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Spotted wing drosophila, Drosophila suzukii, has become a major pest of soft‐skinned fruit crops in the Americas, Europe and North Africa. In many regions, D. suzukii management is complicated by the fly's ability to persist on non‐crop hosts and then invade or reinvade crop fields from surrounding natural habitats. In California, non‐crop habitats adjacent to commercial cane berry fields often contain wild blackberry (Rubus spp.), a potential overwintering host for D. suzukii. We sampled D. suzukii and its naturally occurring parasitoids (the pupal parasitoids Trichopria drosophilae and Pachycrepoideus vindemmiae) in commercial berry fields and adjacent non‐crop habitat throughout the year, to determine whether non‐crop habitat is a seasonal or year‐round refuge for D. suzukii, and whether numbers of D. suzukii and its parasitoids in berry fields are dependent on the composition of the surrounding landscape. Numbers of D. suzukii adults were consistently higher in non‐crop habitat, and proportions of female D. suzukii were highest in berry fields, indicating that females dispersed more widely than males. The effect of habitat diversity on numbers of D. suzukii adults was positive in fall and negative in early spring, possibly reflecting differing habitat requirements of D. suzukii over time. Parasitoid numbers were also higher in non‐crop habitat and were positively affected by proportions of non‐crop habitat and cropland in summer. Results indicate that non‐crop habitats were a year‐round refuge for both D. suzukii adults and its parasitoids, and that landscape effects on D. suzukii abundance in crop fields can change over the seasons. [ABSTRACT FROM AUTHOR]

Blackberry is grown in a wide range of soil pH, and it is known that the adequacy of soil pH is essential for plants to express their maximum nutritional efficiencies and, consequently, reach their maximum productive potential. Thus, the objective was to evaluate the nutritional accumulations and efficiencies for maximum fruit production. The experiment under greenhouse conditions was carried out in a randomized block design followed by a 4 × 2 × 4 factorial scheme with three repetitions. The evaluated factors were four blackberry cultivars (Tupy, Brazos, Guarani, and Xavante) grown in two types of acidic soils (Typical Hapludox [TH] and Rhodic Hapludox [RH]) to which four rates of dolomitic limestone (0, 1.5, 3.0, and 6.0 Mg ha−1) were applied. Fruit production and nutrient accumulation in the fruits, leaves, stems, and roots of the plants were evaluated, as well as the nutritional efficiency of each cultivar. The blackberry cultivar Tupy has been the most productive, independent of the liming rate. Blackberry cultivars have shown differences in nutrient accumulation and efficiencies for maximum fruit production, and they also have distinct nutritional requirements. [ABSTRACT FROM AUTHOR]

Plant growth promoting rhizobacteria (PGPR) play an important role in crop production by improving plant growth through various mechanisms that potentially increase sustainable agriculture. Blackberry (Rubus spp.) cultivation has acquired importance for its worldwide merchandising, including its high economic profitability, and for the health benefits in human consumption of the fruit. The quality of blackberry production depends on a good vegetative plant development; therefore, this research objective was to select strains of PGPR that stimulate vegetative growth of blackberry plants grown under greenhouse. The experiment was carried out using seedlings of the 'Tupy' blackberry, under a completely randomized design, using six rhizobacterial strains as treatments (A46, AC-35, P61, R44, BSP1.1, JLB4) and a control (no inoculated) with six replicates each. The plants were in the greenhouse for 78 d. At destructive sampling eight agronomical variables were determined. The results showed that strains A46 and P61 inoculated on blackberry plants stimulated height (45%), stem diameter (17%), leaf area (110%), and shoot DM (150%). Inoculation of strain A46 on blackberry plants increased nutrient extraction of N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, and Zn. Pseudomonas tolaasii strains P61 and A46 stimulated growth and nutrient extraction in blackberry plants, and these will be selected to inoculate the blackberry crop for production under greenhouse conditions. [ABSTRACT FROM AUTHOR]

Interactions between microorganisms and frugivorous insects can modulate fruit rot disease epidemiology. Insect feeding and/or oviposition wounds may create opportunities for fungal infection. Passive and active dispersal of fungal inoculums by adult insects also increases disease incidence. In fall-bearing raspberries and blackberries, such vectoring interactions could increase crop damage from the invasive pestiferous vinegar fly Drosophila suzukii (spotted-wing drosophila). Periods of peak D. suzukii activity are known to overlap with several species of primary fruit rot pathogen, particularly Botrytis cinerea and Cladosporium cladosporioides, and previous work indicates that larvae co-occur with and feed on various filamentous fungi at low rates. To further our understanding of the epidemiological consequences that may emerge from these associations, we surveyed the filamentous fungal community associated with adult D. suzukii, isolating and molecularly identifying fungi externally and internally (indicating feeding) from field-collected adults over 3 years. We isolated and identified 37 unique genera of fungi in total, including known raspberry pathogens. Most fungi were detected infrequently, and flies acquired and carried fungi externally at higher richness, frequency, and density relative to internally. In a worst-case scenario laboratory vectoring assay, D. suzukii adults were able to transfer B. cinerea and C. cladosporioides to sterile media at 0, 24, 48, and 72 h after exposure to sporulating cultures in Petri dishes. These results collectively suggest an adventitious vectoring association between D. suzukii and fruit rot fungi that has the potential to alter caneberry disease dynamics. [ABSTRACT FROM AUTHOR]

Reliable and high-throughput genotyping platforms are of immense importance for identifying and dissecting genomic regions controlling important phenotypes, supporting selection processes in breeding programs, and managing wild populations and germplasm collections. Amongst available genotyping tools, single nucleotide polymorphism arrays have been shown to be comparatively easy to use and generate highly accurate genotypic data. Single-species arrays are the most commonly used type so far; however, some multi-species arrays have been developed for closely related species that share single nucleotide polymorphism markers, exploiting inter-species cross-amplification. In this study, the suitability of a multiplexed plant-animal single nucleotide polymorphism array, including both closely and distantly related species, was explored. The performance of the single nucleotide polymorphism array across species for diverse applications, ranging from intra-species diversity assessments to parentage analysis, was assessed. Moreover, the value of genotyping pooled DNA of distantly related species on the single nucleotide polymorphism array as a technique to further reduce costs was evaluated. Single nucleotide polymorphism performance was generally high, and species-specific single nucleotide polymorphisms proved suitable for diverse applications. The multi-species single nucleotide polymorphism array approach reported here could be transferred to other species to achieve cost savings resulting from the increased throughput when several projects use the same array, and the pooling technique adds another highly promising advancement to additionally decrease genotyping costs by half. [ABSTRACT FROM AUTHOR]

ABSTRACT Blackberries have a short post-harvest conservation period, making it necessary to optimize harvest and storage conditions. The objective of this study was to evaluate the impact of refrigerated storage and maturation stage on the physicochemical quality of ‘BRS Cainguá’ blackberries, produced in an organic system. Blackberries from an experimental orchard, were harvested at three ripeness stages (RS), with RS1 being fruits with 100% red skin; RS2 skin 50% red and 50% black; and EM3 100% black skin. The samples were stored in a cold chamber at 4±0.5ºC and 90-95% RH for 12 days. The evaluations were carried out after 0, 3, 6, 9 and 12 days of storage, for: weight loss (WL), soluble solids (SS), pH, titratable acidity (TA), ratio (SS/TA) and color parameters. Blackberries harvested in RS3 had the highest SS content, lowest TA and the highest SS/TA ratio and pH. During storage, TA and WL showed decreasing and increasing linear responses, respectively, regardless of the RS of the fruits. Harvesting based on the skin color of ‘BRS Cainguá’ blackberries influences the physicochemical quality and postharvest conservation. Fruits harvested with 100% black skin, have the best quality and are the most suitable for subsequent refrigerated storage.

