calcium influx in dorsal root ganglia (DRG) neurons or in TRPA1-expressing HEK293 cells. Mechanical itch, but not spontaneous itch was developed in STZ-induced diabetic mice. Genetic ablation of Trpa1 (Trpa1-/- ), pharmacological blockade of TRPA1 and Nav1.7, antioxidants, and mitogen-activated protein kinase kinase enzyme (MEK) inhibitor U0126 abrogated itch induced by MGO or in STZ-induced diabetic mice. Thermal hypoalgesia was induced by intrathecal (i.t.) injection of MGO or in STZ-induced diabetic mice, which was abolished by MGO scavengers, intrathecal injection of TRPA1 blockers, and in Trpa1-/- mice. Conclusion: This study revealed that Nav1.7 and MGO-mediated activation of TRPA1 play key roles in itch and hypoalgesia in a murine model of type 1 diabetes. Thereby, we provide a novel potential therapeutic strategy for the treatment of itch and hypoalgesia induced by diabetic neuropathy.
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- 2019
11. [VSD technique combined with
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Wen-Bo, Bai, Ruo-Xiao, Zhang, Jun-Kui, Xu, Jun, Lu, Xiao-Jun, Liang, and Jun-Hu, Wang
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Adult ,Male ,Young Adult ,Soft Tissue Injuries ,Treatment Outcome ,Drainage ,Humans ,Female ,Skin Transplantation ,Middle Aged ,Negative-Pressure Wound Therapy - Abstract
To explore the clinical effect of vacuum sealing drainage (VSD) technique andFrom November 2017 to April 2019, 21 patients (21 feet) with foot skin defect were treated with VSD technique andAll the 21 patients were followed up for 7 to 17 (10.8±2.7) months. There was no infection or nonunion in all patients. At the final follow-up, the skin margin of the healing site grew tightly, the skin was elastic, the texture was tough, the appearance was no obvious carbuncle. The time of wound healing was for 18 to 63 (41.2±13.3) days. Eight patients underwent stageⅡfixation or/and fusion, and all incisions healed by stageⅠ. According to Maryland's foot scoring, 9 cases got excellent results, 11 good, and 1 middle.VSD can effectively remove the necrotic tissue of the wound, provide a smooth drainage of the wound, combine with
- Published
- 2020
12. The role of Nav1.7 and methylglyoxal-mediated activation of TRPA1 in itch and hypoalgesia in a murine model of type 1 diabetes
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Cheng, Ruo-Xiao, primary, Feng, Yu, additional, Liu, Di, additional, Wang, Zhi-Hong, additional, Zhang, Jiang-Tao, additional, Chen, Li-Hua, additional, Su, Cun-Jin, additional, Wang, Bing, additional, Huang, Ya, additional, Ji, Ru-Rong, additional, Hu, Ji, additional, and Liu, Tong, additional
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- 2019
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13. Whole-Exome Sequencing Identifies a Variant inTMEM132ECausing Autosomal-Recessive Nonsyndromic Hearing Loss DFNB99
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Baichun Jiang, Yaoqin Gong, Pu Dai, Xiaohan Zhao, Shan Shan, Jiangxia Li, Yecheng Jin, Huijun Yuan, Qiji Liu, Changshun Shao, Ruo Xiao, Jingjing Xiao, Qingyan Zhang, and Qian Xin
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Male ,China ,Mutation, Missense ,Genes, Recessive ,Locus (genetics) ,Deafness ,Biology ,Mice ,otorhinolaryngologic diseases ,Genetics ,medicine ,Animals ,Humans ,Missense mutation ,Exome ,Zebrafish ,Genetics (clinical) ,Exome sequencing ,Chromosomes, Human, Pair 15 ,Gene knockdown ,Hair Cells, Auditory, Inner ,Genetic heterogeneity ,Membrane Proteins ,Sequence Analysis, DNA ,Zebrafish Proteins ,biology.organism_classification ,Molecular biology ,Phenotype ,Pedigree ,medicine.anatomical_structure ,Organ of Corti ,Gene Knockdown Techniques - Abstract
Autosomal-recessive nonsyndromic hearing loss (ARNSHL) features a high degree of genetic heterogeneity. Many genes responsible for ARNSHL have been identified or mapped. We previously mapped an ARNSHL locus at 17q12, herein designated DFNB99, in a consanguineous Chinese family. In this study, whole-exome sequencing revealed a homozygous missense mutation (c.1259G>A, p.Arg420Gln) in the gene-encoding transmembrane protein 132E (TMEM132E) as the causative variant. Immunofluorescence staining of the Organ of Corti showed Tmem132e highly expressed in murine inner hair cells. Furthermore, knockdown of the tmem132e ortholog in zebrafish affected the mechanotransduction of hair cells. Finally, wild-type human TMEM132E mRNA, but not the mRNA carrying the c.1259G>A mutation rescued the Tmem132e knockdown phenotype. We conclude that the variant in TMEM132E is the most likely cause of DFNB99.
