Search

The oligotrophic oceanic euphotic zone comprises the upper nutrient-depleted layer (NDL) and the lower nutrient-replete layer (NRL). However, knowledge of microbial metabolic processes in these two distinct regimes is limited. Here, we characterized the functional microbial proteins of two regimes in two contrasting environments, sites SEATS and SS1 located in the north and the center of the oligotrophic South China Sea using a metaproteomic approach. Proteobacteria was the predominant functional group in both the NDL and the NRL, and Alteromonadales, Oceanospirillales and Rhodobacterales were the most abundant proteobacterial functional groups at the order level, while eukaryotic phytoplankton had a more important metabolic role in the NRL. In contrast to the NDL, prokaryotic composition in the NRL significantly differed between the two sites. The more abundant cyanobacteria in the NRL of SEATS were attributed to higher nutrient concentration. Rhodobacterales were mainly associated with aerobic anoxygenic photosynthetic activity in the two regimes and were actively involved in organic carbon degradation. Free-living proteobacterial members along with the mixotrophic picocyanobacteria were the predominant carbon fixers in the NDL. However, microbial CO2 assimilation was more active in the NRL and eukaryotic phytoplankton contributed significantly to carbon fixation in this regime. This study provides insight into the different metabolic processes of microbial community between the NDL and NRL, and photomixotroph- and heterotroph-driven carbon fixation in the NDL, together with phytoplankton-driven carbon fixation in the NRL, determines primary production in the euphotic zone of the oligotrophic SCS.

The principle of phase transformation in ultra-high strength steel for cone crusher was studied by DIL805 thermal mechanical simulate, and the critical temperature was determined. The Austenite continuous cooling transformation (CCT) diagram of the steel was confirmed by thermal expansion curve, dilatometry and metallography. The phase transformation model was established and offered a theory for deciding parameters of heat treatment process. The results proved that: when the cooling rate was under 0.5 °C/s, the structure was mainly Pearlite and Bainite. With the increase of cooling rate, the content of lower Bainite increased. When it came to 1°C/s , Martensite start to transform from Austenite. When the cooling rate is 5°C/s, Pearlite disappears, Bainite and Martensite were in the majority. Meanwhile, the mathematical equations of phase transformation have high degree to fit the experimental results, and the phase transformation model is feasible.

The influence of different annealing cooling rates on microstructure,precipitation of the second phase and mechanical properties of IF steel was studied. The recrystallizing behavior of IF steels was analyzed by batch annealing process. After confirming the recrystallizing temperature of IF steel, the simulations of different continuous annealing cooling rates (5°C/s, 20°C/s, 50°C/s, 200°C/s 500°C/s and 1000°C/s ) were performed. At the same time, combined with TEM and XRD, the precipitating behavior and microstructure texture were analyzed. The results showed that the recrystallization temperature was speculated between 540°C and 560°C. As the cooling rate increased, the grain sizes became smaller and the yield strength increased significantly. And the tensile strength and elongation were more or less static. The tensile strength remained 240MPa and the elongation kept around 50%. With 1000°C/s cooling rate, the sample performed best with the r-value of 3.03. TiN precipitation was observed. The annealed texture is mostly composed of {111} texture.

Background and purpose The aim of this study was to investigate the feasibility of applying a partially randomized patients’ preference (PRPP) trial in the clinical evaluation of acupuncture versus cupping therapy for fibromyalgia. Materials and methods The final study included 126 participants. Participants without a treatment modality preference were randomly assigned to either the cupping therapy group or the acupuncture group. Patients with strong preferences were assigned to their treatment modality of choice. Ashi points were used for treatment. Outcome measures were both qualitative (patient expectation and satisfaction) and quantitative (drop-out rate, pain intensity, quality of life, depression assessment). Results The recruitment of the non-randomized participants was completed 8 months before the randomized participants were recruited. There was no statistical difference related to the grouping method in the adjusted drop-out rate, patient expectation, and satisfaction. Conclusion The PRPP model is suitable for use in the clinical evaluation of non-pharmaceutical therapies.

Numerous fMRI studies have confirmed functional abnormalities in resting-state brain networks in migraine patients. However, few studies focusing on causal relationships of pain-related brain networks in migraine have been conducted. This study aims to explore the difference of Granger causality connection among pain-related brain networks in migraine without aura (MWoA) patients. Twenty two MWoA patients and 17 matched healthy subjects were recruited to undergo resting-state fMRI scanning. Independent component analysis was used to extract pain-related brain networks, and Granger causality analysis to characterize the difference of Granger causality connection among pain-related brain networks was employed. Seven pain-related brain networks were identified, and MwoA patients showed more complex Granger causality connections in comparison with healthy subjects. Two-sample t test results displayed that there was the significant difference between right-frontoparietal network (RFPN) and executive control network (ECN). This study indicates that the specific intrinsic brain Granger causality connectivity among pain-related networks in MwoA patients are affected after long-term migraine attacks.

The continuous annealing process was simulated on the heat treatment system for thin steel sheet (CCT-AY-II). As the recrystallization was known, the annealing temperature and holding time were taken into account to study the influence on microstructure and mechanical properties of 0.08mm ultrathin cold-rolled SPCC steel. Combined with transmission electron microscope (TEM), the precipitation behavior was analyzed. The optical micrographs show that the microstructure of the samples are more similar to batch-annealing steel rather than general specification SPCC steel through continuous annealing. With 5°C/s heating rate and annealed at 860°C for 80s, the sample performs best: the yield strength is 161MPa, the elongation is 29% and the r-value is 1.51. AlN and MnS precipitations are observed in the inner grain and at grain boundary. The annealed textures mostly composed of {111} texture. The highest intensity is centered around the {111} orientation.

Rationale: Ramsay Hunt syndrome in conjunction with cranial polyneuritis is not extensively documented, and is very easily misdiagnosed. Patient concerns: A case of a 53-year-old male with Ramsay Hunt syndrome in conjunction with cranial polyneuritis is presented with early symptoms of vertigo, cephalalgia, and facial palsy, followed by zoster oticus 10 days later. Diagnoses: Diagnosis was challenging as this condition presents with multiple neuropathies, and attempting to diagnose based on clinical symptoms was often misleading. Polymerase chain reaction can be used to test for presence of the virus in the cerebrospinal fluid, followed by targeted drug therapy. Interventions: Acupuncture, in conjunction with fire cupping, bloodletting around the afflicted region on the face, as well as oral consumption of herbal medicine and vitamins for nerve nourishment was given to treat this disease. Outcomes: Due to misdiagnosis resulting in delayed treatment, peripheral facial paralysis was left as the main sequelae, while other symptoms responded quickly to treatment. After a 6-month follow-up, facial palsy was still present. Lessons: Considering that targeted antiviral therapy can be used to increase the effectiveness of treatment, early diagnosis, and timely use of medication is critical.