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11. Changes in adiponectin expression in acute myocardial infarction rats and the significance of bisoprolol intervention

Author

Zhang, Song, He, Ben, Goldstein, Steven, Ge, Junbo, Wang, Zuyue, and Ruiz, George

Subjects
Heart attack -- Drug therapy, Immunohistochemistry -- Research, Peptide hormones -- Health aspects, Bisoprolol -- Health aspects, DNA polymerases, Biological sciences
Abstract

The aims of this study were to explore the changes in expression of myocardial adiponectin (APN), changes in serum APN, and the significance of bisoprolol intervention in acute myocardial infarction (AMI) rats. An AMI rat model was established for the purposes of this study and was used for analysis of serum APN as determined by ELISA. Changes in expression of myocardial APN mRNA and APN protein in AMI rats were determined via reverse transcriptase (RT)-PCR and immunohistochemistry. Serum APN concentration and APN protein expression of the myocardium decreased significantly in the AMI groups compared with the sham operation group, with the lowest serum APN and APN protein expression on day 7 after AMI. On days 7 and 10 after AMI, the expression of myocardial APN mRNA in the AMI groups decreased significantly compared with the sham operation group. However, the APN mRNA increased on day 10 compared with that on day 7. Notably, there was an increase in levels of serum APN and myocardial APN expression after bisoprolol intervention. The expression of myocardial APN and serum APN decreased in AMI rats. APN may be an important protective factor against AMI. Bisoprolol can also protect against AMI because it increases APN expression. Key words: myocardial infarction, adiponectin, bisoprolol, rat, RT-PCR, protein expression, immunohistochemistry. La presente etude a eu pour but d'examiner les modifications de l'expression de l'APN myocardique et de l'APN serique, ainsi que le role d'un traitement au bisopropol chez des rats presentant un infarctus aigu du myocardique (IAM). Nous avons realise un modele de rats IAM aux fins de l'etude. Nous avons analyse l'APN serique determineie par ELISA. Nous avons evaluee les modifications de l'expression de l'ARNm de l'APN et de la proteine dans le myocarde, par RT-PCR et immunohistochimie. La concentration de l'APN serique et l'expression de la proteine APN myocardique ont diminue significativement chez les groupes IAM par rapport a celles du groupe temoin opere de maniere fictive; l'expression de l'APN serique et de la proteine APN a ete minimale le jour 7 post-IAM. Les jours 7 et 10 post-IAM, l'expression de l'ARNm de l'APN myocardique a diminue de maniere significative chez les groupes IAM par rapport a celle du groupe temoin opere de maniere fictive. Toutefois, l'expression de l'ARNm de l'APN a augmente; davantage le jour 10 que le jour 7. Les taux d'APN serique et l'expression de l'APN myocardique ont augmente; apres le traitement au bisoprolol. L'expression de l'APN myocardique et de l'APN serique a diminue chez les rats IAM. L'APN pourrait etre un important facteur de protection contre l'IAM. Le bisoprolol pourrait aussi offrir une protection contre l'IAM etant donne qu'il augmente l'expression de l'APN. Mots-cles: infarctus du myocarde, adiponectine, bisoprolol, rat, RT-PCR, expression proteique, immunohistochimie. [Traduit par la Redaction], Introduction Adiponectin (APN) is a polypeptide hormone that plays an important role in anti-atherosclerosis, anti-inflammatory, and antithrombosis pathways. Recent studies have shown that APN is correlated with genetic factors such [...]

Published
2011
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17. The expression and significance of proto-oncogene c-fos in viral myocarditis

Author

Goldstein Steven, He Ben, Zhang Song, Ge Junbo, Wang Zuyue, and Ruiz George

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background c-fos may play a role in the pathogenesis of some diseases. The expression and function of c-fos in viral myocarditis (VMC) have not yet been reported. To study the change and significance of proto-oncogene c-fos in VMC is the objective of this experiment. Methods An animal model of VMC was established via coxsackie virus B3 inoculation. VMC mice were then treated with a c-fos monoclonal antibody and isoproterenol and the protein and mRNA expression of c-fos were studied via immunohistochemical analysis and in situ hybridization. Results were simultaneously analyzed for the significance of c-fos expression in mice with VMC. Results Myocardial necrosis and cell infiltration decreased after treatment with c-fos monoclonal antibody compared to control mice, while myocardial necrosis and cell infiltration were increased after treatment with isoproterenol. Positive cardiomyocytes with c-Fos expression increased at 3, 5, 7, 9, and 15 days after virus inoculation in VMC mice compared to control mice, while returning to almost normal levels at 35 days. The expression level of c-fos mRNA at 3 and 7 days after virus inoculation in VMC mice was also higher than that of control mice. Conclusions c-fos expression in the cardiomyocytes of VMC mice is significantly increased, c-fos plays an important role in myocardial lesions. The apparent increase in expression of c-fos is likely to be involved in the pathogenesis of VMC.