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- 2014
14. Peripheral and spinal 5-HT receptors participate in cholestatic itch and antinociception induced by bile duct ligation in rats
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Jun-Cheng Li, Xue-Long Wang, Ran Guo, Ya Huang, Li-Hua Chen, Tong Liu, Bin Tian, Ruo-Xiao Cheng, and Feng-Ming Zhou
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0301 basic medicine ,Agonist ,Male ,medicine.medical_specialty ,medicine.drug_class ,Pain ,Pharmacology ,Serotonin 5-HT1 Receptor Antagonists ,Gastroenterology ,digestive system ,Article ,03 medical and health sciences ,0302 clinical medicine ,Cholestasis ,Internal medicine ,Peripheral Nervous System ,medicine ,Animals ,heterocyclic compounds ,Receptor ,5-HT receptor ,Multidisciplinary ,business.industry ,Pruritus ,Antagonist ,Scratching ,Serotonin 5-HT1 Receptor Agonists ,medicine.disease ,Spinal cord ,digestive system diseases ,Rats ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,nervous system ,Gene Expression Regulation ,Spinal Cord ,Peripheral nervous system ,Receptors, Serotonin ,business ,030217 neurology & neurosurgery - Abstract
Although 5-HT has been implicated in cholestatic itch and antinociception, two common phenomena in patients with cholestatic disease, the roles of 5-HT receptor subtypes are unclear. Herein, we investigated the roles of 5-HT receptors in itch and antinociception associated with cholestasis, which was induced by common bile duct ligation (BDL) in rats. 5-HT-induced enhanced scratching and antinociception to mechanical and heat stimuli were demonstrated in BDL rats. 5-HT level in the skin and spinal cord was significantly increased in BDL rats. Quantitative RT-PCR analysis showed 5-HT1B, 5-HT1D, 5-HT2A, 5-HT3A, 5-HT5B, 5-HT6, and 5-HT7 were up-regulated in peripheral nervous system and 5-HT1A, 5-HT1F, 5-HT2B, and 5-HT3A were down-regulated in the spinal cord of BDL rats. Intradermal 5-HT2, 5-HT3, and 5-HT7 receptor agonists induced scratching in BDL rats, whereas 5-HT3 agonist did not induce scratching in sham rats. 5-HT1A, 5-HT2, 5-HT3, and 5-HT7 agonists or antagonists suppressed itch in BDL rats. 5-HT1A agonist attenuated, but 5-HT1A antagonist enhanced antinociception in BDL rats. 5-HT2 and 5-HT3 agonists or antagonists attenuated antinociception in BDL rats. Our data suggested peripheral and central 5-HT system dynamically participated in itch and antinociception under cholestasis condition and targeting 5-HT receptors may be an effective treatment for cholestatic itch.
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- 2016
15. Six Mutations in AAGAB Confirm Its Pathogenic Role in Chinese Punctate Palmoplantar Keratoderma Patients
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Xuejun Zhang, Sen Yang, Da Lin, Tianwen Gao, Min Zhang, Ruo Xiao, Liangdan Sun, Hongzhou Cui, Wenjun Wang, Min Gao, Jun Wang, Jie Shen, Yi Zhou, Fengxia Liu, Hongyang Fu, Anping Zhang, Gang Chen, Yong Cui, Birong Guo, Zhen Yue, Wen-Ming Zhou, Qing Zhang, and Ying Dong
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Male ,medicine.medical_specialty ,Incidence ,Cell Biology ,Dermatology ,Biology ,Biochemistry ,Pedigree ,Adaptor Proteins, Vesicular Transport ,Asian People ,Keratoderma, Palmoplantar ,Mutation ,medicine ,Humans ,Female ,Carrier Proteins ,Punctate palmoplantar keratoderma ,Molecular Biology - Published
- 2013
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16. VSD technique combined with Bing Shi Yu Shang Ointment for the treatment of foot skin defects.
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BAI Wen-bo, ZHANG Ruo-xiao, XU Jun-kui, LU Jun, LIANG Xia-jun, and WANG Jun-hu
- Published
- 2020
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17. Antioxidants Attenuate Acute and Chronic Itch: Peripheral and Central Mechanisms of Oxidative Stress in Pruritus
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Ya Huang, Yan Zhou, Xue-Long Wang, Yong-Jing Gao, Li-Hua Chen, Ruo-Xiao Cheng, Teng-Teng Liu, Feng-Ming Zhou, Shuai Wang, and Tong Liu
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0301 basic medicine ,Central Nervous System ,Male ,Antioxidant ,Time Factors ,Physiology ,medicine.medical_treatment ,Pharmacology ,medicine.disease_cause ,Antioxidants ,Cyclic N-Oxides ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Chloroquine ,Malondialdehyde ,medicine ,Extracellular ,otorhinolaryngologic diseases ,Animals ,p-Methoxy-N-methylphenethylamine ,Peripheral Nerves ,skin and connective tissue diseases ,Extracellular Signal-Regulated MAP Kinases ,Cell Line, Transformed ,Skin ,Kidney ,Dose-Response Relationship, Drug ,Kinase ,business.industry ,Superoxide Dismutase ,General Neuroscience ,Pruritus ,General Medicine ,Spinal cord ,Acetylcysteine ,Disease Models, Animal ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,Anesthesia ,Systemic administration ,Original Article ,business ,Reactive Oxygen Species ,030217 neurology & neurosurgery ,Oxidative stress ,medicine.drug - Abstract
Itch (pruritus) is one of the most disabling syndromes in patients suffering from skin, liver, or kidney diseases. Our previous study highlighted a key role of oxidative stress in acute itch. Here, we evaluated the effects of antioxidants in mouse models of acute and chronic itch and explored the potential mechanisms. The effects of systemic administration of the antioxidants N-acetyl-L-cysteine (NAC) and N-tert-butyl-α-phenylnitrone (PBN) were determined by behavioral tests in mouse models of acute itch induced by compound 48/80 or chloroquine, and chronic itch by treatment with a mixture of acetone-diethyl-ether-water. We found that systemic administration of NAC or PBN significantly alleviated compound 48/80- and chloroquine-induced acute itch in a dose-dependent manner, attenuated dry skin-induced chronic itch, and suppressed oxidative stress in the affected skin. Antioxidants significantly decreased the accumulation of intracellular reactive oxygen species directly induced by compound 48/80 and chloroquine in the cultured dorsal root ganglia-derived cell line ND7-23. Finally, the antioxidants remarkably inhibited the compound 48/80-induced phosphorylation of extracellular signal-regulated kinase in the spinal cord. These results indicated that oxidative stress plays a critical role in acute and chronic itch in the periphery and spinal cord and antioxidant treatment may be a promising strategy for anti-itch therapy.
