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Learning the SQL language for working with relational databases is a fundamental subject for future computer engineers. However, in distance learning contexts or unexpected situations like the COVID-19 pandemic, where students had to follow lectures remotely, they may find it hard to learn. Chatbots are software applications that aim to have conversations with people to help them solve problems or provide support in a specific domain. This paper proposes a chatbot-based learning platform to assist students in learning SQL. A case study has been conducted to evaluate the proposal, with undergraduate computer engineering students using the learning platform to perform SQL queries while being assisted by the chatbot. The results show evidence that students who used the chatbot performed better on the final SQL exam than those who did not. In addition, the research shows positive evidence of the benefits of using such learning platforms to support SQL teaching and learning for both students and lecturers: students use a platform that helps them self-regulate their learning process, while lecturers get interesting metrics on student performance.

Balo's concentric sclerosis (BCS) is a rare subtype of multiple sclerosis. Advanced MRI metrics, such as magnetization transfer ratio (MTR), fractional anisotropy (FA), mean diffusivity (MD), and the ratio of total N-acetylaspartate concentration/total creatine concentration (tNAA/tCr) using proton magnetic resonance spectroscopy (1H-MRS), are commonly used in research studies to investigate the effect of a disease modifying therapy (DMT). We report a patient diagnosed with BCS, receiving ocrelizumab, and provide a comparison of the lesion volume, T1-gadolinium lesion volume, MTR, FA, MD, and MRS metrics at baseline, 6- and 12-month follow-up. There was a reduction in Balo's lesion volume on fluid-attenuated inversion recovery (FLAIR) imaging observed in our patient from baseline (23.925 mL) to 12-month follow-up (2.391 mL), with the largest decrease from baseline to 6-month follow-up (3.650 mL). There was no T1-gadolinium enhancement seen at month 6 and 12. The MTR of the lesion did not change significantly (baseline = 50.9%, 6-month = 49.9%, 12-month =50.1%) but the FA increased from 0.188 (at baseline) to 0.304 (at 6 months), while the 12-month follow-up FA was 0.297. We also noted a reduction in MD from baseline (1.333 × 10−3 mm2/s) to 6-month follow-up (1.037 × 10−3 mm2/s), while the 12-month follow-up MD was 1.086 × 10−3 mm2/s. There was a 10.3% increase in tNAA/tCr from 1.583 (at month 0) to 1.747 (at month 12). Our results demonstrate for the first time a direct effect of ocrelizumab on BCS lesions. To validate our findings, more observations are needed in a larger group of BCS patients.

Abstract Background Drugs with anticholinergic properties are associated with cognitive adverse effects, especially in patients vulnerable to central muscarinic antagonism. A variety of drugs show weak, moderate or strong anticholinergic effects. Therefore, the cumulative anticholinergic burden should be considered in patients with cognitive impairment. This study aimed to develop a Swedish Anticholinergic Burden Scale (Swe-ABS) to be used in health care and research. Methods A systematic literature review was conducted in PubMed and Ovid Embase to identify previously published tools quantifying anticholinergic drug burden (i.e., exposure). Drugs and grading scores (0–3, no to high anticholinergic activity) were extracted from identified lists. Enteral and parenteral drugs authorized in Sweden were included. Drugs with conflicting scores in the existing lists were assessed by an expert group. Two drugs that were not previously assessed were also added to the evaluation process. Results The systematic literature search identified the following nine anticholinergic burden scales: Anticholinergic Activity Scale, Anticholinergic Burden Classification, updated Anticholinergic Cognitive Burden scale, Anticholinergic Drug Scale, Anticholinergic Load Scale, Anticholinergic Risk Scale, updated Clinician-rated Anticholinergic Scale, German Anticholinergic Burden Scale and Korean Anticholinergic Burden Scale. A list of drugs with significant anticholinergic effects provided by The Swedish National Board of Health and Welfare was included in the process. The suggested Swe-ABS consists of 104 drugs scored as having weak, moderate or strong anticholinergic effects. Two hundred and fifty-six drugs were listed as having no anticholinergic effects based on evaluation in previous scales. In total, 62 drugs were assessed by the expert group. Conclusions Swe-ABS is a simplified method to quantify the anticholinergic burden and is easy to use in clinical practice. Publication of this scale might make clinicians more aware of drugs with anticholinergic properties and patients’ total anticholinergic burden. Further research is needed to validate the Swe-ABS and evaluate anticholinergic exposure versus clinically significant outcomes.

