Search

Your search keyword '"Rufino-González, Yadira"' showing total 25 results
Start Over Author "Rufino-González, Yadira"
25 results on '"Rufino-González, Yadira"'

2. Nitazoxanide Inhibits the Bifunctional Enzyme GlG6PD::6PGL of Giardia lamblia: Biochemical and In Silico Characterization of a New Druggable Target

Author

Martínez-Rosas, Víctor, primary, Hernández-Ochoa, Beatriz, additional, Morales-Luna, Laura, additional, Ortega-Cuellar, Daniel, additional, González-Valdez, Abigail, additional, Arreguin-Espinosa, Roberto, additional, Rufino-González, Yadira, additional, Calderón-Jaimes, Ernesto, additional, Castillo-Rodríguez, Rosa Angélica, additional, Wong-Baeza, Carlos, additional, Baeza-Ramírez, Isabel, additional, Pérez de la Cruz, Verónica, additional, Vidal-Limón, Abraham, additional, and Gómez-Manzo, Saúl, additional

Published
2023
Full Text
View/download PDF

4. Pyridyl Methylsulfinyl Benzimidazole Derivatives as Promising Agents against Giardia lamblia and Trichomonas vaginalis

Author

Hernández-Ochoa, Beatriz, primary, Martínez-Rosas, Víctor, additional, Morales-Luna, Laura, additional, Calderón-Jaimes, Ernesto, additional, Rocha-Ramírez, Luz María, additional, Ortega-Cuellar, Daniel, additional, Rufino-González, Yadira, additional, González-Valdez, Abigail, additional, Arreguin-Espinosa, Roberto, additional, Enríquez-Flores, Sergio, additional, Castillo-Rodríguez, Rosa Angélica, additional, Cárdenas-Rodríguez, Noemí, additional, Wong-Baeza, Carlos, additional, Baeza-Ramírez, Isabel, additional, and Gómez-Manzo, Saúl, additional

Published
2022
Full Text
View/download PDF

5. Giardia lamblia G6PD::6PGL Fused Protein Inhibitors Decrease Trophozoite Viability: A New Alternative against Giardiasis

Author

Morales-Luna, Laura, primary, Hernández-Ochoa, Beatriz, additional, Martínez-Rosas, Víctor, additional, Navarrete-Vázquez, Gabriel, additional, Ortega-Cuellar, Daniel, additional, Rufino-González, Yadira, additional, González-Valdez, Abigail, additional, Arreguin-Espinosa, Roberto, additional, Franco-Vásquez, Adrián Marcelo, additional, Pérez de la Cruz, Verónica, additional, Enríquez-Flores, Sergio, additional, Martínez-Conde, Carlos, additional, Canseco-Ávila, Luis Miguel, additional, Gómez-Chávez, Fernando, additional, and Gómez-Manzo, Saúl, additional

Published
2022
Full Text
View/download PDF

7. Discovery of Benzopyrrolizidines as Promising Antigiardiasic Agents

Author

Auriostigue-Bautista, Juan Carlos, primary, Hernández-Vázquez, Eduardo, additional, González-Calderón, David, additional, Figueroa-Romero, Jorge Luís, additional, Castillo-Villanueva, Adriana, additional, Torres-Arroyo, Angélica, additional, Ponce-Macotela, Martha, additional, Rufino-González, Yadira, additional, Martínez-Gordillo, Mario, additional, Miranda, Luis D., additional, Oria-Hernández, Jesús, additional, and Reyes-Vivas, Horacio, additional

Published
2022
Full Text
View/download PDF

11. Characterizing the Fused TvG6PD::6PGL Protein from the Protozoan Trichomonas vaginalis, and Effects of the NADP+ Molecule on Enzyme Stability

Author

Morales-Luna, Laura, primary, Hernández-Ochoa, Beatriz, additional, Ramírez-Nava, Edson, additional, Martínez-Rosas, Víctor, additional, Ortiz-Ramírez, Paulina, additional, Fernández-Rosario, Fabiola, additional, González-Valdez, Abigail, additional, Cárdenas-Rodríguez, Noemí, additional, Serrano-Posada, Hugo, additional, Centeno-Leija, Sara, additional, Arreguin-Espinosa, Roberto, additional, Cuevas-Cruz, Miguel, additional, Ortega-Cuellar, Daniel, additional, Pérez de la Cruz, Verónica, additional, Rocha-Ramírez, Luz, additional, Sierra-Palacios, Edgar, additional, Castillo-Rodríguez, Rosa, additional, Vega-García, Vanesa, additional, Rufino-González, Yadira, additional, Marcial-Quino, Jaime, additional, and Gómez-Manzo, Saúl, additional