- Published
- 2016
18. Restoring Spinal Noradrenergic Inhibitory Tone Attenuates Pain Hypersensitivity in a Rat Model of Parkinson’s Disease
- Author
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Ya Huang, Tong Liu, Bing Wang, Weifeng Luo, Ruo-Xiao Cheng, Feng-Ming Zhou, Lei-Fang Cao, Li-Hua Chen, and Xiao-Yan Peng
- Subjects
Male ,0301 basic medicine ,Agonist ,Benzylamines ,Article Subject ,medicine.drug_class ,Pain ,Pharmacology ,lcsh:RC321-571 ,Levodopa ,Rats, Sprague-Dawley ,Norepinephrine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Dopamine ,medicine ,Animals ,Duloxetine ,Oxidopamine ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Pramipexole ,business.industry ,Parkinson Disease ,Corpus Striatum ,Clonidine ,Disease Models, Animal ,030104 developmental biology ,Spinal Cord ,Neurology ,chemistry ,Anesthesia ,Neurology (clinical) ,Droxidopa ,business ,030217 neurology & neurosurgery ,Research Article ,medicine.drug - Abstract
In the present study, we investigated whether restoring descending noradrenergic inhibitory tone can attenuate pain in a PD rat model, which was established by stereotaxic infusion of 6-hydroxydopamine (6-OHDA) into the bilateral striatum (CPu). PD rats developed thermal and mechanical hypersensitivity at the 4th week after surgery. HPLC analysis showed that NE content, but not dopamine or 5-HT, significantly decreased in lumbar spinal cord in PD rats. Additional noradrenergic depletion by injection of N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4) aggravated pain hypersensitivity in PD rats. At the 5th week after injection of 6-OHDA, systemic treatment with pharmacological norepinephrine (NE) precursor droxidopa (L-DOPS) orα2 adrenoceptor agonist clonidine significantly attenuated thermal and mechanical pain hypersensitivity in PD rats. Furthermore, application of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) reuptake inhibitors duloxetine, but not 5-HT selective reuptake inhibitors sertraline, significantly inhibited thermal and mechanical pain hypersensitivity in PD rats. Systemic administration of Madopar (L-DOPA) or the D2/D3 agonist pramipexole slightly inhibited the thermal, but not mechanical, hypersensitivity in PD rats. Thus, our study revealed that impairment of descending noradrenergic system may play a key role in PD-associated pain and restoring spinal noradrenergic inhibitory tone may serve as a novel strategy to manage PD-associated pain.
- Published
- 2016
19. The influence of language experience on categorical perception of pitch contours
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Hong-Ying Zheng, Tao Gong, Jiang-Ping Kong, Gang Peng, William S.-Y. Wang, and Ruo-Xiao Yang
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Linguistics and Language ,Categorical perception ,Tone (linguistics) ,Contrast (statistics) ,Mandarin Chinese ,Language and Linguistics ,language.human_language ,Linguistics ,Tone language ,German ,Speech and Hearing ,language ,Psychology ,Language Experience Approach - Abstract
Previous research on categorical perception of pitch contours has mainly considered the contrast between tone language and non-tone language listeners. This study investigates not only the influence of tone language vs. non-tone language experience (German vs. Chinese), but also the influence of different tone inventories (Mandarin tones vs. Cantonese tones), on the categorical perception of pitch contours. The results show that the positions of the identification boundaries do not differ significantly across the 3 groups of listeners, i.e., Mandarin, Cantonese, and German, but that the boundary widths do differ significantly between tone language (Mandarin and Cantonese) listeners and non-tone language (German) listeners, with broader boundary widths for non-tone language listeners. In the discrimination tasks, the German listeners exhibit only psychophysical boundaries, whereas Chinese listeners exhibit linguistic boundaries, and these linguistic boundaries are further shaped by the different tone inventories.
- Published
- 2010
20. Characterization and regulation of multiple forms of endo-1,4-β-glucanase genes during longan fruit growth and development
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Ruo Xiao, Jian-ye Chen, Min Ou, Wang-jin Lu, Hetong Lin, and Jian-fei Kuang
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chemistry.chemical_classification ,food and beverages ,Cellulase ,Horticulture ,Biology ,Sapindaceae ,Glucanase ,biology.organism_classification ,Enzyme ,chemistry ,Biochemistry ,Aril ,biology.protein ,Cell wall modification ,Gene ,Fruit tree - Abstract
Endo-1,4-β-glucanase (EGase, EC 3.2.1.4) is proposed to be involved in the control of cell wall modification. However, the relationship between EGase and longan fruit growth remains obscure. In the present work, the expression profiles of three Dl-EGase genes in pericarp and aril tissues of longan fruit during growth and development were characterized; moreover, the effects of plant growth substances, naphthalene acetic acid (NAA) and thidiazuron (TDZ) on their expressions of fruits at two different developmental stages were also investigated. The results showed that high levels of EGase activities in aril were in accordance with rapid aril growth, and three Dl-EGase genes exhibited different expression patterns in pericarp and aril during fruit growth and development. Dl-EGase1 and Dl-EGase3 seemed to be associated with the growth of both pericarp and aril, while Dl-EGase2 was closely related to pericarp growth. In addition, treatment of NAA and TDZ at the stage of pericarp growth increased the EGase activities and induced the expressions of all the three Dl-EGases. However, when NAA or TDZ was applied at the rapid aril growth stage, the accumulations of Dl-EGase1 and Dl-EGase2 in the pericarp, and the accumulations of Dl-EGase1 and Dl-EGase3 in the aril were induced. Furthermore, the expression patterns of the three Dl-EGases showed different tissue specificity. It is thus speculated that Dl-EGase genes played a different role in longan fruit growth and were developmentally regulated, and more importantly, the responses of Dl-EGases to plant growth substances were different and dependent on fruit development stage and fruit tissue.