Blackberries have a short post-harvest conservation period, making it necessary to optimize harvest and storage conditions. The objective of this study was to evaluate the impact of refrigerated storage and maturation stage on the physicochemical quality of 'BRS Cainguá' blackberries, produced in an organic system. Blackberries from an experimental orchard, were harvested at three ripeness stages (RS), with RS1 being fruits with 100% red skin; RS2 skin 50% red and 50% black; and EM3 100% black skin. The samples were stored in a cold chamber at 4±0.5ºC and 90-95% RH for 12 days. The evaluations were carried out after 0, 3, 6, 9 and 12 days of storage, for: weight loss (WL), soluble solids (SS), pH, titratable acidity (TA), ratio (SS/TA) and color parameters. Blackberries harvested in RS3 had the highest SS content, lowest TA and the highest SS/TA ratio and pH. During storage, TA and WL showed decreasing and increasing linear responses, respectively, regardless of the RS of the fruits. Harvesting based on the skin color of 'BRS Cainguá' blackberries influences the physicochemical quality and postharvest conservation. Fruits harvested with 100% black skin, have the best quality and are the most suitable for subsequent refrigerated storage. [ABSTRACT FROM AUTHOR]

Blueberries and blackberries are highly valued and increasing in demand over the last twenty years, despite facing challenges like water loss, shriveling, softening, and vulnerability to phytopathogens, impacting their storage and marketability. Thus, postharvest quality preservation is a crucial area of interest for the small fruit industry. In this study, blackberries and blueberries were packed in modified humidity clamshells (named as LV, for low-vented), with an opening ratio 6.43 times lower than that in commercially available clamshells (COM). Also, the antifungal effect of the thymol, an essential oil, microencapsulated in a pectin-alginate matrix as a controlled-release system was assayed. Results show that relative humidity within LV clamshells remained higher and less variable than in COM clamshells, which led to significantly lower weight loss in both crops. Blueberry firmness, shriveling and marketability were positively impacted by storage in LV clamshells, and the inclusion of a microencapsulated thymol sachet successfully reduced postharvest decay. Our results indicate that these two tools could be used to extend blueberry shelf life, with their combination yielding the most optimal results. However, the same strategies were ineffective for blackberry quality maintenance. After exposure to thymol, more decay was observed, whereas firmness, shrivel and marketability were not affected either by clamshell vent area or thymol treatment. This underscores the importance of tailoring storage conditions, packaging design, and handling methods to each specific fruit type. • LV clamshells held higher and less variable relative humidity than COM clamshells. • The use of LV clamshells significantly reduced berries weight and firmness loss. • Blueberries shriveling and marketability were positively impacted by LV clamshells. • Microencapsulated thymol successfully alleviated natural decay on blueberries. • LV clamshells and thymol treatment were ineffective for blackberries preservation. [ABSTRACT FROM AUTHOR]

A Rosaceae family-level candidate gene approach was used to identify genes associated with sugar content in blackberry (Rubus subgenus Rubus). Three regions conserved among apple (Malus × domestica), peach (Prunus persica), and alpine strawberry (Fragaria vesca) were identified that contained previously detected sweetness-related quantitative trait loci (QTL) in at least two of the crops. Sugar related genes from these conserved regions and 789 sugar-associated apple genes were used to identify 279 Rubus candidate transcripts. A Hyb-Seq approach was used in conjunction with PacBio sequencing to generate haplotype level sequence information of sugar-related genes for 40 cultivars with high and low soluble solids content from the University of Arkansas and USDA blackberry breeding programs. Polymorphisms were identified relative to the ‘Hillquist’ blackberry (R. argutus) and ORUS 4115-3 black raspberry (R. occidentalis) genomes and tested for their association with soluble solids content (SSC). A total of 173 alleles were identified that were significantly (α = 0.05) associated with SSC. KASP genotyping was conducted for 92 of these alleles on a validation set of blackberries from each breeding program and 48 markers were identified that were significantly associated with SSC. One QTL, qSSC-Ruh-ch1.1, identified in both breeding programs accounted for an increase of 1.5 °Brix and the polymorphisms were detected in the intron space of a sucrose synthase gene. This discovery represents the first environmentally stable sweetness QTL identified in blackberry. The approach demonstrated in this study can be used to develop breeding tools for other crops that have not yet benefited directly from the genomics revolution.

Apesar de proporcionar rápido retorno econômico e estar bastante ajustada a agricultura familiar, o cultivo de amora-preta no Rio Grande do Sul encontra limitações pela carência de informação sobre seu manejo de poda. O trabalho teve por objetivo identificar a influência da época de poda no ciclo fenológico, produção e qualidade dos frutos de amora-preta. Plantas da cultivar 'Tupy' foram submetidas a três épocas de poda: precoce (18/7), convencional (10/8) e tardia (01/9). O delineamento foi em blocos casualizados, com dez repetições e seis plantas por parcela. As plantas tiveram acompanhamento visual das principais fases do ciclo fenológico com avaliação do tamanho e número de frutos, teor de sólidos solúveis totais, produção e produtividade estimada. Com a poda tardia, a brotação foi adiada em 11 dias e o início e o fim da colheita foram adiados em 10 dias. O teor de sólidos solúveis total, massa, e tamanho dos frutos não são afetados pela época de poda, no entanto, comparado a época normal de poda as plantas que receberam a poda tardia produziram 107 frutos a menos por planta, 740 g a menos na produção individual por planta e 4.229 kg ha-1 a menos na produtividade estimada.

The study aimed to evaluate the effect of pruning intensity on the phenology and production of blackberry cv. Xavante. The experiment was carried out at the Federal University of Paraná - Palotina, PR, Brazil, during the 2018/2019 season. The completely randomized design was used as a statical model with four treatments and seven replications. The treatments consisted of different number of stems of 1 m height retained per bush after the pruning: 2, 4, 6, and 8, and each plot consisted of one bush. Data collection included: sprouting and flowering (%), number of fruits per bush, fresh weight, total soluble solids content and titratable acidity of fruits. The number of stems per bush significantly influenced the number of shoots, flowers and fruits per bush, titratable acidity, soluble solids and maturation index. Under the growth conditions, the treatments consisted of 2 and 4 stems per bush resulted in more uniform growth development and higher chemical quality of fruits. However, the highest number of fruits per bush was observed when 8 stems per bush were retained. [ABSTRACT FROM AUTHOR]

In most plants, sucrose is the predominant form of carbohydrate for long-distance transport, and sucrose transporters (SUTs) mediate its transportation. In present study, we cloned a new sucrose transporter gene RuSUT2 from blackberry (Rubus spp.) cultivar Boysenberry fruit. The RuSUT2 cDNA contained an open reading frame (ORF) of 1986 nucleotides encoding a 631 amino acids protein. It was predicted that the protein exhibited 11 transmembrane spanning domain and the histidine residue (H) was conserved in the extracellular loop. Subcellular localization investigation indicated that RuSUT2 protein was localized in the plasma membrane. The phylogenetic comparisons revealed that RuSUT2 was a member of SUT2 clade and shared high identity with RcSUC3 in Rosa chinensis, FvSUC3 in Fragaria vesca, and PpSUT2 in Prunus persica. The quantitative real-time PCR (qRT-PCR) was performed to investigate the expression patterns of RuSUT2. The expression of RuSUT2 was detected in all test organs, and showed much higher expression in roots and leaves. During the fruit ripening stage, RuSUT2 was highly expressed at the 33 days after flowering (DAF). Overexpression of RuSUT2 enhanced sucrose content in the mature leaves of the transgenic tobacco. Our findings suggested that RuSUT2 has transport activity and could be involved in the sucrose transportation during blackberry plant growth and development. [ABSTRACT FROM AUTHOR]