Published
2020
Full Text
View/download PDF

12. Identification of the NADP+ Structural Binding Site and Coenzyme Effect on the Fused G6PD::6PGL Protein from Giardia lamblia

Author

Morales-Luna, Laura, primary, González-Valdez, Abigail, additional, Sixto-López, Yudibeth, additional, Correa-Basurto, José, additional, Hernández-Ochoa, Beatriz, additional, Cárdenas-Rodríguez, Noemí, additional, Castillo-Rodríguez, Rosa Angélica, additional, Ortega-Cuellar, Daniel, additional, Arreguin-Espinosa, Roberto, additional, Pérez de la Cruz, Verónica, additional, Serrano-Posada, Hugo, additional, Centeno-Leija, Sara, additional, Rocha-Ramírez, Luz María, additional, Sierra-Palacios, Edgar, additional, Montiel-González, Alba Mónica, additional, Rufino-González, Yadira, additional, Marcial-Quino, Jaime, additional, and Gómez-Manzo, Saúl, additional

Published
2019
Full Text
View/download PDF

13. Identification of the NADP+ Structural Binding Site and Coenzyme Effect on the Fused G6PD::6PGL Protein from Giardia lamblia.

Author

Morales-Luna, Laura, González-Valdez, Abigail, Sixto-López, Yudibeth, Correa-Basurto, José, Hernández-Ochoa, Beatriz, Cárdenas-Rodríguez, Noemí, Castillo-Rodríguez, Rosa Angélica, Ortega-Cuellar, Daniel, Arreguin-Espinosa, Roberto, de la Cruz, Verónica Pérez, Serrano-Posada, Hugo, Centeno-Leija, Sara, Rocha-Ramírez, Luz María, Sierra-Palacios, Edgar, Montiel-González, Alba Mónica, Rufino-González, Yadira, Marcial-Quino, Jaime, and Gómez-Manzo, Saúl

Subjects
BINDING sites, GIARDIA lamblia, PENTOSE phosphate pathway, PROTEIN stability, MOLECULAR docking, TRYPSIN
Abstract

Giardia lambia is a flagellated protozoan parasite that lives in the small intestine and is the causal agent of giardiasis. It has been reported that G. lamblia exhibits glucose-6-phosphatedehydrogenase (G6PD), the first enzyme in the pentose phosphate pathway (PPP). Our group work demonstrated that the g6pd and 6pgl genes are present in the open frame that gives rise to the fused G6PD::6PGL protein; where the G6PD region is similar to the 3D structure of G6PD in Homo sapiens. The objective of the present work was to show the presence of the structural NADP+ binding site on the fused G6PD::6PGL protein and evaluate the e ect of the NADP+ molecule on protein stability using biochemical and computational analysis. A protective e ect was observed on the thermal inactivation, thermal stability, and trypsin digestions assays when the protein was incubated with NADP+. By molecular docking, we determined the possible structural-NADP+ binding site, which is located between the Rossmann fold of G6PD and 6PGL. Finally, molecular dynamic (MD) simulation was used to test the stability of this complex; it was determined that the presence of both NADP+ structural and cofactor increased the stability of the enzyme, which is in agreement with our experimental results. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

14. Biochemical Characterization and Structural Modeling of Fused Glucose-6-Phosphate Dehydrogenase-Phosphogluconolactonase from Giardia lamblia

Author

Morales-Luna, Laura, primary, Serrano-Posada, Hugo, additional, González-Valdez, Abigail, additional, Ortega-Cuellar, Daniel, additional, Vanoye-Carlo, America, additional, Hernández-Ochoa, Beatriz, additional, Sierra-Palacios, Edgar, additional, Rufino-González, Yadira, additional, Castillo-Rodríguez, Rosa, additional, Pérez de la Cruz, Verónica, additional, Moreno-Vargas, Liliana, additional, Prada-Gracia, Diego, additional, Marcial-Quino, Jaime, additional, and Gómez-Manzo, Saúl, additional

Published
2018
Full Text
View/download PDF

15. RNAi-Mediated Specific Gene Silencing as a Tool for the Discovery of New Drug Targets in Giardia lamblia; Evaluation Using the NADH Oxidase Gene

