126 results on '"Ruining Wang"'
Search Results
2. Genetic variation and recombination analysis of PRRSV-2 GP3 gene in China from 1996 to 2023
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Chen Lv, Yajie Zheng, Kexin Liu, Gan Li, Qin Luo, Hang Zhang, Huiyang Sha, Ruining Wang, Weili Kong, and Mengmeng Zhao
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PRRSV ,GP3 gene ,genetic variation ,genetic recombination ,phylogeny ,Microbiology ,QR1-502 - Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) has become widespread in China particularly the highly pathogenic porcine reproductive and respiratory syndromes (HP-PRRSV), NADC30, and NADC34 strains, and has posed a threat to the swine industry for over 20 years. To monitor genetic variation in PRRSV-2 GP3 strains in China, we analyzed 618 strains isolated between 1996 to 2023 and constructed phylogenetic trees. Additionally, 60 selected strains were used to analyze nucleotide and amino acid homology. PRRSV GP3 gene exhibited nucleotide identity ranging from 78.2% to 100.0% and amino acid similarity ranging from 74.9% to 99.6%. The GP3 gene in the 60 selected strains consisted of 254 amino acids, and amino acid mutations in the strains primarily occurred in B-cell epitopes, T-cell epitopes, and highly variable regions. The glycosylation sites of the strains used for amino acid sequence comparisons remained unaltered, except for the N29 site in the GD20220303-2022 strain. PRRSV-2 strains in China belong to lineages 1, 3, 5, and 8. Recombination analysis detected two recombination events, involving lineages 1 and 8. In conclusion, this study investigated multiple strains of the PRRSV-2 GP3 gene to explore the prevalence and genetic diversity of the GP3 gene in China from a gene family perspective. The results of the analyses provide a basis for clinical prevention strategies and vaccine development.
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- 2024
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3. Decomposing loss aversion from a single neural signal
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Ruining Wang, Xiaoyi Wang, Michael L. Platt, and Feng Sheng
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Biological sciences ,Neuroscience ,Cognitive neuroscience ,Science - Abstract
Summary: People often display stronger aversion to losses than appetite for equivalent gains, a widespread phenomenon known as loss aversion. The prevailing theory attributes loss aversion to a valuation bias that amplifies losses relative to gains. An alternative account attributes loss aversion to a response bias that avoids choices that might result in loss. By modeling the temporal dynamics of scalp electrical activity during decisions to accept or reject gambles within a sequential sampling framework, we decomposed valuation bias and response bias from a single event-related neural signal, the P3. Specifically, we found valuation bias manifested as larger sensitivity of P3 to losses than gains, which was localizable to reward-related brain regions. By contrast, response bias manifested as larger P3 preceding gamble acceptance than rejection and was localizable to motor cortex. Our study reveals the dissociable neural biomarkers of response bias and valuation bias underpinning loss-averse decisions.
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- 2024
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4. Research progress on the N protein of porcine reproductive and respiratory syndrome virus
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Yajie Zheng, Gan Li, Qin Luo, Huiyang Sha, Hang Zhang, Ruining Wang, Weili Kong, Jiedan Liao, and Mengmeng Zhao
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porcine reproductive and respiratory syndrome virus N protein ,genetic evolution ,protein interactions ,detection techniques ,vaccine development ,Microbiology ,QR1-502 - Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV exhibits genetic diversity and complexity in terms of immune responses, posing challenges for eradication. The nucleocapsid (N) protein of PRRSV, an alkaline phosphoprotein, is important for various biological functions. This review summarizes the structural characteristics, genetic evolution, impact on PRRSV replication and virulence, interactions between viral and host proteins, modulation of host immunity, detection techniques targeting the N protein, and progress in vaccine development. The discussion provides a theoretical foundation for understanding the pathogenic mechanisms underlying PRRSV virulence, developing diagnostic techniques, and designing effective vaccines.
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- 2024
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5. Research Progress on the NSP10 Protein of Porcine Reproductive and Respiratory Syndrome Virus
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Gan Li, Yajie Zheng, Qin Luo, Yaohua Liang, Hang Zhang, Huiyang Sha, Ruining Wang, Weili Kong, and Mengmeng Zhao
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PRRSV ,NSP10 ,helicase ,genetic evolution ,apoptosis ,viral virulence ,Biology (General) ,QH301-705.5 - Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and pathogenic infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). It manifests as reproductive disorders in sows and respiratory disorders in piglets. PRRSV infects swine herds with symptoms such as abortions, stillbirths, and mummified fetuses in gestating sows. Piglets mainly experience abdominal respiration and respiratory symptoms. To date, the prevention of PRRS relies primarily on vaccination and the implementation of various preventive and control measures. Swine deaths caused by PRRS have resulted in significant economic losses to the pig industry worldwide. Non-structural protein 10 (NSP10) has helicase and adenosine triphosphatase (ATPase) activities that unwind DNA and RNA and play important roles in viral replication and transcription. Hence, it can be potentially used to develop novel reagents for the detection of PPRSV. This article reviews genetic variations, interaction with viral and host proteins, effects on PRRSV replication, immunomodulation, apoptosis, and viral virulence of NSP10, with the aim of providing a theoretical basis for the prevention and control of PRRS and drug development in the future.
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- 2024
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6. Corrigendum: Self-assembling and pH-Responsive protein nanoparticle as potential platform for targeted tumor therapy
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Zhikun Xu, Xiaozhan Zhang, Wang Dong, Huifang lv, Lijie Zuo, Lifei Zhu, Ruining Wang, and Xia Ma
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trichosanthin ,self-assembly ,pH-Responsive ,protein nanoparticle ,tumor targeting ,Biology (General) ,QH301-705.5 - Published
- 2023
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7. Self-assembling and pH-responsive protein nanoparticle as potential platform for targeted tumor therapy
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Zhikun Xu, Xiaozhan Zhang, Wang Dong, Huifang lv, Lijie Zuo, Lifei Zhu, Ruining Wang, and Xia Ma
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trichosanthin ,self-assembly ,pH-Responsive ,protein nanoparticle ,tumor targeting ,Biology (General) ,QH301-705.5 - Abstract
Frequent injections at high concentrations are often required for many therapeutic proteins due to their short in vivo half-life, which usually leads to unsatisfactory therapeutic outcomes, adverse side effects, high cost, and poor patient compliance. Herein we report a supramolecular strategy, self-assembling and pH regulated fusion protein to extend the in vivo half-life and tumor targeting ability of a therapeutically important protein trichosanthin (TCS). TCS was genetically fused to the N-terminus of a self-assembling protein, Sup35p prion domain (Sup35), to form a fusion protein of TCS-Sup35 that self-assembled into uniform spherical TCS-Sup35 nanoparticles (TCS-Sup35 NP) rather than classic nanofibrils. Importantly, due to the pH response ability, TCS-Sup35 NP well retained the bioactivity of TCS and possessed a 21.5-fold longer in vivo half-life than native TCS in a mouse model. As a result, in a tumor-bearing mouse model, TCS-Sup35 NP exhibited significantly improved tumor accumulation and antitumor activity without detectable systemic toxicity as compared with native TCS. These findings suggest that self-assembling and pH responding protein fusion may provide a new, simple, general, and effective solution to remarkably improve the pharmacological performance of therapeutic proteins with short circulation half-lives.