Published
2010
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19. Secretome analysis of Trypanosoma cruzi by proteomics studies

Author

Brossas, Jean-Yves, Gulin, Julián Ernesto Nicolás, Bisio, Margarita Maria Catalina, Chapelle, Manuel, Marinach-Patrice, Carine, Bordessoules, Mallaury, Palazon Ruiz, George, Vion, Jeremy, Paris, Luc, Altcheh, Jaime, and Mazier, Dominique

Subjects
Proteomics, Life Cycles, Serum Proteins, Cell Lines, Protozoan Proteins, lcsh:Medicine, Ciencias de la Salud, Protozoology, Biochemistry, Heat Shock Response, Tandem Mass Spectrometry, Chlorocebus aethiops, Medicine and Health Sciences, Parasitología, purl.org/becyt/ford/3.4 [https], lcsh:Science, Cellular Stress Responses, Protozoans, Trypanosoma Cruzi, Eukaryota, purl.org/becyt/ford/3.1 [https], Bioquímica y Biología Molecular, Medicina Básica, Cell Processes, Protozoan Life Cycles, Electrophoresis, Polyacrylamide Gel, purl.org/becyt/ford/3 [https], Biological Cultures, Research Article, Neglected Tropical Diseases, Trypanosoma, CIENCIAS MÉDICAS Y DE LA SALUD, Trypanosoma cruzi, Tecnologías que involucran la identificación de ADN, proteínas y enzimas, y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar, Research and Analysis Methods, Microbiology, Biotecnología de la Salud, purl.org/becyt/ford/3.3 [https], Virology, parasitic diseases, Parasitic Diseases, Animals, Chagas Disease, Vero Cells, Protozoan Infections, lcsh:R, Host Cells, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Trypomastigotes, Tropical Diseases, Parasitic Protozoans, lcsh:Q, Parasitic Disease, Viral Transmission and Infection, Developmental Biology, Chromatography, Liquid
Abstract

Background: Chagas disease is a debilitating often fatal disease resulting from infection by the protozoan parasite Trypanosoma cruzi. Chagas disease is endemic in 21 countries of the Americas, and it is an emerging disease in other countries as a result of migration. Given the chronic nature of the infection where intracellular parasites persist for years, the diagnosis of T. cruzi by direct detection is difficult, whereas serologic tests though sensitive may yield false-positive results. The development of new rapid test based on the identification of soluble parasitic antigens in serum would be a real innovation in the diagnosis of Chagas disease. Methods: To identify new soluble biomarkers that may improve diagnostic tests, we investigated the proteins secreted by T. cruzi using mass spectrometric analyses of conditioned culture media devoid of serum collected during the emergence of trypomastigotes from infected Vero cells. In addition, we compared the secretomes of two T. cruzi strains from DTU Tc VI (VD and CL Brener). Results: Analysis of the secretome collected during the emergence of trypomastigotes from Vero cells led to the identification of 591 T. cruzi proteins. Three hundred sixty three proteins are common to both strains and most belong to different multigenic super families (i.e. TcS, GP63, MASP, and DGF1). Ultimately we have established a list of 94 secreted proteins, common to both DTU Tc VI strains that do not belong to members of multigene families. Conclusions: This study provides the first comparative analysis of the secretomes from two distinct T. cruzi strains of DTU TcVI. This led us to identify a subset of common secreted proteins that could potentially serve as serum markers for T. cruzi infection. Their potential could now be evaluated, with specific antibodies using sera collected from patients and residents from endemic regions. Fil: Brossas, Jean Yves. Inserm; Francia. Universite de Paris VI; Francia. Hôpital Pitié-Salpêtrière; Francia Fil: Gulin, Julián Ernesto Nicolás. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas.; Argentina Fil: Bisio, Margarita María Catalina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas.; Argentina Fil: Chapelle, Manuel. Bruker Daltonics; Francia Fil: Marinach Patrice, Carine. Inserm; Francia. Universite de Paris VI; Francia Fil: Bordessoulles, Mallaury. Universite de Paris VI; Francia. Inserm; Francia Fil: Palazon Ruiz, George. Inserm; Francia Fil: Vion, Jeremy. Inserm; Francia Fil: Paris, Luc. Hôpital Pitié-Salpêtrière; Francia. Universite de Paris VI; Francia Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas.; Argentina Fil: Mazier, Dominique. Inserm; Francia. Universite de Paris VI; Francia. Hôpital Pitié-Salpêtrière; Francia

Published
2017

20. Organizers did great job at Southeast Regionals

Author

Alicea-Ruiz, George

Subjects
Sports and fitness
Abstract

I want to thank Joel O'Connor and his staff for hosting the USHA Southeast Regional Championships in Nashville, Tenn. The facilities were fantastic. It would be nice to have the [...]