Author

Marcial-Quino, Jaime, primary, Gómez-Manzo, Saúl, additional, Fierro, Francisco, additional, Rufino-González, Yadira, additional, Ortega-Cuellar, Daniel, additional, Sierra-Palacios, Edgar, additional, Vanoye-Carlo, America, additional, González-Valdez, Abigail, additional, Torres-Arroyo, Angélica, additional, Oria-Hernández, Jesús, additional, and Reyes-Vivas, Horacio, additional

Published
2017
Full Text
View/download PDF

17. In vitro and in vivo cysticidal activity of extracts and isolated flavanone from the bark of Prunus serotina : A bio-guided study

Author

Palomares-Alonso, Francisca, primary, Rojas-Tomé, Irma Susana, additional, Palencia Hernández, Guadalupe, additional, Jiménez-Arellanes, María Adelina, additional, Macías-Rubalcava, Martha Lydia, additional, González-Maciel, Angélica, additional, Ramos-Morales, Andrea, additional, Santiago-Reyes, Rosalba, additional, Castro, Nelly, additional, González-Hernández, Iliana, additional, Rufino-González, Yadira, additional, and Jung-Cook, Helgi, additional

Published
2017
Full Text
View/download PDF

19. Stem-Loop RT-qPCR as an Efficient Tool for the Detection and Quantification of Small RNAs in Giardia lamblia

Author

Marcial-Quino, Jaime, primary, Gómez-Manzo, Saúl, additional, Fierro, Francisco, additional, Vanoye-Carlo, America, additional, Rufino-González, Yadira, additional, Sierra-Palacios, Edgar, additional, Castillo-Villanueva, Adriana, additional, Castillo-Rodríguez, Rosa, additional, Rodríguez-Bustamante, Eduardo, additional, Arreguin-Espinosa, Roberto, additional, and Reyes-Vivas, Horacio, additional

Published
2016
Full Text
View/download PDF

23. Oregano (Lippiaspp.) kills Giardia intestinalistrophozoites in vitro: antigiardiasic activity and ultrastructural damage

Author

Ponce-Macotela, Martha, Rufino-González, Yadira, González-Maciel, Angélica, Reynoso-Robles, Rafael, and Martínez-Gordillo, Mario

Abstract

In the world, giardiosis is still a very important parasitic disease; only in Asia, Africa and America, there are more than 200 million of infected people in a year. The usual treatments are drugs that produce undesirable secondary effects, perhaps favouring the resistant strain selection. One alternative is to research compounds from plants used as antidiarrhoeic or antiparasitic in the traditional medicine. In a previous work, we found that Lippia beriandieri(Oregano) revealed to be more potent than tinidazole, a common antigiardiasic drug. In this current work, we tested the cell viability by re-culture and reduction of MTT-tetrazolium salts to MTT-formazan, and we showed the effect of oregano ethanolic extracts against Giardia intestinalis(synonyms: Giardia duodenalis, Giardia lamblia) trophozoites at concentrations ranging form 58 to 588 μg. We demonstrated the ultrastructural injury produced by oregano extracts in this parasite. Trophozoites lost their size and shape and showed damage in nucleus structure, perhaps by breaking the pattern of nucleoskeleton proteins.In the world, giardiosis is still a very important parasitic disease; only in Asia, Africa and America, there are more than 200 million of infected people in a year. The usual treatments are drugs that produce undesirable secondary effects, perhaps favouring the resistant strain selection. One alternative is to research compounds from plants used as antidiarrhoeic or antiparasitic in the traditional medicine. In a previous work, we found that Lippia beriandieri(Oregano) revealed to be more potent than tinidazole, a common antigiardiasic drug. In this current work, we tested the cell viability by re-culture and reduction of MTT-tetrazolium salts to MTT-formazan, and we showed the effect of oregano ethanolic extracts against Giardia intestinalis(synonyms: Giardia duodenalis, Giardia lamblia) trophozoites at concentrations ranging form 58 to 588 μg. We demonstrated the ultrastructural injury produced by oregano extracts in this parasite. Trophozoites lost their size and shape and showed damage in nucleus structure, perhaps by breaking the pattern of nucleoskeleton proteins.

Published
2006
Full Text
View/download PDF

24. Characterizing the Fused TvG6PD::6PGL Protein from the Protozoan Trichomonas vaginalis , and Effects of the NADP + Molecule on Enzyme Stability.