Abstract Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9. Despite being one of the most robust Cas12a nucleases, LbCas12a in general is less efficient than SpCas9 for genome editing in human cells, animals, and plants. Results To improve the editing efficiency of LbCas12a, we conduct saturation mutagenesis in E. coli and identify 1977 positive point mutations of LbCas12a. We selectively assess the editing efficiency of 56 LbCas12a variants in human cells, identifying an optimal LbCas12a variant (RVQ: G146R/R182V/E795Q) with the most robust editing activity. We further test LbCas12a-RV, LbCas12a-RRV, and LbCas12a-RVQ in plants and find LbCas12a-RV has robust editing activity in rice and tomato protoplasts. Interestingly, LbCas12a-RRV, resulting from the stacking of RV and D156R, displays improved editing efficiency in stably transformed rice and poplar plants, leading to up to 100% editing efficiency in T 0 plants of both plant species. Moreover, this high-efficiency editing occurs even at the non-canonical TTV PAM sites. Conclusions Our results demonstrate that LbCas12a-RVQ is a powerful tool for genome editing in human cells while LbCas12a-RRV confers robust genome editing in plants. Our study reveals the tremendous potential of these LbCas12a variants for advancing precision genome editing applications across a wide range of organisms.

This paper presents an extended work on the Finite Element Method (FEM) simulation of Love Wave (LW) sensors in a liquid medium. Two models are proposed to simulate the multiphysical response of the sensor. Both are extensively described in terms of principle, composition and behavior, making their applications easily reproducible by the sensor community. The first model is a Representative Volume Element (RVE) simulating the transducer and the second focuses on the sensor’s longitudinal (OXZ) cut which simulates the multiphysical responses of the device. Sensitivity of the LW device to variations in the rheological and dielectric properties of liquids is estimated and then compared to a large set of measurements issued from LW sensors presenting different technological characteristics. This integral approach allows for a deeper insight into the multiphysical behavior of the LW sensor. This article also explores the advantages and drawbacks of each model. Both are in good accordance with the measurements and could be used for various applications, for which a non-exhaustive list is proposed in the conclusion.

Feng et al. compare the genome-wide binding and activity of two Drosophila Hox transcription factors that determine the identities of different fly legs. For binding events that are Hox specific, two cofactors, Exd and Dll, are used to achieve specificity in non-overlapping leg domains.

Tuberous sclerosis complex (TSC) is a genetic neurocutaneous disorder that presents with multi-organ involvement, including but not limited to hamartomas in the brain, eyes, heart, lung, liver, kidney, and skin. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory, autoimmune, demyelinating, central nervous system disorder, targeting the optic nerves and spinal cord. We report a 30-year-old woman with TSC who developed tingling in the legs that gradually involved her abdomen. Additional symptoms included severe vomiting that lasted for a week and spasms in her legs. One month later, she was hospitalized due to difficulty ambulating and tingling in her hands. Magnetic resonance imaging (MRI) of her spine showed longitudinally extensive upper cervical and lower thoracic cord signal changes. MRI scan of her brain showed few non-specific T2 signal changes along with cortical and subcortical tubers. Aquaporin (AQP4) IgG antibody was found to be positive in both serum and cerebrospinal fluid. Accordingly, she was diagnosed with NMOSD, treated with a 5-day course of intravenous steroids, followed by 5 sessions of plasma exchange. After her initial improvement, she was started on rituximab as maintenance therapy. Two years later, she is clinically stable, and her follow-up MRI showed marked improvement.

The utility of AsCas12a can be limited to poor editing efficiency. Here the authors identify a variant, “AsCas12a Ultra”, that has high on-target specificity demonstrated through editing of clinically relevant T cell genes.

