247 results on '"Rui-Hong Wang"'
Search Results
2. The clinical value of neutrophil-to-lymphocyte ratio (NLR), systemic immune-inflammation index (SII), platelet-to-lymphocyte ratio (PLR) and systemic inflammation response index (SIRI) for predicting the occurrence and severity of pneumonia in patients with intracerebral hemorrhage
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Rui-Hong Wang, Wan-Xin Wen, Ze-Ping Jiang, Zhen-Ping Du, Zhao-Hui Ma, Ai-Li Lu, Hui-Ping Li, Fang Yuan, Shi-Biao Wu, Jian-Wen Guo, Ye-Feng Cai, Yan Huang, Li-Xin Wang, and Hong-Ji Lu
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stroke-associated pneumonia ,NLR ,SII ,PLR ,SIRI ,prediction value ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundInflammatory mechanisms play important roles in intracerebral hemorrhage (ICH) and have been linked to the development of stroke-associated pneumonia (SAP). The neutrophil-to-lymphocyte ratio (NLR), systemic immune-inflammation index (SII), platelet-to-lymphocyte ratio (PLR) and systemic inflammation response index (SIRI) are inflammatory indexes that influence systemic inflammatory responses after stroke. In this study, we aimed to compare the predictive value of the NLR, SII, SIRI and PLR for SAP in patients with ICH to determine their application potential in the early identification of the severity of pneumonia.MethodsPatients with ICH in four hospitals were prospectively enrolled. SAP was defined according to the modified Centers for Disease Control and Prevention criteria. Data on the NLR, SII, SIRI and PLR were collected at admission, and the correlation between these factors and the clinical pulmonary infection score (CPIS) was assessed through Spearman’s analysis.ResultsA total of 320 patients were enrolled in this study, among whom 126 (39.4%) developed SAP. The results of the receiver operating characteristic (ROC) analysis revealed that the NLR had the best predictive value for SAP (AUC: 0.748, 95% CI: 0.695-0.801), and this outcome remained significant after adjusting for other confounders in multivariable analysis (RR=1.090, 95% CI: 1.029-1.155). Among the four indexes, Spearman’s analysis showed that the NLR was the most highly correlated with the CPIS (r=0.537, 95% CI: 0.395-0.654). The NLR could effectively predict ICU admission (AUC: 0.732, 95% CI: 0.671-0.786), and this finding remained significant in the multivariable analysis (RR=1.049, 95% CI: 1.009-1.089, P=0.036). Nomograms were created to predict the probability of SAP occurrence and ICU admission. Furthermore, the NLR could predict a good outcome at discharge (AUC: 0.761, 95% CI: 0.707-0.8147).ConclusionsAmong the four indexes, the NLR was the best predictor for SAP occurrence and a poor outcome at discharge in ICH patients. It can therefore be used for the early identification of severe SAP and to predict ICU admission.
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- 2023
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3. Plio-Pleistocene climatic change drives allopatric speciation and population divergence within the Scrophularia incisa complex (Scrophulariaceae) of desert and steppe subshrubs in Northwest China
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Rui-Hong Wang, Zhao-Ping Yang, Zhi-Cheng Zhang, Hans Peter Comes, Zhe-Chen Qi, Pan Li, and Cheng-Xin Fu
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allopatric speciation ,aridification ,divergence ,phylogeography ,Scrophularia incisa complex ,Plant culture ,SB1-1110 - Abstract
Numerous temperate plants and animals on the Qinghai-Tibet Plateau (QTP) are hypothesized to have differentiated due to vicariant allopatric speciation associated with the geologic uplifts. However, this hypothesis has rarely been tested through a phylogeographic study of relative species in a broader geographic context, including the QTP, Tianshan Mountains, Mongolian Plateau, and surrounding regions. To understand the speciation and diversification process of plants across this wide area, phylogeographic analysis were examined from Scrophularia incisa and two other closely relative species comprising S. kiriloviana and S. dentata. Thirty-two populations of the three close relatives were genotyped using chloroplast DNA fragments and nuclear microsatellite loci to assess population structure and diversity, supplemented by phylogenetic dating, ancestral area reconstructions and species distribution modelings, as well as niche identity tests. Our chloroplast DNA (cpDNA) phylogeny showed that this monophyletic group of desert and steppe semi-shrub is derived from a Middle Pliocene ancestor of the Central Asia. Lineages in Central Asia vs. China diverged through climate/tectonic-induced vicariance during Middle Pliocene. Genetic and ENM data in conjunction with niche differentiation analyses support that the divergence of S. incisa, S. dentata and S. kiriloviana in China lineage proceeded through allopatric speciation, might triggered by early Pleistocene climate change of increase of aridification and enlargement of deserts, while subsequent climate-induced cycles of range contractions/expansions enhanced the geographical isolation and habit fragmentation of these taxa. These findings highlight the importance of the Plio-Pleistocene climate change in shaping genetic diversity and driving speciation in temperate steppes and deserts of Northwestern China.
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- 2022
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4. Editorial: Uncovering Drug Resistance During Cancer Therapy
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Haitao Wang, Rui-Hong Wang, Jian-Guo Zhou, Weilong Hou, and Ada Hang-Heng Wong
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cancer ,oncology ,cancer therapy ,drug resistance ,next generation sequencing ,biomarkers ,Genetics ,QH426-470 - Published
- 2022
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5. The complete chloroplast genome sequence of Spiraea×vanhouttei (Briot) Zabel (Rosaceae)
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Min-min Chen, Rui-hong Wang, Hong-kun Sha, Ming-zhi Liu, Jian-quan Tong, and Qiu-Ling He
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spiraea×vanhouttei ,spiraea ,chloroplast genome ,phylogeny ,Genetics ,QH426-470 - Abstract
Spiraea×vanhouttei (Rosaceae) is a frequently planted Spiraea species that is distributed in Shandong Province, Jiangsu Province, and Guangdong Province, China. The first complete chloroplast genome of Spiraea×vanhouttei was determined and described in this study. The genome is 155,957 bp in length and contained 129 encoded genes in total, including 84 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. The phylogenomic analysis showed that Spiraea×vanhouttei was closely related to Spiraea blumei according to the current sampling extent.
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- 2022
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6. The complete chloroplast genome of medicinal herb Reynoutria japonica Houtt. (Polygonaceae)
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Sanjana Vijaya Candassamy, Jun-Jie Wu, Xuan Zhou, Rui-Hong Wang, Zhe-Chen Qi, and Xiao-Ling Yan
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reynoutria japonica ,chloroplast genome ,phylogenetics ,medicinal herb ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of medicinal Reynoutria japonica Houtt. was sequenced and assembled in this study. The genome is 163,410 bp in length and contained 129 encoded genes in total, including 86 protein-coding genes, 8 ribosomal RNA genes, and 36 transfer RNA genes. The phylogenetic tree based on 14 chloroplast genomes in Polygonaceae support that R. japonica is related to Muehlenbeckia.
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- 2020
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7. The complete chloroplast genome sequence of Spiraea japonica var. acuminata Franch. (Rosaceae)
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Qi Wang, Min-min Chen, Xia-fang Hu, Rui-hong Wang, and Qiu-Ling He
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spirea japonica ,spirea japonica var. acuminata franch. ,chloroplast genome ,phylogeny ,Genetics ,QH426-470 - Abstract
Spirea japonica var. acuminata Franch. (Rosaceae) is a Chinese herbal medicine distributed in southwest and east China. The first complete chloroplast genome of Spirea japonica var. acuminata Franch. was assembled and reported in this study. The genome is 153,822 bp in length and contained 125 encoded genes in total, including 80 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. The phylogenomic analysis showed that Spirea japonica var. acuminata Franch. was closely related to Spirea blumei, Spirea trilobata, Spirea mongolica and Spirea insularis according to the current sampling extent.
