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4 results on '"Rubén Ayala-Suárez"'

1. Elite controllers long-term non progressors present improved survival and slower disease progression

Author

Laura Capa, Rubén Ayala-Suárez, Humberto Erick De La Torre Tarazona, Juan González-García, Jorge del Romero, José Alcamí, and Francisco Díez-Fuertes

Subjects
Medicine, Science
Abstract

Abstract Different phenotypes exhibiting no evidences of disease progression have been described in ART-naïve HIV-1 positive individuals. Long-term non progressors (LTNP) and elite controllers (EC) are low frequent examples of immunological and virological control in HIV-1 positive subjects, respectively. The combination of both phenotypes is even less frequent and studied despite being considered as models of HIV-1 functional cure. A multicenter, prospective study in retrospect including clinical and epidemiological data collected from 313 LTNP of 21 Spanish hospitals was carried out. LTNPs maintaining CD4+ T cell counts over 500 cells/µl and viral loads (VL) under 10,000 copies/mL for at least 10 years in the absence of antiretroviral therapy were followed for a median of 20.8 years (IQR = 15.6–25.5). A 52.1% were considered EC (undetectable VL) and LTNP (EC-LTNP) and a total of 171 (54.8%) and 42 (13.5%) out of the 313 participants maintained LTNP status for at least 20 and 30 years, respectively. EC-LTNP showed lower CD4+ T cell count loss (9.9 vs 24.2 cells/µl/year), higher CD4/CD8 ratio (0.01 vs − 0.09 in ratio), and lesser VL increase (no increase vs 197.2 copies/mL/year) compared with LTNPs with detectable VL (vLTNP). Survival probabilities for all-cause mortality at 30 years from HIV + diagnosis were 0.90 for EC-LTNP and 0.70 for vLTNP (p = 2.0 × 10−3), and EC-LTNP phenotype was the only factor associated with better survival in multivariate analyses (HR = 0.28; 95% CI 0.10–0.79). The probability to preserve LTNP status at 30 years was 0.51 for EC-LTNP and 0.18 for vLTNP (p

Published
2022
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2. Omic Technologies in HIV: Searching Transcriptional Signatures Involved in Long-Term Non-Progressor and HIV Controller Phenotypes

Author

Erick De La Torre-Tarazona, Rubén Ayala-Suárez, Francisco Díez-Fuertes, and José Alcamí

Subjects
transcriptome (RNA-seq), HIV infection, long-term non-progressor (LTNP), HIV controllers (HIC), omics, immune cells, Immunologic diseases. Allergy, RC581-607
Abstract

This article reviews the main discoveries achieved by transcriptomic approaches on HIV controller (HIC) and long-term non-progressor (LTNP) individuals, who are able to suppress HIV replication and maintain high CD4+ T cell levels, respectively, in the absence of antiretroviral therapy. Different studies using high throughput techniques have elucidated multifactorial causes implied in natural control of HIV infection. Genes related to IFN response, calcium metabolism, ribosome biogenesis, among others, are commonly differentially expressed in LTNP/HIC individuals. Additionally, pathways related with activation, survival, proliferation, apoptosis and inflammation, can be deregulated in these individuals. Likewise, recent transcriptomic studies include high-throughput sequencing in specific immune cell subpopulations, finding additional gene expression patterns associated to viral control and/or non-progression in immune cell subsets. Herein, we provide an overview of the main differentially expressed genes and biological routes commonly observed on immune cells involved in HIV infection from HIC and LTNP individuals, analyzing also different technical aspects that could affect the data analysis and the future perspectives and gaps to be addressed in this field.

Published
2022
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3. Insight in miRNome of Long-Term Non-Progressors and Elite Controllers Exposes Potential RNAi Role in Restraining HIV-1 Infection

Author

María Gracia-Ruíz de Alda, Laura Capa, Esther Calonge, Rubén Ayala-Suárez, José Alcamí, Humberto Erick de la Torre Tarazona, Francisco Díez-Fuertes, Unión Europea. Comisión Europea. H2020, Instituto de Salud Carlos III, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Ministerio de Ciencia, Innovación y Universidades (España), Instituto de Salud Carlos III - ISCIII, Red Española de Investigación en SIDA, European Regional Development Fund (ERDF/FEDER), European Union, and Horizon 2020

Subjects
ADAM10, miRNA-Seq, lcsh:Medicine, Viremia, HIV-1 infection, Peripheral blood mononuclear cell, ong-term non-progressors, Article, differential expression, elite controllers, 03 medical and health sciences, 0302 clinical medicine, Immune system, RNA interference, microRNA, biomarker discovery, Medicine, PI3K/AKT/mTOR pathway, 030304 developmental biology, 0303 health sciences, long-term non-progressors, miRNome, business.industry, lcsh:R, General Medicine, medicine.disease, Phenotype, 030220 oncology & carcinogenesis, Immunology, business
Abstract

