Search

Genetic mutations that cause defective mismatch repair, may result in microsatellite instability, which is common in colorectal carcinoma and gastric cancers as well as Lynch syndrome. Mismatch repair deficiency/microsatellite instability-high(dMMR/MSI-H) predicts the tumor response to immune checkpoint inhibitors. However, few studies have evaluated the effects of immune checkpoint inhibitors on dMMR/MSI-H non-small cell lung cancer (NSCLC). We report here a squamous lung cancer patient with dMMR/MSI-H and high tumor mutational burden(TMB-H) who obtained the long-term benefit from immunotherapy. We consider that NSCLC patient with dMMR/MSI-H can benefit from immune checkpoint inhibitors.

A 55-year-old Chinese man with a right lung mass and lymph node metastasis (T4N3M0 IIIB) was diagnosed with lung adenocarcinoma after a CT-guided biopsy. With the wide application of next-generation sequencing (NGS) in tumour detection, we found a rare CCDC85A-ALK fusion. The patient received alectinib, which had marked efficacy. This is the first report of a lung adenocarcinoma patient harbouring a new uncommon anaplastic lymphocyte kinase fusion that showed a remarkable response to alectinib. NGS aids in selecting treatment in non-small cell lung cancer patients.

The efficacy of first-and second-generation epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in NSCLC patients with the EGFR L861Q mutation has been studied previously. However, there is little evidence on the efficacy of osimertinib in NSCLC patients with uncommon mutations. Here, we report the case of a 68-year-old man with advanced NSCLC with concurrent EGFR L861Q mutation as well as TP53 and RB1 mutations. The patient was treated with osimertinib as first-line therapy and achieved a remarkable progression-free survival of 15 months. His symptoms were significantly alleviated and the dose was well tolerated. The findings of the present study indicate that osimertinib might be a good treatment option for NSCLC patients with the L861Q mutation.

Despite the rapid development of two-dimensional covalent organic frameworks (2D COFs) in recent years, it remains a great challenge to synthesize highly crystalline COF materials. Here, a CNC-assisted approach was adopted to synthesize high crystallinity COF materials. A series of 2D COF films were synthesized at the air���water interface by using cellulose nanocrystals (CNCs) as the template. The occurrence of Schiff reactions based on the imine bond was demonstrated by Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) exhibited the appearances of 2D COF films were flower-like. When CNCs were added to a certain extent, the size of a single petal in the flowers gradually increased with the amount of CNCs. The film with large petals was characterized by Ultraviolet���Visible diffuse reflectance spectroscopy (UV���Vis DRS), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and selected area electron diffraction (SAED). In UV���Vis DRS curves, the S-band of COF-366 film was red-shifted by 24 nm compared with that of 5,10,15,20-tetrakis(4-aminophenyl)-21H,23H-porphyrin (TAPP), confirming the existence of extended conjugation in COF-366 film. XPS was used to identify the surface composition of the sample. The N1s signal of the film indicated that each TAPP formed four imine bonds with 2,5-dihydroxyterephthalaldehyde (DHTA) in COF-366 film. TEM images showed that CNCs had an influence on the crystal size. It was observed from SAED that the crystallinity of the film with CNCs was higher than the film without CNCs. This work provided a new template for improving the crystallinity of 2D COF films., publishedVersion

In this work, self-healing chiral photonic films (SCPFs) fabricated by the co-assembly of cellulose nanocrystals (CNCs) with a boronic ester crosslinked poly(vinyl alcohol)–polyacrylamide hydrogel is described. The reversible covalent bonds of boronic esters enable the self-healing of SCPFs, the convenient formation of patterns/stacked structures, and the incorporation of SCPFs onto rough and porous surfaces, addressing a universal method for constructing photonic structures on various substrates. The double layer structures of SCPFs with bulk hydrogels enable multidimensional sensing via dynamic reflectance spectroscopy. The unique dynamic reflectance spectral patterns, as well as regular changes in reflectance maxima and delay time, demonstrate a sensitive photonic sensor. As a result of the self-healing properties, the robust anti-counterfeiting layers formed by the SCPFs on paper-printed QR codes realize polarization-dependent recognitions by a smartphone. The approach allows the incorporation of chiral photonic crystals onto various substrates with high optical quality, providing flexibility to design chiral photonic structures for sensing and security applications.

