2,813 results on '"Rui Cheng"'
Search Results
2. Genome-wide characterization of DELLA gene family in blueberry (Vaccinium darrowii) and their expression profiles in development and response to abiotic stress
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Houjun Zhou, Yanwen Wang, Xinyu Wang, Rui Cheng, Hongxia Zhang, and Lei Yang
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Blueberry ,Expression profiles ,Gene family ,DELLA ,Development ,Abiotic stress ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The DELLA proteins, a class of GA signaling repressors, belong to the GRAS family of plant-specific nuclear proteins. Members of DELLA gene family encode transcriptional regulators with diverse functions in plant development and abiotic stress responses. To date, DELLAs have been identified in various plant species, such as Arabidopsis thaliana, Malus domestica, Populus trichocarpa, and other land plants. Most information of DELLA family genes was obtained from A. thaliana, whereas little is known about the DELLA gene family in blueberry. Results In this study, we identified three DELLA genes in blueberry (Vaccinium darrowii, VdDELLA) and provided a complete overview of VdDELLA gene family, describing chromosome localization, protein properties, conserved domain, motif organization, and phylogenetic analysis. Three VdDELLA members, containing two highly conserved DELLA domain and GRAS domain, were distributed across three chromosomes. Additionally, cis-acting elements analysis indicated that VdDELLA genes might play a critical role in blueberry developmental processes, hormone, and stress responses. Expression analysis using quantitative real-time PCR (qRT-PCR) revealed that all of three VdDELLA genes were differentially expressed across various tissues. VdDELLA2 was the most highly expressed VdDELLA in all denoted tissues, with a highest expression in mature fruits. In addition, all of the three VdDELLA genes actively responded to diverse abiotic stresses. Based on qRT-PCR analysis, VdDELLA2 might act as a key regulator in V. darrowii in response to salt stress, whereas VdDELLA1 and VdDELLA2 might play an essential role in cold stress response. Under drought stress, all of three VdDELLA genes were involved in mediating drought response. Furthermore, their transiently co-localization with nuclear markers in A. thaliana protoplasts demonstrated their transcriptional regulator roles. Conclusions In this study, three VdDELLA genes were identified in V. darrowii genome. Three VdDELLA genes were closely related to the C. moschata DELLA genes, S. lycopersicum DELLA genes, and M. domestica DELLA genes, respectively, indicating their similar biological functions. Expression analysis indicated that VdDELLA genes were highly efficient in blueberry fruit development. Expression patterns under different stress conditions revealed the differentially expressed VdDELLA genes responding to salt, drought, and cold stress. Overall, these results enrich our understanding of evolutionary relationship and potential functions of VdDELLA genes, which provide valuable information for further studies on genetic improvement of the plant yield and plant resistance.
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- 2024
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3. Trends in the antimicrobial susceptibility among Chinese neonates from 2012 to 2021: a multicenter study
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Zhanghua Yin, Jintong Tan, Huafei Huang, Jianyuan Zhao, Xiaohui Gong, Jing Li, Chao Chen, Fei Luo, Xiaoyi Huang, Huaiyan Wang, Hongyan Lu, Mingfu Wu, Renqiang Yu, Xiaoping Lei, Qian Zhang, Fengdan Xu, Ning Li, Hong Jiang, Jianhua Fu, Rui Cheng, Yan Chen, and Yongjun Zhang
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Full-term neonates ,Invasive bacterial infections ,Antimicrobial susceptibility ,Minimum inhibitory concentration ,Trends ,China ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Antibiotic resistance is a serious global public health issue. However, there are few reports on trends in antimicrobial susceptibility in Chinese neonates, and most of the existing evidence has been derived from adult studies. We aimed to assess the trends in antimicrobial susceptibility of common pathogens in full-term neonates with invasive bacterial infections (IBIs) in China. Methods This cross-sectional survey study analyzed the antimicrobial susceptibility in Chinese neonates with IBIs from 17 hospitals, spanning from January 2012 to December 2021. Joinpoint regression model was applied to illustrate the trends and calculate the average annual percentage change (AAPC). Using Mantel-Haenszel linear-by-linear association chi-square test, we further compared the antibiotic minimum inhibitory concentrations (MICs) by pathogens between 2019 and 2021 to provide precise estimates of changes. Results The proportion of Escherichia coli with extended-spectrum-beta-lactamase-negative strains increased from 0.0 to 88.5% (AAPC = 62.4%, 95% confidence interval (CI): 44.3%, 82.9%), with two breakpoints in 2014 and 2018 (p-trend
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- 2024
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4. Differentiation of glioma and solitary brain metastasis: a multi-parameter magnetic resonance imaging study using histogram analysis
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Yifei Su, Rui Cheng, Jinxia Guo, Miaoqi Zhang, Junhao Wang, Hongming Ji, Chunhong Wang, Liangliang Hao, Yexin He, and Cheng Xu
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Intravoxel incoherent motion ,Amide proton transfer-weighted imaging ,Glioma ,Solitary brain metastasis ,MRI ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Differentiation of glioma and solitary brain metastasis (SBM), which requires biopsy or multi-disciplinary diagnosis, remains sophisticated clinically. Histogram analysis of MR diffusion or molecular imaging hasn’t been fully investigated for the differentiation and may have the potential to improve it. Methods A total of 65 patients with newly diagnosed glioma or metastases were enrolled. All patients underwent DWI, IVIM, and APTW, as well as the T1W, T2W, T2FLAIR, and contrast-enhanced T1W imaging. The histogram features of apparent diffusion coefficient (ADC) from DWI, slow diffusion coefficient (Dslow), perfusion fraction (frac), fast diffusion coefficient (Dfast) from IVIM, and MTRasym@3.5ppm from APTWI were extracted from the tumor parenchyma and compared between glioma and SBM. Parameters with significant differences were analyzed with the logistics regression and receiver operator curves to explore the optimal model and compare the differentiation performance. Results Higher ADCkurtosis (P = 0.022), frackurtosis (P
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- 2024
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5. High-brightness betatron emission from the interaction of a sub picosecond laser pulse with pre-ionized low-density polymer foam for ICF research
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Mikhail Gyrdymov, Jakub Cikhardt, Parysatis Tavana, Nataliya G. Borisenko, Sergey Yu. Gus´kov, Rafael A. Yakhin, Galina A. Vegunova, Wenqing Wei, Jieru Ren, Yongtao Zhao, Dieter H. H. Hoffmann, Zhigang Deng, Weimin Zhou, Rui Cheng, Jie Yang, Jan Novotny, Xiaofei Shen, Alexander Pukhov, Joachim Jacoby, Christian Spielmann, Viacheslav S. Popov, Mikhail E. Veysman, Nikolay E. Andreev, and Olga N. Rosmej
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Medicine ,Science - Abstract
Abstract Direct laser acceleration (DLA) of electrons in plasmas of near-critical density (NCD) is a very advancing platform for high-energy PW-class lasers of moderate relativistic intensity supporting Inertial Confinement Fusion research. Experiments conducted at the PHELIX sub-PW Nd:glass laser demonstrated application-promising characteristics of DLA-based radiation and particle sources, such as ultra-high number, high directionality and high conversion efficiency. In this context, the bright synchrotron-like (betatron) radiation of DLA electrons, which arises from the interaction of a sub-ps PHELIX laser pulse with an intensity of 1019 W/cm2 with pre-ionized low-density polymer foam, was studied. The experimental results show that the betatron radiation produced by DLA electrons in NCD plasma is well directed with a half-angle of 100–200 mrad, yielding (3.4 ± 0.4)·1010 photons/keV/sr at 10 keV photon energy. The experimental photon fluence and the brilliance agree well with the particle-in-cell simulations. These results pave the way for innovative applications of the DLA regime using low-density pre-ionized foams in high energy density research.
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- 2024
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6. Real-time predictive model of extrauterine growth retardation in preterm infants with gestational age less than 32 weeks
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Liang Gao, Wei Shen, Fan Wu, Jian Mao, Ling Liu, Yan-Mei Chang, Rong Zhang, Xiu-Zhen Ye, Yin-Ping Qiu, Li Ma, Rui Cheng, Hui Wu, Dong-Mei Chen, Ling Chen, Ping Xu, Hua Mei, San-Nan Wang, Fa-Lin Xu, Rong Ju, Zhi Zheng, Xin-Zhu Lin, Xiao-Mei Tong, and The Chinese Multicenter EUGR Collaborative Group
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Very preterm infant ,Extrauterine growth retardation ,Prediction ,Nomogram ,Medicine ,Science - Abstract
Abstract The aim of this study was to develop a real-time risk prediction model for extrauterine growth retardation (EUGR). A total of 2514 very preterm infants were allocated into a training set and an external validation set. The most appropriate independent variables were screened using univariate analysis and Lasso regression with tenfold cross-validation, while the prediction model was designed using binary multivariate logistic regression. A visualization of the risk variables was created using a nomogram, while the calibration plot and receiver operating characteristic (ROC) curves were used to calibrate the prediction model. Clinical efficacy was assessed using the decision curve analysis (DCA) curves. Eight optimal predictors that namely birth weight, small for gestation age (SGA), hypertensive disease complicating pregnancy (HDCP), gestational diabetes mellitus (GDM), multiple births, cumulative duration of fasting, growth velocity and postnatal corticosteroids were introduced into the logistic regression equation to construct the EUGR prediction model. The area under the ROC curve of the training set and the external verification set was 83.1% and 84.6%, respectively. The calibration curve indicate that the model fits well. The DCA curve shows that the risk threshold for clinical application is 0–95% in both set. Introducing Birth weight, SGA, HDCP, GDM, Multiple births, Cumulative duration of fasting, Growth velocity and Postnatal corticosteroids into the nomogram increased its usefulness for predicting EUGR risk in very preterm infants.