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- 2022
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8. The complete chloroplast genome of balsam aster (Aster ageratoides Turcz. var. scaberulus (Miq.) Ling., Asteraceae)
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Jie-Ying Feng, Yun-Zhe Wu, Rui-rui Wang, Xian-Fei Xiao, Rui-Hong Wang, Zhe-Chen Qi, and Xiao-Ling Yan
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aster ageratoides ,asteraceae ,chloroplast genome ,phylogenomic analysis ,Genetics ,QH426-470 - Abstract
The first complete chloroplast genome of Aster ageratoides Turcz. var. scaberulus (Miq.) Ling. is reported in this study. The total chloroplast genome size of A. ageratoides var. scaberulus was 153,071 bp and comprised of a large single-copy region (LSC with 84,896 bp), a small single-copy region (SSC with 18,269 bp), and two inverted repeat regions (IR with 24,953 bp). A total of 122 genes were included in the genome, including 83 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. Eleven protein-coding genes had intron (ycf3, clpP and rps12 gene contained two introns. Further phylogenomic analysis of Asteraceae, including 13 taxa, was conducted for the placement of A. ageratoides var. scaberulus.
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- 2021
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9. The complete chloroplast genome sequence of wild Japanese pepper Tubocapsicum anomalum Makino (Solanaceae)
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Rui-Hong Wang, Min-Min Chen, Yun-Zhe Wu, Rui-rui Wang, Xian-Fei Xiao, Zhe-Chen Qi, and Xiao-Ling Yan
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tubocapsicum anomalum ,chloroplast genome ,phylogeny ,Genetics ,QH426-470 - Abstract
As an important medicinal herb, no complete organelle molecular data has been reported for Tubocapsicum anomalum. In this study, the first complete chloroplast genome of Tubocapsicum anomalum Makino was sequenced and assembled. The genome is 155,802 bp in length and contained 124 encoded genes in total, including 75 protein-coding genes, 10 ribosomal RNA genes, and 39 transfer RNA genes. The phylogenomic analysis showed that Tubocapsicum anomalum was closely related to Withania somnifera according the current sampling extent.
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- 2021
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10. Cyclin B1 stability is increased by interaction with BRCA1, and its overexpression suppresses the progression of BRCA1-associated mammary tumors
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Eun Kyung Choi, Jeong-A Lim, Jong Kwang Kim, Moon Sun Jang, Sun Eui Kim, Hye Jung Baek, Eun Jung Park, Tae Hyun Kim, Chu-Xia Deng, Rui-Hong Wang, and Sang Soo Kim
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Medicine ,Biochemistry ,QD415-436 - Abstract
Breast cancer: A protein link for treatment? The role of disrupted activity of the protein BRCA1 in the progression of breast cancer has been clarified, suggesting that targeting another protein with which it interacts could offer a new route to treatment. Mutations of BRCA1 are known to predispose women to both breast and ovarian cancers. Researchers led by Sang Soo Kim (National Cancer Center, South Korea) and Rui-Hong Wang (University of Macau, China) studied the interaction with a protein called cyclin B1 that controls cell growth and division. They found that, in mitosis, BRCA1 interacts with and stabilizes cyclin B1, explaining why the loss of BRCA1 can disrupt the G2/M cell cycle control and accumulate the genetic instability. Treatment of Brca1-mutant mammary tumors with vinblastine, which alters cyclin B1 level, significantly reduced tumor progression with reduction of survival and induction of apoptosis.
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- 2018
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11. Clinical application of locational partition of the nasal endsin lacerated lacrimal canaliculi
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Na Liang, Ning Chen, Rui-Hong Wang, Yu-Hong Li, Ji-Tao Zhang, undefined, Hai-Peng Ma, and Juan Liu
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canalicular laceration ,nasal ends ,temporal ends ,lacrimal caruncle area ,medial canthal ligament area ,Ophthalmology ,RE1-994 - Abstract
AIM: To investigate the positional relationship between the nasal ends of the lacerated lacrimal canaliculi, lacrimal caruncle, and medial canthal ligament, as well as its application in the lacrimal canalicular anastomosis.METHODS: Sixty-five patients with traumatic lower canalicular laceration were selected. The distance from the lacrimal punctum to the temporal end was measured. The nasal ends were found under direct vision, and the positional relationship between the nasal ends, lacrimal caruncle and medial canthal ligament were quantitatively analyzed.RESULTS: Totally 65 patients with canaliculus laceration, 39 patients had a distance of less than 6mm from the lacrimal punctum to the temporal end, where the nasal ends of 35 patients were located in the lacrimal caruncle area. There were 26 cases in which the distance from the lacrimal punctum to the temporal end was greater than 6mm, where the nasal ends of 24 cases were located in the medial canthal ligament area. The vertical distance between the nasal ends in the lacrimal caruncle area and the lacrimal mucosa was 2.1±0.7mm, and the vertical distance between the nasal ends in the medial canthal ligament area and the skin edge was 3.5±1.2mm.CONCLUSION: The positions of nasal ends can be divided into the lacrimal caruncle area and the medial canthal ligament area, providing a basis for finding the nasal ends of the lacerated lacrimal canaliculi in the clinical practice.
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- 2019
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12. Clinical study of two kinds of lacrimal duct catheterization in the treatment of lacrimal canalicular rupture in children
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Rui-Hong Wang, Na Liang, and Hong-Ying Du
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lacrimal canaliculi rupture ,RS lacrimal tube ,double silicone tube ,lacramal intubation ,children ,Ophthalmology ,RE1-994 - Abstract
AIM: To explore the usefulness of RS-type lacrimal drainage tube versus double silicone tube for children's lacrimal canaliculi rupture.METHODS: A retrospective analysis from August 2016 and July 2018 were taken. Totally 37 children(37 eyes)of lacrimal canaliculi rupture were enrolled in the study and divided into two groups according to admission time sequence: treatment group(20 eyes)and control group(17 eyes). The treatment group was treated with RS-type lacrimal drainage tube. The control group underwent the implantation of double silicone tube and their efficacy was analyzed.RESULTS: It was significant difference that the epistaxis rate between the treatment group and the control group, which was 0% and 100%. The effective rate was 95% and 88%, respectively, for the treatment group and the control group, the difference between the two groups has no significant(P=0.584). And the postoperative complication rate was 5% in treatment group, 12% in control group respectively, there was no significant statistical difference between the two groups(P=0.584).CONCLUSION: The implantation of the RS-type lacrimal drainage tube can effectively prevent epistaxis, is safe and simple, easy to operate and extubate easily with good clinical effect in the treatment of lacrimal canaliculus fracture in children. So it can be widely carried out in basic hospitals.
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- 2019
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13. Clinical outcomes of RS lacrimal tube for lacrimal canaliculi rupture without nasal endoscope
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Rui-Hong Wang, Ning Chen, Shu-Jun Zhang, Rong-Hua Li, Na Liang, Hai-Peng Ma, Ji-Tao Zhang, Yu-Hong Li, and Li-Kun Guo
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lacrimal canaliculi rupture ,RS lacrimal tube ,annular silicone tube ,silicone tube ,Ophthalmology ,RE1-994 - Abstract
AIM:To explore the clinical outcomes of RS lacrimal tube for lacrimal canaliculi rupture without nasal endoscope.METHODS:A retrospective analysis from September 2017 to March 2018 were taken. According to admission time sequence. Totally 52 patients with lacrimal canaliculi rupture was divided into two groups. One group was treated with RS lacrimal tube, the other was treated with annular silicone tube. The treatment results were comparatively analyzed. RESULTS:There were 15 eyes of nasal mucosa hemorrhage in annular silicone tube group. There was no eye with this in RS tube group, the difference between the two groups was significant(PP=0.877). There were 2 eyes in RS tube group occurred fall off in 2wk to 2mo after operations; 1 eye of lacrimal puncture were removed from the lacrimal puncture without 2wk, and was anastomosed again. The other eye was removed from the lacrimal puncture within 1-2mo after the operation, the lacrimal canal flushing was improved during follow-up. There were 3 eyes in annular silicone group occurred fall off without 2wk, and were not anastomosed again. The lacrimal duct flushing was improved during follow-up. No statistical significance was found in the two groups(P=0.361). CONCLUSION:Lacrimal canaliculi rupture should be timely given surgical treatment. RS lacrimal tube can not only achieve good operation effect and reduce the probability of complications, but also has the advantages of simple surgical methods, good histocompatibility and no influence on the appearance of patients.