- Published
- 2009
21. Expression analysis of endo-1,4-β-glucanase genes during aril breakdown of harvested longan fruit
- Author
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Min Ou, Jian-ye Chen, Hetong Lin, Wang-jin Lu, Yueming Jiang, Ruo Xiao, and Jian-wen Chen
- Subjects
Nutrition and Dietetics ,Biology ,Glucanase ,Enzyme assay ,Horticulture ,Aril ,Botany ,Gene expression ,Expression analysis ,biology.protein ,Agronomy and Crop Science ,Gene ,Softening ,Food Science ,Biotechnology - Abstract
BACKGROUND: The aim of the present study was to investigate the expression profiles of three endo-1,4-β-glucanase (EGase, EC 3.2.1.4) genes during aril breakdown of longan fruit stored at room temperature (25 °C), low temperature (10 °C) or on transferring fruit stored at 10 °C for 20 days to 25 °C. RESULTS: Three longan full-length cDNAs, designated Dl-EGase1, Dl-EGase2 and Dl-EGase3, were isolated and characterized. EGase activity in aril tissues of longan fruit increased with the appearance of aril breakdown symptoms, while RNA gel blot analysis revealed that the accumulations of three Dl-EGase genes exhibited differential characteristics with the occurrence of aril breakdown. Dl-EGase2 may be involved in the aril breakdown in longan fruit at the later stage of storage at room temperature. Conversely, expression of Dl-EGase3 could be mainly involved in aril breakdown of fruit stored at 10 °C. In addition, Dl-EGase3 and Dl-Egase2 were related to the aril breakdown of fruit transferred from low temperature to room temperature. CONCLUSION: The results obtained in this study indicate that Dl-EGase genes are involved in the aril breakdown of longan fruit and that considerable variation exists between expression patterns of individual members of the EGase gene family. Copyright © 2009 Society of Chemical Industry
- Published
- 2009
22. Expression of a phenylalanine ammonia-lyase gene in relation to aril breakdown in harvested longan fruit
- Author
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Xue-Wu Duan, Wang-jin Lu, Ruo Xiao, Yueming Jiang, Min Ou, Jian-ye Chen, and Hetong Lin
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chemistry.chemical_classification ,biology ,Cold storage ,Phenylalanine ,Phenylalanine ammonia-lyase ,Horticulture ,humanities ,Enzyme assay ,Enzyme ,Biochemistry ,chemistry ,Aril ,Complementary DNA ,Gene expression ,Genetics ,biology.protein - Abstract
SummaryA full-length phenylalanine ammonia-lyase gene (PAL) cDNA, named DlPAL1, was isolated and characterised from harvested longan fruit. The expression pattern of DlPAL1 and PAL activities in relation to aril breakdown in longan fruit stored at different temperatures were investigated. The results showed that both PAL activity and the accumulation of DlPAL1 mRNA increased during storage at 25oC and at 10oC, which coincided with an increase in the aril breakdown index (ABI). The increase in PAL activity or in the accumulation of DlPAL1 mRNA coincided with the increase in ABI in fruit stored at 4oC for 40 d, then transferred to 25oC for 2 d. No accumulation of DlPAL1 mRNA throughout storage at 4oC could be detected, although the increase in PAL activity was in accordance with the increase in ABI. Furthermore, changes in DlPAL1 mRNA accumulation were not fully in accordance with the changes observed in PAL activity, and a rapid increase in PAL activity in arils was observed as aril breakdown occurred rapi...
- Published
- 2009
23. Expansin and XET Genes Are Differentially Expressed During Aril Breakdown in Harvested Longan Fruit
- Author
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Jian-fei Kuang, Hetong Lin, Ruo Xiao, Hai-ling Feng, Wang-jin Lu, Hui Xie, Min Ou, Jian-Ye Chen, Yueming Jiang, and Yu-xiong Zhong
- Subjects
Dimocarpus ,biology ,Chemistry ,Horticulture ,Xyloglucan endotransglucosylase ,biology.organism_classification ,Expansin ,Aril ,Botany ,Genetics ,Molecular mechanism ,Browning ,Gene - Abstract
Fresh fruit of longan (Dimocarpus longan Lour.) are susceptible to pericarp browning and aril breakdown. Aril breakdown in longan fruit is regarded as one of the most important factors reducing quality and shortening storage life of the fruit. To better understand the molecular mechanism of aril breakdown, the expression patterns of three expansin (EXP) and three xyloglucan endotransglucosylase (XET) genes in relation to the aril breakdown of longan fruit stored at room temperature (25 °C) or low temperature (4 °C) were investigated. The results showed that aril breakdown index increased progressively during storage at 25 and at 4 °C. Northern blotting analysis revealed that the accumulations of three EXP and three XET genes exhibited differential characteristics with the occurrence of aril breakdown. During storage at 25 °C, the accumulations of Dl-XET3 increased after 1 day, suggesting that Dl-XET3 correlated well with the early aril breakdown, while Dl-EXP3 together with Dl-XET1 and Dl-XET2 was involved in later aril breakdown. However, expression of Dl-XET1 and Dl-XET2 could be mainly involved in aril breakdown of longan fruit stored at 4 °C. In addition, Dl-EXP2, whose accumulation increased sharply when longan fruit were transferred from low temperature to room temperature within 12 hours, was related to the aril breakdown in this storage period. These data indicated that Dl-EXPs and Dl-XETs were closely related to aril breakdown in longan fruit.