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- 2024
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7. Mussel-inspired immunomodulatory and osteoinductive dual-functional hydroxyapatite nanoplatform for promoting bone regeneration
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Danlei Qin, Yifan Zhao, Rui Cheng, Yingyu Liu, Susu Guo, Lingxiang Sun, Yanqin Guo, Fengxiang Hao, and Bin Zhao
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Mussel-inspired nanocomposites ,Bone repair ,Immunomodulation ,Osteoinductivity ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Simultaneously modulating the inflammatory microenvironment and promoting local bone regeneration is one of the main challenges in treating bone defects. In recent years, osteoimmunology has revealed that the immune system plays an essential regulatory role in bone regeneration and that macrophages are critical components. In this work, a mussel-inspired immunomodulatory and osteoinductive dual-functional hydroxyapatite nano platform (Gold/hydroxyapatite nanocomposites functionalized with polydopamine - PDA@Au-HA) is developed to accelerate bone tissues regeneration by regulating the immune microenvironment. PDA coating endows nanomaterials with the ability to scavenge reactive oxygen species (ROS) and anti-inflammatory properties, and it also exhibits an immunomodulatory ability to inhibit M1 macrophage polarization and activate M2 macrophage secretion of osteogenesis-related cytokines. Most importantly, this nano platform promotes the polarization of M2 macrophages and regulates the crosstalk between macrophages and pre-osteoblast cells to achieve bone regeneration. Au-HA can synergistically promote vascularized bone regeneration through sustained release of Ca and P particles and gold nanoparticles (NPs). This nano platform has a synergistic effect of good compatibility, scavenging of ROS, and anti-inflammatory and immunomodulatory capability to accelerate the bone repair process. Thus, our research offers a possible therapeutic approach by exploring PDA@Au-HA nanocomposites as a bifunctional platform for tissue regeneration.
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- 2024
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8. Genome-wide prediction and functional analysis of WOX genes in blueberry
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Yanwen Wang, Lei Yang, Wenzhu Geng, Rui Cheng, Hongxia Zhang, and Houjun Zhou
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Blueberry ,Transcription factor ,Bioinformatics ,Tissue-specific expression ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background WOX genes are a class of plant-specific transcription factors. The WUSCHEL-related homeobox (WOX) family is a member of the homeobox transcription factor superfamily. Previous studies have shown that WOX members play important roles in plant growth and development. However, studies of the WOX gene family in blueberry plants have not been reported. Results In order to understand the biological function of the WOX gene family in blueberries, bioinformatics were used methods to identify WOX gene family members in the blueberry genome, and analyzed the basic physical and chemical properties, gene structure, gene motifs, promoter cis-acting elements, chromosome location, evolutionary relationships, expression pattern of these family members and predicted their functions. Finally, 12 genes containing the WOX domain were identified and found to be distributed on eight chromosomes. Phylogenetic tree analysis showed that the blueberry WOX gene family had three major branches: ancient branch, middle branch, and WUS branch. Blueberry WOX gene family protein sequences differ in amino acid number, molecular weight, isoelectric point and hydrophobicity. Predictive analysis of promoter cis-acting elements showed that the promoters of the VdWOX genes contained abundant light response, hormone, and stress response elements. The VdWOX genes were induced to express in both stems and leaves in response to salt and drought stress. Conclusions Our results provided comprehensive characteristics of the WOX gene family and important clues for further exploration of its role in the growth, development and resistance to various stress in blueberry plants.
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- 2024
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9. A large-scale multi-attribute group decision-making method with R-numbers and its application to hydrogen fuel cell logistics path selection
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Rui Cheng, Jianping Fan, Meiqin Wu, and Hamidreza Seiti
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R-numbers ,LSMAGDM ,Consensus researching ,LOPCOW ,CRADIS ,Electronic computers. Computer science ,QA75.5-76.95 ,Information technology ,T58.5-58.64 - Abstract
Abstract The large-scale multi-attribute group decision-making (LSMAGDM) problem has become a hot research topic in the field of decision science. An R-numbers large-scale multi-attribute group decision-making (R-LSMAGDM) model is proposed to be constructed in this paper based on the advantages of R-numbers in capturing risks. First, the most commonly used clustering method, k-means, is introduced to determine the sub-groups. Then, a new sub-group weighting determination model is constructed by considering sub-group size and sub-group entropy. Next, we also build an optimized consensus-reaching model by improving the calculation method of the mean value. Then, the R-numbers weighted Hamy mean (RNWHM) operator is proposed to aggregate the sub-group information. In addition, the logarithmic percentage change-driven objective weighting (LOPCOW) method and the compromise ranking of alternatives from distance to ideal solution (CRADIS) method are used for attribute weighting calculation and alternative ranking, respectively. Finally, the effectiveness of the model is verified by an application example of hydrogen fuel cell logistics path selection.
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- 2024
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10. The current clinical landscape of neonatal respiratory failure in Jiangsu Province of China: patient demographics, NICU treatment interventions, and patient outcomes
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Na Wang, Ke-Yu Lu, Shan-Yu Jiang, Hong-Wei Wu, Rui Cheng, Zhao-Jun Pan, Huai-Yan Wang, and on behalf of the Jiangsu Provincial Collaborative Study Group for Neonatal Respiratory Diseases
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Neonatology ,Respiratory failure ,Morbidity ,Mortality ,Neonatal intensive care ,Pediatrics ,RJ1-570 - Abstract
Abstract Introduction Neonatal respiratory failure (NRF) is a serious condition that often has high mortality and morbidity, effective interventions can be delivered in the future by identifying the risk factors associated with morbidity and mortality. However, recent advances in respiratory support have improved neonatal intensive care units (NICUs) care in China. We aimed to provide an updated review of the clinical profile and outcomes of NRF in the Jiangsu province. Methods Infants treated for NRF in the NICUs of 28 hospitals between March 2019 and March 2022 were retrospectively reviewed. Data collected included baseline perinatal and neonatal parameters, NICU admission- and treatment-related data, and patient outcomes in terms of mortality, major morbidity, and survival without major morbidities. Results A total of 5548 infants with NRF were included in the study. The most common primary respiratory disorder was respiratory distress syndrome (78.5%). NRF was managed with non-invasive and invasive respiratory support in 59.8% and 14.5% of patients, respectively. The application rate of surfactant therapy was 38.5%, while that of neonatal extracorporeal membrane oxygenation therapy was 0.2%. Mortality and major morbidity rates of 8.5% and 23.2% were observed, respectively. Congenital anomalies, hypoxic-ischemic encephalopathy, invasive respiratory support only and inhaled nitric oxide therapy were found to be significantly associated with the risk of death. Among surviving infants born at < 32 weeks of gestation or with a birth weight < 1500 g, caffeine therapy and repeat mechanical ventilation were demonstrated to significantly associate with increased major morbidity risk. Conclusion Our study demonstrates the current clinical landscape of infants with NRF treated in the NICU, and, by proxy, highlights the ongoing advancements in the field of perinatal and neonatal intensive care in China.
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- 2024
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11. A nomogram to predict the risk of death during hospitalization in Chinese neonates with respiratory failure
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Bo Wang, Yue Wu, Jie Shao, Rui Cheng, Zuming Yang, Yan Xu, Guihua Shu, Hong Li, Huaiyan Wang, Jinlan Cai, Jinjun Zhou, Li Ye, Mengzhu Yu, Qin Zhou, Shuping Han, Songlin Liu, Xiaoqing Chen, Xinping Wu, Xiaoping Yin, Yan Gao, Zhidan Bao, Zhenguang Li, Zhaojun Pan, and Zhengying Li
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Death ,Neonate ,Prediction nomogram ,Respiratory failure ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Neonatal respiratory failure (NRF) is a critical condition with high morbidity and mortality rates. This study aimed to develop a nomogram prediction model to early predict the risk of death in Chinese neonates with NRF. Methods: A retrospective analysis was conducted on NRF neonates from 21 tertiary neonatal intensive care units (NICUs) across 13 prefecture-level cities in Jiangsu Province, China, from March 2019 to March 2022. NRF neonates from one random NICU were selected as the external validation set, while those from the remaining 20 NICUs were divided into the training set and the internal validation set at a 7:3 ratio. Death was the primary outcome. LASSO regression and multivariate logistic regression were used to identify the predictive factors from the training set and then the nomogram was constructed. Results: A total of 5387 neonates with NRF were included in the analysis. Among them, 3444 were in the training set, 1470 were in the internal validation set, and 473 were in the external validation set. The nomogram was constructed based on the eight predictors of the 1-min Apgar score, birth weight, gestational age, the relationship between birth weight and gestational age, mode of first respiratory support, inhaled nitric oxide, antenatal corticosteroids, and vasoactive drugs. The area under the curve of the nomogram in the training set, internal validation set, and external validation set was 0.763, 0.733, and 0.891, respectively. The P-values of the Hosmer-Lemeshow goodness of fit test were 0.638, 0.273, and 0.253, respectively. Brier scores were 0.066, 0.072, and 0.037, respectively. The decision curve analysis demonstrated a significant net benefit in all cases. These data indicate the good performance of the nomogram. Conclusions: This nomogram can serve as a reference for clinicians to identify high-risk neonates early and reduce the incidence of neonatal mortality.
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- 2024
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12. Comprehensive reliability assessment method for distribution networks considering IIDG low voltage ride-through control
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Shuai Zhang, Wenxia Liu, Haiyang Wan, Tianlong Wang, Rui Cheng, and Hanshen Li
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Distribution networks ,Reliability assessment ,Low voltage ride through ,Temporary fault ,Permanent fault ,Production of electric energy or power. Powerplants. Central stations ,TK1001-1841 - Abstract
Upon the large-scale integration of inverter-interfaced distributed generator (IIDG) into the grid, random faults in the distribution network can lead to momentary and sustained interruptions, significantly impacting system reliability. Although reliability methods have been widely used in distribution network adequacy assessment, using probabilistic method for reliability assessment including system dynamic security need to be investigated. To address this issue, a new method is designed to assess the reliability of distribution network using sequential Monte Carlo simulation. Firstly, the IIDG low-voltage ride-through (LVRT) control strategy is formulated, and the off-grid probability model for IIDG is developed based on the Gaussian distribution. Secondly, the component model is defined to consider the impact of random faults on power quality in security assessment. Following the fault tree analysis method, a protection action probability model was formulated to assess the failure probability of line current differential protection, IIDG anti-islanding protection, and reclosing protection. Finally, the method for momentary fault consequence analysis, based on depth-first search (DFS), and the method for sustained fault consequence analysis, based on the mixed-integer linear programming model, are developed. The study establishes a comprehensive probability reliability assessment framework. The validity of the method is demonstrated on the IEEE RBTS BUS6 F4 system, indicating good scalability.