A Rosaceae family-level candidate gene approach was used to identify genes associated with sugar content in blackberry (Rubus subgenus Rubus). Three regions conserved among apple (Malus · domestica), peach (Prunus persica), and alpine strawberry (Fragaria vesca) were identified that contained previously detected sweetness-related quantitative trait loci (QTL) in at least two of the crops. Sugar related genes from these conserved regions and 789 sugar-associated apple genes were used to identify 279 Rubus candidate transcripts. A Hyb-Seq approach was used in conjunction with PacBio sequencing to generate haplotype level sequence information of sugar-related genes for 40 cultivars with high and low soluble solids content from the University of Arkansas and USDA blackberry breeding programs. Polymorphisms were identified relative to the ‘Hillquist’ blackberry (R. argutus) and ORUS 4115-3 black raspberry (R. occidentalis) genomes and tested for their association with soluble solids content (SSC). A total of 173 alleles were identified that were significantly (a = 0.05) associated with SSC. KASP genotyping was conducted for 92 of these alleles on a validation set of blackberries from each breeding program and 48 markers were identified that were significantly associated with SSC. One QTL, qSSCRuh-ch1.1, identified in both breeding programs accounted for an increase of 1.5 )Brix and the polymorphisms were detected in the intron space of a sucrose synthase gene. This discovery represents the first environmentally stable sweetness QTL identified in blackberry. The approach demonstrated in this study can be used to develop breeding tools for other crops that have not yet benefited directly from the genomics revolution. [ABSTRACT FROM AUTHOR]

Key message: This study showed that regeneration success (presence of oaks ≥ 150 cm in total height) in artificial canopy openings of a mature mixed sessile oak stand was mainly driven by initial oak seedling density. Context: Small-scale harvesting methods as practiced in close-to-nature forestry may disadvantage the regeneration of more light-demanding tree species including sessile oak (Quercus petraea [Mattuschka] Liebl.) and thus cause regeneration failure. However, owing to the short-term nature of many previous studies, regeneration success of sessile oak could not be properly ascertained. Aims: This study examined oak seedling development over a time period of ten growing seasons in canopy openings of 0.05 to 0.2 ha in size created through group selection harvesting in a mature mixed sessile oak forest in southwestern Germany. We tried to answer the following research questions: (i) how do initial stand conditions relate to and interact with oak seedling density and seedling height growth, and (ii) what are the driving factors of regeneration success under the encountered site conditions. Methods: We evaluated the influence of solar radiation, Rubus spp. cover, initial oak seedling density, and competition from other tree species on change in density and height of oak seedlings, as well as overall regeneration success (oak seedlings ≥ 150 cm in height). Results: Regeneration success increased with initial oak seedling density and solar radiation levels and decreased with early Rubus spp. cover. Density and maximum height of oak seedlings was negatively related with competition of other woody species. Conclusion: Results of our longer-term study demonstrate that forest management activities to regenerate sessile oak naturally are only successful in stands (i) without advance regeneration of other woody species and without established, recalcitrant ground vegetation, (ii) with a sufficiently high initial oak seedling density in larger patches following mast years, and (iii) where periodic monitoring and control of competing woody individuals can be ensured. Our findings further corroborate the view that natural regeneration of sessile oak in small-scale canopy openings is possible in principle. [ABSTRACT FROM AUTHOR]

The demand for blackberry has been increasing due to the results of studies on its nutraceutical properties. Moreover, the rusticity of its plants allows its cultivation with reduced use of pesticides, becoming feasible to grow it in areas of environmental protection (APA). As a non-traditional crop in the country and its requirement in specific climatic conditions, there is a lack of studies about management techniques and the adaptability of cultivars in different regions. Thus, it is necessary to study phenological behavior and fruit quality in potential regions. Therefore, this work aimed to verify the adaptability of blackberry cultivars in environmental protection area in Pinhais - PR (Brazil), with no use of pesticides, as well as to evaluate the quality of the fruits produced under this condition, focusing on the recommendation of the cultivars more adapted and the best destination of fruits produced. For this, the phenological and productive performance of seven blackberry cultivars: Tupy, Guarani, Cherokee, Xavante, Brazos, Choctaw, and Arapaho were observed during the cycles of 2017/2018 and 2018/2019. Brazos cultivar had the earliest harvest period, Guarani and Xavante cultivars were intermediate and Tupy and Cherokee cultivars were later in the region. The highest yields were obtained with the cultivars Tupy, Guarani, Xavante, Cherokee, and Brazos. The harvest period extended from November to January. Tupy cultivar produces larger fruits and is the most recommended for marketing as fresh fruit. Other cultivars are recommended for industrialization. [ABSTRACT FROM AUTHOR]

Tocopherol (vitamin E) is widely recognized as a cellular antioxidant and can prevent oxidative damage during seed quiescence and dormancy in all angiosperms. Tocopherols biosynthesis was highly accumulated in blackberry seeds, but limited study was done on expression of critical genes involved in this biosynthesis pathway. In the present study, each RuHPT and RuTMT cDNA sequence was isolated from boysenberry (Rubus idaeus × fruticosus × loganobaccus Darrow) and their expression patterns and regulation of tocopherols contents were investigated during different seed development stages from 14 to 42 days. RuHPT contained an open reading frame (ORF) of 1224 bp that encoded 407 amino acid residues, and for RuTMT, an ORF of 1056 bp encoding 351 amino acid residues. Phylogenetic analysis indicated that RuHPT and RuTMT were closely related to other Rosaceae species, Rosa chinensis and Fragaria vesca, respectively. The transcript level of both RuHPT and RuTMT gradually increased and reached a maximum level in the seeds of late ripening stages, 35 days after flowering (DAF) and 42 DAF. Assay of total tocopherol, α-tocopherol, γ‑tocopherol and δ-tocopherol contents by HPLC all revealed a similar changing trend concomitantly with the rising expression of RuHPT, and reached the peak on Day 35. The α-tocopherol level showed itself a trend of descending after ascending and reached its peak on Day 28. Therefore, RuHPT and RuTMT may both play a role in tocopherols biosynthesis in the seeds of ripening fruits. These results provide a novel foundation for investigating the functions of key enzyme genes on tocopherols biosynthesis in blackberry plants. [ABSTRACT FROM AUTHOR]

Calcium chloride application on fruits maintains the firmness of fruits, decreases storage breakdown and rot, extends shelf-life, and increases vitamin C and calcium content. Blackberries have low post-harvest durability due to the intrinsic characteristics of their fruits, mainly the high respiratory rate and low firmness, which causes problems in the production chain of fresh fruits. The current study aimed to evaluate the effects of pre-harvest application of calcium chloride on the fruits of ‘Tupy’ blackberry (Rubus spp.). A randomized block design for a factorial scheme was used, i.e. calcium chloride concentrations (0, 1.5, 3, and 4.5%) and number of applications (1, 2, and 3). Multiple applications occurred between 5-day intervals. The results showed that the calcium content in the fruits increased with a single application of 2.2%, but a decrease in mass loss was observed with a 4.5% application. The results also indicated great firmness and ascorbic acid content of fruits of ‘Tupy’ blackberry, in addition to the significant increase in fruit size and mass. However, in general, the content of soluble solids, sugars, antioxidant activity and total polyphenols decreased. [ABSTRACT FROM AUTHOR]

In the western region of Paraná, the subtropical climate prevails, with little accumulation of hours of cold in the winter, making the fruit species not go into complete dormancy. Given the above, the objective of this study was to evaluate the effect of chemical defoliant on blackberry varieties, aiming at their complete defoliation and induction of numbness. The work was conducted at Unioeste, Marechal Cândido Rondon Campus, in a four-year-old orchard of plants, spaced 3 x 0.5 m, simple spreader with two wire strands at a height of 120 cm from the ground, and 20 plants per grow crops. The experimental design used was randomized blocks, where two treatments related to the application of defoliant (with and without) on 7 cvs were evaluated blackberry, containing three replicates and three plants in each cultivar. Was used as a defoliant lime sulfur applied in August pruning then held 25 days after application. Regarding fruit quality, Caigangue and Tupy varieties showed the highest SS/AT ratio, indicating the best flavor of their fruits for fresh consumption. The cultivars Brazos and Guarani showed lower SS/AT ratio, indicating more acidic fruits. Higher levels of ascorbic acid were found in fruits of cvs. Guarani and Cherokee. The application of defoliant reduced the total number of blackberry fruits of the cultivars Comanche and Guarani. The use of defoliant did not interfere in the physicochemical characteristics of the blackberry fruits of the evaluated cultivars. [ABSTRACT FROM AUTHOR]

ABSTRACT Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56 days after start of the project. For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.