- Published
- 2008
24. Antioxidants Attenuate Acute and Chronic Itch: Peripheral and Central Mechanisms of Oxidative Stress in Pruritus
- Author
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Zhou, Feng-Ming, primary, Cheng, Ruo-Xiao, additional, Wang, Shuai, additional, Huang, Ya, additional, Gao, Yong-Jing, additional, Zhou, Yan, additional, Liu, Teng-Teng, additional, Wang, Xue-Long, additional, Chen, Li-Hua, additional, and Liu, Tong, additional
- Published
- 2016
- Full Text
- View/download PDF
25. Restoring Spinal Noradrenergic Inhibitory Tone Attenuates Pain Hypersensitivity in a Rat Model of Parkinson’s Disease
- Author
-
Cao, Lei-Fang, primary, Peng, Xiao-Yan, additional, Huang, Ya, additional, Wang, Bing, additional, Zhou, Feng-Ming, additional, Cheng, Ruo-Xiao, additional, Chen, Li-Hua, additional, Luo, Wei-Feng, additional, and Liu, Tong, additional
- Published
- 2016
- Full Text
- View/download PDF
26. Rapid gene identification in a Chinese osteopetrosis family by whole exome sequencing
- Author
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Fengyan Li, Jianguo Zhang, Peng Zhu, Yue Zhang, Hua Lin, Wen Xue, Xiaohua Meng, Ruo Xiao, Jiejing Chen, Lijuan Wang, Minglin Ou, Yong Dai, Jinlong Liang, Weiguo Sui, Wei Liu, Min Ding, and Xiaohua Pan
- Subjects
Adult ,Male ,Candidate gene ,Time Factors ,Positional cloning ,symbols.namesake ,Young Adult ,Asian People ,Genetics ,medicine ,Humans ,Exome ,Family ,Exome sequencing ,Genetic Association Studies ,Sanger sequencing ,biology ,Genetic heterogeneity ,High-Throughput Nucleotide Sequencing ,Osteopetrosis ,General Medicine ,Middle Aged ,medicine.disease ,Pedigree ,Child, Preschool ,Mutation (genetic algorithm) ,symbols ,biology.protein ,Female ,CLCN7 - Abstract
Osteopetrosis is a rare genetically heterogeneous disorder of bone metabolism characterized by increased skeleton density. In the past, standard methods for genetic diagnosis of osteopetrosis have primarily been performed by candidate gene screening and positional cloning. However, these methods are time and labor consumptive; and the genetic basis of approximately 30% of the cases is yet to be elucidated. Here, we employed whole exome sequencing of two affected individuals from an osteopetrosis family to identify a candidate mutation in CLCN7 (Y99C). It was identified from a total of 1757 and 1728 genetic variations found in either patient, which were then distilled using filtering strategies and confirmed using Sanger sequencing. We identified this mutation in six family members, while not in population matched controls. This mutation was previously found in osteopetrosis patients by other researchers. Our evolutionary analysis also indicated that it is under extremely high selective pressure, and is likely to be critical for the correct function of ClC-7, and thus is likely to be the responsible cause of disease. Collectively, our data further indicated that mutation (Y99C) may be a cause of osteopetrosis, and highlights the use of whole exome sequencing as a valuable approach to identifying disease mutations in a cost and time efficient manner.
- Published
- 2012
27. Exome sequencing identifies a COL14A1 mutation in a large Chinese pedigree with punctate palmoplantar keratoderma
- Author
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Xuejun Zhang, Bi-Rong Guo, Ruo Xiao, Yuan Liu, Ping Li, Xu Yang, Jun Zhu, Xin Zhang, Xiaoguang Zhang, Min Li, Min Gao, Liangdan Sun, Lin Yang, Jun Wang, Huayang Tang, Feng-Ping Xu, Ming Li, Gang Chen, Yong Cui, Xianbo Zuo, Xianfa Tang, Hui Cheng, Jian-Guo Zhang, Xiaodong Zheng, Sen Yang, Jianjun Liu, Jianbo Wang, Wei-Dong Du, and Qing Zhang
- Subjects
Adult ,Male ,China ,DNA Mutational Analysis ,Molecular Sequence Data ,Locus (genetics) ,Biology ,Polymorphism, Single Nucleotide ,symbols.namesake ,Asian People ,Genetic linkage ,Keratoderma, Palmoplantar ,Genetics ,medicine ,Missense mutation ,Humans ,Exome ,Amino Acid Sequence ,Keratoderma ,Gene ,Genetics (clinical) ,Exome sequencing ,Glycoproteins ,Sanger sequencing ,Genome, Human ,medicine.disease ,Human genetics ,Pedigree ,Mutation ,symbols ,Female ,Collagen - Abstract
Background Punctate palmoplantar keratoderma (PPPK) is a rare autosomal dominant skin disorder characterised by numerous hyperkeratotic papules irregularly distributed on the palms and soles. To date, no causal gene for this disease has been identified. Methods We performed exome sequencing analysis of four affected individuals and two unaffected controls from one Chinese PPPK family where disease locus was mapped at 8q24.13-8q24.21 by our previous linkage analysis. Results We identified a novel heterozygous mutation in COL14A1 gene (c.4505C→T (p.Pro1502Leu)), which located within the linkage region that we previously identified for PPPK. The mutation was shared by the four affected individuals, but not for the two controls of the family. Sanger sequencing confirmed this mutation in another four cases from this family. This mutation was invisible in the normal controls of this family as well as the additional 676 unrelated normal controls and 781 patients with other disease. The shared COL14A1 mutation, p.Pro1502Leu, is a missense substitution at a highly conserved amino acid residue across multiple species. Conclusions The power of combining exome sequencing and linkage information in the study of genetics of autosomal dominant disorders, even in simplex cases, has been demonstrated. Our results suggested that COL14A1 would be a casual gene for PPPK, which was helpful for advancing us on understanding of the pathogenesis of PPPK.