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- 2024
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13. Neoadjuvant immunochemotherapy improves clinical outcomes of patients with esophageal cancer by mediating anti-tumor immunity of CD8+ T (Tc1) and CD16+ NK cells
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Yunlong He, Depeng Yang, Xiaoyu Lin, Jinfeng Zhang, Rui Cheng, Liangyu Cao, Lijun Yang, Mengmeng Zhang, Xinyue Shi, Xiyun Jin, Handi Sun, Haoxiu Sun, Jingyu Zang, Yu Li, Jianqun Ma, and Huan Nie
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esophageal cancer ,neoadjuvant immunochemotherapy ,tumor microenvironment ,CD8+ T cells ,CD16+ NK cells ,CD276 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundEsophageal cancer (ESCA) is one of the most common tumors in the world, and treatment using neoadjuvant therapy (NT) based on radiotherapy and/or chemotherapy has still unsatisfactory results. Neoadjuvant immunochemotherapy (NICT) has also become an effective treatment strategy nowadays. However, its impact on the tumor microenvironment (TME) and regulatory mechanisms on T cells and NK cells needs to be further elucidated.MethodsA total of 279 cases of ESCA who underwent surgery alone [non-neoadjuvant therapy (NONE)], neoadjuvant chemotherapy (NCT), and NICT were collected, and their therapeutic effect and survival period were compared. Further, RNA sequencing combined with biological information was used to analyze the expression of immune-related genes. Immunohistochemistry, immunofluorescence, and quantitative real-time PCR (qRT-PCR) were used to verify the activation and infiltration status of CD8+ T and CD16+ NK cells, as well as the function and regulatory pathway of killing tumor cells.ResultsPatients with ESCA in the NICT group showed better clinical response, median survival, and 2-year survival rates (p < 0.05) compared with the NCT group. Our RNA sequencing data revealed that NICT could promote the expression of immune-related genes. The infiltration and activation of immune cells centered with CD8+ T cells were significantly enhanced. CD8+ T cells activated by PD-1 inhibitors secreted more IFN-γ and cytotoxic effector factor cells through the transcription factor of EOMES and TBX21. At the same time, activated CD8+ T cells mediated the CD16+ NK cell activation and secreted more IFN-γ to kill ESCA cells. In addition, the immunofluorescence co-staining results showed that more CD276+ tumor cells and CD16+ NK cells were existed in pre-NCT and pre-NICT group. However, CD276+ tumor cells were reduced significantly in the post-NICT group, while they still appeared in the post-NCT group, which means that CD16+ NK cells can recognize and kill CD276+ tumor cells after immune checkpoint blocker (ICB) treatment.ConclusionNICT can improve the therapeutic effect and survival period of resectable ESCA patients. NICT could promote the expression of immune-related genes and activate CD8+ T and CD16+ NK cells to secrete more IFN-γ to kill ESCA cells. It provides a theoretical basis and clinical evidence for its potential as an NT strategy in ESCA.
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- 2024
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14. Tenascin-C modulates alveolarization in bronchopulmonary dysplasia
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Wei Liu, Yu Mao, Qianru Lv, Keyu Lu, Chunyu Yin, Rui Cheng, and Mingshun Zhang
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Bronchopulmonary dysplasia ,Extracellular matrix ,Tenascin-C ,Hyperoxia ,Epithelial cells ,ICAM-1 ,Pathology ,RB1-214 - Abstract
Abstract Bronchopulmonary dysplasia (BPD) is a chronic lung disease characterized by retarded alveolarization. Tenascin-C (TN-C), an extracellular matrix glycoprotein and soluble molecule, is involved in tissue morphogenesis. In the present study, we demonstrated that the level of TN-C in lung tissues was greater in a mouse model of BPD induced by 85% oxygen. TN-C deficiency, however, impaired alveolarization in the hyperoxia-induced BPD model. In contrast, a functional TN-C blocking antibody ameliorated alveolar dysplasia in BPD-like mice. Mechanistically, hyperoxia increased the soluble TN-C (sTN-C) released from respiratory epithelial cells. On one hand, low-dose sTN-C promoted lung epithelial cell proliferation and migration, which was mediated by ICAM-1. On the other hand, high-dose sTN-C hindered the proliferation and migration of epithelial cells. Overall, this study revealed that TN-C plays a dual role in lung alveolarization and that TN-C may be a target in BPD therapy.
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- 2024
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15. The role of nutrition in analysis of risk factors and short-term outcomes for late-onset necrotizing enterocolitis among very preterm infants: a nationwide, multicenter study in China
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Kun-Yao Hong, Yao Zhu, Fan Wu, Jian Mao, Ling Liu, Rong Zhang, Yan-Mei Chang, Wei Shen, Li-Xia Tang, Xiu-Zhen Ye, Yin-Ping Qiu, Li Ma, Rui Cheng, Hui Wu, Dong-Mei Chen, Ling Chen, Ping Xu, Hua Mei, San-Nan Wang, Fa-Lin Xu, Rong Ju, Zhi Zheng, Xiao-Mei Tong, Xin-Zhu Lin, and the Chinese Multicenter EUGR Collaborative Group
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Very preterm infants ,Late-onset NEC ,Risk factors, Breastfeeding ,Extrauterine growth restriction ,Late-onset sepsis ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Necrotizing enterocolitis (NEC) is a serious gastrointestinal disease, primarily affects preterm newborns and occurs after 7 days of life (late-onset NEC, LO-NEC). Unfortunately, over the past several decades, not much progress has been made in its treatment or prevention. This study aimed to analyze the risk factors for LO-NEC, and the impact of LO-NEC on short-term outcomes in very preterm infants (VPIs) with a focus on nutrition and different onset times. Method Clinical data of VPIs were retrospectively collected from 28 hospitals in seven different regions of China from September 2019 to December 2020. A total of 2509 enrolled VPIs were divided into 2 groups: the LO-NEC group and non-LO-NEC group. The LO-NEC group was divided into 2 subgroups based on the onset time: LO-NEC occurring between 8 ~ 14d group and LO-NEC occurring after 14d group. Clinical characteristics, nutritional status, and the short-term clinical outcomes were analyzed and compared among these groups. Results Compared with the non-LO-NEC group, the LO-NEC group had a higher proportion of anemia, blood transfusion, and invasive mechanical ventilation (IMV) treatments before NEC; the LO-NEC group infants had a longer fasting time, required longer duration to achieve the target total caloric intake (110 kcal/kg) and regain birthweight, and showed slower weight growth velocity; the cumulative dose of the medium-chain and long-chain triglyceride (MCT/LCT) emulsion intake in the first week after birth was higher and breastfeeding rate was lower. Additionally, similar results including a higher proportion of IMV, lower breastfeeding rate, more MCT/LCT emulsion intake, slower growth velocity were also found in the LO-NEC group occurring between 8 ~ 14d when compared to the LO-NEC group occurring after 14 d (all (P
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- 2024
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16. Feasibility of a clinical-radiomics combined model to predict the occurrence of stroke-associated pneumonia
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Haowen Luo, Jingyi Li, Yongsen Chen, Bin Wu, Jianmo Liu, Mengqi Han, Yifan Wu, Weijie Jia, Pengfei Yu, Rui Cheng, Xiaoman Wang, Jingyao Ke, Hongfei Xian, Jianglong Tu, and Yingping Yi
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Stroke-associated pneumonia ,Radiomics ,Prediction ,Acute ischemic stroke ,Magnetic resonance imaging ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Purpose To explore the predictive value of radiomics in predicting stroke-associated pneumonia (SAP) in acute ischemic stroke (AIS) patients and construct a prediction model based on clinical features and DWI-MRI radiomics features. Methods Univariate and multivariate logistic regression analyses were used to identify the independent clinical predictors for SAP. Pearson correlation analysis and the least absolute shrinkage and selection operator with ten-fold cross-validation were used to calculate the radiomics score for each feature and identify the predictive radiomics features for SAP. Multivariate logistic regression was used to combine the predictive radiomics features with the independent clinical predictors. The prediction performance of the SAP models was evaluated using receiver operating characteristics (ROC), calibration curves, decision curve analysis, and subgroup analyses. Results Triglycerides, the neutrophil-to-lymphocyte ratio, dysphagia, the National Institutes of Health Stroke Scale (NIHSS) score, and internal carotid artery stenosis were identified as clinically independent risk factors for SAP. The radiomics scores in patients with SAP were generally higher than in patients without SAP (P
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- 2024
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17. Complement activation in wasp venom-induced acute kidney injury
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Rui Cheng, Liang Xu, Jianhua Gong, Fanglin Yu, Ying Lv, Hai Yuan, and Fengqi Hu
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Acute kidney injury ,complement activation ,complement pathway ,inflammation ,wasp ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Previous studies have highlighted the significant role of complement activation in kidney injuries induced by rhabdomyolysis, intravascular hemolysis, sepsis, and ischemia-reperfusion. Nevertheless, the specific role and mechanism of complement activation in acute kidney injury (AKI) caused by wasp venom remain unclear. The aim of this study was to elucidate the specific complement pathway activated and investigate complement activation in AKI induced by wasp venom. In this study, a complement-depleted mouse model was used to investigate the role of complement in wasp venom-induced AKI. Mice were randomly categorized into control, cobra venom factor (CVF), AKI, and CVF + AKI groups. Compared to the AKI group, the CVF + AKI group showed improved pathological changes in kidneys and reduced blood urea nitrogen (BUN) levels. The expression levels of renal complement 3 (C3), complement 5 (C5), complement 1q (C1q), factor B (FB), mannose-binding lectin (MBL), and C5b-9 in AKI group were upregulated compared with the control group. Conversely, the renal tissue expression levels of C3, C5, C1q, FB, MBL, and C5b-9 were decreased in the CVF + AKI group compared to those in the AKI group. Complement activation occurs through all three pathways in AKI induced by wasp venom. Furthermore, complement depletion by CVF attenuates wasp venom-induced nephrotoxicity, suggesting that complement activation plays a primary role in the pathogenesis of wasp venom-induced AKI.