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- 2019
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14. The complete chloroplast genome sequences of Hippuris vulgaris (Plantaginaceae)
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Zheng-Ying You, Lu-Jie Ren, Yu-Dong Li, Jia-Nan Ying, Ying Zhang, Yin-Jiao Yu, Xin-Wei Xu, Rui-Hong Wang, Zhe-Chen Qi, and Qi-Xiang Lu
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chloroplast genome ,hippuris vulgaris ,plantaginaceae ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
Two complete chloroplast genomes of Hippuris vulgaris (H. vulgaris_A and H. vulgaris_B), representing two distinct clades in China, were sequenced and assembled in this study. The circular genomes were 152,763 and 152,713 bp in length and exhibit a typical quadripartite structure of the large single-copy (LSC, 82,983/82,949 bp) and small single-copy (SSC, 18,294/18,278 bp) regions, separated by a pair of inverted repeats (IRs, both 25,743 bp). Both two cp genomes identically contain 133 genes, including 88 protein-coding genes, 37 tRNA, and eight rRNA genes. The phylogenetic analysis within Plantaginaceae demonstrated Hippuris an independent clade included in the expanded Plantaginaceae.
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- 2021
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15. The complete chloroplast genome of endangered Zhangjiajie sage Salvia daiguii Y. K. Wei & Y. B. Huang (Lamiaceae)
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Xuan Zhou, Yan-Bo Huang, Zhi-Cheng Zhang, Xin-Yan Xu, Rui-Hong Wang, Ling Xu, Zhe-Chen Qi, and Yu-Kun Wei
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salvia daiguii ,chloroplast genome ,endangered species ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of Zhangjiajie sage, Salvia daiguii, was assembled in this study. The genome is 151,434 bp in length and contained 134 encoded genes in total, including 88 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. The result of phylogenetic analysis based on 17 chloroplast genomes revealed that S. daiguii is clustered with Salvia miltiorrhiza in Lamiaceae.
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- 2020
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16. The complete chloroplast genome of Russian sage Salvia yangii B. T. Drew (Lamiaceae)
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Meng-Ting Cao, Jun-Jie Wu, Rui-Hong Wang, Ling Xu, Zhe-Chen Qi, and Yu-Kun Wei
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salvia yangii ,perovskia atriplicifolia ,chloroplast genome ,phylogenetics ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of Russian sage Salvia yangii B. T. Drew was assembled in this study. The genome is 151,473 bp in length and contained 129 encoded genes in total, including 84 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. The result of phylogenetic analysis based on 15 chloroplast genomes revealed that S. yangii is closely related to common sage (Salvia officinalis) in Lamiaceae.
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- 2020
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17. The complete chloroplast genome sequence of Palmer Amaranth (Amaranthus palmeri S. Watson)
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Xin-Yan Xu, Jun-Jie Wu, Jing Yan, Xuan Zhou, Rui-Hong Wang, Zhe-Chen Qi, and Xiao-Ling Yan
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amaranthus palmeri ,chloroplast genome ,invasive plant ,phylogenetics ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of Palmer Amaranth Amaranthus palmeri was sequenced and assembled in this study. The genome is 150,721 bp in length and contained 129 encoded genes in total, including 84 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. The phylogenetic tree based on 12 chloroplast genomes in Amaranthaceae support that A. palmeri is sister to A. hypochondriacus and A. caudatus.
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- 2020
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18. The complete chloroplast genome of Mexican marigold (Tagetes erecta L., Asteraceae)
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Zhi-Cheng Zhang, Shu-Hang Hu, Yong-Qing Peng, Hai-Shan Yan, Fan Xiao, Jing Gao, Jun-Jie Wu, Xuan Zhou, Xin-Yan Xu, Ling Xu, Rui-Hong Wang, and Zhe-Chen Qi
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tagetes erecta ,chloroplast genome ,ornamental plant ,mexican marigold ,Genetics ,QH426-470 - Abstract
Tagetes erecta is an important ornamental and medicinal plant indigenous to Mexico and Guatemala. The complete chloroplast genome of T. erecta was newly sequenced in this study. The total chloropalst genome size of T. erecta was 152,055 bp. In total, 123 genes were indetified, including 79 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. Twelve genes are containing introns (ycf3 and clpP contained two introns). The overall GC content of this genome was 37.4%. A further phylogenomic analysis of Asteraceae, including 23 taxa, was conducted for the placement of genus Tagetes. The complete plastome of T. erecta will provide a valuable resource for further genetic conservation, evolution, and molecular breading studies in Asteraceae.
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- 2019
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19. The complete mitochondrial genome of heartbreak grass Gelsemium elegans (Gardner & Champ.) Benth. (Gelsemiaceae)
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Yong-Hui Jin, Jun-Jie Wu, Yong-Qing Peng, Shu-Hang Hu, Hai-Shan Yan, Fan Xiao, Xuan Zhou, Jing Gao, Rui-Hong Wang, Ling Xu, and Zhe-Chen Qi
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gelsemium elegans ,mitochondrial genome ,phylogenetics ,toxic plant ,Genetics ,QH426-470 - Abstract
Gelsemium elegans, endemic to southern Asia, is a highly toxic plant with various medicinal functions. The complete mitochondrial genome of G. elegans was sequenced and assembled in this study. The genome is 405,990 bp in length and contains 37 protein-coding genes, 20 tRNA genes, and 3 rRNA genes. The phylogenetic tree based on 15 protein-coding genes common to six mitochondrial genomes in Gentianales support that G. elegans of Gelsemiaceae is sister to Apocynaceae.
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- 2019
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20. The complete chloroplast genome sequence of plumed cockscomb (Celosia argentea, Amaranthaceae)
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Ying-Xi Qian, Jing Gao, Yong-Hui Jin, Rui-Hong Wang, Ling Xu, and Zhe-Chen Qi
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celosia argentea ,chloroplast genome ,phylogenomics ,plumed cockscomb ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of Celosia argentea, an important horticultural and medicinal herb, was identified and sequenced in this study. The genome size is 153,474 bp, the GC content is 36.7%. A total of 123 genes were identified, including 84 protein-coding genes, 8 rRNA genes, and 33 tRNA genes. Twenty-nine plastome accessions from Caryophyllales were selected to assess the phylogenetic placement of genus and the result showed that C. argentea is most closely related to Amaranthus hypochondriacus.
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- 2019
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21. The complete chloroplast genome sequence of Chinese tallow Triadica sebifera (Linnaeus) Small (Euphorbiaceae)
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Xuan Zhou, Jun-Jie Wu, Jing Gao, Ying-Xi Qian, Cong Zhang, Ling Xu, Rui-Hong Wang, and Zhe-Chen Qi
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triadica sebifera ,chloroplast genome ,phylogeny ,Genetics ,QH426-470 - Abstract
Triadica sebifera, commonly known as Chinese tallow, is an important medicinal and biofuel plant native to eastern Asia. In the study, we assembled the complete chloroplast genome of T. sebifera based on Illumina pair-end sequencing data. The complete genome sequence length is 159,342 bp. Finally, 122 genes were identified, including 82 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. Further phylogenomic analysis showed that T. sebifera is clustered with Euphorbia esula.