- Published
- 2012
28. Loss-of-function mutations in HOXC13 cause pure hair and nail ectodermal dysplasia
- Author
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Ruoyu Li, Quan Chen, Xue Zhang, Zhanli Tang, Huijun Wang, Zhimiao Lin, Jinghua Yin, Liu Xuanzhu, Yong Yang, Xuejun Zhu, Ruo Xiao, Jie Zhang, Regina C. Betz, Kathrin A. Giehl, Lingshen Wu, Lei Shi, Mingyang Lee, and Lanlan Dai
- Subjects
Male ,Ectodermal dysplasia ,China ,Nonsense mutation ,Genes, Recessive ,Mice, Transgenic ,Biology ,Hypotrichosis ,Hair keratin ,Exon ,Consanguinity ,Mice ,Nail Diseases ,Asian People ,Ectodermal Dysplasia ,Report ,medicine ,Genetics ,Animals ,Humans ,Genetics(clinical) ,Exome ,Genetics (clinical) ,Skin ,Homeodomain Proteins ,integumentary system ,Gene Expression Profiling ,Homozygote ,medicine.disease ,Molecular biology ,Pedigree ,medicine.anatomical_structure ,Phenotype ,Gene Expression Regulation ,Nail disease ,Chromosomal region ,Nail (anatomy) ,Female - Abstract
Pure hair and nail ectodermal dysplasia (PHNED) is a congenital condition characterized by hypotrichosis and nail dystrophy. Autosomal-recessive PHNED has previously been mapped to chromosomal region 12q12-q14.1, which contains the type II hair keratin and HOXC clusters. Hoxc13-null mice are known to develop hair and nail defects very similar to those seen in human PHNED. We performed whole-exome sequencing in a consanguineous Chinese family affected by PHNED and identified a homozygous nonsense mutation (c.390CA [p.Tyr130(∗)]) in HOXC13 in all affected individuals. In an additional affected female from a consanguineous Afghan family, we found a 27.6 kb homozygous microdeletion involving the first exon of HOXC13. We examined HOXC13 expression in scalp specimen obtained from the index individual of the Chinese family and detected dramatically reduced mRNA levels in skin tissue and nearly absent protein staining in hair follicles, suggesting a mechanism of nonsense-mediated mRNA decay. We also observed markedly decreased expression of four HOXC13 target genes in the specimen. Taken together, our results demonstrate that loss-of-function mutations in HOXC13 cause autosomal-recessive PHNED and further highlight the importance of HOXC13 in hair and nail development.
- Published
- 2012
29. Exome sequencing identifies a COL14A1 mutation in a large Chinese pedigree with punctate palmoplantar keratoderma.
- Author
-
Bi-Rong Guo, Xin Zhang, Gang Chen, Jian-Guo Zhang, Liang-Dan Sun, Wei-dong Du, Qing Zhang, Yong Cui, Jun Zhu, Xian-Fa Tang, Ruo Xiao, Yuan Liu, Min Li, Hua-Yang Tang, Xu Yang, Hui Cheng, Ming Li, Min Gao, Ping Li, and Jian-Bo Wang
- Subjects
PAPILLON Lefevre syndrome ,GENETIC mutation ,LOCUS (Genetics) ,AMINO acids - Abstract
Background Punctate palmoplantar keratoderma (PPPK) is a rare autosomal dominant skin disorder characterised by numerous hyperkeratotic papules irregularly distributed on the palms and soles. To date, no causal gene for this disease has been identified. Methods We performed exome sequencing analysis of four affected individuals and two unaffected controls from one Chinese PPPK family where disease locus was mapped at 8q24.13-8q24.21 by our previous linkage analysis. Results We identified a novel heterozygous mutation in COL14A1 gene (c.4505C→T ( p.Pro1502Leu)), which located within the linkage region that we previously identified for PPPK. The mutation was shared by the four affected individuals, but not for the two controls of the family. Sanger sequencing confirmed this mutation in another four cases from this family. This mutation was invisible in the normal controls of this family as well as the additional 676 unrelated normal controls and 781 patients with other disease. The shared COL14A1 mutation, p.Pro1502Leu, is a missense substitution at a highly conserved amino acid residue across multiple species. Conclusions The power of combining exome sequencing and linkage information in the study of genetics of autosomal dominant disorders, even in simplex cases, has been demonstrated. Our results suggested that COL14A1 would be a casual gene for PPPK, which was helpful for advancing us on understanding of the pathogenesis of PPPK. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