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- 2024
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18. HADH suppresses clear cell renal cell carcinoma progression through reduced NRF2-dependent glutathione synthesis
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Changbin Chu, Shangjing Liu, Zhiting He, Mingjun Wu, Jing xia, Hongxiang Zeng, Wenhua Xie, Rui Cheng, Xueya Zhao, and Xi Li
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Clear cell renal cell carcinoma ,HADH ,Non-target metabolomics ,Glutathione synthesis ,NRF2 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is a serious threat to human life. It is very important to clarify the pathogenesis of ccRCC. In this study we evaluated the clinical value of HADH and explored its role and mechanism in the malignant progression of ccRCC. Methods: HADH expression and its relationship with prognosis were analyzed using bioinformatics database. RT-PCR, Western blot and immunohistochemistry were used to examine the expression of HADH in ccRCC tissues and tissue microarrays. To examine the cell proliferation, apoptosis, migration and invasion ability, ccRCC cells with HADH overexpressed were constructed. Xenograft experiments were performed to determine the role of HADH. Non-target metabolomics was applied to explore the potential metabolic pathway by which HADH inhibited ccRCC progression. Plasmid pcDNA3.1-NRF2 was used to confirm whether HADH inhibited the process of ccRCC cells through NRF2-related glutathione (GSH) synthesis. Results: Bioinformatics database analysis showed that HADH expression was significantly decreased in ccRCC tissues, and its low expression predicted a poor prognosis. Both ccRCC tissues and tissue microarrays exhibited a significantly decreased HADH level compared with adjacent normal renal tissues. HADH overexpression inhibited the malignant behaviors of ccRCC cells. Furthermore, HADH overexpression attenuated GSH synthesis and induced oxidative stress damage. Exogenously increased NRF2 effectively attenuated the inhibitive effect of HADH overexpression on ccRCC cells. Conclusion: Our data revealed that HADH suppressed the malignant behaviors of ccRCC cells by attenuating GSH synthesis through inhibition of NRF2 nuclear translocation, and HADH might be a novel therapeutic target for ccRCC treatment.
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- 2024
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19. EZH2 Inhibition Enhances PD‐L1 Protein Stability Through USP22‐Mediated Deubiquitination in Colorectal Cancer
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Jiaqi Huang, Qianqian Yin, Yuqing Wang, Xin Zhou, Yunyun Guo, Yuanjun Tang, Rui Cheng, Xiaotong Yu, Jie Zhang, Chen Huang, Zhanya Huang, Jianlin Zhang, Zhengyang Guo, Xiao Huo, Yan Sun, Yanfang Li, Hao Wang, Jianling Yang, and Lixiang Xue
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colon cancer ,deubiquitinase ,epigenetics ,EZH2 ,PD‐L1 ,tumor immunity ,Science - Abstract
Abstract The regulation of PD‐L1 is the key question, which largely determines the outcome of the immune checkpoint inhibitors (ICIs) based therapy. However, besides the transcription level, the protein stability of PD‐L1 is closely correlated with its function and has drawn increasing attention. In this study, EZH2 inhibition enhances PD‐L1 expression and protein stability, and the deubiquitinase ubiquitin‐specific peptidase 22 (USP22) is identified as a key mediator in this process. EZH2 inhibition transcriptionally upregulates USP22 expression, and upregulated USP22 further stabilizes PD‐L1. Importantly, a combination of EZH2 inhibitors with anti‐PD‐1 immune checkpoint blockade therapy improves the tumor microenvironment, enhances sensitivity to immunotherapy, and exerts synergistic anticancer effects. In addition, knocking down USP22 can potentially enhance the therapeutic efficacy of EZH2 inhibitors on colon cancer. These findings unveil the novel role of EZH2 inhibitors in tumor immune evasion by upregulating PD‐L1, and this drawback can be compensated by combining ICI immunotherapy. Therefore, these findings provide valuable insights into the EZH2‐USP22‐PD‐L1 regulatory axis, shedding light on the optimization of combining both immune checkpoint blockade and EZH2 inhibitor‐based epigenetic therapies to achieve more efficacies and accuracy in cancer treatment.
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- 2024
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20. The associations of daytime napping and motoric cognitive risk syndrome: Findings from the China Health and Retirement Longitudinal Study
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Lixing Zhou, Yan Zhang, Meiling Ge, Gongchang Zhang, Rui Cheng, Yixin Liu, Xiaoli Chen, Xiaolei Liu, and Birong Dong
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MCR ,Nap ,Sleep duration ,Older adults ,CHARLS ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Introduction: Motoric cognitive risk syndrome (MCR), characterized by subjective cognitive complaints and slow gait in older populations, is associated with sleep duration. However, the association between MCR and daytime nap duration has not been thoroughly explored. Methods: Baseline data from the China Health and Retirement Longitudinal Study (CHARLS) were used in this study. MCR was defined as the coexistence of subjective cognitive complaints and objective slow gait speed without a history of dementia or mobility disability. Daytime nap duration was categorized into four groups: no napping, short napping (
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- 2024
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21. Beta diversity of grasshoppers and predatory beetles across steppes is closely associated with altitude and average annual precipitation in Ningxia, northwest China
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Haixiang Zhang, Ziyu Cao, Ying Wang, Xiaoyu Shi, Rui Cheng, Liping Ban, Rong Zhang, and Shuhua Wei
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Ningxia Steppe ,Grasshopper ,Predatory beetle ,Beta diversity ,Environmental factors ,Ecology ,QH540-549.5 - Abstract
Beta diversity analysis was conducted to identify factors affecting the beta diversity patterns of grasshoppers and predatory beetles in steppes and the primary environmental drivers of these patterns in Ningxia, northwest China. Data on the insects and environment of 42 steppe monitoring areas in Ningxia were analyzed. A total of 4335 insects of 50 species were captured from 42 monitoring areas, including 743 grasshoppers and 3592 predatory beetles belonging to 27 and 23 species, respectively. Non-metric multidimensional scale (NMDS) indicated a high similarity in the grasshopper communities between temperate meadow steppe (TMS) and temperate steppe (TS). Additionally, there was a high similarity among the predatory beetle communities between temperate desert steppe (TDS) and TS. Redundancy analysis (RDA) showed altitude (AL) and average annual precipitation (PP) could explain the interaction between insects and the environment to a large extent. Among the four steppes, the beta diversity of grasshoppers was predominantly driven by the richness component. In TMS and temperate steppe desertification (TSD), the beta diversity of predatory beetles was dominated by the richness component, whereas in TS and TDS, the beta diversity of predatory beetles was dominated by the turnover component. Mantel test indicated that a difference in the beta diversity of the grasshoppers and predatory beetles correlated positively with AL (P
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- 2024
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22. Enhancing neurosurgical navigation operation flow management through personal digital assistant technology: a prospective cohort study
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YaQin Quan, ManHong Zhang, HongMing Ji, and Rui Cheng
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personal digital assistant ,neurosurgery ,patient satisfaction ,nursing care ,digital health ,Public aspects of medicine ,RA1-1270 - Abstract
PurposeThis prospective cohort study aims to evaluate the impact of digital health technology especially Personal Digital Assistants (PDA) in neurosurgical procedure management, focusing on surgical safety check accuracy, efficiency, and patient satisfaction.MethodsThe study included 211 neurosurgical cases from January to December 2022. The control group of 106 patients followed traditional verification methods, while the experimental group of 105 patients used PDA. The PDA system facilitated real-time data collection, verification, and transmission. The study compared both groups in terms of check times, accuracy rates, and patient satisfaction, and used multivariate regression to assess the impact of baseline parameters on these outcomes.ResultsThe study found that the experimental group using the PDA system reduced the average verification time by approximately 8 min, achieving 100.0% accuracy in preoperative and postoperative checks, significantly better than the control group (91.5% pre- and post-operation). Multivariate regression confirmed a 48.1% reduction in postoperative verification time due to the PDA system (p
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- 2024
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23. Endoscopic submucosal dissection (ESD) for gastritis cystica profunda (GCP) with early gastric cancer: A propensity score matching analysis
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Wei Jiang, Liyi Bai, Shutian Zhang, and Rui Cheng
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Early gastric cancer ,Gastritis cystica profunda ,Endoscopic submucosal dissection ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background and aim: Cystic dilatation of the gastric glands within the mucosal layer is the hallmark of the rare condition known as gastritis cystica profunda (GCP). Although it has been proved that GCP is the precursor lesion for early gastric cancer (EGC), the management strategy of GCP-related EGC is not well established.The purpose of this research was to determine if ESD is effective and safe for GCP-related EGC. Methods: Patients with EGC who had ESD at Beijing Friendship Hospital between January 2015 and May 2023 were retrospectively included. All patients were divided into two groups: those with GCP-related EGC, and those with EGC alone. The two groups were matched 1:1 using the propensity score matching (PSM) method. Curative resection rate, postoperative adverse outcome rate (bleeding, perforation, stricture), and recurrence rate were the primary measures used to evaluate the efficacy and safety of ESD. Results: There were a total of 386 participants (44 with GCP and 342 with EGC alone). Following PSM, 44 patients were paired and analyzed separately. Except for the presence of cysts in EUS (multiple/single/none cyst: 12/2/5 versus 1/0/25, P
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- 2024
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24. A gene network database for the identification of key genes for diagnosis, prognosis, and treatment in sepsis
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Qingsheng Li, Lili Qu, Yurui Miao, Qian Li, Jing Zhang, Yongxue Zhao, and Rui Cheng
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Medicine ,Science - Abstract
Abstract Sepsis and sepsis-related diseases cause a high rate of mortality worldwide. The molecular and cellular mechanisms of sepsis are still unclear. We aim to identify key genes in sepsis and reveal potential disease mechanisms. Six sepsis-related blood transcriptome datasets were collected and analyzed by weighted gene co-expression network analysis (WGCNA). Functional annotation was performed in the gProfiler tool. DSigDB was used for drug signature enrichment analysis. The proportion of immune cells was estimated by the CIBERSORT tool. The relationships between modules, immune cells, and survival were identified by correlation analysis and survival analysis. A total of 37 stable co-expressed gene modules were identified. These modules were associated with the critical biology process in sepsis. Four modules can independently separate patients with long and short survival. Three modules can recurrently separate sepsis and normal patients with high accuracy. Some modules can separate bacterial pneumonia, influenza pneumonia, mixed bacterial and influenza A pneumonia, and non-infective systemic inflammatory response syndrome (SIRS). Drug signature analysis identified drugs associated with sepsis, such as testosterone, phytoestrogens, ibuprofen, urea, dichlorvos, potassium persulfate, and vitamin B12. Finally, a gene co-expression network database was constructed ( https://liqs.shinyapps.io/sepsis/ ). The recurrent modules in sepsis may facilitate disease diagnosis, prognosis, and treatment.