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- 2023
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8. Identification of the Linear Fc-Binding Site on the Bovine IgG1 Fc Receptor (boFcγRIII) Using Synthetic Peptides
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Ruining Wang, Junqing Guo, Ge Li, Xun Wang, Jifei Yang, Qingmei Li, and Gaiping Zhang
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BoFcγRIII ,Fc-binding site ,bovine IgG1 ,synthetic peptides ,Veterinary medicine ,SF600-1100 - Abstract
The bovine IgG1 Fc receptor (boFcγRIII) is a homologue to human FcγRIII (CD16) that binds bovine IgGI with medium–low affinity. In order to identify the Fc-binding site on the bovine IgG1 Fc receptor (boFcγRIII), peptides derived from the second extracellular domain (EC2) of boFcγRIII were synthesized and conjugated with the carrier protein. With a Dot-blot assay, the ability of the peptides to bind bovine IgG1 was determined, and the IgG1-binding peptide was also identified via truncation and mutation. The minimal peptide AQRVVN corresponding to the sequence 98–103 of boFcγRIII bound bovine IgG1 in Dot-blot, suggesting that it represents a linear ligand-binding site located in the putative A–B loop of the boFcγRIII EC2 domain. Mutation analysis of the peptide showed that the residues of Ala98, Gln99, Val101, Val102 and Asn103 within the Fc-binding site are critical for IgG1 binding on boFcγRIII. The functional peptide identified in this paper is of great value to the IgG–Fc interaction study and FcR-targeting drug development.
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- 2024
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9. TRIM4-mediated ubiquitination of NSP2 restricts porcine reproductive and respiratory syndrome virus proliferation
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Mengmeng Zhao, Huiyang Sha, Hang Zhang, and Ruining Wang
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PRRSV ,NSP2 ,Ubiquitination ,Degradation ,TRIM4 ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and virulent infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV), which has substantial economic losses in the pig industry worldwide, and PRRSV attenuated vaccines and inactivated vaccines do have limitations in immune protection. The discovery of new antiviral targets has become a new research field. The proteomic studies have shown that the PRRSV NSP2 protein interacts with tripartite motif protein 4 (TRIM4), but it was still unknown whether TRIM4 regulates PRRSV infections. In this study, the TRIM4 gene from Marc-145 cells was cloned, and it was proved that TRIM4 overexpression inhibits PRRSV replication, whereas TRIM4 small-interfering-RNA knockdown resulted in increased virus titers. Mechanism investigation indicated that TRIM4 inhibits PRRSV replication through ubiquitination and degradation of the NSP2 protein. Protease inhibitor MG132 (carbobenzoxy-Leu-Leu-leucinal) attenuated the TRIM4-driven degradation of NSP2. Taken together, TRIM4 impairs PRRSV proliferation via ubiquitination and degradation of NSP2.
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- 2022
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10. Coronavirus stress and overeating: the role of anxiety and COVID-19 burnout
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Ruining Wang, Baojuan Ye, Peiyi Wang, Chunyan Tang, and Qiang Yang
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Coronavirus stress ,Anxiety ,Overeating ,COVID-burnout ,College students ,COVID-19 ,Psychiatry ,RC435-571 - Abstract
Plain English summary One’s response is not arbitrary when confronted with the coronavirus disease 2019 (COVID-19). Previous research has found that individuals with coronavirus stress still be more prone to overeat. We also know from other research that anxiety is the most salient aspect of overeating. However, no research has investigated whether the coronavirus stress of college students is significantly associated with overeating and examine the potential indirect pathway and moderating mechanisms in this association. With the aid of 2926 participants, we found that, coronavirus stress was linked to college students’ overeating. We also found that this relationship was partially explained by anxiety. In addition, the association between anxiety and overeating was stronger for those with higher COVID-19 burnout. This study is an important step in unpacking how coronavirus stress relates to overeating of Chinese college students. However, they are limited by the cross-sectional nature of the study, meaning we cannot imply causality. We recommend that further research replicate our findings in people with diagnosed feeding and eating disorders using a longitudinal design.
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- 2022
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11. Metabolic Characteristics of Different Carbon Sources by Microorganisms in Sediment of High-density Tilapia Pond
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Miao WANG, Mengmeng YI, Ruining WANG, and Maixin LU
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tilapia ,pond ,sediment ,biolog-eco plate ,carbon and nitrogen metabolism ,microbial community ,Agriculture - Abstract
【Objective】Microbial community in sediment plays an important role in maintaining the balance of pond ecosystem. The present research aimed to explore the characteristics and seasonal variation of carbon and nitrogen metabolism of microorganisms in sediment of high-density tilapia pond.【Method】Sediment samples were collected during the aquaculture season, and the contents of nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total nitrogen in sediment were analyzed. The average well color development (AWCD) of microbial metabolism of substrates containing both C and N, containing both C and P and containing C only was analyzed by Biolog-ECO plates.【Result】The contents of total nitrogen and ammonia nitrogen in pond sediment in July and October were significantly higher than those in April. The microorganisms in the sediment could use substrates containing C only, including β-methyl-D-glucoside, pyruvic acid methyl ester, D-galacturonic acid, tween 80, D-mannitol and D-cellobiose, substrates containing C and N, including L-asparagine and N-acetyl-D-glucosamine (AWCD > 0.5), and substrates containing C and P, including D, L-α-glycerol phosphate and glucose-1-phosphate (AWCD < 0.5). 2-hydroxy benzoic acid, itaconic acid and α-ketobutyric acid could not be utilized. In April, AWCD of substrates containing C and N and substrates containing C only was higher than those in July and October. AWCD of substrates containing C and N, and substrates containing C only was positively correlated with nitrate content in sediment.【Conclusion】Biolog-ECO plate technology could be used to analyze nitrogen use index of sediment microorganisms for evaluating nitrogen removal capacity of microbial community in sediment.
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- 2022
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12. Life history strategy and overeating during COVID-19 pandemic: a moderated mediation model of sense of control and coronavirus stress
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Baojuan Ye, Ruining Wang, Mingfan Liu, Xinqiang Wang, and Qiang Yang
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Life history strategy ,Sense of control ,Coronavirus stress ,Overeating ,College students ,COVID-19 ,Psychiatry ,RC435-571 - Abstract
Plain English summary One’s response is not arbitrary when confronted with COVID-19 pandemic. Previous research has found that individuals with fast life history strategies still be more prone to overeat. We also know from other research that sense of control is the most salient aspect of overeating. However, no research has investigated whether the life history strategy of college students is significantly associated with overeating and examine the potential mediating and moderating mechanisms in this association. With the aid of 3310 participants, we found that, life history strategy was linked to college students’ overeating. We also found that this relationship was partially explained by sense of control. In addition, the association between life history strategy and sense of control was stronger for those with lower coronavirus stress, and the association between sense of control and overeating was stronger for those with lower coronavirus stress. This study is an important step in unpacking how life history strategy relates to overeating in Chinese college students. However, they are limited by the cross-sectional nature of the study, meaning we cannot imply causality. We recommend that further research replicate our findings in people with diagnosed eating disorders using a longitudinal design.