Colorectal cancer is with high incidence worlwide.. Because of the heterogeneity of the tumor, combination therapy is probably of great significance to improve the prognosis of colorectal cancer patients. Herein, the pH-responsive supramolecular hydrogels mPEG-luteolin-BTZ@ICG based on bortezomib (BTZ) and indocyanine green (ICG) were prepared, and the colorectal cancer was treated with mPEG-luteolin-BTZ@ICG through the combination of photothermal/photodynamic and chemotherapy. BTZ performed drug therapy, meanwhile ICG wrapped in supramolecular hydrogels possessed higher light stability than free ICG to perform photothermal/photodynamic therapy. In vitro and in vivo assays showed excellent inhibition of tumor cells due to the combined effect of BTZ and ICG. The mPEG-luteolin-BTZ@ICG combined with laser therapy possessed exceptional biological safety and provided new candidates for advanced colon cancer therapy and important references for other tumor therapies.

The aim of this study was to prepare APAP crystals by cooling, anti-solvent, and solvent evaporation crystallization to enhance its dissolution rate and to make comparisons of the three methods. Agitating speeds and types were regarded as factors affecting crystallization procedure. Samples were made with different ratios of PEG4000. They were characterized by X-ray diffraction and scanning electron microscopy. Dissolution tests were conducted to assess their dissolution property. The proportions of carriers existing in crystals by cooling and anti-solvent crystallization ranged from 1.3 to 5.1%. Mean dissolution time (MDT) of samples by the two methods was about 3 min, which was 17.2 min for untreated APAP. Addition of too much PEG4000 in solvent evaporation crystallization could decrease dissolution rate of APAP. Samples agitated by a rotor with speed of 100, 500, and 1000 rpm dissolved faster than those by a high shear mixer with speed of 3400 and 5000 rpm or by a glass rod. Agitating speed and type could affect particle size and drug dissolution. Dissolution enhancement of APAP might be attributed to decrease of fine particles and increase of particle wettability.

The previous investigation has proved that their existed pharmacokinetic difference between the different crystal forms of the polymorphic drugs after oral administration. However, no systemic investigations have been made on the change of this pharmacokinetic difference, resulted either from the physiological or from the pathological factors. In this paper, we used polymorphic nimodipine (Nim) as a model drug and investigated the effect of age difference (2- and 9-month old) on the pharmacokinetics after oral delivery in rats. As the results shown, for L-form of Nim (L-Nim), the AUC0–24 h in 2-month-old rats was 343.68±47.15 ng·h/mL, which is 23.36% higher than that in 9-month-old rats. For H-form of Nim (H-Nim), the AUC0–24 h in 2-month-old rats was 140.91±19.47 ng·h/mL, which is 54.64% higher than that in 9-month-old rats. The AUC0–24 h ratio between H-Nim and L-Nim was 2.44 in 2-month-old rats and 3.06 in 9-month-old rats. Since age difference could result in unparallelled change of the absorption and bioavailability of the polymorphic drugs, the results in this experiment are of value for further investigation of crystal form selection in clinical trials and rational clinical application of the polymorphic drugs., Graphical abstract In this paper, polymorphic nimodipine (Nim) was used as a model drug to investigate the effect of age difference (2- and 9-month old) on the pharmacokinetics after oral delivery in rats. Since age difference could result in unparalleled change of the absorption and bioavailability of the polymorphic drugs, the results in this experiment are of value for further investigation of crystal form selection in clinical trials and rational clinical application of the polymorphic drugs. fx1