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- 2023
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25. Effect of different courses and durations of invasive mechanical ventilation on respiratory outcomes in very low birth weight infants
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Yang Yang, Xin-yue Gu, Zhen-lang Lin, Shu-lin Pan, Jian-hua Sun, Yun Cao, Shoo K. Lee, Jian-hui Wang, Rui Cheng, and the CHNN investigators
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Medicine ,Science - Abstract
Abstract This multicenter retrospective study was conducted to explore the effects of different courses and durations of invasive mechanical ventilation (MV) on the respiratory outcomes of very low birth weight infants (VLBWI) in China. The population for this study consisted of infants with birth weight less than 1500 g needing at least 1 course of invasive MV and admitted to the neonatal intensive care units affiliated with the Chinese Neonatal Network within 6 h of life from January 1st, 2019 to December 31st, 2020. Univariate and multivariate logistic regression analyses were performed to evaluate associations between invasive MV and respiratory outcomes. Adjusted odds ratios (ORs) were computed with the effects of potential confounders. (1) Among the 3183 VLBWs with a history of at least one course of invasive MV, 3155 (99.1%) met inclusion criteria and were assessed for the primary outcome. Most infants received one course (76.8%) and a shorter duration of invasive MV (62.16% with ventilation for 7 days or less). (2) In terms of the incidence of all bronchopulmonary dysplasia (BPD) (mild, moderate, and severe BPD), there were no significant differences between different invasive MV courses [For 2 courses, adjusted OR = 1.11 (0.88, 1.39); For 3 courses or more, adjusted OR = 1.07 (0.72, 1.60)]. But, with the duration of invasive MV prolonging, the OR of BPD increased [8–21 days, adjusted OR = 1.98 (1.59, 2.45); 22–35 days, adjusted OR = 4.37 (3.17, 6.03); ≥ 36 days, adjusted OR = 18.44 (10.98, 30.99)]. Concerning severe BPD, the OR increased not only with the course of invasive MV but also with the duration of invasive MV [For 2 courses, adjusted OR = 2.17 (1.07, 4.40); For 3 courses or more, adjusted OR = 2.59 (1.02, 6.61). 8–21 days, adjusted OR = 8.42 (3.22, 22.01); 22–35 days, adjusted OR = 27.82 (9.08, 85.22); ≥ 36 days, adjusted OR = 616.45 (195.79, > 999.999)]. (3) When the interaction effect between invasive MV duration and invasive MV course was considered, it was found that there were no interactive effects in BPD and severe BPD. Greater than or equal to three courses would increase the chance of severe BPD, death, and the requirement of home oxygen therapy. Compared with distinct courses of invasive MV, a longer duration of invasive MV (> 7 days) has a greater effect on the risk of BPD, severe BPD, death, and the requirement of home oxygen therapy.
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- 2023
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26. Prevotella copri alleviates sarcopenia via attenuating muscle mass loss and function decline
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Xiaolei Liu, Jiqiu Wu, Jingyi Tang, Zhigang Xu, Bailing Zhou, Yang Liu, Fengjuan Hu, Gongchang Zhang, Rui Cheng, Xin Xia, Yilong Chen, Hongyu Wu, Daoming Wang, Jirong Yue, Biao Dong, Jingyuan Fu, Haopeng Yu, and Birong Dong
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Brand chain amino acid ,Gut microbiome ,Prevotella copri ,Sarcopenia ,Serum metabolome ,Diseases of the musculoskeletal system ,RC925-935 ,Human anatomy ,QM1-695 - Abstract
Abstract Background The gut microbiome and fecal metabolites have been found to influence sarcopenia, but whether there are potential bacteria that can alleviate sarcopenia has been under‐investigated, and the molecular mechanism remains unclear. Methods To investigate the relationships between the gut microbiome, fecal metabolites and sarcopenia, subjects were selected from observational multi‐ethnic study conducted in Western China. Sarcopenia was diagnosed according to the criteria of the Asian Working Group for Sarcopenia 2014. The gut microbiome was profiled by shotgun metagenomic sequencing. Untargeted metabolomic analysis was performed to analyse the differences in fecal metabolites. We investigated bacterium with the greatest relative abundance difference between healthy individuals and sarcopenia patients, and the differences in metabolites associated with the bacteria, to verify its effects on muscle mass and function in a mouse model. Results The study included 283 participants (68.90% females, mean age: 66.66 years old) with and without sarcopenia (141 and 142 participants, respectively) and from the Han (98 participants), Zang (88 participants) and Qiang (97 participants) ethnic groups. This showed an overall reduction (15.03% vs. 20.77%, P = 0.01) of Prevotella copri between the sarcopenia and non‐sarcopenia subjects across the three ethnic groups. Functional characterization of the differential bacteria showed enrichment (odds ratio = 15.97, P = 0.0068) in branched chain amino acid (BCAA) metabolism in non‐sarcopenia group. A total of 13 BCAA and their derivatives have relatively low levels in sarcopenia. In the in vivo experiment, we found that the blood BCAA level was higher in the mice gavaged with live P. copri (LPC) (P
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- 2023
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27. Genetically predicted obstructive sleep apnea is causally associated with an increased risk for periodontitis
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Zhongqian Mi, Nan Meng, Yitao Zhang, Qianqian Wang, Shan Song, Rui Cheng, Xiaojiang Xu, Jinhua Gao, Feiyan Yu, and Xiuyun Ren
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Periodontitis ,Obstructive sleep apnea ,Causality ,Mendelian randomization ,Dentistry ,RK1-715 - Abstract
Abstract Background Although obstructive sleep apnea (OSA) and periodontitis are associated, whether this association is causative is uncertain. Methods We conducted a bidirectional Mendelian randomization (MR) analysis using data from publically accessible genome-wide association studies. The single-nucleotide polymorphisms (SNPs) for OSA were derived from 16,761 cases and 201,194 controls. The pooled data of periodontitis association involved up to 17,353 individuals. Disease-associated single-nucleotide polymorphisms were selected as an instrumental variable at the genome-wide significance level (p
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- 2023
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28. Functional requirement of alternative splicing in epithelial-mesenchymal transition of pancreatic circulating tumor
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Lixing Xiao, Haoxiu Sun, Rui Cheng, Rongrong Yang, Xiyun Jin, Zhaochun Xu, Yideng Cai, Yuexin Yang, Fenglan Pang, Guangfu Xue, Pingping Wang, Qinghua Jiang, and Huan Nie
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MT: Bioinformatics ,alternative splicing ,circulating tumor cell ,epithelial-to-mesenchymal transition ,pancreatic ductal adenocarcinoma ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Circulating tumor cells (CTCs) that undergo epithelial-to-mesenchymal transition (EMT) can provide valuable information regarding metastasis and potential therapies. However, current studies on the EMT overlook alternative splicing. Here, we used single-cell full-length transcriptome data and mRNA sequencing of CTCs to identify stage-specific alternative splicing of partial EMT and mesenchymal states during pancreatic cancer metastasis. We classified definitive tumor and normal epithelial cells via genetic aberrations and demonstrated dynamic changes in the epithelial-mesenchymal continuum in both epithelial cancer cells and CTCs. We provide the landscape of alternative splicing in CTCs at different stages of EMT, uncovering cell-type-specific splicing patterns and splicing events in cell surface proteins suitable for therapies. We show that MBNL1 governs cell fate through alternative splicing independently of changes in gene expression and affects the splicing pattern during EMT. We found a high frequency of events that contained multiple premature termination codons and were enriched with C and G nucleotides in close proximity, which influence the likelihood of stop codon readthrough and expand the range of potential therapeutic targets. Our study provides insights into the EMT transcriptome’s dynamic changes and identifies potential diagnostic and therapeutic targets in pancreatic cancer.
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- 2024
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29. Hyperglycemia in pregnancy did not worsen the short-term outcomes of very preterm infants: a propensity score matching study
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Ying Li, Wei Shen, Rong Zhang, Jian Mao, Ling Liu, Yan-Mei Chang, Xiu-Zhen Ye, Yin-Ping Qiu, Li Ma, Rui Cheng, Hui Wu, Dong-Mei Chen, Ling Chen, Ping Xu, Hua Mei, San-Nan Wang, Fa-Lin Xu, Rong Ju, Xiao-Mei Tong, Xin-Zhu Lin, and Fan Wu
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hyperglycemia in pregnancy ,preterm infant ,outcomes ,complication ,growth retardation ,Pediatrics ,RJ1-570 - Abstract
BackgroundHyperglycemia in pregnancy (HGP) has generally been considered a risk factor associated with adverse outcomes in offspring, but its impact on the short-term outcomes of very preterm infants remains unclear.MethodsA secondary analysis was performed based on clinical data collected prospectively from 28 hospitals in seven regions of China from September 2019 to December 2020. According to maternal HGP, all infants were divided into the HGP group or the non-HGP group. A propensity score matching analysis was used to adjust for confounding factors, including gestational age, twin or multiple births, sex, antenatal steroid administration, delivery mode and hypertensive disorders of pregnancy. The main complications and the short-term growth status during hospitalization were evaluated in the HGP and non-HGP groups.ResultsA total of 2,514 infants were eligible for analysis. After matching, there were 437 infants in the HGP group and 874 infants in the non-HGP group. There was no significant difference between the two groups in main complications including respiratory distress syndrome, bronchopulmonary dysplasia, necrotizing enterocolitis, retinopathy of prematurity, patent ductus arteriosus, culture positive sepsis, intraventricular hemorrhage, periventricular leukomalacia, anemia, feeding intolerance, metabolic bone disease of prematurity, or parenteral nutrition-associated cholestasis. The incidences of extrauterine growth retardation and increased growth retardation for weight and head circumference in the non-HGP group were all higher than those in the HGP group after matching (P
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- 2024
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30. Kelp as a biomonitor of persistent organic pollutants in coastal areas of China: Contamination levels and human health risk
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Rui Cheng, Jing Sun, Zhi-tong Liu, Wei Wu, Min Song, Yu-ting Lu, and Tai-jun Hang
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PCBs ,PBDEs ,EBFRs ,Kelp ,Risk assessment ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Kelp, the brown alga distributed in coastal areas all over the world, is also an important medicine food homology product in China. However, the levels and profiles of persistent organic pollutants (POPs) in kelp have not been thoroughly investigated to date. Polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and emerging bromine flame retardants (eBFRs) were evaluated in 41 kelp samples from the main kelp producing areas in China. The concentrations of total PCBs, PBDEs and eBFRs were in the range of 0.321–4.24 ng/g dry weight (dw), 0.255–25.5 ng/g dw and 3.00 × 10−3-47.2 ng/g dw in kelp, respectively. The pollutant pattern was dominated by decabromodiphenyl ethane (DBDPE, 13.0 ± 11.7 ng/g dw) followed in decreasing order by BDE-209 (2.74 ± 4.09 ng/g dw), CB-11 (1.32 ± 1.06 ng/g dw). The tested results showed that kelp could reflect the pollution status of PCBs, PBDEs and eBFRs, indicating the suitability of kelp as a biomonitor of these harmful substances. Finally, the data obtained was used to evaluate human non-cancer and cancer risks of PCBs and PBDEs via kelp consumption for Chinese. Though the calculated risk indices were considered acceptable according to the international standards even in the worst scenarios, the POPs levels in kelp should be monitored continuously as a good environmental indicator.