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- 2021
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13. Corrigendum: The effects of empathy by caregivers on healthcare service satisfaction
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Xiaoyi Wang, Ruining Wang, Feng Sheng, and Leyi Chen
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service satisfaction ,physician–patient communication ,empathy ,patient-centered care ,patient autonomy ,electroencephalography (EEG) ,Psychology ,BF1-990 - Published
- 2022
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14. Differentiation of Classical Swine Fever Virus Virulent and Vaccine Strains by CRISPR/Cas13a
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Yuhang Zhang, Qingmei Li, Ruining Wang, Li Wang, Xun Wang, Jun Luo, Guangxu Xing, Guanmin Zheng, Bo Wan, Junqing Guo, and Gaiping Zhang
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classical swine fever virus ,recombinase-aided amplification ,CRISPR/Cas13a ,differentiation of infected and vaccinated animals ,Microbiology ,QR1-502 - Abstract
ABSTRACT As a notifiable terrestrial and aquatic animal disease listed by World Organisation for Animal Health (formerly the Office International des Epizooties [OIE]), classical swine fever (CSF) has caused great economic losses to the swine industry worldwide during recent decades. Differentiation of infected and vaccinated animals (DIVA) is urgent for eradication of CSF. In this study, a diagnostic platform based on CRISPR/Cas13a was established with the ability to differentiate between classical swine fever virus (CSFV) virulent and vaccine strains. In combination with reverse transcription recombinase-aided amplification (RT-RAA), the detection limit for CSFV synthetic RNA templates reached 3.0 × 102 copies/μL. In addition, with boiling and chemical reduction, heating unextracted diagnostic samples to obliterate nucleases (HUDSON) treatment was introduced to inactivate nucleases and release viral genome, achieving robust pretreatment of tested sample before CRISPR/Cas13a detection without the need to extract viral nucleic acids. HUDSON-RT-RAA-CRISPR/Cas13a can directly detect cell cultures of virulent Shimen strain and vaccine hog cholera lapinized virus (HCLV) strain, with the detection limit of 3.5 × 102 copies/μL and 1.8 × 102 copies/μL, respectively, which was equally sensitive to nested PCR (nPCR) and 100 times more sensitive than antigen enzyme-linked immunosorbent assay (ELISA). Meanwhile, HUDSON-RT-RAA-CRISPR/Cas13a showed no cross-reactivity with bovine viral diarrhea virus (BVDV), atypical porcine pestivirus (APPV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), African swine fever virus (ASFV), pseudorabies virus (PRV), and porcine circovirus 2 (PCV2), exhibiting good specificity. At last, a total of 50 pig spleen samples with suspected clinical signs were also assayed with HUDSON-RT-RAA-CRISPR/Cas13a, nPCR, and antigen ELISA in parallel. HUDSON-RT-RAA-CRISPR/Cas13a showed 100.0% with nPCR and 82.0% coincident rate with antigen ELISA, respectively. IMPORTANCE Classical swine fever (CSF) is a World Organisation for Animal Health (formerly the Office International des Epizooties [OIE]) notifiable terrestrial and aquatic animal disease, causing great economic losses to the swine industry worldwide during the past decades. Due to the use of the most effective and safe attenuated live vaccine for CSF prevention, differentiation of infected and vaccinated pigs is vital work, as well as a bottleneck for eradication of CSF. Methods with the ability to precisely differentiate classical swine fever virus (CSFV) virulent strains from vaccine strain hog cholera lapinized virus (HCLV) are urgently needed. Combining the high sensitivity of isothermal recombinase-aided amplification (RAA) with the accurate molecular sensing ability of Cas13a, we presented a novel method for CSFV detection without the need to extract viral nucleic acids, which showed great advantage to traditional detection methods for precise differentiation of CSFV virulent strains and vaccine strain, providing a novel powerful tool for CSF eradication.
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- 2022
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15. The effects of empathy by caregivers on healthcare service satisfaction
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Xiaoyi Wang, Ruining Wang, Feng Sheng, and Leyi Chen
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service satisfaction ,physician–patient communication ,empathy ,patient-centered care ,patient autonomy ,electroencephalography (EEG) ,Psychology ,BF1-990 - Abstract
Healthcare service satisfaction focuses not only on the patients but also on the caregivers’ perspectives. This study explored how caregivers’ empathy toward patients affects their satisfaction with healthcare services through an electroencephalography (EEG) experiment. EEG mu rhythm was used as the neural indicator to reflect empathy. The results showed that empathy reduces caregivers’ evaluation of healthcare service satisfaction because they share suffering with the patients. However, implementing physician–patient communication through a process-based informed consent (IC), compared to an event-based IC, can effectively alleviate such adverse effects.
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- 2022
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16. Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system
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Miao Wang, Zijian Fan, Ruining Wang, Zhigang Liu, Fengying Gao, Ziyue Zhang, Mengmeng Yi, and Maixin Lu
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Nitrogen removal ,Microbial community ,Nitrogen metabolism ,Ecological engineering pond aquaculture system ,Biolog EcoPlates ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
The ecological engineering pond aquaculture system was promoted in China in recent years as it can save water and reduce pollution. In the present study, an ecological engineering pond aquaculture system was established, consisting of a drainage ditch, a settling pond, an aeration pond, a filter dam, eco-ponds, and fish ponds for tilapia farming. Water from different ponds were sampled and sequenced by Illumina sequencing, and then the microbial communities were analyzed. The metabolic activity of bacteria in the water samples was tested using Biolog EcoPlates. The concentrations of total nitrogen (TN), ammonia-nitrogen (NH4-N), nitrate-nitrogen (NO3-N), nitrite-nitrogen (NO2-N), and total phosphorus (TP) in water after treatment by the system were 0.71 ± 0.19, 0.48 ± 0.15, 0.51 ± 0.21, 0.03 ± 0.01, and 0.04 ± 0.01 mg/L, respectively. All the units of the system could remove TN in water. Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, and Planctomycetes were the dominant phyla in the water of the system. The genera Candidatus Aquiluna and Dechloromonas positively correlated with the microbial metabolic activity of organic nitrogen and NH4-N concentrations in water, which could be the functional bacteria for nitrogen removal. Several genera showed positive correlation with the TN and NH4-N concentrations of water and were probably involved in nitrogen cycling of the system. Our data indicated that the ecological engineering pond aquaculture system notably improves water quality and alters the microbial populations. The results supply substantial information on the functional microbiota for nitrogen removal from freshwater aquaculture ecosystems.
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- 2022
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17. The Impact of Differentiated Carbon Taxes on New Enterprises’ Strategies When Entering Original Markets with Different Degrees of Market Competition
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Jialin Zheng, Ya Zhou, Keqiang Li, Yang Zeng, Ruining Wang, and Canmin Zhang
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market competition ,low-carbon production ,new entrants ,original market ,game ,carbon tax ,Mathematics ,QA1-939 - Abstract
We view the development of industries with various market competition levels as a dynamic process and investigate the game between a new entrant and the original market with variable market competition degrees under the premise of considering the entry and exit of companies in the industry. Based on this, we explore the prerequisites for the new firm to enter the initial market and construct a recursive formula for the optimal output of individual firms entering the market one at a time, as well as the conditions for the new firm to enter the market in the three scenarios of the original market being mixed strategy, low-carbon type, and traditional type, respectively, and the optimal decision-making behavior once entering the market. We create diversified carbon tax rates for various cost bands of low-carbon production patterns in order to modify the original traditional market and allow the new enterprise to enter the market using a low-carbon production strategy. We anticipate that our study will serve as a theoretical guide for accomplishing a low-carbon shift in production patterns.