Factor de impacto: 4,242 Q1 Long-term non-progressors (LTNP) and elite controllers (EC) represent spontaneous natural models of efficient HIV-1 response in the absence of treatment. The main purposes of this work are to describe the miRNome of HIV-1 infected patients with different extreme phenotypes and identify potentially altered pathways regulated by differentially expressed (DE) miRNAs. The miRNomes from peripheral blood mononuclear cells (PBMCs) of dual phenotype EC-LTNP or LTNP with detectable viremia and HIV-infected patients with typical progression before and after treatment, were obtained through miRNA-Seq and compared among them. The administration of treatment produces 18 DE miRNAs in typical progressors. LTNP condition shows 14 DE miRNA when compared to typical progressors, allowing LTNP phenotype differentiation. A set of four miRNAs: miR-144-3p, miR-18a-5p, miR-451a, and miR-324 is strongly downregulated in LTNP and related to protein regulation as AKT, mTOR, ERK or IKK, involved in immune response pathways. Deregulation of 28 miRNA is observed between EC-LTNP and viremic-LTNP, including previously described anti-HIV miRNAs: miR-29a, associated with LTNP phenotype, and miR-155, targeting different pre-integration complexes such as ADAM10 and TNPO3. A holistic perspective of the changes observed in the miRNome of patients with different phenotypes of HIV-control and non-progression is provided. This study has been conducted within the Spanish AIDS Research Network (RIS), funded by Instituto de Salud Carlos III (Plan Estatal de I+D+I 2013-2016) and co-funded by European Regional Development Fund (ERDF) “A way to build Europe” (RD12/0017/0015 and RD16CIII/0002/0001 projects), and the European Union’s Horizon 2020 Research and Innovation Programme under grant number 681137. The HIV BioBank, integrated in the Spanish AIDS Research Network, is partially funded by the RD16/0025/0019 project as part of the Plan Nacional R + D + I and cofinanced by ISCIII- Subdirección General de Evaluación and el Fondo Europeo de Desarrollo Regional (FEDER). R.A.-S. was supported by the Ministry of Innovation, Science and Universities predoctoral funding (FPU18/05527) and H.E.T.-T. by the ISCIII-PFIS predoctoral program (FI14CIII/00014). Sí

Published
2020

4. Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities

Author

Rubén Ayala Suárez, Juan Miguel Mancera, Hany M. Ibrahim, Antonio Astola González, Gonzalo Martínez-Rodríguez, Asmaa Galal-Khallaf, Khaled Mohammed-Geba, Juan Antonio Martos-Sitcha, Ministerio de Asuntos Exteriores y Cooperación (España), Academy of Scientific Research and Technology (Egypt), and Universidad de Cádiz

Subjects
0301 basic medicine, Mediterranean climate, Fish Proteins, medicine.medical_specialty, endocrine system, Salinity, DNA, Complementary, Physiology, medicine.medical_treatment, Acclimatization, Aquatic Science, Biology, Argyrosomus regius, Biochemistry, Perciformes, 03 medical and health sciences, Internal medicine, medicine, Animals, Amino Acid Sequence, RNA, Messenger, Insulin-Like Growth Factor I, Phylogeny, Osmotic concentration, Base Sequence, prl, Growth factor, actb, 04 agricultural and veterinary sciences, General Medicine, Euryhaline, biology.organism_classification, Prolactin, gh, igf1, qPCR, 030104 developmental biology, Endocrinology, Growth Hormone, 040102 fisheries, 0401 agriculture, forestry, and fisheries, hormones, hormone substitutes, and hormone antagonists
Abstract

Aquaculture industry in the Mediterranean region exhibits a growing interest for the Mediterranean meager Argyrosomus regius. Some preliminary works showed a good growth performance of the species in nearly isosmotic salinities. However, the patterns of alteration of prolactin (Prl) as well as growth hormone (Gh)/insulin growth factor-1 (Igf1) axis at the molecular level are not yet described in this species. Therefore, we cloned and sequenced partial cDNAs for pituitary prolactin (prl) and growth hormone (gh), hepatic insulin-like growth factor (igf1), and β-actin (actb). Expression patterns of these transcripts were tested in juveniles of A. regius acclimated to four different environmental salinities: (1) 5 ‰ (hyposmotic); (2) 12 ‰ (isosmotic); (3) 38 ‰ (hyperosmotic; seawater control); and (4) 55 ‰ (extremely hyperosmotic). All investigated transcripts shared high sequence identities with their counterparts in other perciformes. prl mRNA levels showed inverse pattern with increasing salinities. gh mRNA enhanced significantly in both 12 and 55 ‰ salinity groups in comparison with the control group, while igf1 showed its maximum expression levels under the nearly isosmotic environment. The results indicated clear sensitivity of prl, gh and igf1 to changes in environmental salinity, which can possibly control the euryhalinity capacity of this species., This work was carried out as a part of the Spanish-Egyptian joint project AP/039755/11(Development of molecular, physiological and immunological biomarkers for the detection of stress related to the Mediterranean meager aquaculture Argyrosomus regius) awarded from the Spanish agency of international cooperation (Agencia Española de Cooperación Internacional, AECID) and the Egyptian Academy of Scientific Research and Technology, to Juan Miguel Mancera (Universidad de Cádiz, Spain) and Khaled Mohammed-Geba (Menofia University, Egypt).

Published
2016

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