Calcium chloride application on fruits maintains the firmness of fruits, decreases storage breakdown and rot, extends shelf-life, and increases vitamin C and calcium content. Blackberries have low post-harvest durability due to the intrinsic characteristics of their fruits, mainly the high respiratory rate and low firmness, which causes problems in the production chain of fresh fruits. The current study aimed to evaluate the effects of pre-harvest application of calcium chloride on the fruits of ‘Tupy’ blackberry (Rubus spp.). A randomized block design for a factorial scheme was used, i.e. calcium chloride concentrations (0, 1.5, 3, and 4.5%) and number of applications (1, 2, and 3). Multiple applications occurred between 5-day intervals. The results showed that the calcium content in the fruits increased with a single application of 2.2%, but a decrease in mass loss was observed with a 4.5% application. The results also indicated great firmness and ascorbic acid content of fruits of ‘Tupy’ blackberry, in addition to the significant increase in fruit size and mass. However, in general, the content of soluble solids, sugars, antioxidant activity and total polyphenols decreased.

ABSTRACT: Blackberry is part of the small-fruit group and the blackberry cultivation area is expanding as an important option for small farmers using the agroecological production system. Mites are prominent among the world’s major crop pests, as they can affect plants from growth to fruiting. The objective of this study was to evaluate the occurrence of phytophagous and predatory mites in different blackberry genotypes in the municipality of Pelotas, RS, Brazil. This study was carried out in the period Nov. 2015 - Oct. 2016, totaling 14 samples. A total of 11,158 mites were collected [phytophagous (5940) and predatory (323) mites, and those with varied feeding habits (4895)], which were distributed in 8 families. The species Neotetranychus asper (Feres & Flechtmann, 2000), Diptacus rubuscolum (Trinidad, Duarte & Navia, 2018), and Acalitus orthomerus (Keifer, 1951) had highest occurrence. According to the faunistic classification, the N. asper phytophagous species is predominant in genotypes 128 and 178; whereas, D. rubuscolum is predominant in genotype 132. Monitoring of these genotypes is recommended to detect possible damage to the crop.

In China, Phytophthora rubi, P. fragariae and P. × cambivora are quarantine organisms affecting berries for which rapid, specific and high-throughput methods are required for their simultaneous molecular detection. In this study, universal primers 18SUF/18SUR for the tested 13 Phytophthora species based on 18S rRNA sequences, specific primers PRSF/PRSR and PFSF/PFSR respectively for P. rubi and P. fragariae based on nad9 gene sequences, and specific primers PCSF/PCSR for P. × cambivora based on Ypt1 gene sequences were designed. The specificity of primers was demonstrated using 17 strains belonging to 13 Phytophthora species. A quadruplex PCR that specifically and simultaneously detects the three quarantine pathogens was developed. Application of this system detected an 884 bp universal band of 18S rRNA among all the Phytophthora species, a specific band of 556 bp and 615 bp to nad9 gene respectively in P. rubi and P. fragariae, and a 314 bp band specific to Ypt1 gene in P. × cambivora. Over conventional PCR, the sensitivity of the quadruplex PCR for P. fragariae was unchanged and lowered for P. rubi and P. × cambivora; but the specificity and throughput of the quadruplex PCR were all increased. This study represents the first molecular differentiation of P. rubi and P. fragariae, the two very similar Phytophthora species affecting berries. The quadruplex PCR developed can be applied for the rapid, specific and simultaneous detection in culture of the three quarantine Phytophthora species affecting berries and lays an excellent basis for further research to test it directly on plant materials and /or soil samples. [ABSTRACT FROM AUTHOR]

Blackberry is part of the small-fruit group and the blackberry cultivation area is expanding as an important option for small farmers using the agroecological production system. Mites are prominent among the world's major crop pests, as they can affect plants from growth to fruiting. The objective of this study was to evaluate the occurrence of phytophagous and predatory mites in different blackberry genotypes in the municipality of Pelotas, RS, Brazil. This study was carried out in the period Nov. 2015 - Oct. 2016, totaling 14 samples. A total of 11,158 mites were collected [phytophagous (5940) and predatory (323) mites, and those with varied feeding habits (4895)], which were distributed in 8 families. The species Neotetranychus asper (Feres & Flechtmann, 2000), Diptacus rubuscolum (Trinidad, Duarte & Navia, 2018), and Acalitus orthomerus (Keifer, 1951) had highest occurrence. According to the faunistic classification, the N. asper phytophagous species is predominant in genotypes 128 and 178; whereas, D. rubuscolum is predominant in genotype 132. Monitoring of these genotypes is recommended to detect possible damage to the crop. [ABSTRACT FROM AUTHOR]

O cultivo de amoras está amplamente difundido em zonas temperadas, porém, algumas cultivares são de baixa necessidade em frio e podem ser exploradas em locais com temperaturas mais elevadas. Assim, objetivou-se, com o presente trabalho, avaliar a produção de amoras-pretas e amora-vermelha na região de Lavras-MG (clima Cwb). O delineamento experimental foi em blocos ao acaso, com quatro blocos e onze tratamentos (cultivares de amoreiras-pretas 'Arapaho', 'Xavante', 'Ébano', 'Comanche', 'Caingangue', 'Choctaw', 'Tupy', 'Guarani', 'Brazos', 'Cherokee' e a amoreira-vermelha, espécie nativa do Brasil). Em cada parcela, foram coletados dados fenológicos e produtivos no ciclo de produção 2010/11 e 2011/12, além da caracterização físico-química dos frutos. As cultivares de amoreira-preta apresentaram ciclo produtivo entre 66 e 133 dias, com colheitas se iniciando em setembro e se estendendo até janeiro; e a amoreira-vermelha com ciclo produtivo de 283 dias. 'Brazos' apresentou a maior produtividade estimada. As cultivares Brazos e Guarani possibilitaram a produção de frutos de maior massa, enquanto que 'Caingangue', 'Tupy' e 'Choctaw' apresentaram bom equilíbrio entre os sólidos solúveis e acidez. A amoreira-vermelha apresentou elevada produção de frutos (527g por planta no 1o ciclo e 344g por planta no 2o ciclo) e demonstrou-se uma excelente opção para o processamento

O objetivo deste trabalho foi avaliar o efeito da adubação nitrogenada, de plantas matrizes, no enraizamento de estacas de amoreira-preta. O experimento foi realizado na Embrapa Clima Temperado, Pelotas, RS. O delineamento experimental foi o inteiramente casualizado, em fatorial 2x5, ou seja, dois cultivares ('Tupy' e 'Xavante') e cinco doses de nitrogênio (N) (0,0; 7,5; 15,0; 22,5 e 30,0 g planta-1). Avaliaram-se a percentagem (%) de estacas enraizadas, com calo, vivas, brotadas, o comprimento da maior raiz e da maior brotação (cm), o número de raízes e de brotações por estaca. Quanto ao enraizamento e ao número de raízes por estaca verificou-se aumento linear com o acréscimo da dose de N aplicada na planta matriz, efeito contrário ao observado na variável calo. O comprimento da maior brotação, o número de raízes e de brotações, além da percentagem de estacas brotadas foram maiores no cultivar ''Xavante'' (2,68, 3,02, 1,22 e 52,08, respectivamente). Quanto à percentagem de estacas vivas destacou-se o cultivar 'Tupy' (98,55%). Por essa razão, conclui-se que o uso do nitrogênio em plantas matrizes pode ser benéfico ao enraizamento de estacas de amoreira-preta, bem como ao desenvolvimento inicial do sistema radicular e da parte aérea das novas plantas, porém com efeitos negativos aos parâmetros comprimento da maior raiz e percentagem de calo.