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- 2023
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18. Sequence Analysis of Macaca mulatta TRIM4 and Its Role in the Interferon Pathway
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Mengmeng Zhao, Huawei Li, Hang Zhang, Huiyang Sha, Liangzong Huang, and Ruining Wang
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TRIM4 ,interferon ,innate immunity ,Macaca mulatta ,CCL5 ,Veterinary medicine ,SF600-1100 - Abstract
Monkey diseases are becoming increasingly severe, and some may be transmitted to humans through direct and indirect contact. Innate immunity is the first line of defense against foreign microorganisms. It is of great significance to explore the immune characteristics of monkey and human diseases. TRIM4, an important immune molecule in Macaca mulatta, was cloned and its immunological characteristics were preliminarily explored. The results showed that Macaca mulatta TRIM4 was in the same branch as human TRIM4. Overexpression of TRIM4 increased the mRNA levels of interferon (IFN)-alpha, IFN-beta, RIG-I, MAVS, IRF3, IRF7, OAS1, IFIT3, and CCL5, TRIM4 up-regulated the activities of IFN-beta, NF-κB, and ISRE reporter. In contrast, inhibiting TRIM4 expression by small interfering RNA (siRNA) down-regulated the IFN pathway. In summary, Macaca mulatta TRIM4 plays an essential role in the IFN pathway.
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- 2022
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19. Natural Compound 2,2′,4′-Trihydroxychalcone Suppresses T Helper 17 Cell Differentiation and Disease Progression by Inhibiting Retinoid-Related Orphan Receptor Gamma T
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Yana Yang, Wenhui Qi, Yanyan Zhang, Ruining Wang, Mingyue Bao, Mengyuan Tian, Xing Li, and Yuan Zhang
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chalcone derivant ,RORγt ,Th17 cell differentiation ,experimental autoimmune encephalomyeliti ,experimental colitis ,skin allograft rejection ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Retinoid-related orphan receptor γt (RORγt), a vital transcription factor for the differentiation of the pro-inflammatory Th17 cells, is essential to the inflammatory response and pathological process mediated by Th17 cells. Pharmacological inhibition of the nuclear receptor RORγt provides novel immunomodulators for treating Th17-driven autoimmune diseases and organ transplant rejection. Here, we identified 2,2′,4′-trihydroxychalcone (TDC), a natural chalcone derivant, binds directly to the ligand binding domain (LBD) of RORγt and inhibited its transcriptional activation activity. Using three mice models of Th17-related diseases, it was found that the administration of TDC effectively alleviated the disease development of experimental autoimmune encephalomyelitis (EAE), experimental colitis, and skin allograft rejection. Collectively, these results demonstrated TDC targeting RORγt to suppress Th17 cell polarization, as well as its activity, thus, indicating the potential of this compound in treating of Th17-related autoimmune disorders and organ transplant rejection disorders.
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- 2022
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20. Attentional and emotional brain response to message framing in context of green marketing
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Muhammad Zubair, Xiaoyi Wang, Sidra Iqbal, Muhammad Awais, and Ruining Wang
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Attention ,Emotion ,Event-related brain potentials ,Green marketing ,Message framing ,ERPs ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Message framing plays an important role in advertising strategies and has been studied from various perspectives in different behavioral studies. New method: This study employs the event-related potential technique to examine attentional and emotional brain processing as influenced by message framing in the context of green marketing. Results: The behavioral results demonstrated that purchase preference was higher under positive framing compared to negative and neutral framing.As per the event-related potential results, negative framing elicited a larger P1 component, which reveals that in the first stage of processing information, threatening information attracted more attention. In the second and third stage, N170 and P3, respectively, were higher for positive framing, demonstrating that there was more attention toward the processing of non-threatening emotional information.Comparison with existing method: Message Framing has been previously examined with behavioral methods. We for the first time examined it with a neuroscientific method like Event Related Brain Potential technique in a green marketing context. Conclusion: Our results compared to behavioral studies provide stronger evidence from underlying neural perspective for how message framing can be affected by attentional and emotional brain responses in the context of green marketing.
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- 2020
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21. Spin-wave modes of elliptical skyrmions in magnetic nanodots
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Chendong Jin, Shuang Li, Hu Zhang, Ruining Wang, Jianglong Wang, Ruqian Lian, Penglai Gong, and Xingqiang Shi
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elliptical skyrmion ,spin-wave mode ,anisotropic DMI ,Science ,Physics ,QC1-999 - Abstract
Magnetic skyrmions, whose shapes are ellipse due to the presence of anisotropic Dzyaloshinskii–Moriya interaction (DMI), have already been discovered in experiments recently. By using micromagnetic simulations, we discuss the ground state and the spin-wave modes of a single elliptical skyrmion in a confined nanodot. It is found that the shapes of skyrmion are stretched into a horizontal ellipse, vertical ellipse, or stripe shape under different strengths of anisotropic DMI. When elliptical skyrmions are excited by in-plane ac magnetic fields, the spin-wave mode contains a counterclockwise rotation mode at high frequencies and a clockwise (CW) rotation mode at low frequencies, and the CW mode depends on the strength of anisotropic DMI. When elliptical skyrmions are excited by out-of-plane ac magnetic fields, the spin-wave mode is split from a simple breathing mode into two complex breathing modes, including a mixed mode of CW rotation and breathing, and another anisotropic breathing mode. Our results provide an understanding of the rich spin-wave modes for skyrmions, which may contribute to the applications in magnonics.
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- 2022
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22. Spin-wave modes of magnetic bimerons in nanodots
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Chendong Jin, Shuang Li, Hu Zhang, Ruining Wang, Jianglong Wang, Ruqian Lian, Penglai Gong, and Xingqiang Shi
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bimerons ,in-plane anisotropy ,spin-wave mode ,Science ,Physics ,QC1-999 - Abstract
We report the resonance excitations and the spin-wave modes of a single bimeron in a confined nanodot by using micromagnetic simulations. Magnetic bimerons can be considered as in-plane topological spin textures of magnetic skyrmions, which means that the spin-wave modes of bimerons also rotate in-plane compared to skyrmions, for example, through the application of out-of-plane microwave magnetic fields, the spin-wave mode of bimerons is no longer a breathing mode but contains a counterclockwise mode at low frequencies and a clockwise mode at high frequencies. When in-plane microwave magnetic fields rotated at different angles are applied, the spin-wave mode of bimerons has an anisotropic property, i.e., the spin-wave mode presents as a breathing mode for the microwave magnetic field applied along the x -direction, and a couple of azimuthal modes for the microwave magnetic field applied along the y -direction. Moreover, we demonstrate that the breathing mode, the counterclockwise rotation mode, and the clockwise rotation mode can simultaneously appear together when the microwave magnetic field is applied at a specific angle in the plane. In addition to the three typical spin-wave modes, two high-phase counterclockwise rotation modes lead to the periodic deformation of bimerons due to the broken rotational symmetry of the spin texture. Our results reveal the rich spin-wave modes of bimerons, which may contribute to the applications in spintronics and magnonics.