Published
2019

25. Secretome analysis of Trypanosoma cruzi by proteomics studies

Author

Brossas, Jean-Yves, primary, Gulin, Julián Ernesto Nicolás, additional, Bisio, Margarita Maria Catalina, additional, Chapelle, Manuel, additional, Marinach-Patrice, Carine, additional, Bordessoules, Mallaury, additional, Palazon Ruiz, George, additional, Vion, Jeremy, additional, Paris, Luc, additional, Altcheh, Jaime, additional, and Mazier, Dominique, additional

Published
2017
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30. Classification of Non-Indigenous Species Based on Their Impacts: Considerations for Application in Marine Management

Author

Ojaveer, Henn, Galil, Bella S., Campbell, Marnie L., Carlton, James T., Canning-Clode, Joao, Cook, Elizabeth J., Davidson, Alisha D., Hewitt, Chad L., Jelmert, Anders, Marchini, Agnese, McKenzie, Cynthia H., Minchin, Dan, Occhipinti-Ambrogi, Anna, Olenin, Sergej, Ruiz, George, Ojaveer, Henn, Galil, Bella S., Campbell, Marnie L., Carlton, James T., Canning-Clode, Joao, Cook, Elizabeth J., Davidson, Alisha D., Hewitt, Chad L., Jelmert, Anders, Marchini, Agnese, McKenzie, Cynthia H., Minchin, Dan, Occhipinti-Ambrogi, Anna, Olenin, Sergej, and Ruiz, George

Abstract

Assessment of the ecological and economic/societal impacts of the introduction of non-indigenous species (NIS) is one of the primary focus areas of bioinvasion science in terrestrial and aquatic environments, and is considered essential to management. A classification system of NIS, based on the magnitude of their environmental impacts, was recently proposed to assist management. Here, we consider the potential application of this classification scheme to the marine environment, and offer a complementary framework focussing on value sets in order to explicitly address marine management concerns. Since existing data on marine NIS impacts are scarce and successful marine removals are rare, we propose that management of marine NIS adopt a precautionary approach, which not only would emphasise preventing new incursions through pre-border and at-border controls but also should influence the categorisation of impacts. The study of marine invasion impacts requires urgent attention and significant investment, since we lack the luxury of waiting for the knowledge base to be acquired before the window of opportunity closes for feasible management.

Published
2015

32. Embedding Palliative Care into Advanced Heart Failure Teams: A Model for Success

Author

Kathryn A. Walker, Hunter Groninger, Ruiz George, and Mahesh Chandrasekhar

Subjects
medicine.medical_specialty, Palliative care, business.industry, 030204 cardiovascular system & hematology, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, Heart failure, Medicine, 030212 general & internal medicine, Medical emergency, Cardiology and Cardiovascular Medicine, business, Intensive care medicine
Published
2016

33. 183 Characteristics of Gastrointestinal Bleeding and Subsequent Endoscopic Therapy After Implantation of Left Ventricular Assist Device for End Stage Heart Failure

Author

Mahajan, Sandeep, primary, Majure, David T., additional, Taefi, Amir, additional, Salcedo, Julio, additional, Rodrigo, Maria E., additional, Hofmeyer, Mark, additional, Ruiz, George, additional, Molina, Ezequiel, additional, Boyce, Steven W., additional, Najjar, Samer, additional, and Sheikh, Farooq, additional

Published
2015
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34. Tu1038 Interferon-Free Hepatitis C Treatment in LVAD Patients With End-Stage Heart Failure: New Hope for Heart Transplantation

Author

Fishbein, Dawn A., primary, Geboy, Alexander, additional, Ruiz, Maria Elena, additional, Boyce, Steven W., additional, Molina, Ezequiel, additional, Ayodele, Adebisi, additional, Cha, Hyun A., additional, Perez, Idene E., additional, Bannerman, Renee, additional, Mcdonnell, Erin, additional, Hofmeyer, Mark, additional, Rodrigo, Maria E., additional, Ruiz, George, additional, Sheikh, Farooq, additional, Najjar, Samer, additional, and Majure, David T., additional