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- 2019
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22. The complete chloroplast genome sequence of common Chinese ivy Hedera nepalensis var. sinensis (Araliaceae)
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Jun-Jie Wu, Xuan Zhou, Jing Gao, Yong-Qing Peng, Shu-Hang Hu, Ying-Xi Qian, Cong Zhang, Rui-Hong Wang, and Zhe-Chen Qi
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hedera nepalensis ,chloroplast genome ,araliaceae ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of widely cultivated Hedera nepalensis K. Koch var. sinensis (Tobl.) Rehder was sequenced and assembled in this study. The genome is 156,652 bp in length and contained 134 encoded genes in total, including 87 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. Based on available plastomes of 13 species in Araliaceae, we reconstructed their phylogenetic relationships and found that H. nepalensis var. sinensis is closely related to Fatsia japonica.
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- 2019
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23. Characterization of the complete chloroplast genome of the Chinese Kadsura vine Kadsura longipedunculata (Schisandraceae)
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Wen-Di Yu, Cong Zhang, Ying-Xi Qian, Jing Gao, Xue Wu, Meng-Di Li, Xuan Zhou, Jun-Jie Wu, Chao Shen, Rui-Hong Wang, and Zhe-Chen Qi
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chloroplast genome ,kadsura ,medicinal plant ,phylogenomics ,Genetics ,QH426-470 - Abstract
Kadsura (Schisandraceae) comprises ca. 16 species and widely distributes in eastern Asia. Many species in the genus were used as important medicinal plants in China and other Asian countries. In present study, we described the first Kadsura chloroplast genome, Kadsura longipedunculata, based on illumine pair-end sequencing data. The complete plastome length is 145,346 bp long with a large single copy, a small single copy, and two inverted repeat regions of length 94,947, 17,752, and 16,323 bp, respectively. A total of 118 genes were identified, including 75 protein-coding gens, 8 rRNA genes, and 34 tRNA genes. Eighteen plastome accessions from Austrobaileyales, Nymphaeales, and Magnoliales were selected to assess the phylogenetic placement of genus.
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- 2019
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24. The complete chloroplast genome sequence of Siphonostegia chinensis Benth. (Orobanchaceae)
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Jing Gao, Yong-Hui Jin, Ya-Qian Li, Ying-Xi Qian, Cong Zhang, Rui-Hong Wang, and Zhe-Chen Qi
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siphonostegia chinensis ,chloroplast genome ,phylogenomics ,Genetics ,QH426-470 - Abstract
Siphonostegia chinensis Benth. is a common Chinese herbal medicine widely distributed in eastern Asia. The complete chloroplast genome of S. chinensis was newly sequenced in this study. The total chloroplast genome size was 148,961 bp, its structure and gene contents were well conserved as typical chloroplast characteristics. The overall guanine-cytosine (GC) content of the S. chinensis chloroplast genome is 36.4%. The chloroplast genome encodes 130 unique genes, including 84 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. We used the cp genome of S. chinensis and 20 other chloroplast genomes to perform a phylogenetic analysis, which indicated that S. chinensis is sister to Schwalbea americana in Orobanchaceae.
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- 2019
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25. Characterization of the complete chloroplast genome of tara wingceltis (Pteroceltis tatarinowii, Ulmaceae)
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Xue Wu, Yong-Qing Peng, Shu-Hang Hu, Cong Zhang, Ying-Xi Qian, Meng-Di Li, Jun-Jie Wu, Xuan Zhou, Ling Xu, Rui-Hong Wang, and Zhe-Chen Qi
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chloroplast genome ,pteroceltis tatarinowii ,xuan paper ,Genetics ,QH426-470 - Abstract
Pteroceltis tatarinowii is a monotypic plant endemic to eastern China. It is an endangered tertiary relic and famous for Xuan paper manufacturing. In this study, we reported the Pteroceltis tatarinowii chloroplast genome with a length of 158,506 bp. In total, 132 genes were identified, including 83 protein-coding genes, 8 rRNA genes, and 33 tRNA genes. The overall GC content of this genome was 36.3%. Twenty-two species from Urticales were selected for phylogenomic analysis to determine the placement of P. tatarinowii. The complete plastome of P. tatarinowii will provide a valuable resource for further genetic conservation, phylogenomic, and evolution studies of the genus and the Ulmaceae.
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- 2019
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26. The complete chloroplast genome sequences of Barnardia japonica (Thunb.) Schult. and Schult.f
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Rui-Hong Wang, Jing Gao, Mengdi Li, Xue Wu, Chao Shen, Junjie Wu, Zhe-Chen Qi, and Pan Li
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asparagaceae ,barnardia ,chloroplast genome ,phylogenomics ,Genetics ,QH426-470 - Abstract
Barnardia japonica is an ornamental bulb with important medicinal usage. The complete chloroplast genome of B. japonica was newly sequenced in this study. The total chloroplast genome size of B. japonica was 156,129 bp. In total, 131 genes were identified, including 85 protein-coding genes, 8 rRNA genes, and 38 tRNA genes. Eighteen genes are containing introns (clpP and ycf3 contained two introns) and 18 genes had two copies. The overall GC content of this genome was 37.7%. A further phylogenomic analysis of Asparagales, including 36 taxa, was conducted for the placement of genus Barnardia. The complete plastome of B. japonica will provide a valuable resource for further genetic conservation, phylogenomic, and evolution studies in the genus and family.
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- 2018
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27. Characterization of the complete chloroplast genome of summer snowflake (Leucojum aestivum, Amaryllidaceae)
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Meng-Di Li, Xue Wu, Jun-Jie Wu, Xuan Zhou, Rui-Hong Wang, and Zhe-Chen Qi
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chloroplast genome ,leucojum aestivum ,medicinal plant ,phylogenomics ,Genetics ,QH426-470 - Abstract
Leucojum aestivum (Amaryllidaceae) is an important medicinal plant native to Europe, North Africa, and Central Asia. Its wild resources are Endangered because of excavation. In the present study, the chloroplast genome of L. aestivum was sequenced. The plastome length is 157,241 bp. A total of 132 genes were identified, consisting of 86 protein-coding genes, eight rRNA genes, and 38 tRNA genes. Thirty-four species from Asparagales were used for phylogenomic analysis.
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- 2018
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28. Characterization of the complete chloroplast genome of Chinese privet Ligustrum lucidum (Oleaceae)
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Rui-Hong Wang, Jing Gao, Meng-Di Li, Xue Wu, Chao Shen, Jun-Jie Wu, Ya-Qian Li, Yong-Hui Jin, Zhe-Chen Qi, and Zong-Suo Liang
- Subjects
oleaceae ,ligustrum ,chloroplast genome ,phylogenomics ,Genetics ,QH426-470 - Abstract
Ligustrum lucidum is a species of privet native to the southern half of China. It is often used as an ornamental tree, sometimes as a cultivar. In present study, we reported the Ligustrum lucidum chloroplast (cp) genome. The total chloroplast genome size of L. lucidum was 154,793 bp. In total, 124 genes were identified, including 81 protein-coding genes, 8 rRNA genes, and 35 tRNA genes. Twenty genes contained introns (clpP and ycf3 contained two introns) and 17 genes had two copies. The overall GC content of this genome was 38.2%. A further phylogenomic analysis of Oleaceae, including 21 taxa, was conducted for the placement of genus Ligustrum. The complete plastome of L. lucidum will provide a valuable resource for further genetic conservation, phylogenomic, and evolution studies of the genus and the family.