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- 2024
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31. Regulation of EZH2 protein stability: new mechanisms, roles in tumorigenesis, and roads to the clinic
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Yunyun Guo, Rui Cheng, Yuqing Wang, Maria E. Gonzalez, Hongshan Zhang, Yang Liu, Celina G. Kleer, and Lixiang Xue
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EZH2 ,Post-translational modification ,Protein stability ,Targeted cancer therapy ,Medicine ,Medicine (General) ,R5-920 - Abstract
Summary: The importance of EZH2 as a key methyltransferase has been well documented theoretically. Practically, the first EZH2 inhibitor Tazemetostat (EPZ6438), was approved by FDA in 2020 and is used in clinic. However, for most solid tumors it is not as effective as desired and the scope of clinical indications is limited, suggesting that targeting its enzymatic activity may not be sufficient. Recent technologies focusing on the degradation of EZH2 protein have drawn attention due to their potential robust effects. This review focuses on the molecular mechanisms that regulate EZH2 protein stability via post-translational modifications (PTMs), mainly including ubiquitination, phosphorylation, and acetylation. In addition, we discuss recent advancements of multiple proteolysis targeting chimeras (PROTACs) strategies and the latest degraders that can downregulate EZH2 protein. We aim to highlight future directions to expand the application of novel EZH2 inhibitors by targeting both EZH2 enzymatic activity and protein stability.
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- 2024
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32. Treatment of patent ductus arteriosus and short-term outcomes among extremely preterm infants: a multicentre cohort studyResearch in context
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Aimin Qian, Siyuan Jiang, Xinyue Gu, Shujuan Li, Xiaoping Lei, Wei Shi, Jianguo Zhou, Liyuan Hu, Tiantian Xiao, Yanping Zhu, Yun Cao, Lizhong Du, Wenhao Zhou, Shoo K. Lee, Rui Cheng, and Rong Yin
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Patent ductus arteriosus ,Extremely preterm infants ,Preterm infants ,Treatment ,Medicine (General) ,R5-920 - Abstract
Summary: Background: The optimal treatment strategy for patent ductus arteriosus (PDA) in extremely preterm infants is currently highly controversial. This study aimed to evaluate the association between PDA treatment and short-term outcomes among extremely preterm infants. Methods: This cohort study included all extremely preterm infants (≤27 and 6/7 weeks) who were admitted to hospitals participating in the Chinese Neonatal Network from January 2019 to December 2021, and were diagnosed to have PDA by echocardiogram. PDA treatment was defined as medical treatment and/or surgical ligation of PDA during hospitalization. Short-term outcomes included death, bronchopulmonary dysplasia (BPD), death/BPD, retinopathy of prematurity, necrotizing enterocolitis, and severe brain injury. Multivariate logistic regression was used to evaluate the association between PDA treatment and outcomes. Subgroup analysis were performed among infants with different respiratory support on 3 and 7 days of life. Findings: A total of 2494 extremely preterm infants with the diagnosis of PDA were enrolled, of which 1299 (52.1%) received PDA treatment. PDA treatment was significantly associated with lower risk of death (adjusted odds ratio, 0.48; 95% confidence interval, 0.38–0.60). The decreased risk of death was accompanied by increased risk of BPD and death/BPD. In subgroup analysis according to respiratory support, PDA treatment was associated with lower risk of death among infants who required invasive ventilation. However, the beneficial effect on death was not significant among infants who did not require invasive ventilation. Interpretation: PDA treatment was associated with reduced mortality in extremely preterm infants, but this beneficial effect was mainly present among infants who required invasive ventilation. Funding: This study was funded by the Shanghai Science and Technology Commission's Scientific and Technological Innovation Action Plan (21Y21900800) and the Canadian Institutes of Health Research (CTP87518).
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- 2024
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33. An integrated single-shot spectrometer with large bandwidth-resolution ratio and wide operation temperature range
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Ang Li, Chang Wang, Feixia Bao, Wenji Fang, Yuxin Liang, Rui Cheng, and Shilong Pan
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Optical spectrometers ,Silicon photonics ,Computational spectrometers ,Photonic integrated circuits ,Applied optics. Photonics ,TA1501-1820 - Abstract
Abstract There has been a rapidly growing demand for low-cost, integrated single-shot spectrometers to be embedded in portable intelligent devices. Even though significant progress has been made in this area, two major problems are still remaining, namely the high temperature sensitivity and poor bandwidth-resolution ratio (BRR) that can’t meet the requirement of most applications. In this work, we present an integrated single-shot spectrometer relying on a silicon photonic circuit that has a footprint less than 3mm2, but could achieve broad operation bandwidth about 100 nm and high resolution up to 0.1 nm (with a BRR ~ 1000). Moreover, for the first time, we demonstrate an integrated spectrometer that could operate within a wide temperature range (between 10 and 70 degrees Celsius) without additional power consumption for temperature management.
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- 2023
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34. Post-translational modification of CDK1–STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties
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Xiaodong Xu, Yimin Ding, Junbin Jin, Chengjie Xu, Wenyi Hu, Songtao Wu, Guoping Ding, Rui Cheng, Liping Cao, and Shengnan Jia
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CDK1 ,Fisetin ,Cancer stem cells ,STAT3 ,Pancreatic ductal adenocarcinoma ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Pancreatic cancer stem cells (CSCs) promote pancreatic ductal adenocarcinoma (PDAC) tumorigenesis and chemoresistance. Cyclin-dependent kinase 1 (CDK1) plays an important role in tumor initiation in other tumors, but the function of CDK1 in PDAC remains unclear. Fisetin is a bioactive flavonoid with anti-tumor properties in multiple tumors, while its function in CSCs remains elusive. Results In this study, we demonstrated that CDK1 was correlated with prognosis and was highly expressed in pancreatic cancer tissue and gemcitabine-resistant cells. Silencing CDK1 impaired tumor stemness and reduced a subset of CSCs. We found that fisetin blocked the kinase pocket domain of CDK1 and inhibited pancreatic CSC characteristics. Using acetylation proteomics analysis and phosphorylation array assay, we confirmed that fisetin reduced CDK1 expression and increased CDK1 acetylation at lysine 33 (K33), which resulted in the suppression of CDK1 phosphorylation. Silencing CDK1 or STAT3 suppressed tumor stemness properties, while overexpressing CDK1 or STAT3 showed the opposite effect. Mutation or acetylation of CDK1 at K33 weakened STAT3 phosphorylation at Y705, impairing the expression of stem-related genes and pancreatic cancer stemness. In addition, lack of histone deacetylase 3 (HDAC3), which deacetylates CDK1, contributed to weakening STAT3 phosphorylation by regulating the post-translational modification of CDK1, thereby decreasing the stemness of PDAC. Moreover, our results revealed that fisetin enhanced the effect of gemcitabine through eliminating a subpopulation of pancreatic CSCs by inhibiting the CDK1–STAT3 axis in vitro and in vivo. Conclusion Our findings highlight the role of post-translational modifications of CDK1–STAT3 signaling in maintaining cancer stemness of PDAC, and indicated that targeting the CDK1–STAT3 axis with inhibitors such as fisetin is a potential therapeutic strategy to diminish drug resistance and eliminate PDAC.
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- 2023
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35. Real-world evidence regarding the growth of very premature infants with small for gestational age after birth: a multicenter survey in China
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Xue-Rong Huang, Wei Shen, Fan Wu, Jian Mao, Ling Liu, Yan-Mei Chang, Rong Zhang, Xiu-Zhen Ye, Yin-Ping Qiu, Li Ma, Rui Cheng, Hui Wu, Dong-Mei Chen, Ling Chen, Ping Xu, Hua Mei, San-Nan Wang, Fa-Lin Xu, Rong Ju, Zhi Zheng, Xin-Zhu Lin, Xiao-Mei Tong, and the Chinese Multicenter EUGR Collaborative Group
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Extrauterine growth retardation ,Extremely premature infants ,GV ,Nutrition ,Small for gestational age ,Z score ,Pediatrics ,RJ1-570 - Abstract
Abstract Background To analyze the real-world growth pattern of very premature infants (VPI) with small for gestational age (SGA) after birth by using the ΔZ value of weight at discharge. Methods The clinical data were collected from 28 hospitals in China from September 2019 to December 2020. They were divided into the EUGR(Extrauterine Growth Restriction) and the non-EUGR group according to the criterion of ΔZ value of weight at discharge
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- 2023
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36. Does Early Endoscopy Affect the Clinical Outcomes of Patients with Acute Nonvariceal Upper Gastrointestinal Bleeding? A Systematic Review and Meta-Analysis
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Liyi Bai, Wei Jiang, Rui Cheng, Yan Dang, Li Min, and Shutian Zhang
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endoscopy ,gastrointestinal hemorrhage ,severity of illness index ,mortality ,meta-analysis ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background/Aims: In patients with acute nonvariceal upper gastrointestinal bleeding (ANVUGIB), the optimal timing of endoscopy is still a matter of dispute. We conducted a systematic review and meta-analysis to determine the clinical benefit of early endoscopy. Methods: A literature search of the MEDLINE, Embase, and Cochrane databases was conducted to identify publications from inception to March 1, 2022. Eligible studies included observational cohort studies and randomized controlled trials that reported clinical outcomes of endoscopy in patients with ANVUGIB. ANVUGIB patients who underwent endoscopy within 24 hours of admission were considered to have had an early endoscopy. The primary outcome was the mortality rate in ANVUGIB patients who had early or nonearly endoscopy. Results: The final analysis included five randomized controlled studies (RCTs) and 20 observational studies from the 1,206 identified articles. The mortality rate was not significantly reduced among patients who received endoscopy performed within 24 hours, whether in cohort studies nor in RCTs. For subgroup analysis, a higher mortality rate was found only among patients who received very early endoscopy within 12 hours (odds ratio, 1.66; p
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- 2023
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37. Review of the Idaea proximaria complex (Lepidoptera, Geometridae, Sterrhinae) with descriptions of four new species
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Rui Cheng, Da-Yong Xue, Nan Jiang, and Hong-Xiang Han
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Zoology ,QL1-991 - Abstract
The Idaea proximaria complex is reviewed and four new species are described from China: Idaea rectangularis Cheng & Han, sp. nov. from Guangxi and Fujian provinces, Idaea rectispina Cheng & Han, sp. nov. from Hunan province, Idaea setosa Xue & Han, sp. nov. from Hainan province, and Idaea linearis Xue & Han, sp. nov. from Hubei and Shaanxi provinces. Illustrations of adults and genitalia of the new species and known species are presented, and the variations in the form of the aedeagus of Idaea proximaria are discussed.