Fruits from temperate and tropical climates which have high levels of antioxidant compounds are the source of numerous studies concerning the correlation with benefits to human health. The objectives of this study were to quantify the anthocyanins and phenolic compounds and also to measure the antioxidant activity (ferric reducing antioxidant power - FRAP) of blackberries from two varieties grown in southern Brazil ('Brazos' and 'Tupy') at three stages of ripening; unripe, semi-ripe, ripe and their products (pulp and fermented products). During fruit ripening it was observed that weight, size, diameter and sugars increase significantly and acidity decreased significantly. The anthocyanin content ranged from 4.19 (semi-ripe 'Tupy' variety) to 205.75mg 100g-1 (ripe 'Brazos' variety). The highest levels of phenolic compounds were observed for the unripe fruit of both varieties, while antioxidant activity showed no significant difference during the ripening stages. The studied pulp showed a high content of phenolic compounds (ten times higher than that found in the ripe fruits). The anthocyanin content and antioxidant activity did not show the same increase due to the degradation of anthocyanins caused by the heat treatment that was used. The alcoholic fermented beverage made from blackberries remained stable (total phenolic compounds and antioxidant activity) during two years of storage, but the in third year a significant reduction in antioxidant activity was observed. These results can be important for establishing the shelf life of this kind of product made with blackberry

Copyright of Turkish Journal of Weed Science is the property of Gaziosmanpasa Universitesi Matbaasi, Department of Plant Protection, Faculty of Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Research was conducted during three consecutive years to evaluate the effects of compound NPK (15-15-15), cattle manure and natural zeolite, commercially named “Agrozel”, applied alone or in combination, on cane properties, yield, berry quality attributes and mid-summer leaf mineral status of ‘Thornfree’ blackberry grown on an acidic, shallow, heavy-textured soil in the region of Cacak, Western Serbia.Results revealed that all evaluated traits significantly differed among fertilizer treatments and across years in general. In most cases, all evaluated properties, except leaf nutrient content, were affected by the fertilizer treatment × year interaction. Generally, mixtures of NPK, cattle manure and Agrozel (T 3 ) and NPK and cattle manure (T 7 ), and NPK alone (T 1 ) in most cases had better and similar capacity to improve primocane growth, bearing shoot characteristics, productivity, berry physical properties and levels of some macro- and micronutrients compared to other treatments, whereas the combined applications of Agrozel and manure (T 4 ) and NPK and Agrozel (T 5 ) and especially Agrozel alone (T 6 ) induced the lowest values for most properties. The effect of fertilization treatments on berry soluble solids content (SSC), titratable acidity (TA), juice pH and leaf N, Mg and Zn levels was not significant. The highest values of cane traits, berry external attributes and RI were obtained in 2008, yield, berry acidity, most of leaf nutrients in 2010, and SSC and juice pH were found in 2009, respectively. DOP and ΣDOP indexes revealed that primocane leaf nutrient amounts were within recommended levels, except P, K and B, which were below recommended standards. Three-year fertilization induced a good leaf nutrient balance. [ABSTRACT FROM AUTHOR]

Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence of phenolic compounds. The results showed significant differences in the levels of phenolic compounds in the blackberry cultivars tested. Antioxidant activity was evaluated using the ABTS free radical and ranged from 2.7 ± 0.1 to 19 ± 2 μmole of Trolox equivalents per gram of sample (b.u.). These results are in good correlation with the phenolic contents of the blackberries tested. The Xavante blackberry cultivar had the highest levels of polyphenols that could be individually identified. Catechin polyphenols were found to be the main component in the blackberry varieties tested.

A amora-preta é uma fruta que vem despertando a atenção de produtores e consumidores devido ao seu sabor agradável, cor atrativa e por apresentar em sua composição elevado teor de compostos bioativos. O grande entrave para consumo e a comercialização dos frutos da amoreira é sua elevada taxa respiratória, o que reduz sua vida útil. Uma alternativa viável para o aproveitamento econômico dessas frutas consiste em sua industrialização, podendo ser congeladas, enlatadas, processadas na forma de polpa, ou na forma de sucos e geleias. O objetivo deste trabalho foi avaliar os efeitos da radiação gama na conservação da polpa de amora. A irradiação foi realizada no laboratório de irradiação gama do Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), em uma fonte de cobalto 60, com as seguintes doses de radiação: 0,75 kGy; 1,5 kGy e 3 kGy, sob uma taxa de dose de 3,24 kGy.h-1 . A polpa não irradiada foi utilizada como controle. As polpas de amora irradiadas foram armazenadas à temperatura de 4ºC, sendo avaliadas nos tempos de 0; 7; 15; 30 e 60 dias. Para verificar os efeitos da radiação gama no processamento na polpa, foi realizada a caracterização físico-química e química através das análises de acidez, pH, sólidos solúveis, sólidos totais, teor de antocianinas, atividade antioxidante e cor. Também foi feita análise microbiológica de acordo com a legislação brasileira vigente. O processo de irradiação aumentou a vida útil de prateleira da polpa em até 60 dias, sendo que o tratamento com uma dose de 1,5 kGy foi a que proporcionou a melhor qualidade microbiológica.

Copyright of Fruits is the property of International Society for Horticultural Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Rubus stunt, caused by ' Candidatus Phytoplasma rubi' (Rubus stunt phytoplasma; RSP), is an economically important disease of Rubus. This disease occurs in wild and cultivated Rubus spp. in Europe but has not been reported from North America; however, its major leafhopper vector is well established in western Canada and the U.S.A. RSP has the potential to impact the cane-fruit industry by significantly compromising yields and impacting export potential for Rubus propagation material. To mitigate the risk of this disease entering or establishing, import and export testing of propagation material is a phytosanitary requirement in Canada, the U.S.A., and other countries regulating RSP. In the absence of a specific test for RSP, the current testing scheme involves the use of a generic test to screen for phytoplasmas followed by additional time-consuming procedures to confirm the phytoplasma species. In this study, a real-time PCR assay, targeting a 154-bp region of tuf gene, was developed for sensitive and specific detection of RSP in Rubus spp. The developed assay detected a minimum of five target copies, and no cross-reactivity was observed even with the ' Ca. P. rubi'-related strain associated with blackberry witches' broom, which differs from RSP only by a single nucleotide polymorphism in the target region. Repeatability of the developed assay was checked on two real-time PCR platforms with acceptable results. In conclusion, this real-time PCR assay provides a sensitive and specific detection of RSP for mitigating the introduction and spread of Rubus stunt disease in Rubus spp., Competing Interests: The author(s) declare no conflict of interest.

Sensorial attributes such as color, texture, acidity and nutritional composition are essential quality components for blackberry (Rubus spp.). The mineral content and quality of fruits of different blackberry cultivars produced in Lavras, Southern Minas Gerais, in a tropical climate at an elevation (Cwb, according to Köppen) were analyzed. The analyzed minerals were phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), manganese (Mn), copper (Cu) and iron (Fe). Additionally, chemical characteristics, the total acidity (citric acid), pH, soluble solids (°Brix) and vitamin C (ascorbic acid) level, and sensory characteristics, color (L*a*b) and firmness (N) were also evaluated. According to the results, the Choctaw and Xavante blackberry cultivars demonstrated the highest mineral contents, the Caingangue cultivar showed the highest soluble solid content, and the Ebano cultivar exhibited the highest vitamin C concentration and firmer fruits. The chemical variables in the fruits of the different blackberry cultivars presented little significant correlation with the contents of macrominerals present, whereas the opposite was obtained for the micronutrients. However, the chemical characteristics combined with the nutritional characteristics can be proposed as an excellent tool for the selection of cultivars of superior quality and high nutritional value.