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- 2022
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23. Glucocorticoids Suppress Antimicrobial Autophagy and Nitric Oxide Production and Facilitate Mycobacterial Survival in Macrophages
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Jinli Wang, Ruining Wang, Hui Wang, Xiaofan Yang, Jiahui Yang, Wenjing Xiong, Qian Wen, and Li Ma
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Medicine ,Science - Abstract
Abstract Chronic administration of glucocorticoids has been shown to render individuals highly susceptible to mycobacterial infection and lead to reactivation of latent bacilli. However, the effect of glucocorticoids on innate anti-mycobacterial defense, especially in macrophages remains largely unknown. Here, we found that glucocorticoids inhibited the innate immune response, antimicrobial nitric oxide production and autophagy in mycobacteria-challenged macrophages. Meanwhile, maturation and acidification of mycobacterial phagosomes were attenuated in RAW264.7 cells after glucocorticoids treatment. Consequently, we observed a glucocorticoid-induced increase in the survival of intracellular mycobacteria in both primary macrophages and cell lines. Glucocorticoids treatment decreased the activation of TBK1 kinase, which promotes the maturation of autophagosomes. Inhibition of TBK1 also decreased the production of nitric oxide. Furthermore, several autophagy-related genes were down-regulated, while activation of the Akt/mTOR signaling pathway was increased after glucocorticoids treatment, which may account for autophagy inhibition during mycobacterial infection. Restoration of autophagy with the agonist rapamycin abolished glucocorticoid-mediated enhancement of mycobacterial survival, suggesting that glucocorticoids blocked anti-mycobacterial defense via autophagy inhibition. Collectively, this study demonstrates that glucocorticoids impair innate antimicrobial autophagy and promote mycobacterial survival in macrophages, which is a novel mechanism for glucocorticoid-mediated immunosuppression. Our findings may provide important clues for tuberculosis prevention.
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- 2017
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24. NLRC3 negatively regulates CD4+ T cells and impacts protective immunity during Mycobacterium tuberculosis infection.
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Shengfeng Hu, Xialin Du, Yulan Huang, Yuling Fu, Yalong Yang, Xiaoxia Zhan, Wenting He, Qian Wen, Xinying Zhou, Chaoying Zhou, Xiao-Ping Zhong, Jiahui Yang, Wenjing Xiong, Ruining Wang, Yuchi Gao, and Li Ma
- Subjects
Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
NLRC3, a member of the NLR family, has been reported as a negative regulator of inflammatory signaling pathways in innate immune cells. However, the direct role of NLRC3 in modulation of CD4+ T-cell responses in infectious diseases has not been studied. In the present study, we showed that NLRC3 plays an intrinsic role by suppressing the CD4+ T cell phenotype in lung and spleen, including differentiation, activation, and proliferation. NLRC3 deficiency in CD4+ T cells enhanced the protective immune response against Mycobacterium tuberculosis infection. Finally, we demonstrated that NLRC3 deficiency promoted the activation, proliferation, and cytokine production of CD4+ T cells via negatively regulating the NF-κB and MEK-ERK signaling pathways. This study reveals a critical role of NLRC3 as a direct regulator of the adaptive immune response and its protective effects on immunity during M. tuberculosis infection. Our findings also suggested that NLRC3 serves as a potential target for therapeutic intervention against tuberculosis.
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- 2018
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25. Vitamin B5 Reduces Bacterial Growth via Regulating Innate Immunity and Adaptive Immunity in Mice Infected with Mycobacterium tuberculosis
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Wenting He, Shengfeng Hu, Xialin Du, Qian Wen, Xiao-Ping Zhong, Xinying Zhou, Chaoying Zhou, Wenjing Xiong, Yuchi Gao, Shimeng Zhang, Ruining Wang, Jiahui Yang, and Li Ma
- Subjects
tuberculosis ,vitamin B5 ,pro-inflammation ,antibacteria ,macrophages ,immune cells ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The mechanisms by which vitamins regulate immunity and their effect as an adjuvant treatment for tuberculosis have gradually become very important research topics. Studies have found that vitamin B5 (VB5) can promote epithelial cells to express inflammatory cytokines. We aimed to examine the proinflammatory and antibacterial effect of VB5 in macrophages infected with Mycobacterium tuberculosis (MTB) strain H37Rv and the therapeutic potential of VB5 in vivo with tuberculosis. We investigated the activation of inflammatory signal molecules (NF-κB, AKT, JNK, ERK, and p38), the expression of two primary inflammatory cytokines (tumor necrosis factor and interleukin-6) and the bacterial burdens in H37Rv-infected macrophages stimulated with VB5 to explore the effect of VB5 on the inflammatory and antibacterial responses of macrophages. We further treated the H37Rv-infected mice with VB5 to explore VB5’s promotion of the clearance of H37Rv in the lungs and the effect of VB5 on regulating the percentage of inflammatory cells. Our data showed that VB5 enhanced the phagocytosis and inflammatory response in macrophages infected with H37Rv. Oral administration of VB5 decreased the number of colony-forming units of H37Rv in lungs of mice at 1, 2, and 4 weeks after infection. In addition, VB5 regulated the percentage of macrophages and promoted CD4+ T cells to express interferon-γ and interleukin-17; however, it had no effect on the percentage of polymorphonuclear neutrophils, CD4+ and CD8+ T cells. In conclusion, VB5 significantly inhibits the growth of MTB by regulating innate immunity and adaptive immunity.
- Published
- 2018
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26. Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography
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Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG
- Subjects
classical swine fever virus ,virus purification ,tangential-flow filtration ,size-exclusion chromatography ,Agriculture (General) ,S1-972 - Abstract
Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium. Hence, we have developed an efficient method for purifying CSFV from cell-culture medium. Pure viral particles were obtained with two steps of tangential-flow filtration (TFF) and size-exclusion chromatography (SEC), and were compared with particles from ultracentrifugation by transmission electron microscopy (TEM), infectivity and recovery test, and real time fluorescent quantitative PCR (FQ-PCR). TFF concentrated the virus particles effectively with a retention rate of 98.5%, and 86.2% of viral particles were obtained from the ultrafiltration retentate through a Sepharose 4 F F column on a biological liquid chromatography system. CSFV purified by TFF-SEC or ultracentrifugation were both biologically active from 1.0×10-4.25 TCID50·mL-1 to 3.0×10-6.25 TCID50·mL-1, but the combination of TFF and SEC produced more pure virus particles than by ultracentrifugation alone. In addition, pure CSFV particles with the expected diameter of 40—60 nm were roughly spherical without any visible contamination. Mice immunized with CSFV purified by TFF-SEC produced higher antibody levels compared with immunization with ultracentrifugation-purified CSFV (P
- Published
- 2015
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- View/download PDF
27. Improved structural and electrical properties in native Sb2Te3/GexSb2Te3+x van der Waals superlattices due to intermixing mitigation
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Stefano Cecchi, Eugenio Zallo, Jamo Momand, Ruining Wang, Bart J. Kooi, Marcel A. Verheijen, and Raffaella Calarco
- Subjects
Biotechnology ,TP248.13-248.65 ,Physics ,QC1-999 - Abstract
Superlattices made of Sb2Te3/GeTe phase change materials have demonstrated outstanding performance with respect to GeSbTe alloys in memory applications. Recently, epitaxial Sb2Te3/GeTe superlattices were found to feature GexSb2Te3+x blocks as a result of intermixing between constituting layers. Here we present the epitaxy and characterization of Sb2Te3/GexSb2Te3+x van der Waals superlattices, where GexSb2Te3+x was intentionally fabricated. X-ray diffraction, Raman spectroscopy, scanning transmission electron microscopy, and lateral electrical transport data are reported. The intrinsic 2D nature of both sublayers is found to mitigate the intermixing in the structures, significantly improving the interface sharpness and ultimately the superlattice structural and electrical properties.