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- 2018
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29. The complete chloroplast genome of the Chinese Abelia Lineae chinensis (R.Br.) A.Braun & Vatke (Caprifoliaceae)
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Jin-Liang Liu, Ying-Xi Qian, Cong Zhang, Ya-Qian Li, Yong-Hui Jin, Xue Wu, Meng-Di Li, Jun-Jie Wu, Xuan Zhou, Chao Shen, Rui-Hong Wang, and Zhe-Chen Qi
- Subjects
lineae chinensis ,chloroplast genome ,phylogeny ,Genetics ,QH426-470 - Abstract
Linnaea chinensis (Caprifoliaceae), which inhabits in China and Japan, is commonly cultivated as an ornamental shrub. The complete chloroplast genome of L. chinensis was newly assembled in this study based on Illumina pair-end sequencing data. The full length of L. chinensis plastome is 155,813 bp. In total, 124 genes were identified, including 75 protein-coding genes, 8 rRNA genes, and 42 tRNA genes. The overall GC content of this genome was 38.4%. A further phylogenomic analysis including16 species from Adoxaceae and Caprifoliaceae was constructed.
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- 2018
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30. Characterization of the complete chloroplast genome of toad lily Tricyrtis Macropoda (Liliaceae)
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Rui-Hong Wang, Jing Gao, Xue Wu, Meng-Di Li, Chao Shen, Jun-Jie Wu, Wen-Di Yu, Jin-Liang Liu, Zhe-Chen Qi, and Zong-Suo Liang
- Subjects
chloroplast genome ,endangered ,phylogenomics ,tricyrtis ,Genetics ,QH426-470 - Abstract
Tricyrtis (Liliaceae) is an endemic genus in East Asia. Many of the species in the genus are in Endangered condition due to habitat loss and extensive horticultural usage in recent decades. In present study, we reported the first Tricyrtis chloroplast (cp) genome, Tricyrtis macropoda, based on Illumina pair-end sequencing data. The complete chloroplast genome size is 155,778 bp. In total, 131 genes were identified, including 85 protein-coding genes, 8 rRNA genes, and 38 tRNA genes. Fifteen genes are containing introns (clpP and ycf3 contained two introns) and 14 genes had two copies. The overall GC content of this genome was 37.4%. A further phylogenomic analysis of Liliales, including 62 taxa, was conducted for the placement of genus Tricyrtis. The complete plastome of T. marcropoda will provide a valuable resource for further genetic conservation, phylogenomic, and evolution studies in the genus and family.
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- 2018
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31. Effects of αB-crystallin on axon regeneration of rats retinal ganglion cells following acute ocular hypertension
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Rui-Hong Wang, Zhi-Hong Wu, Shi-Ke Hou, Hao-Jun Fan, and Li-Hua Wang
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αB-crystallin ,acute ocular hypertension ,retinal ganglion cell ,growth associated protein-43 ,electroretinogram ,Ophthalmology ,RE1-994 - Abstract
AIM: To study the effects of αB-crystallin on the αB-crystallin content in retina, growth associated protein-43(GAP-43)expression of retinal ganglion cells(RGCs), amplitude of electroretinogram(ERG)b-wave, and to investigate the promotive effect of αB-crystallin on axonal regeneration of RGCs after acute ocular hypertension(AOH). METHODS: One hundred and twenty male Sprague-Dawley(SD)rats(220±10g)were randomly divided into four groups(thirty in each group). αB group,(AOH following intravitreal injection of αB crystallin group, n=30). S group,(AOH following intravitreal injection of normal saline solution group, n=30). P group,(AOH following vitreous puncture group, n=30). H group,(AOH group, n=30). Western blot was assayed αB-crystallin content 7 and 14 days after model; Immunohistochemistry was observed GAP-43 expression 7, 14 and 21 days after model established; Amplitude of ERG b-wave was tested retinal function before and 1 month after model. RESULTS: The αB content in retina of αB group was higher 7 days after model established, fewer 14 days after model(P=0.000, P=0.000, P=0.001, P=0.000). But the αB content in retina of αB group was more serious compared with other groups at the same time points(P=0.019,P=0.016); The GAP-43 expression of RGCs in αB group was highest 7 days after model established, started to decrease 14 days after model established, which was fewest 21 days after model established(P=0.000, P=0.000, P=0.000, P=0.000). But the GAP-43 expression of RGCs in αB group was significantly higher than in other groups at each time point(P =0.002, P =0.011, P =0.009); The amplitude of ERG b-wave was lower 1 month after model(P=0.014, P=0.004, P=0.003, P=0.006). There were no statistical differences among groups on amplitude of ERG b-wave before model established(P=0.993). The amplitude of ERG b-wave in αB group was higher than others 1 month after model(P=0.002). CONCLUSION: Exogenous αB-crystallin could improve αB-crystallin expression of retinas; αB-crystallin contributed to the increase of GAP-43 expression of RGCs and RGCs axonal regeneration; αB-crystallin could promote the recovery of retinal function and was non-toxic on retinas of rat.
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- 2013
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32. Liver Steatosis and Increased ChREBP Expression in Mice Carrying a Liver Specific SIRT1 Null Mutation under a Normal Feeding Condition
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Rui-Hong Wang, Cuiling Li, Chu-Xia Deng
- Subjects
Biology (General) ,QH301-705.5 - Abstract
SIRT1, a homolog of yeast Sir2, is a type III NAD+ dependent histone and protein deacetylase. Previous studies of mice carrying liver specific deletion of exon 4 of the Sirt1 gene revealed opposite responses of mutant mice to a high-fat diet in terms of fatty liver formation, which obscures the function of SRIT1 in liver development and lipid metabolism. To investigate this, we deleted exons 5 and 6 of Sirt1 in the liver by using a Cre-loxP approach. Western blot using an antibody to N-terminal SIRT1 does not detect a truncated protein in the liver of the mutant mice (Sirt1flox5-6/flox5-6;Alb-Cre), suggesting a null mutation for SIRT1 is generated in the liver. Unlike the previously reported phenotypes, the Sirt1flox5-6/flox5-6;Alb-Cre mice develop fatty liver under a normal feeding condition. The disease starts at two months of age and incidence increases as the animals become older, affecting 78% of them when they are over one year of age. We showed that the steatosis is accompanied by altered expression of a number of genes, including increased expression of ChREBP, which acts as one of the central determinants of lipid synthesis in the liver. This data uncovers an important role of SIRT1 in regulating lipid metabolism in the liver, and the SIRT1 mutant mice may serve as an animal model for studying human fatty liver disease and facilitate the development of effective therapeutic approach for the disease.
- Published
- 2010
33. The population genetics of cultivation: domestication of a traditional Chinese medicine, Scrophularia ningpoensis Hemsl. (Scrophulariaceae).
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Chuan Chen, Pan Li, Rui-Hong Wang, Barbara A Schaal, and Cheng-Xin Fu
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Medicine ,Science - Abstract
BACKGROUND: Domestic cultivation of medicinal plants is an important strategy for protecting these species from over harvesting. Some species of medicinal plants have been brought into cultivation for more than hundreds years. Concerns about severe loss of genetic diversity and sustainable cultivation can potentially limit future use of these valuable plants. Genetic studies with comprehensive sampling of multiple medicinal species by molecular markers will allow for assessment and management of these species. Here we examine the population genetic consequences of cultivation and domestication in Scrophularia ningpoensis Hemsl. We used chloroplast DNA and genomic AFLP markers to clarify not only the effects of domestication on genetic diversity, but also determine the geographic origins of cultivars and their genetic divergence from native populations. These results will allow both better management of cultivated populations, but also provide insights for crop improvement. RESULTS: Twenty-one cpDNA haplotypes of S. ningpoensis were identified. Wild populations contain all haplotypes, whereas only three haplotypes were found in cultivated populations with wild populations having twice the haplotype diversity of cultivated populations. Genetic differentiation between cultivated populations and wild populations was significant. Genomic AFLP markers revealed similar genetic diversity patterns. Furthermore, Structure analysis grouped all wild populations into two gene pools; two of which shared the same gene pool with cultivated S. ningpoensis. The result of Neighbor-Joining analysis was consistent with the structure analysis. In principal coordinate analysis, three cultivated populations from Zhejiang Province grouped together and were separated from other cultivated populations. CONCLUSIONS: These results suggest that cultivated S. ningpoensis has experienced dramatic loss of genetic diversity under anthropogenic influence. We postulate that strong artificial selection for medicinal quality has resulted in genetic differentiation between cultivated and wild populations. Furthermore, it appears that wild populations in Jiangxi-Hunan area were involved in the origin of cultivated S. ningpoensis.