ABSTRACT The objective of this work was to evaluate the content of phenolic compounds, anthocyanins and flavonoids extracted with 80% ethanol and water, and antioxidant capacity (DPPH and ABTS) of five blackberry cultivars produced in Western Paraná, Brazil. Brazos, Tupy, Arapaho, Choctaw and Guarani blackberries were studied. Soluble solids, titratable acidity and ascorbic acid of fruits were also characterized. Total phenolic compounds ranged from 8.23 to 14.98 mg GAE g-1 (f.w.) and Arapaho cultivar exhibited the highest contents in both solvents. Anthocyanins ranged from 2.57 to 9.42 mg ECy3glu g-1 (f.w.), being the solvent ethanol 80% more efficient than the water, and Choctaw cultivar showing the highest content. Flavonoids ranged from 0.46 to 1.14 mg EQ g-1 (f.w.) and Guarani cultivar showed the highest content. High linear correlations were found between total phenolics and antioxidant capacity in both extraction solvents. However, linear correlations between anthocyanins and antioxidant capacity were observed only for the aqueous solvent. Ascorbic acid ranged from 87.87 to 134.09 mg 100 g-1, with emphasis on Brazos cultivar. The aqueous extract showed greater ability to scavenge ABTS radical, but the ethanolic extract was more efficient for the DPPH radical. Blackberries produced under Western Paraná conditions showed high levels of antioxidants with emphasis on Arapaho cultivar. This study showed that water and ethanol solvents influence different results on phenolic composition and antioxidant activity of blackberries.

Because grain cultivation in Paraná is becoming unsustainable on small farms, growing fruit should be explored on these production units. Thus, the objective of the present work was to quantify the phenology and production of cultivars of black and redberry plants in the western region of Paraná in the town of Marechal Cândido Rondon, Paraná State, Brazil. The experimental design was in randomized blocks, with four blocks and eleven treatments (blackberry cultivars Arapaho, Xavante, Ébano, Comanche, Caingangue, Choctaw, Tupy, Guarani, Brazos and Cherokee and the redberry plants). In each plot, composed of five plants, the phenological and production data for the 2009/10 and 2010/11 production cycles were collected, and the physicochemical quantification of the fruits in the last production cycle was performed. Most of the cultivars presented production cycles longer than 90 days, with the harvest beginning in late October and extending through late January. ‘Brazos’ presented the highest estimated yield. The Brazos, Guarani and Tupy cultivars produced fruits of higher masses. The redberry presented a high fruit yield and proved an excellent option for cultivation in this region.O cultivo de grãos no Paraná vem se tornando inviável em pequenas propriedades rurais. A fruticultura poderia viabilizar a exploração econômica nessas unidades produtivas. Assim, o objetivo do presente trabalho foi quantificar a produção de cultivares de amoreiras-pretas e vermelha na região Oeste do Estado do Paraná. O trabalho foi realizado no município de Marechal Cândido Rondon, Estado do Paraná. O delineamento experimental foi em blocos ao acaso, com quatro blocos e onze tratamentos (cultivares de amoreiras-pretas Arapaho, Xavante, Ébano, Comanche, Caingangue, Choctaw, Tupy, Guarani, Brazos, Cherokee e a amoreira-vermelha). Em cada parcela, constituída de cinco plantas úteis, foram coletados dados fenológicos e produtivos no ciclo de produção 2009/10 e 2010/11, além da quantificação físico-química dos frutos no último ciclo de produção. A maioria das cultivares apresentou ciclo produtivo superior a 90 dias, com colheitas se iniciando ao final de outubro e se estendendo até final de janeiro. ‘Brazos’ apresentou a maior produtividade estimada. As cultivares Brazos, Guarani e Tupy possibilitaram a produção de frutos de maior massa. A amoreira-vermelha apresentou elevada produção de frutos e poderia ser indicada como opção para o processamento.

The Brazilian ground pearl, Eurhizococcus brasiliensis (Wille, 1922) (Hemiptera: Margarodidae), is a subterranean, polyphagous scale insect native of Southern Brazil that feeds on a variety of different vineyard plant species (Vitis spp.). In this study, it is reported three new plant hosts of the species. In 2007 and 2010, infested plants were documented in the towns of Farroupilha (29°14'34"S, 51°23'20"W) and Vacaria (28°26'30"S, 50°52'59"W) in Rio Grande do Sul, Brazil. Specimens of the ground pearl were found in the roots of three cultivated berry plants: blackberry (Rubus spp.), raspberry (Rubus idaeus L.) and blueberry (Vaccinium sp.). Observed symptoms included: chlorotic leaves, gradual wasting, reduced production, and mortality. Given the increasing popularity of berry orchards in the region, this study serves as an alert for farmers to avoid establishing them in areas infested with the ground pearl and to check for the insects in the root cuttings used to establish berry crops.A pérola-da-terra Eurhizococcus brasiliensis (Wille, 1922) (Hemiptera: Margarodidae) é uma cochonilha subterrânea, polífaga, nativa do sul do Brasil, que se alimenta de diversas espécies vegetais causando prejuízos, principalmente à cultura da videira (Vitis spp.). Nesta comunicação, três espécies vegetais são relatadas como novos hospedeiros da praga. Em 2007 e 2010, plantas infestadas foram registradas nos municípios de Farroupilha (29°14'34"S e 51°23'20"W) e Vacaria (28°26'30"S e 50°52'59"W), RS. Exemplares da cochonilha foram encontrados em raízes de amoreira-preta (Rubus spp.), framboeseira (Rubus idaeus L.) e mirtileiro (Vaccinium sp.). Os sintomas observados foram folhas cloróticas, definhamento progressivo, diminuição na produção e morte das plantas. Devido à ampliação do cultivo de pequenas frutas na região, essa informação serve de alerta aos produtores para que evitem o plantio em áreas infestadas com a cochonilha e observem a presença do inseto no material vegetativo utilizado para estabelecer o cultivo de pequenas frutas.

Este trabalho objetivou verificar o efeito do bioestimulante Promalin® na promoção da brotação em estacas radiciais de amoreira-preta (Rubus spp.) cv. Brazos. O experimento foi conduzido de janeiro a março de 2010, sendo o delineamento inteiramente casualizado, com cinco concentrações de bioestimulante, na forma de solução: T1= 0 mg L-1; T2= 10 mg L-1; T3= 20 mg L-1; T4= 40 mg L-1, e T5= 80 mg L-1, sendo as estacas radiciais mergulhadas nessas soluções durante 12 horas, em sete repetições com dez estacas cada. Após 75 dias, foram avaliadas: a emergência de brotos, o número de estacas brotadas, número de brotos, número de folhas, comprimento da haste principal e porcentagem de sobrevivência das brotações, além da análise de carboidratos solúveis. Ocorreu evolução gradativa ao longo do tempo na emergência das brotações, iniciando em aproximadamente sete dias após o plantio. As maiores concentrações de Promalin® inibiram a brotação em estacas de raiz, e as características de desenvolvimento dessas brotações foram alteradas, sendo os melhores resultados alcançados sem a aplicação dos reguladores vegetais.