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- 2023
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38. Intubation at Birth Is Associated with Death after Pulmonary Hemorrhage in Very Low Birth Weight Infants
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Yong-Ping Sun, Hou-Bing Qin, Yun Feng, Yun-Su Zou, Yun Liu, Rui Cheng, and Yang Yang
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pulmonary hemorrhage ,postnatal tracheal intubation ,preterm infants ,mechanical ventilation ,risk factor ,Pediatrics ,RJ1-570 - Abstract
Objective: This retrospective cohort study was performed to clarify the association between intubation in the delivery room and the mortality after pulmonary hemorrhage in very low birth weight infants (VLBWIs) during hospitalization. Methods: The study participants were screened from the VLBWIs admitted to the neonatal intensive care unit (NICU) of the Children’s Hospital Affiliated to Nanjing Medical University from 31 July 2019 to 31 July 2022. The newborns who ultimately were included were those infants who survived until pulmonary hemorrhage was diagnosed. These subjects were divided into the intubation-at-birth group (n = 29) and the non-intubation-at-birth group (n = 35), retrospectively. Results: Univariate analysis found that the intubation group had a higher mortality and shorter hospital stay than the non-intubation group (p < 0.05) (for mortality: 25/29 (86.21%) in intubation group versus 14/35 (40.00%) in non-intubation group). By multivariate analysis, the result further showed that intubation in the delivery room was related to shorter survival time and higher risk of death (adjusted hazard ratio: 2.341, 95% confidence interval: 1.094–5.009). Conclusions: Intubation at birth suggested a higher mortality in the VLBWIs when pulmonary hemorrhage occurred in the NICU.
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- 2024
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39. Identification of Growth-Promoting Bacterial Resources by Investigating the Microbial Community Composition of Polyporus umbellatus Sclerotia
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Tianrui Liu, Rui Cheng, Zhongyi Hua, Haiyun Gao, Chu Wang, Hui Li, and Yuan Yuan
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high-throughput sequencing ,sustainable cultivation ,Polyporus umbellatus polysaccharide ,combined bacterial liquid ,bioactive microbial resources ,Biology (General) ,QH301-705.5 - Abstract
The sclerotium of the edible mushroom Polyporus umbellatus (Zhuling) exhibits various medicinal properties. However, given its long growth cycle and overexploitation, wild resources are facing depletion. Macrofungal growth depends on diverse microbial communities; however, the impact of soil bacteria on P. umbellatus development is unknown. Here, we combined high-throughput sequencing and pure culturing to characterize the diversity and potential function of bacteria and fungi inhabiting the P. umbellatus sclerotium and tested the bioactivities of their isolates. Fungal operational taxonomic units (OTUs) were clustered and classified, revealing 1275 genera. Bacterial OTUs yielded 891 genera. Additionally, 81 bacterial and 15 fungal strains were isolated from P. umbellatus sclerotia. Antagonism assays revealed three bacterial strains (FN2, FL19, and CL15) promoting mycelial growth by producing indole-3-acetic acid, solubilizing phosphate, and producing siderophores, suggesting their role in regulating growth, development, and production of active compounds in P. umbellatus. FN2-CL15 combined with bacterial liquid promoted growth and increased the polysaccharide content of P. umbellatus mycelia. This study reports new bioactive microbial resources for fertilizers or pesticides to enhance the growth and polysaccharide accumulation of P. umbellatus mycelia and offers guidance for exploring the correlation between medicinal macrofungi and associated microbial communities.
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- 2024
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40. Motor Behavior Regulation of Rat Robots Using Integrated Electrodes Stimulated by Micro-Nervous System
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Jiabing Huo, Le Zhang, Xiangyu Luo, Yongkang Rao, Peili Cao, Xiaojuan Hou, Jian He, Jiliang Mu, Wenping Geng, Haoran Cui, Rui Cheng, and Xiujian Chou
- Subjects
micro-nervous system ,integrated electrodes ,rat robots ,motor behavior regulation ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
As a cutting-edge technology, animal robots based on living organisms are being extensively studied, with potential for diverse applications in the fields of neuroscience, national security, and civil rescue. However, it remains a significant challenge to reliably control the animal robots with the objective of protecting their long-term survival, and this has seriously hindered their practical implementation. To address this issue, this work explored the use of a bio-friendly neurostimulation system that includes integrated stimulation electrodes together with a remote wireless stimulation circuit to control the moving behavior of rat robots. The integrated electrodes were implanted simultaneously in four stimulation sites, including the medial forebrain bundle (MFB) and primary somatosensory cortex, barrel field (S1BF). The control system was able to provide flexibility in adjusting the following four stimulation parameters: waveform, amplitude, frequency, and duration time. The optimized parameters facilitated the successful control of the rat’s locomotion, including forward movement and left and right turns. After training for a few cycles, the rat robots could be guided along a designated route to complete the given mission in a maze. Moreover, it was found that the rat robots could survive for more than 20 days with the control system implanted. These findings will ensure the sustained and reliable operation of the rat robots, laying a robust foundation for advances in animal robot regulation technology.
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- 2024
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41. Caspase-1 Deficiency Modulates Adipogenesis through Atg7-Mediated Autophagy: An Inflammatory-Independent Mechanism
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Yumeng Wang, Gaojun Chen, Min Xu, Yewei Cui, Weijiong He, Hongxiang Zeng, Ting Zeng, Rui Cheng, and Xi Li
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Caspase-1 ,adipogenesis ,autophagy ,Atg7 ,Microbiology ,QR1-502 - Abstract
Obesity stands as a significant risk factor for type 2 diabetes, hyperlipidemia, and cardiovascular diseases, intertwining increased inflammation and decreased adipogenesis with metabolic disorders. Studies have highlighted the correlation between Caspase-1 and inflammation in obesity, elucidating its essential role in the biological functions of adipose tissue. However, the impact of Caspase-1 on adipogenesis and the underlying mechanisms remain largely elusive. In our study, we observed a positive correlation between Caspase-1 expression and obesity and its association with adipogenesis. In vivo experiments revealed that, under normal diet conditions, Caspase-1 deficiency improved glucose homeostasis, stimulated subcutaneous adipose tissue expansion, and enhanced adipogenesis. Furthermore, our findings indicate that Caspase-1 deficiency promotes the expression of autophagy-related proteins and inhibits autophagy with 3-MA or CQ blocked Caspase-1 deficiency-induced adipogenesis in vitro. Notably, Caspase-1 deficiency promotes adipogenesis via Atg7-mediated autophagy activation. In addition, Caspase-1 deficiency resisted against high-fat diet-induced obesity and glucose intolerance. Our study proposes the downregulation of Caspase-1 as a promising strategy for mitigating obesity and its associated metabolic disorders.
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- 2024
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42. Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
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Binghua Xu, Chaoyang Zhang, Yan Gu, Rui Cheng, Dayue Huang, Xin Liu, and Yudong Sun
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Medicine ,Science - Abstract
Abstract Leaf color mutants are important materials for studying chloroplast and photomorphogenesis, and can function as basic germplasms for genetic breeding. In an ethylmethanesulfonate mutagenesis population of watermelon cultivar “703”, a chlorophyll-deficient mutant with yellow leaf (Yl2) color was identified. The contents of chlorophyll a, chlorophyll b, and carotenoids in Yl2 leaves were lower than those in wild-type (WT) leaves. The chloroplast ultrastructure in the leaves revealed that the chloroplasts in Yl2 were degraded. The numbers of chloroplasts and thylakoids in the Yl2 mutant were lower, resulting in lower photosynthetic parameters. Transcriptomic analysis identified 1292 differentially expressed genes, including1002 upregulated and 290 downregulated genes. The genes involved in chlorophyll biosynthesis (HEMA, HEMD, CHL1, CHLM, and CAO) were significantly downregulated in the Yl2 mutant, which may explain why chlorophyll pigment content was lower than that in the WT. Chlorophyll metabolism genes such as PDS, ZDS and VDE, were upregulated, which form the xanthophyll cycle and may protect the yellow‒leaves plants from photodamage. Taken together, our findings provide insight into the molecular mechanisms of leading to leaf color formation and chloroplast development in watermelon.
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- 2023
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43. MG1 interacts with a protease inhibitor and confers resistance to rice root-knot nematode
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Xiaomin Wang, Rui Cheng, Daochao Xu, Renliang Huang, Haoxing Li, Liang Jin, Yufeng Wu, Jiuyou Tang, Changhui Sun, Deliang Peng, Chengcai Chu, and Xiaoli Guo
- Subjects
Science - Abstract
Abstract The rice root-knot nematode (Meloidogyne graminicola) is one of the most destructive pests threatening rice (Oryza sativa L.) production in Asia; however, no rice resistance genes have been cloned. Here, we demonstrate that M. GRAMINICOLA-RESISTANCE GENE 1 (MG1), an R gene highly expressed at the site of nematode invasion, determines resistance against the nematode in several rice varieties. Introgressing MG1 into susceptible varieties increases resistance comparable to resistant varieties, for which the leucine-rich repeat domain is critical for recognizing root-knot nematode invasion. We also report transcriptome and cytological changes that are correlated with a rapid and robust response during the incompatible interaction that occurs in resistant rice upon nematode invasion. Furthermore, we identified a putative protease inhibitor that directly interacts with MG1 during MG1-mediated resistance. Our findings provide insight into the molecular basis of nematode resistance as well as valuable resources for developing rice varieties with improved nematode resistance.