Author

Morales-Luna L, Hernández-Ochoa B, Ramírez-Nava EJ, Martínez-Rosas V, Ortiz-Ramírez P, Fernández-Rosario F, González-Valdez A, Cárdenas-Rodríguez N, Serrano-Posada H, Centeno-Leija S, Arreguin-Espinosa R, Cuevas-Cruz M, Ortega-Cuellar D, Pérez de la Cruz V, Rocha-Ramírez LM, Sierra-Palacios E, Castillo-Rodríguez RA, Vega-García V, Rufino-González Y, Marcial-Quino J, and Gómez-Manzo S

Subjects
Amino Acid Sequence, Hydrogen-Ion Concentration, Kinetics, Models, Molecular, Protein Stability, Sequence Alignment, Temperature, Carboxylic Ester Hydrolases genetics, Enzyme Stability genetics, Glucosephosphate Dehydrogenase genetics, NADP genetics, Protozoan Proteins genetics, Recombinant Proteins genetics, Trichomonas vaginalis genetics
Abstract

This report describes a functional and structural analysis of fused glucose-6-phosphate dehydrogenase dehydrogenase-phosphogluconolactonase protein from the protozoan Trichomonas vaginalis ( T. vaginalis ). The glucose-6-phosphate dehydrogenase ( g6pd ) gene from T . vaginalis was isolated by PCR and the sequence of the product showed that is fused with 6pgl gene. The fused Tv g6pd :: 6pgl gene was cloned and overexpressed in a heterologous system. The recombinant protein was purified by affinity chromatography, and the oligomeric state of the TvG6PD::6PGL protein was found as tetramer, with an optimal pH of 8.0. The kinetic parameters for the G6PD domain were determined using glucose-6-phosphate (G6P) and nicotinamide adenine dinucleotide phosphate (NADP + ) as substrates. Biochemical assays as the effects of temperature, susceptibility to trypsin digestion, and analysis of hydrochloride of guanidine on protein stability in the presence or absence of NADP + were performed. These results revealed that the protein becomes more stable in the presence of the NADP + . In addition, we determined the dissociation constant for the binding ( K d ) of NADP + in the protein and suggests the possible structural site in the fused TvG6PD::6PGL protein. Finally, computational modeling studies were performed to obtain an approximation of the structure of TvG6PD::6PGL. The generated model showed differences with the GlG6PD::6PGL protein (even more so with human G6PD) despite both being fused.

Published
2020
Full Text
View/download PDF

25. Identification of the NADP + Structural Binding Site and Coenzyme Effect on the Fused G6PD::6PGL Protein from Giardia lamblia .

Author

Morales-Luna L, González-Valdez A, Sixto-López Y, Correa-Basurto J, Hernández-Ochoa B, Cárdenas-Rodríguez N, Castillo-Rodríguez RA, Ortega-Cuellar D, Arreguin-Espinosa R, Pérez de la Cruz V, Serrano-Posada H, Centeno-Leija S, Rocha-Ramírez LM, Sierra-Palacios E, Montiel-González AM, Rufino-González Y, Marcial-Quino J, and Gómez-Manzo S

Subjects
Binding Sites, Glucosephosphate Dehydrogenase metabolism, Humans, Models, Molecular, Phosphogluconate Dehydrogenase metabolism, Protein Conformation, Protein Stability, Temperature, Giardia lamblia enzymology, Glucosephosphate Dehydrogenase chemistry, NADP chemistry, NADP metabolism, Phosphogluconate Dehydrogenase chemistry
Abstract

Giardia lambia is a flagellated protozoan parasite that lives in the small intestine and is the causal agent of giardiasis. It has been reported that G. lamblia exhibits glucose-6-phosphate dehydrogenase (G6PD), the first enzyme in the pentose phosphate pathway (PPP). Our group work demonstrated that the g6pd and 6pgl genes are present in the open frame that gives rise to the fused G6PD::6PGL protein; where the G6PD region is similar to the 3D structure of G6PD in Homo sapiens . The objective of the present work was to show the presence of the structural NADP + binding site on the fused G6PD::6PGL protein and evaluate the effect of the NADP + molecule on protein stability using biochemical and computational analysis. A protective effect was observed on the thermal inactivation, thermal stability, and trypsin digestions assays when the protein was incubated with NADP + . By molecular docking, we determined the possible structural-NADP + binding site, which is located between the Rossmann fold of G6PD and 6PGL. Finally, molecular dynamic (MD) simulation was used to test the stability of this complex; it was determined that the presence of both NADP + structural and cofactor increased the stability of the enzyme, which is in agreement with our experimental results.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results