Published
2015
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35. Prolonged cold ischemia: risk factor for acute rejection in renal grafting of cadaveric kidney transplantation

Author

Oscar Manuel, Ruiz-George, Benjamín, Trujillo-Hernández, Rebeca Olivia, Millán-Guerrero, and Clemente, Vásquez-Jiménez

Subjects
Adult, Graft Rejection, Adolescent, Cold Ischemia, Delayed Graft Function, Middle Aged, Kidney, Kidney Transplantation, Tissue Donors, Young Adult, Cross-Sectional Studies, Risk Factors, Acute Disease, Cadaver, Living Donors, Tissue and Organ Harvesting, Humans, Drug Therapy, Combination, Mexico, Immunosuppressive Agents, Retrospective Studies
Abstract

Exposure of renal grafting to prolonged cold ischemia time (CIT) and the association with acute rejection (AR) are known. However, there is no evidence in Mexico about this topic. Thus, the objective of this study was to evaluate prolonged CIT as a risk factor for AR in renal grafting of cadaveric kidney transplantation in a Mexican population.A cross-sectional study was carried out. Clinical files of patients undergoing renal grafting using cadaveric kidneys were reviewed from July 1994-June 2004. Prolonged CIT (=12 h) as a risk factor for AR was evaluated. Other related variables were also examined.From 425 kidney transplantations, only 33 cases were cadaveric. Ten patients had AR. Prolonged CIT (OR 8.4; CI 1.5-44.2, p = 0.02) and azathioprine (AZA)-prednisone (PDN)-cyclosporine (CSA) combination (OR 9.1; CI 1.5-49.4, p = 0.02) were risk factors for AR. Anti-CD25 use (OR 0.6; CI 0.009-0.37, p = 0.001) and mycofenolate mofetil (MMF)-PDN-CSA combination (OR 0.1; CI 0.02-0.65, p = 0.02) were protective factors for AR.In a Mexican population, prolonged CIT and AZA-PDN-CSA combination were risk factors for AR. Meanwhile, anti- CD25 use and MMF-PDN-CSA combination were protective factors for AR in cadaveric kidney transplantations.

Published
2009

36. Isquemia fría prolongada, factor para rechazo agudo del injerto en trasplante renal cadavérico

Author

Oscar Manuel Ruiz-George, Benjamín Trujillo-Hernández, Rebeca Olivia Millán-Guerrero, and Clemente Vásquez-Jiménez

Subjects
Trasplante renal, Medicina, rechazo agudo, injerto
Abstract

Introducción: Es conocido que la exposición del injerto renal a tiempo prolongado de isquemia fría se asocia con rechazo agudo. Dado que no se encontró evidencia del tema en México, el objetivo de este estudio fue determinar el papel del tiempo de isquemia fría prolongado sobre el injerto en el trasplante renal cadavérico en población mexicana. Material y métodos: Estudio observacional, retrospectivo, transversal y analítico para el que se seleccionaron los expedientes de pacientes con trasplante renal entre julio de 1994 y junio de 2004. Se realizó análisis de diferentes variables para determinar su efecto sobre el rechazo agudo, entre ellas el tiempo prolongado de isquemia fría ( 12 horas). Resultados: De los 425 transplantes realizados, 33 fueron de donador cadavérico; 10 pacientes tuvieron rechazo agudo. El tiempo prolongado de isquemia fría (OR = 8.4, IC = 1.5-44.2, p = 0.02) y la combinación azatioprina (AZA)-prednisona (PDN)- ciclosporina (CSA) (OR = 9.1, IC = 1.5-49.4, p = 0.02) fueron factores de riesgo para rechazo agudo. El uso de antiCD25 (OR = 0.6, IC = .009-0.37, p = 0.001) y la combinación mofetil micofenolato (MMF)-PDN-CSA (OR = 0.1, IC = 0.02-0.65, p = 0.02) fueron factores protectores de rechazo agudo. Conclusiones: En una población mexicana, el tiempo de isquemia fría prolongado y la combinación AZA-PDN-CSA fueron factores de riesgo para rechazo agudo, mientras que el uso de antiCD25 y la combinación MMF-PDN-CSA fueron protectores para rechazo agudo en trasplantes renales de donadores cadavéricos.

Published
2009

50. Tahoe Trailblazing.

Author

RUIZ, GEORGE

Abstract

The article focuses on the Tahoe Rim Trail Endurance Runs on the east shore of Lake Tahoe, Nevada and states that runner Jesse Haynes trailed in third at the 50-mile split, Adam Camarena ran a very strong second 50-mile split to come home third and Roxanne Woodhouse ran to her third victory.

Published
2016

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