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- 2023
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44. Identification of shared characteristics in tumor-infiltrating T cells across 15 cancers
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Xiyun Jin, Yideng Cai, Guangfu Xue, Jinhao Que, Rui Cheng, Yuexin Yang, Lixing Xiao, Xiaoyu Lin, Chang Xu, Pingping Wang, Zhaochun Xu, Huan Nie, and Qinghua Jiang
- Subjects
MT: Bioinformatics ,tumor-infiltrating T cells ,pan-cancer ,single-cell sequencing ,TF regulon ,state transition ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Tumor-infiltrating T cells are essential players in tumor immunotherapy. Great progress has been achieved in the investigation of T cell heterogeneity. However, little is well known about the shared characteristics of tumor-infiltrating T cells across cancers. In this study, we conduct a pan-cancer analysis of 349,799 T cells across 15 cancers. The results show that the same T cell types had similar expression patterns regulated by specific transcription factor (TF) regulons across cancers. Multiple T cell type transition paths were consistent in cancers. We found that TF regulons associated with CD8+ T cells transitioned to terminally differentiated effector memory (Temra) or exhausted (Tex) states were associated with patient clinical classification. We also observed universal activated cell-cell interaction pathways of tumor-infiltrating T cells in all cancers, some of which specifically mediated crosstalk in certain cell types. Moreover, consistent characteristics of TCRs in the aspect of variable and joining region genes were found across cancers. Overall, our study reveals common features of tumor-infiltrating T cells in different cancers and suggests future avenues for rational, targeted immunotherapies.
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- 2023
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45. A dynamic multi-attribute group decision-making method with R-numbers based on MEREC and CoCoSo method
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Rui Cheng, Jianping Fan, and Meiqin Wu
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R-numbers ,Einstein operator ,MEREC ,CoCoSo method ,Dynamic multi-attribute group decision-making ,Electronic computers. Computer science ,QA75.5-76.95 ,Information technology ,T58.5-58.64 - Abstract
Abstract Dynamic multi-attribute group decision-making (DMAGDM) is a widespread practice in which evaluations are provided by multiple decision-makers at various times and early evaluations impact later evaluations. Additionally, attributes and alternatives can be added or removed over time. An R-numbers DMAGDM method is developed based on the advantages of R-numbers in capturing risks. This paper introduces the R-numbers Einstein weighted averaging (RNEWA) operator and R-numbers weighted Einstein geometric (RNEWG) operator, which are distinct from conventional algebraic operations, and examines their properties. Moreover, an expert weight determination model is constructed using the similarity measure of R-numbers. The attribute weight determination model in the R-numbers environment is also proposed with the method based on the criteria removal effects method (MEREC). A static rating calculation model, which utilizes the combination compromise solution (CoCoSo) method in the R-numbers environment, is built using the RNEWA operator and RNEWG operator. Furthermore, a new dynamic rating calculation model is proposed which does not require storage of all decision information over time. Finally, the applicability and effectiveness of the R-numbers DMAGDM method is demonstrated through a case study on supply chain risk assessment of manufacturing enterprises.
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- 2023
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46. An efficient and provably secure certificateless protocol for the power internet of things
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Kehe Wu, Jiyu Zhang, Xiaochen Jiang, Rui Cheng, Xiaoliang Zhang, and Jie Tong
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Power IoT ,Certificateless authentication ,eCK security model ,Anonymity ,Provable security ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The Power Internet of Things (IoT) is a specific application of the IoT in the power grid. The threat existing in the Power IoT mainly comes from the terminal deployed in the open environment, which has the risk of being tampered with and impersonated. Thus, we design a certificateless anonymous key agreement protocol for the Power IoT to solve the problem of terminal access. The proposed protocol completes session key agreement in only one round exchange of key materials on the basis of certificatelessness, which saves the cost of certificate management. It also achieves anonymity by encrypting the authentication information to ensure the identity privacy of terminals. In addition, the protocol prevents malicious connections of internal systems by setting up software defined perimeter (SDP) controllers to complete pre-access authentication of terminals. Moreover, an improved eCK security model is designed and applied to demonstrate the proposed protocol. An experiment and analyses indicate that our scheme has advantages in terms of the communication and computation costs that are suitable for Power IoT.
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- 2023
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47. Surgical and medical co-management optimizes surgical outcomes in older patients with chronic diseases undergoing robot-assisted laparoscopic radical prostatectomy
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Wenning Lu, Chaoyang Liu, Jing He, Rong Wang, Dewei Gao, and Rui Cheng
- Subjects
Robot-assisted laparoscopic radical prostatectomy ,surgical and medical co-management ,older ,complications ,preoperative ,postoperative ,Medicine (General) ,R5-920 ,Physiology ,QP1-981 - Abstract
AbstractIntroduction While robotic-assisted laparoscopic radical prostatectomy (RRP) is a standard mode for localized prostate cancer (PC), the risk of complications in older patients with chronic diseases and complex medical conditions can be a deterrent to surgery. Surgical and medical co-management (SMC) is a new strategy to improve patients’ healthcare outcomes in surgical settings.Methods We reviewed the clinical data of older patients with chronic diseases who were cared for with SMC undergoing RRP in our hospital in the past 3 years and compared them with the clinical data from the general urology ward. Preoperative conditions and related indicators of recovery, and incidence of postoperative complications with the Clavien Grade System were compared between these two groups.Results The indicators of recovery were significantly better, and the incidence rates of complications were significantly reduced in the SMC group at grades I–IV (p
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- 2023
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48. Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
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Qikai Yin, Rui Cheng, Xiuyan Xu, Ziqian Xu, Jing Wang, Shihong Fu, Hongbin Xu, Shaozai Zhang, Ying He, Fan Li, Songtao Xu, Xiaoqing Lu, Huanyu Wang, Bin Wang, and Guodong Liang
- Subjects
lichuan virus (LICV) ,Orthobunyavirus ,Tete virus ,Culicoides biting midges ,evolution ,Microbiology ,QR1-502 - Abstract
In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 cells, and the virus titer was 1×105.6 pfu/mL. After inoculation with the virus, suckling mice developed disease and died. The nucleotide and amino sequence analysis showed that the JXLC1806-2 virus genome was composed of S, M and L segments. Phylogenetic analysis showed that the S, M and L genes of JXLC1806-2 virus belonged to the Tete serogroup, Orthobunyavirus, but formed an independent evolutionary branch from the other members of the Tete serogroup. The results showed that the JXLC1806-2 virus, which was named as Lichuan virus, is a new member of Tete serogroup, and this is the first time that a Tete serogroup virus has been isolated in China.
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- 2023
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49. Dynamic Mosaic algorithm for data augmentation
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Yuhua Li, Rui Cheng, Chunyu Zhang, Ming Chen, Hui Liang, and Zicheng Wang
- Subjects
data augmentation ,mosaic algorithm ,yolov5 ,deep learning ,object detection ,Biotechnology ,TP248.13-248.65 ,Mathematics ,QA1-939 - Abstract
Convolutional Neural Networks (CNNs) have achieved remarkable results in the computer vision field. However, the newly proposed network architecture has deeper network layers and more parameters, which is more prone to overfitting, resulting in reduced recognition accuracy of the CNNs. To improve the recognition accuracy of the model of image recognition used in CNNs and overcome the problem of overfitting, this paper proposes an improved data augmentation approach based on mosaic algorithm, named Dynamic Mosaic algorithm, to solve the problem of the information waste caused by the gray background in mosaic images. This algorithm improves the original mosaic algorithm by adding a dynamic adjustment step that reduces the proportion of gray background in the mosaic image by dynamically increasing the number of spliced images. Moreover, to relieve the problem of network overfitting, also a Multi-Type Data Augmentation (MTDA) strategy, based on the Dynamic Mosaic algorithm, is introduced. The strategy divides the training samples into four parts, and each part uses different data augmentation operations to improve the information variance between the training samples, thereby preventing the network from overfitting. To evaluate the effectiveness of the Dynamic Mosaic algorithm and the MTDA strategy, we conducted a series of experiments on the Pascal VOC dataset and compared it with other state-of-the-art algorithms. The experimental results show that the Dynamic Mosaic algorithm and MTDA strategy can effectively improve the recognition accuracy of the model, and the recognition accuracy is better than other advanced algorithms.
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- 2023
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50. The effect of APN, hs-CRP and APN/hs-CRP in periodontitis with DAA
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Rui Cheng, Xiaojiang Xu, Shurong Yang, Zhongqian mi, Yong Zhao, Chong Wang, Xuexue Shi, Jinhua Gao, Feiyan Yu, and Xiuyun Ren
- Subjects
Chronic periodontitis ,DAA ,APN ,Hs-CRP ,Lipid ,Dentistry ,RK1-715 - Abstract
Abstract Background Common chronic infections induced low-grade inflammation has been correlated with atherosclerosis as supported by strong evidence. The balance between pro-and anti-inflammatory factors was exploited to elucidate the effects of chronic periodontitis on diabetes-associated atherosclerosis. Methods Study subjects encompassed 30 SPF male rats randomly divided into four groups: A group (NC), B group (T2DM), C group (CP), D group (DM + CP). After developing the model, blood samples were collected from the angular vein analyze serum APN, hs-CRP, and blood lipid. the carotid artery was isolated for HE staining. Result Compared with group A, the serum APN in group B, C and D decreased gradually with the progression of the disease. Serum hs-CRP in group B, C and D was significantly increased. At T3, T4 and T5 in group B, C and D, APN/hs-CRP significantly decreased. TC, LDL and TG significantly increased in group B, D; HDL significantly decreased in group C. Carotid artery HE staining showed: compared with group A, different degrees of endothelial defect, destruction of elastic fibers in the middle membrane, disorder of smooth muscle arrangement, and partial dissolution 、 fragmentation and Calcium salt deposition necrosis occurred in group B, C and D. Conclusion Enhanced systemic inflammation, decreased adiponectin level, and disorganized lipid metabolism with or without type 2 diabetes attributed to local inflammation of periodontitis can result in an imbalance of pro-inflammatory and anti-inflammatory effects. Therefore, it’s more meaningful to predict the progression of DAA with anti-inflammatory/pro-inflammatory variation.
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- 2023
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