O objetivo do presente trabalho foi avaliar o enraizamento de estacas caulinares e radiculares de cultivares de amoreira-preta, coletadas em diferentes épocas, armazenadas a frio e tratadas com AIB. No primeiro experimento, estacas radiculares e caulinares da amoreira-preta 'Tupy' foram coletadas no momento da poda hibernal, realizada nas seguintes épocas: 07/06, 22/06, 08/07, 22/07, 06/08 e 20/08 de 2009. Já no segundo experimento, metade das estacas caulinares e radiculares da mesma cultivar foi armazenada a frio por 30 dias e a outra metade das estacas foi colocada diretamente para enraizar. Todas as estacas foram tratadas com diferentes concentrações de ácido indolbutírico (AIB): 1000, 2000, 3000 e 4000mg L-1 por 10s., além do controle composto somente por água. No terceiro experimento, foram coletadas estacas radiculares das cultivares 'Choctaw', 'Ébano', 'Guarani', 'Arapaho', 'Brazos', 'Cherokee', 'Comanche', 'Caingangue', 'Tupy' e 'Xavante' na poda realizada no dia 22 de junho do ano seguinte. As estacas foram armazenadas a frio por 30 dias e não foi realizado tratamento com AIB. Em ambos os experimentos, as estacas caulinares foram enterradas 2/3 de seu comprimento na posição vertical e as estacas radiculares foram totalmente imersas na posição horizontal, utilizando a vermiculita de grânulos finos como substrato, em telado com sombreamento de 50%. Após 90 dias, pode-se concluir que as estacas radiculares apresentam melhores resultados, devendo ser armazenadas a frio e não tratadas com AIB, mas há diferença do potencial propagativo entre os cultivares de amoreira-preta.The objective of the present research was to quantify the rizogenic potential of stems and root cuttings of blackberry cultivars, collected in different times, cold storage and treated with indolbutyric acid. In the first experiment, roots and stems cuttings of 'Tupy' blackberry were collected close to it hibernal prune and accomplished in the following times: 07/06, 22/06, 08/07, 22/07, 06/08 and 20/08 of 2009. Already in the second experiment, half of the stems and roots cuttings of 'Tupy' blackberry were submitted to cold storage for 30 days and the other amount of cuttings were placed directly for rooting. The whole cuttings were treated with different concentrations of indolbutyric acid (IBA): 1000, 2000, 3000 and 4000mg L-1 for 10 seconds, and control only for water. In the third experiment, roots cuttings of 'Choctaw', 'Ébano', 'Guarani', 'Arapaho', 'Brazos', 'Cherokee', 'Comanche', 'Caingangue', 'Tupy' and 'Xavante' blackberry cultivars were collected in the pruning accomplished in june 22 of the following year. The cuttings were cold storage for 30 days and treatment was not accomplished with IBA. In both experiments, the stems cuttings were buried 2/3 of its length in the vertical position and the roots cuttings were totally immerged in the horizontal position, using vermiculita as substrate, in greenhouse with 50% of light. After 90 days, it can be concluded that the roots cuttings presents better results, owing cold storage and without IBA, but there is difference of propagation potential among blackberry cultivars.

A amora-preta é uma opção importante para fruticultura paranaense, porém não há informações a respeito do cultivo dessa frutífera nas condições subtropicais do Estado. O objetivo deste trabalho foi avaliar a influência da época de poda na produção da amoreira-preta 'Tupy'. O trabalho foi realizado em um pomar comercial, conduzido em sistema agroecológico. O delineamento experimental foi em blocos ao acaso, com quatro blocos e seis tratamentos (podas quinzenais realizadas durante o inverno). Em cada parcela, constituída de três plantas úteis, foram coletados dados fenológicos, produtivos e físico-químicos no ciclo de produção 2008/09 e 2009/10. Podas efetuadas no início de julho são as mais indicadas e podas tardias podem prejudicar o desempenho produtivo das amoreiras-pretas 'Tupy' no oeste do Paraná.The blackberry is an important option to fruit growers in Paraná State. However, there aren't trials performed in subtropical area in that State. The objective of this research was to evaluate the pruning time effects at season production of 'Tupy' blackberry. The research was conducted in a commercial orchard, in an agroecological system. The experimental design was randomized blocks with four blocks and six treatments (pruning time by fifteen days during the winter pruning). In each plot, which was consisted of three plants, it was collected phenological, productive and physical-chemical production cycle in 2008/09 and 2009/10. Pruning made in early July are the most suitable and late pruning can harm the productive performance of blackberry 'Tupy' in western Paraná.

A propagação da amoreira-preta e da framboeseira dá-se principalmente por meio de estacas de raiz e mesmo de hastes novas, contudo, já é crescente o interesse pelo uso da micropropagação como um método alternativo de propagação . O enraizamento é uma das etapas mais difícieis, onde a definição do meio de cultivo, da concentração ótima de AIB para o enraizamento, constitui um passo importante, por isso objetivou-se com este experimento determinar o melhor meio de cultivo, melhor tipo de cultivo e a melhor concentração de AIB no meio de cultura para o enraizamento in vitro da amoreira-preta 'Xavante' e de framboeseira 'Batum' e 'Heritage'. O material vegetal utilizado foram microestacas apicais com duas folhas, com cerca de 1 cm de comprimento, oriundas do cultivo in vitro. Os fatores estudados foram o tipo de meio de cultura MS e WPM - Wood Plant Media, a concentração de AIB no meio de cultura e o tempo de cultivo das microestacas em meio com AIB. O meio WPM, em concentrações baixas, menores de 3 µM de AIB, induziram maiores médias de enraizamento e comprimento. Concentrações altas de AIB induziram a formação de calo, para amoreira-preta, 'Xavante'. Para a framboeseira o meio WPM, com menores concentrações de AIB (0 e 3 µM), mostrou as melhores médias no número de raízes, comprimento de raízes e pequena intensidade de calo; com as maiores concentrações de AIB, ocorreu maior aparecimento de calo.The propagation of blackberry and raspberry occur mainly through root cutting and even of softwoods stem; however, it is already increasing the interest for the use of the micropropagation as an alternative technique of vegetative propagation of fruit plants, since the rooting is one of the most difficult stages, in which the definition of both the culture environment and the best concentration of AIB for the rooting, is an important stage. For that purpose, the aim of this research was to determine the best cultivation type and the best concentration of IBA in the culture environment for the in vitro rooting of the blackberry 'Xavante' and the raspberry 'Batum' and 'Heritage'. The vegetable material used was atex microcutting with two leaves, measuring about 1 cm of length, originated from the in vitro cultivation. The studied factors were the kinds of culture (MS and WPM), the IBA concentration in the culture environment and the time of cultivation of the microcutting in an environment with IBA. The WPM environment, in low concentrations, less than 3 µM of IBA, together with T1, induced larger rooting averages and length. High concentrations of AIB induced the callus formation for blackberry 'Xavante'. For raspberry, using WPM environment with smaller concentrations of AIB (0 and 3 , µM), it was shown the best average in the number of roots, length of roots and small callus intensity, with the largest concentrations of IBA it happened larger callus emergence.