- Published
- 2017
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28. The Multiplicity of Infection-Dependent Effects of Recombinant Adenovirus Carrying HGF Gene on the Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells
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Qian Wen, Shimeng Zhang, Xialin Du, Ruining Wang, Yanfen Li, Honglin Liu, Shengfeng Hu, Chaoying Zhou, Xinying Zhou, and Li Ma
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avascular necrosis of femoral head ,human bone marrow mesenchymal stem cell ,hepatocyte growth factor ,adenovirus ,signaling pathway ,proliferation ,osteogenesis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Absence of effective therapeutic methods for avascular necrosis of femoral head (ANFH) is still perplexing the world’s medical community. Bone marrow mesenchymal stem cells (BMSCs) adoptive cell therapy combined with core decompression is a promising modality, which is highly dependent on the cellular activities of BMSCs. Hepatocyte growth factor (HGF) is a survival factor for BMSCs, yet the underlying mechanism is not fully elucidated. In this study, the effects of multiplicity of infections (MOIs) of recombinant adenovirus carrying HGF gene (rAd-HGF) on human BMSC proliferation and osteogenic differentiation were systemically examined. Infection of rAd-HGF produced secretory HGF and promoted hBMSC proliferation in a MOI-dependent manner, while the osteogenesis was also strengthened as indicated by enhanced calcium nodule formation with the strongest effects achieved at MOI = 250. Blocking the activities of c-MET or its downstream signaling pathways, WNT, ERK1/2, and PI3K/AKT led to differential consequents. Specifically, blockage of the WNT pathway significantly promoted osteogenic differentiation, which also showed additive effects when combined application with rAd-HGF. Our data demonstrated the pro-osteogenic effects of optimized MOIs of rAd-HGF, while inhibition of WNT pathway or activation of PI3K/AKT pathway may act as candidate adjuvant modalities for promoting osteogenic differentiation in rAd-HGF-modified hBMSC treatment on ANFH.
- Published
- 2018
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29. TIENet: task-oriented image enhancement network for degraded object detection.
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Yudong Wang, Jichang Guo, Ruining Wang, Wanru He, and Chongyi Li
- Published
- 2024
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30. Real-time Semantic Segmentation in Traffic Scene based on Cross Stage Partial Block.
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Lian Liu, Liguo Zhou, Zhenshan Bing, Ruining Wang, and Alois Knoll
- Published
- 2022
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31. On Adversarial Robustness of Semantic Segmentation Models for Automated Driving.
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Huilin Yin, Ruining Wang, Boyu Liu, and Jun Yan
- Published
- 2022
- Full Text
- View/download PDF
32. Attention Mechanism for Contrastive Learning in GAN-based Image-to-Image Translation.
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Hanzhen Zhang, Liguo Zhou, Ruining Wang, and Alois C. Knoll
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- 2023
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33. Real-time semantic segmentation in traffic scene using Cross Stage Partial-based encoder-decoder network.
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Liguo Zhou, Guang Chen 0001, Lian Liu, Ruining Wang, and Alois Knoll
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- 2023
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34. Molecular dynamics simulation of typical molecular ferroelectrics based on polarized crystal charge model
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Ruining Wang, Feng Xu, Xiongfei Gui, and Yongle Li
- Subjects
Physical and Theoretical Chemistry - Abstract
Molecular ferroelectrics are a promising class of ferro-electrics, with environmental friendliness, flexibility and low cost. In this work, a set of characteristic molecular ferroelectrics are simulated by molecular dynamics (MD) with polarized crystal charge (PCC). From the simulated results, their ferroelectric switching mechanisms are elucidated, with their ferroelectric hysteresis loops. The PCC charge model, recently developed by our group, containing the quantum electric polarization effect, is suitable in nature for studying molecular ferroelectrics. The simulated systems include the typical molecular ionic ferroelectrics, di-isopropyl-ammonium halide (DIPAX, X=C (Cl), B (Br), and I), as well as a pair of newly validated organic molecular ferroelectrics, salicylideneaniline and (-)-camphanic acid. In total, there are five systems under investigation. Results demonstrate that the PCC MD method is efficient and reliable. It not only elucidates the ferroelectric switching mechanism of the studied molecular ferroelectrics, but also extends the application range of the PCC MD. In conclusion, PCC MD provides an efficient protocol for extensive computer simulations of molecular ferroelectrics, with reliable ferroelectric properties and associated mechanisms, and would promote further exploration of novel molecular ferroelectrics.
- Published
- 2023
35. Inverse design and high-throughput screening of TM-A (TM: Transition metal; A: O, S, Se) cathodes for chloride-ion batteries
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Mengqi Wu, Mingxiao Ma, Jianglong Wang, Ruining Wang, Xingqiang Shi, Hu Zhang, Chendong Jin, Yingjin Wei, and Ruqian Lian
- Subjects
Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,General Materials Science - Published
- 2022
36. In-plane spin excitation of skyrmion bags
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Shuang Li, Kexin Li, Zhaohua Liu, Qiyuan Zhu, Chenbo Zhao, Hu Zhang, Xingqiang Shi, Jianglong Wang, Ruining Wang, Ruqian Lian, Penglai Gong, and Chendong Jin
- Subjects
General Physics and Astronomy - Abstract
Skyrmion bags are spin structures with arbitrary topological charges, which consist of a large skyrmion and multiple small skyrmions. In this work, by applying in-plane ac magnetic fields, we investigate the spin-wave modes of skyrmion bags, which behave differently from the clockwise (CW) and counterclockwise (CCW) rotation modes of skyrmions because of their complex spin topological structure. The in-plane excitation power spectral density shows that there are four resonance frequencies for each skyrmion bag. By further calculating the spin dynamics of a skyrmion bag at each resonant frequency, the four spin-wave modes appear as the composition modes of outer skyrmion-inner skyrmions, i.e., a CCW-CW mode, two CW-breathing modes with different resonance strength, and an inner CCW mode. Our results provide an understanding of the in-plane spin excitation of skyrmion bags, which may contribute to the characterization and detection of skyrmion bags, as well as the applications in logic devices.
- Published
- 2023
37. The art of the deal: Deciphering the endowment effect from traders' eyes.
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Feng Sheng, Ruining Wang, Zexian Liang, Xiaoyi Wang, and Platt, Michael L.
- Subjects
- *
ENDOWMENTS , *LOTTERY tickets - Abstract
The article focuses on understanding the endowment effect in trading, where people are reluctant to part with what they have. It explores two hypotheses: valuation-related bias (overvaluing possessions) and response-related bias (reluctance to change the status quo). It further uses experiments and physiological measures like gaze and pupil dilation to dissect these biases in trading decisions.
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- 2023
- Full Text
- View/download PDF
38. Effect of structure on dynamical stab-resistance behaviors of laminates composite
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Ruining Wang, Runjun Sun, and Qiushi Wang
- Subjects
Renewable Energy, Sustainability and the Environment - Abstract
The dynamic stab-resistance behaviors of laminate composites composed of TA15, TA1 titanium with various thicknesses of ultra-high-molecular-weight polyethylene, and polyester fabrics are characterized under dynamic stab testing conditions and analyzed by a new method using the average velocity of tension-compaction-plastic deformation process to calculate the ratio of energy in different damage process. The damaged area is observed by optical microscopy and SEM, and the impact force-time curve and damage degree are analyzed, and the energy of different processes during the dynamic stab test is calculated. The results show that laminates with the structure of high strength material among the low strength materials have much better performance than other specimens.