To investigate the effects of Xinfeng Capsule (XFC) on pulmonary function and related mechanism in adjuvant-induced arthritis (AA) rats.The rats were randomly divided into five groups: normal control (NC), model control (MC) groups, methotrexate (MTX), tripterygium glycosides tablet (TPT) and Xinfeng capsule (XFC) treatment groups. The adjuvant-induced arthritis model was established by intracutaneous injection of 0.1 mL Freund ' s complete adjuvant in the right paw of rats; the drugs were given 19 d after model establishment. The toe swelling degree (E), arthritis index (AI), pulmonary function, peripheral blood Treg levels, pathological changes of lung tissue and expression of Foxp3, TGF-β1, Smad3, Smad7 proteins in lung tissue were measured 30 d after drug administration.Compared to NC group, the levels of E, AI, alveolitis score, TGF-β1 and Smad3 were significantly increased (P0.05 or P0.01); maximum expiratory flow 25% of vital capacity (FEF25),50% maximal expiratory vital capacity flow (FEF50), maximum expiratory flow at 75% of vital capacity (FEF75), maximum mid-expiratory flow (MMF), force peak expiratory flow (PEF), CD4+ CD25+ Treg, Foxp3 and Smad7 were significantly decreased in MC group (P0.05 or P0.01). Compared to MC group,the expression of E, AI, TGF-β1 and Smad3 were reduced, while FEF50, FEF75, MMF, PEF, Treg, Foxp3 and Smad7 were elevated in XFC group (P0.05 or P0.01). Compared to XFC group, the level of body mass,FEF25,FEF50, FEF75, MMF and Treg were lower in MTX and TPT groups (P0.05 or P0.01).There are inflamed joints and reduced pulmonary function in rats of adjuvant-induced arthritis. XFC can inhibit paw edema degrees, reduce arthritis response, and improve pulmonary function, which is associated with up-regulating expression of Treg and Foxp3, down-regulating the expression of TGF-β1 and adjusting TGF-β1/Smads signal pathway.

Objective To observe the impact of xinfeng xapsule (XFC) on pulmonary function in a rat model of adjuvant arthritis (AA) and to investigate the mechanism of action. Methods Forty rats were randomly divided into four groups of ten: normal control (NC); model control (MC); tripterygium glycosides tablet (TPT); and xinfeng capsule (XFC). Except for the NC group, AA was induced in all rats by intracutaneous injection of 0.1 mL Freund's complete adjuvant in the right paw on the 19th day. NC and MC groups were given (0.9%) physiological saline. The TPT and XFC groups were given TPT (10 mg/kg) and XFC (1.2 g/kg), respectively. Thirty days after administration, changes in paw edema (E), the arthritis index (AI), pulmonary function, levels of regulatory T-cells (Treg), ultrastructure of lung tissue, and expression of Notch receptors and ligands in lung tissue were observed. Results In the MC group, E and the AI were increased and pulmonary function significantly decreased; the structure of alveolar type-II cells was damaged; ratios of Treg in peripheral blood were reduced; and expression of Notch receptors such as Notch3 and Notch4 and ligands such as Delta1 in lung tissue were significantly increased whereas expression of Notch1, Jagged1 and Jagged2 were significantly decreased. After intervention with XFC, E and the AI were decreased; pulmonary function was enhanced; the structure of alveolar type-II cells was improved; and expression of Treg, Notch1, Jagged1, Jagged2 was elevated, whereas that of Notch3, Notch4 and Delta1 was reduced. Conclusion XFC can not only inhibit E and the AI and improve joint symptoms, it can also improve pulmonary function and reduce inflammation in lung tissue. These actions could be carried out through increases in the expression of Treg, Notch receptors (Notch1) and ligands (Jagged1, Jagged2), and reductions in the expression of Notch3, Notch4 and Delta1. These phenomena would reduce the deposition of immune complexes and the inflammatory response in lung tissue, thereby improving joint symptoms and pulmonary function.

Since the historic 11th plenary session of the Chinese Communist Party, China has taken bold measures to introduce and carry out a comprehensive range of reform and opening up to the outside. In as little as 20 years, great changes have occurred across the country. Guandong, a pioneer province in China implementing such reform policy, has distinguished itself with an astonishn and greatly enhanced economic strength. This study explores the adminitrative reform in Guangdon province and its characteristics.