Desenvolveu-se, este trabalho, no intuito de aprimorar técnicas de propagação in vitro de amoreira-preta. Testou-se em um experimento a influência de cinco diferentes concentrações de ANA (0; 0,1; 0,5; 1,0 e 1,5 mg L-1) e cinco de GA3 (0; 2; 4; 6 e 8 mg L-1), adicionadas ao meio de cultura MS, sob a amoreira-preta cultivar Ébano e; num segundo experimento testaram-se seis diferentes concentrações de ANA (0; 0,1; 0,5; 1,0; 1,5 e 2,0 mg L-1) e duas cultivares de amoreira-preta (Tupy e Brazos), no crescimento in vitro de plântulas. Segmentos nodais com 2 cm, de plântulas preestabelecidas in vitro, foram excisadas e inoculadas, em meio MS. O experimento foi inteiramente casualisado, utilizando-se três explantes por repetição e quatro repetições por tratamento. O pH do meio foi ajustado para 5,8 depois da adição de 6 g L-1 de ágar e 30 g L-1 de sacarose, ocorrendo depois a autoclavagem a 121ºC e 1 atm por 20 minutos. Após a inoculação, os tubos de ensaio foram mantidos por 60 dias, em sala de crescimento a 27 ± 1ºC, irradiância de 35 mmol.m-2.s-1 e fotoperíodo de 16 horas, avaliando-se assim o número de folhas, número de raízes, comprimento da maior raiz, comprimento da parte aérea, peso da matéria fresca e seca da parte aérea. Altas concentrações de GA3 associadas a baixas de ANA promoveram maior comprimento da parte aérea da amoreira-preta, cultivar Ébano. Maior comprimento da parte aérea de 'Brazos' foi verificado na presença de 1,0 mg L-1 de ANA. Verificou-se surgimento de calos na cultivar Ébano em todas as concentrações de GA3 associadas a 0,5-1,5 mg L-1 de ANA e nas cultivares Tupy e Brazos em todas as concentrações de ANA. Melhores resultados na micropropagação da amoreira-preta cultivares Tupy e Ébano foram obtidos com a adição de 1,0 mg L-1 de ANA e melhores resultados no enraizamento da amoreira-preta cultivar Ébano foram obtidos com baixas concentrações de ANA e na ausência de GA3.This work was developed with the aim of improving technics of in vitro propagation of blackberry. So, one tested in an experiment the influence of five different ANA concentrations (0; 0,1; 0,5; 1,0 and 1,5 mg L-1) and five AG3 (0; 2,0; 4,0; 6,0 and 8,0 mg L-1), added to the culture medium MS, to the blackberry cv. Ebano and; in a second experiment was tested six different ANA concentrations (0; 0,1; 0,5; 1,0; 1,5 and 2,0 mg L-1) and two blackberry cv. (Tupy and Brazos), in vitro plants growing. Nodal segments with 2 cm, of in vitro plants, were excised and inoculated, in a MS culture medium. The experiment was entirely randomized blocks using three explants by repetition and four repetitions per treatment. The pH of the culture medium was adjusted for 5.8 after the addition of 6 g L-1 agar and 30 g L-1 sucrose, happening sterilization later at 121ºC and 1 atm per 20 minutes. After the inoculation, the tubes were maintained by 60 days, in growth room at 27 ± 1ºC, irradiance of 35 mol.m-2.s-1 and photoperiod of 16 hours, being evaluated the number of leaves, number of roots, length of the largest root, length of the aerial part, dry weight of the fresh and dry matter of the aerial part. High concentrations of AG3 associated to low concentrations of ANA promoted larger length of the aerial part of the blackberry cv. Ebano. Larger length of the aerial part of ' Brazos' was verified in the presence of 1.0 mg L-1 ANA. Appearance of callus was verified in blackberry cv. Ebano in all the AG3 concentrations associated to 0.5-1.5 mg L-1 ANA and in cv. Tupy and Brazos in all the ANA concentrations. Better results in the blackberry micropropagation cv. Tupy and Ebano were obtained with the addition of 1.0 mg L-1 ANA and better results in the blackberry rooting cv. Ebano were obtained with low ANA concentrations and AG3 absence.

Este trabalho foi realizado com o objetivo de avaliar o potencial de multiplicação in vitro de sete cultivares de amoreira-preta: Brazos, Cherokee, Comanche, Ébano, Guarani, Tupy e Xavante. Utilizou-se protocolo da Embrapa Clima Temperado empregado em laboratórios comerciais. A desinfestação dos explantes foi realizada em soluções à base de álcool e hipoclorito de sódio; a cultura dos meristemas em meio semi-sólido MS com 1 mg L-1 BAP, 0,01 mg L-1 ANA e 0,1 mg L-1 AG3; a multiplicação em MS com 0,8 mg L-1 BAP; e o enraizamento em ½MS com 0,5 mg L-1 ANA, sempre a 25±4ºC, 20 µE m-2 s-1, e fotoperíodo de 16 horas. Partiu-se de 60 meristemas de cada cultivar, avaliando-se a taxa de multiplicação e os níveis de contaminação, vitrificação e oxidação durante as fases de estabelecimento (60 dias), multiplicação (seis subcultivos de 40 dias) e enraizamento in vitro (30 dias). O número estimado de plantas obtidas por meristema foi de 16.335 para 'Brazos', 24.211 para 'Cherokee', 19.778 para 'Comanche', 106.550 para 'Ébano', 14.275 para 'Guarani', 34.022 para 'Tupy' e 24.651 para 'Xavante'. A quantificação dessa variabilidade de resposta in vitro é importante para o planejamento da produção de mudas de cada cultivar nos laboratórios de micropropagação.The aim of this work was to evaluate the in vitro potential of multiplication of seven blackberry cultivars: Brazos, Cherokee, Comanche, Ébano, Guarani, Tupy and Xavante. A commercial micropropagation protocol from Embrapa Clima Temperado was used. The explant desinfestation was carried out in solution of alcohol and sodium hypochlorite. The meristem culture was carried out in semi-solid MS medium with 1 mg L-1 BAP, 0.01 mg L-1 ANA and 0.1 mg L-1 AG3; the explants multiplication in MS medium with 0.8 mg L-1 BAP; and the rooting in ½MS medium with 0.5 mg L-1 ANA, always at 25±4ºC, 20 µE m-2 s-1 and 16 hours photoperiod. Sixty meristems of each cultivar were used, evaluating the multiplication rate and the level of contamination, vitrification and oxidation during the phases of in vitro establishment (60 days), multiplication (six subcultures of 40 days) and rooting (30 days). The estimated number of plants obtained per meristem was 16335 of 'Brazos', 24211 of 'Cherokee', 19778 of 'Comanche', 106550 of 'Ébano', 14275 of 'Guarani', 34022 of 'Tupy' and 24651 of 'Xavante'. The quantification of this in vitro variability response is important to plan the multiplication of the plants of each cultivar in commercial laboratories.

A micropropagação da amoreira-preta pode gerar plantas livres de vírus e em curto espaço de tempo. Com o objetivo de aprimorar técnicas de micropropagação de amoreira-preta cv. Tupy (Rubus spp.), segmentos nodais com cerca 2 cm e 2 gemas axilares, oriundos de plantas pré estabelecidas in vitro, foram excisados e inoculados em meio de cultura MS, suplementado com diferentes concentrações de fosfato de sódio (0, 125, 250, 500 e 1000 mg L-1) e de cloreto de potássio (0, 125, 250, 500 e 1000 mg L-1). O pH foi ajustado para 5,8 antes da adição de 6 g L-1 de ágar e da autoclavagem a 121ºC e 1 atm por 20 minutos. Após a inoculação, os explantes foram transferidos para sala de crescimento a 25 ± 1ºC, irradiância de 35 mmol m-2 s-1 e fotoperíodo de 16 horas, onde permaneceram por 60 dias. O delineamento experimental utilizado foi inteiramente casualisado, utilizando-se de quatro repetições constituídas de três tubos de ensaio contendo um explante cada. O número de brotos e o comprimento da parte aérea das plantas foi menor em função de maiores concentrações de cloreto de potássio. Melhores resultados foram obtidos na ausência de KCl e na presença de fosfato de sódio, principalmente para comprimento e matéria fresca da parte aérea.The micropropagation of blackberry can generate virus-free plants in short time. In order to improve micropropagation techniques of blackberry cv. Tupy (Rubus spp.), nodal segments with 2 cm length and 2 axillary buds originating from plants in vitro were excised and inoculated in MS culture medium, supplemented with different concentrations of sodium phosphate (0, 125, 250, 500 and 1000 mg L-1) and potassium chloride (0, 125, 250, 500 and 1000 mg L-1). The pH was adjusted to 5.8 before the addition of 6 g L-1 agar and sterilization at 121ºC and 1 atm for 20 minutes. After the inoculation, the explants were transferred to growth room at 25 ± 1ºC, 35 mmol m-2 s-1 irradiance and photoperiod of 16 hours, where they stayed for 60 days. The experiment was arranged in a completely randomized design, using four repetitions with 12 plants each. Number of sprouts and length of the aerial part of plants were smaller, due to larger concentrations of potassium chloride. Better results were obtained in the absence of KCl, and in the presence of sodium phosphate, mainly for length and fresh weight of the aerial part.