- Published
- 2022
39. Life history strategy and overeating during COVID-19 pandemic: a moderated mediation model of sense of control and coronavirus stress
- Author
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Mingfan Liu, Baojuan Ye, Ruining Wang, Xinqiang Wang, and Qiang Yang
- Subjects
Psychiatry ,Nutrition and Dietetics ,Coronavirus disease 2019 (COVID-19) ,RC435-571 ,COVID-19 ,Sense of control ,Life history strategy ,medicine.disease_cause ,Life history theory ,Developmental psychology ,Behavioral Neuroscience ,Psychiatry and Mental health ,Moderated mediation ,Overeating ,Coronavirus stress ,Pandemic ,Stress (linguistics) ,medicine ,College students ,Psychology ,Research Article ,Coronavirus - Abstract
Background This study examined the mediating effect of sense of control and the moderating effect of coronavirus stress on the relationship between life history strategy and overeating among Chinese college students during the COVID-19 period. Methods 3310 Chinese students (Mage = 19.74, SD = 1.50; 47% males) completed self-reported questionnaires regarding life history strategy, sense of control, overeating, and coronavirus stress. The data were analyzed using Pearson’s r correlations and moderated mediation analysis. Results The results revealed that control sense mediated the link between life history strategy and college students’ overeating. College students’ coronavirus stress moderated the associations between life history strategy and college students’ sense of control and between control sense and overeating. The association between life history strategy and sense of control was stronger for those with lower coronavirus stress, and the association between sense of control and overeating was stronger for those with lower coronavirus stress. Conclusions This study identified that the critical factors were associated with overeating. On the one hand, the research deepens the application and interpretation of life history theory in the field of eating; on the other hand, it provides evidence for the prevention of overeating, and provides theoretical basis for psychological assistance among Chinese college students., Plain English summary One’s response is not arbitrary when confronted with COVID-19 pandemic. Previous research has found that individuals with fast life history strategies still be more prone to overeat. We also know from other research that sense of control is the most salient aspect of overeating. However, no research has investigated whether the life history strategy of college students is significantly associated with overeating and examine the potential mediating and moderating mechanisms in this association. With the aid of 3310 participants, we found that, life history strategy was linked to college students’ overeating. We also found that this relationship was partially explained by sense of control. In addition, the association between life history strategy and sense of control was stronger for those with lower coronavirus stress, and the association between sense of control and overeating was stronger for those with lower coronavirus stress. This study is an important step in unpacking how life history strategy relates to overeating in Chinese college students. However, they are limited by the cross-sectional nature of the study, meaning we cannot imply causality. We recommend that further research replicate our findings in people with diagnosed eating disorders using a longitudinal design.
- Published
- 2021
40. Pressure-induced ferroelectric transition in LiBC
- Author
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Hu Zhang, Lulu Zhao, Chendong Jin, Ruqian Lian, Peng-Lai Gong, RuiNing Wang, JiangLong Wang, and Xing-Qiang Shi
- Published
- 2022
41. A novel linear epitope at the C-terminal region of the classical swine fever virus E2 protein elicits neutralizing activity
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Shenli Zhang, Junqing Guo, Gaiping Zhang, Qianru Xu, Xiangxiang Niu, Yanwei Wang, Xueyang Li, Linke Liu, Qingmei Li, Shujun Chai, Yanan Wang, Ruining Wang, Li Wang, Yunchao Liu, Qiang Ma, Erqin Zhang, Fanshu Ma, and Min Jiang
- Subjects
Models, Molecular ,medicine.drug_class ,Peptide ,Monoclonal antibody ,Biochemistry ,Virus ,Epitope ,Epitopes ,Viral Envelope Proteins ,Antibody Specificity ,Peptide Library ,Structural Biology ,medicine ,Animals ,Amino Acid Sequence ,Molecular Biology ,Conserved Sequence ,chemistry.chemical_classification ,Mice, Inbred BALB C ,biology ,Linear epitope ,Pestivirus ,Antibodies, Monoclonal ,General Medicine ,biology.organism_classification ,Antibodies, Neutralizing ,Virology ,chemistry ,Classical Swine Fever Virus ,Classical swine fever ,biology.protein ,Female ,Antibody - Abstract
Classical swine fever virus (CSFV) is a member of the genus Pestivirus, which causes serious economic losses. The re-emergence of the disease in Japan in 2018 has increased awareness of CSFV. In this study, Balb/c mice were immunized with plant-derived E2 protein, and four monoclonal antibodies (mAbs) 4B11, 7B3, 11A5 and 6F3 were generated. Two of these mAbs, 4B11 and 7B3, effectively blocked CSFV infection of PK-15 cells. Both mAbs recognized a novel linear epitope, 256CLIGNTTVKVHASDER271. The neutralizing ability of anti-CSFV serum decreased 63%, when pre-incubated with the linear peptide at 200 μg/mL. Structural analysis showed that this linear epitope is present at the border of Domain C and Domain D on the surface of the E2 protein. Alignment of amino acid sequences showed that the epitope was conserved in different subgroups of CSFV but not in other members of the Pestivirus genus. Consistently with the analysis above, this epitope distinguished antibodies against CSFV from those against bovine viral diarrhea virus (BVDV). Our study provides an ideal candidate peptide for new vaccine design and differential diagnosis of CSFV. These findings will contribute to the control and eradication of classical swine fever.
- Published
- 2021
42. Designing a sustainable fluorescent targeting probe for superselective nucleus imaging
- Author
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Minghong Wu, Wei Ren, Weitao Li, Tao Xu, Robert Vajtai, Ming Li, Bin Wu, Yongle Li, Xing-Can Shen, Ruining Wang, Hailong Chen, Pulickel M. Ajayan, Jarin Joyner, Huazhang Guo, Mengying Le, and Liang Wang
- Subjects
Photoluminescence ,Biocompatibility ,Graphene ,Chemistry ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,law.invention ,Cell membrane ,Membrane ,medicine.anatomical_structure ,Quantum dot ,law ,Ultrafast laser spectroscopy ,medicine ,General Materials Science ,0210 nano-technology - Abstract
Specific targeting of cell organelles is indispensable for bioimaging and diagnostics but remains a challenge due to the limitation of highly selective fluorescent targeting probes. Herein, we successfully designed a molecular fusion route to controllably synthesize bright orange fluorescent graphene quantum dots (GQDs). The relative nitrogen doping level of the GQDs reached 18.88 at%. The GQDs exhibited long-wavelength excitation fluorescence and photoluminescence (PL) stability. Femtosecond transient absorption spectroscopy elucidated that the sp2 cluster and the surface state's synergistic effect contributed to the PL. Furthermore, the most alluring discovery was that the GQDs had good biocompatibility and an unprecedented targeting capability for superselective nucleus imaging without staining cell organelles. Molecular dynamics simulations revealed that once the GQDs were transported across the plasma membrane, the higher the N-doping ratio of the GQDs was, and the easier the GQDs penetrated the membrane. The reason may stem from the compatibility of –NH2 with lipid molecules, which is higher than that of –OH. Enriched nitrogen doping in GQDs is beneficial for crossing the cell membrane to the target nucleus. Our findings could provide scientific theory and a technical basis to design nucleus-targeting probes in the future.