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- 2014
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34. Development of Microsatellite Loci in Scrophularia incisa (Scrophulariaceae) and Cross-Amplification in Congeneric Species
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Rui-Hong Wang, Chuan Chen, Qing Ma, Pan Li, and Cheng-Xin Fu
- Subjects
genetic diversity ,medicinal herb ,microsatellite ,Qinghai–Tibet Plateau ,Scrophularia dentata ,Scrophularia kiriloviana ,Biology (General) ,QH301-705.5 ,Botany ,QK1-989 - Abstract
Premise of the study: To elucidate the population genetics and phylogeography of Scrophularia incisa, microsatellite primers were developed. We also applied these microsatellite markers to its closely related species S. dentata and S. kiriloviana. Methods and Results: Using the compound microsatellite marker technique, 12 microsatellite primers were identified in S. incisa. The number of alleles ranged from 14 to 26 when assessed in 78 individuals from four populations. With high cross-species transferability, these primers also amplified in S. dentata and S. kiriloviana. Conclusions: These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure in the Chinese species of sect. Tomiophyllum at fine and range-wide geographical scales.
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- 2014
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35. The new portrait of mammary gland stem cells
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Rui-Hong Wang
- Subjects
Biology (General) ,QH301-705.5 - Published
- 2006
36. Supplementary Figure 1 from Absence of the Full-Length Breast Cancer–Associated Gene-1 Leads to Increased Expression of Insulin-Like Growth Factor Signaling Axis Members
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Chuxia Deng, Derek LeRoith, Shoshana Yakar, Sebastiano Andò, Xiaoling Xu, Cuiying Xiao, Rui-Hong Wang, Liu Cao, Xavier Coumoul, and Vivek Shukla
- Abstract
Supplementary Figure 1 from Absence of the Full-Length Breast Cancer–Associated Gene-1 Leads to Increased Expression of Insulin-Like Growth Factor Signaling Axis Members
- Published
- 2023
37. Data from Impaired Skin and Mammary Gland Development and Increased γ-Irradiation–Induced Tumorigenesis in Mice Carrying a Mutation of S1152-ATM Phosphorylation Site in Brca1
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Chu-Xia Deng, Kwan Ho Cho, Xiaoling Xu, Rui-Hong Wang, Cuiling Li, Sung-Chul Lim, Hye Jung Baek, Liu Cao, and Sang Soo Kim
- Abstract
The tumor suppressor BRCA1 interacts with many proteins and undergoes multiple modifications on DNA damage. ATM, a key molecule of the DNA damage response, phosphorylates S1189 of BRCA1 after γ-irradiation. S1189 of BRCA1 is known as a unique ATM phosphorylation site in BRCA1 exon 11. To study the functions of ATM-dependent phosphorylation of BRCA1-S1189, we generated a mouse model carrying a mutation of S1152A (S1152 in mouse Brca1 corresponds to S1189 in human BRCA1) by gene targeting. Brca1S1152A/S1152A mice were born at the expected ratio, unlike that seen in previous studies of Brca1-null mice. However, 36% of Brca1S1152A/S1152A mice exhibited aging-like phenotypes including growth retardation, skin abnormalities, and delay of the mammary gland morphogenesis, with an increase in apoptosis. Mutant mice were hypersensitive to high doses of γ-irradiation, displaying shortened life span and reduction in intestinal villus size, associated with increased apoptosis. Aging-unaffected 18-month-old Brca1S1152A/S1152A female mice also showed mammary gland abnormalities with increased levels of cyclin D1 and phospho-ER-α, such as Brca1-Δ11 mutation. On low-dose γ-irradiation, they suffered a marked increase in tumor formation with an abnormal coat pattern. Furthermore, Brca1S1152A/S1152A embryonic fibroblasts failed to accumulate p53 on γ-irradiation with delayed phosphorylation of p53-S23. These observations indicate that ATM-mediated phosphorylation of S1189 is required for BRCA1 functions in the modulation of DNA damage response and in the suppression of tumor formation by regulating p53 and apoptosis. [Cancer Res 2009;69(24):9291–300]
- Published
- 2023
38. Data from Absence of the Full-Length Breast Cancer–Associated Gene-1 Leads to Increased Expression of Insulin-Like Growth Factor Signaling Axis Members
- Author
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Chuxia Deng, Derek LeRoith, Shoshana Yakar, Sebastiano Andò, Xiaoling Xu, Cuiying Xiao, Rui-Hong Wang, Liu Cao, Xavier Coumoul, and Vivek Shukla
- Abstract
The breast cancer–associated gene-1 (BRCA1) plays many important functions in multiple biological processes/pathways. Mice homozygous for a targeted deletion of full-length BRCA1 (Brca1Δ11/Δ11) display both increased tumorigenesis and premature aging, yet molecular mechanisms underlying these defects remain elusive. Here, we show that Brca1 deficiency leads to increased expression of several insulin-like growth factor (IGF) signaling axis members in multiple experimental systems, including BRCA1-deficient mice, primary mammary tumors, and cultured human cells. Furthermore, we provide evidence that activation of IGF signaling by BRCA1 deficiency can also occur in a p53-independent fashion. Our data indicate that BRCA1 interacts with the IRS-1 promoter and inhibits its activity that is associated with epigenetic modification of histone H3 and histone H4 to a transcriptional repression chromatin configuration. We further show that BRCA1-deficient mammary tumor cells exhibit high levels of IRS-1, and acute suppression of Irs-1 using RNA interference significantly inhibits growth of these cells. Those observations provide a molecular insight in understanding both fundamental and therapeutic BRCA1-associated tumorigenesis and aging. (Cancer Res 2006; 66(14): 7151-7)
- Published
- 2023
39. Supplementary Methods and Figure 1 from Impaired Skin and Mammary Gland Development and Increased γ-Irradiation–Induced Tumorigenesis in Mice Carrying a Mutation of S1152-ATM Phosphorylation Site in Brca1
- Author
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Chu-Xia Deng, Kwan Ho Cho, Xiaoling Xu, Rui-Hong Wang, Cuiling Li, Sung-Chul Lim, Hye Jung Baek, Liu Cao, and Sang Soo Kim
- Abstract
Supplementary Methods and Figure 1 from Impaired Skin and Mammary Gland Development and Increased γ-Irradiation–Induced Tumorigenesis in Mice Carrying a Mutation of S1152-ATM Phosphorylation Site in Brca1
- Published
- 2023
40. The application of PCA in the basic public service system.
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Juan Wang, Rui-Hong Wang, Jian-Guo Shi, and Yan-Jie Liu
- Published
- 2012
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41. Coal mine reconfigurable monitoring model based on Wireless Sensor Network.