Purpose: The purpose of this study was to investigate whether there were indications of restrictions to voice function in our group of gender-diverse people assigned female at birth (GD people[superscript AFAB]) and whether the participants would benefit from professional voice support. Method: We conducted a descriptive, cross-sectional, mixed-methods study combining qualitative content analyses of semistructured interviews, phoniatric examinations, and acoustical voice analyses. Fourteen German-speaking GD peopleAFAB participated. The data were examined for indications of restrictions to voice function according to the results of participant self-evaluation, auditory-perceptual analyses, laryngostroboscopic examinations, and acoustical measurements. Results: All participants presented with indications of restrictions to voice function, with vocal power, voice quality, singing voice, laryngeal function, pitch range/variability, and vocal control having found to be most frequently affected (in over 60% of participants). Sixty-four percent of participants expressed an interest in professional voice support, with clinicians and researchers recommending professional voice support for 67% and 71%, respectively. Conclusions: GD people[superscript AFAB] may experience restrictions to a range of domains of voice function and request or be recommended to participate in professional voice support. Research into this area needs to be intensified so that a more in-depth understanding of the potential voice problems GD people[superscript AFAB] might experience can be produced and members of this group can be provided with more comprehensive, evidence-based, and high-quality professional support if and when they need it.

This paper evaluates the effects of circuit training (CT) and super circuit training (SCT) on skill-related fitness components in youth athletes. Skill-related fitness encompasses agility, balance, coordination, speed, power, and reaction time, which are crucial for optimal athletic performance. As youth sports participation continues to rise, effective training interventions that enhance these fitness components are essential for fostering athletic development and reducing injury risks. The study reveals that both CT and SCT are effective methods for improving skill-related fitness in youth athletes. CT typically involves performing a series of exercises targeting different muscle groups in succession, while SCT integrates aerobic and anaerobic exercises into a single workout, often incorporating high-intensity intervals. This review synthesizes existing research to compare the efficacy of these training modalities on various skill-related fitness attributes. Findings indicate that SCT may provide superior benefits for improving cardiovascular endurance and power, while CT is particularly effective for enhancing agility and balance. Furthermore, incorporating functional movements in both training formats encourages engagement and motivation among young athletes. Despite promising results, the study highlights the need for further longitudinal studies to assess the long-term impacts of these training modalities on skill retention and athletic performance. Additionally, the influence of individual factors, such as age, sex, and baseline fitness levels, warrants consideration in future research. Ultimately, this study serves as a foundation for coaches, trainers, and sports organizations to implement evidencebased training programs that maximize the skill-related fitness of youth athletes, thereby promoting healthy development and athletic excellence. [ABSTRACT FROM AUTHOR]

This research paper provides a comparative analysis of assisted versus resisted sprint training, focusing on their effects on acceleration, speed, and biomechanical efficiency in athletes. Sprinting performance is critical in various sports, making the optimization of training methods essential for enhancing athletic capabilities. Assisted sprint training, which utilizes methods such as bungee cords or downhill running, aims to improve acceleration and speed by reducing the resistance faced by the athlete during sprints. Conversely, resisted sprint training involves the application of resistance through techniques like weighted sleds or resistance bands to develop strength and power during the sprinting motion. The paper synthesizes findings from multiple studies to examine the physiological and biomechanical effects of these training modalities. Key performance indicators such as acceleration time, maximum speed, and biomechanical efficiency are evaluated to ascertain which method yields superior results. The analysis reveals that while both training types contribute positively to sprint performance, their mechanisms differ significantly. Assisted sprint training primarily enhances acceleration and speed through improved stride length and frequency, while resisted training focuses on developing strength and power, resulting in better biomechanical efficiency. Additionally, the paper highlights practical implications for coaches and athletes in selecting appropriate training methods based on individual needs and performance goals. Understanding the comparative effectiveness of assisted and resisted sprint training is crucial for optimizing training regimens, enhancing athletic performance, and minimizing injury risks. This paper ultimately contributes to the existing literature by providing a comprehensive overview of these training strategies and their implications for sprint performance in athletes. [ABSTRACT FROM AUTHOR]