- Published
- 2021
43. Extract Chinese Unknown Words from a Large-scale Corpus Using Morphological and Distributional Evidences.
- Author
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Kaixu Zhang, Ruining Wang, Ping Xue 0002, and Maosong Sun 0001
- Published
- 2011
44. Study on the line and station of the high speed railway in China: take the Beijing-Shanghai high-speed railway as an example
- Author
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Xuesi Duan, Ruining Wang, and Shuying Wang
- Published
- 2022
45. THE INFLUENCE OF CARBON NANOTUBE ADDITION ON THE SHEAR-THICKENING PERFORMANCE OF SUSPENSIONS.
- Author
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Ruining WANG, Yingying ZHOU, Qiushi WANG, Runjun SUN, Xiaoya JIA, and Mingyue TIAN
- Subjects
- *
CARBON nanotubes , *MULTIWALLED carbon nanotubes , *YARN , *RHEOLOGY , *SILICA fume , *SHEAR strength - Abstract
The shear thickening fluid as a protective material has received increasing attention, and its impact resistance and its rheological properties are controllable by integrating various kinds of additives to a single phase shear thickening fluid. In this paper, the rheological properties of shear thickening fluids with 26 wt.% fume silica, PEG200 and different mass fraction of multi-walled carbon nanotubes are investigated, and the effect of temperature from -5 °C to 55 °C on steady state rheological properties of 1.0 wt.% multi-walled carbon nanotubes reinforced shear thickening fluids is studied. Finally a single yarn pull-out test is conducted to examine the influence of multi-shear thickening fluid on the shear strength and inter-yarn friction of fabrics. The results show that the addition of multi-walled carbon nanotubes can improve significantly the viscosity and shear thickening efficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
46. Exploring alexithymia profiles and their associations with childhood adversity and COVID-19 burnout among Chinese college students: A person-centered approach
- Author
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Ruining Wang, Baojuan Ye, Peiyi Wang, Qiang Yang, and Siming Luo
- Subjects
Developmental and Educational Psychology - Published
- 2023
47. Genome-wide differential expression of long noncoding RNAs and mRNAs in ovarian follicles of two different chicken breeds
- Author
-
Ziya Zhao, Lulu Hu, Xianglong Li, Yuanfang Gong, Zhengzhu Liu, Chuansheng Zhang, Ruining Wang, Jingshi Li, Liying Geng, Li Chang, Yaqi Wang, and Yongdong Peng
- Subjects
0106 biological sciences ,China ,endocrine system ,Biology ,01 natural sciences ,Genome ,03 medical and health sciences ,Ovarian Follicle ,Follicular phase ,Genetics ,medicine ,Animals ,Gene Regulatory Networks ,RNA, Messenger ,Differential expression ,Gene ,030304 developmental biology ,Regulation of gene expression ,0303 health sciences ,Gene Expression Profiling ,Cell cycle ,Oocyte ,Breed ,Cell biology ,medicine.anatomical_structure ,Female ,RNA, Long Noncoding ,Chickens ,010606 plant biology & botany - Abstract
Bashang long-tail chickens are an indigenous breed with dual purpose in China (meat and eggs) but have low egg laying performance. To improve the low egg laying performance, a genome-wide analysis of mRNAs and long noncoding RNAs (lncRNAs) from Bashang long-tail chickens and Hy-Line brown layers was performed. A total of 16,354 mRNAs and 8691 lncRNAs were obtained from ovarian follicles. Between the breeds, 160 mRNAs and 550 lncRNAs were found to be significantly differentially expressed. Integrated network analysis suggested some differentially expressed genes were involved in ovarian follicular development through oocyte meiosis, progesterone-mediated oocyte maturation, and cell cycle. The impact of lncRNAs on cis and trans target genes, indicating some lncRNAs may play important roles in ovarian follicular development. The current results provided a catalog of chicken ovarian follicular lncRNAs and genes for further study to understand their roles in regulation of egg laying performance.
- Published
- 2019
48. Coronavirus Stress and Overeating: The Mediating Role of Anxiety and the Moderating Role of COVID-19 Burnout
- Author
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Ruining Wang, Baojuan Ye, and Qiang Yang
- Abstract
Background: This study examined the mediating effect of anxiety and the moderating effect of COVID-19 burnout on the relationship between coronavirus stress and overeating among Chinese college students during the COVID-19 period. Methods: 2926 Chinese students (Mage = 19.90, SD = 1.47, range = 18 - 25, 54.3% female) completed self-reported questionnaires regarding coronavirus stress, anxiety, overeating, and COVID-19 burnout. The data was analyzed using Pearson’s r correlations and moderated mediation analysis. Results: The results revealed that anxiety mediated the link between coronavirus stress and college students’ overeating. College students’ COVID-19 burnout moderated the associations between anxiety and college students’ overeating. The association between coronavirus stress and anxiety was stronger for those with higher COVID-19 burnout. Discussion and conclusion: Findings of this study demonstrated that coronavirus stress was positively related to anxiety, which in turn, was related to overeating in college students. COVID-19 burnout enhanced this effect at indirect mediation paths, suggesting that college students with greater COVID-19 burnout may be more likely to have overeating behavior. This study identified the critical factors associated with overeating; it supplies empirical support for existing theories and provides practical implications for interventions aiming to decrease Chinese college students’ overeating during COVID-19 period.
- Published
- 2021
49. Sequence Analysis of
- Author
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Mengmeng, Zhao, Huawei, Li, Hang, Zhang, Huiyang, Sha, Liangzong, Huang, and Ruining, Wang
- Abstract
Monkey diseases are becoming increasingly severe, and some may be transmitted to humans through direct and indirect contact. Innate immunity is the first line of defense against foreign microorganisms. It is of great significance to explore the immune characteristics of monkey and human diseases. TRIM4, an important immune molecule in
- Published
- 2021
50. TRIM4-mediated ubiquitination of NSP2 restricts porcine reproductive and respiratory syndrome virus proliferation
- Author
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Mengmeng Zhao, Huiyang Sha, Hang Zhang, and Ruining Wang
- Subjects
Proteomics ,General Veterinary ,Swine ,Ubiquitination ,Animals ,Porcine respiratory and reproductive syndrome virus ,General Medicine ,Virus Replication ,Antiviral Agents ,Cell Proliferation - Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and virulent infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV), which has substantial economic losses in the pig industry worldwide, and PRRSV attenuated vaccines and inactivated vaccines do have limitations in immune protection. The discovery of new antiviral targets has become a new research field. The proteomic studies have shown that the PRRSV NSP2 protein interacts with tripartite motif protein 4 (TRIM4), but it was still unknown whether TRIM4 regulates PRRSV infections. In this study, the TRIM4 gene from Marc-145 cells was cloned, and it was proved that TRIM4 overexpression inhibits PRRSV replication, whereas TRIM4 small-interfering-RNA knockdown resulted in increased virus titers. Mechanism investigation indicated that TRIM4 inhibits PRRSV replication through ubiquitination and degradation of the NSP2 protein. Protease inhibitor MG132 (carbobenzoxy-Leu-Leu-leucinal) attenuated the TRIM4-driven degradation of NSP2. Taken together, TRIM4 impairs PRRSV proliferation via ubiquitination and degradation of NSP2.
- Published
- 2021
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