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Deng Pan Zhang, Rui Hong Wang, and Jian Fa Cai
- Published
- 2011
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42. Microstructure and mechanical properties of lanthanum oxide-doped Mo–12Si–8.5B(at%) alloys
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Guo-jun, Zhang, Qian, Dang, Hao, Kou, Rui-hong, Wang, Gang, Liu, and Jun, Sun
- Published
- 2013
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43. Magnetic Properties and Crystal Structure of (Fe,Ni)2( P,Si) Quaternaries of Fe2P-Type
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Rui Hong Wang, Tian Yi Ren, and Tian Liang Wang
- Subjects
Materials science ,Condensed matter physics ,Mechanics of Materials ,Mechanical Engineering ,Curie temperature ,General Materials Science ,Crystal structure ,Type (model theory) ,Condensed Matter Physics ,Magnetocrystalline anisotropy - Abstract
(Fe,Ni)2(P,Si) compounds were synthesized and characterized. Ni substitution in Fe1.95-xNixP0.7Si0.3 is found to favor the formation of Fe2P-type hexagonal structure. The samples appear nearly single phase. Powder oriented in the magnetic field shows a pronounced uniaxial magnetic anisotropy with c axis as the easy axis. Magnetization measurements carried out along and perpendicular to the c crystal axis demonstrate a significant magnetic anisotropy, making these materials potential candidates for permanent magnet applications. We found that (Fe,Ni)2(P,Si) compound has no remanent magnetic field and coercivity, but it has a large magnetocrystalline anisotropy at room temperature. Therefore, doping Fe2P type compounds with a small amount of Ni and Si may be a promising way to create new materials with large magnetocrystalline anisotropy at room temperature, and thus rare-earth free permanent magnet.
- Published
- 2020
44. Development of 19 novel microsatellite markers of lily-of-the-valley (Convallaria, Asparagaceae) from transcriptome sequencing
- Author
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Rui-Hong Wang, Jing Gao, Pan Li, Xuan Zhou, Xue Wu, Qi-Xiang Lu, Zhe-Chen Qi, Jun-Jie Wu, Yu-Kun Wei, and Meng-Di Li
- Subjects
0301 basic medicine ,Heterozygote ,food.ingredient ,Population ,Lily of the valley ,Convallaria ,Population genetics ,03 medical and health sciences ,0302 clinical medicine ,Asparagaceae ,food ,Exome Sequencing ,Botany ,Genetics ,education ,Molecular Biology ,Alleles ,education.field_of_study ,Genetic diversity ,Polymorphism, Genetic ,biology ,Genetic Variation ,High-Throughput Nucleotide Sequencing ,General Medicine ,biology.organism_classification ,Genetics, Population ,030104 developmental biology ,Genetic Loci ,030220 oncology & carcinogenesis ,Genetic structure ,Microsatellite ,Transcriptome ,Microsatellite Repeats - Abstract
The lily-of-the-valley Convallaria (Asparagaceae) consists of three herbaceous perennial species. The plants are commonly found in northern hemisphere, and are best-known for their ornamental and pharmaceutical value. In order to assess the genetic structure, diversity and demographic history of Convallaria species, 19 novel microsatellite markers were developed based on transcriptome data of C. keiskei. Polymorphism and cross-amplification of the markers were tested in three populations of C. keiskei and one population each of C. majalis and C. montana. The transferability rate in two species was both 89.5%. The average number of alleles detected per locus was 7.7, 3.3 and 2.7 in C. keiskei, C. majalis and C. montana, respectively, and the polymorphism information content correspondingly varied from 0.067 to 0.730, from 0.071 to 0.637 and from 0.195 to 0.680 at the population level. The observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.833, respectively. Seven of the 19 loci showed significant deviation from Hardy-Weinberg equilibrium. The availability of these markers will provide a useful molecular tool for further population genetics, phylogeographic and breeding studies of Convallaria species.
- Published
- 2020
45. Tensile properties and strengthening mechanisms of Mo–Si alloy
- Author
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Guo-jun, Zhang, Xiao-hui, Lin, Rui-hong, Wang, Gang, Liu, and Jun, Sun
- Published
- 2011
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46. The efficacy and safety of alpha-adrenergic blockers for medical expulsion therapy in patients with ureteral calculi
- Author
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Rui-Hong Wang, Ze-Wei Yu, Chang-Cun Zhang, and Jian-Gang Gao
- Subjects
Retrograde ejaculation ,medicine.medical_specialty ,ureteral calculi ,alpha adrenergic blocker ,Urology ,Cochrane Library ,Placebo ,Placebos ,Terazosin ,Tamsulosin ,Odds Ratio ,medicine ,Humans ,Adverse effect ,Alfuzosin ,Adrenergic alpha-Antagonists ,business.industry ,urolithiasis ,General Medicine ,Silodosin ,medicine.disease ,medical expulsion therapy ,meta-analysis ,Treatment Outcome ,business ,Systematic Review and Meta-Analysis ,medicine.drug ,Research Article - Abstract
Purpose: Alpha-adrenergic blockers are commonly used as a medical expulsive therapy (MET) for patients with ureteral calculi. The aim of this meta-analysis was to evaluate the efficacy and safety of alpha-adrenergic blockers compared with a placebo when used as a MET. Materials and methods: We carried out a systematic search of the PubMed, EMBASE, and Web of Science databases, and the Cochrane Library, for relevant articles from inception to November 2020. Our aim was to identify placebo-controlled trails in which patients were randomized to receive either alpha-adrenergic blockers (tamsulosin, alfuzosin, doxazosin, terazosin, naftopidil, or silodosin) or a placebo for the treatment of ureteral calculi. Results: According to strict inclusion criteria, database searches identified 8 placebo-controlled studies that included 2284 patients. Generally, α-blockers had no significant effect on the clearance of stones in the urinary tract (risk ratio [RR] = 1.05; 95% confidence interval [CI] = 1.00–1.11). However, subgroup analysis showed that α-blockers were effective in treating distal urinary tract stones (RR = 1.08; 95% CI = 1.02–1.15). With regards to adverse events, our analysis showed that the combination of MET with α-blockers was likely to cause dizziness (RR = 1.37; 95% CI = 1.06–1.79) and retrograde ejaculation (RR = 3.10; 95% CI = 1.81–5.29). Conclusion: Although α-blockers cannot improve the overall ureteral stone clearance rate, these drugs are still effective for the treatment of stones in the distal urinary tract. However, the application of α-blockers is likely to cause dizziness and/or retrograde ejaculation.
- Published
- 2021
47. Definition of a new blood cell count (BCT) score for early survival prediction for non-small cell lung cancer patients treated with atezolizumab: Integrated analysis of 4 multicenter clinical trials
- Author
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Jian-Guo Zhou, Udo S. Gaipl, Rui-Hong Wang, Su-Han Jin, Fangya Tan, Shamus R. Carr, David S. Schrump, Hu Ma, Bo Shen, Markus Hecht, Si-si He, Rainer Fietkau, Ada Hang-Heng Wong, Benjamin Frey, Yu-Zhong Chen, Haitao Wang, and Gang Shen
- Subjects
Oncology ,medicine.medical_specialty ,Proportional hazards model ,business.industry ,Immune checkpoint inhibitors ,medicine.disease ,Clinical trial ,Blood cell ,medicine.anatomical_structure ,Docetaxel ,Atezolizumab ,Internal medicine ,medicine ,Non small cell ,Lung cancer ,business ,medicine.drug - Abstract
IntroductionImmune checkpoint inhibitor (ICI) therapy is a major breakthrough in non-small cell lung cancer (NSCLC) treatment. However, valid predictive biomarkers are lacking. Blood cell count test (BCT) provides a direct quantification of various types of immune cells (ICs) to reveal the immune landscape to predict ICI treatment.MethodsThis study analyzed four international, multi-center clinical trials (OAK, BIRCH, POPLAR and FIR trials) to conduct post-hoc analyses of NSCLC patients undergoing atezolizumab (anti-PD-L1) single-agent treatment (n = 1,479) or docetaxel single-agent treatment (n = 707). BCT was conducted at three timepoints: pre-treatment (T1), the first day of treatment cycle 3 (T2), and first day of treatment cycle 5 (T3). Univariate and multivariate Cox regression analyses were conducted to identify early BCT biomarkers to predict atezolizumab treatment outcomes in NSCLC patients.ResultsThe BCT biomarkers of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) at timepoint T3 and neutrophil-to-monocyte ratio (NMR) at timepoint T2 were identified as strong predictive biomarkers for atezolizumab (Ate)-treated NSCLC patients in comparison to docetaxel (Dtx)-treated patients regarding overall survival (OS) (BCTscore low-risk: HR Ate vs Dtx = 1.54 (95% CI: 1.04-2.27), P = 0.036; high-risk: HR Ate vs Dtx = 0.84 (95% CI: 0.62-1.12), P = 0.236). This identified BCTscore model showed better OS AUC in the OAK (AUC12month=0.696), BIRCH (AUC12month=0.672) and POPLAR+FIR studies (AUC12month=0.727) than that of each of the three single BCT biomarkers.ConclusionThe BCTscore model is a valid predictive and prognostic biomarker for atezolizumab-treated NSCLC patients.
