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Your search keyword '"Ruan, Jian-Wen"' showing total 14 results
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14 results on '"Ruan, Jian-Wen"'

3. Defective STING expression potentiates IL-13 signaling in epithelial cells in eosinophilic chronic rhinosinusitis with nasal polyps

Author

Wang, Hai, Hu, Dan-Qing, Xiao, Qiao, Liu, Yi-Bo, Song, Jia, Liang, Yuxia, Ruan, Jian-Wen, Wang, Zhe-Zheng, Li, Jing-Xian, Pan, Li, Yu, Di, Wang, Hai, Hu, Dan-Qing, Xiao, Qiao, Liu, Yi-Bo, Song, Jia, Liang, Yuxia, Ruan, Jian-Wen, Wang, Zhe-Zheng, Li, Jing-Xian, Pan, Li, and Yu, Di

Abstract

Background: Stimulator of interferon genes (STING) activation favors effective innate immune responses against viral infections. Its role in chronic rhinosinusitis with nasal polyps (CRSwNP) remains unknown. Objective: Our aim was to explore the expression, regulation, and function of STING in CRSwNP. Methods: STING expression in sinonasal mucosal samples was analyzed by means of quantitative RT-PCR, immunohistochemistry, flow cytometry, and Western blotting. Regulation and function of STING expression were explored by using cultured primary human nasal epithelial cells (HNECs) and cells of the line BEAS-2B in vitro. Results: STING expression was reduced in eosinophilic nasal polyps compared with that in noneosinophilic nasal polyps and control tissues. STING was predominantly expressed by epithelial cells in nasal tissue and was downregulated by IL-4 and IL-13 in a signal transducer and activator of transcription 6 (STAT6)-dependent manner. HNECs derived from eosinophilic polyps displayed compromised STING-dependent type I interferon production but heightened IL-13–induced STAT6 activation and CCL26 production as compared with HNECs from noneosinophilic polyps and control tissues, which were rescued by exogenous STING overexpression. Knocking down or overexpressing STING decreased or enhanced expression of suppressor of cytokine signaling 1 (SOCS1) in BEAS-2B cells, respectively, independent of the canonic STING pathway elements TBK1 and IRF3. Knocking down SOCS1 abolished the inhibitory effect of STING on IL-13 signaling in BEAS-2B cells. STING expression was positively correlated with SOCS1 expression but negatively correlated with CCL26 expression in nasal epithelial cells from patients with CRSwNP. Conclusions: Reduced STING expression caused by the type 2 milieu not only impairs STING-dependent type I interferon production but also amplifies IL-13 signaling by decreasing SOCS1 expression in nasal epithelial cells in eosinophilic CRSwNP.

Published
2021

4. Defective STING expression potentiates IL-13 signaling in epithelial cells in eosinophilic chronic rhinosinusitis with nasal polyps

Author

Wang, Hai, primary, Hu, Dan-Qing, additional, Xiao, Qiao, additional, Liu, Yi-Bo, additional, Song, Jia, additional, Liang, Yuxia, additional, Ruan, Jian-Wen, additional, Wang, Zhe-Zheng, additional, Li, Jing-Xian, additional, Pan, Li, additional, Wang, Meng-Chen, additional, Zeng, Ming, additional, Shi, Li-Li, additional, Xu, Kai, additional, Ning, Qin, additional, Zhen, Guohua, additional, Yu, Di, additional, Wang, De-Yun, additional, Wenzel, Sally E., additional, and Liu, Zheng, additional

Published
2021
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6. The activation and function of IL-36γ in neutrophilic inflammation in chronic rhinosinusitis

Author

Wang, Hai, primary, Li, Zhi-Yong, additional, Jiang, Wen-Xiu, additional, Liao, Bo, additional, Zhai, Guan-Ting, additional, Wang, Nan, additional, Zhen, Zhen, additional, Ruan, Jian-wen, additional, Long, Xiao-Bo, additional, Wang, Heng, additional, Liu, Wei-Hong, additional, Liang, Geng-Tian, additional, Xu, Wei-Min, additional, Kato, Atsushi, additional, and Liu, Zheng, additional

Published
2018
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8. The first laboratory-confirmed SFTS case from the Xinjiang Uygur Autonomous Region importing to Hainan International Tourism Island

Author

Lu, Gang, primary, Deng, Fei, primary, Shen, Shu, primary, Zhu, Li-ying, additional, Yin, Fei-fei, additional, Moming, Abulimiti, additional, Wang, Bo, additional, Gao, Li-juan, additional, Ruan, Jian-wen, additional, Wu, Na, additional, Zhang, You, additional, Wang, Hua-lin, additional, and Hu, Zhi-hong, additional

Published
2018
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13. The first laboratory-confirmed SFTS case from the Xinjiang Uygur Autonomous Region importing to Hainan International Tourism Island.

Author

Zhu, Li-ying, Yin, Fei-fei, Moming, Abulimiti, Wang, Bo, Gao, Li-juan, Ruan, Jian-wen, Wu, Na, Zhang, You, Wang, Hua-lin, Hu, Zhi-hong, Lu, Gang, Deng, Fei, and Shen, Shu

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by a tick-borne phlebovirus, which was reported to be continuously epidemic in Japan, Korea and in 23 provinces of China since 2010. Most laboratory-confirmed SFTS cases in China have been recorded in 18 Eastern and Central provinces. A few suspected cases with SFTS-like symptoms were reported to the Chinese Disease Prevention and Control Information System from provinces including Guangxi, Guangdong, Gansu and Xinjiang Uygur Autonomous Region (XJUAR), however diagnosis was not confrimed due to lack of molecular biological and virological evidence. Here we reported the first laboratory-confirmed SFTS case in 2017. A resident of Hainan International Tourism Island (HNITI) was bitten by ticks when traveling in XJUAR and had illness onset after returning to HNITI. RT-PCR detected SFTSV RNA in the patient’s serum samples. Antibodies against SFTSV were detected from the patient and the neutralization from serum samples was evaluated. And the samples of person who had close contact to the patient were also investigated. Moreover, a new SFTSV strain was isolated from the serum sample collected from the patient during acute phase of disease. The viral properties and phylogeny were further characterized. In addition, SFTSV was detected positive in ticks collected from XJUAR in 2017, which suggested that SFTSV was more widely distributed than we recognized. Therefore, this study identified the first SFTS case from XJUAR where confirmed cases have never been reported and demonstrated the substantial risk from SFTSV infection via tick bite there. It is also the first importing SFTS case in HNITI, which showed the significant role of human transport in disease spread and indicated that the recently authorized international tourism island may face more challenges for controlling other importing cases from different areas and countries. [ABSTRACT FROM AUTHOR]

Published
2018
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