30. Expression of a phenylalanine ammonia-lyase gene in relation to aril breakdown in harvested longan fruit.
- Author
-
Min Ou, Ruo Xiao, Jian-Ye Chen, He-Tong Lin, Xue-Wu Duan, Yue-Ming Jiang, and Wang-Jin Lu
- Subjects
PHENYLALANINE ammonia lyase ,MESSENGER RNA ,LONGAN ,PHENYLALANINE metabolism ,PLANT metabolites - Abstract
A full-length phenylalanine animonia-lyase gene (PAL) eDNA, named DIPAL1 , was isolated and characterised from harvested longan fruit. The expression pattern of DIPAL1 and PAL activities in relation to aril breakdown in longan fruit stored at different temperatures were investigated. The results showed that both PAL activity and the accumulation of DlPAL1 mRNA increased during storage at 25°C and at 10°C, which coincided with an increase in the aril breakdown index (ABI). The increase in PAL activity or in the accumulation of DIPAL1 mRNA coincided with the increase in ABI in fruit stored at 4°C for 40 d, then transferred to 25°C for 2 d. No accumulation of DIPAL1 mRNA throughout storage at 4°C could be detected, although the increase in PAL activity was in accordance with the increase in ABI. Furthermore, changes in DlPAL1 mRNA accumulation were not fully in accordance with the changes observed in PAL activity, and a rapid increase in PAL activity in arils was observed as an! breakdown occurred rapidly whether fruit were stored at 25°C, 4°C, or 10°C, or moved from 4°C to 25°C. These results suggested that PAL activity is closely involved in aril breakdown in longan fruit, and that PAL activity might be regulated at the posttranscriptional level during aril breakdown. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
31. Expansin and XET Genes Are Differentially Expressed During Aril Breakdown in Harvested Longan Fruit.
- Author
-
Yu-Xiong Zhong, Jian-Ye Chen, Hai-Ling Feng, Jian-Fei Kuang, Ruo Xiao, Min Ou, Hui Xie, Wang-Jin Lu, Yue-Ming Jiang, and He-Tong Lin
- Subjects
- *
FRUIT , *LOW temperatures , *HARVESTING , *FARM produce storage , *SCIENTIFIC method , *MANAGEMENT , *COOKING ,FRUIT genetics - Abstract
Fresh fruit of longan (Dimocarpus longan Lour.) are susceptible to pericarp browning and aril breakdown. Aril breakdown in longan fruit is regarded as one of the most important factors reducing quality and shortening storage life of the fruit. To better understand the molecular mechanism of aril breakdown, the expression patterns of three expansin (EXP) and three xyloglucan endotransglucosylase (XET) genes in relation to the aril breakdown of longan fruit stored at room temperature (25 °C) or low temperature (4 °C) were investigated. The results showed that aril breakdown index increased progressively during storage at 25 and at 4 °C. Northern blotting analysis revealed that the accumulations of three EXP and three XET genes exhibited differential characteristics with the occurrence of aril breakdown. During storage at 25 °C, the accumulations of Dl-XET3 increased after 1 day, suggesting that Dl-XET3 correlated well with the early aril breakdown, while Dl-EXP3 together with Dl-XET1 and Dl-XET2 was involved in later aril breakdown. However, expression of Dl-XET1 and Dl-XET2 could be mainly involved in aril breakdown of longan fruit stored at 4 °C. In addition, Dl-EXP2, whose accumulation increased sharply when longan fruit were transferred from low temperature to room temperature within 12 hours, was related to the aril breakdown in this storage period. These data indicated that Dl-EXPs and Dl-XETs were closely related to aril breakdown in longan fruit. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
32. [Case-control study on Scarf and double metatarsal osteotomy of the first metatarsal for the treatment of hallux valgus deformity].
- Author
-
Tang R, Yang J, Liang XJ, Li Y, Wang JH, Hao YX, and Zhang RX
- Subjects
- Female, Humans, Male, Middle Aged, Aged, Case-Control Studies, Treatment Outcome, Osteotomy methods, Hallux Valgus surgery, Hallux, Metatarsal Bones surgery, Bunion, Metatarsalgia
- Abstract
Objective: To compare clinical efficacy of scarf osteotomy and double metatarsal osteotomy(DMO) in treating moderate to severe hallux valgus., Methods: Fifty patients (81 feet) with moderate to severe hallux valgus deformity were treated from January 2017 to December 2019, and were divided into Scarf osteotomy (SO) group or DMO group according to different osteotomy methods. There were 26 patients (44 feet) in SO group, including 1 male and 25 females, aged from 48 to 65 years old with an average of (55.5±4.67) years old;18 patients (30 feet) with moderate and 8 patients (14 feet) with severe. There were 24 patients(37 feet) in DMO group, including 1 male and 23 females, aged from 45 to 62 years old with an average of (52.1±6.8) years old;there were 14 patients (24 feet) with moderate and 10 patients (13 feet) with severe. Hallux valgus angle (HVA), intermetatarsal angle (IMA), distal metatarsal articular angle (DMAA) and relative length of the first metatarsal(RLFM) on weight-bearing anteroposterior radiographs before and after operation were compared. American Orthopaedic Foot and Ankle Society (AOFAS) hallux, metatarsal, and interphalangeal joint scores was used for evaluate clinical effects. Weight bearing time and complications were observed., Results: Fivty patients were followed up. The follow-up time in SO group ranged from 12 to 36 months with an average of(20.50±6.22) months and from 16 to 28 months with an avaerge of(19.80±2.44) months in DMO group while there was no significant difference between two groups( P >0.05). All incision healed at stage I, and osteotomy healed at (20.31±3.17) months after operation. One patient occurred hallux adduction deformity, without transferred metatarsalgia;2 patients occurred metastatic metatarsalgia in DMO group. There were no significant difference in HVA, IMA, DMAA, and AOFAS scores between two groups( P >0.05) before and after operation. There was no significant difference in RLFM between two groups( P >0.05), while had significant difference in RLFM between two groups at final follow-up ( P <0.05). Partial weight-bearing time and full weight-bearing time in SO group were significantly earlier than those in DMO group ( P <0.05)., Conclusion: Both of Scarf osteotomy and the first metatarsal biplane osteotomy could effectively treat moderate to severe hallux valgus deformity, with similar imaging and clinical evaluation. However, the relative length of the first metatarsal bone in SO group was longer than that in DMO group after operation, and the time of weight bearing in Scarf osteotomy is earlier than that of double metatarsal osteotomy of the first metatarsal.