- Published
- 2021
48. Sirt1 deficiency upregulates glutathione metabolism to prevent hepatocellular carcinoma initiation in mice
- Author
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Xiaoling Xu, Chu-Xia Deng, Haitao Wang, Weiping Chen, Samson Sek Man Su, Shaowei Wang, Kimmy Ka Wing Lei, Katherine Perkins Prothro, David S. Schrump, Rui-Hong Wang, Lakhansing Pardeshi, Qiang Chen, Pengxiang Qiu, Weilong Hou, and Yashvita Shukla
- Subjects
Male ,Cancer Research ,Biology ,Article ,chemistry.chemical_compound ,Mice ,Liver Neoplasms, Experimental ,Sirtuin 1 ,Genetics ,medicine ,Animals ,Molecular Biology ,Gene ,Fatty acid synthesis ,Mice, Knockout ,Glutathione ,medicine.disease ,digestive system diseases ,Up-Regulation ,Metabolic pathway ,chemistry ,Gluconeogenesis ,Hepatocellular carcinoma ,Cancer research ,Liver cancer ,Cysteine - Abstract
Sirtuin-1 (SIRT1) is involved in various metabolic pathways, including fatty acid synthesis and gluconeogenesis in the liver. However, its role in initiation and progression of liver cancer remains unclear. Studying Sirt1 liver-specific knockout (LKO) mice in combination with diethylnitrosamine (DEN) treatment, we demonstrated that loss of Sirt1 rendered mice resistant to DEN-induced hepatocellular carcinoma (HCC) development. RNA-seq revealed that livers from LKO mice exhibited an enrichment in glutathione metabolism eight months after DEN challenge. Sirt1 deficiency elevated the expression of glutathione-s-transferase family genes by increasing the level of Nrf2, a key regulator of glutathione metabolism. Hence, LKO livers displayed a reductive environment with an increased ratio of GSH to GSSG and an elevated GSH level. Furthermore, using CRISPR knockout techniques, we confirmed that the impairment of HCC formation in LKO mice is mainly dependent on NRF2 signaling. Meanwhile, HCC induced by DEN could be blocked by the administration of N-acetyl cysteine (NAC) when administered one month after DEN challenge. However, NAC treatment starting five months after DEN injection was not able to prevent tumor development. In conclusion, our findings indicate that a reductive environment orchestrated by glutathione metabolism at an early stage can prevent the initiation of HCC.
- Published
- 2021
49. Super-Dispersed Fe–N Sites Embedded into Porous Graphitic Carbon for ORR: Size, Composition and Activity Control
- Author
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Rui Hong Wang, Jian Cong Liu, Yan Qing Jiao, Xin Yu Wang, and Ze Wei Lin
- Subjects
high catalytic performance ,Materials science ,ORR ,Carbonization ,General Chemical Engineering ,Electrolyte ,Article ,Catalysis ,Chemistry ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Imidazolate ,Graphitic carbon ,General Materials Science ,Fe-N sites ,Methanol ,super-dispersion ,Porosity ,QD1-999 ,ZIF-8 ,Zeolitic imidazolate framework - Abstract
Searching for high-efficient, good long-term stability, and low-cost electrocatalysts toward oxygen reduction reaction (ORR) is highly desirable for the development of sustainable energy conversion devices. Iron–nitrogen doped carbon (Fe–N/C) catalysts have been recognized as the most promising candidates for traditional Pt-based catalysts that benefit from their high activity, excellent anti-poisoning ability, and inexpensiveness. Here, a super-dispersed and high-performance Fe–N/C catalyst was derived from chemically Fe-doped zeolitic imidazolate frameworks (ZIFs) by directly bonding Fe ions to imidazolate ligands within 3D frameworks. It produced a series of catalysts, whose sizes could be tuned in the range from 62 to over 473 nm in diameter. After rationally regulating the component and heating treatment, the best ORR activity was measured for the catalyst with a size of 105 nm, which was obtained when the Fe3+/Zn2+ molar ratio was 0.05 and carbonization temperature was 900 °C. It exhibited a high onset potential (Eonset = 0.99 V) and half-wave potential (E1/2 = 0.885 V) compared with a commercial 20% Pt/C catalyst (Eonset = 0.10 V, E1/2 = 0.861 V) as well as much better durability and methanol resistance in an alkaline electrolyte.
- Published
- 2021
50. Evolutionary Comparison of the Complete Chloroplast Genomes in Convallaria Species and Phylogenetic Study of Asparagaceae
- Author
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Qi-Xiang Lu, Xiao Chang, Jing Gao, Xue Wu, Jing Wu, Zhe-Chen Qi, Rui-Hong Wang, Xiao-Ling Yan, and Pan Li
- Subjects
RNA, Transfer ,Genetics ,Genome, Chloroplast ,Convallaria ,chloroplast genome ,comparative analysis ,phylogenomics ,Asparagaceae ,Phylogeny ,Genetics (clinical) - Abstract
The genusiConvallaria/i(Asparagaceae) comprises three herbaceous perennial species that are widely distributed in the understory of temperate deciduous forests in the Northern Hemisphere. AlthoughiConvallaria/ispecies have high medicinal and horticultural values, studies related to the phylogenetic analysis of this genus are few. In the present study, we assembled and reported five complete chloroplast (cp) sequences of threeiConvallaria/ispecies (two ofiC. keiskei/iMiq., two ofiC. majalis/iL., and one ofiC. montana/iRaf.) using Illumina paired-end sequencing data. The cp genomes were highly similar in overall size (161,365-162,972 bp), and all consisted of a pair of inverted repeats (IR) regions (29,140-29,486 bp) separated by a large single-copy (LSC) (85,183-85,521 bp) and a small single-copy (SSC) region (17,877-18,502 bp). Each cp genome contained the same 113 unique genes, including 78 protein-coding genes, 30 transfer RNA genes, and 4 ribosomal RNA genes. Gene content, gene order, AT content and IR/SC boundary structure were nearly identical among all of theiConvallaria/icp genomes. However, their lengths varied due to contraction/expansion at the IR/LSC borders. Simple sequence repeat (SSR) analyses indicated that the richest SSRs are A/T mononucleotides. Three highly variable regions (ipetA/i-ipsbJ/i,ipsbI/i-itrnS/iandiccsA/i-indhD/i) were identified as valuable molecular markers. Phylogenetic analysis of the family Asparagaceae using 48 cp genome sequences supported the monophyly ofiConvallaria/i, which formed a sister clade to the genusiRohdea/i. Our study provides a robust phylogeny of the Asparagaceae family. The complete cp genome sequences will contribute to further studies in the molecular identification, genetic diversity, and phylogeny ofiConvallaria/i.
- Published
- 2022
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