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RAS plays an important role in the carcinogenesis of colorectal epithelial cells, but RAS mutations are only present, at most, in approximately 50% of patients. In order to compare the gene mutation profiles of patients with or without a KRAS mutation, the clinicopathological data of 858 patients and NGS test results of 1697 patients with colorectal cancer were used in this analysis. In 858 patients, the mutation rate of KRAS was 50.8% (i.e., 436/858). Only 2 out of 349 (0.5%) KRAS mutant patients had a BRAF mutation, while 25 out of 422 (5.9%) KRAS wild-type patients had a BRAF mutation (p

Phase linear characteristic is an important parameter of RF transceiver module in phased array radar system. This paper analyzes the causes of phase nonlinear based on the mechanism of which, and puts forward four methods of optimizing the phase linear. Finally, an X-band RF transceiver module is designed and manufactured to verify the effectiveness of the methods. The test results show that by using the methods proposed, the phase distortion of the RF transceiver module can be improved obviously.

Colorectal cancer (CRC) is one of the most common malignancies in the world. Studies have demonstrated that single nucleotide polymorphisms (SNPs) in microRNA genes (miRSNPs) are involved in the occurrence of cancers. However, the relationship between the miRSNPs within the terminal-loops of microRNA precursors and the development of CRC is still largely unknown. In this study, we found that a miRSNP rs7911488 T>C in the terminal-loop of pre-miR-1307 was significantly associated with the occurrence of CRC. The C allele of rs7911488 is more prevalent in CRC patients than in healthy controls (P < 0.001), and this C allele prevalence is related to low level of miR-1307 expression. A RNA-binding protein MBNL1 binds with a 'UGCUGC' motif in the terminal-loop of the C-allelic pre-miR-1307 and blocks Dicer processing, resulting in downregulation of miR-1307 expression. Consequently, the antiapoptosis protein Bcl2, which is a direct target of miR-1307, is overexpressed in CRC. Furthermore, MBNL1 participates in processing of both C-allelic and T-allelic pre- miR-1307. In summary, our results show that rs7911488 C-allelic pre-miR-1307 binds to MBNL1 and infers with Dicer processing, leading to reduced miR-1307 and increased Bcl2 expression, thus representing an important process in the initiation of CRC.

A number of single nucleotide polymorphisms (SNPs) within the 3′-UTR of genes have been proved to be contributed to the risk of cancers. Here, we determined an SNP rs4819388 in the 3′-UTR of B7-H2 gene in 183 gastric cancer patients and 348 healthy controls by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis results showed that the rs4819388 genotypes were significantly related to the occurrence of gastric cancer. Compared with the GG homozygotes, the GA heterozygotes were significantly more prevalent in the patients (OR = 1.60, 95%CI = 1.08–2.37). In addition, the A allelic frequencies were significantly higher than that of the G allele in the patients with lymphatic metastasis ( p = 0.034) and/or advanced TNM stage ( p = 0.032). Dual-luciferase reporter assays showed that miR-24 inhibited the expression of B7-H2 through binding with the B7-H2 3′-UTR, and this inhibitory role of miR-24 was impacted by rs4819388. Our findings suggest that the SNP rs4819388 in B7-H2 3′-UTR, through disrupting the regulatory role of miR-24 on B7-H2 expression, contributes to the occurrence of gastric cancer.

This paper presents a Ka band multi-beam phased array for communication satellite. Antenna element is carried out as a double circular polarization horn. Phased array working at 23 GHz of transmitting frequency and 26 GHz of receiving frequency is constructed with triangle grid arrangement. The transmitting part has 320 channels, while the receiving part comprises 1280 channels. The phased array could provide 4 transmitting beams and 8 receiving beams synchronously. Scanning angle range is better than 13 degree of conical. Equivalent isotropic radiated power(EIRP) achieves 56 dBW for each beam, and its G/T is better than 19 dB/K. Performances of the element and array are simulated, which shows good properties for the system design.

A number of single-nucleotide polymorphisms within the 3'-UTR of genes have been shown to relate to the occurrence of cancers. In this study, by using polymerase chain reaction-restriction fragment length analysis method, we determined an SNP rs1599795 in the 3'-UTR of CD80 gene in 183 gastric cancer patients and 348 healthy controls. Statistical analysis results showed that SNP rs1599795 genotypes were significantly correlated with the risk of gastric cancer. Compared with the AA homozygotes, the TA heterozygotes were significantly more prevalent in the patients (OR 1.44, 95 % CI 0.98-2.11) with a larger tumor size (P = 0.001), deeper infiltration (P = 1.5 × 10(-5)), higher possibility of lymph node metastasis (P = 0.003), and more in the late stage (TNM stage III and IV; P = 0.003); the TT homozygotes had larger tumor size (P = 0.001) and lower degree of differentiation (P = 2.2 × 10(-4)). Dual-luciferase reporter assays showed that miR-132-3p, miR-212-3p, and miR-361-5p inhibited the expression of CD80 through binding with the CD80 3'-UTR, and this inhibitory role of miR-132-3p, miR-212-3p, and miR-361-5p was impacted by rs1599795. Our findings have shown that the SNP rs1599795 in CD80 3'-UTR, through disrupting the regulatory role of miR-132-3p, miR-212-3p, and miR-361-5p in CD80 expression, contributed to the occurrence of gastric cancer.

Computer-aided process planning (CAPP) performs an important function, integrating product design with production, but has not kept pace with CAD/CAM developments. More and more attention has been paid to CAPP automation, intelligence and CAD/CAPP/CAM integration, and a plethora of prototype systems has been developed. However, CAPP applications are less than satisfactory. With the objective of improving CAPP usability and adaptability, this paper proposes object-oriented manufacturing resources modelling (OOMRM) and agent-based process planning (AAPP). The OOMRM exceeds the framework of traditional manufacturing resource databases. It describes manufacturing resources capability and capacity in an object-oriented manner, which intends to encapsulate manufacturing system knowledge and the methods of using the knowledge. Based on the OOMRM, an AAPP prototype is implemented as a man-machine integrated process planning platform. It will support an experienced manufacturing engineer in mapping out a more reas...

Co-inhibitor B7-H1 expresses in various cancers and contributes to cancer immune evasion by inhibiting T cell activation and proliferation, yet the regulatory mechanisms for B7-H1 over-expression in cancers remain largely unknown. Here, the expression of B7-H1 and PTEN proteins were firstly detected by using immunohistochemistry method. B7-H1 immunoreactivities were found in 54.5% (55/101) of the colorectal cancer tissues with no expression in the normal tissues, and the PTEN protein immunoreactivities were observed in 51.5% (52/101) of the colorectal cancer tissues and 72.3% (73/101) of the normal tissues. Statistical analysis results indicated that the B7-H1 expression was negatively correlated to the PTEN expression in colorectal cancer (p=0.001). Then the expressions of microRNAs (miRNAs) in six pairs of colorectal cancer and normal tissues were determined by miRNA array, and 30 up-regulated miRNAs were found in the colorectal cancer tissues. Finally, the impact of these up-regulated miRNAs on PTEN expression was tested by using dual-luciferase reporter assay system, from which the results indicated that miR-20b, -21, and -130b were involved in suppression of PTEN expression. These findings suggest that miR-20b, -21, and -130b, up-regulated in colorectal cancer, through inhibiting the expression of PTEN, result in B7-H1 over-expression in colorectal cancer.