- Published
- 2022
- Full Text
- View/download PDF
33. [VSD technique combined with Bing Shi Yu Shang Ointment for the treatment of foot skin defects].
- Author
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Bai WB, Zhang RX, Xu JK, Lu J, Liang XJ, and Wang JH
- Subjects
- Adult, Drainage, Female, Humans, Male, Middle Aged, Skin Transplantation, Treatment Outcome, Young Adult, Negative-Pressure Wound Therapy, Soft Tissue Injuries surgery
- Abstract
Objective: To explore the clinical effect of vacuum sealing drainage (VSD) technique and Bing Shi Yu Shang Ointment in the treatment of foot skin defect., Methods: From November 2017 to April 2019, 21 patients (21 feet) with foot skin defect were treated with VSD technique and Bing Shi Yu Shang Ointment. There were 17 males and 4 females, aged from 24 to 60 years old with an average of (37.8±11.2) years, 9 cases caused by traffic accident injury, 6 cases by heavy injury, 6 cases by falling injury. The time from injury to operation was for 3 to 36 (8.6±7.2) h, and the area of foot skin defect was for 20.35 to 83.43(47.2±19.5) cm
2 . All patients underwent debridement or phaseⅠtemporary fixation in emergency, and three-dimensional imagingof the foot was performed by using Mimics software, and the defect area was rendered. The quality of wound healing and complications were observed, and the clinical effect was evaluated by Maryland foot function score., Results: All the 21 patients were followed up for 7 to 17 (10.8±2.7) months. There was no infection or nonunion in all patients. At the final follow-up, the skin margin of the healing site grew tightly, the skin was elastic, the texture was tough, the appearance was no obvious carbuncle. The time of wound healing was for 18 to 63 (41.2±13.3) days. Eight patients underwent stageⅡfixation or/and fusion, and all incisions healed by stageⅠ. According to Maryland's foot scoring, 9 cases got excellent results, 11 good, and 1 middle., Conclusion: VSD can effectively remove the necrotic tissue of the wound, provide a smooth drainage of the wound, combine with Bing Shi Yu Shang Ointment to prevent infection and promote the rapid growth of granulation tissue, whose whole treatment cycle was short, the wound healing site was highquality, the limb function was good, and the clinical effect was satisfactory.- Published
- 2020
- Full Text
- View/download PDF
34. The role of Na v 1.7 and methylglyoxal-mediated activation of TRPA1 in itch and hypoalgesia in a murine model of type 1 diabetes.
- Author
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Cheng RX, Feng Y, Liu D, Wang ZH, Zhang JT, Chen LH, Su CJ, Wang B, Huang Y, Ji RR, Hu J, and Liu T
- Subjects
- Animals, Diabetic Neuropathies etiology, Diabetic Neuropathies genetics, Diabetic Neuropathies metabolism, Disease Models, Animal, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Inbred ICR, Mice, Knockout, NAV1.7 Voltage-Gated Sodium Channel genetics, NAV1.7 Voltage-Gated Sodium Channel metabolism, Neuralgia etiology, Neuralgia genetics, Pruritus etiology, Pruritus genetics, Pyruvaldehyde adverse effects, TRPA1 Cation Channel genetics, Diabetes Mellitus, Type 1 complications, Neuralgia metabolism, Pruritus metabolism, TRPA1 Cation Channel metabolism
- Abstract
Methylglyoxal (MGO), an endogenous reactive carbonyl compound, plays a key role in the pathogenesis of diabetic neuropathy. The aim of this study is to investigate the role of MGO in diabetic itch and hypoalgesia, two common symptoms associated with diabetic neuropathy. Methods : Scratching behavior, mechanical itch (alloknesis), and thermal hypoalgesia were quantified after intradermal (i.d.) injection of MGO in naïve mice or in diabetic mice induced by intraperitoneal (i.p.) injection of streptozotocin (STZ). Behavioral testing, patch-clamp recording, transgenic mice, and gene expression analysis were used to investigate the mechanisms underlying diabetic itch and hypoalgesia in mice. Results : I.d. injection of MGO evoked dose-dependent scratching in normal mice. Addition of MGO directly activated transient receptor potential ankyrin 1 (TRPA1) to induce inward currents and calcium influx in dorsal root ganglia (DRG) neurons or in TRPA1-expressing HEK293 cells. Mechanical itch, but not spontaneous itch was developed in STZ-induced diabetic mice. Genetic ablation of Trpa1 ( Trpa1
-/- ), pharmacological blockade of TRPA1 and Nav 1.7, antioxidants, and mitogen-activated protein kinase kinase enzyme (MEK) inhibitor U0126 abrogated itch induced by MGO or in STZ-induced diabetic mice. Thermal hypoalgesia was induced by intrathecal (i.t.) injection of MGO or in STZ-induced diabetic mice, which was abolished by MGO scavengers, intrathecal injection of TRPA1 blockers, and in Trpa1-/- mice. Conclusion : This study revealed that Nav 1.7 and MGO-mediated activation of TRPA1 play key roles in itch and hypoalgesia in a murine model of type 1 diabetes. Thereby, we provide a novel potential therapeutic strategy for the treatment of itch and hypoalgesia induced by diabetic neuropathy., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.- Published
- 2019
- Full Text
- View/download PDF
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